clostridium kluyveri


Summary: A species of gram-positive bacteria in the family Clostridiaceae. It is distinctive for its ability to ferment ETHANOL to caproic acid.

Top Publications

  1. Li F, Hagemeier C, Seedorf H, Gottschalk G, Thauer R. Re-citrate synthase from Clostridium kluyveri is phylogenetically related to homocitrate synthase and isopropylmalate synthase rather than to Si-citrate synthase. J Bacteriol. 2007;189:4299-304 pubmed
    ..However, in Clostridium kluyveri and some other strictly anaerobic bacteria, the reaction is catalyzed by a Re-citrate synthase, whose ..
  2. Li F, Hinderberger J, Seedorf H, Zhang J, Buckel W, Thauer R. Coupled ferredoxin and crotonyl coenzyme A (CoA) reduction with NADH catalyzed by the butyryl-CoA dehydrogenase/Etf complex from Clostridium kluyveri. J Bacteriol. 2008;190:843-50 pubmed
    ..We report here that ferredoxin reduction with NADH in cell extracts from Clostridium kluyveri is catalyzed by the butyryl-CoA dehydrogenase/Etf complex and that the acetyl-CoA dependence previously ..
  3. Seedorf H, Fricke W, Veith B, Bruggemann H, Liesegang H, Strittmatter A, et al. The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features. Proc Natl Acad Sci U S A. 2008;105:2128-33 pubmed publisher
    b>Clostridium kluyveri is unique among the clostridia; it grows anaerobically on ethanol and acetate as sole energy sources. Fermentation products are butyrate, caproate, and H2. We report here the genome sequence of C...
  4. Chakraborty S, Sakka M, Kimura T, Sakka K. Cloning and expression of a Clostridium kluyveri gene responsible for diaphorase activity. Biosci Biotechnol Biochem. 2008;72:735-41 pubmed
    A small enzyme showing diaphorase activity was purified from culture supernatant of Clostridium kluyveri and its N-terminal amino acid sequence was determined...
  5. Heldt D, Frank S, Seyedarabi A, Ladikis D, Parsons J, Warren M, et al. Structure of a trimeric bacterial microcompartment shell protein, EtuB, associated with ethanol utilization in Clostridium kluyveri. Biochem J. 2009;423:199-207 pubmed publisher
    It has been suggested that ethanol metabolism in the strict anaerobe Clostridium kluyveri occurs within a metabolosome, a subcellular proteinaceous bacterial microcompartment...
  6. Li Z, Shi Z, Jian J, Guo Y, Wu Q, Chen G. Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) from unrelated carbon sources by metabolically engineered Escherichia coli. Metab Eng. 2010;12:352-9 pubmed publisher
    ..Genes involved in succinate degradation in Clostridium kluyveri and P(3HB) accumulation pathway of Ralstonia eutropha were co-expressed for the synthesis of the above ..
  7. Wang S, Huang H, Moll J, Thauer R. NADP+ reduction with reduced ferredoxin and NADP+ reduction with NADH are coupled via an electron-bifurcating enzyme complex in Clostridium kluyveri. J Bacteriol. 2010;192:5115-23 pubmed publisher
    It was recently found that the cytoplasmic butyryl-coenzyme A (butyryl-CoA) dehydrogenase-EtfAB complex from Clostridium kluyveri couples the exergonic reduction of crotonyl-CoA to butyryl-CoA with NADH and the endergonic reduction of ..
  8. Weimer P, Stevenson D. Isolation, characterization, and quantification of Clostridium kluyveri from the bovine rumen. Appl Microbiol Biotechnol. 2012;94:461-6 pubmed publisher
    A strain of Clostridium kluyveri was isolated from the bovine rumen in a medium containing ethanol as an electron donor and acetate and succinate (common products of rumen fermentation) as electron acceptors...
  9. Hu X, Du H, Ren C, Xu Y. Illuminating Anaerobic Microbial Community and Cooccurrence Patterns across a Quality Gradient in Chinese Liquor Fermentation Pit Muds. Appl Environ Microbiol. 2016;82:2506-15 pubmed publisher
    ..Species richness, biodiversity, and relative and/or absolute abundances of Clostridia,Clostridium kluyveri, Bacteroidia, and Methanobacteria significantly increased, with corresponding increases in levels of pH, ..

More Information


  1. Kim M, Le H, Xie X, Feng X, Tang Y, Mouttaki H, et al. Two pathways for glutamate biosynthesis in the syntrophic bacterium Syntrophus aciditrophicus. Appl Environ Microbiol. 2015;81:8434-44 pubmed publisher
    ..Besides Clostridium kluyveri, this study reveals the second example of a microbial species to employ two pathways for glutamate ..
  2. Zhang Q, Yuan Y, Liao Z, Zhang W. Use of microbial indicators combined with environmental factors coupled with metrology tools for discrimination and classification of Luzhou-flavoured pit muds. J Appl Microbiol. 2017;123:933-943 pubmed publisher
    ..Actinobacteria, Lactobacillus, Bacillus, Methanosarcina, Methanocorpusculum, Methanoculleus and Clostridium kluyveri were microbial indicators in Luzhou-flavoured pit muds. They were detected by real-time quantitative PCR...
  3. Hu X, Du H, Xu Y. Identification and quantification of the caproic acid-producing bacterium Clostridium kluyveri in the fermentation of pit mud used for Chinese strong-aroma type liquor production. Int J Food Microbiol. 2015;214:116-22 pubmed publisher
    ..Among them, Clostridium kluyveri was identified as the main producer of caproic acid. One representative strain C...
  4. Lonkar S, Fu Z, Holtzapple M. Optimum alcohol concentration for chain elongation in mixed-culture fermentation of cellulosic substrate. Biotechnol Bioeng. 2016;113:2597-2604 pubmed publisher
    ..Microorganisms such as Clostridium kluyveri elongate short-chain acids by combining them with alcohol...
  5. Thauer R. My Lifelong Passion for Biochemistry and Anaerobic Microorganisms. Annu Rev Microbiol. 2015;69:1-30 pubmed publisher
    ..The scientific puzzle that fascinated me most was the metabolism of the anaerobic bacterium Clostridium kluyveri, which I took on in 1965 in Karl Decker's lab in Freiburg, Germany...
  6. Yoshida Y, Sato M, Kezuka Y, Hasegawa Y, Nagano K, Takebe J, et al. Acyl-CoA reductase PGN_0723 utilizes succinyl-CoA to generate succinate semialdehyde in a butyrate-producing pathway of Porphyromonas gingivalis. Arch Biochem Biophys. 2016;596:138-48 pubmed publisher
    ..6% identical with CoA-dependent succinate semialdehyde dehydrogenase (SSADH) in Clostridium kluyveri. By contrast, the protein has limited identity (19.1%) with CoA-independent SSADH in Escherichia coli...
  7. Leng L, Yang P, Mao Y, Wu Z, Zhang T, Lee P. Thermodynamic and physiological study of caproate and 1,3-propanediol co-production through glycerol fermentation and fatty acids chain elongation. Water Res. 2017;114:200-209 pubmed publisher
    ..A synergistic relationship was evinced through microbial characterization, resulting in Clostridium kluyveri and some bacteria with function of converting glycerol to VFAs...
  8. Reddy M, Mohan S, Chang Y. Medium-Chain Fatty Acids (MCFA) Production Through Anaerobic Fermentation Using Clostridium kluyveri: Effect of Ethanol and Acetate. Appl Biochem Biotechnol. 2018;185:594-605 pubmed publisher
    ..In the present study, MCFA production was evaluated with acetate and ethanol using the bacteria Clostridium kluyveri. Effects of substrate, electron donor, and methane inhibitor on MCFA production were evaluated...
  9. Chen X, Yin J, Ye J, Zhang H, Che X, Ma Y, et al. Engineering Halomonas bluephagenesis TD01 for non-sterile production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate). Bioresour Technol. 2017;244:534-541 pubmed publisher
    ..Gene orfZ encoding 4HB-CoA transferase of Clostridium kluyveri was integrated into the genome to achieve P(3HB-co-4HB) accumulation comparable to that of strains encoding ..
  10. Weimer P, Kohn R. Impacts of ruminal microorganisms on the production of fuels: how can we intercede from the outside?. Appl Microbiol Biotechnol. 2016;100:3389-98 pubmed publisher
    ..g., Clostridium kluyveri or Megasphaera elsdenii) that facilitate production of valeric and caproic acids...
  11. Hu L, Huang H, Yuan H, Tao F, Xie H, Wang S. Rex in Clostridium kluyveri is a global redox-sensing transcriptional regulator. J Biotechnol. 2016;233:17-25 pubmed publisher
    b>Clostridium kluyveri is unique in fermenting ethanol and acetate to butyrate, caproate, and H2...
  12. Weimer P, Nerdahl M, Brandl D. Production of medium-chain volatile fatty acids by mixed ruminal microorganisms is enhanced by ethanol in co-culture with Clostridium kluyveri. Bioresour Technol. 2015;175:97-101 pubmed publisher
    ..Co-culture of mixed ruminal microbes with a rumen-derived strain of the bacterium Clostridium kluyveri converted cellulosic biomass (alfalfa stems or switchgrass herbage) plus ethanol to VFA mixtures that ..
  13. Yin Y, Zhang Y, Karakashev D, Wang J, Angelidaki I. Biological caproate production by Clostridium kluyveri from ethanol and acetate as carbon sources. Bioresour Technol. 2017;241:638-644 pubmed publisher
    ..42g/L was achieved from high ethanol strength wastewater with an ethanol/acetate ratio of 10:1 (550mM total carbon). Results obtained in this study can pave the way towards efficient chain elongation from ethanol-rich wastewater. ..
  14. Chakraborty S, Sakka M, Kimura T, Sakka K. Characterization of a dihydrolipoyl dehydrogenase having diaphorase activity of Clostridium kluyveri. Biosci Biotechnol Biochem. 2008;72:982-8 pubmed
    The Clostridium kluyveri bfmBC gene encoding a putative dihydrolipoyl dehydrogenase (DLD; EC was expressed in Escherichia coli, and the recombinant enzyme rBfmBC was characterized...
  15. Zhou X, Yuan X, Shi Z, Meng D, Jiang W, Wu L, et al. Hyperproduction of poly(4-hydroxybutyrate) from glucose by recombinant Escherichia coli. Microb Cell Fact. 2012;11:54 pubmed publisher
    ..At present, high production cost is a big obstacle for large scale production of poly(4HB)...
  16. Bhushan B, Halasz A, Hawari J. Stereo-specificity for pro-(R) hydrogen of NAD(P)H during enzyme-catalyzed hydride transfer to CL-20. Biochem Biophys Res Commun. 2005;337:1080-3 pubmed
    A dehydrogenase from Clostridium sp. EDB2 and a diaphorase from Clostridium kluyveri were reacted with CL-20 to gain insights into the enzyme-catalyzed hydride transfer to CL-20, and the enzyme's stereo-specificity for either pro-R or pro-..
  17. Stibingerová A, Velvarská H, Kynclová K, Marounková B, Spundová M, Entlicher G. Lipoamide dehydrogenase and diaphorase catalyzed conversion of some NO donors to NO and reduction of NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO). Gen Physiol Biophys. 2009;28:384-90 pubmed
    ..In summary, we found that LAD significantly accelerates the conversion of a defined subset of NO donors to NO, especially GTN, and eliminates the NO scavenging effect of PTIO...
  18. Davis M, Shen M, Simeonov A, Hall M. Diaphorase Coupling Protocols for Red-Shifting Dehydrogenase Assays. Assay Drug Dev Technol. 2016;14:207-12 pubmed publisher
    ..This coupling technique is applicable to dehydrogenases that either produce or consume NAD(P)H, and the examples provided here can act as guidelines for the development of high-throughput screens against this enzyme class. ..
  19. Koch C, Kuchenbuch A, Kracke F, Bernhardt P, Krömer J, Harnisch F. Predicting and experimental evaluating bio-electrochemical synthesis - A case study with Clostridium kluyveri. Bioelectrochemistry. 2017;118:114-122 pubmed publisher
    ..challenges for the bio-electrochemical fermentation (electro-fermentation) of mid-chain organic acids using Clostridium kluyveri. Starting from a metabolic model the potential limitations of the metabolism as well as beneficial ..