clostridium cellulovorans

Summary

Summary: A species of gram-positive, cellulolytic bacteria in the family Clostridiaceae. It produces CELLULOSOMES which are involved in plant CELL WALL degradation.

Top Publications

  1. Jeng W, Wang N, Lin M, Lin C, Liaw Y, Chang W, et al. Structural and functional analysis of three ?-glucosidases from bacterium Clostridium cellulovorans, fungus Trichoderma reesei and termite Neotermes koshunensis. J Struct Biol. 2011;173:46-56 pubmed publisher
    ..mg/l) in Escherichia coli of the three functional ?-glucosidase genes was obtained from the bacterium Clostridium cellulovorans (CcBglA), the fungus Trichoderma reesei (TrBgl2), and the termite Neotermes koshunensis (NkBgl) with the ..
  2. Jeon S, Yu K, Kim S, Han S. A celluloytic complex from Clostridium cellulovorans consisting of mannanase B and endoglucanase E has synergistic effects on galactomannan degradation. Appl Microbiol Biotechnol. 2011;90:565-72 pubmed publisher
    ..and identified a putative cellulosomal mannanase belonging to the glycosyl hydrolase family 26 from Clostridium cellulovorans in xylan-containing cultures. In this study, a mannanase gene, manB from C...
  3. Koukiekolo R, Cho H, Kosugi A, Inui M, Yukawa H, Doi R. Degradation of corn fiber by Clostridium cellulovorans cellulases and hemicellulases and contribution of scaffolding protein CbpA. Appl Environ Microbiol. 2005;71:3504-11 pubmed
    b>Clostridium cellulovorans, an anaerobic bacterium, degrades native substrates efficiently by producing an extracellular enzyme complex called the cellulosome...
  4. Ciolacu D, Kovac J, Kokol V. The effect of the cellulose-binding domain from Clostridium cellulovorans on the supramolecular structure of cellulose fibers. Carbohydr Res. 2010;345:621-30 pubmed publisher
    ..The effect of the CBD from Clostridium cellulovorans on the supramolecular structure of three different sources of cellulose (cotton cellulose, spruce ..
  5. Bianchetti C, BRUMM P, Smith R, Dyer K, Hura G, Rutkoski T, et al. Structure, dynamics, and specificity of endoglucanase D from Clostridium cellulovorans. J Mol Biol. 2013;425:4267-85 pubmed publisher
    ..Endoglucanase D (EngD) from Clostridium cellulovorans is a modular enzyme comprising an N-terminal catalytic domain and a C-terminal carbohydrate-binding ..
  6. Cha J, Matsuoka S, Chan H, Yukawa H, Inui M, Doi R. Effect of multiple copies of cohesins on cellulase and hemicellulase activities of Clostridium cellulovorans mini-cellulosomes. J Microbiol Biotechnol. 2007;17:1782-8 pubmed
    Cellulosomes in Clostridium cellulovorans are assembled by the interaction between the repeated cohesin domains of a scaffolding protein (CbpA) and the dockerin domain of enzyme components...
  7. Meguro H, Morisaka H, Kuroda K, Miyake H, Tamaru Y, Ueda M. Putative role of cellulosomal protease inhibitors in Clostridium cellulovorans based on gene expression and measurement of activities. J Bacteriol. 2011;193:5527-30 pubmed publisher
    ..to demonstrate the activity of putative cellulosomal protease/peptidase inhibitors (named cyspins) of Clostridium cellulovorans, using the Saccharomyces cerevisiae display system...
  8. Tamaru Y, Miyake H, Kuroda K, Nakanishi A, Matsushima C, Doi R, et al. Comparison of the mesophilic cellulosome-producing Clostridium cellulovorans genome with other cellulosome-related clostridial genomes. Microb Biotechnol. 2011;4:64-73 pubmed publisher
    b>Clostridium cellulovorans, an anaerobic and mesophilic bacterium, degrades native substrates in soft biomass such as corn fibre and rice straw efficiently by producing an extracellular enzyme complex called the cellulosome...
  9. Hyeon J, Yu K, Suh D, Suh Y, Lee S, Lee J, et al. Production of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae. FEMS Microbiol Lett. 2010;310:39-47 pubmed publisher
    ..for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum...

More Information

Publications50

  1. Tamaru Y, Miyake H, Kuroda K, Nakanishi A, Kawade Y, Yamamoto K, et al. Genome sequence of the cellulosome-producing mesophilic organism Clostridium cellulovorans 743B. J Bacteriol. 2010;192:901-2 pubmed publisher
    b>Clostridium cellulovorans 743B was isolated from a wood chip pile and is an anaerobic and mesophilic spore-forming bacterium...
  2. Matsuoka S, Yukawa H, Inui M, Doi R. Synergistic interaction of Clostridium cellulovorans cellulosomal cellulases and HbpA. J Bacteriol. 2007;189:7190-4 pubmed
    b>Clostridium cellulovorans, an anaerobic bacterium, produces a small nonenzymatic protein called HbpA, which has a surface layer homology domain and a type I cohesin domain similar to those found in the cellulosomal scaffolding protein ..
  3. Han S, Yukawa H, Inui M, Doi R. Isolation and expression of the xynB gene and its product, XynB, a consistent component of the Clostridium cellulovorans cellulosome. J Bacteriol. 2004;186:8347-55 pubmed
    The nucleotide sequence of the Clostridium cellulovorans xynB gene, which encodes the XynB xylanase, consists of 1,821 bp and encodes a protein of 607 amino acids with a molecular weight of 65,976...
  4. Arai T, Matsuoka S, Cho H, Yukawa H, Inui M, Wong S, et al. Synthesis of Clostridium cellulovorans minicellulosomes by intercellular complementation. Proc Natl Acad Sci U S A. 2007;104:1456-60 pubmed
    ..Three strains of Bacillus subtilis were constructed to express Clostridium cellulovorans genes engB, xynB, and minicbpA...
  5. Kosugi A, Arai T, Doi R. Degradation of cellulosome-produced cello-oligosaccharides by an extracellular non-cellulosomal beta-glucan glucohydrolase, BglA, from Clostridium cellulovorans. Biochem Biophys Res Commun. 2006;349:20-3 pubmed
    b>Clostridium cellulovorans degrades cellulose efficiently to small oligosaccharides, which are used as an energy source...
  6. Arai T, Kosugi A, Chan H, Koukiekolo R, Yukawa H, Inui M, et al. Properties of cellulosomal family 9 cellulases from Clostridium cellulovorans. Appl Microbiol Biotechnol. 2006;71:654-60 pubmed
    The cellulosomal family 9 cellulase genes engH, engK, engL, engM, and engY of Clostridium cellulovorans have been cloned and sequenced. We compared the enzyme activity of family 9 cellulosomal cellulases from C...
  7. Han S, Yukawa H, Inui M, Doi R. Effect of carbon source on the cellulosomal subpopulations of Clostridium cellulovorans. Microbiology. 2005;151:1491-7 pubmed
    b>Clostridium cellulovorans produces a cellulase enzyme complex called the cellulosome...
  8. Tamaru Y, Miyake H, Kuroda K, Ueda M, Doi R. Comparative genomics of the mesophilic cellulosome-producing Clostridium cellulovorans and its application to biofuel production via consolidated bioprocessing. Environ Technol. 2010;31:889-903 pubmed publisher
    b>Clostridium cellulovorans is an anaerobic, mesophilic bacterium that efficiently degrades native substrates in soft biomass such as corn fibre and rice straw by producing an extracellular enzyme complex called the cellulosomes...
  9. Ortiz de Ora L, Muñoz Gutiérrez I, Bayer E, Shoham Y, Lamed R, Borovok I. Revisiting the Regulation of the Primary Scaffoldin Gene in Clostridium thermocellum. Appl Environ Microbiol. 2017;83: pubmed publisher
    ..strain Bc-iso-3. Interestingly, a previously identified TSS of the primary scaffoldin CbpA gene of Clostridium cellulovorans matches the predicted σI-dependent promoter identified in the present work rather than ..
  10. Inamori T, Aburaya S, Morisaka H, Kuroda K, Ueda M. Characteristic strategy of assimilation of various saccharides by Clostridium cellulovorans. AMB Express. 2016;6:64 pubmed publisher
    b>Clostridium cellulovorans can effectively assimilate not only cellulose but also hemicellulose by producing cellulosomal and non-cellulosomal enzymes. However, little is known about how C...
  11. Wen Z, Minton N, Zhang Y, Li Q, Liu J, Jiang Y, et al. Enhanced solvent production by metabolic engineering of a twin-clostridial consortium. Metab Eng. 2017;39:38-48 pubmed publisher
    ..we developed a consolidated bioprocessing (CBP) based on a twin-clostridial consortium composed of Clostridium cellulovorans and Clostridium beijerinckii capable of producing cellulosic butanol from alkali-extracted, deshelled ..
  12. Flannelly D, Aoki T, Aristilde L. Short-time dynamics of pH-dependent conformation and substrate binding in the active site of beta-glucosidases: A computational study. J Struct Biol. 2015;191:352-64 pubmed publisher
    ..to explore conformational and substrate binding dynamics in two well-characterized BGs of bacterial (Clostridium cellulovorans) and fungal (Trichoderma reesei) origins as a function of pH...
  13. Shin S, Hyeon J, Kim Y, Kang D, Kim S, Park C, et al. Enhanced hydrolysis of lignocellulosic biomass: Bi-functional enzyme complexes expressed in Pichia pastoris improve bioethanol production from Miscanthus sinensis. Biotechnol J. 2015;10:1912-9 pubmed publisher
    ..Xylanase XynB from Clostridium cellulovorans and a chimeric endoglucanase cCelE from Clostridium thermocellum were selected as enzyme subunits, and ..
  14. Lu H, Chen J, Jia Y, Cai M, Lee P. Transcriptomic Responses of the Interactions between Clostridium cellulovorans 743B and Rhodopseudomonas palustris CGA009 in a Cellulose-Grown Coculture for Enhanced Hydrogen Production. Appl Environ Microbiol. 2016;82:4546-4559 pubmed publisher
    ..sequencing was used to query the global transcriptomic responses of an artificial coculture of Clostridium cellulovorans 743B and Rhodopseudomonas palustris CGA009...
  15. Yamamoto K, Tamaru Y. Synergistic properties of cellulases from Clostridium cellulovorans in the presence of cellobiose. AMB Express. 2016;6:1 pubmed publisher
    An anaerobic mesophile, Clostridium cellulovorans, produces a multienzyme complex called the cellulosome and actively degrades polysaccharides in the plant cell wall. C...
  16. Yang X, Xu M, Yang S. Metabolic and process engineering of Clostridium cellulovorans for biofuel production from cellulose. Metab Eng. 2015;32:39-48 pubmed publisher
    ..Here we engineered a cellulolytic bacterium, Clostridium cellulovorans, for n-butanol and ethanol production directly from cellulose by introducing an aldehyde/alcohol ..
  17. Miyake H, Maeda Y, Ishikawa T, Tanaka A. Calorimetric studies of the growth of anaerobic microbes. J Biosci Bioeng. 2016;122:364-9 pubmed publisher
    ..Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium cellulovorans were used as representative anaerobic microbes...
  18. Li P, Zhang C, Xu D. Molecular dynamics investigations of cello-oligosaccharide recognition by Cel9G-CBM3c from Clostridium cellulovorans. Phys Chem Chem Phys. 2018;20:5235-5245 pubmed publisher
    ..the specificity of the recognition of the polysaccharide by the multidomain endoglucanase Cel9G from Clostridium cellulovorans was investigated by molecular dynamics simulations...
  19. Nakajima D, Shibata T, Tanaka R, Kuroda K, Ueda M, Miyake H. Characterization of the cellulosomal scaffolding protein CbpC from Clostridium cellulovorans 743B. J Biosci Bioeng. 2017;124:376-380 pubmed publisher
    b>Clostridium cellulovorans 743B, an anaerobic and mesophilic bacterium, produces an extracellular enzyme complex called the cellulosome on the cell surface. Recently, we have reported the whole genome sequence of C...
  20. Kim S, Kim S, Shin S, Hyeon J, Han S. Mutation of a conserved tryptophan residue in the CBM3c of a GH9 endoglucanase inhibits activity. Int J Biol Macromol. 2016;92:159-166 pubmed publisher
    ..module (CBM3c) is important for the catalytic activity of family 9 endoglucanases such as the EngZ from Clostridium cellulovorans. To determine the role of CBM3c in catalytic activity, we made a tryptophan to alanine substitution ..
  21. Boraston A. The interaction of carbohydrate-binding modules with insoluble non-crystalline cellulose is enthalpically driven. Biochem J. 2005;385:479-84 pubmed
    ..that this is not the case for the high-affinity interactions of CcCBM17 (the family 17 CBM from EngF of Clostridium cellulovorans) and BspCBM28 (the family 28 CBM from Cel5A of Bacillus species 1139) with regenerated cellulose, an ..
  22. Jeon E, Hyeon J, Eun L, Park B, Kim S, Lee J, et al. Cellulosic alcoholic fermentation using recombinant Saccharomyces cerevisiae engineered for the production of Clostridium cellulovorans endoglucanase and Saccharomycopsis fibuligera beta-glucosidase. FEMS Microbiol Lett. 2009;301:130-6 pubmed publisher
    ..saccharification and fermentation of cellulose by the overexpression of the endoglucanase D (EngD) from Clostridium cellulovorans and the beta-glucosidase (Bgl1) from Saccharomycopsis fibuligera...
  23. Jeon S, Lee J, Kim S, Kim S, Han S. Analysis of selective, high protein-protein binding interaction of cohesin-dockerin complex using biosensing methods. Biosens Bioelectron. 2012;35:382-9 pubmed publisher
    ..results displayed diverse binding profiles to the dockerin containing cellulosomal proteins produced by Clostridium cellulovorans grown on different carbon sources, such as Avicel, xylan and AXP (Avicel:xylan:pectin (3:1:1))...
  24. Song E, Ryu K. A pepper mottle virus-based vector enables systemic expression of endoglucanase D in non-transgenic plants. Arch Virol. 2017;162:3717-3726 pubmed publisher
    ..Using a virus-based vector, we investigated the feasibility of producing the endoglucanase D (EngD) from Clostridium cellulovorans in Nicotiana benthamiana...
  25. Yeh M, Craig S, Lum M, Foong F. Effects of the PT region of EngD and HLD of CbpA on solubility, catalytic activity and purification characteristics of EngD-CBD(CbpA) fusions from Clostridium cellulovorans. J Biotechnol. 2005;116:233-44 pubmed
    ..hydrophilic domain (HLD) and cellulose binding domain (CBD) of cellulose binding protein A (CbpA) from Clostridium cellulovorans were constructed, expressed, and analyzed...
  26. Cho W, Jeon S, Shim H, Doi R, Han S. Cellulosomic profiling produced by Clostridium cellulovorans during growth on different carbon sources explored by the cohesin marker. J Biotechnol. 2010;145:233-9 pubmed publisher
    b>Clostridium cellulovorans produces large extracellular enzyme complex, called cellulosomes. The diversity of the cellulosomal enzymes, which are secreted by C...
  27. Zhao L, Pang Q, Xie J, Pei J, Wang F, Fan S. Enzymatic properties of Thermoanaerobacterium thermosaccharolyticum ?-glucosidase fused to Clostridium cellulovorans cellulose binding domain and its application in hydrolysis of microcrystalline cellulose. BMC Biotechnol. 2013;13:101 pubmed publisher
    ..This will increase the cost of cellulose degradation...
  28. Jeon S, Lee J, Kim S, Park S, Choi G, Han S. Unique contribution of the cell wall-binding endoglucanase G to the cellulolytic complex in Clostridium cellulovorans. Appl Environ Microbiol. 2013;79:5942-8 pubmed publisher
    The cellulosomes produced by Clostridium cellulovorans are organized by the specific interactions between the cohesins in the scaffolding proteins and the dockerins of the catalytic components...
  29. Jeon S, Yu K, Kim S, Han S. The processive endoglucanase EngZ is active in crystalline cellulose degradation as a cellulosomal subunit of Clostridium cellulovorans. N Biotechnol. 2012;29:365-71 pubmed publisher
    b>Clostridium cellulovorans produces an efficient enzyme complex for the degradation of lignocellulosic biomass...
  30. Lee H, Chang C, Teng K, Liang P. Construction and characterization of different fusion proteins between cellulases and ?-glucosidase to improve glucose production and thermostability. Bioresour Technol. 2011;102:3973-6 pubmed publisher
    A ?-glucosidase from Clostridium cellulovorans (CcBG) was fused with one of three different types of cellulases from Clostridium thermocellum, including a cellulosomal endoglucanase CelD (CtCD), a cellulosomal exoglucanase CBHA (CtCA) ..
  31. Yang X, Xu M, Yang S. Restriction modification system analysis and development of in vivo methylation for the transformation of Clostridium cellulovorans. Appl Microbiol Biotechnol. 2016;100:2289-99 pubmed publisher
    b>Clostridium cellulovorans, a cellulolytic bacterium producing butyric and acetic acids as main fermentation products, is a promising host for biofuel production from cellulose. However, the transformation method of C...
  32. Matsui K, Bae J, Esaka K, Morisaka H, Kuroda K, Ueda M. Exoproteome profiles of Clostridium cellulovorans grown on various carbon sources. Appl Environ Microbiol. 2013;79:6576-84 pubmed publisher
    ..This complex is considered the most efficient system for cellulose degradation. Clostridium cellulovorans, which is known to produce cellulosomes, changes the composition of its cellulosomes depending on the ..
  33. Lavan L, Van Dyk J, Chan H, Doi R, Pletschke B. Effect of physical conditions and chemicals on the binding of a mini-CbpA from Clostridium cellulovorans to a semi-crystalline cellulose ligand. Lett Appl Microbiol. 2009;48:419-25 pubmed publisher
    To investigate the effect that environmental factors have on Clostridium cellulovorans cellulose binding domain (CBD) binding to a semi-crystalline cellulose ligand, namely Avicel.
  34. Nahalka J. Physiological aggregation of maltodextrin phosphorylase from Pyrococcus furiosus and its application in a process of batch starch degradation to alpha-D-glucose-1-phosphate. J Ind Microbiol Biotechnol. 2008;35:219-23 pubmed
    ..phosphorylase from Pyrococcus furiosus (PF1535) was fused with the cellulose-binding domain of Clostridium cellulovorans serving as an aggregation module...
  35. Ota M, Sakuragi H, Morisaka H, Kuroda K, Miyake H, Tamaru Y, et al. Display of Clostridium cellulovorans xylose isomerase on the cell surface of Saccharomyces cerevisiae and its direct application to xylose fermentation. Biotechnol Prog. 2013;29:346-51 pubmed publisher
    ..Genomic analysis of the bacterium Clostridium cellulovorans revealed the presence of XI-related genes. In this study, XI derived from C...
  36. Hyeon J, Jeon W, Whang S, Han S. Production of minicellulosomes for the enhanced hydrolysis of cellulosic substrates by recombinant Corynebacterium glutamicum. Enzyme Microb Technol. 2011;48:371-7 pubmed publisher
    ..strain expressing functional minicellulosomes containing chimeric endoglucanase E bound to miniCbpA from Clostridium cellulovorans that can hydrolyze cellulosic materials...
  37. Bayer E, Belaich J, Shoham Y, Lamed R. The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides. Annu Rev Microbiol. 2004;58:521-54 pubmed
  38. Lee C, Yu K, Kim S, Han S. Enhancement of the thermostability and activity of mesophilic Clostridium cellulovorans EngD by in vitro DNA recombination with Clostridium thermocellum CelE. J Biosci Bioeng. 2010;109:331-6 pubmed publisher
    The thermal stability and catalytic activity of endoglucanase (EngD) from mesophilic Clostridium cellulovorans were improved by evolutionary molecular engineering...
  39. Kim S, Joo J, Jeon S, Hyeon J, Kim S, Um Y, et al. Enhanced thermostability of mesophilic endoglucanase Z with a high catalytic activity at active temperatures. Int J Biol Macromol. 2016;86:269-76 pubmed publisher
    This is the first study for therrmostable mutants of mesophilic endoglucanase EngZ from Clostridium cellulovorans using by site-directed mutagenesis...
  40. Xu Y, Foong F. Characterization of a cellulose binding domain from Clostridium cellulovorans endoglucanase-xylanase D and its use as a fusion partner for soluble protein expression in Escherichia coli. J Biotechnol. 2008;135:319-25 pubmed publisher
    Different chimeric proteins combining the non-catalytic C-terminal putative cellulose binding domain of Clostridium cellulovorans endoglucanase-xylanase D (EngD) with its proline-threonine rich region PT-linker, PTCBD(EngD), cellulose ..
  41. Murashima K, Kosugi A, Doi R. Site-directed mutagenesis and expression of the soluble form of the family IIIa cellulose binding domain from the cellulosomal scaffolding protein of Clostridium cellulovorans. J Bacteriol. 2005;187:7146-9 pubmed
    ..residues of the family IIIa cellulose binding domain (CBD) from the cellulosomal scaffolding protein of Clostridium cellulovorans were investigated by site-directed mutagenesis and cellulose binding studies...