streptomyces lividans

Summary

Summary: An actinomycete used for production of commercial ANTIBIOTICS and as a host for gene cloning.

Top Publications

  1. You D, Wang L, Yao F, Zhou X, Deng Z. A novel DNA modification by sulfur: DndA is a NifS-like cysteine desulfurase capable of assembling DndC as an iron-sulfur cluster protein in Streptomyces lividans. Biochemistry. 2007;46:6126-33 pubmed
    A novel DNA modification system by sulfur (S) in Streptomyces lividans 66 was reported to be encoded by a cluster of five genes designated dndA-E [Zhou, X., He, X., Liang, J., Li, A., Xu, T., Kieser, T., Helmann, J. D., and Deng, Z...
  2. Giardina A, Alduina R, Gottardi E, Di Caro V, Sussmuth R, Puglia A. Two heterologously expressed Planobispora rosea proteins cooperatively induce Streptomyces lividans thiostrepton uptake and storage from the extracellular medium. Microb Cell Fact. 2010;9:44 pubmed publisher
    ..Two Planobispora rosea orfs are responsible for increasing intracellular amounts and storage of thiostrepton in Streptomyces lividans.
  3. He X, Ou H, Yu Q, Zhou X, Wu J, Liang J, et al. Analysis of a genomic island housing genes for DNA S-modification system in Streptomyces lividans 66 and its counterparts in other distantly related bacteria. Mol Microbiol. 2007;65:1034-48 pubmed
    The complete sequence (92 770 bp) of a genomic island (GI) named SLG from Streptomyces lividans 66, encoding a novel DNA S-modification system (dnd), was determined. Its overall G+C content was 67...
  4. Wagner N, Osswald C, Biener R, Schwartz D. Comparative analysis of transcriptional activities of heterologous promoters in the rare actinomycete Actinoplanes friuliensis. J Biotechnol. 2009;142:200-4 pubmed publisher
    ..These experiments indicated that promoter strength in A. friuliensis did not correlate to activity in Streptomyces lividans. The ermE* promoter regarded as one of the strongest promoter in Streptomyces has only low activity in A...
  5. Brana A, Rodriguez M, Pahari P, Rohr J, Garcia L, Blanco G. Activation and silencing of secondary metabolites in Streptomyces albus and Streptomyces lividans after transformation with cosmids containing the thienamycin gene cluster from Streptomyces cattleya. Arch Microbiol. 2014;196:345-55 pubmed publisher
    Activation and silencing of antibiotic production was achieved in Streptomyces albus J1074 and Streptomyces lividans TK21 after introduction of genes within the thienamycin cluster from S. cattleya...
  6. Jayapal K, Lian W, Glod F, Sherman D, Hu W. Comparative genomic hybridizations reveal absence of large Streptomyces coelicolor genomic islands in Streptomyces lividans. BMC Genomics. 2007;8:229 pubmed
    The genomes of Streptomyces coelicolor and Streptomyces lividans bear a considerable degree of synteny. While S. coelicolor is the model streptomycete for studying antibiotic synthesis and differentiation, S...
  7. Fedoryshyn M, Welle E, Bechthold A, Luzhetskyy A. Functional expression of the Cre recombinase in actinomycetes. Appl Microbiol Biotechnol. 2008;78:1065-70 pubmed publisher
    ..7% optimized for expression in high-GC bacteria and demonstrated this gene to be functional in Streptomyces lividans. Using the synthetic cre(a) gene, we have removed an apramycin resistance gene flanked by loxP sites from ..
  8. Liu G, Ou H, Wang T, Li L, Tan H, Zhou X, et al. Cleavage of phosphorothioated DNA and methylated DNA by the type IV restriction endonuclease ScoMcrA. PLoS Genet. 2010;6:e1001253 pubmed publisher
    ..We observed that simultaneous expression of the dndA-E gene cluster from Streptomyces lividans 66, which is responsible for the DNA S-modification, and the putative Streptomyces coelicolor A(3)2 Type ..
  9. Sun Y, He X, Liang J, Zhou X, Deng Z. Analysis of functions in plasmid pHZ1358 influencing its genetic and structural stability in Streptomyces lividans 1326. Appl Microbiol Biotechnol. 2009;82:303-10 pubmed publisher
    ..basis of its structural and segregational instability, compared to the high copy number plasmid pIJ101 of Streptomyces lividans 1326 from which it was derived...

More Information

Publications74

  1. Iwamoto M, Oiki S. Counting ion and water molecules in a streaming file through the open-filter structure of the K channel. J Neurosci. 2011;31:12180-8 pubmed publisher
    ..has been supported by crystallographic studies that demonstrated ion distribution in the structure of the Streptomyces lividans (KcsA) potassium channel...
  2. Sevillano L, Diaz M, Yamaguchi Y, Inouye M, Santamaría R. Identification of the first functional toxin-antitoxin system in Streptomyces. PLoS ONE. 2012;7:e32977 pubmed publisher
    ..a functional TA system in Streptomyces that is identical in two species routinely used in the laboratory: Streptomyces lividans and S. coelicolor...
  3. Eckstein F. Phosphorothioation of DNA in bacteria. Nat Chem Biol. 2007;3:689-90 pubmed
  4. Zhang Y, Wang W, Li Y. Cloning, expression, and purification of soluble human interleukin-4 receptor in Streptomyces. Protein Expr Purif. 2004;36:139-45 pubmed
    ..chain was cloned into a Streptomyces-Escherichia coli shuttle plasmid pSGLgpp and expressed secretly in Streptomyces lividans TK 24...
  5. Xu T, Liang J, Chen S, Wang L, He X, You D, et al. DNA phosphorothioation in Streptomyces lividans: mutational analysis of the dnd locus. BMC Microbiol. 2009;9:41 pubmed publisher
    ..A detailed mutational analysis of the individual genes within the dnd locus in Streptomyces lividans responsible for DNA phosphorothioation was performed and is described here...
  6. Xu D, Kwon H, Suh J. S-Adenosylmethionine induces BldH and activates secondary metabolism by involving the TTA-codon control of bldH expression in Streptomyces lividans. Arch Microbiol. 2008;189:419-26 pubmed
    ..SAM) to promote secondary metabolism was characterized in terms of bldH sl) expression in Streptomyces lividans. A previous study demonstrated that SAM, on application at 2 microM, induces the transcription of the strR ..
  7. Meschke H, Schrempf H. Streptomyces lividans inhibits the proliferation of the fungus Verticillium dahliae on seeds and roots of Arabidopsis thaliana. Microb Biotechnol. 2010;3:428-43 pubmed publisher
    ..As documented by video-microscopy, the soil bacterium Streptomyces lividans strongly reduces the germination of V. dahliae conidia, and the subsequent growth of hyphae...
  8. Miao V, Coëffet Legal M, Brian P, Brost R, Penn J, Whiting A, et al. Daptomycin biosynthesis in Streptomyces roseosporus: cloning and analysis of the gene cluster and revision of peptide stereochemistry. Microbiology. 2005;151:1507-23 pubmed
    ..A 128 kb region of S. roseosporus DNA was cloned and verified by heterologous expression in Streptomyces lividans to contain the daptomycin biosynthetic gene cluster (dpt)...
  9. Dwarakanath S, Chaplin A, Hough M, Rigali S, Vijgenboom E, Worrall J. Response to copper stress in Streptomyces lividans extends beyond genes under direct control of a copper-sensitive operon repressor protein (CsoR). J Biol Chem. 2012;287:17833-47 pubmed publisher
    A copper-sensitive operon repressor protein (CsoR) has been identified in Streptomyces lividans (CsoR(Sl)) and found to regulate copper homeostasis with attomolar affinity for Cu(I)...
  10. Cordero Morales J, Cuello L, Perozo E. Voltage-dependent gating at the KcsA selectivity filter. Nat Struct Mol Biol. 2006;13:319-22 pubmed
    ..A mechanism for voltage-dependent gating at the selectivity filter is proposed that is based on the reorientation of the carboxylic moiety of Glu71 and its influence in the conformational dynamics of the selectivity filter...
  11. Chen F, Zhang Z, Lin K, Qian T, Zhang Y, You D, et al. Crystal structure of the cysteine desulfurase DndA from Streptomyces lividans which is involved in DNA phosphorothioation. PLoS ONE. 2012;7:e36635 pubmed publisher
    ..In this study, we determined the crystal structure of Streptomyces lividans DndA in complex with its covalently bound cofactor PLP, to a resolution of 2.4 Å...
  12. Ueno M, Yamashita M, Hashimoto M, Hino M, Fujie A. Oxidative activities of heterologously expressed CYP107B1 and CYP105D1 in whole-cell biotransformation using Streptomyces lividans TK24. J Biosci Bioeng. 2005;100:567-72 pubmed
    ..of some xenobiotics, such as secondary metabolites or environmental pollutants, were expressed in Streptomyces lividans TK24 with the Streptomyces expression vector pIJ6021...
  13. D Huys P, Lule I, Vercammen D, Anne J, Van Impe J, Bernaerts K. Genome-scale metabolic flux analysis of Streptomyces lividans growing on a complex medium. J Biotechnol. 2012;161:1-13 pubmed publisher
    ..In this paper, a systematic and integrative approach is presented to determine metabolic fluxes in Streptomyces lividans TK24 grown on a nutritious and complex medium...
  14. González Cerón G, Miranda Olivares O, Servín Gonzalez L. Characterization of the methyl-specific restriction system of Streptomyces coelicolor A3(2) and of the role played by laterally acquired nucleases. FEMS Microbiol Lett. 2009;301:35-43 pubmed publisher
    ..Cloning of these genes in the close relative Streptomyces lividans increased the restriction of methylated DNA by this species, confirming their role as part of the methyl-..
  15. Bhate M, Wylie B, Tian L, McDermott A. Conformational dynamics in the selectivity filter of KcsA in response to potassium ion concentration. J Mol Biol. 2010;401:155-66 pubmed publisher
  16. Hegermann J, Lunsdorf H, Overbeck J, Schrempf H. Polyphosphate at the Streptomyces lividans cytoplasmic membrane is enhanced in the presence of the potassium channel KcsA. J Microsc. 2008;229:174-82 pubmed publisher
    The distribution of polyphosphate (polyP) within the cytoplasmic membrane of Streptomyces lividans hyphae or protoplasts has been determined at high spatial resolution by elemental mapping using energy-filtered electron microscopy (EFTEM)..
  17. Zhou X, He X, Liang J, Li A, Xu T, Kieser T, et al. A novel DNA modification by sulphur. Mol Microbiol. 2005;57:1428-38 pubmed
    b>Streptomyces lividans has a novel DNA modification, which sensitises its DNA to degradation during electrophoresis (the Dnd phenotype)...
  18. Zhang B, Wang B, Wang P, Cao Z, Huang E, Hao J, et al. Enzymatic characterization of a monomeric isocitrate dehydrogenase from Streptomyces lividans TK54. Biochimie. 2009;91:1405-10 pubmed publisher
    ..we report for the first time the enzymatic characterization of a monomeric NADP(+)-dependent IDH from Streptomyces lividans TK54 (SlIDH)...
  19. Manteca A, Claessen D, Lopez Iglesias C, Sanchez J. Aerial hyphae in surface cultures of Streptomyces lividans and Streptomyces coelicolor originate from viable segments surviving an early programmed cell death event. FEMS Microbiol Lett. 2007;274:118-25 pubmed
    ..cell death event takes place during early growth of the substrate mycelium in Streptomyces coelicolor and Streptomyces lividans. Subsequently, a second mycelium starts to develop from the remaining viable segments of these substrate ..
  20. De Keersmaeker S, Vrancken K, Van Mellaert L, Anne J, Geukens N. The Tat pathway in Streptomyces lividans: interaction of Tat subunits and their role in translocation. Microbiology. 2007;153:1087-94 pubmed
    ..The Tat system of Streptomyces lividans consists of TatA, TatB and TatC, unlike most Gram-positive bacteria, which only have TatA and TatC ..
  21. Vrancken K, De Keersmaeker S, Geukens N, Lammertyn E, Anne J, Van Mellaert L. pspA overexpression in Streptomyces lividans improves both Sec- and Tat-dependent protein secretion. Appl Microbiol Biotechnol. 2007;73:1150-7 pubmed
    ..evaluated the importance of the phage-shock protein A (PspA) homologue on the protein secretion yield in Streptomyces lividans. The PspA protein is supposed to play a role in the maintenance of the proton motive force (PMF)...
  22. Escutia M, Val G, Palacin A, Geukens N, Anne J, Mellado R. Compensatory effect of the minor Streptomyces lividans type I signal peptidases on the SipY major signal peptidase deficiency as determined by extracellular proteome analysis. Proteomics. 2006;6:4137-46 pubmed
    The developmentally complex bacterium Streptomyces lividans has the ability to produce and secrete a significant amount of protein and possesses four different type I signal peptidase genes (sipW, sipX, sipY and sipZ) that are unusually ..
  23. De Keersmaeker S, Vrancken K, Van Mellaert L, Lammertyn E, Anne J, Geukens N. Evaluation of TatABC overproduction on Tat- and Sec-dependent protein secretion in Streptomyces lividans. Arch Microbiol. 2006;186:507-12 pubmed
    ..twin-arginine translocation (Tat) route seems to play an important role for protein secretion in Streptomyces lividans in whose genome tatA, tatB and tatC have been identified...
  24. Bellier A, Gominet M, Mazodier P. Post-translational control of the Streptomyces lividans ClgR regulon by ClpP. Microbiology. 2006;152:1021-7 pubmed
    It has been shown previously that expression of the Streptomyces lividans clpP1P2 operon, encoding proteolytic subunits of the Clp complex, the clpC1 gene, encoding the ATPase subunit, and the lon gene, encoding another ATP-dependent ..
  25. Lussier F, Denis F, Shareck F. Adaptation of the highly productive T7 expression system to Streptomyces lividans. Appl Environ Microbiol. 2010;76:967-70 pubmed publisher
    b>Streptomyces lividans is a Gram-positive bacterium known for its remarkable secretion efficiency and low extracellular protease activity. In the present work, we adapted the highly productive T7 expression system to S. lividans...
  26. Uysal S, Vásquez V, Tereshko V, Esaki K, Fellouse F, Sidhu S, et al. Crystal structure of full-length KcsA in its closed conformation. Proc Natl Acad Sci U S A. 2009;106:6644-9 pubmed publisher
    ..Functional analysis of the full-length KcsA-Fab complex suggests that the C-terminal bundle remains whole during gating. We suggest that this structure likely represents the physiologically relevant closed conformation of KcsA...
  27. Zhou X, He X, Li A, Lei F, Kieser T, Deng Z. Streptomyces coelicolor A3(2) lacks a genomic island present in the chromosome of Streptomyces lividans 66. Appl Environ Microbiol. 2004;70:7110-8 pubmed
    b>Streptomyces lividans ZX1 has become a preferred host for DNA cloning in Streptomyces species over its progenitor, the wild-type strain 66 (stock number 1326 from the John Innes Center collection), especially when stable DNA is crucial ..
  28. Gauthier C, Li H, Morosoli R. Increase in xylanase production by Streptomyces lividans through simultaneous use of the Sec- and Tat-dependent protein export systems. Appl Environ Microbiol. 2005;71:3085-92 pubmed
    Xylanase B1 (XlnB1) from Streptomyces lividans is a protein consisting of two discrete structural and functional units, an N-terminal catalytic domain and a C-terminal substrate binding domain...
  29. Noda S, Ito Y, Shimizu N, Tanaka T, Ogino C, Kondo A. Over-production of various secretory-form proteins in Streptomyces lividans. Protein Expr Purif. 2010;73:198-202 pubmed publisher
    b>Streptomyces lividans is known to produce large amounts of proteins in culture supernatants...
  30. Ayala J, Pimienta E, Rodriguez C, Anne J, Vallin C, Milanés M, et al. Use of Strep-tag II for rapid detection and purification of Mycobacterium tuberculosis recombinant antigens secreted by Streptomyces lividans. J Microbiol Methods. 2013;94:192-8 pubmed publisher
    ..tuberculosis immunodominant antigens in Streptomyces lividans and their subsequent downstream processing...
  31. Xu M, Zhu Y, Zhang R, Shen M, Jiang W, Zhao G, et al. Characterization of the genetic components of Streptomyces lividans linear plasmid SLP2 for replication in circular and linear modes. J Bacteriol. 2006;188:6851-7 pubmed
    The nucleotide sequence of Streptomyces lividans linear plasmid SLP2 consists of 50,410 bp (C. H. Huang, C. Y. Chen, H. H. Tsai, C. Chen, Y. S. Lin, and C. W. Chen, Mol. Microbiol. 47:1563-1576, 2003)...
  32. Cuello L, Jogini V, Cortes D, Perozo E. Structural mechanism of C-type inactivation in K(+) channels. Nature. 2010;466:203-8 pubmed publisher
    ..Here we report the crystal structure of the Streptomyces lividans K(+) channel KcsA in its open-inactivated conformation and investigate the mechanism of C-type ..
  33. Negoda A, Negoda E, Xian M, Reusch R. Role of polyphosphate in regulation of the Streptomyces lividans KcsA channel. Biochim Biophys Acta. 2009;1788:608-14 pubmed publisher
    We examine the hypotheses that the Streptomyces lividans potassium channel KcsA is gated at neutral pH by the electrochemical potential, and that its selectivity and conductance are governed at the cytoplasmic face by interactions ..
  34. Lewis R, Laing E, Allenby N, Bucca G, Brenner V, Harrison M, et al. Metabolic and evolutionary insights into the closely-related species Streptomyces coelicolor and Streptomyces lividans deduced from high-resolution comparative genomic hybridization. BMC Genomics. 2010;11:682 pubmed publisher
    ..Here we use new experimentally optimised 104,000 × 60-mer probe arrays to characterize in detail the genomic differences between wild-type S. lividans 66, a derivative industrial strain, TK24, and S. coelicolor M145...
  35. Nepal K, Oh T, Sohng J. Heterologous production of paromamine in Streptomyces lividans TK24 using kanamycin biosynthetic genes from Streptomyces kanamyceticus ATCC12853. Mol Cells. 2009;27:601-8 pubmed publisher
    ..These plasmids were heterologously expressed into Streptomyces lividans TK24 independently and generated two recombinant strains named S. lividans Sk-2/SL and S...
  36. Wang L, Chen S, Xu T, Taghizadeh K, Wishnok J, Zhou X, et al. Phosphorothioation of DNA in bacteria by dnd genes. Nat Chem Biol. 2007;3:709-10 pubmed
    ..To our knowledge, unlike any other DNA or RNA modification systems, DNA phosphorothioation by dnd gene clusters is the first physiological modification described on the DNA backbone...
  37. Blundell K, Wilson M, Svistunenko D, Vijgenboom E, Worrall J. Morphological development and cytochrome c oxidase activity in Streptomyces lividans are dependent on the action of a copper bound Sco protein. Open Biol. 2013;3:120163 pubmed publisher
    ..We report on the Sco-like protein from Streptomyces lividans (Sco(Sl)) and present a series of experiments that firmly establish a role for Sco(Sl) as a copper ..
  38. Cuello L, Jogini V, Cortes D, Pan A, Gagnon D, Dalmas O, et al. Structural basis for the coupling between activation and inactivation gates in K(+) channels. Nature. 2010;466:272-5 pubmed publisher
    ..We propose that side-chain rearrangements at position 103 mechanically couple activation and inactivation in KcsA and a variety of other K(+) channels...
  39. Guyet A, Gominet M, Benaroudj N, Mazodier P. Regulation of the clpP1clpP2 operon by the pleiotropic regulator AdpA in Streptomyces lividans. Arch Microbiol. 2013;195:831-41 pubmed publisher
    ..the ClpP1 and ClpP2 peptidase subunits) affects morphological and physiological differentiation of Streptomyces lividans. Another key factor controlling Streptomyces differentiation is the pleiotropic transcriptional regulator ..
  40. Schaerlaekens K, Lammertyn E, Geukens N, De Keersmaeker S, Anne J, Van Mellaert L. Comparison of the Sec and Tat secretion pathways for heterologous protein production by Streptomyces lividans. J Biotechnol. 2004;112:279-88 pubmed
    ..In this study, we assessed the possibility of the Streptomyces lividans twin-arginine translocation (Tat) pathway to secrete the human proteins tumor necrosis factor (TNF) alpha ..
  41. Penn J, Li X, Whiting A, Latif M, Gibson T, Silva C, et al. Heterologous production of daptomycin in Streptomyces lividans. J Ind Microbiol Biotechnol. 2006;33:121-8 pubmed
    ..A21978C antibiotic complex are lipopeptides produced by Streptomyces roseosporus and also in recombinant Streptomyces lividans TK23 and TK64 strains, when a 128 kbp region of cloned S...
  42. Xu D, Kim T, Park Z, Lee S, Yang S, Kwon H, et al. A DNA-binding factor, ArfA, interacts with the bldH promoter and affects undecylprodigiosin production in Streptomyces lividans. Biochem Biophys Res Commun. 2009;379:319-23 pubmed publisher
    ..is conserved among many bacteria was identified using DNA affinity purification from the cell extracts of Streptomyces lividans. The interactions of ArfA with the adpA promoter from S. griseus and with the bldH promoter from S...
  43. Qi X, Jiang R, Yao C, Zhang R, Li Y. Expression, purification, and characterization of C-terminal amidated glucagon in Streptomyces lividans. J Microbiol Biotechnol. 2008;18:1076-80 pubmed
    ..were expressed under control of the strong constitutive promoter of aph and secretion signal melC1 in Streptomyces lividans. With Phenyl-Sepharose 6 FF, Q-Sepharose FF, SP-Sepharose FF chromatographies and HPLC, the peptide was ..
  44. Ricoux R, Dubuc R, Dupont C, Marechal J, Martin A, Sellier M, et al. Hemozymes peroxidase activity of artificial hemoproteins constructed from the Streptomyces lividans xylanase A and iron(III)-carboxy-substituted porphyrins. Bioconjug Chem. 2008;19:899-910 pubmed publisher
    ..5 was participating in the catalysis. Finally, a very interesting protective effect against oxidative degradation of the porphyrin was provided by the protein...
  45. Ghorbel S, Smirnov A, Chouayekh H, Sperandio B, Esnault C, Kormanec J, et al. Regulation of ppk expression and in vivo function of Ppk in Streptomyces lividans TK24. J Bacteriol. 2006;188:6269-76 pubmed
    The ppk gene of Streptomyces lividans encodes an enzyme catalyzing, in vitro, the reversible polymerization of the gamma phosphate of ATP into polyphosphate and was previously shown to play a negative role in the control of antibiotic ..
  46. Diaz M, Ferreras E, Moreno R, Yepes A, Berenguer J, Santamaría R. High-level overproduction of Thermus enzymes in Streptomyces lividans. Appl Microbiol Biotechnol. 2008;79:1001-8 pubmed publisher
    ..In this paper, we show that Streptomyces lividans is a suitable host for the expression of Thermus thermophilus genes and report the overproduction of the ..
  47. Vrancken K, Van Mellaert L, Anne J. Cloning and expression vectors for a Gram-positive host, Streptomyces lividans. Methods Mol Biol. 2010;668:97-107 pubmed publisher
    ..Org Lett 2:2401-2404, 2000). Furthermore, due to its high innate secretion capacity, it can be a superior system than E. coli for the production of many extra-cellular proteins...
  48. Kim Y, Sa S, Chang Y, Hong S, Hong Y. Overexpression of Shinorhizobium meliloti hemoprotein in Streptomyces lividans to enhance secondary metabolite production. J Microbiol Biotechnol. 2007;17:2066-70 pubmed
    ..than Vitreoscilla hemoglobin (Vhb) in promoting secondary metabolites production when overexpressed in Streptomyces lividans TK24. The transformant with sm (sm-transformant) produced 2...
  49. D az M, Esteban A, Fern ndez Abalos J, Santamar a R. The high-affinity phosphate-binding protein PstS is accumulated under high fructose concentrations and mutation of the corresponding gene affects differentiation in Streptomyces lividans. Microbiology. 2005;151:2583-92 pubmed publisher
    The secreted protein pattern of Streptomyces lividans depends on the carbon source present in the culture media...
  50. Cordero Morales J, Cuello L, Zhao Y, Jogini V, Cortes D, Roux B, et al. Molecular determinants of gating at the potassium-channel selectivity filter. Nat Struct Mol Biol. 2006;13:311-8 pubmed publisher
    ..These data establish a mechanistic basis for the role of the selectivity filter during channel activation and inactivation...
  51. Young T, Walsh C. Identification of the thiazolyl peptide GE37468 gene cluster from Streptomyces ATCC 55365 and heterologous expression in Streptomyces lividans. Proc Natl Acad Sci U S A. 2011;108:13053-8 pubmed publisher
    ..thiazolyl peptide GE37468 from Streptomyces ATCC 55365 and its heterologous expression in the model host Streptomyces lividans. GE37468 harbors an unusual ?-methyl-?-hydroxy-proline residue that may increase conformational rigidity ..
  52. Singh D, Kwon H, Rajkarnikar A, Suh J. Glucoamylase gene, vldI, is linked to validamycin biosynthesis in Streptomyces hygroscopicus var. limoneus, and vldADEFG confers validamycin production in Streptomyces lividans, revealing the role of VldE in glucose attachment. Gene. 2007;395:151-9 pubmed
    ..After confirming that genes vldABCDEFGH confer validamycin production ability to Streptomyces lividans, genes vldBCHI were eliminated from the expression construct and the remaining genes, vldADEFG, were ..
  53. Ghorbel S, Kormanec J, Artus A, Virolle M. Transcriptional studies and regulatory interactions between the phoR-phoP operon and the phoU, mtpA, and ppk genes of Streptomyces lividans TK24. J Bacteriol. 2006;188:677-86 pubmed
    The PhoR/PhoP two-component system of Streptomyces lividans was previously shown to allow the growth of the bacteria at low Pi concentrations and to negatively control antibiotic production...
  54. McMahon M, Guan C, Handelsman J, Thomas M. Metagenomic analysis of Streptomyces lividans reveals host-dependent functional expression. Appl Environ Microbiol. 2012;78:3622-9 pubmed publisher
    ..expressed genes, we developed tools for construction and functional screening of metagenomic libraries in Streptomyces lividans. We expanded on previously published protocols by constructing a system that enables retrieval and ..
  55. Cruz Morales P, Vijgenboom E, Iruegas Bocardo F, Girard G, Yañez Guerra L, Ramos Aboites H, et al. The genome sequence of Streptomyces lividans 66 reveals a novel tRNA-dependent peptide biosynthetic system within a metal-related genomic island. Genome Biol Evol. 2013;5:1165-75 pubmed publisher
    The complete genome sequence of the original isolate of the model actinomycete Streptomyces lividans 66, also referred to as 1326, was deciphered after a combination of next-generation sequencing platforms and a hybrid assembly pipeline...
  56. Hong B, Wang L, Lammertyn E, Geukens N, Van Mellaert L, Li Y, et al. Inactivation of the 20S proteasome in Streptomyces lividans and its influence on the production of heterologous proteins. Microbiology. 2005;151:3137-45 pubmed
    ..In order to evaluate the influence of the 20S proteasome on the production of heterologous proteins by Streptomyces lividans TK24, the production of a number of heterologous proteins, including soluble human tumour necrosis factor ..
  57. Vrancken K, Van Mellaert L, Anne J. Characterization of the Streptomyces lividans PspA response. J Bacteriol. 2008;190:3475-81 pubmed publisher
    ..This conservation indicates that PspA alone might play an important role in these bacteria. In Streptomyces lividans, a soil-borne gram-positive bacterium, the phage shock protein system consists only of the pspA gene...
  58. Sianidis G, Pozidis C, Becker F, Vrancken K, Sjoeholm C, Karamanou S, et al. Functional large-scale production of a novel Jonesia sp. xyloglucanase by heterologous secretion from Streptomyces lividans. J Biotechnol. 2006;121:498-507 pubmed
    ..subtilis or E. coli failed. In contrast, Xeg was successfully over-expressed and secreted in Streptomyces lividans TK24...
  59. Anne J, Maldonado B, Van Impe J, Van Mellaert L, Bernaerts K. Recombinant protein production and streptomycetes. J Biotechnol. 2012;158:159-67 pubmed publisher
    ..In this overview, we focus on Streptomyces lividans, a Gram-positive bacterium with a proven excellence in secretion capacity, as host for heterologous ..
  60. De Keersmaeker S, Van Mellaert L, Schaerlaekens K, Van Dessel W, Vrancken K, Lammertyn E, et al. Structural organization of the twin-arginine translocation system in Streptomyces lividans. FEBS Lett. 2005;579:797-802 pubmed
    ..Recently, genes encoding TatA, TatB and TatC homologues were identified in Streptomyces lividans and the functionality of the Tat pathway was demonstrated...
  61. Lee L, Chen Y, Kirby R, Chen C, Chen C. A multidrug efflux system is involved in colony growth in Streptomyces lividans. Microbiology. 2007;153:924-34 pubmed
    ..pair consisting of a TetR-family regulator (ebrS) and a major facilitator-family MDR pump (ebrC) gene in Streptomyces lividans, which is widely conserved in Streptomyces species...
  62. Ahmetagic A, Pemberton J. Stable high level expression of the violacein indolocarbazole anti-tumour gene cluster and the Streptomyces lividans amyA gene in E. coli K12. Plasmid. 2010;63:79-85 pubmed publisher
    ..In addition, E. coli K12 JM109 strongly expressed an alpha amylase gene (amyA) from Streptomyces lividans indicating that the S. lividans amyA promoter is highly active in E. coli K12 JM109...
  63. Dubeau M, Poulin Laprade D, Ghinet M, Brzezinski R. Properties of CsnR, the transcriptional repressor of the chitosanase gene, csnA, of Streptomyces lividans. J Bacteriol. 2011;193:2441-50 pubmed publisher
    ..sequence is present in the intergenic region preceding the chitosanase gene csnA (SSPG_06922) of Streptomyces lividans TK24...
  64. Nowruzi K, Elkamel A, Scharer J, Cossar D, Moo Young M. Development of a minimal defined medium for recombinant human interleukin-3 production by Streptomyces lividans 66. Biotechnol Bioeng. 2008;99:214-22 pubmed
    ..amino acids in defined minimal medium for the production of recombinant human interleukin 3 (rHuIL-3) by Streptomyces lividans. Starvation trials were carried out initially to narrow down the number of probable essential amino acids ..
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    ..This structural basis of the activation-coupled inactivation is closely related to the C-type inactivation of other K channels...