streptomyces coelicolor

Summary

Summary: A soil-dwelling actinomycete with a complex lifecycle involving mycelial growth and spore formation. It is involved in the production of a number of medically important ANTIBIOTICS.

Top Publications

  1. Waldvogel E, Herbig A, Battke F, Amin R, Nentwich M, Nieselt K, et al. The PII protein GlnK is a pleiotropic regulator for morphological differentiation and secondary metabolism in Streptomyces coelicolor. Appl Microbiol Biotechnol. 2011;92:1219-36 pubmed publisher
    GlnK is an important nitrogen sensor protein in Streptomyces coelicolor. Deletion of glnK results in a medium-dependent failure of aerial mycelium and spore formation and loss of antibiotic production...
  2. Wang G, Inaoka T, Okamoto S, Ochi K. A novel insertion mutation in Streptomyces coelicolor ribosomal S12 protein results in paromomycin resistance and antibiotic overproduction. Antimicrob Agents Chemother. 2009;53:1019-26 pubmed publisher
    ..resistance-associated mutation in rpsL, caused by the insertion of a glycine residue at position 92, in Streptomyces coelicolor ribosomal protein S12...
  3. An Y, Ahn B, Han A, Kim H, Chung K, Shin J, et al. Structural basis for the specialization of Nur, a nickel-specific Fur homolog, in metal sensing and DNA recognition. Nucleic Acids Res. 2009;37:3442-51 pubmed publisher
    ..a nickel-responsive transcription factor that controls nickel homeostasis and anti-oxidative response in Streptomyces coelicolor. Here we report the 2.4-A resolution crystal structure of Nur...
  4. Sandoval Calderón M, Geiger O, Guan Z, Barona Gomez F, Sohlenkamp C. A eukaryote-like cardiolipin synthase is present in Streptomyces coelicolor and in most actinobacteria. J Biol Chem. 2009;284:17383-90 pubmed publisher
    ..Searching the genome of the actinomycete Streptomyces coelicolor A3(2) we identified a gene coding for a putative Cls of the CDP-alcohol phosphatidyltransferase ..
  5. McKenzie N, Thaker M, Koteva K, Hughes D, Wright G, Nodwell J. Induction of antimicrobial activities in heterologous streptomycetes using alleles of the Streptomyces coelicolor gene absA1. J Antibiot (Tokyo). 2010;63:177-82 pubmed publisher
    ..approach to this problem, we have used alleles of a pleiotropic regulator of secondary metabolism from Streptomyces coelicolor to activate secondary biosynthetic gene clusters in heterologous streptomycetes...
  6. Vockenhuber M, Suess B. Streptomyces coelicolor sRNA scr5239 inhibits agarase expression by direct base pairing to the dagA coding region. Microbiology. 2012;158:424-35 pubmed publisher
    Transcriptional regulation of primary and secondary metabolism is well-studied in Streptomyces coelicolor, a model organism for antibiotic production and cell differentiation...
  7. den Hengst C, Tran N, Bibb M, Chandra G, Leskiw B, Buttner M. Genes essential for morphological development and antibiotic production in Streptomyces coelicolor are targets of BldD during vegetative growth. Mol Microbiol. 2010;78:361-79 pubmed
    BldD is a transcriptional regulator essential for morphological development and antibiotic production in Streptomyces coelicolor. Here we identify the BldD regulon by means of chromatin immunoprecipitation-microarray analysis (ChIP-chip)...
  8. Paradzik T, Ivic N, Filic Z, Manjasetty B, Herron P, Luic M, et al. Structure-function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor: implication of SsbB in chromosome segregation during sporulation. Nucleic Acids Res. 2013;41:3659-72 pubmed publisher
    The linear chromosome of Streptomyces coelicolor contains two paralogous ssb genes, ssbA and ssbB...
  9. Dangel V, Westrich L, Smith M, Heide L, Gust B. Use of an inducible promoter for antibiotic production in a heterologous host. Appl Microbiol Biotechnol. 2010;87:261-9 pubmed publisher
    ..The heterologous producer Streptomyces coelicolor M512 harboring the modified gene cluster produced, upon addition of 2 mg L(-1) of the inducer compound ..

More Information

Publications110 found, 100 shown here

  1. Pérez Redondo R, Rodríguez García A, Botas A, Santamarta I, Martin J, Liras P. ArgR of Streptomyces coelicolor is a versatile regulator. PLoS ONE. 2012;7:e32697 pubmed publisher
    ..Transcriptomic comparison by microarrays has been made between Streptomyces coelicolor M145 and its mutant S...
  2. Arthur C, Williams C, Pottage K, Płoskoń E, Findlow S, Burston S, et al. Structure and malonyl CoA-ACP transacylase binding of streptomyces coelicolor fatty acid synthase acyl carrier protein. ACS Chem Biol. 2009;4:625-36 pubmed publisher
    ..Mutagenesis of homologous helix II residues on the polyketide synthase ACP suggests that the PKS ACP may bind to MCAT in a different manner than the FAS counterpart. ..
  3. Duong A, Capstick D, Di Berardo C, Findlay K, Hesketh A, Hong H, et al. Aerial development in Streptomyces coelicolor requires sortase activity. Mol Microbiol. 2012;83:992-1005 pubmed publisher
    b>Streptomyces coelicolor is a multicellular bacterium whose life cycle encompasses three differentiated states: vegetative hyphae, aerial hyphae and spores...
  4. Rozas D, Gullón S, Mellado R. A novel two-component system involved in the transition to secondary metabolism in Streptomyces coelicolor. PLoS ONE. 2012;7:e31760 pubmed publisher
    ..METHODOLOGY/PRINCIPALFINDINGS: The Streptomyces coelicolor two genes operon SCO5784-SCO5785 encodes a two-component system, where SCO5784 encodes a histidine-kinase ..
  5. Salerno P, Larsson J, Bucca G, Laing E, Smith C, Flärdh K. One of the two genes encoding nucleoid-associated HU proteins in Streptomyces coelicolor is developmentally regulated and specifically involved in spore maturation. J Bacteriol. 2009;191:6489-500 pubmed publisher
    ..We show here that the two HU genes in Streptomyces coelicolor are differentially regulated and that hupS is specifically expressed during sporulation, while hupA is ..
  6. Manteca A, Sanchez J, Jung H, Schwämmle V, Jensen O. Quantitative proteomics analysis of Streptomyces coelicolor development demonstrates that onset of secondary metabolism coincides with hypha differentiation. Mol Cell Proteomics. 2010;9:1423-36 pubmed publisher
    ..study, we made a detailed proteomics analysis of the distinct developmental stages of solid confluent Streptomyces coelicolor cultures using iTRAQ (isobaric tags for relative and absolute quantitation) labeling and LC-MS/MS...
  7. Fernández Martínez L, Del Sol R, Evans M, Fielding S, Herron P, Chandra G, et al. A transposon insertion single-gene knockout library and new ordered cosmid library for the model organism Streptomyces coelicolor A3(2). Antonie Van Leeuwenhoek. 2011;99:515-22 pubmed publisher
    ..system employing in vitro shuttle transposon mutagenesis has been used to systematically mutagenise the Streptomyces coelicolor genome. To achieve the highest coverage, a new ordered cosmid library was also constructed...
  8. Rodríguez García A, Sola Landa A, Apel K, Santos Beneit F, Martin J. Phosphate control over nitrogen metabolism in Streptomyces coelicolor: direct and indirect negative control of glnR, glnA, glnII and amtB expression by the response regulator PhoP. Nucleic Acids Res. 2009;37:3230-42 pubmed publisher
    ..This work shows a phosphate control over the nitrogen metabolism in the model actinomycete Streptomyces coelicolor. Phosphate control of metabolism in Streptomyces is exerted by the two component system PhoR-PhoP...
  9. de Jong W, Wosten H, Dijkhuizen L, Claessen D. Attachment of Streptomyces coelicolor is mediated by amyloidal fimbriae that are anchored to the cell surface via cellulose. Mol Microbiol. 2009;73:1128-40 pubmed publisher
    The chaplin proteins ChpA-H enable the filamentous bacterium Streptomyces coelicolor to form reproductive aerial structures by assembling into surface-active amyloid-like fibrils...
  10. Stefanić Z, Vujaklija D, Luic M. Structure of the single-stranded DNA-binding protein from Streptomyces coelicolor. Acta Crystallogr D Biol Crystallogr. 2009;65:974-9 pubmed publisher
    The crystal structure of the single-stranded DNA-binding protein (SSB) from Streptomyces coelicolor, a filamentous soil bacterium with a complex life cycle and a linear chromosome, has been solved and refined at 2.1 A resolution...
  11. Arabolaza A, D Angelo M, Comba S, Gramajo H. FasR, a novel class of transcriptional regulator, governs the activation of fatty acid biosynthesis genes in Streptomyces coelicolor. Mol Microbiol. 2010;78:47-63 pubmed publisher
    ..In Streptomyces coelicolor we found that control of lipid homeostasis is accomplished, at least in part, through the transcriptional ..
  12. Gatewood M, Bralley P, Jones G. RNase III-dependent expression of the rpsO-pnp operon of Streptomyces coelicolor. J Bacteriol. 2011;193:4371-9 pubmed publisher
    We have examined the expression of the rpsO-pnp operon in an RNase III (rnc) mutant of Streptomyces coelicolor. Western blotting demonstrated that polynucleotide phosphorylase (PNPase) levels increased in the rnc mutant, JSE1880, ..
  13. Dela Cruz R, Gao Y, Penumetcha S, Sheplock R, Weng K, Chander M. Expression of the Streptomyces coelicolor SoxR regulon is intimately linked with actinorhodin production. J Bacteriol. 2010;192:6428-38 pubmed publisher
    ..the regulation and function of the SoxR regulon in the model antibiotic-producing filamentous bacterium Streptomyces coelicolor. Unlike the E. coli soxR deletion mutant, the S...
  14. Brault G, Shareck F, Hurtubise Y, L pine F, Doucet N. Isolation and characterization of EstC, a new cold-active esterase from Streptomyces coelicolor A3(2). PLoS ONE. 2012;7:e32041 pubmed publisher
    The genome sequence of Streptomyces coelicolor A3(2) contains more than 50 genes coding for putative lipolytic enzymes...
  15. Davis J, Brown B, Page R, Sello J. Study of PcaV from Streptomyces coelicolor yields new insights into ligand-responsive MarR family transcription factors. Nucleic Acids Res. 2013;41:3888-900 pubmed publisher
    ..Here, we describe how PcaV, a MarR family regulator in Streptomyces coelicolor, controls transcription of genes encoding ?-ketoadipate pathway enzymes through its interaction with the ..
  16. Lee H, Kim J, Kim P, Lee H, Kim E. Repression of antibiotic downregulator WblA by AdpA in Streptomyces coelicolor. Appl Environ Microbiol. 2013;79:4159-63 pubmed publisher
    The upstream region of antibiotic downregulatory wblA in Streptomyces coelicolor was found to contain AdpA binding motifs...
  17. McCormick J, Flärdh K. Signals and regulators that govern Streptomyces development. FEMS Microbiol Rev. 2012;36:206-31 pubmed publisher
    b>Streptomyces coelicolor is the genetically best characterized species of a populous genus belonging to the gram-positive Actinobacteria...
  18. Willemse J, van Wezel G. Imaging of Streptomyces coelicolor A3(2) with reduced autofluorescence reveals a novel stage of FtsZ localization. PLoS ONE. 2009;4:e4242 pubmed publisher
    ..Here we describe the development of a novel derivative of Streptomyces coelicolor A3(2) with strongly reduced autofluorescence, allowing the imaging of fluorescently labelled proteins at ..
  19. McKenzie N, Nodwell J. Transmembrane topology of the AbsA1 sensor kinase of Streptomyces coelicolor. Microbiology. 2009;155:1812-8 pubmed publisher
    ..The phosphorylated response regulator represses antibiotic biosynthesis operons in Streptomyces coelicolor. AbsA1 was predicted to have an atypical transmembrane topology, and the location of its signal-sensing ..
  20. Kallifidas D, Pascoe B, Owen G, Strain Damerell C, Hong H, Paget M. The zinc-responsive regulator Zur controls expression of the coelibactin gene cluster in Streptomyces coelicolor. J Bacteriol. 2010;192:608-11 pubmed publisher
    b>Streptomyces coelicolor mutants lacking the zinc-responsive Zur repressor are conditionally defective in sporulation, presumably due to the overexpression of one or more Zur target genes...
  21. Perez J, Munoz Dorado J, Brana A, Shimkets L, Sevillano L, Santamaría R. Myxococcus xanthus induces actinorhodin overproduction and aerial mycelium formation by Streptomyces coelicolor. Microb Biotechnol. 2011;4:175-83 pubmed publisher
    Interaction of the predatory myxobacterium Myxococcus xanthus with the non-motile, antibiotic producer Streptomyces coelicolor was examined using a variety of experimental approaches. Myxococcus xanthus cells prey on S...
  22. Gupta A, Nederlof I, Sottini S, Tepper A, Groenen E, Thomassen E, et al. Involvement of Tyr108 in the enzyme mechanism of the small laccase from Streptomyces coelicolor. J Am Chem Soc. 2012;134:18213-6 pubmed publisher
    The enzyme mechanism of the multicopper oxidase (MCO) SLAC from Streptomyces coelicolor was investigated by structural (XRD), spectroscopic (optical, EPR), and kinetics (stopped-flow) experiments on variants in which residue Tyr108 had ..
  23. Fowler Goldsworthy K, Gust B, Mouz S, Chandra G, Findlay K, Chater K. The actinobacteria-specific gene wblA controls major developmental transitions in Streptomyces coelicolor A3(2). Microbiology. 2011;157:1312-28 pubmed publisher
    The Streptomyces coelicolor A3(2) sporulation gene whiB is the paradigm of a family of genes (wbl, whiB-like) that are confined to actinobacteria. The chromosome of S...
  24. Castro Melchor M, Charaniya S, Karypis G, Takano E, Hu W. Genome-wide inference of regulatory networks in Streptomyces coelicolor. BMC Genomics. 2010;11:578 pubmed publisher
    ..of genomic tools and access to a large warehouse of transcriptome data for the model organism, Streptomyces coelicolor, provides incentive to decipher the intricacies of the regulatory cascades and develop biologically ..
  25. Gomez Escribano J, Bibb M. Engineering Streptomyces coelicolor for heterologous expression of secondary metabolite gene clusters. Microb Biotechnol. 2011;4:207-15 pubmed publisher
    We have constructed derivatives of Streptomyces coelicolor M145 as hosts for the heterologous expression of secondary metabolite gene clusters...
  26. Wasserstrom S, Grantcharova N, Ubhayasekera W, Ausmees N, Sandblad L, Fl rdh K. Non-sporulating ftsZ mutants in Streptomyces coelicolor reveal amino acid residues critical for FtsZ polymerization dynamics. Microbiology. 2013;159:890-901 pubmed publisher
    During sporulation of Streptomyces coelicolor, the cytokinetic protein FtsZ is assembled into dozens of regularly spaced Z rings, which orchestrate the division of aerial hyphae into spores...
  27. Gottelt M, Kol S, Gomez Escribano J, Bibb M, Takano E. Deletion of a regulatory gene within the cpk gene cluster reveals novel antibacterial activity in Streptomyces coelicolor A3(2). Microbiology. 2010;156:2343-53 pubmed publisher
    Genome sequencing of Streptomyces coelicolor A3(2) revealed an uncharacterized type I polyketide synthase gene cluster (cpk)...
  28. Hesketh A, Kock H, Mootien S, Bibb M. The role of absC, a novel regulatory gene for secondary metabolism, in zinc-dependent antibiotic production in Streptomyces coelicolor A3(2). Mol Microbiol. 2009;74:1427-44 pubmed publisher
    The availability of zinc was shown to have a marked influence on the biosynthesis of actinorhodin in Streptomyces coelicolor A3(2)...
  29. Hindra -, Pak P, Elliot M. Regulation of a novel gene cluster involved in secondary metabolite production in Streptomyces coelicolor. J Bacteriol. 2010;192:4973-82 pubmed publisher
    ..Here, we provide evidence for the contribution of a novel genetic locus to antibiotic production in Streptomyces coelicolor. The overexpression of a gene cluster comprising four protein-encoding genes (abeABCD) and an antisense ..
  30. Temuujin U, Chi W, Chang Y, Hong S. Identification and biochemical characterization of Sco3487 from Streptomyces coelicolor A3(2), an exo- and endo-type ?-agarase-producing neoagarobiose. J Bacteriol. 2012;194:142-9 pubmed publisher
    b>Streptomyces coelicolor can degrade agar, the main cell wall component of red macroalgae, for growth...
  31. Facey P, Hitchings M, Williams J, Skibinski D, Dyson P, Del Sol R. The evolution of an osmotically inducible dps in the genus Streptomyces. PLoS ONE. 2013;8:e60772 pubmed publisher
    ..Lastly, we identify a rare novel clade of Dps and show that a representative of these proteins in S. coelicolor possesses a dodecameric quaternary structure of high stability. ..
  32. D Alia D, Eggle D, Nieselt K, Hu W, Breitling R, Takano E. Deletion of the signalling molecule synthase ScbA has pleiotropic effects on secondary metabolite biosynthesis, morphological differentiation and primary metabolism in Streptomyces coelicolor A3(2). Microb Biotechnol. 2011;4:239-51 pubmed publisher
    ..In Streptomyces coelicolor, a group of signalling molecules called SCBs (S...
  33. Walshaw J, Gillespie M, Kelemen G. A novel coiled-coil repeat variant in a class of bacterial cytoskeletal proteins. J Struct Biol. 2010;170:202-15 pubmed publisher
    ..We recently demonstrated in Streptomyces coelicolor a cytoskeletal role of Scy, a large protein implicated in filamentous growth, whose sequence is dominated ..
  34. Okamoto S, Taguchi T, Ochi K, Ichinose K. Biosynthesis of actinorhodin and related antibiotics: discovery of alternative routes for quinone formation encoded in the act gene cluster. Chem Biol. 2009;16:226-36 pubmed publisher
    ..a two-component monooxygenase homologous to the ActVA-ORF5/ActVB system for actinorhodin biosynthesis in Streptomyces coelicolor A3(2). Here, we conducted molecular genetic and biochemical studies of this enzyme system...
  35. Wang L, Li C, Ni Y, Zhang J, Liu X, Xu J. Highly efficient synthesis of chiral alcohols with a novel NADH-dependent reductase from Streptomyces coelicolor. Bioresour Technol. 2011;102:7023-8 pubmed publisher
    An NADH-dependent reductase (ScCR) from Streptomyces coelicolor was discovered by genome mining for carbonyl reductases. ScCR was overexpressed in Escherichia coli BL21, purified to homogeneity and its catalytic properties were studied...
  36. Skalova T, Dohnalek J, Østergaard L, Østergaard P, Kolenko P, Duskova J, et al. The structure of the small laccase from Streptomyces coelicolor reveals a link between laccases and nitrite reductases. J Mol Biol. 2009;385:1165-78 pubmed publisher
    The X-ray structure of the two-domain laccase (small laccase) from Streptomyces coelicolor A3(2) was solved at 2.7-A resolution. The enzyme differs significantly from all laccases studied structurally so far...
  37. González Cerón G, Miranda Olivares O, Servín Gonzalez L. Characterization of the methyl-specific restriction system of Streptomyces coelicolor A3(2) and of the role played by laterally acquired nucleases. FEMS Microbiol Lett. 2009;301:35-43 pubmed publisher
    The methyl-specific restriction system of Streptomyces coelicolor A3(2) was analyzed by carrying out transformations with unmethylated and methylated pSET152 DNA...
  38. Wang G, Tanaka Y, Ochi K. The G243D mutation (afsB mutation) in the principal sigma factor sigmaHrdB alters intracellular ppGpp level and antibiotic production in Streptomyces coelicolor A3(2). Microbiology. 2010;156:2384-92 pubmed publisher
    Deficient antibiotic production in an afsB mutant, BH5, of Streptomyces coelicolor A3(2) was recently shown to be due to a mutation (G243D) in region 1.2 of the primary sigma factor sigma(HrdB)...
  39. Holmes N, Walshaw J, Leggett R, Thibessard A, Dalton K, Gillespie M, et al. Coiled-coil protein Scy is a key component of a multiprotein assembly controlling polarized growth in Streptomyces. Proc Natl Acad Sci U S A. 2013;110:E397-406 pubmed publisher
    ..of cell polarity for growth also requires a dedicated multiprotein assembly in the filamentous bacterium, Streptomyces coelicolor. We present evidence for a tip organizing center and confirm two of its main components: Scy (..
  40. Yang C, Glover J. The SmpB-tmRNA tagging system plays important roles in Streptomyces coelicolor growth and development. PLoS ONE. 2009;4:e4459 pubmed publisher
    ..Overall, these results suggest that the tmRNA tagging system plays important roles during Streptomyces growth and sporulation under both normal and stress conditions...
  41. de Jong W, Manteca A, Sanchez J, Bucca G, Smith C, Dijkhuizen L, et al. NepA is a structural cell wall protein involved in maintenance of spore dormancy in Streptomyces coelicolor. Mol Microbiol. 2009;71:1591-603 pubmed publisher
    ..We here compared whole genome expression of a wild-type colony of Streptomyces coelicolor forming aerial hyphae and spores with that of the chp null mutant that forms few aerial structures...
  42. Reyes A, Geukens N, Gutschoven P, De Graeve S, De Mot R, Mej a A, et al. The Streptomyces coelicolor genome encodes a type I ribosome-inactivating protein. Microbiology. 2010;156:3021-30 pubmed publisher
    ..characterization of the RIP-like gene product SCO7092 (RIPsc) from the Gram-positive soil bacterium Streptomyces coelicolor. The ripsc gene was expressed in Escherichia coli as a recombinant protein of about 30 kDa, and ..
  43. Shin J, Singh A, Cheon D, Roe J. Activation of the SoxR regulon in Streptomyces coelicolor by the extracellular form of the pigmented antibiotic actinorhodin. J Bacteriol. 2011;193:75-81 pubmed publisher
    ..The antibiotic-producing soil bacterium Streptomyces coelicolor contains a gene for an SoxR homologue (SCO1697) whose DNA recognition helix is identical to that of ..
  44. Battke F, Herbig A, Wentzel A, Jakobsen O, Bonin M, Hodgson D, et al. A technical platform for generating reproducible expression data from Streptomyces coelicolor batch cultivations. Adv Exp Med Biol. 2011;696:3-15 pubmed publisher
    b>Streptomyces coelicolor, the model species of the genus Streptomyces, presents a complex life cycle of successive morphological and biochemical changes involving the formation of substrate and aerial mycelium, sporulation and the ..
  45. Homerova D, Sevcikova B, Rezuchova B, Kormanec J. Regulation of an alternative sigma factor ?I by a partner switching mechanism with an anti-sigma factor PrsI and an anti-anti-sigma factor ArsI in Streptomyces coelicolor A3(2). Gene. 2012;492:71-80 pubmed publisher
    ..prsI and arsI, are located divergently next to the sigI gene encoding alternative sigma factor ?I of Streptomyces coelicolor A3(2)...
  46. Bucca G, Laing E, Mersinias V, Allenby N, Hurd D, Holdstock J, et al. Development and application of versatile high density microarrays for genome-wide analysis of Streptomyces coelicolor: characterization of the HspR regulon. Genome Biol. 2009;10:R5 pubmed publisher
    ..application of versatile high density ink-jet in situ-synthesized DNA arrays for the G+C rich bacterium Streptomyces coelicolor. High G+C content DNA probes often perform poorly on arrays, yielding either weak hybridization or non-..
  47. Park S, Yang Y, Song E, Kim E, Kim W, Sohng J, et al. Mass spectrometric screening of transcriptional regulators involved in antibiotic biosynthesis in Streptomyces coelicolor A3(2). J Ind Microbiol Biotechnol. 2009;36:1073-83 pubmed publisher
    ..regulators involved in the biosynthesis of actinorhodin (Act) and undecylprodigiosin (Red) in Streptomyces coelicolor. The aim of this analysis was to determine the specific transcriptional regulators binding to the ..
  48. Pawlik K, Kotowska M, Kolesinski P. Streptomyces coelicolor A3(2) produces a new yellow pigment associated with the polyketide synthase Cpk. J Mol Microbiol Biotechnol. 2010;19:147-51 pubmed publisher
    b>Streptomyces coelicolor A3(2) is an extensively studied model organism for the genetic studies of Streptomycetes - a genus known for the production of a vast number of bioactive compounds and complex regulatory networks controlling ..
  49. Dall aglio P, Arthur C, Williams C, Vasilakis K, Maple H, Crosby J, et al. Analysis of Streptomyces coelicolor phosphopantetheinyl transferase, AcpS, reveals the basis for relaxed substrate specificity. Biochemistry. 2011;50:5704-17 pubmed publisher
    ..b>Streptomyces coelicolor AcpS is a doubly promiscuous enzyme capable of activation of ACPs from both fatty acid and polyketide ..
  50. Chi W, Chang Y, Hong S. Agar degradation by microorganisms and agar-degrading enzymes. Appl Microbiol Biotechnol. 2012;94:917-30 pubmed publisher
    ..systems of two specific microorganisms: Gram-negative Saccharophagus degradans 2-40 and Gram-positive Streptomyces coelicolor A3(2)...
  51. Lee S, Mo S, Suh J. An ABC transporter complex containing S-adenosylmethionine (SAM)-induced ATP-binding protein is involved in antibiotics production and SAM signaling in Streptomyces coelicolor M145. Biotechnol Lett. 2012;34:1907-14 pubmed publisher
    A sco3956-deletion mutant (?SCO3956) of Streptomyces coelicolor was generated to characterize the S-adenosylmethionine (SAM)-induced, ATP-binding cassette transporter (ABC transporter) ATP-binding protein, SCO3956...
  52. Syson K, Stevenson C, Rashid A, Saalbach G, Tang M, Tuukkanen A, et al. Structural insight into how Streptomyces coelicolor maltosyl transferase GlgE binds ?-maltose 1-phosphate and forms a maltosyl-enzyme intermediate. Biochemistry. 2014;53:2494-504 pubmed publisher
    ..Evidence of this mechanism was obtained using a combination of site-directed mutagenesis of Streptomyces coelicolor GlgE isoform I, substrate analogues, protein crystallography, and mass spectrometry...
  53. Heggset E, Hoell I, Kristoffersen M, Eijsink V, Vårum K. Degradation of chitosans with chitinase G from Streptomyces coelicolor A3(2): production of chito-oligosaccharides and insight into subsite specificities. Biomacromolecules. 2009;10:892-9 pubmed publisher
    ..the degradation of soluble heteropolymeric chitosans with a bacterial family 19 chitinase, ChiG from Streptomyces coelicolor A3(2), to obtain insight into the mode of action of ChiG, to determine subsite preferences for acetylated ..
  54. Dong H, Jiang J, Li Y. The distinct anchoring mechanism of FtsY from different microbes. Curr Microbiol. 2009;59:336-40 pubmed publisher
    ..coli FtsY was dispensable. Moreover, the A domain of E. coli FtsY might promote itself to bind the membrane depending on the location images and Western blotting...
  55. Tunca S, Barreiro C, Coque J, Martin J. Two overlapping antiparallel genes encoding the iron regulator DmdR1 and the Adm proteins control siderophore [correction of sedephore] and antibiotic biosynthesis in Streptomyces coelicolor A3(2). FEBS J. 2009;276:4814-27 pubmed publisher
    The dmdR1 gene of Streptomyces coelicolor encodes an important regulator of iron metabolism. An antiparallel gene (adm) homologous to a development-regulated gene of Streptomyces aureofaciens has been found to overlap with dmdR1...
  56. Facey P, Sevcikova B, Novakova R, Hitchings M, Crack J, Kormanec J, et al. The dpsA gene of Streptomyces coelicolor: induction of expression from a single promoter in response to environmental stress or during development. PLoS ONE. 2011;6:e25593 pubmed publisher
    The DpsA protein plays a dual role in Streptomyces coelicolor, both as part of the stress response and contributing to nucleoid condensation during sporulation...
  57. Iqbal M, Mast Y, Amin R, Hodgson D, Wohlleben W, Burroughs N. Extracting regulator activity profiles by integration of de novo motifs and expression data: characterizing key regulators of nutrient depletion responses in Streptomyces coelicolor. Nucleic Acids Res. 2012;40:5227-39 pubmed publisher
    ..Our analysis is applicable to any organism for which there is a reasonable amount of complementary expression data and for which motifs (either over represented or evolutionary conserved) can be identified in the genome...
  58. Martin J, Santos Beneit F, Rodríguez García A, Sola Landa A, Smith M, Ellingsen T, et al. Transcriptomic studies of phosphate control of primary and secondary metabolism in Streptomyces coelicolor. Appl Microbiol Biotechnol. 2012;95:61-75 pubmed publisher
    ..The PhoP DNA-binding sequence is well characterized in Streptomyces coelicolor. It comprises at least two direct repeat units of 11 nt, the first seven of which are highly conserved...
  59. Fischer M, Schmidt C, Falke D, Sawers R. Terminal reduction reactions of nitrate and sulfate assimilation in Streptomyces coelicolor A3(2): identification of genes encoding nitrite and sulfite reductases. Res Microbiol. 2012;163:340-8 pubmed publisher
    The model actinobacterium Streptomyces coelicolor A3(2) uses nitrate and sulfate as nitrogen and sulfur sources, respectively...
  60. Wang Y, Cen X, Zhao G, Wang J. Characterization of a new GlnR binding box in the promoter of amtB in Streptomyces coelicolor inferred a PhoP/GlnR competitive binding mechanism for transcriptional regulation of amtB. J Bacteriol. 2012;194:5237-44 pubmed publisher
    The transcription of amtB in Streptomyces coelicolor has been proposed to be counter-regulated by GlnR (a global regulator for nitrogen metabolism) and PhoP (a global regulator for phosphate metabolism)...
  61. Hopkins A, Buchanan G, Palmer T. Role of the twin arginine protein transport pathway in the assembly of the Streptomyces coelicolor cytochrome bc1 complex. J Bacteriol. 2014;196:50-9 pubmed publisher
    ..In this study, we show that the activity of the Streptomyces coelicolor M145 cytochrome bc1 complex is dependent upon an active Tat pathway...
  62. Mao X, Zhou Z, Hou X, Guan W, Li Y. Reciprocal regulation between SigK and differentiation programs in Streptomyces coelicolor. J Bacteriol. 2009;191:6473-81 pubmed publisher
    Here we reported that deletion of SigK (SCO6520), a sigma factor in Streptomyces coelicolor, caused an earlier switch from vegetative mycelia to aerial mycelia and higher expression of chpE and chpH than that in the wild type...
  63. Hsiao N, Nakayama S, Merlo M, de Vries M, Bunet R, Kitani S, et al. Analysis of two additional signaling molecules in Streptomyces coelicolor and the development of a butyrolactone-specific reporter system. Chem Biol. 2009;16:951-60 pubmed publisher
    ..One gamma-butyrolactone, SCB1, has been previously characterized in Streptomyces coelicolor. Here we report the characterization of two additional gamma-butyrolactones, named SCB2 (2-[1'-..
  64. D Alia D, Nieselt K, Steigele S, Müller J, Verburg I, Takano E. Noncoding RNA of glutamine synthetase I modulates antibiotic production in Streptomyces coelicolor A3(2). J Bacteriol. 2010;192:1160-4 pubmed publisher
    ..glutamine synthetase I resulted in a decrease in growth, protein synthesis, and antibiotic production in Streptomyces coelicolor. In addition, we predicted 3,597 cis-encoded ncRNAs and validated 13 of them experimentally, including ..
  65. Aaron J, Lin X, Cane D, Christianson D. Structure of epi-isozizaene synthase from Streptomyces coelicolor A3(2), a platform for new terpenoid cyclization templates. Biochemistry. 2010;49:1787-97 pubmed publisher
    The X-ray crystal structure of recombinant epi-isozizaene synthase (EIZS), a sesquiterpene cyclase from Streptomyces coelicolor A3(2), has been determined at 1.60 A resolution...
  66. Alam M, Merlo M, Hodgson D, Wellington E, Takano E, Breitling R. Metabolic modeling and analysis of the metabolic switch in Streptomyces coelicolor. BMC Genomics. 2010;11:202 pubmed publisher
    The transition from exponential to stationary phase in Streptomyces coelicolor is accompanied by a major metabolic switch and results in a strong activation of secondary metabolism...
  67. Yang H, An Y, Wang L, Zhang S, Zhang Y, Tian Y, et al. Autoregulation of hpdR and its effect on CDA biosynthesis in Streptomyces coelicolor. Microbiology. 2010;156:2641-8 pubmed publisher
    HpdR, an IclR-family regulator in Streptomyces coelicolor, is a substrate-dependent repressor for the tyrosine catabolic gene hppD. In this study, S1 nuclease protection assays revealed that hpdR is subject to a negative autoregulation...
  68. Feng W, Mao X, Liu Z, Li Y. The ECF sigma factor SigT regulates actinorhodin production in response to nitrogen stress in Streptomyces coelicolor. Appl Microbiol Biotechnol. 2011;92:1009-21 pubmed publisher
    ..This work described the characterization of ECF sigma factor SigT in Streptomyces coelicolor. We found the absence of sigT almost abolished the production of the antibiotics actinorhodin (Act) under ..
  69. Doménech R, Martinez Rodriguez S, Velazquez Campoy A, Neira J. Peptides as inhibitors of the first phosphorylation step of the Streptomyces coelicolor phosphoenolpyruvate: sugar phosphotransferase system. Biochemistry. 2012;51:7393-402 pubmed publisher
    ..In this work, we determined the binding affinities of peptides derived from EIN of Streptomyces coelicolor (EIN(sc)) against HPr of the same organism (HPr(sc)), by using nuclear magnetic resonance and isothermal ..
  70. Saito A, Ebise H, Orihara Y, Murakami S, Sano Y, Kimura A, et al. Enzymatic and genetic characterization of the DasD protein possessing N-acetyl-?-d-glucosaminidase activity in Streptomyces coelicolor A3(2). FEMS Microbiol Lett. 2013;340:33-40 pubmed publisher
    ..an ABC transporter for uptake of a chitin-degradation product N,N'-diacetylchitobiose [(GlcNAc)(2) ] in Streptomyces coelicolor A3(2)...
  71. Santos Beneit F, Rodr guez Garc a A, Sola Landa A, Mart n J. Cross-talk between two global regulators in Streptomyces: PhoP and AfsR interact in the control of afsS, pstS and phoRP transcription. Mol Microbiol. 2009;72:53-68 pubmed publisher
    ..proteins AfsR and PhoP control expression of the biosynthesis of actinorhodin and undecylprodigiosin in Streptomyces coelicolor. Electrophoretic mobility shift assays showed that PhoP(DBD) does not bind directly to the actII-ORF4, ..
  72. Gao B, Sugiman Marangos S, Junop M, Gupta R. Structural and phylogenetic analysis of a conserved actinobacteria-specific protein (ASP1; SCO1997) from Streptomyces coelicolor. BMC Struct Biol. 2009;9:40 pubmed publisher
    ..the first characterization of one of the 5 actinobacteria-specific proteins, ASP1 (Gene ID: SCO1997) from Streptomyces coelicolor. The X-ray crystal structure of ASP1 was determined at 2.2 A...
  73. Facey P, Hitchings M, Saavedra Garcia P, Fernandez Martinez L, Dyson P, Del Sol R. Streptomyces coelicolor Dps-like proteins: differential dual roles in response to stress during vegetative growth and in nucleoid condensation during reproductive cell division. Mol Microbiol. 2009;73:1186-202 pubmed publisher
    ..revealed the presence of three Dps-like orthologues within the genome of the Gram-positive bacterium Streptomyces coelicolor. Disruption of the S...
  74. Kallifidas D, Thomas D, Doughty P, Paget M. The sigmaR regulon of Streptomyces coelicolor A32 reveals a key role in protein quality control during disulphide stress. Microbiology. 2010;156:1661-72 pubmed publisher
    ..The Gram-positive bacterium Streptomyces coelicolor senses and responds to disulphide stress through the sigma(R)-RsrA system, which comprises an ..
  75. Parker J, Jones A, Serazetdinova L, Saalbach G, Bibb M, Naldrett M. Analysis of the phosphoproteome of the multicellular bacterium Streptomyces coelicolor A3(2) by protein/peptide fractionation, phosphopeptide enrichment and high-accuracy mass spectrometry. Proteomics. 2010;10:2486-97 pubmed publisher
    The serine (Ser)/threonine (Thr)/tyrosine (Tyr) phosphoproteome of exponentially growing Streptomyces coelicolor A3(2) was analysed using the gel-free approaches of preparative IEF for protein fractionation, followed by strong cation ..
  76. Tanaka Y, Hosaka T, Ochi K. Rare earth elements activate the secondary metabolite-biosynthetic gene clusters in Streptomyces coelicolor A3(2). J Antibiot (Tokyo). 2010;63:477-81 pubmed publisher
    ..the expression of nine genes belonging to nine secondary metabolite-biosynthetic gene clusters of Streptomyces coelicolor A3(2) when added to the medium at low concentrations...
  77. Heichlinger A, Ammelburg M, Kleinschnitz E, Latus A, Maldener I, Fl rdh K, et al. The MreB-like protein Mbl of Streptomyces coelicolor A3(2) depends on MreB for proper localization and contributes to spore wall synthesis. J Bacteriol. 2011;193:1533-42 pubmed publisher
    ..In contrast, MreB of Streptomyces coelicolor is not required for vegetative growth but has a role in sporulation. Besides MreB, S...
  78. Murakami T, Burian J, Yanai K, Bibb M, Thompson C. A system for the targeted amplification of bacterial gene clusters multiplies antibiotic yield in Streptomyces coelicolor. Proc Natl Acad Sci U S A. 2011;108:16020-5 pubmed publisher
    ..ZouA-mediated DNA amplification promises to be a valuable tool for increasing the activities of commercially important biosynthetic, degradative, and photosynthetic pathways in a wide variety of organisms...
  79. Bielen A, Cetkovic H, Long P, Schwab H, Abramić M, Vujaklija D. The SGNH-hydrolase of Streptomyces coelicolor has (aryl)esterase and a true lipase activity. Biochimie. 2009;91:390-400 pubmed publisher
    The Streptomyces coelicolor A3(2) gene SCI11.14c was overexpressed and purified as a His-tagged protein from heterologous host, Streptomyces lividans. The purification procedure resulted in 34...
  80. Santos Beneit F, Rodr guez Garc a A, Apel A, Mart n J. Phosphate and carbon source regulation of two PhoP-dependent glycerophosphodiester phosphodiesterase genes of Streptomyces coelicolor. Microbiology. 2009;155:1800-11 pubmed publisher
    Glycerophosphodiesters are formed by deacylation of phospholipids. Streptomyces coelicolor and other soil-dwelling actinomycetes utilize glycerophosphodiesters as phosphate and carbon sources by the action of glycerophosphodiester ..
  81. Heggset E, Dybvik A, Hoell I, Norberg A, Sørlie M, Eijsink V, et al. Degradation of chitosans with a family 46 chitosanase from Streptomyces coelicolor A3(2). Biomacromolecules. 2010;11:2487-97 pubmed publisher
    ..of well-characterized soluble heteropolymeric chitosans by a novel family 46 chitosanase, ScCsn46A from Streptomyces coelicolor A3(2), to obtain insight into the enzyme's mode of action and to determine its potential for production ..
  82. Xu D, Seghezzi N, Esnault C, Virolle M. Repression of antibiotic production and sporulation in Streptomyces coelicolor by overexpression of a TetR family transcriptional regulator. Appl Environ Microbiol. 2010;76:7741-53 pubmed publisher
    ..of a regulatory gene of the TetR family (SCO3201) originating either from Streptomyces lividans or from Streptomyces coelicolor was shown to strongly repress antibiotic production (calcium-dependent antibiotic [CDA], ..
  83. Tiffert Y, Franz Wachtel M, Fladerer C, Nordheim A, Reuther J, Wohlleben W, et al. Proteomic analysis of the GlnR-mediated response to nitrogen limitation in Streptomyces coelicolor M145. Appl Microbiol Biotechnol. 2011;89:1149-59 pubmed publisher
    GlnR is the global regulator of nitrogen assimilation in Streptomyces coelicolor M145 and other actinobacteria...
  84. Takano H, Hashimoto K, Yamamoto Y, Beppu T, Ueda K. Pleiotropic effect of a null mutation in the cvn1 conservon of Streptomyces coelicolor A3(2). Gene. 2011;477:12-8 pubmed publisher
    ..present study, we generated null mutants for all 13 copies of the cvn operon distributed in the genome of Streptomyces coelicolor A3(2) and observed that the aerial mycelium formation and antibiotic production in a cvn1 mutant were ..
  85. Mikulík K, Felsberg J, Kudrn ov E, Bezou kov S, Setinov D, Stod lkov E, et al. CobB1 deacetylase activity in Streptomyces coelicolor. Biochem Cell Biol. 2012;90:179-87 pubmed publisher
    ..This report provides first evidence for deacetylation activity of CobB1 in Streptomyces coelicolor. The protein is highly conserved in streptomycetes...
  86. Hull T, Ryu M, Sullivan M, Johnson R, Klena N, Geiger R, et al. Cyclic Di-GMP phosphodiesterases RmdA and RmdB are involved in regulating colony morphology and development in Streptomyces coelicolor. J Bacteriol. 2012;194:4642-51 pubmed publisher
    ..characterize two c-di-GMP phosphodiesterases from the filamentous high-GC Gram-positive actinobacterium Streptomyces coelicolor, involved in controlling colony morphology and development...
  87. Swiatek M, Gubbens J, Bucca G, Song E, Yang Y, Laing E, et al. The ROK family regulator Rok7B7 pleiotropically affects xylose utilization, carbon catabolite repression, and antibiotic production in streptomyces coelicolor. J Bacteriol. 2013;195:1236-48 pubmed publisher
    ..rok7B7) strongly affects carbon catabolite repression (CCR), growth, and antibiotic production in Streptomyces coelicolor. Deletion of SCO7543 also affected antibiotic production, while no major changes were observed after ..
  88. Persson J, Chater K, Fl rdh K. Molecular and cytological analysis of the expression of Streptomyces sporulation regulatory gene whiH. FEMS Microbiol Lett. 2013;341:96-105 pubmed publisher
    The whiH gene is required for the orderly sporulation septation that divides aerial hyphae into spores in Streptomyces coelicolor. Here, we use a whiHp-mCherry transcriptional reporter construct to show that whiHp is active specifically ..
  89. Hayashi T, Tanaka Y, Sakai N, Okada U, Yao M, Watanabe N, et al. SCO4008, a putative TetR transcriptional repressor from Streptomyces coelicolor A3(2), regulates transcription of sco4007 by multidrug recognition. J Mol Biol. 2013;425:3289-300 pubmed publisher
    SCO4008 from Streptomyces coelicolor A3(2) is a member of the TetR family. However, its precise function is not yet clear. In this study, the crystal structure of SCO4008 was determined at a resolution of 2...
  90. Yeo K, Han Y, Eo Y, Cheong H. Expression, purification, crystallization and preliminary X-ray analysis of the extracellular sensory domain of DraK histidine kinase from Streptomyces coelicolor. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013;69:909-11 pubmed publisher
    The bacterium Streptomyces coelicolor produces useful antibiotics from its secondary metabolites. DraK is a sensory histidine kinase involved in the differential regulation of antibiotics in S...
  91. Soror S, Rao R, Cullum J. Mining the genome sequence for novel enzyme activity: characterisation of an unusual member of the hormone-sensitive lipase family of esterases from the genome of Streptomyces coelicolor A3 (2). Protein Eng Des Sel. 2009;22:333-9 pubmed publisher
    ..The location of conserved amino acid motifs in a 3-D homology model of the enzyme identified further members of the family with unusual amino acid replacements...
  92. Lin X, Cane D. Biosynthesis of the sesquiterpene antibiotic albaflavenone in Streptomyces coelicolor. Mechanism and stereochemistry of the enzymatic formation of epi-isozizaene. J Am Chem Soc. 2009;131:6332-3 pubmed publisher
    Epi-isozizaene synthase from Streptomyces coelicolor catalyzes the multistep cyclization of farnesyl diphosphate (2, FPP) to the tricyclic sesquiterpene hydrocarbon (+)-epi-isozizaene (3), which is converted in turn to the antibiotic ..