thermotoga maritima

Summary

Summary: A rod-shaped bacterium surrounded by a sheath-like structure which protrudes balloon-like beyond the ends of the cell. It is thermophilic, with growth occurring at temperatures as high as 90 degrees C. It is isolated from geothermally heated marine sediments or hot springs. (From Bergey's Manual of Determinative Bacteriology, 9th ed)

Top Publications

  1. Huang S, Romanchuk G, Pattridge K, Lesley S, Wilson I, Matthews R, et al. Reactivation of methionine synthase from Thermotoga maritima (TM0268) requires the downstream gene product TM0269. Protein Sci. 2007;16:1588-95 pubmed
    The crystal structure of the Thermotoga maritima gene product TM0269, determined as part of genome-wide structural coverage of T...
  2. Bhatnagar J, Borbat P, Pollard A, Bilwes A, Freed J, Crane B. Structure of the ternary complex formed by a chemotaxis receptor signaling domain, the CheA histidine kinase, and the coupling protein CheW as determined by pulsed dipolar ESR spectroscopy. Biochemistry. 2010;49:3824-41 pubmed publisher
    ..spectroscopy (PDS) have been applied to investigate the structure of a soluble ternary complex formed by Thermotoga maritima CheA (TmCheA), CheW, and receptor signaling domains...
  3. Comfort D, Bobrov K, Ivanen D, Shabalin K, Harris J, Kulminskaya A, et al. Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases. Biochemistry. 2007;46:3319-30 pubmed publisher
    ..This issue was examined for GH27 and GH36 through biochemical analysis of GH36 alpha-galactosidase from Thermotoga maritima (TmGalA)...
  4. Mukherjee A, Sokunbi A, Grove A. DNA protection by histone-like protein HU from the hyperthermophilic eubacterium Thermotoga maritima. Nucleic Acids Res. 2008;36:3956-68 pubmed publisher
    ..We show here that HU from the hyperthermophilic eubacterium Thermotoga maritima HU bends DNA and constrains negative DNA supercoils in the presence of topoisomerase I...
  5. Loveridge E, Evans R, Allemann R. Solvent effects on environmentally coupled hydrogen tunnelling during catalysis by dihydrofolate reductase from Thermotoga maritima. Chemistry. 2008;14:10782-8 pubmed publisher
    ..not viscosity, affects the rate of the hydride-transfer reaction catalysed by dihydrofolate reductase from Thermotoga maritima (TmDHFR), in which quantum-mechanical tunnelling has previously been shown to be driven by protein motions...
  6. Mi R, Abole A, Cao W. Dissecting endonuclease and exonuclease activities in endonuclease V from Thermotoga maritima. Nucleic Acids Res. 2011;39:536-44 pubmed publisher
    ..This study defines the enzymatic nature of the endonuclease and exonuclease activity in endonuclease V from Thermotoga maritima. In addition to its well-known inosine-dependent endonuclease, Tma endonuclease V also exhibits inosine-..
  7. Drögemüller J, Stegmann C, Mandal A, Steiner T, Burmann B, Gottesman M, et al. An autoinhibited state in the structure of Thermotoga maritima NusG. Structure. 2013;21:365-75 pubmed publisher
    ..The crystal structure of Thermotoga maritima NusG (TmNusG) shows a three-domain architecture, comprising well-conserved amino-terminal (NTD) and carboxy-..
  8. LARREA A, Pedroso I, Malhotra A, Myers R. Identification of two conserved aspartic acid residues required for DNA digestion by a novel thermophilic Exonuclease VII in Thermotoga maritima. Nucleic Acids Res. 2008;36:5992-6003 pubmed publisher
    ..To better understand this exonuclease family, we have characterized an ExoVII homolog from Thermotoga maritima. Thermotoga maritima XseA/B homologs TM1768 and TM1769 were co-expressed and purified, and show robust ..
  9. Luchansky M, Der B, D Auria S, Pocsfalvi G, Iozzino L, Marasco D, et al. Amino acid transport in thermophiles: characterization of an arginine-binding protein in Thermotoga maritima. Mol Biosyst. 2010;6:142-51 pubmed publisher
    ..The hyperthermophilic eubacterium Thermotoga maritima was found to encode a highly stable and specific periplasmic arginine-binding protein (TM0593)...

More Information

Publications113 found, 100 shown here

  1. Wu T, Huang C, Ko T, Lai H, Ma Y, Chen C, et al. Diverse substrate recognition mechanism revealed by Thermotoga maritima Cel5A structures in complex with cellotetraose, cellobiose and mannotriose. Biochim Biophys Acta. 2011;1814:1832-40 pubmed publisher
    The hyperthermophilic endoglucanase Cel5A from Thermotoga maritima can find applications in lignocellulosic biofuel production, because it catalyzes the hydrolysis of glucan- and mannan-based polysaccharides...
  2. Reiter N, Osterman A, Torres Larios A, Swinger K, Pan T, MONDRAGON A. Structure of a bacterial ribonuclease P holoenzyme in complex with tRNA. Nature. 2010;468:784-9 pubmed publisher
    ..We report the crystal structure of the Thermotoga maritima RNase P holoenzyme in complex with tRNA(Phe)...
  3. Rychlik M, Chon H, Cerritelli S, Klimek P, Crouch R, Nowotny M. Crystal structures of RNase H2 in complex with nucleic acid reveal the mechanism of RNA-DNA junction recognition and cleavage. Mol Cell. 2010;40:658-70 pubmed publisher
    ..Junction recognition is important for the removal of RNA embedded in DNA and may play an important role in DNA replication and repair. ..
  4. Guymon R, Pomerantz S, Ison J, Crain P, McCloskey J. Post-transcriptional modifications in the small subunit ribosomal RNA from Thermotoga maritima, including presence of a novel modified cytidine. RNA. 2007;13:396-403 pubmed
    ..Post-transcriptional modifications have been studied in the SSU rRNA from Thermotoga maritima (optimal growth 80 degrees C), one of the most deeply branched organisms in the Eubacterial phylogenetic ..
  5. Loveridge E, Allemann R. The temperature dependence of the kinetic isotope effects of dihydrofolate reductase from Thermotoga maritima is influenced by intersubunit interactions. Biochemistry. 2010;49:5390-6 pubmed publisher
    Dihydrofolate reductase from the hyperthermophile Thermotoga maritima (TmDHFR) is unique among structurally characterized chromosomal DHFRs in that it forms a stable homodimer...
  6. Kim J, Kavas M, Fouad W, Nong G, Preston J, Altpeter F. Production of hyperthermostable GH10 xylanase Xyl10B from Thermotoga maritima in transplastomic plants enables complete hydrolysis of methylglucuronoxylan to fermentable sugars for biofuel production. Plant Mol Biol. 2011;76:357-69 pubmed publisher
    ..Here, we describe efficient production of the GH10 xylanase Xyl10B from Thermotoga maritima in transplastomic plants and demonstrate exceptional stability and catalytic activities of the in planta ..
  7. Nordin N, Guskov A, Phua T, Sahaf N, Xia Y, Lu S, et al. Exploring the structure and function of Thermotoga maritima CorA reveals the mechanism of gating and ion selectivity in Co2+/Mg2+ transport. Biochem J. 2013;451:365-74 pubmed publisher
    ..In the present study we report functional evidence for this novel gating mechanism in the Thermotoga maritima CorA together with an improved crystal structure of this CorA to 2.7 Å (1 Å=0.1 nm) resolution...
  8. Jungblut S, Klostermeier D. Adenosine 5'-O-(3-thio)triphosphate (ATPgammaS) promotes positive supercoiling of DNA by T. maritima reverse gyrase. J Mol Biol. 2007;371:197-209 pubmed publisher
    ..b>Thermotoga maritima reverse gyrase relaxes negatively supercoiled DNA in the presence of ADP or the non-hydrolyzable ATP-analog ..
  9. Sun L, Levisson M, Hendriks S, Akveld T, Kengen S, Dijkstra B, et al. Crystallization and preliminary crystallographic analysis of an esterase with a novel domain from the hyperthermophile Thermotoga maritima. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007;63:777-9 pubmed publisher
    A predicted esterase (EstA) with an unusual new domain from the hyperthermophilic bacterium Thermotoga maritima has been cloned and overexpressed in Escherichia coli...
  10. Takenoya M, Ohtaki A, Noguchi K, Endo K, Sasaki Y, Ohsawa K, et al. Crystal structure of 1-deoxy-d-xylulose 5-phosphate reductoisomerase from the hyperthermophile Thermotoga maritima for insights into the coordination of conformational changes and an inhibitor binding. J Struct Biol. 2010;170:532-9 pubmed publisher
    ..we report studies of kinetics and the crystal structures of the thermostable DXR from the hyperthermophile Thermotoga maritima. Unlike the mesophilic DXRs, Thermotoga DXR (tDXR) showed activity only with Mg(2+) at its growth ..
  11. Han D, Norris S, Xu Z. Construction and transformation of a Thermotoga-E. coli shuttle vector. BMC Biotechnol. 2012;12:2 pubmed publisher
    ..A lack of genetic tools has hampered the investigation and application of these organisms. This study aims to develop a genetic transfer system for Thermotoga spp...
  12. Levisson M, van der Oost J, Kengen S. Characterization and structural modeling of a new type of thermostable esterase from Thermotoga maritima. FEBS J. 2007;274:2832-42 pubmed
    A bioinformatic screening of the genome of the hyperthermophilic bacterium Thermotoga maritima for ester-hydrolyzing enzymes revealed a protein with typical esterase motifs, though annotated as a hypothetical protein...
  13. Conners S, Mongodin E, Johnson M, Montero C, Nelson K, Kelly R. Microbial biochemistry, physiology, and biotechnology of hyperthermophilic Thermotoga species. FEMS Microbiol Rev. 2006;30:872-905 pubmed
    ..For the model hyperthermophile Thermotoga maritima, microarrays have been used in comparative genomic hybridization studies to investigate diversity among ..
  14. Kakugawa S, Fushinobu S, Wakagi T, Shoun H. Characterization of a thermostable carboxylesterase from the hyperthermophilic bacterium Thermotoga maritima. Appl Microbiol Biotechnol. 2007;74:585-91 pubmed
    The gene encoding carboxylesterase from the hyperthermophilic bacterium Thermotoga maritima (tm0053) was cloned...
  15. Kim T, Heo S, Ku J, Ban C. Functional properties of the thermostable mutL from Thermotoga maritima. BMB Rep. 2009;42:53-8 pubmed
    ..The properties of a stable MutL homologue were investigated using a thermostable MutL (TmL) from Thermotoga maritima MSB8 and whose size exclusion chromatographic and crosslinking analyses were compatible with a dimeric form ..
  16. Payandeh J, Li C, Ramjeesingh M, Poduch E, Bear C, Pai E. Probing structure-function relationships and gating mechanisms in the CorA Mg2+ transport system. J Biol Chem. 2008;283:11721-33 pubmed publisher
    Recent crystal structures of the CorA Mg(2+) transport protein from Thermotoga maritima (TmCorA) revealed an unusually long ion pore putatively gated by hydrophobic residues near the intracellular end and by universally conserved ..
  17. Le Fourn C, Fardeau M, Ollivier B, Lojou E, Dolla A. The hyperthermophilic anaerobe Thermotoga Maritima is able to cope with limited amount of oxygen: insights into its defence strategies. Environ Microbiol. 2008;10:1877-87 pubmed publisher
    b>Thermotoga maritima, an anaerobic hyperthermophilic bacterium, was found able to grow in the presence of low concentrations of oxygen of up to 0.5% (v/v)...
  18. Hu J, Sharma M, Qin H, Gao F, Cross T. Ligand binding in the conserved interhelical loop of CorA, a magnesium transporter from Mycobacterium tuberculosis. J Biol Chem. 2009;284:15619-28 pubmed publisher
    CorA is a constitutively expressed magnesium transporter in many bacteria. The crystal structures of Thermotoga maritima CorA provide an excellent structural framework for continuing studies...
  19. Zimmer J, Rapoport T. Conformational flexibility and peptide interaction of the translocation ATPase SecA. J Mol Biol. 2009;394:606-12 pubmed publisher
    ..The recent crystal structure of the Thermotoga maritima SecA-SecYEG complex shows the ATPase in a conformation where the nucleotide-binding domains (NBDs) have ..
  20. Ganguly A, del Toro Duany Y, Rudolph M, Klostermeier D. The latch modulates nucleotide and DNA binding to the helicase-like domain of Thermotoga maritima reverse gyrase and is required for positive DNA supercoiling. Nucleic Acids Res. 2011;39:1789-800 pubmed publisher
    ..Thus, the latch coordinates the individual domain activities by modulating the helicase-like domain, and by communicating changes in the nucleotide state to the topoisomerase domain. ..
  21. del Toro Duany Y, Klostermeier D. Nucleotide-driven conformational changes in the reverse gyrase helicase-like domain couple the nucleotide cycle to DNA processing. Phys Chem Chem Phys. 2011;13:10009-19 pubmed publisher
  22. Payandeh J, Pai E. A structural basis for Mg2+ homeostasis and the CorA translocation cycle. EMBO J. 2006;25:3762-73 pubmed publisher
    We describe the CorA Mg(2+) transporter homologue from Thermotoga maritima in complex with 12 divalent cations at 3.7 A resolution...
  23. Huang S, Zhang Y, Zhong J. A thermostable recombinant transaldolase with high activity over a broad pH range. Appl Microbiol Biotechnol. 2012;93:2403-10 pubmed publisher
    ..reading frame TM0295, which encodes a putative transaldolase (TAL) from a hyper-thermophilic microorganism, Thermotoga maritima, was cloned and expressed in Escherichia coli. The enzyme activity of transaldolase at high temperatures (e...
  24. Pfoh R, Li A, Chakrabarti N, Payandeh J, Pomès R, Pai E. Structural asymmetry in the magnesium channel CorA points to sequential allosteric regulation. Proc Natl Acad Sci U S A. 2012;109:18809-14 pubmed publisher
    ..b>Thermotoga maritima CorA (TmCorA) is the only member of this protein family whose complete 3D fold is known...
  25. Li X, Fleetwood A, Bayas C, Bilwes A, Ortega D, Falke J, et al. The 3.2 Å resolution structure of a receptor: CheA:CheW signaling complex defines overlapping binding sites and key residue interactions within bacterial chemosensory arrays. Biochemistry. 2013;52:3852-65 pubmed publisher
    ..A new 3.2 Å resolution crystal structure of a Thermotoga maritima MCP protein interaction region in complex with the CheA kinase-regulatory module (P4-P5) and adaptor ..
  26. Eshaghi S, Niegowski D, Kohl A, Martinez Molina D, Lesley S, Nordlund P. Crystal structure of a divalent metal ion transporter CorA at 2.9 angstrom resolution. Science. 2006;313:354-7 pubmed
    ..We have determined a 2.9 angstrom resolution structure of CorA from Thermotoga maritima that reveals a pentameric cone-shaped protein...
  27. Hamel D, Zhou H, Starich M, Byrd R, Dahlquist F. Chemical-shift-perturbation mapping of the phosphotransfer and catalytic domain interaction in the histidine autokinase CheA from Thermotoga maritima. Biochemistry. 2006;45:9509-17 pubmed
    ..chemical-shift-perturbation mapping to define the interaction of P1 and P3P4 from the hyperthermophile Thermotoga maritima. The observed chemical-shift changes in P1 upon binding suggest that the P1 domain is bound by interactions ..
  28. Ito T, Kiyasu N, Matsunaga R, Takahashi S, Yokoyama S. Structure of nondiscriminating glutamyl-tRNA synthetase from Thermotoga maritima. Acta Crystallogr D Biol Crystallogr. 2010;66:813-20 pubmed publisher
    ..Two GluRS homologues from Thermotoga maritima, TM1875 and TM1351, have been biochemically characterized and it has been clarified that only TM1875 ..
  29. Kim D, Kim H, Lee S, Suh S. Crystal structure of Thermotoga maritima SPOUT superfamily RNA methyltransferase Tm1570 in complex with S-adenosyl-L-methionine. Proteins. 2009;74:245-9 pubmed publisher
  30. Nathania L, Nicholson A. Thermotoga maritima ribonuclease III. Characterization of thermostable biochemical behavior and analysis of conserved base pairs that function as reactivity epitopes for the Thermotoga 23S rRNA precursor. Biochemistry. 2010;49:7164-78 pubmed publisher
    ..The biochemical behavior of RNase III of the hyperthermophilic bacterium Thermotoga maritima was analyzed using purified recombinant enzyme. T...
  31. Kim T, Cha H, Lee H, Heo S, Choi K, Ku J, et al. Structural insights of the nucleotide-dependent conformational changes of Thermotoga maritima MutL using small-angle X-ray scattering analysis. J Biochem. 2009;145:199-206 pubmed publisher
    ..Here, we perform analysis on the thermostable MutL protein found in Thermotoga maritima MSB8 (TmL)...
  32. Johnson H, Hampton E, Lesley S. The Thermotoga maritima Trk potassium transporter--from frameshift to function. J Bacteriol. 2009;191:2276-84 pubmed publisher
    The gene for the Thermotoga maritima Trk potassium transporter component TrkA was originally thought to be a frameshift mutation and not to encode a functional protein...
  33. Lee L, Ginsburg M, Crovace C, Donohoe M, Stock D. Structure of the torque ring of the flagellar motor and the molecular basis for rotational switching. Nature. 2010;466:996-1000 pubmed publisher
    ..This allows us to propose a model of the complete ring and switching mechanism in which conformational changes in FliG reverse the electrostatic charges involved in torque generation. ..
  34. Casino P, Rubio V, Marina A. Structural insight into partner specificity and phosphoryl transfer in two-component signal transduction. Cell. 2009;139:325-36 pubmed publisher
    ..Here, we report the structure of the complex between the entire cytoplasmic portion of Thermotoga maritima class I HK853 and its cognate, RR468, as well as the structure of the isolated RR468, both free and BeF(3)(-..
  35. Boucher N, Noll K. Ligands of thermophilic ABC transporters encoded in a newly sequenced genomic region of Thermotoga maritima MSB8 screened by differential scanning fluorimetry. Appl Environ Microbiol. 2011;77:6395-9 pubmed publisher
    The chromosome of Thermotoga maritima strain MSB8 was found to have an 8,870-bp region that is not present in its published sequence...
  36. Rodionova I, Yang C, Li X, Kurnasov O, Best A, Osterman A, et al. Diversity and versatility of the Thermotoga maritima sugar kinome. J Bacteriol. 2012;194:5552-63 pubmed
    ..of all sugar kinases constituting an extensive and diverse sugar kinome in the thermophilic bacterium Thermotoga maritima. Substrate preferences of 14 kinases mainly from the FGGY and PfkB families were inferred by bioinformatics ..
  37. Xu Q, Kozbial P, McMullan D, Krishna S, Brittain S, Ficarro S, et al. Crystal structure of an ADP-ribosylated protein with a cytidine deaminase-like fold, but unknown function (TM1506), from Thermotoga maritima at 2.70 A resolution. Proteins. 2008;71:1546-52 pubmed publisher
  38. Bouthier de La Tour C, Amrani L, Cossard R, Neuman K, Serre M, Duguet M. Mutational analysis of the helicase-like domain of Thermotoga maritima reverse gyrase. J Biol Chem. 2008;283:27395-402 pubmed publisher
    ..investigated the role of the conserved helicase motifs I, II, V, VI, and Q by generating mutants of the Thermotoga maritima reverse gyrase...
  39. Morar M, Anand R, Hoskins A, Stubbe J, Ealick S. Complexed structures of formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima describe a novel ATP binding protein superfamily. Biochemistry. 2006;45:14880-95 pubmed
    ..We determined five different structures of FGAR-AT from Thermotoga maritima in the presence of substrates, a substrate analogue, and a product...
  40. Dalhus B, Arvai A, Rosnes I, Olsen y, Backe P, Alseth I, et al. Structures of endonuclease V with DNA reveal initiation of deaminated adenine repair. Nat Struct Mol Biol. 2009;16:138-43 pubmed publisher
    ..We solved the crystal structures of Thermotoga maritima EndoV in complex with a hypoxanthine lesion substrate and with product DNA...
  41. Lin J, Gao H, Schallhorn K, Harris R, Cao W, Ke P. Lesion recognition and cleavage by endonuclease V: a single-molecule study. Biochemistry. 2007;46:7132-7 pubmed publisher
    ..The prolonged association and dissociation events in the presence of the Ca2+-Mg2+ combination, as compared to that of Mg2+ alone, support the hypothesis that endo V has two metal binding sites to regulate its enzymatic activities...
  42. Serganov A, Huang L, Patel D. Structural insights into amino acid binding and gene control by a lysine riboswitch. Nature. 2008;455:1263-7 pubmed publisher
    ..by riboswitches, here we present the crystal structure of the 174-nucleotide sensing domain of the Thermotoga maritima lysine riboswitch in the lysine-bound (1.9 ångström (A)) and free (3.1 A) states...
  43. Rodionov D, Kurnasov O, Stec B, Wang Y, Roberts M, Osterman A. Genomic identification and in vitro reconstitution of a complete biosynthetic pathway for the osmolyte di-myo-inositol-phosphate. Proc Natl Acad Sci U S A. 2007;104:4279-84 pubmed
    ..In Thermotoga maritima both candidate genes (in an originally misannotated locus TM1418) form an operon with the inositol-1-..
  44. Hatori Y, Hirata A, Toyoshima C, Lewis D, Pilankatta R, Inesi G. Intermediate phosphorylation reactions in the mechanism of ATP utilization by the copper ATPase (CopA) of Thermotoga maritima. J Biol Chem. 2008;283:22541-9 pubmed publisher
    Recombinant and purified Thermotoga maritima CopA sustains ATPase velocity of 1.78-2.73 micromol/mg/min in the presence of Cu+ (pH 6, 60 degrees C) and 0.03-0.08 micromol/mg/min in the absence of Cu+...
  45. Choi K, Seo J, Park K, Park C, Cha J. Characterization of glycosyl hydrolase family 3 beta-N-acetylglucosaminidases from Thermotoga maritima and Thermotoga neapolitana. J Biosci Bioeng. 2009;108:455-9 pubmed publisher
    The genes encoding beta-N-acetylglucosaminidase (nagA and cbsA) from Thermotoga maritima and Thermotoga neapolitana were cloned and expressed in Escherichia coli in order to investigate whether Thermotoga sp...
  46. Arragain S, Garcia Serres R, Blondin G, Douki T, Clemancey M, Latour J, et al. Post-translational modification of ribosomal proteins: structural and functional characterization of RimO from Thermotoga maritima, a radical S-adenosylmethionine methylthiotransferase. J Biol Chem. 2010;285:5792-801 pubmed publisher
    ..We present spectroscopic evidence that Thermotoga maritima RimO, like MiaB, contains two [4Fe-4S] centers, one presumably bound to three invariant cysteines in the ..
  47. Jongruja N, You D, Kanaya E, Koga Y, Takano K, Kanaya S. The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+-dependent activity. FEBS J. 2010;277:4474-89 pubmed publisher
    b>Thermotoga maritima ribonuclease H (RNase H) I (Tma-RNase HI) contains a hybrid binding domain (HBD) at the N-terminal region...
  48. Xia Y, Lundbäck A, Sahaf N, Nordlund G, Brzezinski P, Eshaghi S. Co2+ selectivity of Thermotoga maritima CorA and its inability to regulate Mg2+ homeostasis present a new class of CorA proteins. J Biol Chem. 2011;286:16525-32 pubmed publisher
    ..Here, we show that Thermotoga maritima CorA (TmCorA) is incapable of regulating the Mg(2+) homeostasis and therefore cannot be the primary Mg(2+) ..
  49. VU A, Wang X, Zhou H, Dahlquist F. The receptor-CheW binding interface in bacterial chemotaxis. J Mol Biol. 2012;415:759-67 pubmed publisher
    ..We used nuclear magnetic resonance to characterize the interaction modes of chemoreceptor and CheW from Thermotoga maritima. We find that chemoreceptor binding surface is located near the highly conserved tip region of the N-..
  50. Ganguly A, del Toro Duany Y, Klostermeier D. Reverse gyrase transiently unwinds double-stranded DNA in an ATP-dependent reaction. J Mol Biol. 2013;425:32-40 pubmed publisher
    ..The unwinding activity may provide and stabilize the single-stranded regions required for strand passage by the topoisomerase domain, either de novo or by expanding already existing unpaired regions that may form at high temperatures...
  51. Hatori Y, Majima E, Tsuda T, Toyoshima C. Domain organization and movements in heavy metal ion pumps: papain digestion of CopA, a Cu+-transporting ATPase. J Biol Chem. 2007;282:25213-21 pubmed
    ..and movements in the reaction cycle of heavy metal ion pumps, CopA, a bacterial Cu+-ATPase from Thermotoga maritima was cloned, overexpressed, and purified, and then subjected to limited proteolysis using papain...
  52. Nicolet Y, Rubach J, Posewitz M, Amara P, Mathevon C, Atta M, et al. X-ray structure of the [FeFe]-hydrogenase maturase HydE from Thermotoga maritima. J Biol Chem. 2008;283:18861-72 pubmed publisher
    ..the high resolution structure of recombinant, reconstituted S-adenosine-L-methionine-dependent HydE from Thermotoga maritima. Besides the conserved [Fe(4)S(4)] cluster involved in the radical-based reaction, this HydE was reported ..
  53. Loveridge E, Rodriguez R, Swanwick R, Allemann R. Effect of dimerization on the stability and catalytic activity of dihydrofolate reductase from the hyperthermophile Thermotoga maritima. Biochemistry. 2009;48:5922-33 pubmed publisher
    ..dihydrofolate reductases characterized so far, dihydrofolate reductase (DHFR) from the hyperthermophile Thermotoga maritima forms a highly stable dimer...
  54. Scire A, Marabotti A, Staiano M, Iozzino L, Luchansky M, Der B, et al. Amino acid transport in thermophiles: characterization of an arginine-binding protein in Thermotoga maritima. 2. Molecular organization and structural stability. Mol Biosyst. 2010;6:687-98 pubmed publisher
    ..simulations to investigate the physicochemical properties of a hyperthermophilic binding protein from Thermotoga maritima. We demonstrated preferential binding for the polar amino acid arginine and experimentally monitored the ..
  55. Park C, Yeom S, Lim Y, Kim Y, Oh D. Characterization of a recombinant thermostable L: -rhamnose isomerase from Thermotoga maritima ATCC 43589 and its application in the production of L-lyxose and L-mannose. Biotechnol Lett. 2010;32:1947-53 pubmed publisher
    A putative L-rhamnose isomerase (RhaA) from Thermotoga maritima was purified with a specific activity of 55 U/mg by His-Trap affinity chromatography. The native enzyme was estimated as a 46 kDa tetramer by gel filtration chromatography...
  56. Mahadevan S, Wi S, Kim Y, Lee K, Bae H. In planta differential targeting analysis of Thermotoga maritima Cel5A and CBM6-engineered Cel5A for autohydrolysis. Transgenic Res. 2011;20:877-86 pubmed publisher
    ..We attempted to obtain high-level expression of an intact Thermotoga maritima endoglucanase, Cel5A, and CBM6-engineered Cel5A in transgenic tobacco plants for the mass production and ..
  57. Cheng Y, Ko T, Huang J, Wu T, Lin C, Luo W, et al. Enhanced activity of Thermotoga maritima cellulase 12A by mutating a unique surface loop. Appl Microbiol Biotechnol. 2012;95:661-9 pubmed publisher
    Cellulase 12A from Thermotoga maritima (TmCel12A) is a hyperthermostable ?-1,4-endoglucanase. We recently determined the crystal structures of TmCel12A and its complexes with oligosaccharides...
  58. Rodionova I, Scott D, Grishin N, Osterman A, Rodionov D. Tagaturonate-fructuronate epimerase UxaE, a novel enzyme in the hexuronate catabolic network in Thermotoga maritima. Environ Microbiol. 2012;14:2920-34 pubmed publisher
    b>Thermotoga maritima is a marine hyperthermophilic microorganism that degrades a wide range of simple and complex carbohydrates including pectin and produces fermentative hydrogen at high yield...
  59. Wang Z, Tong W, Wang Q, Bai X, Chen Z, Zhao J, et al. The temperature dependent proteomic analysis of Thermotoga maritima. PLoS ONE. 2012;7:e46463 pubmed publisher
    b>Thermotoga maritima (T. maritima) is a typical thermophile, and its proteome response to environmental temperature changes has yet to be explored. This study aims to uncover the temperature-dependent proteins of T...
  60. Rudolph M, del Toro Duany Y, Jungblut S, Ganguly A, Klostermeier D. Crystal structures of Thermotoga maritima reverse gyrase: inferences for the mechanism of positive DNA supercoiling. Nucleic Acids Res. 2013;41:1058-70 pubmed publisher
    ..We present five structures of authentic Thermotoga maritima reverse gyrase that reveal a first view of two interacting zinc fingers that are crucial for positive DNA ..
  61. Braun F, Marhuenda F, Morin A, Guevel L, Fleury F, Takahashi M, et al. Similarity and divergence between the RNA polymerase alpha subunits from hyperthermophilic Thermotoga maritima and mesophilic Escherichia coli bacteria. Gene. 2006;380:120-6 pubmed
    The alpha subunit (alphaTm) of Thermotoga maritima RNA polymerase has been characterized to investigate its role in transcriptional regulation in one of the few known anaerobic hyperthermophilic bacteria...
  62. Ishii R, Minagawa A, Takaku H, Takagi M, Nashimoto M, Yokoyama S. The structure of the flexible arm of Thermotoga maritima tRNase Z differs from those of homologous enzymes. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007;63:637-41 pubmed
    ..The refined structure of Thermotoga maritima tRNase Z has been determined at 1...
  63. Liebl W, Winterhalter C, Baumeister W, Armbrecht M, Valdez M. Xylanase attachment to the cell wall of the hyperthermophilic bacterium Thermotoga maritima. J Bacteriol. 2008;190:1350-8 pubmed publisher
    ..2.1.8) of the hyperthermophile Thermotoga maritima were investigated, in particular with respect to the unusual outer membrane ("toga") of this gram-negative ..
  64. Zimmer J, Nam Y, Rapoport T. Structure of a complex of the ATPase SecA and the protein-translocation channel. Nature. 2008;455:936-43 pubmed publisher
    ..5 ångström (A), obtained for components from Thermotoga maritima. One copy of SecA in an intermediate state of ATP hydrolysis is bound to one molecule of the SecY complex...
  65. Zhang Y, Thiele I, Weekes D, Li Z, Jaroszewski L, Ginalski K, et al. Three-dimensional structural view of the central metabolic network of Thermotoga maritima. Science. 2009;325:1544-9 pubmed publisher
    ..biology, we generated a three-dimensional reconstruction of the central metabolic network of the bacterium Thermotoga maritima. The network encompassed 478 proteins, of which 120 were determined by experiment and 358 were modeled...
  66. Dalmas O, Cuello L, Jogini V, Cortes D, Roux B, Perozo E. Structural dynamics of the magnesium-bound conformation of CorA in a lipid bilayer. Structure. 2010;18:868-78 pubmed publisher
    The transmembrane conformation of Thermotoga maritima CorA, a magnesium transport system, has been studied in its Mg(2+)-bound form by site-directed spin labeling and electron paramagnetic resonance spectroscopy...
  67. Olszewski M, Grot A, Wojciechowski M, Nowak M, Mickiewicz M, Kur J. Characterization of exceptionally thermostable single-stranded DNA-binding proteins from Thermotoga maritima and Thermotoga neapolitana. BMC Microbiol. 2010;10:260 pubmed publisher
    ..We report the characterization of single-stranded DNA binding proteins (SSBs) from the thermophilic bacteria Thermotoga maritima (TmaSSB) and Thermotoga neapolitana (TneSSB)...
  68. Zhang T, Mu Y. Initial binding of ions to the interhelical loops of divalent ion transporter CorA: replica exchange molecular dynamics simulation study. PLoS ONE. 2012;7:e43872 pubmed publisher
    Crystal structures of Thermotoga maritima magnesium transporter CorA, reported in 2006, revealed its homo-pentameric constructions...
  69. Feng H, Dong L, Cao W. Catalytic mechanism of endonuclease v: a catalytic and regulatory two-metal model. Biochemistry. 2006;45:10251-9 pubmed
    ..mutagenesis to characterize the role of the highly conserved residues D43, E89, D110, and H214 in Thermotoga maritima endonuclease V catalysis...
  70. Hong S, Lee J, Cho K, Math R, Kim Y, Hong S, et al. Construction of the bifunctional enzyme cellulase-beta-glucosidase from the hyperthermophilic bacterium Thermotoga maritima. Biotechnol Lett. 2007;29:931-6 pubmed
    ..enzyme, cellulase-beta-glucosidase, was prepared by gene fusion from the hyperthermophilic bacterium Thermotoga maritima MSB8...
  71. del Toro Duany Y, Jungblut S, Schmidt A, Klostermeier D. The reverse gyrase helicase-like domain is a nucleotide-dependent switch that is attenuated by the topoisomerase domain. Nucleic Acids Res. 2008;36:5882-95 pubmed publisher
    ..Here, the characterization of the isolated helicase-like domain from Thermotoga maritima reverse gyrase is presented...
  72. Cuneo M, Beese L, Hellinga H. Ligand-induced conformational changes in a thermophilic ribose-binding protein. BMC Struct Biol. 2008;8:50 pubmed publisher
  73. Hatori Y, Lewis D, Toyoshima C, Inesi G. Reaction cycle of Thermotoga maritima copper ATPase and conformational characterization of catalytically deficient mutants. Biochemistry. 2009;48:4871-80 pubmed publisher
    ..The copper transport ATPase from Thermotoga maritima (CopA) provides a useful system for mechanistic studies, due to its heterologous expression and stability...
  74. Popp D, Narita A, Maeda K, Fujisawa T, Ghoshdastider U, Iwasa M, et al. Filament structure, organization, and dynamics in MreB sheets. J Biol Chem. 2010;285:15858-65 pubmed publisher
    ..This suggests that the bacterial cell may exploit various nucleotides to generate different filament structures within cables for specific MreB-based functions...
  75. Pereira J, Chen Z, McAndrew R, Sapra R, Chhabra S, Sale K, et al. Biochemical characterization and crystal structure of endoglucanase Cel5A from the hyperthermophilic Thermotoga maritima. J Struct Biol. 2010;172:372-9 pubmed publisher
    ..is an extremely stable enzyme among the endo-acting glycosidases present in the hyperthermophilic organism Thermotoga maritima. Members of GH5 family shows a common (?/?)(8) TIM-barrel fold in which the catalytic acid/base and ..
  76. Schwarzenbacher R, McMullan D, Krishna S, Xu Q, Miller M, Canaves J, et al. Crystal structure of a glycerate kinase (TM1585) from Thermotoga maritima at 2.70 A resolution reveals a new fold. Proteins. 2006;65:243-8 pubmed publisher
  77. McCleverty C, Columbus L, Kreusch A, Lesley S. Structure and ligand binding of the soluble domain of a Thermotoga maritima membrane protein of unknown function TM1634. Protein Sci. 2008;17:869-77 pubmed publisher
    ..Structural Genomics (JCSG) biological targets, the structures of soluble domains of membrane proteins from Thermotoga maritima were pursued...
  78. Garst A, Heroux A, Rambo R, Batey R. Crystal structure of the lysine riboswitch regulatory mRNA element. J Biol Chem. 2008;283:22347-51 pubmed publisher
    ..8 angstroms resolution crystal structure of lysine bound to the Thermotoga maritima asd lysine riboswitch ligand-binding domain...
  79. Morar M, Hoskins A, Stubbe J, Ealick S. Formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima: structural insights into complex formation. Biochemistry. 2008;47:7816-30 pubmed publisher
    ..In this work, a structure of the PurLQS complex from Thermotoga maritima is described revealing a 2:1:1 stoichiometry of PurS:Q:L, respectively...
  80. Cuneo M, Changela A, Miklos A, Beese L, Krueger J, Hellinga H. Structural analysis of a periplasmic binding protein in the tripartite ATP-independent transporter family reveals a tetrameric assembly that may have a role in ligand transport. J Biol Chem. 2008;283:32812-20 pubmed publisher
    ..9 A resolution of a TRAP-PBP (open reading frame tm0322) from the hyperthermophilic bacterium Thermotoga maritima (TM0322)...
  81. Yang X, Ma K. Characterization of a thioredoxin-thioredoxin reductase system from the hyperthermophilic bacterium Thermotoga maritima. J Bacteriol. 2010;192:1370-6 pubmed publisher
    A thioredoxin reductase and a thioredoxin were purified to homogeneity from a cell extract of Thermotoga maritima. The thioredoxin reductase was a homodimeric flavin adenine dinucleotide (FAD)-containing protein with a subunit of 37 kDa ..
  82. Moomaw A, Maguire M. Cation selectivity by the CorA Mg2+ channel requires a fully hydrated cation. Biochemistry. 2010;49:5998-6008 pubmed publisher
    ..The crystal structure of Thermotoga maritima CorA shows a homopentamer with two transmembrane segments per monomer connected by a short periplasmic loop...
  83. Hong S, Lee J, Cho K, Math R, Kim Y, Hong S, et al. Assembling a novel bifunctional cellulase-xylanase from Thermotoga maritima by end-to-end fusion. Biotechnol Lett. 2006;28:1857-62 pubmed
    An artificial, bifunctional, thermostable cellulase-xylanase enzyme from Thermotoga maritima by gene fusion...
  84. Koclega K, Chruszcz M, Zimmerman M, Cymborowski M, Evdokimova E, Minor W. Crystal structure of a transcriptional regulator TM1030 from Thermotoga maritima solved by an unusual MAD experiment. J Struct Biol. 2007;159:424-32 pubmed
    The crystal structure of a putative transcriptional regulator protein TM1030 from Thermotoga maritima, a hyperthermophilic bacterium, was determined by an unusual multi-wavelength anomalous dispersion method at 2...
  85. Bean G, Amann K. Polymerization properties of the Thermotoga maritima actin MreB: roles of temperature, nucleotides, and ions. Biochemistry. 2008;47:826-35 pubmed
    ..We have cloned, overexpressed in Escherichia coli, and purified untagged MreB1 from Thermotoga maritima. We have characterized the conditions that regulate its monomer-to-polymer assembly reaction, the critical ..
  86. Yang C, Rodionov D, Rodionova I, Li X, Osterman A. Glycerate 2-kinase of Thermotoga maritima and genomic reconstruction of related metabolic pathways. J Bacteriol. 2008;190:1773-82 pubmed publisher
    ..The recently reported three-dimensional structure of GK-II from Thermotoga maritima (TM1585; PDB code 2b8n) revealed a new fold distinct from other known kinase families...
  87. Boniface A, Parquet C, Arthur M, Mengin Lecreulx D, Blanot D. The elucidation of the structure of Thermotoga maritima peptidoglycan reveals two novel types of cross-link. J Biol Chem. 2009;284:21856-62 pubmed publisher
    b>Thermotoga maritima is a Gram-negative, hyperthermophilic bacterium whose peptidoglycan contains comparable amounts of L- and D-lysine. We have determined the fine structure of this cell-wall polymer...
  88. Chakrabarti N, Neale C, Payandeh J, Pai E, Pomès R. An iris-like mechanism of pore dilation in the CorA magnesium transport system. Biophys J. 2010;98:784-92 pubmed publisher
    ..This simple and robust mechanism is consistent with the regulation of pore opening by intracellular magnesium concentration, and explains the unusual architecture of CorA...
  89. Offant J, Terrak M, Derouaux A, Breukink E, Nguyen Distèche M, Zapun A, et al. Optimization of conditions for the glycosyltransferase activity of penicillin-binding protein 1a from Thermotoga maritima. FEBS J. 2010;277:4290-8 pubmed publisher
    ..We report here the purification of the ectodomain of the class A penicillin-binding protein 1a from Thermotoga maritima (Tm-1a*), expressed recombinantly in Escherichia coli...
  90. Cheng Y, Ko T, Wu T, Ma Y, Huang C, Lai H, et al. Crystal structure and substrate-binding mode of cellulase 12A from Thermotoga maritima. Proteins. 2011;79:1193-204 pubmed publisher
    Cellulases have been used in many applications to treat various carbohydrate-containing materials. Thermotoga maritima cellulase 12A (TmCel12A) belongs to the GH12 family of glycoside hydrolases...
  91. Motono C, Gromiha M, Kumar S. Thermodynamic and kinetic determinants of Thermotoga maritima cold shock protein stability: a structural and dynamic analysis. Proteins. 2008;71:655-69 pubmed
    The cold shock protein (CSP) from hyperthermophile Thermotoga maritima (TmCSP) is only marginally stable (DeltaG(T(opt)) = 0.3 kcal/mol) at 353 K, the optimum environmental temperature (T(opt)) for T. maritima...
  92. Mahadevan S, Wi S, Lee D, Bae H. Site-directed mutagenesis and CBM engineering of Cel5A (Thermotoga maritima). FEMS Microbiol Lett. 2008;287:205-11 pubmed publisher
    ..In our investigation, the hyperthermostable endoglucanase Cel5A from Thermotoga maritima was subjected to site-directed mutagenesis and carbohydrate-binding module (CBM) engineering...