clostridium thermocellum

Summary

Summary: A species of gram-positive, thermophilic, cellulolytic bacteria in the family Clostridaceae. It degrades and ferments CELLOBIOSE and CELLULOSE to ETHANOL in the CELLULOSOME.

Top Publications

  1. Zhang Y, Lynd L. Cellulose utilization by Clostridium thermocellum: bioenergetics and hydrolysis product assimilation. Proc Natl Acad Sci U S A. 2005;102:7321-5 pubmed
    The bioenergetics of cellulose utilization by Clostridium thermocellum was investigated. Cell yield and maintenance parameters, Y(X/ATP)True = 16.44 g cell/mol ATP and m = 3...
  2. Xu J, Smith J. Probing the mechanism of cellulosome attachment to the Clostridium thermocellum cell surface: computer simulation of the Type II cohesin-dockerin complex and its variants. Protein Eng Des Sel. 2010;23:759-68 pubmed publisher
    ..Efficient degradation of biomass by the anaerobic bacterium Clostridium thermocellum is carried out by the multicomponent cellulosome complex...
  3. Fan Z, Werkman J, Yuan L. Engineering of a multifunctional hemicellulase. Biotechnol Lett. 2009;31:751-7 pubmed publisher
    ..a multifunctional xylan-degrading enzyme, a chimera was created by fusing the xylanase domain of the Clostridium thermocellum xylanase (xynZ) and a dual functional arabinofuranosidase/xylosidase (DeAFc; from a compost starter ..
  4. Takishita K, Inagaki Y. Eukaryotic origin of glyceraldehyde-3-phosphate dehydrogenase genes in Clostridium thermocellum and Clostridium cellulolyticum genomes and putative fates of the exogenous gene in the subsequent genome evolution. Gene. 2009;441:22-7 pubmed publisher
    ..A series of phylogenetic analyses of GAPDH homologues revealed that Clostridium thermocellum and Clostridium cellulolyticum homologues have the evolutionary affinity to the eukaryotic homologues, ..
  5. Kahel Raifer H, Jindou S, Bahari L, Nataf Y, Shoham Y, Bayer E, et al. The unique set of putative membrane-associated anti-sigma factors in Clostridium thermocellum suggests a novel extracellular carbohydrate-sensing mechanism involved in gene regulation. FEMS Microbiol Lett. 2010;308:84-93 pubmed publisher
    Genome analysis of the Gram-positive cellulolytic bacterium Clostridium thermocellum revealed the presence of multiple negative regulators of alternative sigma factors...
  6. Richter H, Zoephel J, Schermuly J, Maticzka D, Backofen R, Randau L. Characterization of CRISPR RNA processing in Clostridium thermocellum and Methanococcus maripaludis. Nucleic Acids Res. 2012;40:9887-96 pubmed publisher
    ..I-B and present the identification of novel Cas6 enzymes from the bacterial and archaeal model organisms Clostridium thermocellum and Methanococcus maripaludis C5...
  7. Pinheiro B, Gilbert H, Sakka K, Sakka K, Fernandes V, Prates J, et al. Functional insights into the role of novel type I cohesin and dockerin domains from Clostridium thermocellum. Biochem J. 2009;424:375-84 pubmed publisher
    Cellulosomes, synthesized by anaerobic microorganisms such as Clostridium thermocellum, are remarkably complex nanomachines that efficiently degrade plant cell wall polysaccharides...
  8. Vazana Y, Moraïs S, Barak Y, Lamed R, Bayer E. Interplay between Clostridium thermocellum family 48 and family 9 cellulases in cellulosomal versus noncellulosomal states. Appl Environ Microbiol. 2010;76:3236-43 pubmed publisher
    The anaerobic, thermophilic cellulolytic bacterium Clostridium thermocellum is known for its elaborate cellulosome complex, but it also produces a separate free cellulase system...
  9. Riederer A, Takasuka T, Makino S, Stevenson D, Bukhman Y, Elsen N, et al. Global gene expression patterns in Clostridium thermocellum as determined by microarray analysis of chemostat cultures on cellulose or cellobiose. Appl Environ Microbiol. 2011;77:1243-53 pubmed publisher
    ..chemostat growth on insoluble cellulose or soluble cellobiose has provided substantial new information on Clostridium thermocellum gene expression. This is the first comprehensive examination of gene expression in C...

More Information

Publications93

  1. Fierobe H, Mingardon F, Mechaly A, Belaich A, Rincon M, Pagès S, et al. Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined trifunctional scaffoldin. J Biol Chem. 2005;280:16325-34 pubmed
    ..Furthermore cooperation within a cellulosome chimera between cellulases and a hemicellulase from different microorganisms was achieved, leading to a trifunctional complex with enhanced activity on a complex substrate...
  2. Ribeiro T, Lordelo M, Prates J, Falcao L, Freire J, Ferreira L, et al. The thermostable ?-1,3-1,4-glucanase from Clostridium thermocellum improves the nutritive value of highly viscous barley-based diets for broilers. Br Poult Sci. 2012;53:224-34 pubmed publisher
    ..3. Here we describe the biochemical properties of the family 16 GH from Clostridium thermocellum, termed CtGlc16A...
  3. Bahari L, Gilad Y, Borovok I, Kahel Raifer H, Dassa B, Nataf Y, et al. Glycoside hydrolases as components of putative carbohydrate biosensor proteins in Clostridium thermocellum. J Ind Microbiol Biotechnol. 2011;38:825-32 pubmed publisher
    The composition of the cellulase system in the cellulosome-producing bacterium, Clostridium thermocellum, has been reported to change in response to growth on different carbon sources...
  4. Nataf Y, Bahari L, Kahel Raifer H, Borovok I, Lamed R, Bayer E, et al. Clostridium thermocellum cellulosomal genes are regulated by extracytoplasmic polysaccharides via alternative sigma factors. Proc Natl Acad Sci U S A. 2010;107:18646-51 pubmed publisher
    b>Clostridium thermocellum produces a highly efficient cellulolytic extracellular complex, termed the cellulosome, for hydrolyzing plant cell wall biomass...
  5. Brown S, Guss A, Karpinets T, Parks J, Smolin N, Yang S, et al. Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum. Proc Natl Acad Sci U S A. 2011;108:13752-7 pubmed publisher
    b>Clostridium thermocellum is a thermophilic, obligately anaerobic, gram-positive bacterium that is a candidate microorganism for converting cellulosic biomass into ethanol through consolidated bioprocessing...
  6. Martins A, Shuman S. An end-healing enzyme from Clostridium thermocellum with 5' kinase, 2',3' phosphatase, and adenylyltransferase activities. RNA. 2005;11:1271-80 pubmed
    We identify and characterize an end-healing enzyme, CthPnkp, from Clostridium thermocellum that catalyzes the phosphorylation of 5'-OH termini of DNA or RNA polynucleotides and the dephosphorylation of 2',3' cyclic phosphate, 2'-phosphate,..
  7. Newcomb M, Millen J, Chen C, Wu J. Co-transcription of the celC gene cluster in Clostridium thermocellum. Appl Microbiol Biotechnol. 2011;90:625-34 pubmed publisher
    b>Clostridium thermocellum, an anaerobic, thermophilic, and ethanogenic bacterium produces a large cellulase complex termed the cellulosome and many free glycosyl hydrolases. Most cellulase genes scatter around the genome...
  8. Yoon R, Yeom S, Kim H, Oh D. Novel substrates of a ribose-5-phosphate isomerase from Clostridium thermocellum. J Biotechnol. 2009;139:26-32 pubmed publisher
    A substrate specificity study of the recombinant D-ribose-5-phosphate isomerase (RpiB) from Clostridium thermocellum was performed...
  9. Petersen L, Ardèvol A, Rovira C, Reilly P. Mechanism of cellulose hydrolysis by inverting GH8 endoglucanases: a QM/MM metadynamics study. J Phys Chem B. 2009;113:7331-9 pubmed publisher
    ..In this work, hydrolysis by Clostridium thermocellum endo-1,4-glucanase A was computationally simulated with quantum mechanics/molecular mechanics ..
  10. Keppetipola N, Jain R, Shuman S. Novel triphosphate phosphohydrolase activity of Clostridium thermocellum TTM, a member of the triphosphate tunnel metalloenzyme superfamily. J Biol Chem. 2007;282:11941-9 pubmed publisher
    ..have conducted a biochemical and structure-function analysis of a TTM protein (CthTTM) from the bacterium Clostridium thermocellum. CthTTM is a metal-dependent tripolyphosphatase and nucleoside triphosphatase; it is not an adenylate ..
  11. Keppetipola N, Shuman S. Distinct enzymic functional groups are required for the phosphomonoesterase and phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase/phosphatase. J Biol Chem. 2006;281:19251-9 pubmed
    The central phosphatase domain of Clostridium thermocellum polynucleotide kinase/phosphatase (CthPnkp) belongs to the dinuclear metallophosphoesterase superfamily...
  12. Keppetipola N, Shuman S. Mechanism of the phosphatase component of Clostridium thermocellum polynucleotide kinase-phosphatase. RNA. 2006;12:73-82 pubmed
    Polynucleotide kinase-phosphatase (Pnkp) from Clostridium thermocellum catalyzes ATP-dependent phosphorylation of 5'-OH termini of DNA or RNA polynucleotides and Ni(2+)/Mn(2+)-dependent dephosphorylation of 2',3' cyclic phosphate, 2'-..
  13. Dvortsov I, Lunina N, Chekanovskaya L, Schwarz W, Zverlov V, Velikodvorskaya G. Carbohydrate-binding properties of a separately folding protein module from beta-1,3-glucanase Lic16A of Clostridium thermocellum. Microbiology. 2009;155:2442-9 pubmed publisher
    The multi-modular non-cellulosomal endo-1,3(4)-beta-glucanase Lic16A from Clostridium thermocellum contains a so-called X module (denoted as CBMX) near the N terminus of the catalytic module (191-426 aa)...
  14. Yang S, Giannone R, Dice L, Yang Z, Engle N, Tschaplinski T, et al. Clostridium thermocellum ATCC27405 transcriptomic, metabolomic and proteomic profiles after ethanol stress. BMC Genomics. 2012;13:336 pubmed publisher
    b>Clostridium thermocellum is a candidate consolidated bioprocessing biocatalyst, which is a microorganism that expresses enzymes for both cellulose hydrolysis and its fermentation to produce fuels such as lignocellulosic ethanol...
  15. Gold N, Martin V. Global view of the Clostridium thermocellum cellulosome revealed by quantitative proteomic analysis. J Bacteriol. 2007;189:6787-95 pubmed
    ..alterations in the expression patterns of subunits within cellulosomes of the cellulolytic bacterium Clostridium thermocellum, grown on either cellulose or cellobiose...
  16. Olson D, Tripathi S, Giannone R, Lo J, Caiazza N, Hogsett D, et al. Deletion of the Cel48S cellulase from Clostridium thermocellum. Proc Natl Acad Sci U S A. 2010;107:17727-32 pubmed publisher
    b>Clostridium thermocellum is a thermophilic anaerobic bacterium that rapidly solubilizes cellulose with the aid of a multienzyme cellulosome complex...
  17. Linville J, Rodriguez M, Mielenz J, Cox C. Kinetic modeling of batch fermentation for Populus hydrolysate tolerant mutant and wild type strains of Clostridium thermocellum. Bioresour Technol. 2013;147:605-13 pubmed publisher
    The extent of inhibition of two strains of Clostridium thermocellum by a Populus hydrolysate was investigated. A Monod-based model of wild type (WT) and Populus hydrolysate tolerant mutant (PM) strains of the cellulolytic bacterium C...
  18. Yu T, Xu X, Peng Y, Luo Y, Yang K. Cell wall proteome of Clostridium thermocellum and detection of glycoproteins. Microbiol Res. 2012;167:364-71 pubmed publisher
    b>Clostridium thermocellum, a thermophilic anaerobe, has the unusual capacity to convert cellulosic biomass into ethanol and hydrogen. In this work, the cell wall proteome of C. thermocellum was investigated...
  19. McBee R. The characteristics of Clostridium thermocellum. J Bacteriol. 1954;67:505-6 pubmed
  20. Gowen C, Fong S. Genome-scale metabolic model integrated with RNAseq data to identify metabolic states of Clostridium thermocellum. Biotechnol J. 2010;5:759-67 pubmed publisher
    ..we generated RNA sequencing data to characterize the transcriptional state of the cellulolytic anaerobe, Clostridium thermocellum, and algorithmically integrated these data with a genome-scale metabolic model...
  21. Jindou S, Xu Q, Kenig R, Shulman M, Shoham Y, Bayer E, et al. Novel architecture of family-9 glycoside hydrolases identified in cellulosomal enzymes of Acetivibrio cellulolyticus and Clostridium thermocellum. FEMS Microbiol Lett. 2006;254:308-16 pubmed
    ..An identical modular arrangement exists in two putative GH9 genes from the draft sequence of the Clostridium thermocellum genome. The three homologous CBM3b modules from A. cellulolyticus and C...
  22. Zverlov V, Schantz N, Schmitt Kopplin P, Schwarz W. Two new major subunits in the cellulosome of Clostridium thermocellum: xyloglucanase Xgh74A and endoxylanase Xyn10D. Microbiology. 2005;151:3395-401 pubmed
    ..of xyloglucanase Xgh74A and endoxylanase Xyn10D, components in the cellulosomes of cellulose-grown Clostridium thermocellum, were determined...
  23. Peng J, Wang W, Jiang Y, Liu M, Zhang H, Shao W. Enhanced soluble expression of a thermostable cellulase from Clostridium thermocellum in Escherichia coli. Curr Microbiol. 2011;63:523-30 pubmed publisher
    In this study, the cellulase gene celD from Clostridium thermocellum was cloned into expression vectors pET-20b(+) and pHsh...
  24. Ichinose H, Kuno A, Kotake T, Yoshida M, Sakka K, Hirabayashi J, et al. Characterization of an exo-beta-1,3-galactanase from Clostridium thermocellum. Appl Environ Microbiol. 2006;72:3515-23 pubmed
    A gene encoding an exo-beta-1,3-galactanase from Clostridium thermocellum, Ct1,3Gal43A, was isolated. The sequence has similarity with an exo-beta-1,3-galactanase of Phanerochaete chrysosporium (Pc1,3Gal43A)...
  25. Yi Z, Pei X, Wu Z. Introduction of glycine and proline residues onto protein surface increases the thermostability of endoglucanase CelA from Clostridium thermocellum. Bioresour Technol. 2011;102:3636-8 pubmed publisher
    A saturation mutagenesis library was constructed at the position 329 of the endoglucanase CelA from Clostridium thermocellum based on previous results (Yi and Wu, 2010), and one mutation, S329G, was identified to contribute to the ..
  26. Ye X, Zhang C, Zhang Y. Engineering a large protein by combined rational and random approaches: stabilizing the Clostridium thermocellum cellobiose phosphorylase. Mol Biosyst. 2012;8:1815-23 pubmed publisher
    The Clostridium thermocellum cellobiose phosphorylase (CtCBP) is a large protein consisting of 812 amino acids and has great potential in the production of sugar phosphates, novel glycosides, and biofuels...
  27. Nataf Y, Yaron S, Stahl F, Lamed R, Bayer E, Scheper T, et al. Cellodextrin and laminaribiose ABC transporters in Clostridium thermocellum. J Bacteriol. 2009;191:203-9 pubmed publisher
    b>Clostridium thermocellum is an anaerobic thermophilic bacterium that grows efficiently on cellulosic biomass...
  28. Kridelbaugh D, Nelson J, Engle N, Tschaplinski T, Graham D. Nitrogen and sulfur requirements for Clostridium thermocellum and Caldicellulosiruptor bescii on cellulosic substrates in minimal nutrient media. Bioresour Technol. 2013;130:125-35 pubmed publisher
    Growth media for cellulolytic Clostridium thermocellum ATCC 27405 and Caldicellulosiruptor bescii bacteria usually contain excess nutrients that would increase costs for consolidated bioprocessing for biofuel production and create a waste ..
  29. Zverlov V, Klupp M, Krauss J, Schwarz W. Mutations in the scaffoldin gene, cipA, of Clostridium thermocellum with impaired cellulosome formation and cellulose hydrolysis: insertions of a new transposable element, IS1447, and implications for cellulase synergism on crystalline cellulose. J Bacteriol. 2008;190:4321-7 pubmed publisher
    Mutants of Clostridium thermocellum that had lost the ability to adhere to microcrystalline cellulose were isolated. Six of them that showed diminished ability to depolymerize crystalline cellulose were selected...
  30. HUDSON A, Klartag A, Gilvarg C, Dobson R, Marques F, Leustek T. Dual diaminopimelate biosynthesis pathways in Bacteroides fragilis and Clostridium thermocellum. Biochim Biophys Acta. 2011;1814:1162-8 pubmed publisher
    Bacteroides fragilis and Clostridium thermocellum were recently found to synthesize diaminopimelate (DAP) by way of LL-DAP aminotransferase...
  31. Brown S, Lamed R, Morag E, Borovok I, Shoham Y, Klingeman D, et al. Draft genome sequences for Clostridium thermocellum wild-type strain YS and derived cellulose adhesion-defective mutant strain AD2. J Bacteriol. 2012;194:3290-1 pubmed publisher
    b>Clostridium thermocellum wild-type strain YS is an anaerobic, thermophilic, cellulolytic bacterium capable of directly converting cellulosic substrates into ethanol...
  32. Zhang Y, Lynd L. Regulation of cellulase synthesis in batch and continuous cultures of Clostridium thermocellum. J Bacteriol. 2005;187:99-106 pubmed
    ..cellulase synthesis (expressed in milligrams of cellulase per gram [dry weight] of cells) by Clostridium thermocellum was investigated using an enzyme-linked immunosorbent assay protocol based on antibody raised against a ..
  33. Baek S, Kim S, Lee K, Lee J, Hahn J. Cellulosic ethanol production by combination of cellulase-displaying yeast cells. Enzyme Microb Technol. 2012;51:366-72 pubmed publisher
    ..Thermoascus aurantiacus EGI and Trichoderma reesei EGII, and two bacterial endoglucanases, Clostridium thermocellum CelA and CelD, were expressed on the yeast surface, and their surface expression levels, pH- and ..
  34. Williams T, Combs J, Thakur A, Strobel H, Lynn B. A novel Bicine running buffer system for doubled sodium dodecyl sulfate - polyacrylamide gel electrophoresis of membrane proteins. Electrophoresis. 2006;27:2984-95 pubmed
    ..Membrane proteins from the anaerobic bacterium Clostridium thermocellum were used for these investigations...
  35. Petkun S, Jindou S, Shimon L, Rosenheck S, Bayer E, Lamed R, et al. Structure of a family 3b' carbohydrate-binding module from the Cel9V glycoside hydrolase from Clostridium thermocellum: structural diversity and implications for carbohydrate binding. Acta Crystallogr D Biol Crystallogr. 2010;66:33-43 pubmed publisher
    ..In particular, CBM3b' from Clostridium thermocellum Cel9V exhibits such sequence changes and fails to bind cellulosic substrates...
  36. Karpol A, Barak Y, Lamed R, Shoham Y, Bayer E. Functional asymmetry in cohesin binding belies inherent symmetry of the dockerin module: insight into cellulosome assembly revealed by systematic mutagenesis. Biochem J. 2008;410:331-8 pubmed
    ..by progressive truncation and alanine scanning of a representative type-I dockerin module from Clostridium thermocellum, that only one of the repeated motifs is critical for high-affinity cohesin binding...
  37. Keppetipola N, Shuman S. Characterization of the 2',3' cyclic phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase-phosphatase and bacteriophage lambda phosphatase. Nucleic Acids Res. 2007;35:7721-32 pubmed
    b>Clostridium thermocellum polynucleotide kinase-phosphatase (CthPnkp) catalyzes 5' and 3' end-healing reactions that prepare broken RNA termini for sealing by RNA ligase...
  38. Park C, Yeom S, Kim H, Lee S, Lee J, Kim S, et al. Characterization of ribose-5-phosphate isomerase of Clostridium thermocellum producing D-allose from D-psicose. Biotechnol Lett. 2007;29:1387-91 pubmed
    The rpiB gene, encoding ribose-5-phosphate isomerase (RpiB) from Clostridium thermocellum, was cloned and expressed in Escherichia coli...
  39. Liu Z, Bartlow P, Dilmore R, Soong Y, Pan Z, Koepsel R, et al. Production, purification, and characterization of a fusion protein of carbonic anhydrase from Neisseria gonorrhoeae and cellulose binding domain from Clostridium thermocellum. Biotechnol Prog. 2009;25:68-74 pubmed publisher
    ..bifunctional enzyme containing CA from Neisseria gonorrhoeae and a cellulose binding domain (CBD) from Clostridium thermocellum was constructed with a His6 tag. The chimeric enzyme exhibited both CA activity and CBD binding affinity...
  40. Lu Y, Zhang Y, Lynd L. Enzyme-microbe synergy during cellulose hydrolysis by Clostridium thermocellum. Proc Natl Acad Sci U S A. 2006;103:16165-9 pubmed
    ..7- to 4.7-fold) for growing cultures of Clostridium thermocellum as compared with purified cellulase preparations from this organism in controlled experiments involving ..
  41. Wang L, Das U, Smith P, Shuman S. Structure and mechanism of the polynucleotide kinase component of the bacterial Pnkp-Hen1 RNA repair system. RNA. 2012;18:2277-86 pubmed publisher
    ..Here we report the crystal structure of the kinase domain of Clostridium thermocellum Pnkp bound to ATP•Mg²? (substrate complex) and ADP•Mg²? (product complex)...
  42. Islam R, Cicek N, Sparling R, Levin D. Influence of initial cellulose concentration on the carbon flow distribution during batch fermentation by Clostridium thermocellum ATCC 27405. Appl Microbiol Biotechnol. 2009;82:141-8 pubmed publisher
    ..hydrogen (H(2)) synthesis and metabolic flow patterns in the thermophilic, cellulolytic bacterium, Clostridium thermocellum. C...
  43. Zhao G, Li H, Wamalwa B, Sakka M, Kimura T, Sakka K. Different binding specificities of S-layer homology modules from Clostridium thermocellum AncA, Slp1, and Slp2. Biosci Biotechnol Biochem. 2006;70:1636-41 pubmed
    S-layer homology (SLH) module polypeptides were derived from Clostridium thermocellum S-layer proteins Slp1 and Slp2 and cellulosome anchoring protein AncA as rSlp1-SLH, rSlp2-SLH, and rAncA-SLH respectively...
  44. Mohr G, Hong W, Zhang J, Cui G, Yang Y, Cui Q, et al. A targetron system for gene targeting in thermophiles and its application in Clostridium thermocellum. PLoS ONE. 2013;8:e69032 pubmed publisher
  45. Smith P, Wang L, Nair P, Shuman S. The adenylyltransferase domain of bacterial Pnkp defines a unique RNA ligase family. Proc Natl Acad Sci U S A. 2012;109:2296-301 pubmed publisher
    ..and evolution of this repair system, we determined the crystal structures of the ligase domain of Clostridium thermocellum Pnkp in three functional states along the reaction pathway: apoenzyme, ligase • ATP substrate complex, ..
  46. Jeon E, Hyeon J, Suh D, Suh Y, Kim S, Song K, et al. Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera beta-glucosidase genes. Mol Cells. 2009;28:369-73 pubmed publisher
    Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and beta-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an alpha-mating factor signal peptide ..
  47. Zverlov V, Schantz N, Schwarz W. A major new component in the cellulosome of Clostridium thermocellum is a processive endo-beta-1,4-glucanase producing cellotetraose. FEMS Microbiol Lett. 2005;249:353-8 pubmed
    Cel9R, a major component in the cellulosome of Clostridium thermocellum, is one of the most prevalent beta-glucanases in the complex after Cel48S and Cel8A. The recombinant product of gene celR is optimally active at 78...
  48. Brás J, Cartmell A, Carvalho A, Verzé G, Bayer E, Vazana Y, et al. Structural insights into a unique cellulase fold and mechanism of cellulose hydrolysis. Proc Natl Acad Sci U S A. 2011;108:5237-42 pubmed publisher
    b>Clostridium thermocellum is a well-characterized cellulose-degrading microorganism. The genome sequence of C...
  49. Hyeon J, Yu K, Suh D, Suh Y, Lee S, Lee J, et al. Production of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae. FEMS Microbiol Lett. 2010;310:39-47 pubmed publisher
    ..of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C...
  50. Rydzak T, McQueen P, Krokhin O, Spicer V, Ezzati P, Dwivedi R, et al. Proteomic analysis of Clostridium thermocellum core metabolism: relative protein expression profiles and growth phase-dependent changes in protein expression. BMC Microbiol. 2012;12:214 pubmed publisher
    b>Clostridium thermocellum produces H2 and ethanol, as well as CO2, acetate, formate, and lactate, directly from cellulosic biomass. It is therefore an attractive model for biofuel production via consolidated bioprocessing...
  51. Tripathi S, Olson D, Argyros D, Miller B, Barrett T, Murphy D, et al. Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant. Appl Environ Microbiol. 2010;76:6591-9 pubmed publisher
    We report development of a genetic system for making targeted gene knockouts in Clostridium thermocellum, a thermophilic anaerobic bacterium that rapidly solubilizes cellulose...
  52. Rydzak T, Levin D, Cicek N, Sparling R. Growth phase-dependant enzyme profile of pyruvate catabolism and end-product formation in Clostridium thermocellum ATCC 27405. J Biotechnol. 2009;140:169-75 pubmed publisher
    ..5), and stationary phase (OD(600) approximately 0.7) cell extracts were determined in Clostridium thermocellum ATCC 27405 grown in batch cultures on cellobiose...
  53. Dumitrache A, Wolfaardt G, Allen G, Liss S, Lynd L. Form and function of Clostridium thermocellum biofilms. Appl Environ Microbiol. 2013;79:231-9 pubmed publisher
    ..formation, and carbon flow associated with biofilms of the obligately anaerobic cellulolytic bacterium Clostridium thermocellum 27405...
  54. Magnusson L, Cicek N, Sparling R, Levin D. Continuous hydrogen production during fermentation of alpha-cellulose by the thermophillic bacterium Clostridium thermocellum. Biotechnol Bioeng. 2009;102:759-66 pubmed publisher
    ..during fermentation of alpha-cellulose was established using the thermophillic, anaerobic bacterium Clostridium thermocellum ATCC 27405. The objectives of this work were to characterize growth of C...
  55. van der Veen D, Lo J, Brown S, Johnson C, Tschaplinski T, Martin M, et al. Characterization of Clostridium thermocellum strains with disrupted fermentation end-product pathways. J Ind Microbiol Biotechnol. 2013;40:725-34 pubmed publisher
    b>Clostridium thermocellum is a thermophilic, cellulolytic anaerobe that is a candidate microorganism for industrial biofuels production...
  56. Zhu X, Cui J, Feng Y, Fa Y, Zhang J, Cui Q. Metabolic adaption of ethanol-tolerant Clostridium thermocellum. PLoS ONE. 2013;8:e70631 pubmed publisher
    b>Clostridium thermocellum is a major candidate for bioethanol production via consolidated bioprocessing. However, the low ethanol tolerance of the organism dramatically impedes its usage in industry...
  57. Selvaraj T, Kim S, Kim Y, Jeong Y, Kim Y, Phuong N, et al. The role of carbohydrate-binding module (CBM) repeat of a multimodular xylanase (XynX) from Clostridium thermocellum in cellulose and xylan binding. J Microbiol. 2010;48:856-61 pubmed publisher
    A non-cellulosomal xylanase from Clostridium thermocellum, XynX, consists of a family-22 carbohydratebinding module (CBM22), a family-10 glycoside hydrolase (GH10) catalytic module, two family-9 carbohydrate-binding modules (CBM9-I and ..
  58. Adams J, Webb B, Spencer H, Smith S. Structural characterization of type II dockerin module from the cellulosome of Clostridium thermocellum: calcium-induced effects on conformation and target recognition. Biochemistry. 2005;44:2173-82 pubmed
    ..modular pair (DocX), which comprises the C-terminal region of cellulosomal scaffoldin subunit from Clostridium thermocellum, to determine the effect of calcium on its structure and interaction with type II cohesin...
  59. Sparling R, Islam R, Cicek N, Carere C, Chow H, Levin D. Formate synthesis by Clostridium thermocellum during anaerobic fermentation. Can J Microbiol. 2006;52:681-8 pubmed
    We have detected formate synthesis by Clostridium thermocellum 27405 cultured in both cellobiose and alpha-cellulose. While formate synthesis has been reported for one strain of C. thermocellum (strain I-1-B), numerous studies of C...
  60. Islam R, Cicek N, Sparling R, Levin D. Effect of substrate loading on hydrogen production during anaerobic fermentation by Clostridium thermocellum 27405. Appl Microbiol Biotechnol. 2006;72:576-83 pubmed
    We have investigated hydrogen (H2) production by the cellulose-degrading anaerobic bacterium, Clostridium thermocellum. In the following experiments, batch-fermentations were carried out with cellobiose at three different substrate ..
  61. Mui Chan C, Zhou C, Brunzelle J, Huang R. Structural and biochemical insights into 2'-O-methylation at the 3'-terminal nucleotide of RNA by Hen1. Proc Natl Acad Sci U S A. 2009;106:17699-704 pubmed publisher
    ..Here, we report four crystal structures of the MTase domain of a bacterial homolog of Hen1 from Clostridium thermocellum and Anabaena variabilis, which are enzymatically indistinguishable from the eukaryotic Hen1 in their ..
  62. Maki M, Leung K, Qin W. The prospects of cellulase-producing bacteria for the bioconversion of lignocellulosic biomass. Int J Biol Sci. 2009;5:500-16 pubmed
    ..Furthermore, cellulosome-producing Clostridium thermocellum and its ability to ferment sugars to ethanol; its amenability to co-culture and, recent advances in ..
  63. Zverlov V, Kellermann J, Schwarz W. Functional subgenomics of Clostridium thermocellum cellulosomal genes: identification of the major catalytic components in the extracellular complex and detection of three new enzymes. Proteomics. 2005;5:3646-53 pubmed
    b>Clostridium thermocellum produces the most efficient enzyme-complex for the degradation of polysaccharides in biomass, the large extracellular cellulosome...
  64. Peng Y, Luo Y, Yu T, Xu X, Fan K, Zhao Y, et al. A blue native-PAGE analysis of membrane protein complexes in Clostridium thermocellum. BMC Microbiol. 2011;11:22 pubmed publisher
    b>Clostridium thermocellum is a Gram-positive thermophilic anaerobic bacterium with the unusual capacity to convert cellulosic biomass into ethanol and hydrogen. Identification and characterization of protein complexes in C...
  65. Wang P, Chan C, Christensen D, Zhang C, Selvadurai K, Huang R. Molecular basis of bacterial protein Hen1 activating the ligase activity of bacterial protein Pnkp for RNA repair. Proc Natl Acad Sci U S A. 2012;109:13248-53 pubmed publisher
    ..ligase consisting of the C-terminal half of Pnkp (Pnkp-C) and the N-terminal half of Hen1 (Hen1-N) from Clostridium thermocellum. The structure reveals that the N-terminal domain of Clostridium thermocellum (Cth) Hen1, shaped like a ..
  66. Mearls E, Izquierdo J, Lynd L. Formation and characterization of non-growth states in Clostridium thermocellum: spores and L-forms. BMC Microbiol. 2012;12:180 pubmed publisher
    b>Clostridium thermocellum is an anaerobic thermophilic bacterium that exhibits high levels of cellulose solublization and produces ethanol as an end product of its metabolism...
  67. Deng Y, Olson D, Zhou J, Herring C, Joe Shaw A, Lynd L. Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum. Metab Eng. 2013;15:151-8 pubmed publisher
    In Clostridium thermocellum, a thermophilic anaerobic bacterium able to rapidly ferment cellulose to ethanol, pyruvate kinase (EC 2.7.1.40) is absent based on both the genome sequence and enzymatic assays...
  68. Kitago Y, Karita S, Watanabe N, Kamiya M, Aizawa T, Sakka K, et al. Crystal structure of Cel44A, a glycoside hydrolase family 44 endoglucanase from Clostridium thermocellum. J Biol Chem. 2007;282:35703-11 pubmed
    The crystal structure of Cel44A, which is one of the enzymatic components of the cellulosome of Clostridium thermocellum, was solved at a resolution of 0.96 A. This enzyme belongs to glycoside hydrolase family (GH family) 44...
  69. Wang L, Smith P, Shuman S. Structure and mechanism of the 2',3' phosphatase component of the bacterial Pnkp-Hen1 RNA repair system. Nucleic Acids Res. 2013;41:5864-73 pubmed publisher
    ..Here we report the crystal structure of the phosphatase domain of Clostridium thermocellum Pnkp with Mn(2+) and citrate in the active site...
  70. Chinn M, Nokes S, Strobel H. Influence of process conditions on end product formation from Clostridium thermocellum 27405 in solid substrate cultivation on paper pulp sludge. Bioresour Technol. 2007;98:2184-93 pubmed
    ..duration of cultivation time (6, 10 and 14 days), on product formation (lactate, ethanol and acetate) by Clostridium thermocellum 27405 were examined during growth on paper pulp sludge...
  71. Rydzak T, Levin D, Cicek N, Sparling R. End-product induced metabolic shifts in Clostridium thermocellum ATCC 27405. Appl Microbiol Biotechnol. 2011;92:199-209 pubmed publisher
    ..of enzymes involved in pyruvate catabolism and fermentative end-product formation were studied in Clostridium thermocellum in response to the addition of individual end-products (H(2), acetate, ethanol, formate, and lactate) to ..
  72. Zhang Y, Lynd L. Biosynthesis of radiolabeled cellodextrins by the Clostridium thermocellum cellobiose and cellodextrin phosphorylases for measurement of intracellular sugars. Appl Microbiol Biotechnol. 2006;70:123-9 pubmed
    The Clostridium thermocellum cellobiose and cellodextrin phosphorylases (glucosyl transferases) in the cell extract were used to synthesize radiolabeled cellodextrins with a degree of polymerization (DP=2-6) from nonradioactive glucose-1-..
  73. Lilly M, Fierobe H, van Zyl W, Volschenk H. Heterologous expression of a Clostridium minicellulosome in Saccharomyces cerevisiae. FEMS Yeast Res. 2009;9:1236-49 pubmed publisher
    ..Far Western blot analysis confirmed that the Scaf3p is targeted to the yeast cell surface and that the Clostridium thermocellum cohesin domain is functional in yeast. Similarly, functionality of the C...
  74. Lv W, Yu Z. Isolation and characterization of two thermophilic cellulolytic strains of Clostridium thermocellum from a compost sample. J Appl Microbiol. 2013;114:1001-7 pubmed publisher
    ..To isolate, identify and characterize new thermophilic cellulolytic bacterial strains from a compost sample...
  75. Najmudin S, Pinheiro B, Prates J, Gilbert H, Romão M, Fontes C. Putting an N-terminal end to the Clostridium thermocellum xylanase Xyn10B story: crystal structure of the CBM22-1-GH10 modules complexed with xylohexaose. J Struct Biol. 2010;172:353-62 pubmed publisher
    ..Xyn10B from Clostridium thermocellum is a typical modular enzyme containing an N-terminal family 22 CBM (CBM22-1), a family 10 glycoside ..
  76. Nakai H, Hachem M, Petersen B, Westphal Y, Mannerstedt K, Baumann M, et al. Efficient chemoenzymatic oligosaccharide synthesis by reverse phosphorolysis using cellobiose phosphorylase and cellodextrin phosphorylase from Clostridium thermocellum. Biochimie. 2010;92:1818-26 pubmed publisher
    Inverting cellobiose phosphorylase (CtCBP) and cellodextrin phosphorylase (CtCDP) from Clostridium thermocellum ATCC27405 of glycoside hydrolase family 94 catalysed reverse phosphorolysis to produce cellobiose and cellodextrins in 57% and ..
  77. Argyros D, Tripathi S, Barrett T, Rogers S, Feinberg L, Olson D, et al. High ethanol titers from cellulose by using metabolically engineered thermophilic, anaerobic microbes. Appl Environ Microbiol. 2011;77:8288-94 pubmed publisher
    This work describes novel genetic tools for use in Clostridium thermocellum that allow creation of unmarked mutations while using a replicating plasmid. The strategy employed counter-selections developed from the native C...
  78. Demain A, Newcomb M, Wu J. Cellulase, clostridia, and ethanol. Microbiol Mol Biol Rev. 2005;69:124-54 pubmed
  79. Anbar M, Gul O, Lamed R, Sezerman U, Bayer E. Improved thermostability of Clostridium thermocellum endoglucanase Cel8A by using consensus-guided mutagenesis. Appl Environ Microbiol. 2012;78:3458-64 pubmed publisher
    ..2.1.4), Cel8A, from Clostridium thermocellum, using random error-prone PCR and a combination of three beneficial mutations, dominated by an intriguing ..
  80. Das S, Ravindran R, Ahmed S, Das D, Goyal D, Fontes C, et al. Bioethanol production involving recombinant C. thermocellum hydrolytic hemicellulase and fermentative microbes. Appl Biochem Biotechnol. 2012;167:1475-88 pubmed publisher
    ..coli cells harbouring the truncated 903 bp gene designated as glycoside hydrolase family 43 (GH43) from Clostridium thermocellum showing hemicellulase activity along with its further use in simultaneous saccharification and ..
  81. Shao X, Raman B, Zhu M, Mielenz J, Brown S, Guss A, et al. Mutant selection and phenotypic and genetic characterization of ethanol-tolerant strains of Clostridium thermocellum. Appl Microbiol Biotechnol. 2011;92:641-52 pubmed publisher
    b>Clostridium thermocellum is a model microorganism for converting cellulosic biomass into fuels and chemicals via consolidated bioprocessing...
  82. Roberts S, Gowen C, Brooks J, Fong S. Genome-scale metabolic analysis of Clostridium thermocellum for bioethanol production. BMC Syst Biol. 2010;4:31 pubmed publisher
    ..b>Clostridium thermocellum (ATCC 27405) is a thermophilic anaerobe that is both cellulolytic and ethanologenic, meaning that it can ..
  83. Correia M, Prates J, Brás J, Fontes C, Newman J, Lewis R, et al. Crystal structure of a cellulosomal family 3 carbohydrate esterase from Clostridium thermocellum provides insights into the mechanism of substrate recognition. J Mol Biol. 2008;379:64-72 pubmed publisher
    ..b>Clostridium thermocellum contains a single CE3 enzyme, CtCes3, which comprises two highly homologous (97% sequence identity) ..
  84. McQueen P, Spicer V, Rydzak T, Sparling R, Levin D, Wilkins J, et al. Information-dependent LC-MS/MS acquisition with exclusion lists potentially generated on-the-fly: case study using a whole cell digest of Clostridium thermocellum. Proteomics. 2012;12:1160-9 pubmed publisher
    ..vendors, we conducted a series of model experiments using a whole cell tryptic digestion of the bacterium Clostridium thermocellum. We ran a series of five iterative LC-MS/MS runs, adding a new exclusion list at each of four ..