clostridium acetobutylicum

Summary

Summary: A species of gram-positive bacteria in the family Clostridiaceae, used for the industrial production of SOLVENTS.

Top Publications

  1. Dürre P. Biobutanol: an attractive biofuel. Biotechnol J. 2007;2:1525-34 pubmed
    ..The best-studied bacterium to perform a butanol fermentation is Clostridium acetobutylicum. Its genome has been sequenced, and the regulation of solvent formation is under intensive investigation...
  2. Sullivan L, Bennett G. Proteome analysis and comparison of Clostridium acetobutylicum ATCC 824 and Spo0A strain variants. J Ind Microbiol Biotechnol. 2006;33:298-308 pubmed
    The proteomic profiles of several Clostridium acetobutylicum strains were compared by two-dimensional gel electrophoresis and mass spectroscopy. The proteomic profile of C...
  3. Lee S, Park J, Jang S, Nielsen L, Kim J, Jung K. Fermentative butanol production by Clostridia. Biotechnol Bioeng. 2008;101:209-28 pubmed publisher
    ..The strategies for strain improvement by metabolic engineering and further requirements to make fermentative butanol production a successful industrial process are also discussed...
  4. Millat T, Janssen H, Thorn G, King J, Bahl H, Fischer R, et al. A shift in the dominant phenotype governs the pH-induced metabolic switch of Clostridium acetobutylicumin phosphate-limited continuous cultures. Appl Microbiol Biotechnol. 2013;97:6451-66 pubmed publisher
    In response to changing extracellular pH levels, phosphate-limited continuous cultures of Clostridium acetobutylicum reversibly switches its metabolism from the dominant formation of acids to the prevalent production of solvents...
  5. Lehmann D, Lütke Eversloh T. Switching Clostridium acetobutylicum to an ethanol producer by disruption of the butyrate/butanol fermentative pathway. Metab Eng. 2011;13:464-73 pubmed publisher
    ..genetic engineering tools, a targeted 3-hydroxybutyryl-CoA dehydrogenase (Hbd)-negative mutant of Clostridium acetobutylicum was generated. Interestingly, the entire butyrate/butanol (C(4)) metabolic pathway of C...
  6. Fischer R, Oehmcke S, Meyer U, Mix M, Schwarz K, Fiedler T, et al. Transcription of the pst operon of Clostridium acetobutylicum is dependent on phosphate concentration and pH. J Bacteriol. 2006;188:5469-78 pubmed
    The pst operon of Clostridium acetobutylicum ATCC 824 comprises five genes, pstS, pstC, pstA, pstB, and phoU, and shows a gene architecture identical to that of Escherichia coli...
  7. Tracy B, Jones S, Papoutsakis E. Inactivation of ?E and ?G in Clostridium acetobutylicum illuminates their roles in clostridial-cell-form biogenesis, granulose synthesis, solventogenesis, and spore morphogenesis. J Bacteriol. 2011;193:1414-26 pubmed publisher
    ..an initial analysis to elucidate their roles in sporulation-related morphogenesis and solventogenesis in Clostridium acetobutylicum. The terminal differentiation phenotype for the sigE inactivation mutant stalled in sporulation prior to ..
  8. Scotcher M, Rudolph F, Bennett G. Expression of abrB310 and SinR, and effects of decreased abrB310 expression on the transition from acidogenesis to solventogenesis, in Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol. 2005;71:1987-95 pubmed
    ..to sinR and three highly similar homologues to abrB, designated abrB310, abrB1941, and abrB3647, in Clostridium acetobutylicum ATCC 824...
  9. Gonzalez Pajuelo M, Meynial Salles I, Mendes F, Soucaille P, Vasconcelos I. Microbial conversion of glycerol to 1,3-propanediol: physiological comparison of a natural producer, Clostridium butyricum VPI 3266, and an engineered strain, Clostridium acetobutylicum DG1(pSPD5). Appl Environ Microbiol. 2006;72:96-101 pubmed
    b>Clostridium acetobutylicum is not able to grow on glycerol as the sole carbon source since it cannot reoxidize the excess of NADH generated by glycerol catabolism...

More Information

Publications87

  1. Yu Y, Tangney M, Aass H, Mitchell W. Analysis of the mechanism and regulation of lactose transport and metabolism in Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol. 2007;73:1842-50 pubmed
    Although the acetone-butanol-ethanol fermentation of Clostridium acetobutylicum is currently uneconomic, the ability of the bacterium to metabolize a wide range of carbohydrates offers the potential for revival based on the use of cheap, ..
  2. Wang S, Zhang Y, Dong H, Mao S, Zhu Y, Wang R, et al. Formic acid triggers the "Acid Crash" of acetone-butanol-ethanol fermentation by Clostridium acetobutylicum. Appl Environ Microbiol. 2011;77:1674-80 pubmed publisher
    Solvent production by Clostridium acetobutylicum collapses when cells are grown in pH-uncontrolled glucose medium, the so-called "acid crash" phenomenon...
  3. Amador Noguez D, Brasg I, Feng X, Roquet N, Rabinowitz J. Metabolome remodeling during the acidogenic-solventogenic transition in Clostridium acetobutylicum. Appl Environ Microbiol. 2011;77:7984-97 pubmed publisher
    The fermentation carried out by the biofuel producer Clostridium acetobutylicum is characterized by two distinct phases. Acidogenesis occurs during exponential growth and involves the rapid production of acids (acetate and butyrate)...
  4. Hou S, Jones S, Choe L, Papoutsakis E, Lee K. Workflow for quantitative proteomic analysis of Clostridium acetobutylicum ATCC 824 using iTRAQ tags. Methods. 2013;61:269-76 pubmed publisher
    b>Clostridium acetobutylicum (Cac) is an anaerobic, endospore-forming, Gram-positive bacterium with tremendous promise for use as a biocatalyst for the production of fuels and solvents...
  5. Li R, Li Y, Lu L, Ren C, Li Y, Liu L. An improved kinetic model for the acetone-butanol-ethanol pathway of Clostridium acetobutylicum and model-based perturbation analysis. BMC Syst Biol. 2011;5 Suppl 1:S12 pubmed publisher
    ..b>Clostridium acetobutylicum produces solvents (acetone-butanol-ethanol, ABE) through the ABE pathway...
  6. Wang Q, Venkataramanan K, Huang H, Papoutsakis E, Wu C. Transcription factors and genetic circuits orchestrating the complex, multilayered response of Clostridium acetobutylicum to butanol and butyrate stress. BMC Syst Biol. 2013;7:120 pubmed publisher
  7. Ali M, Rudolph F, Bennett G. Characterization of thermostable Xyn10A enzyme from mesophilic Clostridium acetobutylicum ATCC 824. J Ind Microbiol Biotechnol. 2005;32:12-8 pubmed publisher
    A thermostable xylanase gene, xyn10A (CAP0053), was cloned from Clostridium acetobutylicum ATCC 824. The nucleotide sequence of the C...
  8. André G, Even S, Putzer H, Burguière P, Croux C, Danchin A, et al. S-box and T-box riboswitches and antisense RNA control a sulfur metabolic operon of Clostridium acetobutylicum. Nucleic Acids Res. 2008;36:5955-69 pubmed publisher
    The ubiGmccBA operon of Clostridium acetobutylicum is involved in methionine to cysteine conversion. We showed that its expression is controlled by a complex regulatory system combining several RNA-based mechanisms...
  9. Senger R, Papoutsakis E. Genome-scale model for Clostridium acetobutylicum: Part II. Development of specific proton flux states and numerically determined sub-systems. Biotechnol Bioeng. 2008;101:1053-71 pubmed publisher
    A regulated genome-scale model for Clostridium acetobutylicum ATCC 824 was developed based on its metabolic network reconstruction...
  10. Hillmann F, Fischer R, Saint Prix F, Girbal L, Bahl H. PerR acts as a switch for oxygen tolerance in the strict anaerobe Clostridium acetobutylicum. Mol Microbiol. 2008;68:848-60 pubmed publisher
    ..of the regulatory mechanism of this defence mechanism can trigger aerotolerance in the obligate anaerobe Clostridium acetobutylicum. Deletion of a peroxide repressor (PerR)-homologous protein resulted in prolonged aerotolerance, limited ..
  11. Jones S, Paredes C, Tracy B, Cheng N, Sillers R, Senger R, et al. The transcriptional program underlying the physiology of clostridial sporulation. Genome Biol. 2008;9:R114 pubmed publisher
    ..Elucidation of their sporulation program is critical for understanding important clostridial programs pertaining to their physiology and their industrial or environmental applications...
  12. Wu Y, Xue C, Chen L, Bai F. Effect of zinc supplementation on acetone-butanol-ethanol fermentation by Clostridium acetobutylicum. J Biotechnol. 2013;165:18-21 pubmed publisher
    In this article, effect of zinc supplementation on acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum was studied. It was found that when 0...
  13. Schwarz K, Kuit W, Grimmler C, Ehrenreich A, Kengen S. A transcriptional study of acidogenic chemostat cells of Clostridium acetobutylicum--cellular behavior in adaptation to n-butanol. J Biotechnol. 2012;161:366-77 pubmed publisher
    To gain more insight into the butanol stress response of Clostridium acetobutylicum the transcriptional response of a steady state acidogenic culture to different levels of n-butanol (0.25-1%) was investigated...
  14. Demuez M, Cournac L, Guerrini O, Soucaille P, Girbal L. Complete activity profile of Clostridium acetobutylicum [FeFe]-hydrogenase and kinetic parameters for endogenous redox partners. FEMS Microbiol Lett. 2007;275:113-21 pubmed publisher
    In Clostridium acetobutylicum, [FeFe]-hydrogenase is involved in hydrogen production in vivo by transferring electrons from physiological electron donors, ferredoxin and flavodoxin, to protons...
  15. Jiang Y, Xu C, Dong F, Yang Y, Jiang W, Yang S. Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio. Metab Eng. 2009;11:284-91 pubmed publisher
    ..The acetoacetate decarboxylase gene (adc) in the hyperbutanol-producing industrial strain Clostridium acetobutylicum EA 2018 was disrupted using TargeTron technology. The butanol ratio increased from 70% to 80...
  16. Khamaiseh E, Hamid A, Yusoff W, Kalil M. Effect of some environmental parameters on biobutanol production by Clostridium acetobutylicum NCIMB 13357 in date fruit medium. Pak J Biol Sci. 2013;16:1145-51 pubmed
    ..1, 0.1 and 1.1 g L(-1) butanol, ethanol and acetone respectively. The yield and productivity of biobutanol were 0.32 g g(-1) and 0.044 g L(-1)/h respectively, while for total ABE were 0.45 g g(-1) and 0.06 g L(-1) h, respectively. ..
  17. Mingardon F, Chanal A, Tardif C, Fierobe H. The issue of secretion in heterologous expression of Clostridium cellulolyticum cellulase-encoding genes in Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol. 2011;77:2831-8 pubmed publisher
    ..Taken together, our data suggest that a specific chaperone(s) involved in the secretion of the key family 48 cellulase, and probably Cel9G and Cel9E, is missing or insufficiently synthesized in C. acetobutylicum...
  18. Bao G, Wang R, Zhu Y, Dong H, Mao S, Zhang Y, et al. Complete genome sequence of Clostridium acetobutylicum DSM 1731, a solvent-producing strain with multireplicon genome architecture. J Bacteriol. 2011;193:5007-8 pubmed publisher
    b>Clostridium acetobutylicum is an important microorganism for solvent production. We report the complete genome sequence of C. acetobutylicum DSM 1731, a genome with multireplicon architecture. Comparison with the sequenced type strain C...
  19. Kawasaki S, Watamura Y, Ono M, Watanabe T, Takeda K, Niimura Y. Adaptive responses to oxygen stress in obligatory anaerobes Clostridium acetobutylicum and Clostridium aminovalericum. Appl Environ Microbiol. 2005;71:8442-50 pubmed
    b>Clostridium acetobutylicum and Clostridium aminovalericum, both obligatory anaerobes, grow normally after growth conditions are changed from anoxic to microoxic, where the cells consume oxygen proficiently. In C...
  20. Lee J, Jang Y, Lee J, Papoutsakis E, Lee S. Metabolic engineering of Clostridium acetobutylicum M5 for highly selective butanol production. Biotechnol J. 2009;4:1432-40 pubmed publisher
    To improve butanol selectivity, Clostridium acetobutylicum M5(pIMP1E1AB) was constructed by adhE1-ctfAB complementation of C. acetobutylicum M5, a derivative strain of C...
  21. Senger R, Papoutsakis E. Genome-scale model for Clostridium acetobutylicum: Part I. Metabolic network resolution and analysis. Biotechnol Bioeng. 2008;101:1036-52 pubmed publisher
    A genome-scale metabolic network reconstruction for Clostridium acetobutylicum (ATCC 824) was carried out using a new semi-automated reverse engineering algorithm...
  22. Xiao H, Gu Y, Ning Y, Yang Y, Mitchell W, Jiang W, et al. Confirmation and elimination of xylose metabolism bottlenecks in glucose phosphoenolpyruvate-dependent phosphotransferase system-deficient Clostridium acetobutylicum for simultaneous utilization of glucose, xylose, and arabinose. Appl Environ Microbiol. 2011;77:7886-95 pubmed publisher
    ..The Gram-positive anaerobic bacterium Clostridium acetobutylicum, known for its excellent capability of producing ABE (acetone, butanol, and ethanol) solvent, is limited ..
  23. Hu S, Zheng H, Gu Y, Zhao J, Zhang W, Yang Y, et al. Comparative genomic and transcriptomic analysis revealed genetic characteristics related to solvent formation and xylose utilization in Clostridium acetobutylicum EA 2018. BMC Genomics. 2011;12:93 pubmed publisher
    b>Clostridium acetobutylicum, a gram-positive and spore-forming anaerobe, is a major strain for the fermentative production of acetone, butanol and ethanol. But a previously isolated hyper-butanol producing strain C...
  24. Malca S, Girhard M, Schuster S, D rre P, Urlacher V. Expression, purification and characterization of two Clostridium acetobutylicum flavodoxins: potential electron transfer partners for CYP152A2. Biochim Biophys Acta. 2011;1814:257-64 pubmed publisher
    Two flavodoxin genes from Clostridium acetobutylicum, CacFld1 (CAC0587) and CacFld2 (CAC3417), were expressed in Escherichia coli and investigated for their ability to support activity of CYP152A2, a fatty acid hydroxylase from C...
  25. Crown S, Indurthi D, Ahn W, Choi J, Papoutsakis E, Antoniewicz M. Resolving the TCA cycle and pentose-phosphate pathway of Clostridium acetobutylicum ATCC 824: Isotopomer analysis, in vitro activities and expression analysis. Biotechnol J. 2011;6:300-5 pubmed publisher
    ..we utilized the power of (13) C-based isotopomer analysis to re-examine central metabolic pathways of Clostridium acetobutylicum ATCC 824...
  26. Inui M, Suda M, Kimura S, Yasuda K, Suzuki H, Toda H, et al. Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli. Appl Microbiol Biotechnol. 2008;77:1305-16 pubmed
    A recombinant butanol pathway composed of Clostridium acetobutylicum ATCC 824 genes, thiL, hbd, crt, bcd-etfB-etfA, and adhe1 (or adhe) coding for acetyl-CoA acetyltransferase (THL), beta-hydroxybutyryl-CoA dehydrogenase (HBD), 3-..
  27. Ho M, M n tret J, Tsuruta H, Allen K. The origin of the electrostatic perturbation in acetoacetate decarboxylase. Nature. 2009;459:393-7 pubmed publisher
  28. Servinsky M, Kiel J, Dupuy N, Sund C. Transcriptional analysis of differential carbohydrate utilization by Clostridium acetobutylicum. Microbiology. 2010;156:3478-91 pubmed publisher
    Transcriptional analysis was performed on Clostridium acetobutylicum with the goal of identifying sugar-specific mechanisms for the transcriptional regulation of transport and metabolism genes...
  29. Yen H, Chen Z, Yang I. Use of the composite membrane of poly(ether-block-amide) and carbon nanotubes (CNTs) in a pervaporation system incorporated with fermentation for butanol production by Clostridium acetobutylicum. Bioresour Technol. 2012;109:105-9 pubmed publisher
    ..In conclusion, the addition of CNTs to a PEBA pervaporation membrane has great potential when applied in the ABE fermentation industry...
  30. Faucher F, Wallace S, Doubli S. Structural basis for the lack of opposite base specificity of Clostridium acetobutylicum 8-oxoguanine DNA glycosylase. DNA Repair (Amst). 2009;8:1283-9 pubmed publisher
    ..A structural comparison with human OGG1 provides a rationale for the lack of opposite base specificity displayed by the bacterial Ogg...
  31. Jones S, Tracy B, Gaida S, Papoutsakis E. Inactivation of ?F in Clostridium acetobutylicum ATCC 824 blocks sporulation prior to asymmetric division and abolishes ?E and ?G protein expression but does not block solvent formation. J Bacteriol. 2011;193:2429-40 pubmed publisher
    b>Clostridium acetobutylicum is both a model organism for the understanding of sporulation in solventogenic clostridia and its relationship to solvent formation and an industrial organism for anaerobic acetone-butanol-ethanol (ABE) ..
  32. Xue C, Zhao J, Lu C, Yang S, Bai F, Tang I. High-titer n-butanol production by clostridium acetobutylicum JB200 in fed-batch fermentation with intermittent gas stripping. Biotechnol Bioeng. 2012;109:2746-56 pubmed publisher
    Acetone-butanol-ethanol (ABE) fermentation with a hyper-butanol producing Clostridium acetobutylicum JB200 was studied for its potential to produce a high titer of butanol that can be readily recovered with gas stripping...
  33. Kawasaki S, Ono M, Watamura Y, Sakai Y, Satoh T, Arai T, et al. An O2-inducible rubrerythrin-like protein, rubperoxin, is functional as a H2O2 reductase in an obligatory anaerobe Clostridium acetobutylicum. FEBS Lett. 2007;581:2460-4 pubmed
    b>Clostridium acetobutylicum, an obligatory anaerobe, is able to grow microoxically with the accumulation of two functionally unknown O2-induced proteins identified by two-dimensional electrophoresis...
  34. Mao S, Luo Y, Zhang T, Li J, Bao G, Zhu Y, et al. Proteome reference map and comparative proteomic analysis between a wild type Clostridium acetobutylicum DSM 1731 and its mutant with enhanced butanol tolerance and butanol yield. J Proteome Res. 2010;9:3046-61 pubmed publisher
    The solventogenic bacterium Clostridium acetobutylicum is an important species of the Clostridium community. To develop a fundamental tool that is useful for biological studies of C...
  35. Cai X, Bennett G. Improving the Clostridium acetobutylicum butanol fermentation by engineering the strain for co-production of riboflavin. J Ind Microbiol Biotechnol. 2011;38:1013-25 pubmed publisher
    ..investigate the possibility of co-production of a high value chemical during biobutanol production, the Clostridium acetobutylicum riboflavin operon ribGBAH was over-expressed in C...
  36. Schwarz K, Fiedler T, Fischer R, Bahl H. A Standard Operating Procedure (SOP) for the preparation of intra- and extracellular proteins of Clostridium acetobutylicum for proteome analysis. J Microbiol Methods. 2007;68:396-402 pubmed
    ..Operating Procedure (SOP) for extraction and handling of intra- and extracellular protein fractions of Clostridium acetobutylicum ATCC 824 for reproducible high quality two-dimensional gel electrophoresis (2-DE) analyses...
  37. Zhang Y, Han B, Ezeji T. Biotransformation of furfural and 5-hydroxymethyl furfural (HMF) by Clostridium acetobutylicum ATCC 824 during butanol fermentation. N Biotechnol. 2012;29:345-51 pubmed publisher
    ..The effect of furfural and HMF on butanol production by Clostridium acetobutylicum 824 was investigated. Whereas specific growth rates, ?, of C...
  38. Kuit W, Minton N, López Contreras A, Eggink G. Disruption of the acetate kinase (ack) gene of Clostridium acetobutylicum results in delayed acetate production. Appl Microbiol Biotechnol. 2012;94:729-41 pubmed publisher
    ..A mutant strain of Clostridium acetobutylicum lacking acetate kinase activity is expected to have reduced acetate and acetone production compared to ..
  39. Gao X, Zhao H, Zhang G, He K, Jin Y. Genome shuffling of Clostridium acetobutylicum CICC 8012 for improved production of acetone-butanol-ethanol (ABE). Curr Microbiol. 2012;65:128-32 pubmed publisher
    ..These results demonstrated the potential use of genome shuffling to microbial breeding which were difficult to deal with traditional methods...
  40. Gonzalez Pajuelo M, Meynial Salles I, Mendes F, Andrade J, Vasconcelos I, Soucaille P. Metabolic engineering of Clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerol. Metab Eng. 2005;7:329-36 pubmed
    ..quot;vitamin B12-free" biological process, we developed a metabolic engineering strategy with Clostridium acetobutylicum. The 1,3-propanediol pathway from C. butyricum was introduced on a plasmid in several mutants of C...
  41. Siemerink M, Kuit W, López Contreras A, Eggink G, van der Oost J, Kengen S. D-2,3-butanediol production due to heterologous expression of an acetoin reductase in Clostridium acetobutylicum. Appl Environ Microbiol. 2011;77:2582-8 pubmed publisher
    Acetoin reductase (ACR) catalyzes the conversion of acetoin to 2,3-butanediol. Under certain conditions, Clostridium acetobutylicum ATCC 824 (and strains derived from it) generates both d- and l-stereoisomers of acetoin, but because of ..
  42. Borden J, Papoutsakis E. Dynamics of genomic-library enrichment and identification of solvent tolerance genes for Clostridium acetobutylicum. Appl Environ Microbiol. 2007;73:3061-8 pubmed
    A Clostridium acetobutylicum ATCC 824 genomic library was constructed using randomly sheared DNA. Library inserts conferring increased tolerance to 1-butanol were isolated using two protocols...
  43. Janssen H, Grimmler C, Ehrenreich A, Bahl H, Fischer R. A transcriptional study of acidogenic chemostat cells of Clostridium acetobutylicum--solvent stress caused by a transient n-butanol pulse. J Biotechnol. 2012;161:354-65 pubmed publisher
    The main product of the anaerobic fermentative bacterium Clostridium acetobutylicum is n-butanol, an organic solvent with severe toxic effects on the cells...
  44. Jang Y, Woo H, Im J, Kim I, Lee S. Metabolic engineering of Clostridium acetobutylicum for enhanced production of butyric acid. Appl Microbiol Biotechnol. 2013;97:9355-63 pubmed publisher
    b>Clostridium acetobutylicum has been considered as an attractive platform host for biorefinery due to its metabolic diversity...
  45. Kutty R, Bennett G. Biochemical characterization of trinitrotoluene transforming oxygen-insensitive nitroreductases from Clostridium acetobutylicum ATCC 824. Arch Microbiol. 2005;184:158-67 pubmed publisher
    The genes that encode oxygen-insensitive nitroreductases from Clostridium acetobutylicum possessing 2,4,6-Trinitrotoluene (TNT) transformation activity were cloned, sequenced and characterized...
  46. Paredes C, Senger R, Spath I, Borden J, Sillers R, Papoutsakis E. A general framework for designing and validating oligomer-based DNA microarrays and its application to Clostridium acetobutylicum. Appl Environ Microbiol. 2007;73:4631-8 pubmed
    ..The 22K microarrays for the solvent producer Clostridium acetobutylicum ATCC 824 are based on in situ-synthesized 60-mers employing the Agilent technology...
  47. Sillers R, Chow A, Tracy B, Papoutsakis E. Metabolic engineering of the non-sporulating, non-solventogenic Clostridium acetobutylicum strain M5 to produce butanol without acetone demonstrate the robustness of the acid-formation pathways and the importance of the electron balance. Metab Eng. 2008;10:321-32 pubmed publisher
    ..The primary alcohol/aldehyde dehydrogenase (coded by the aad gene) is responsible for butanol formation in Clostridium acetobutylicum. We complemented the non-sporulating, non-solvent-producing C...
  48. Hönicke D, Janssen H, Grimmler C, Ehrenreich A, Lütke Eversloh T. Global transcriptional changes of Clostridium acetobutylicum cultures with increased butanol:acetone ratios. N Biotechnol. 2012;29:485-93 pubmed publisher
    ..on the molecular level were gained to explore the effect of methyl viologen addition to cultures of Clostridium acetobutylicum. Employing batch fermentation in mineral salts medium, the butanol:acetone ratio was successively ..
  49. Al Hinai M, Jones S, Papoutsakis E. ?K of Clostridium acetobutylicum is the first known sporulation-specific sigma factor with two developmentally separated roles, one early and one late in sporulation. J Bacteriol. 2014;196:287-99 pubmed publisher
    ..Here we show that the Clostridium acetobutylicum ?(K) acts both early, prior to Spo0A expression, and late, past ?(G) activation, thus departing from the ..
  50. Liu X, Gu Q, Yu X. Repetitive domestication to enhance butanol tolerance and production in Clostridium acetobutylicum through artificial simulation of bio-evolution. Bioresour Technol. 2013;130:638-43 pubmed publisher
    To improve butanol tolerance and production in Clostridium acetobutylicum, a novel approach was developed in this study, which was called artificial simulation of bio-evolution (ASBE) based on the evolutionary dynamics and natural ..
  51. Lee J, Yun H, Feist A, Palsson B, Lee S. Genome-scale reconstruction and in silico analysis of the Clostridium acetobutylicum ATCC 824 metabolic network. Appl Microbiol Biotechnol. 2008;80:849-62 pubmed publisher
    To understand the metabolic characteristics of Clostridium acetobutylicum and to examine the potential for enhanced butanol production, we reconstructed the genome-scale metabolic network from its annotated genomic sequence and analyzed ..
  52. King P, Posewitz M, Ghirardi M, Seibert M. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system. J Bacteriol. 2006;188:2163-72 pubmed
    ..coli by cloning and coexpression of hydE, hydF, and hydG from the bacterium Clostridium acetobutylicum. Coexpression of the C...
  53. Wang S, Zhu Y, Zhang Y, Li Y. Controlling the oxidoreduction potential of the culture of Clostridium acetobutylicum leads to an earlier initiation of solventogenesis, thus increasing solvent productivity. Appl Microbiol Biotechnol. 2012;93:1021-30 pubmed publisher
    Fermentative production of solvents (acetone, butanol, and ethanol) by Clostridium acetobutylicum is generally a biphasic process consisting of acidogenesis and solventogenesis. We report that the biphasic metabolism of C...
  54. Grimmler C, Janssen H, Krausse D, Fischer R, Bahl H, Dürre P, et al. Genome-wide gene expression analysis of the switch between acidogenesis and solventogenesis in continuous cultures of Clostridium acetobutylicum. J Mol Microbiol Biotechnol. 2011;20:1-15 pubmed publisher
    b>Clostridium acetobutylicum is able to switch from acidogenic growth to solventogenic growth. We used phosphate-limited continuous cultures that established acidogenic growth at pH 5.8 and solventogenic growth at pH 4.5...
  55. Grimmler C, Held C, Liebl W, Ehrenreich A. Transcriptional analysis of catabolite repression in Clostridium acetobutylicum growing on mixtures of D-glucose and D-xylose. J Biotechnol. 2010;150:315-23 pubmed publisher
    b>Clostridium acetobutylicum is a strict anaerobic organism that is used for biotechnological butanol fermentation. It ferments various hexoses and pentoses to solvents but prefers glucose presumably using a catabolite repression mechanism...
  56. Robey Bond S, Barrantes Reynolds R, Bond J, Wallace S, Bandaru V. Clostridium acetobutylicum 8-oxoguanine DNA glycosylase (Ogg) differs from eukaryotic Oggs with respect to opposite base discrimination. Biochemistry. 2008;47:7626-36 pubmed publisher
    ..We report the first characterization of a bacterial Ogg, Clostridium acetobutylicum Ogg (CacOgg). Like human OGG1 and Escherichia coli Fpg (EcoFpg), CacOgg excised 8-oxoguanine...
  57. Alsaker K, Papoutsakis E. Transcriptional program of early sporulation and stationary-phase events in Clostridium acetobutylicum. J Bacteriol. 2005;187:7103-18 pubmed
    DNA microarray analysis of Clostridium acetobutylicum was used to examine the genomic-scale gene expression changes during the shift from exponential-phase growth and acidogenesis to stationary phase and solventogenesis...
  58. Sillers R, Al Hinai M, Papoutsakis E. Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations. Biotechnol Bioeng. 2009;102:38-49 pubmed publisher
    Metabolic engineering (ME) of Clostridium acetobutylicum has led to increased solvent (butanol, acetone, and ethanol) production and solvent tolerance, thus demonstrating that further efforts have the potential to create strains of ..
  59. Borden J, Jones S, Indurthi D, Chen Y, Papoutsakis E. A genomic-library based discovery of a novel, possibly synthetic, acid-tolerance mechanism in Clostridium acetobutylicum involving non-coding RNAs and ribosomal RNA processing. Metab Eng. 2010;12:268-81 pubmed publisher
    We generated a genomic library from sheared Clostridium acetobutylicum ATCC 824 DNA, whereby inserts can be expressed in both directions from the thiolase promoter, P(thl). Serial transfer of library-bearing C...
  60. Zhang H, Bruns M, Logan B. Biological hydrogen production by Clostridium acetobutylicum in an unsaturated flow reactor. Water Res. 2006;40:728-34 pubmed
    ..The reactor consisted of a column packed with glass beads and inoculated with a pure culture (Clostridium acetobutylicum ATCC 824). A defined medium containing glucose was fed at a flow rate of 1.6 mL/min (0...
  61. Faucher F, Robey Bond S, Wallace S, Doubli S. Structural characterization of Clostridium acetobutylicum 8-oxoguanine DNA glycosylase in its apo form and in complex with 8-oxodeoxyguanosine. J Mol Biol. 2009;387:669-79 pubmed publisher
    ..While Clostridium acetobutylicum Ogg (CacOgg) DNA glycosylase can specifically recognize and remove 8-oxoG, it displays little preference ..
  62. Zhu L, Dong H, Zhang Y, Li Y. Engineering the robustness of Clostridium acetobutylicum by introducing glutathione biosynthetic capability. Metab Eng. 2011;13:426-34 pubmed publisher
    To improve the aero- and solvent tolerance of the solvent-producing Clostridium acetobutylicum, glutathione biosynthetic capability was introduced into C...
  63. Alsaker K, Paredes C, Papoutsakis E. Metabolite stress and tolerance in the production of biofuels and chemicals: gene-expression-based systems analysis of butanol, butyrate, and acetate stresses in the anaerobe Clostridium acetobutylicum. Biotechnol Bioeng. 2010;105:1131-47 pubmed publisher
    ..Using DNA microarray-based gene expression analysis, the transcriptional-stress responses of Clostridium acetobutylicum to fermentation acids acetate and butyrate and the solvent product butanol were analyzed and compared in ..
  64. Fiedler T, Mix M, Meyer U, Mikkat S, Glocker M, Bahl H, et al. The two-component system PhoPR of Clostridium acetobutylicum is involved in phosphate-dependent gene regulation. J Bacteriol. 2008;190:6559-67 pubmed publisher
    The phoPR gene locus of Clostridium acetobutylicum ATCC 824 comprises two genes, phoP and phoR. Deduced proteins are predicted to represent a response regulator and sensor kinase of a phosphate-dependent two-component regulatory system...
  65. Guerrini O, Burlat B, L ger C, Guigliarelli B, Soucaille P, Girbal L. Characterization of two 2[4Fe4S] ferredoxins from Clostridium acetobutylicum. Curr Microbiol. 2008;56:261-7 pubmed publisher
    In vivo hydrogen production in Clostridium acetobutylicum involves electron transfer between ferredoxin and [FeFe]-hydrogenase. Five C. acetobutylicum open reading frames were annotated as coding for putative ferredoxins...
  66. Emmerechts G, Barbé S, Herdewijn P, Anne J, Rozenski J. Post-transcriptional modification mapping in the Clostridium acetobutylicum 16S rRNA by mass spectrometry and reverse transcriptase assays. Nucleic Acids Res. 2007;35:3494-503 pubmed
    ..The present study describes the characterization of the post-transcriptional modifications in Clostridium acetobutylicum 16S rRNA, using high-pressure liquid chromatography (HPLC) coupled to electrospray ionization mass ..
  67. Lütke Eversloh T, Bahl H. Metabolic engineering of Clostridium acetobutylicum: recent advances to improve butanol production. Curr Opin Biotechnol. 2011;22:634-47 pubmed publisher
    ..Among solventogenic clostridia, Clostridium acetobutylicum represents the model organism and general but yet important genetic tools were established only recently ..
  68. Hambourger M, Gervaldo M, Svedruzic D, King P, Gust D, Ghirardi M, et al. [FeFe]-hydrogenase-catalyzed H2 production in a photoelectrochemical biofuel cell. J Am Chem Soc. 2008;130:2015-22 pubmed publisher
    The Clostridium acetobutylicum [FeFe]-hydrogenase HydA has been investigated as a hydrogen production catalyst in a photoelectrochemical biofuel cell. Hydrogenase was adsorbed to pyrolytic graphite edge and carbon felt electrodes...
  69. Riebe O, Fischer R, Bahl H. Desulfoferrodoxin of Clostridium acetobutylicum functions as a superoxide reductase. FEBS Lett. 2007;581:5605-10 pubmed publisher
    Desulfoferrodoxin (cac2450) of Clostridium acetobutylicum was purified after overexpression in E. coli. In an in vitro assay the enzyme exhibited superoxide reductase activity with rubredoxin (cac2778) of C...
  70. Zhang L, Leyn S, Gu Y, Jiang W, Rodionov D, Yang C. Ribulokinase and transcriptional regulation of arabinose metabolism in Clostridium acetobutylicum. J Bacteriol. 2012;194:1055-64 pubmed publisher
    ..in nine Clostridium species with detailed experimental characterization of AraR-mediated regulation in Clostridium acetobutylicum. Based on the reconstructed AraR regulons, a novel ribulokinase, AraK, present in all analyzed ..
  71. Bi C, Jones S, Hess D, Tracy B, Papoutsakis E. SpoIIE is necessary for asymmetric division, sporulation, and expression of sigmaF, sigmaE, and sigmaG but does not control solvent production in Clostridium acetobutylicum ATCC 824. J Bacteriol. 2011;193:5130-7 pubmed publisher
    ..past Spo0A activation and the associated solventogenesis in the important industrial and model organism Clostridium acetobutylicum, the spoIIE gene was successfully disrupted and its expression was silenced...
  72. Hou X, Peng W, Xiong L, Huang C, Chen X, Chen X, et al. Engineering Clostridium acetobutylicum for alcohol production. J Biotechnol. 2013;166:25-33 pubmed publisher
    While Clostridium acetobutylicum has been used for large-scale butanol production (ABE fermentation), its by-product acetone cannot be used as a biofuel. In this study, C...
  73. Lehmann D, Radomski N, Lütke Eversloh T. New insights into the butyric acid metabolism of Clostridium acetobutylicum. Appl Microbiol Biotechnol. 2012;96:1325-39 pubmed publisher
    ..of acetone and n-butanol is naturally restricted to the group of solventogenic clostridia with Clostridium acetobutylicum being the model organism for acetone-butanol-ethanol (ABE) fermentation...
  74. Steiner E, Scott J, Minton N, Winzer K. An agr quorum sensing system that regulates granulose formation and sporulation in Clostridium acetobutylicum. Appl Environ Microbiol. 2012;78:1113-22 pubmed publisher
    The Gram-positive, anaerobic, endospore-forming bacterium Clostridium acetobutylicum has considerable biotechnological potential due to its ability to produce solvents as fermentation products, in particular the biofuel butanol...
  75. Min K, Kim S, Yum T, Kim Y, Sang B, Um Y. Conversion of levulinic acid to 2-butanone by acetoacetate decarboxylase from Clostridium acetobutylicum. Appl Microbiol Biotechnol. 2013;97:5627-34 pubmed publisher
    ..Acetoacetate decarboxylase (AADC; E.C. 4.1.1.4) from Clostridium acetobutylicum was selected as a biocatalyst for decarboxylation of levulinic acid...
  76. Dong H, Zhang Y, Dai Z, Li Y. Engineering clostridium strain to accept unmethylated DNA. PLoS ONE. 2010;5:e9038 pubmed publisher
    ..The strategy reported here makes it easy to genetically modify the clostridial species using unmethylated DNA, which will help to advance the understanding of the clostridial physiology from the molecular level...
  77. Steiner E, Dago A, Young D, Heap J, Minton N, Hoch J, et al. Multiple orphan histidine kinases interact directly with Spo0A to control the initiation of endospore formation in Clostridium acetobutylicum. Mol Microbiol. 2011;80:641-54 pubmed publisher
    The phosphorylated Spo0A transcription factor controls the initiation of endospore formation in Clostridium acetobutylicum, but genes encoding key phosphorelay components, Spo0F and Spo0B, are missing in the genome...
  78. Gu Y, Hu S, Chen J, Shao L, He H, Yang Y, et al. Ammonium acetate enhances solvent production by Clostridium acetobutylicum EA 2018 using cassava as a fermentation medium. J Ind Microbiol Biotechnol. 2009;36:1225-32 pubmed publisher
    ..to the cassava medium significantly promotes solvent production from cassava fermented by Clostridium acetobutylicum EA 2018, a mutant with a high butanol ratio...