geobacillus stearothermophilus

Summary

Summary: A species of GRAM-POSITIVE ENDOSPORE-FORMING BACTERIA in the family BACILLACEAE, found in soil, hot springs, Arctic waters, ocean sediments, and spoiled food products.

Top Publications

  1. Winter G, Fersht A, Wilkinson A, Zoller M, Smith M. Redesigning enzyme structure by site-directed mutagenesis: tyrosyl tRNA synthetase and ATP binding. Nature. 1982;299:756-8 pubmed
  2. Sleytr U, Beveridge T. Bacterial S-layers. Trends Microbiol. 1999;7:253-60 pubmed
    ..Some archaeal and eubacterial S-layer proteins are glycosylated. These lattices typically have center-to-center spacings of less than 25 nm, which makes them attractive for biomimetic or nanotechnological applications. ..
  3. Li Y, Naveen V, Lin M, Hsiao C. Structural analyses of the bacterial primosomal protein DnaB reveal that it is a tetramer and forms a complex with a primosomal re-initiation protein. J Biol Chem. 2017;292:15744-15757 pubmed publisher
    ..crystal structure of DnaB, encompassing the N-terminal and middle domains (residues 1-300), from Geobacillus stearothermophilus (GstDnaB1-300) at 2.8 Å resolution...
  4. Logan N, Berkeley R. Identification of Bacillus strains using the API system. J Gen Microbiol. 1984;130:1871-82 pubmed
    ..A taxonomy based upon API tests is in good agreement with those obtained by other methods. The results matrix can also be used in computer assisted identification. ..
  5. Baba T, Shinke R, Nanmori T. Identification and characterization of clustered genes for thermostable xylan-degrading enzymes, beta-xylosidase and xylanase, of Bacillus stearothermophilus 21. Appl Environ Microbiol. 1994;60:2252-8 pubmed
    ..The two genes thus appear to be, at least partly, expressed independently, which was experimentally confirmed in E. coli by deletion analysis.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  6. Rife J, Moore P. The structure of a methylated tetraloop in 16S ribosomal RNA. Structure. 1998;6:747-56 pubmed
    ..It is likely that the conformation of this loop is crucial for its function, having implications for its interaction with ribosomal subunits and its role in the initiation of protein synthesis. ..
  7. Pei H, Han S, Yang S, Lei Z, Zheng J, Jia Z. Phosphorylation of bacterial L9 and its functional implication in response to starvation stress. FEBS Lett. 2017;591:3421-3430 pubmed publisher
  8. Huang Z, Liu X, Zhang S, Liu Z. GH52 xylosidase from Geobacillus stearothermophilus: characterization and introduction of xylanase activity by site?directed mutagenesis of Tyr509. J Ind Microbiol Biotechnol. 2014;41:65-74 pubmed
    A xylosidase gene, gsxyn, was cloned from the deep-sea thermophilic Geobacillus stearothermophilus, which consisted of 2,118 bp and encoded a protein of 705 amino acids with a calculated molecular mass of 79.8 kDa...
  9. Sanders K, Lin C, Smith A, Cronin N, Fisher G, Eftychidis V, et al. The structure and function of an RNA polymerase interaction domain in the PcrA/UvrD helicase. Nucleic Acids Res. 2017;45:3875-3887 pubmed publisher
    ..The implications of this work for our understanding of the role of PcrA/UvrD at the interface of DNA replication, transcription and repair are discussed. ..

More Information

Publications19

  1. Li Z, Su L, Duan X, Wu D, Wu J. Efficient Expression of Maltohexaose-Forming ?-Amylase from Bacillus stearothermophilus in Brevibacillus choshinensis SP3 and Its Use in Maltose Production. Biomed Res Int. 2017;2017:5479762 pubmed publisher
    ..This study demonstrated that the B. choshinensis SP3 expression system was able to produce substantial quantities of recombinant ?-amylase that has potential application in the starch industry. ..
  2. Sara M, Sleytr U. Charge distribution on the S layer of Bacillus stearothermophilus NRS 1536/3c and importance of charged groups for morphogenesis and function. J Bacteriol. 1987;169:2804-9 pubmed
    ..Among other functions, the biological significance of the S layers may be in masking the electronegative charge of the cell wall proper. ..
  3. Oscorbin I, Belousova E, Boyarskikh U, Zakabunin A, Khrapov E, Filipenko M. Derivatives of Bst-like Gss-polymerase with improved processivity and inhibitor tolerance. Nucleic Acids Res. 2017;45:9595-9610 pubmed publisher
    ..Therefore, chimeric Bst-like Gss-polymerase will be promising tool for both WGA and POCT due to increased processivity and inhibitor tolerance. ..
  4. Naitow H, Matsuura Y, Tono K, Joti Y, Kameshima T, Hatsui T, et al. Protein-ligand complex structure from serial femtosecond crystallography using soaked thermolysin microcrystals and comparison with structures from synchrotron radiation. Acta Crystallogr D Struct Biol. 2017;73:702-709 pubmed publisher
    ..In a comparison of the SFX and SR structures, clear differences were observed in the unit-cell parameters, in the alternate conformation of side chains, in the degree of water coordination and in the ligand-binding mode. ..
  5. Kakagianni M, Aguirre J, Lianou A, Koutsoumanis K. Effect of storage temperature on the lag time of Geobacillus stearothermophilus individual spores. Food Microbiol. 2017;67:76-84 pubmed publisher
    The lag times (?) of Geobacillus stearothermophilus single spores were studied at different storage temperatures ranging from 45 to 59 °C using the Bioscreen C method...
  6. Kim M, Kim H, Lee J, Park S, Oh T. Thermostable lipase of Bacillus Stearothermophilus: high-level production, purification, and calcium-dependent thermostability. Biosci Biotechnol Biochem. 2000;64:280-6 pubmed
  7. Brown P, Richardson C, Mensah L, O Hanlon P, Osborne N, Pope A, et al. Molecular recognition of tyrosinyl adenylate analogues by prokaryotic tyrosyl tRNA synthetases. Bioorg Med Chem. 1999;7:2473-85 pubmed
    ..The inhibition data has been further supported by homology modelling with the S. aureus enzyme, and by ligand docking studies. ..
  8. Liu Y, Huang W, Huang T, Kunin C. Detection of antimicrobial activity in urine for epidemiologic studies of antibiotic use. J Clin Epidemiol. 1999;52:539-45 pubmed
    ..4% and specificity of 94.9%. Detection of antimicrobial activity in urine is a promising method to determine antimicrobial drug use in epidemiologic studies, particularly in populations in which drug use history is unreliable. ..
  9. Sara M, Pum D, Kupcu S, Messner P, Sleytr U. Isolation of two physiologically induced variant strains of Bacillus stearothermophilus NRS 2004/3a and characterization of their S-layer lattices. J Bacteriol. 1994;176:848-60 pubmed
    ..Peptide mapping and N-terminal sequencing results strongly indicated that the three S-layer proteins are encoded by different genes and are not derived from a universal precursor form. ..
  10. Ikai A. Thermostability and aliphatic index of globular proteins. J Biochem. 1980;88:1895-8 pubmed
    ..The index may be regarded as a positive factor for the increase of thermostability of globular proteins. ..