mycobacterium smegmatis

Summary

Summary: A rapid-growing, nonphotochromogenic species of MYCOBACTERIUM originally isolated from human smegma and found also in soil and water. (From Dorland, 28th ed)

Top Publications

  1. Roy S, Kumari N, Gupta S, Pahwa S, Nandanwar H, Jachak S. 7-Hydroxy-(E)-3-phenylmethylene-chroman-4-one analogues as efflux pump inhibitors against Mycobacterium smegmatis mc² 155. Eur J Med Chem. 2013;66:499-507 pubmed publisher
    ..The compounds 8, 13 and 17 were the most potent inhibitors of ethidium bromide efflux in Mycobacterium smegmatis mc(2) 155.
  2. Gonzalez y Merchand J, Zaragoza Contreras R, Guadarrama Medina R, Helguera Repetto A, Rivera Gutierrez S, Cerna Cortes J, et al. Evaluation of the cell growth of mycobacteria using Mycobacterium smegmatis mc2 155 as a representative species. J Microbiol. 2012;50:419-25 pubmed publisher
    ..Here, we monitored Mycobacterium smegmatis cellular growth by optical density, dry cell mass, and colony forming units; in addition, viability, cell ..
  3. Ta P, Buchmeier N, Newton G, Rawat M, Fahey R. Organic hydroperoxide resistance protein and ergothioneine compensate for loss of mycothiol in Mycobacterium smegmatis mutants. J Bacteriol. 2011;193:1981-90 pubmed publisher
    The mshA::Tn5 mutant of Mycobacterium smegmatis does not produce mycothiol (MSH) and was found to markedly overproduce both ergothioneine and an ~15-kDa protein determined to be organic hydroperoxide resistance protein (Ohr)...
  4. Malik M, Marks K, Mustaev A, Zhao X, Chavda K, Kerns R, et al. Fluoroquinolone and quinazolinedione activities against wild-type and gyrase mutant strains of Mycobacterium smegmatis. Antimicrob Agents Chemother. 2011;55:2335-43 pubmed publisher
    ..mycobacteria, C-8-methoxy dione derivatives were compared with cognate fluoroquinolones by using cultured Mycobacterium smegmatis. Diones exhibited higher MIC values than fluoroquinolones; however, MICs for fluoroquinolone-resistant ..
  5. Xu X, Vilcheze C, Av Gay Y, Gómez Velasco A, Jacobs W. Precise null deletion mutations of the mycothiol synthesis genes reveal their role in isoniazid and ethionamide resistance in Mycobacterium smegmatis. Antimicrob Agents Chemother. 2011;55:3133-9 pubmed publisher
    ..mutants for all four genes involved in the MSH biosynthesis pathway (mshA, mshB, mshC, and mshD) in Mycobacterium smegmatis and made complementing constructs in integrating plasmids...
  6. Maheshwari J, Dharmalingam K. Protective role of Mycobacterium leprae small heat-shock protein in heterologous hosts, Escherichia coli and Mycobacterium smegmatis, grown under stress. J Med Microbiol. 2013;62:959-67 pubmed publisher
    ..coli at heat-shock temperatures. Additional evidence for the protective role of sHsp18 was obtained when Mycobacterium smegmatis harbouring a copy of shsp18 was found to multiply better in human macrophages...
  7. Godbole A, Leelaram M, Bhat A, Jain P, Nagaraja V. Characterization of DNA topoisomerase I from Mycobacterium tuberculosis: DNA cleavage and religation properties and inhibition of its activity. Arch Biochem Biophys. 2012;528:197-203 pubmed publisher
    ..Our studies with Mycobacterium smegmatis topoisomerase I (MstopoI) revealed several of its distinct properties compared to the well studied ..
  8. Rodriguez L, Tirado Y, Reyes F, Puig A, Kadir R, Borrero R, et al. Proteoliposomes from Mycobacterium smegmatis induce immune cross-reactivity against Mycobacterium tuberculosis antigens in mice. Vaccine. 2011;29:6236-41 pubmed publisher
    Proteoliposomes (PL) obtained from Mycobacterium smegmatis (Ms) were evaluated for their capacity to elicit cross-reactive responses against Mycobacterium tuberculosis (Mtb) antigens in BALB/c mice...
  9. Huang Y, Zhou X, Bai Y, Yang L, Yin X, Wang Z, et al. Phagolysosome maturation of macrophages was reduced by PE_PGRS 62 protein expressing in Mycobacterium smegmatis and induced in IFN-? priming. Vet Microbiol. 2012;160:117-25 pubmed publisher
    ..bovis infection. ..

More Information

Publications101 found, 100 shown here

  1. Li Y, Zeng J, Zhang H, He Z. The characterization of conserved binding motifs and potential target genes for M. tuberculosis MtrAB reveals a link between the two-component system and the drug resistance of M. smegmatis. BMC Microbiol. 2010;10:242 pubmed publisher
    ..tuberculosis replication initiator gene, dnaA. However, the dnaA promoter binding sites and many potential target genes for MtrA have yet to be precisely characterized...
  2. Chatrath S, Gupta V, Dixit A, Garg L. The Rv1651c-encoded PE-PGRS30 protein expressed in Mycobacterium smegmatis exhibits polar localization and modulates its growth profile. FEMS Microbiol Lett. 2011;322:194-9 pubmed publisher
    ..In the present study, we investigated the function of PE_PGRS30 by expressing it in Mycobacterium smegmatis. PE_PGRS30 expression in M...
  3. Delogu G, Chiacchio T, Vanini V, Butera O, Cuzzi G, Bua A, et al. Methylated HBHA produced in M. smegmatis discriminates between active and non-active tuberculosis disease among RD1-responders. PLoS ONE. 2011;6:e18315 pubmed publisher
    ..smegmatis is useful to discriminate between active and non-active TB disease among those responsive to QFT-IT in a whole blood system. Further studies are needed to improve the accuracy of the assay. ..
  4. Agarwal N, Pareek M, Thakur P, Pathak V. Functional characterization of EngA(MS), a P-loop GTPase of Mycobacterium smegmatis. PLoS ONE. 2012;7:e34571 pubmed publisher
    ..we characterized the function of a P-loop GTPase in mycobacteria by employing an EngA homologue from Mycobacterium smegmatis, encoded by an open reading frame, designated as MSMEG_3738...
  5. Pecsi I, Hirmondo R, Brown A, Lopata A, Parish T, Vertessy B, et al. The dUTPase enzyme is essential in Mycobacterium smegmatis. PLoS ONE. 2012;7:e37461 pubmed publisher
    ..Using Mycobacterium smegmatis as a fast growing model for Mycobacterium tuberculosis, we demonstrate that dUTPase knock-out results in ..
  6. Nazarova E, Shleeva M, Morozova N, Kudykina Y, Vostroknutova G, Ruzhitsky A, et al. Role of lipid components in formation and reactivation of Mycobacterium smegmatis "nonculturable" cells. Biochemistry (Mosc). 2011;76:636-44 pubmed publisher
    We have found that transition of actively dividing Mycobacterium smegmatis cells into the dormant "nonculturable" state is accompanied by increase in the protein/lipid ratio and disappearance of one of the main lipid components ..
  7. De Leon J, Jiang G, Ma Y, Rubin E, Fortune S, Sun J. Mycobacterium tuberculosis ESAT-6 exhibits a unique membrane-interacting activity that is not found in its ortholog from non-pathogenic Mycobacterium smegmatis. J Biol Chem. 2012;287:44184-91 pubmed publisher
    ..Most importantly, the orthologous ESAT-6 from non-pathogenic Mycobacterium smegmatis (MsESAT-6) was essentially inactive in release of K(+)...
  8. Hayden J, Brown L, Gunawardena H, Perkowski E, Chen X, Braunstein M. Reversible acetylation regulates acetate and propionate metabolism in Mycobacterium smegmatis. Microbiology. 2013;159:1986-99 pubmed publisher
    ..to test whether lysine acetylation and deacetylation impact acetate metabolism in the model mycobacteria Mycobacterium smegmatis, which possesses 25 candidate acetyltransferases and 3 putative lysine deacetylases...
  9. Saikolappan S, Das K, Sasindran S, Jagannath C, Dhandayuthapani S. OsmC proteins of Mycobacterium tuberculosis and Mycobacterium smegmatis protect against organic hydroperoxide stress. Tuberculosis (Edinb). 2011;91 Suppl 1:S119-27 pubmed publisher
    ..OsmC is conserved across mycobacterial species, including Mycobacterium tuberculosis (Rv2923c) and Mycobacterium smegmatis (MSMEG2421), but its role in protecting these species against oxidative stress is unknown...
  10. Jeong J, Baek E, Kim S, Choi J, Oh J. Regulation of the ald gene encoding alanine dehydrogenase by AldR in Mycobacterium smegmatis. J Bacteriol. 2013;195:3610-20 pubmed publisher
    The regulatory gene aldR was identified 95 bp upstream of the ald gene encoding L-alanine dehydrogenase in Mycobacterium smegmatis. The AldR protein shows sequence similarity to the regulatory proteins of the Lrp/AsnC family...
  11. Bashiri G, Rehan A, Greenwood D, Dickson J, Baker E. Metabolic engineering of cofactor F420 production in Mycobacterium smegmatis. PLoS ONE. 2010;5:e15803 pubmed publisher
    ..smegmatis strain. The high levels of the F(420) produced in this study provide a suitable source of this cofactor for studies of F(420)-dependent proteins from other microorganisms and for possible biotechnological applications. ..
  12. Casta eda Garc a A, Do T, Bl zquez J. The K+ uptake regulator TrkA controls membrane potential, pH homeostasis and multidrug susceptibility in Mycobacterium smegmatis. J Antimicrob Chemother. 2011;66:1489-98 pubmed publisher
    ..We have constructed and analysed a library of 11,?000 Mycobacterium smegmatis insertion mutants to find other possible rifampicin-resistance determinants. ..
  13. Sweeney K, Dao D, Goldberg M, Hsu T, Venkataswamy M, Henao Tamayo M, et al. A recombinant Mycobacterium smegmatis induces potent bactericidal immunity against Mycobacterium tuberculosis. Nat Med. 2011;17:1261-8 pubmed publisher
    ..Whereas high-dose intravenous infections of mice with the rapidly growing mycobacterial species Mycobacterium smegmatis bearing an intact esx-3 locus were rapidly lethal, infection with an M...
  14. Altaf M, Miller C, Bellows D, O Toole R. Evaluation of the Mycobacterium smegmatis and BCG models for the discovery of Mycobacterium tuberculosis inhibitors. Tuberculosis (Edinb). 2010;90:333-7 pubmed publisher
    The objective of this study was to measure the efficacy of Mycobacterium smegmatis as a surrogate in vitro model for the detection of compounds which are inhibitory to the growth of Mycobacterium tuberculosis...
  15. Pawelczyk J, Brzostek A, Kremer L, Dziadek B, Rumijowska Galewicz A, Fiolka M, et al. AccD6, a key carboxyltransferase essential for mycolic acid synthesis in Mycobacterium tuberculosis, is dispensable in a nonpathogenic strain. J Bacteriol. 2011;193:6960-72 pubmed publisher
    ..tuberculosis, it can be deleted in Mycobacterium smegmatis without affecting its cell envelope integrity...
  16. Ordonez H, Shuman S. Mycobacterium smegmatis Lhr Is a DNA-dependent ATPase and a 3'-to-5' DNA translocase and helicase that prefers to unwind 3'-tailed RNA:DNA hybrids. J Biol Chem. 2013;288:14125-34 pubmed publisher
    ..Actinobacteria that includes the human pathogen Mycobacterium tuberculosis and its avirulent relative Mycobacterium smegmatis. Here, we identify and characterize M...
  17. Crellin P, Brammananth R, Coppel R. Decaprenylphosphoryl-?-D-ribose 2'-epimerase, the target of benzothiazinones and dinitrobenzamides, is an essential enzyme in Mycobacterium smegmatis. PLoS ONE. 2011;6:e16869 pubmed publisher
    ..Despite the exploitation of Mycobacterium smegmatis in the identification of DprE1 as the target of these new antimicrobials and its use in the exploration ..
  18. Dhiman R, Dinadayala P, Ryan G, Lenaerts A, Schenkel A, Crick D. Lipoarabinomannan localization and abundance during growth of Mycobacterium smegmatis. J Bacteriol. 2011;193:5802-9 pubmed publisher
    ..Analysis of Mycobacterium smegmatis subcellular fractions and spheroplasts showed that LAM and lipomannan (LM) were primarily found in a cell ..
  19. Li S, Kang J, Yu W, Zhou Y, Zhang W, Xin Y, et al. Identification of M. tuberculosis Rv3441c and M. smegmatis MSMEG_1556 and essentiality of M. smegmatis MSMEG_1556. PLoS ONE. 2012;7:e42769 pubmed publisher
    ..These results demonstrated that MSMEG_1556 is essential for growth of M. smegmatis. This study provided evidence that GlmM enzyme could be as a potential target for developing anti-tuberculosis drugs...
  20. Trauner A, Bennett M, Williams H. Isolation of bacterial ribosomes with monolith chromatography. PLoS ONE. 2011;6:e16273 pubmed publisher
    ..The speed and simplicity of this approach could accelerate the study of many different aspects of ribosomal biology. ..
  21. Rodrigues L, Ramos J, Couto I, Amaral L, Viveiros M. Ethidium bromide transport across Mycobacterium smegmatis cell-wall: correlation with antibiotic resistance. BMC Microbiol. 2011;11:35 pubmed publisher
    ..In this study, we have compared the Mycobacterium smegmatis wild-type strain mc2155 with knockout mutants for porins MspA (the main porin of M...
  22. England K, Crew R, Slayden R. Mycobacterium tuberculosis septum site determining protein, Ssd encoded by rv3660c, promotes filamentation and elicits an alternative metabolic and dormancy stress response. BMC Microbiol. 2011;11:79 pubmed publisher
  23. Taverniti V, Forti F, Ghisotti D, Putzer H. Mycobacterium smegmatis RNase J is a 5'-3' exo-/endoribonuclease and both RNase J and RNase E are involved in ribosomal RNA maturation. Mol Microbiol. 2011;82:1260-76 pubmed publisher
    ..Here, we identified a RNase J in Mycobacterium smegmatis that has both 5'-3' exo- and endonucleolytic activity...
  24. Behrends V, Williams K, Jenkins V, Robertson B, Bundy J. Free glucosylglycerate is a novel marker of nitrogen stress in Mycobacterium smegmatis. J Proteome Res. 2012;11:3888-96 pubmed publisher
    ..to monitor and quantify intracellular and extracellular metabolite levels (metabolic footprinting) in Mycobacterium smegmatis grown under nitrogen-limiting and nitrogen-rich conditions...
  25. Faludi I, Szabó A, Burian K, Endresz V, Miczak A. Recombinant Mycobacterium smegmatis vaccine candidates. Acta Microbiol Immunol Hung. 2011;58:13-22 pubmed publisher
    b>Mycobacterium smegmatis is a species of rapidly growing saprophytes with a number of properties that make it an effective vaccine vector. Recombinant M...
  26. Gupta R, Barkan D, Redelman Sidi G, Shuman S, Glickman M. Mycobacteria exploit three genetically distinct DNA double-strand break repair pathways. Mol Microbiol. 2011;79:316-30 pubmed publisher
    ..Here we demonstrate that Mycobacterium smegmatis has three DSB repair pathway options: HR, NHEJ and a novel mechanism of single-strand annealing (SSA)...
  27. Frampton R, Aggio R, Villas B as S, Arcus V, Cook G. Toxin-antitoxin systems of Mycobacterium smegmatis are essential for cell survival. J Biol Chem. 2012;287:5340-56 pubmed publisher
    ..b>Mycobacterium smegmatis contains three putative TA systems, VapBC, MazEF, and Phd/Doc, and previous work from our group has shown ..
  28. Kim K, An D, Song J, Yoon J, Kim H, Yoon H, et al. Mycobacterium tuberculosis Eis protein initiates suppression of host immune responses by acetylation of DUSP16/MKP-7. Proc Natl Acad Sci U S A. 2012;109:7729-34 pubmed publisher
    ..Enhanced intracellular survival (Eis) protein, secreted by Mtb, enhances survival of Mycobacterium smegmatis (Msm) in macrophages...
  29. Zhang R, Pan Y, He S, Lam M, Brayer G, Elbein A, et al. Mechanistic analysis of trehalose synthase from Mycobacterium smegmatis. J Biol Chem. 2011;286:35601-9 pubmed publisher
  30. Huang F, He Z. Characterization of a conserved interaction between DNA glycosylase and ParA in Mycobacterium smegmatis and M. tuberculosis. PLoS ONE. 2012;7:e38276 pubmed publisher
    ..and cell morphology by directly interacting with MsParA (Ms6939) and inhibiting its ATPase activity in Mycobacterium smegmatis. Using bacterial two-hybrid and pull-down techniques in combination with co-immunoprecipitation assays, ..
  31. Skovierova H, Larrouy Maumus G, Pham H, Belanova M, Barilone N, Dasgupta A, et al. Biosynthetic origin of the galactosamine substituent of Arabinogalactan in Mycobacterium tuberculosis. J Biol Chem. 2010;285:41348-55 pubmed publisher
    ..tuberculosis H37Rv. Conversely, expression of ppgS in Mycobacterium smegmatis conferred upon this species otherwise devoid of ppgS ortholog and any detectable polyprenyl-phospho-N-..
  32. García Pérez B, Villagómez Palatto D, Castañeda Sánchez J, Coral Vazquez R, Ramirez Sanchez I, Ordoñez Razo R, et al. Innate response of human endothelial cells infected with mycobacteria. Immunobiology. 2011;216:925-35 pubmed publisher
    ..As a whole, our results describe some aspects of the innate response of HUVEC infected by mycobacteria with different virulence and suggest that a strong cytoskeleton mobilization triggers a high NO production in these cells...
  33. Noens E, Williams C, Anandhakrishnan M, Poulsen C, Ehebauer M, Wilmanns M. Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1?C expression strain. BMC Biotechnol. 2011;11:27 pubmed publisher
    The non-pathogenic bacterium Mycobacterium smegmatis is widely used as a near-native expression host for the purification of Mycobacterium tuberculosis proteins...
  34. Sonawane A, Santos J, Mishra B, Jena P, Progida C, Sorensen O, et al. Cathelicidin is involved in the intracellular killing of mycobacteria in macrophages. Cell Microbiol. 2011;13:1601-17 pubmed publisher
    ..Here, we investigated in detail the antimycobacterial effect of murine and human cathelicidin against Mycobacterium smegmatis and M. bovis BCG infections...
  35. Shi T, Fu T, Xie J. Polyphosphate deficiency affects the sliding motility and biofilm formation of Mycobacterium smegmatis. Curr Microbiol. 2011;63:470-6 pubmed publisher
    ..This investigation provides novel recognition about the role of Rv1026, which provides novel clues for further study on the physiological role of Rv1026 in M. tuberculosis...
  36. McKenzie J, Robson J, Berney M, Smith T, Ruthe A, Gardner P, et al. A VapBC toxin-antitoxin module is a posttranscriptional regulator of metabolic flux in mycobacteria. J Bacteriol. 2012;194:2189-204 pubmed publisher
    ..Microarray analysis in Mycobacterium smegmatis overexpressing VapC/VapBC revealed a high percentage of downregulated genes with annotated roles in ..
  37. Raimunda D, Long J, Sassetti C, Arg ello J. Role in metal homeostasis of CtpD, a Co²? transporting P(1B4)-ATPase of Mycobacterium smegmatis. Mol Microbiol. 2012;84:1139-49 pubmed publisher
    ..Here we describe the functional role of the CtpD protein of Mycobacterium smegmatis. An M...
  38. Chen W, Green K, Tsodikov O, Garneau Tsodikova S. Aminoglycoside multiacetylating activity of the enhanced intracellular survival protein from Mycobacterium smegmatis and its inhibition. Biochemistry. 2012;51:4959-67 pubmed publisher
    The enhanced intracellular survival (Eis) protein improves the survival of Mycobacterium smegmatis (Msm) in macrophages and functions as the acetyltransferase responsible for kanamycin A resistance, a hallmark of extensively drug-..
  39. Zhang L, Li W, He Z. DarR, a TetR-like transcriptional factor, is a cyclic di-AMP-responsive repressor in Mycobacterium smegmatis. J Biol Chem. 2013;288:3085-96 pubmed publisher
    ..using a c-di-AMP/transcription factor binding screen, we identified Ms5346, a TetR family regulator in Mycobacterium smegmatis, as a c-di-AMP receptor in bacteria. Ms5346 could specifically bind c-di-AMP with K(d) of 2.3 ± 0.5 ?M...
  40. Mishra M, Daniels L. Characterization of the MSMEG_2631 gene (mmp) encoding a multidrug and toxic compound extrusion (MATE) family protein in Mycobacterium smegmatis and exploration of its polyspecific nature using biolog phenotype microarray. J Bacteriol. 2013;195:1610-21 pubmed publisher
    ..All together, our results show that M. smegmatis constitutively encodes an Na(+)-dependent MATE multidrug efflux pump from mmp in an operon with putative genes encoding proteins for apparently unrelated functions...
  41. Lee H, Lee N, Ko I, Kim S, Kang B, Oh J. Involvement of the catalytically important Asp54 residue of Mycobacterium smegmatis DevR in protein-protein interactions between DevR and DevS. FEMS Microbiol Lett. 2013;343:26-33 pubmed publisher
    The DevSR two-component system in Mycobacterium smegmatis consists of the DevS histidine kinase and the DevR response regulator. It is a regulatory system that is involved in the adaptation of mycobacteria to hypoxic and NO stresses...
  42. Jenkins V, Barton G, Robertson B, Williams K. Genome wide analysis of the complete GlnR nitrogen-response regulon in Mycobacterium smegmatis. BMC Genomics. 2013;14:301 pubmed publisher
    ..In the soil dwelling saprophyte Mycobacterium smegmatis the OmpR-type response regulator GlnR is thought to mediate the transcriptomic response to nitrogen ..
  43. Zhang L, He Z. Radiation-sensitive gene A (RadA) targets DisA, DNA integrity scanning protein A, to negatively affect cyclic Di-AMP synthesis activity in Mycobacterium smegmatis. J Biol Chem. 2013;288:22426-36 pubmed publisher
    ..we report a novel mechanism of control of c-di-AMP synthesis and its effects on bacterial growth in Mycobacterium smegmatis. We identified a DisA homolog in M. smegmatis, MsDisA, as an enzyme involved in c-di-AMP synthesis...
  44. Faisal S, Chen J, McDonough S, Chang C, Teng C, Chang Y. Immunostimulatory and antigen delivery properties of liposomes made up of total polar lipids from non-pathogenic bacteria leads to efficient induction of both innate and adaptive immune responses. Vaccine. 2011;29:2381-91 pubmed publisher
    ..Leptospira biflexa serovar Potac (designated leptosomes) and Mycobacterium smegmatis (designated smegmosomes) were evaluated for their adjuvant effects with various antigen presenting cells (..
  45. Jiang T, He L, Zhan Y, Zang S, Ma Y, Zhao X, et al. The effect of MSMEG_6402 gene disruption on the cell wall structure of Mycobacterium smegmatis. Microb Pathog. 2011;51:156-60 pubmed publisher
    ..However, there is no direct biochemical evidence since expression of Rv3807c has been unsuccessful. Mycobacterium smegmatis MSMEG_6402 is ortholog of Rv3807c...
  46. Yang Q, Liu Y, Huang F, He Z. Physical and functional interaction between D-ribokinase and topoisomerase I has opposite effects on their respective activity in Mycobacterium smegmatis and Mycobacterium tuberculosis. Arch Biochem Biophys. 2011;512:135-42 pubmed publisher
    ..tuberculosis and Mycobacterium smegmatis, respectively...
  47. Daugherty A, Powers K, Standley M, Kim C, Purdy G. Mycobacterium smegmatis RoxY is a repressor of oxyS and contributes to resistance to oxidative stress and bactericidal ubiquitin-derived peptides. J Bacteriol. 2011;193:6824-33 pubmed publisher
    ..We isolated 27 Mycobacterium smegmatis mutants that were hypersusceptible to Ub2...
  48. Iantomasi R, Sali M, Cascioferro A, Palucci I, Zumbo A, Soldini S, et al. PE_PGRS30 is required for the full virulence of Mycobacterium tuberculosis. Cell Microbiol. 2012;14:356-67 pubmed publisher
    ..Interestingly, when Mycobacterium smegmatis recombinant strain expressing PE_PGRS30 was used to infect macrophages or mice in vivo, we observed ..
  49. Leelaram M, Bhat A, Godbole A, Bhat R, Manjunath R, Nagaraja V. Type IA topoisomerase inhibition by clamp closure. FASEB J. 2013;27:3030-8 pubmed publisher
    ..We describe the mechanism of inhibition of Mycobacterium smegmatis and Mycobacterium tuberculosis topoI by monoclonal antibodies (mAbs) that bind with high affinity and ..
  50. Jiang T, Zhan Y, Sun M, Liu S, Zang S, Ma Y, et al. The novel responses of ethambutol against Mycobacterium smegmatis mc²155 Revealed by proteomics analysis. Curr Microbiol. 2011;62:341-5 pubmed publisher
    ..To elucidate the molecular mechanism of EMB against tuberculosis (TB), Mycobacterium smegmatis mc²155 was employed as a model of mycobacterial system in this study...
  51. Jin J, Zhang J, Guo N, Sheng H, Li L, Liang J, et al. Farnesol, a potential efflux pump inhibitor in Mycobacterium smegmatis. Molecules. 2010;15:7750-62 pubmed publisher
    ..both decreased the minimum inhibitory concentration (MIC) of ethidium bromide (EtBr) 8-fold against Mycobacterium smegmatis (M. smegmatis) mc²155 ATCC 700084 when incorporated at a concentration of 32 ?g/mL (FICI = 0...
  52. Uh a I, Gal n B, Morales V, Garc a J. Initial step in the catabolism of cholesterol by Mycobacterium smegmatis mc2 155. Environ Microbiol. 2011;13:943-59 pubmed publisher
    ..e. the transformation of cholesterol into cholestenone, has been investigated in Mycobacterium smegmatis. In silico analysis identified the MSMEG_1604 gene encoding a putative protein similar to the ChoD ..
  53. Yang M, Gao C, Cui T, An J, He Z. A TetR-like regulator broadly affects the expressions of diverse genes in Mycobacterium smegmatis. Nucleic Acids Res. 2012;40:1009-20 pubmed publisher
    Transcriptional regulation plays a critical role in the life cycle of Mycobacterium smegmatis and its related species, M. tuberculosis, the causative microbe for tuberculosis...
  54. Aldridge B, Fernandez Suarez M, Heller D, Ambravaneswaran V, Irimia D, Toner M, et al. Asymmetry and aging of mycobacterial cells lead to variable growth and antibiotic susceptibility. Science. 2012;335:100-4 pubmed publisher
    ..The physiologically distinct subpopulations of cells that arise through asymmetric growth and division are differentially susceptible to clinically important classes of antibiotics...
  55. Lamrabet O, Mba Medie F, Drancourt M. Acanthamoeba polyphaga-enhanced growth of Mycobacterium smegmatis. PLoS ONE. 2012;7:e29833 pubmed publisher
    b>Mycobacterium smegmatis is a rapidly-growing mycobacterium causing rare opportunistic infections in human patients. It is present in soil and water environments where free-living amoeba also reside, but data regarding M...
  56. Murdeshwar M, Chatterji D. MS_RHII-RSD, a dual-function RNase HII-(p)ppGpp synthetase from Mycobacterium smegmatis. J Bacteriol. 2012;194:4003-14 pubmed publisher
    In the noninfectious soil saprophyte Mycobacterium smegmatis, intracellular levels of the stress alarmones guanosine tetraphosphate and guanosine pentaphosphate, together termed (p)ppGpp, are regulated by the enzyme Rel(Msm)...
  57. Yakovleva L, Shuman S. Mycobacterium smegmatis SftH exemplifies a distinctive clade of superfamily II DNA-dependent ATPases with 3' to 5' translocase and helicase activities. Nucleic Acids Res. 2012;40:7465-75 pubmed publisher
    ..Actinobacteria, which includes the human pathogen Mycobacterium tuberculosis and its avirulent relative Mycobacterium smegmatis. Here, we identify and characterize M...
  58. Frenzel E, Schmidt S, Niederweis M, Steinhauer K. Importance of porins for biocide efficacy against Mycobacterium smegmatis. Appl Environ Microbiol. 2011;77:3068-73 pubmed publisher
    ..alamarBlue assay to show that the deletion of Msp porins in isogenic mutants increased the resistance of Mycobacterium smegmatis to isothiazolinones (methylchloroisothiazolinone [MCI]/methylisothiazolinone [MI] and ..
  59. Klepp L, Forrellad M, Osella A, Blanco F, Stella E, Bianco M, et al. Impact of the deletion of the six mce operons in Mycobacterium smegmatis. Microbes Infect. 2012;14:590-9 pubmed publisher
    The Mycobacterium smegmatis genome contains six operons designated mce (mammalian cell entry). These operons, which encode membrane and exported proteins, are highly conserved in pathogenic and non-pathogenic mycobacteria...
  60. Pelosi A, Smith D, Brammananth R, Topolska A, Billman Jacobe H, Nagley P, et al. Identification of a novel gene product that promotes survival of Mycobacterium smegmatis in macrophages. PLoS ONE. 2012;7:e31788 pubmed publisher
  61. Gröblacher B, Kunert O, Bucar F. Compounds of Alpinia katsumadai as potential efflux inhibitors in Mycobacterium smegmatis. Bioorg Med Chem. 2012;20:2701-6 pubmed publisher
    ..Although they showed weak antimycobacterial activities (MIC ? 64 mg/L), especially compound 1 revealed a significant activity on the EtBr accumulation and efflux as well as a synergistic effect in combination with rifampicin...
  62. Rich F, Kuhn S, Hyde E, Harper J, Ronchese F, Kirman J. Induction of T cell responses and recruitment of an inflammatory dendritic cell subset following tumor immunotherapy with Mycobacterium smegmatis. Cancer Immunol Immunother. 2012;61:2333-42 pubmed publisher
    ..Using a subcutaneous mouse thymoma model, we investigated whether immunotherapy with Mycobacterium smegmatis, a fast-growing mycobacterium of low pathogenicity, induces a systemic adaptive immune response...
  63. Roy S, Pahwa S, Nandanwar H, Jachak S. Phenylpropanoids of Alpinia galanga as efflux pump inhibitors in Mycobacterium smegmatis mc² 155. Fitoterapia. 2012;83:1248-55 pubmed publisher
    ..5, 6.25 and 5.0 mg/L respectively. 1'-S-1'-acetoxyeugenol acetate enhanced the accumulation and inhibited the efflux of EtBr in Mycobacterium smegmatis mc² 155 cells.
  64. Kushwaha A, Grove A. C-terminal low-complexity sequence repeats of Mycobacterium smegmatis Ku modulate DNA binding. Biosci Rep. 2013;33:175-84 pubmed publisher
    ..A unique feature of Mycobacterium smegmatis Ku is its basic C-terminal tail that contains several lysine-rich low-complexity PAKKA repeats that are ..
  65. Unciuleac M, Shuman S. Distinctive effects of domain deletions on the manganese-dependent DNA polymerase and DNA phosphorylase activities of Mycobacterium smegmatis polynucleotide phosphorylase. Biochemistry. 2013;52:2967-81 pubmed publisher
    ..Here we characterize and compare the RNA and DNA modifying activities of Mycobacterium smegmatis PNPase. The full-length (763-aa) M...
  66. Deshayes C, Bach H, Euphrasie D, Attarian R, Coureuil M, Sougakoff W, et al. MmpS4 promotes glycopeptidolipids biosynthesis and export in Mycobacterium smegmatis. Mol Microbiol. 2010;78:989-1003 pubmed publisher
    ..We showed by molecular genetics and biochemical analysis that MmpS4 in Mycobacterium smegmatis is required for the production and export of large amounts of cell surface glycolipids, but is ..
  67. Gupta K, Kumar P, Chatterji D. Identification, activity and disulfide connectivity of C-di-GMP regulating proteins in Mycobacterium tuberculosis. PLoS ONE. 2010;5:e15072 pubmed publisher
    ..Previously, we reported a bifunctional GGDEF-EAL domain protein, MSDGC-1 from Mycobacterium smegmatis showing both these activities (Kumar and Chatterji, 2008)...
  68. Crespo E, Cristescu S, de Ronde H, Kuijper S, Kolk A, Anthony R, et al. Proton Transfer Reaction Mass Spectrometry detects rapid changes in volatile metabolite emission by Mycobacterium smegmatis after the addition of specific antimicrobial agents. J Microbiol Methods. 2011;86:8-15 pubmed publisher
    ..headspace of actively growing cultures of paired ciprofloxacin sensitive and resistant bacterial strains (Mycobacterium smegmatis in Middlebrook M7H9 liquid media) after the addition of the antibiotics ciprofloxacin and gentamicin in ..
  69. Uhía I, Galán B, Medrano F, Garcia J. Characterization of the KstR-dependent promoter of the gene for the first step of the cholesterol degradative pathway in Mycobacterium smegmatis. Microbiology. 2011;157:2670-80 pubmed publisher
    The KstR-dependent promoter of the MSMEG_5228 gene of Mycobacterium smegmatis, which encodes the 3-?-hydroxysteroid dehydrogenase (3-? HSD(MS)) responsible for the first step in the cholesterol degradative pathway, has been characterized...
  70. Ponaire S, Zinglé C, Tritsch D, Grosdemange Billiard C, Rohmer M. Growth inhibition of Mycobacterium smegmatis by prodrugs of deoxyxylulose phosphate reducto-isomerase inhibitors, promising anti-mycobacterial agents. Eur J Med Chem. 2012;51:277-85 pubmed publisher
    ..acyloxymethyl phosphonate esters as prodrugs of fosmidomycin 1a, FR900098 1b and their analogs 2a and 2b on Mycobacterium smegmatis. Only the prodrugs 4b-6b inhibit the bacterial growth and could be effective anti-mycobacterial agents.
  71. Zhu H, Bhattarai H, Yan H, Shuman S, Glickman M. Characterization of Mycobacterium smegmatis PolD2 and PolD1 as RNA/DNA polymerases homologous to the POL domain of bacterial DNA ligase D. Biochemistry. 2012;51:10147-58 pubmed publisher
    ..Deletion of polD1, polD2, or both from a Mycobacterium smegmatis strain carrying an inactivating mutation in LigD POL failed to reveal a role for PolD1 or PolD2 in ..
  72. Williams K, Bennett M, Barton G, Jenkins V, Robertson B. Adenylylation of mycobacterial Glnk (PII) protein is induced by nitrogen limitation. Tuberculosis (Edinb). 2013;93:198-206 pubmed publisher
    ..mycobacterial GlnK (PII), and demonstrate that during nitrogen limitation GlnK from both non-pathogenic Mycobacterium smegmatis and pathogenic Mycobacterium tuberculosis is adenylylated at the Tyrosine-51 residue; we also show that ..
  73. Jones C, Niederweis M. Role of porins in iron uptake by Mycobacterium smegmatis. J Bacteriol. 2010;192:6411-7 pubmed publisher
    ..The outer membrane of the saprophytic Mycobacterium smegmatis contains the Msp family of porins, which enable the diffusion of small and hydrophilic solutes, such as ..
  74. Jin J, Zhang J, Guo N, Feng H, Li L, Liang J, et al. The plant alkaloid piperine as a potential inhibitor of ethidium bromide efflux in Mycobacterium smegmatis. J Med Microbiol. 2011;60:223-9 pubmed publisher
    ..bromide (EtBr) twofold at 32 ?g ml(-1) and fourfold at 64 ?g ml(-1) against Mycobacterium smegmatis mc(2) 155 ATCC 700084...
  75. Dhouib R, Ducret A, Hubert P, Carriere F, Dukan S, Canaan S. Watching intracellular lipolysis in mycobacteria using time lapse fluorescence microscopy. Biochim Biophys Acta. 2011;1811:234-41 pubmed publisher
    ..It should be particularly useful for studying the effects of chemical inhibitors and activators on cells as well as investigating other metabolic pathways...
  76. Hawkins B, Huang C, Arasanipalai S, Kirby B. Automated dielectrophoretic characterization of Mycobacterium smegmatis. Anal Chem. 2011;83:3507-15 pubmed publisher
    We report the positive dielectrophoretic (pDEP) characterization of wild-type and ethambutol-treated Mycobacterium smegmatis populations via automated pDEP cell trapping experiments...
  77. Jenkins V, Robertson B, Williams K. Aspartate D48 is essential for the GlnR-mediated transcriptional response to nitrogen limitation in Mycobacterium smegmatis. FEMS Microbiol Lett. 2012;330:38-45 pubmed publisher
    ..The transcriptional response to nitrogen limitation in Mycobacterium smegmatis is thought to be regulated by GlnR, although, to date, only five nitrogen metabolism genes have been ..
  78. Brzostek A, Rumijowska Galewicz A, Dziadek B, Wojcik E, Dziadek J. ChoD and HsdD can be dispensable for cholesterol degradation in mycobacteria. J Steroid Biochem Mol Biol. 2013;134:1-7 pubmed publisher
    ..and directly show that both ChoD and HsdD are dispensable for cholesterol degradation in fast-growing Mycobacterium smegmatis mc(2)155 and slow-growing Mycobacterium tuberculosis H37Rv strains...
  79. Li S, Ng P, Qin H, Lau J, Lau J, Tsui S, et al. Identification of small RNAs in Mycobacterium smegmatis using heterologous Hfq. RNA. 2013;19:74-84 pubmed publisher
    ..inherent sRNAs-binding capability of heterologous Hfq from Escherichia coli to enrich sRNAs from Mycobacterium smegmatis, a model organism for studying Mycobacterium tuberculosis...
  80. Li H, Jogl G. Crystal structure of decaprenylphosphoryl-?- D-ribose 2'-epimerase from Mycobacterium smegmatis. Proteins. 2013;81:538-43 pubmed publisher
    ..We determined the crystal structure of a truncated form of DprE1 from Mycobacterium smegmatis in two crystal forms to up to 2.35 Å resolution...
  81. Unciuleac M, Shuman S. Double strand break unwinding and resection by the mycobacterial helicase-nuclease AdnAB in the presence of single strand DNA-binding protein (SSB). J Biol Chem. 2010;285:34319-29 pubmed publisher
    ..These results extend our understanding of presynaptic DSB processing by AdnAB and engender instructive comparisons with the RecBCD and AddAB clades of bacterial helicase-nuclease machines...
  82. Zhang H, Peng P, Miao S, Zhao Y, Mao F, Wang L, et al. Recombinant Mycobacterium smegmatis expressing an ESAT6-CFP10 fusion protein induces anti-mycobacterial immune responses and protects against Mycobacterium tuberculosis challenge in mice. Scand J Immunol. 2010;72:349-57 pubmed publisher
    ..In this study, we evaluated a recombinant vaccine prepared from non-pathogenic Mycobacterium smegmatis (rMS) that expresses a fusion of early secreted antigenic target 6-kDa antigen (ESAT6) and culture ..
  83. Whiteford D, Klingelhoets J, Bambenek M, Dahl J. Deletion of the histone-like protein (Hlp) from Mycobacterium smegmatis results in increased sensitivity to UV exposure, freezing and isoniazid. Microbiology. 2011;157:327-35 pubmed publisher
    ..As a soil-dwelling saprophyte, Mycobacterium smegmatis is exposed to factors such as UV light and rounds of freezing and thawing that occur in temperate ..
  84. Torrelles J, Sieling P, Arcos J, Knaup R, Bartling C, Rajaram M, et al. Structural differences in lipomannans from pathogenic and nonpathogenic mycobacteria that impact CD1b-restricted T cell responses. J Biol Chem. 2011;286:35438-46 pubmed publisher
    ..Thus, here we correlate the structure-function relationships for LMs with CD1b-restricted T cell responses and provide evidence that the structural features of TB-LM contribute to its diminished T cell responsiveness...
  85. Chavadi S, Stirrett K, Edupuganti U, Vergnolle O, Sadhanandan G, Marchiano E, et al. Mutational and phylogenetic analyses of the mycobacterial mbt gene cluster. J Bacteriol. 2011;193:5905-13 pubmed publisher
    ..We report a systematic mutational analysis of the mbt gene cluster ortholog found in Mycobacterium smegmatis. This mutational analysis demonstrates that eight of the nine mbt genes investigated are essential for ..
  86. Zullo A, Lee S. Mycobacterial induction of autophagy varies by species and occurs independently of mammalian target of rapamycin inhibition. J Biol Chem. 2012;287:12668-78 pubmed publisher
    ..Analysis of Mycobacterium smegmatis and Mycobacterium bovis bacille Calmette-Guérin (BCG), which respectively induce high and low autophagy ..
  87. Plocinska R, Purushotham G, Sarva K, Vadrevu I, Pandeeti E, Arora N, et al. Septal localization of the Mycobacterium tuberculosis MtrB sensor kinase promotes MtrA regulon expression. J Biol Chem. 2012;287:23887-99 pubmed publisher
    ..fusion protein localized to the cell membrane, the septa, and the poles in Mycobacterium tuberculosis and Mycobacterium smegmatis. This localization was independent of MtrB phosphorylation status but dependent upon the assembly of FtsZ,..
  88. Ginda K, Bezulska M, Zi kiewicz M, Dziadek J, Zakrzewska Czerwi ska J, Jakimowicz D. ParA of Mycobacterium smegmatis co-ordinates chromosome segregation with the cell cycle and interacts with the polar growth determinant DivIVA. Mol Microbiol. 2013;87:998-1012 pubmed publisher
    ..Further research has demonstrated that a Mycobacterium smegmatis parB deletion mutant was viable but exhibited a chromosome segregation defect...
  89. Sao Emani C, Williams M, Wiid I, Hiten N, Viljoen A, Pietersen R, et al. Ergothioneine is a secreted antioxidant in Mycobacterium smegmatis. Antimicrob Agents Chemother. 2013;57:3202-7 pubmed publisher
    ..In this study, quantification of ERG at various points in the growth cycle of Mycobacterium smegmatis revealed that a significant portion of ERG is found in the culture media, suggesting that it is actively ..
  90. Alderwick L, Lloyd G, Ghadbane H, May J, Bhatt A, Eggeling L, et al. The C-terminal domain of the Arabinosyltransferase Mycobacterium tuberculosis EmbC is a lectin-like carbohydrate binding module. PLoS Pathog. 2011;7:e1001299 pubmed publisher
    ..This work provides the first step towards unravelling the structure and function of a GT-C-type glycosyltransferase that is essential in M. tuberculosis...
  91. Bharati B, Sharma I, Kasetty S, Kumar M, Mukherjee R, Chatterji D. A full-length bifunctional protein involved in c-di-GMP turnover is required for long-term survival under nutrient starvation in Mycobacterium smegmatis. Microbiology. 2012;158:1415-27 pubmed publisher
    ..MSDGC-1 (an orthologue of Rv1354c in Mycobacterium tuberculosis), involved in c-di-GMP turnover in Mycobacterium smegmatis. MSDGC-1 is a multidomain protein, having GAF, GGDEF and EAL domains arranged in tandem, and exhibits ..
  92. Fukuda T, Matsumura T, Ato M, Hamasaki M, Nishiuchi Y, Murakami Y, et al. Critical roles for lipomannan and lipoarabinomannan in cell wall integrity of mycobacteria and pathogenesis of tuberculosis. MBio. 2013;4:e00472-12 pubmed publisher
    ..In Mycobacterium smegmatis, structural defects in LM and LAM resulted in loss of acid-fast staining, increased sensitivity to ?-..