corynebacterium glutamicum

Summary

Summary: A species of gram-positive, asporogenous, non-pathogenic, soil bacteria that produces GLUTAMIC ACID.

Top Publications

  1. Rehm N, Burkovski A. Engineering of nitrogen metabolism and its regulation in Corynebacterium glutamicum: influence on amino acid pools and production. Appl Microbiol Biotechnol. 2011;89:239-48 pubmed publisher
    ..This mini-review focuses on nitrogen metabolism and its control in the biotechnology workhorse Corynebacterium glutamicum, which is used for the industrial production of more than 2 million tons of L: -amino acids annually...
  2. Yao W, Chu C, Deng X, Zhang Y, Liu M, Zheng P, et al. Display of alpha-amylase on the surface of Corynebacterium glutamicum cells by using NCgl1221 as the anchoring protein, and production of glutamate from starch. Arch Microbiol. 2009;191:751-9 pubmed publisher
    We developed a new cell surface display system in Corynebacterium glutamicum based on the C-terminally truncated NCgl1221 anchor protein to increase L-glutamate production from starch directly...
  3. Lee S, Shin H, Park J, Kim Y, Min J. Proline reduces the binding of transcriptional regulator ArgR to upstream of argB in Corynebacterium glutamicum. Appl Microbiol Biotechnol. 2010;86:235-42 pubmed publisher
  4. Bansal Mutalik R, Nikaido H. Quantitative lipid composition of cell envelopes of Corynebacterium glutamicum elucidated through reverse micelle extraction. Proc Natl Acad Sci U S A. 2011;108:15360-5 pubmed publisher
    ..Here we use the extraction, with reverse surfactant micelles, of intact cells of Corynebacterium glutamicum and show that this method extracts the free OM lipids quantitatively with no contamination from lipids ..
  5. Hashimoto K, Murata J, Konishi T, Yabe I, Nakamatsu T, Kawasaki H. Glutamate is excreted across the cytoplasmic membrane through the NCgl1221 channel of Corynebacterium glutamicum by passive diffusion. Biosci Biotechnol Biochem. 2012;76:1422-4 pubmed
    ..channel, has been reported to be critically involved in glutamate (Glu) overproduction by Corynebacterium glutamicum, but direct evidence of Glu excretion through this channel has not yet been provided...
  6. Sasaki M, Jojima T, Inui M, Yukawa H. Xylitol production by recombinant Corynebacterium glutamicum under oxygen deprivation. Appl Microbiol Biotechnol. 2010;86:1057-66 pubmed publisher
    Wild-type Corynebacterium glutamicum produced 0.6 g l(-1) xylitol from xylose at a productivity of 0.01 g l(-1) h(-1) under oxygen deprivation. To increase this productivity, the pentose transporter gene (araE) from C...
  7. Bolten C, Schröder H, Dickschat J, Wittmann C. Towards methionine overproduction in Corynebacterium glutamicum--methanethiol and dimethyldisulfide as reduced sulfur sources. J Microbiol Biotechnol. 2010;20:1196-203 pubmed
    ..work, methanethiol and dimethyldisulfide were investigated as sulfur source for methionine synthesis in Corynebacterium glutamicum. In silico pathway analysis has predicted a high methionine yield for these reduced compounds provided ..
  8. Sharkey M, Maher M, Guyonvarch A, Engel P. Kinetic characterisation of recombinant Corynebacterium glutamicum NAD+-dependent LDH over-expressed in E. coli and its rescue of an lldD- phenotype in C. glutamicum: the issue of reversibility re-examined. Arch Microbiol. 2011;193:731-40 pubmed publisher
    The ldh gene of Corynebacterium glutamicum ATCC 13032 (gene symbol cg3219, encoding a 314 residue NAD+-dependent L-(+)-lactate dehydrogenase, EC 1.1.1.27) was cloned into the expression vector pKK388-1 and over-expressed in an ldhA-null E...
  9. Neumeyer A, Hübschmann T, Müller S, Frunzke J. Monitoring of population dynamics of Corynebacterium glutamicum by multiparameter flow cytometry. Microb Biotechnol. 2013;6:157-67 pubmed publisher
    ..fluorescent dyes for suitability in FC single cell analysis of the biotechnological platform organism Corynebacterium glutamicum. Already simple scattering properties of C...

More Information

Publications160 found, 100 shown here

  1. Rumbold K, van Buijsen H, Gray V, van Groenestijn J, Overkamp K, Slomp R, et al. Microbial renewable feedstock utilization: a substrate-oriented approach. Bioeng Bugs. 2010;1:359-66 pubmed publisher
    ..The performance of six industrially relevant microorganisms, i.e., two bacteria (Escherichia coli and Corynebacterium glutamicum), two yeasts (Saccharomyces cerevisiae and Pichia stipitis) and two fungi (Aspergillus niger and ..
  2. Chimponda T, Mukanganyama S. Antimycobacterial activities of selected medicinal plants from Zimbabwe against Mycobacterium aurum and Corynebacterium glutamicum. Trop Biomed. 2010;27:595-610 pubmed
    ..extracts from nineteen selected plants from Zimbabwe were screened against Mycobacterium aurum and Corynebacterium glutamicum using the agar disk diffusion method...
  3. Takahashi C, Shirakawa J, Tsuchidate T, Okai N, Hatada K, Nakayama H, et al. Robust production of gamma-amino butyric acid using recombinant Corynebacterium glutamicum expressing glutamate decarboxylase from Escherichia coli. Enzyme Microb Technol. 2012;51:171-6 pubmed publisher
    ..Here, we report a simple and robust system to produce GABA from glucose using the recombinant Corynebacterium glutamicum strain GAD, which expresses GadB, a glutamate decarboxylase encoded by the gadB gene of Escherichia coli ..
  4. Becker J, Klopprogge C, Schröder H, Wittmann C. Metabolic engineering of the tricarboxylic acid cycle for improved lysine production by Corynebacterium glutamicum. Appl Environ Microbiol. 2009;75:7866-9 pubmed publisher
    In the present work, lysine production by Corynebacterium glutamicum was improved by metabolic engineering of the tricarboxylic acid (TCA) cycle...
  5. Tsuge Y, Yamamoto S, Suda M, Inui M, Yukawa H. Reactions upstream of glycerate-1,3-bisphosphate drive Corynebacterium glutamicum (D)-lactate productivity under oxygen deprivation. Appl Microbiol Biotechnol. 2013;97:6693-703 pubmed publisher
    We previously demonstrated the simplicity of oxygen-deprived Corynebacterium glutamicum to produce D-lactate, a primary building block of next-generation biodegradable plastics, at very high optical purity by introducing heterologous D-..
  6. Auchter M, Cramer A, Hüser A, Ruckert C, Emer D, Schwarz P, et al. RamA and RamB are global transcriptional regulators in Corynebacterium glutamicum and control genes for enzymes of the central metabolism. J Biotechnol. 2011;154:126-39 pubmed publisher
    In Corynebacterium glutamicum, the transcriptional regulators of acetate metabolism RamA (encoded by cg2831) and RamB (encoded by cg0444) play an important role in expression control of genes involved in acetate and ethanol metabolism...
  7. Nakayama Y, Yoshimura K, Iida H. Electrophysiological characterization of the mechanosensitive channel MscCG in Corynebacterium glutamicum. Biophys J. 2013;105:1366-75 pubmed publisher
    b>Corynebacterium glutamicum MscCG, also referred to as NCgl1221, exports glutamate when biotin is limited in the culture medium. MscCG is a homolog of Escherichia coli MscS, which serves as an osmotic safety valve in E. coli cells...
  8. Frunzke J, Gätgens C, Brocker M, Bott M. Control of heme homeostasis in Corynebacterium glutamicum by the two-component system HrrSA. J Bacteriol. 2011;193:1212-21 pubmed publisher
    ..named CgtSR11) was recently found to be repressed by the global iron-dependent regulator DtxR in Corynebacterium glutamicum. Here, we provide evidence that HrrA mediates heme-dependent gene regulation in this nonpathogenic soil ..
  9. Li M, Li D, Huang Y, Liu M, Wang H, Tang Q, et al. Improving the secretion of cadaverine in Corynebacterium glutamicum by cadaverine-lysine antiporter. J Ind Microbiol Biotechnol. 2014;41:701-9 pubmed publisher
    ..Biosynthesis of cadaverine in Corynebacterium glutamicum has been a competitive way in place of petroleum-based chemical synthesis method...
  10. Tanaka Y, Teramoto H, Inui M, Yukawa H. Translation efficiency of antiterminator proteins is a determinant for the difference in glucose repression of two ?-glucoside phosphotransferase system gene clusters in Corynebacterium glutamicum R. J Bacteriol. 2011;193:349-57 pubmed publisher
    b>Corynebacterium glutamicum R has two ?-glucoside phosphoenolpyruvate, carbohydrate phosphotransferase systems (PTS) encoded by bglF and bglF2 located in the respective clusters, bglF-bglA-bglG and bglF2-bglA2-bglG2...
  11. Toyoda K, Teramoto H, Gunji W, Inui M, Yukawa H. Involvement of regulatory interactions among global regulators GlxR, SugR, and RamA in expression of ramA in Corynebacterium glutamicum. J Bacteriol. 2013;195:1718-26 pubmed publisher
    The central carbon metabolism genes in Corynebacterium glutamicum are under the control of a transcriptional regulatory network composed of several global regulators...
  12. Park J, Lee S. Metabolic pathways and fermentative production of L-aspartate family amino acids. Biotechnol J. 2010;5:560-77 pubmed publisher
    ..Finally, perspectives of systems metabolic engineering for developing AFAA overproducers are suggested with selected exemplary studies. ..
  13. Mishra A, Krumbach K, Rittmann D, Appelmelk B, Pathak V, Pathak A, et al. Lipoarabinomannan biosynthesis in Corynebacterineae: the interplay of two ?(1?2)-mannopyranosyltransferases MptC and MptD in mannan branching. Mol Microbiol. 2011;80:1241-59 pubmed publisher
    ..Herein, we have identified and characterized two open reading frames from Corynebacterium glutamicum that encode for putative GT-Cs. Deletion of NCgl2100 and NCgl2097 in C...
  14. Schneider J, Peters Wendisch P, Stansen K, Götker S, Maximow S, Kramer R, et al. Characterization of the biotin uptake system encoded by the biotin-inducible bioYMN operon of Corynebacterium glutamicum. BMC Microbiol. 2012;12:6 pubmed publisher
    The amino acid-producing Gram-positive Corynebacterium glutamicum is auxotrophic for biotin although biotin ring assembly starting from the precursor pimeloyl-CoA is still functional...
  15. Vertès A, Inui M, Yukawa H. Postgenomic approaches to using corynebacteria as biocatalysts. Annu Rev Microbiol. 2012;66:521-50 pubmed publisher
    b>Corynebacterium glutamicum exhibits numerous ideal intrinsic attributes as a factory of primary and secondary metabolites...
  16. Wieschalka S, Blombach B, Bott M, Eikmanns B. Bio-based production of organic acids with Corynebacterium glutamicum. Microb Biotechnol. 2013;6:87-102 pubmed publisher
    ..b>Corynebacterium glutamicum is well known as workhorse for the industrial production of numerous amino acids...
  17. Ikeda M, Miyamoto A, Mutoh S, Kitano Y, Tajima M, Shirakura D, et al. Development of biotin-prototrophic and -hyperauxotrophic Corynebacterium glutamicum strains. Appl Environ Microbiol. 2013;79:4586-94 pubmed publisher
    To develop the infrastructure for biotin production through naturally biotin-auxotrophic Corynebacterium glutamicum, we attempted to engineer the organism into a biotin prototroph and a biotin hyperauxotroph...
  18. Wu H, Li Q, Lu R, Wang Y, Zhuang X, He N. Fed-batch production of a bioflocculant from Corynebacterium glutamicum. J Ind Microbiol Biotechnol. 2010;37:1203-9 pubmed publisher
    ..were obtained by feeding with a sucrose-urea solution in a pilot scale fermentation process, indicating the potential industrial utility of this constant-rate feeding strategy in bioflocculant production by Corynebacterium glutamicum.
  19. Maeda T, Wachi M. 3' Untranslated region-dependent degradation of the aceA mRNA, encoding the glyoxylate cycle enzyme isocitrate lyase, by RNase E/G in Corynebacterium glutamicum. Appl Environ Microbiol. 2012;78:8753-61 pubmed publisher
    We previously reported that the Corynebacterium glutamicum RNase E/G encoded by the rneG gene (NCgl2281) is required for the 5' maturation of 5S rRNA...
  20. Buchholz J, Schwentner A, Brunnenkan B, Gabris C, Grimm S, Gerstmeir R, et al. Platform engineering of Corynebacterium glutamicum with reduced pyruvate dehydrogenase complex activity for improved production of L-lysine, L-valine, and 2-ketoisovalerate. Appl Environ Microbiol. 2013;79:5566-75 pubmed publisher
    Exchange of the native Corynebacterium glutamicum promoter of the aceE gene, encoding the E1p subunit of the pyruvate dehydrogenase complex (PDHC), with mutated dapA promoter variants led to a series of C...
  21. Park J, Lee J, Kim H, Kim E, Kim P, Kim Y, et al. The role of Corynebacterium glutamicum spiA gene in whcA-mediated oxidative stress gene regulation. FEMS Microbiol Lett. 2012;331:63-9 pubmed publisher
    The Corynebacterium glutamicum WhcA protein, which inhibits the expression of oxidative stress response genes, is known to interact with the SpiA protein...
  22. Lanéelle M, Tropis M, Daffe M. Current knowledge on mycolic acids in Corynebacterium glutamicum and their relevance for biotechnological processes. Appl Microbiol Biotechnol. 2013;97:9923-30 pubmed publisher
    b>Corynebacterium glutamicum is the world's largest producer of glutamate and lysine...
  23. Bussmann M, Emer D, Hasenbein S, Degraf S, Eikmanns B, Bott M. Transcriptional control of the succinate dehydrogenase operon sdhCAB of Corynebacterium glutamicum by the cAMP-dependent regulator GlxR and the LuxR-type regulator RamA. J Biotechnol. 2009;143:173-82 pubmed publisher
    In experiments performed to identify transcriptional regulators of the tricarboxylic acid cycle of Corynebacterium glutamicum, the cAMP-dependent regulator GlxR and the regulators of acetate metabolism RamA and RamB were enriched by DNA ..
  24. Kohl T, Tauch A. The GlxR regulon of the amino acid producer Corynebacterium glutamicum: Detection of the corynebacterial core regulon and integration into the transcriptional regulatory network model. J Biotechnol. 2009;143:239-46 pubmed publisher
    b>Corynebacterium glutamicum is an industrially important producer of amino acids and an emerging model system for the Corynebacterineae. The glxR gene of C...
  25. Litsanov B, Kabus A, Brocker M, Bott M. Efficient aerobic succinate production from glucose in minimal medium with Corynebacterium glutamicum. Microb Biotechnol. 2012;5:116-28 pubmed publisher
    b>Corynebacterium glutamicum, an established industrial amino acid producer, has been genetically modified for efficient succinate production from the renewable carbon source glucose under fully aerobic conditions in minimal medium...
  26. Kim I, Jeong W, Lim S, Hwang I, Kim Y. The small ribosomal protein S12P gene rpsL as an efficient positive selection marker in allelic exchange mutation systems for Corynebacterium glutamicum. J Microbiol Methods. 2011;84:128-30 pubmed publisher
    We report that the mutant rpsL K43R in streptomycin-resistant and lysine-producing Corynebacterium glutamicum is responsible for streptomycin resistance. In addition, we describe its effective application in gene modification in C...
  27. Lai S, Zhang Y, Liu S, Liang Y, Shang X, Chai X, et al. Metabolic engineering and flux analysis of Corynebacterium glutamicum for L-serine production. Sci China Life Sci. 2012;55:283-90 pubmed publisher
    ..In this study, Corynebacterium glutamicum ATCC 13032 was engineered de novo by blocking and attenuating the conversion of L-serine to pyruvate and ..
  28. Scheele S, Oertel D, Bongaerts J, Evers S, Hellmuth H, Maurer K, et al. Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol-xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum. Microb Biotechnol. 2013;6:202-6 pubmed publisher
    ..Using the industrial workhorse Corynebacterium glutamicum as the expression host, successful secretion of a normally cytosolic FAD cofactor-containing sorbitol-..
  29. Schneider J, Niermann K, Wendisch V. Production of the amino acids l-glutamate, l-lysine, l-ornithine and l-arginine from arabinose by recombinant Corynebacterium glutamicum. J Biotechnol. 2011;154:191-8 pubmed publisher
    Amino acid production processes with Corynebacterium glutamicum are based on media containing glucose from starch hydrolysis or fructose and sucrose as present in molasses...
  30. Ikeda M, Mizuno Y, Awane S, Hayashi M, Mitsuhashi S, Takeno S. Identification and application of a different glucose uptake system that functions as an alternative to the phosphotransferase system in Corynebacterium glutamicum. Appl Microbiol Biotechnol. 2011;90:1443-51 pubmed publisher
    b>Corynebacterium glutamicum uses the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) to uptake and phosphorylate glucose; no other route has yet been identified. Disruption of the ptsH gene in wild-type C...
  31. Binder S, Schendzielorz G, Stäbler N, Krumbach K, Hoffmann K, Bott M, et al. A high-throughput approach to identify genomic variants of bacterial metabolite producers at the single-cell level. Genome Biol. 2012;13:R40 pubmed publisher
    ..for visualizing intracellular metabolite concentrations within single cells of Escherichia coli and Corynebacterium glutamicum that expedites the screening process of producers...
  32. Nishimura T, Teramoto H, Toyoda K, Inui M, Yukawa H. Regulation of the nitrate reductase operon narKGHJI by the cAMP-dependent regulator GlxR in Corynebacterium glutamicum. Microbiology. 2011;157:21-8 pubmed publisher
    The Corynebacterium glutamicum anaerobic nitrate reductase operon narKGHJI is repressed by a transcriptional regulator, ArnR, under aerobic conditions...
  33. Jojima T, Fujii M, Mori E, Inui M, Yukawa H. Engineering of sugar metabolism of Corynebacterium glutamicum for production of amino acid L-alanine under oxygen deprivation. Appl Microbiol Biotechnol. 2010;87:159-65 pubmed publisher
    b>Corynebacterium glutamicum was genetically engineered to produce L-alanine from sugar under oxygen deprivation. The genes associated with production of organic acids in C...
  34. Kan S, Liu J, Hu H, Chang C, Lin W, Wang W, et al. Biochemical characterization of two thymidylate synthases in Corynebacterium glutamicum NCHU 87078. Biochim Biophys Acta. 2010;1804:1751-9 pubmed publisher
    The genome of Corynebacterium glutamicum NCHU 87078 contains two putative thymidylate synthase genes, designated CgthyA and CgthyX...
  35. Siedler S, Lindner S, Bringer S, Wendisch V, Bott M. Reductive whole-cell biotransformation with Corynebacterium glutamicum: improvement of NADPH generation from glucose by a cyclized pentose phosphate pathway using pfkA and gapA deletion mutants. Appl Microbiol Biotechnol. 2013;97:143-52 pubmed publisher
    In this study, the potential of Corynebacterium glutamicum for reductive whole-cell biotransformation is shown...
  36. Uhde A, Youn J, Maeda T, Clermont L, Matano C, Kramer R, et al. Glucosamine as carbon source for amino acid-producing Corynebacterium glutamicum. Appl Microbiol Biotechnol. 2013;97:1679-87 pubmed publisher
    b>Corynebacterium glutamicum grows with a variety of carbohydrates and carbohydrate derivatives as sole carbon sources; however, growth with glucosamine has not yet been reported...
  37. Schultz C, Niebisch A, Schwaiger A, Viets U, Metzger S, Bramkamp M, et al. Genetic and biochemical analysis of the serine/threonine protein kinases PknA, PknB, PknG and PknL of Corynebacterium glutamicum: evidence for non-essentiality and for phosphorylation of OdhI and FtsZ by multiple kinases. Mol Microbiol. 2009;74:724-41 pubmed publisher
    We previously showed that the 2-oxoglutarate dehydrogenase inhibitor protein OdhI of Corynebacterium glutamicum is phosphorylated by PknG at Thr14, but that also additional serine/threonine protein kinases (STPKs) can phosphorylate OdhI...
  38. Mustafi N, Grünberger A, Kohlheyer D, Bott M, Frunzke J. The development and application of a single-cell biosensor for the detection of l-methionine and branched-chain amino acids. Metab Eng. 2012;14:449-57 pubmed publisher
    ..transcriptional regulator Lrp that detects intracellular l-methionine and branched-chain amino acids in Corynebacterium glutamicum. In assays, fluorescence output showed a linear relationship with cytoplasmic concentrations of the ..
  39. Nishimura T, Teramoto H, Inui M, Yukawa H. Gene expression profiling of Corynebacterium glutamicum during Anaerobic nitrate respiration: induction of the SOS response for cell survival. J Bacteriol. 2011;193:1327-33 pubmed publisher
    The gene expression profile of Corynebacterium glutamicum under anaerobic nitrate respiration revealed marked differences in the expression levels of a number of genes involved in a variety of cellular functions, including carbon ..
  40. Busche T, Silar R, Pičmanová M, Pátek M, Kalinowski J. Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum. BMC Genomics. 2012;13:445 pubmed publisher
    The expression of genes in Corynebacterium glutamicum, a Gram-positive non-pathogenic bacterium used mainly for the industrial production of amino acids, is regulated by seven different sigma factors of RNA polymerase, including the ..
  41. Ochrombel I, Ott L, Kramer R, Burkovski A, Marin K. Impact of improved potassium accumulation on pH homeostasis, membrane potential adjustment and survival of Corynebacterium glutamicum. Biochim Biophys Acta. 2011;1807:444-50 pubmed publisher
    ..By applying Corynebacterium glutamicum as model system we provide experimental evidence that under optimal conditions a potassium channel is ..
  42. An S, Yim S, Jeong K. Development of a secretion system for the production of heterologous proteins in Corynebacterium glutamicum using the Porin B signal peptide. Protein Expr Purif. 2013;89:251-7 pubmed publisher
    b>Corynebacterium glutamicum is one of the useful hosts for the secretory production of heterologous proteins because of intrinsic attributes such as the presence of few endogenous proteins and proteases in culture medium...
  43. Fränzel B, Trötschel C, Ruckert C, Kalinowski J, Poetsch A, Wolters D. Adaptation of Corynebacterium glutamicum to salt-stress conditions. Proteomics. 2010;10:445-57 pubmed publisher
    b>Corynebacterium glutamicum is one of the biotechnologically most important microorganisms because of its ability to enrich amino acids extracellularly. Hence, C...
  44. Klaffl S, Eikmanns B. Genetic and functional analysis of the soluble oxaloacetate decarboxylase from Corynebacterium glutamicum. J Bacteriol. 2010;192:2604-12 pubmed publisher
    ..Consistently, overexpression of the odx gene in an L-lysine-producing strain of C. glutamicum led to accumulation of less L-lysine. However, inactivation of the odx gene did not improve L-lysine production under the conditions tested. ..
  45. Becker J, Wittmann C. Bio-based production of chemicals, materials and fuels -Corynebacterium glutamicum as versatile cell factory. Curr Opin Biotechnol. 2012;23:631-40 pubmed publisher
    Since their discovery almost 60 years ago, Corynebacterium glutamicum and related subspecies are writing a remarkable success story in industrial biotechnology...
  46. Takeshita R, Ito H, Wachi M. A role of the cspA gene encoding a mycolyltransferase in the growth under alkaline conditions of Corynebacterium glutamicum. Biosci Biotechnol Biochem. 2010;74:1617-23 pubmed
    b>Corynebacterium glutamicum is widely used in the industrial production of amino acids. Producer strains are generated by classical random mutagenesis, and therefore have detrimental characteristics caused by unnecessary mutations...
  47. Zhao K, Huang Y, Chen X, Wang N, Liu S. PcaO positively regulates pcaHG of the beta-ketoadipate pathway in Corynebacterium glutamicum. J Bacteriol. 2010;192:1565-72 pubmed publisher
    ..which is involved in regulation of the protocatechuate (PCA) branch of the beta-ketoadipate pathway in Corynebacterium glutamicum. PcaO is an atypical large ATP-binding LuxR family (LAL)-type regulator and does not have a Walker A ..
  48. Noack S, Nöh K, Moch M, Oldiges M, Wiechert W. Stationary versus non-stationary (13)C-MFA: a comparison using a consistent dataset. J Biotechnol. 2011;154:179-90 pubmed publisher
    ..for directly comparing both methods a (13)C-labeling experiment in a fed-batch cultivation with a Corynebacterium glutamicum lysine producer was carried out...
  49. Seibold G, Breitinger K, Kempkes R, Both L, Kramer M, Dempf S, et al. The glgB-encoded glycogen branching enzyme is essential for glycogen accumulation in Corynebacterium glutamicum. Microbiology. 2011;157:3243-51 pubmed publisher
    b>Corynebacterium glutamicum transiently accumulates glycogen as carbon capacitor during the early exponential growth phase in media containing carbohydrates...
  50. Yim S, An S, Kang M, Lee J, Jeong K. Isolation of fully synthetic promoters for high-level gene expression in Corynebacterium glutamicum. Biotechnol Bioeng. 2013;110:2959-69 pubmed publisher
    b>Corynebacterium glutamicum is an important industrial organism that is widely used in the production of amino acids, nucleotides and vitamins. To extend its product spectrum and improve productivity, C...
  51. Park M, Cho S, Lee H, Sibley C, Rhie H. Alternative thymidylate synthase, ThyX, involved in Corynebacterium glutamicum ATCC 13032 survival during stationary growth phase. FEMS Microbiol Lett. 2010;307:128-34 pubmed publisher
    ..search has shown the presence of a gene homologous to an alternative thymidylate synthase (TS), thyX, in Corynebacterium glutamicum ATCC 13032...
  52. Dupres V, Alsteens D, Pauwels K, Dufrêne Y. In vivo imaging of S-layer nanoarrays on Corynebacterium glutamicum. Langmuir. 2009;25:9653-5 pubmed publisher
    ..Here we use atomic force microscopy (AFM) to visualize S-layer nanoarrays on living Corynebacterium glutamicum bacteria. We demonstrate the presence of two highly ordered surface layers...
  53. Buschke N, Schröder H, Wittmann C. Metabolic engineering of Corynebacterium glutamicum for production of 1,5-diaminopentane from hemicellulose. Biotechnol J. 2011;6:306-17 pubmed publisher
    ..For this purpose, the metabolism of 1,5-diaminopentane-producing Corynebacterium glutamicum was engineered to the use of the C(5) sugar xylose...
  54. Bartek T, Blombach B, Zönnchen E, Makus P, Lang S, Eikmanns B, et al. Importance of NADPH supply for improved L-valine formation in Corynebacterium glutamicum. Biotechnol Prog. 2010;26:361-71 pubmed publisher
    ..L-Valine yield increased from 0.49 +/- 0.13 to 0.67 +/- 0.03 mol Val/mol Glc, and, concomitantly, the formation of by-products such as pyruvate was reduced. ..
  55. Garc a Nafr a J, Baumgart M, Bott M, Wilkinson A, Wilson K. The Corynebacterium glutamicum aconitase repressor: scratching around for crystals. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010;66:1074-7 pubmed publisher
    ..was the first transcription factor to be identified in the regulation of the tricarboxylic acid cycle in Corynebacterium glutamicum, an organism that is of special industrial interest and is an emerging model organism for ..
  56. Lindner S, Niederholtmeyer H, Schmitz K, Schoberth S, Wendisch V. Polyphosphate/ATP-dependent NAD kinase of Corynebacterium glutamicum: biochemical properties and impact of ppnK overexpression on lysine production. Appl Microbiol Biotechnol. 2010;87:583-93 pubmed publisher
    ..Here, the cg1601/ppnK gene product from Corynebacterium glutamicum genome was purified from recombinant Escherichia coli and enzymatic characterization revealed its ..
  57. Huber R, Palmen T, Ryk N, Hillmer A, Luft K, Kensy F, et al. Replication methods and tools in high-throughput cultivation processes - recognizing potential variations of growth and product formation by on-line monitoring. BMC Biotechnol. 2010;10:22 pubmed publisher
    ..Finally, a high-throughput cultivation process was simulated with Corynebacterium glutamicum pEKEx2-phoD-GFP, beginning at the colony picking step...
  58. Fukui K, Koseki C, Yamamoto Y, Nakamura J, Sasahara A, Yuji R, et al. Identification of succinate exporter in Corynebacterium glutamicum and its physiological roles under anaerobic conditions. J Biotechnol. 2011;154:25-34 pubmed publisher
    b>Corynebacterium glutamicum produces succinate from glucose via the reductive tricarboxylic acid cycle under microaerobic and anaerobic conditions. We identified a NCgl2130 gene of C...
  59. Yun J, Lee J, Yang K, Cho S, Kim A, Kwon Y, et al. Ethambutol-mediated cell wall modification in recombinant Corynebacterium glutamicum increases the biotransformation rates of cyclohexanone derivatives. Bioprocess Biosyst Eng. 2012;35:211-6 pubmed publisher
    The effects of structural modification of cell wall on the biotransformation capability by recombinant Corynebacterium glutamicum cells, expressing the chnB gene encoding cyclohexanone monooxygenase of Acinetobacter calcoaceticus NCIMB ..
  60. Trötschel C, Albaum S, Wolff D, Schröder S, Goesmann A, Nattkemper T, et al. Protein turnover quantification in a multilabeling approach: from data calculation to evaluation. Mol Cell Proteomics. 2012;11:512-26 pubmed publisher
    ..shock response, a well-understood regulatory mechanism in bacteria, on the biotechnologically relevant Corynebacterium glutamicum. Utilizing a multilabeling approach using both heavy stable nitrogen as well as carbon isotopes cells ..
  61. Toyoda K, Teramoto H, Inui M, Yukawa H. The ldhA gene, encoding fermentative L-lactate dehydrogenase of Corynebacterium glutamicum, is under the control of positive feedback regulation mediated by LldR. J Bacteriol. 2009;191:4251-8 pubmed publisher
    b>Corynebacterium glutamicum ldhA encodes L-lactate dehydrogenase, a key enzyme that couples L-lactate production to reoxidation of NADH formed during glycolysis...
  62. Jung S, Chun J, Yim S, Lee S, Cheon C, Song E, et al. Transcriptional regulation of histidine biosynthesis genes in Corynebacterium glutamicum. Can J Microbiol. 2010;56:178-87 pubmed publisher
    b>Corynebacterium glutamicum, a gram-positive bacterium, has been widely used for industrial amino acid production...
  63. Smith K, Cho K, Liao J. Engineering Corynebacterium glutamicum for isobutanol production. Appl Microbiol Biotechnol. 2010;87:1045-55 pubmed publisher
    ..2-keto acids are precursors of amino acids, we aimed to construct an isobutanol production platform in Corynebacterium glutamicum, a well-known amino-acid-producing microorganism...
  64. Plassmeier J, Persicke M, Pühler A, Sterthoff C, Ruckert C, Kalinowski J. Molecular characterization of PrpR, the transcriptional activator of propionate catabolism in Corynebacterium glutamicum. J Biotechnol. 2012;159:1-11 pubmed publisher
    The 2-methylcitrate cycle is used to metabolize propionate in Corynebacterium glutamicum. The regulator, PrpR (Cg0800), of the prpDBC2 operon was identified and characterized...
  65. Kramer R. Osmosensing and osmosignaling in Corynebacterium glutamicum. Amino Acids. 2009;37:487-97 pubmed publisher
    The Gram-positive soil bacterium Corynebacterium glutamicum is used in microbial biotechnology for the large-scale production of amino acids, e.g., L: -glutamate and L: -lysine...
  66. Frantom P, Coward J, Blanchard J. UDP-(5F)-GlcNAc acts as a slow-binding inhibitor of MshA, a retaining glycosyltransferase. J Am Chem Soc. 2010;132:6626-7 pubmed publisher
    ..acts as a slow-binding, competitive inhibitor of the retaining glycosyltransferase MshA from Corynebacterium glutamicum (K(i) approximately 1.6 muM)...
  67. Kind S, Jeong W, Schröder H, Zelder O, Wittmann C. Identification and elimination of the competing N-acetyldiaminopentane pathway for improved production of diaminopentane by Corynebacterium glutamicum. Appl Environ Microbiol. 2010;76:5175-80 pubmed publisher
    The present work describes the development of a superior strain of Corynebacterium glutamicum for diaminopentane (cadaverine) production aimed at the identification and deletion of the underlying unknown N-acetyldiaminopentane pathway...
  68. Zhao Z, Ding J, Li T, Zhou N, Liu S. The ncgl1108 (PheP (Cg)) gene encodes a new L-Phe transporter in Corynebacterium glutamicum. Appl Microbiol Biotechnol. 2011;90:2005-13 pubmed publisher
    b>Corynebacterium glutamicum played a central role in the establishment of fermentative production of amino acids, and it is a model for genetic and physiological studies...
  69. van Ooyen J, Noack S, Bott M, Reth A, Eggeling L. Improved L-lysine production with Corynebacterium glutamicum and systemic insight into citrate synthase flux and activity. Biotechnol Bioeng. 2012;109:2070-81 pubmed publisher
    We here developed a series of Corynebacterium glutamicum strains with gradual decreased specific citrate synthase (CS) activity and quantified in a multifaceted approach the consequences of residual activity on the transcriptome, ..
  70. Tsuchidate T, Tateno T, Okai N, Tanaka T, Ogino C, Kondo A. Glutamate production from ?-glucan using endoglucanase-secreting Corynebacterium glutamicum. Appl Microbiol Biotechnol. 2011;90:895-901 pubmed publisher
    We demonstrate glutamate production from ?-glucan using endoglucanase (EG)-expressing Corynebacterium glutamicum. The signal sequence torA derived from Escherichia coli K12, which belongs to the Tat pathway, was suitable for secreting EG ..
  71. Yamamoto S, Sakai M, Inui M, Yukawa H. Diversity of metabolic shift in response to oxygen deprivation in Corynebacterium glutamicum and its close relatives. Appl Microbiol Biotechnol. 2011;90:1051-61 pubmed publisher
    Oxygen-deprived Corynebacterium glutamicum R cells remain metabolically active, producing considerable amounts of organic acids even when not actively growing. We compared the proficiencies of C...
  72. Nesvera J, Pátek M. Tools for genetic manipulations in Corynebacterium glutamicum and their applications. Appl Microbiol Biotechnol. 2011;90:1641-54 pubmed publisher
    b>Corynebacterium glutamicum is an important industrial producer of various amino acids with great potential for the production of other metabolites. The complete genome sequences of two C...
  73. Song Y, Matsumoto K, Yamada M, Gohda A, Brigham C, Sinskey A, et al. Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production. Appl Microbiol Biotechnol. 2012;93:1917-25 pubmed publisher
    ..polymer production system to a practical stage by using metabolically engineered Gram-positive bacterium Corynebacterium glutamicum as an endotoxin-free platform. We designed metabolic pathways in C...
  74. Wieschalka S, Blombach B, Eikmanns B. Engineering Corynebacterium glutamicum for the production of pyruvate. Appl Microbiol Biotechnol. 2012;94:449-59 pubmed publisher
    A Corynebacterium glutamicum strain with inactivated pyruvate dehydrogenase complex and a deletion of the gene encoding the pyruvate:quinone oxidoreductase produces about 19 mM L: -valine, 28 mM L: -alanine and about 55 mM pyruvate ..
  75. Zhang Y, Shang X, Lai S, Zhang G, Liang Y, Wen T. Development and application of an arabinose-inducible expression system by facilitating inducer uptake in Corynebacterium glutamicum. Appl Environ Microbiol. 2012;78:5831-8 pubmed publisher
    b>Corynebacterium glutamicum is currently used for the industrial production of a variety of biological materials...
  76. Batt S, Jabeen T, Mishra A, Veerapen N, Krumbach K, Eggeling L, et al. Acceptor substrate discrimination in phosphatidyl-myo-inositol mannoside synthesis: structural and mutational analysis of mannosyltransferase Corynebacterium glutamicum PimB'. J Biol Chem. 2010;285:37741-52 pubmed publisher
    ..Herein, we report the crystal structure of mannosyltransferase Corynebacterium glutamicum PimB' in complex with nucleotide to a resolution of 2.0 Å...
  77. Zhao Z, Ding J, Ma W, Zhou N, Liu S. Identification and characterization of ?-aminobutyric acid uptake system GabPCg (NCgl0464) in Corynebacterium glutamicum. Appl Environ Microbiol. 2012;78:2596-601 pubmed publisher
    b>Corynebacterium glutamicum is widely used for industrial production of various amino acids and vitamins, and there is growing interest in engineering this bacterium for more commercial bioproducts such as ?-aminobutyric acid (GABA)...
  78. Holátko J, Silar R, Rabatinová A, Sanderová H, Halada P, Nesvera J, et al. Construction of in vitro transcription system for Corynebacterium glutamicum and its use in the recognition of promoters of different classes. Appl Microbiol Biotechnol. 2012;96:521-9 pubmed publisher
    To facilitate transcription studies in Corynebacterium glutamicum, we have developed an in vitro transcription system for this bacterium used as an industrial producer of amino acids and a model organism for actinobacteria...
  79. Birch H, Alderwick L, Rittmann D, Krumbach K, Etterich H, Grzegorzewicz A, et al. Identification of a terminal rhamnopyranosyltransferase (RptA) involved in Corynebacterium glutamicum cell wall biosynthesis. J Bacteriol. 2009;191:4879-87 pubmed publisher
    A bioinformatics approach identified a putative integral membrane protein, NCgl0543, in Corynebacterium glutamicum, with 13 predicted transmembrane domains and a glycosyltransferase motif (RXXDE), features that are common to the ..
  80. Yoshida A, Tomita T, Kuzuyama T, Nishiyama M. Mechanism of concerted inhibition of alpha2beta2-type hetero-oligomeric aspartate kinase from Corynebacterium glutamicum. J Biol Chem. 2010;285:27477-86 pubmed publisher
    ..AK from Corynebacterium glutamicum (CgAK), a bacterium used for industrial fermentation of amino acids, including glutamate and lysine, is ..
  81. Niimi S, Suzuki N, Inui M, Yukawa H. Metabolic engineering of 1,2-propanediol pathways in Corynebacterium glutamicum. Appl Microbiol Biotechnol. 2011;90:1721-9 pubmed publisher
    We analyzed 1,2-propanediol (1,2-PD) production in metabolically engineered Corynebacterium glutamicum. Wild-type C. glutamicum produced 93 ?M 1,2-PD after 132 h incubation under aerobic conditions...
  82. Kato O, Youn J, Stansen K, Matsui D, Oikawa T, Wendisch V. Quinone-dependent D-lactate dehydrogenase Dld (Cg1027) is essential for growth of Corynebacterium glutamicum on D-lactate. BMC Microbiol. 2010;10:321 pubmed publisher
    b>Corynebacterium glutamicum is able to grow with lactate as sole or combined carbon and energy source. Quinone-dependent L-lactate dehydrogenase LldD is known to be essential for utilization of L-lactate by C. glutamicum...
  83. Zhang Y, Shang X, Deng A, Chai X, Lai S, Zhang G, et al. Genetic and biochemical characterization of Corynebacterium glutamicum ATP phosphoribosyltransferase and its three mutants resistant to feedback inhibition by histidine. Biochimie. 2012;94:829-38 pubmed publisher
    ..Here, we report the genetic and biochemical characterization of such an enzyme, HisG(Cg), from Corynebacterium glutamicum, including site-directed mutagenesis of the histidine-binding site for the first time...
  84. Sawada K, Zen in S, Wada M, Yokota A. Metabolic changes in a pyruvate kinase gene deletion mutant of Corynebacterium glutamicum ATCC 13032. Metab Eng. 2010;12:401-7 pubmed publisher
    To investigate primary effects of a pyruvate kinase (PYK) defect on glucose metabolism in Corynebacterium glutamicum, a pyk-deleted mutant was derived from wild-type C...
  85. Fiuza M, Letek M, Leiba J, Villadangos A, Vaquera J, Zanella Cleon I, et al. Phosphorylation of a novel cytoskeletal protein (RsmP) regulates rod-shaped morphology in Corynebacterium glutamicum. J Biol Chem. 2010;285:29387-97 pubmed publisher
    ..this protein is an essential cytoskeletal element needed for maintenance of the rod-shaped morphology of Corynebacterium glutamicum, and it was therefore renamed RsmP (rod-shaped morphology protein)...
  86. Teramoto H, Inui M, Yukawa H. Transcriptional regulators of multiple genes involved in carbon metabolism in Corynebacterium glutamicum. J Biotechnol. 2011;154:114-25 pubmed publisher
    b>Corynebacterium glutamicum, a high-GC Gram-positive soil bacterium, has been used in development of bioprocesses for production of various compounds such as amino acids, organic acids, and alcohols...
  87. Teramoto H, Watanabe K, Suzuki N, Inui M, Yukawa H. High yield secretion of heterologous proteins in Corynebacterium glutamicum using its own Tat-type signal sequence. Appl Microbiol Biotechnol. 2011;91:677-87 pubmed publisher
    ..Tat-type secretory signal peptide of CgR0949 to demonstrate a high-yield protein secretion system of Corynebacterium glutamicum. The resultant secretion vector facilitated effective secretion of active-form GFP (20 mg l(-1)) into C...
  88. Li S, Wen J, Jia X. Engineering Bacillus subtilis for isobutanol production by heterologous Ehrlich pathway construction and the biosynthetic 2-ketoisovalerate precursor pathway overexpression. Appl Microbiol Biotechnol. 2011;91:577-89 pubmed publisher
    ..subtilis, ketol-acid reductoisomerase, and dihydroxy-acid dehydratase of Corynebacterium glutamicum responsible for 2-ketoisovalerate precursor biosynthesis showed that acetolactate synthase played an ..
  89. Schneider J, Wendisch V. Biotechnological production of polyamines by bacteria: recent achievements and future perspectives. Appl Microbiol Biotechnol. 2011;91:17-30 pubmed publisher
    ..Only recently, researchers have investigated the potential of amino acid-producing strains of Corynebacterium glutamicum and Escherichia coli for polyamine production...
  90. Youn J, Jolkver E, Kramer R, Marin K, Wendisch V. Characterization of the dicarboxylate transporter DctA in Corynebacterium glutamicum. J Bacteriol. 2009;191:5480-8 pubmed publisher
    ..A member of this family, dicarboxylate transporter A (DctA) from Corynebacterium glutamicum, was characterized to catalyze uptake of the C(4)-dicarboxylates succinate, fumarate, and l-malate, ..
  91. Gopinath V, Meiswinkel T, Wendisch V, Nampoothiri K. Amino acid production from rice straw and wheat bran hydrolysates by recombinant pentose-utilizing Corynebacterium glutamicum. Appl Microbiol Biotechnol. 2011;92:985-96 pubmed publisher
    b>Corynebacterium glutamicum wild type lacks the ability to utilize the pentose fractions of lignocellulosic hydrolysates, but it is known that recombinants expressing the araBAD operon and/or the xylA gene from Escherichia coli are able to ..
  92. Grünberger A, Paczia N, Probst C, Schendzielorz G, Eggeling L, Noack S, et al. A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level. Lab Chip. 2012;12:2060-8 pubmed publisher
    ..of industrially relevant bacteria with single cell resolution (in particular Escherichia coli and Corynebacterium glutamicum) starting from one single mother cell to densely packed cultures is demonstrated...