bacillus megaterium

Summary

Summary: A species of bacteria whose spores vary from round to elongate. It is a common soil saprophyte.

Top Publications

  1. Chowdhary P, Keshavan N, Nguyen H, Peterson J, GONZALEZ J, Haines D. Bacillus megaterium CYP102A1 oxidation of acyl homoserine lactones and acyl homoserines. Biochemistry. 2007;46:14429-37 pubmed
    ..In this work we report that CYP102A1, a widely studied cytochrome P450 from Bacillus megaterium, is capable of very efficient oxidation of AHLs and their lactonolysis products acyl homoserines...
  2. Takagi Y, Sugisawa T, Hoshino T. Continuous 2-Keto-L-gulonic acid fermentation by mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium or Xanthomonas maltophilia. Appl Microbiol Biotechnol. 2010;86:469-80 pubmed publisher
    ..The mixed culture of Ketogulonicigenium vulgare DSM 4025 and Bacillus megaterium DSM 4026 produced 90 g/L of 2KGA from 120 g/L of L-sorbose at the dilution rate of 0...
  3. Mishra R, Prajapati S, Das J, Dangar T, Das N, Thatoi H. Reduction of selenite to red elemental selenium by moderately halotolerant Bacillus megaterium strains isolated from Bhitarkanika mangrove soil and characterization of reduced product. Chemosphere. 2011;84:1231-7 pubmed publisher
    ..16S rDNA sequence analysis of BSB6 and BSB12 showed the closest phylogenetic similarity with the species Bacillus megaterium. Both the strains effectively reduced Se(IV) and complete reduction of selenite (up to 0...
  4. Zhang J, Zhou J, Liu J, Chen K, Liu L, Chen J. Development of chemically defined media supporting high cell density growth of Ketogulonicigenium vulgare and Bacillus megaterium. Bioresour Technol. 2011;102:4807-14 pubmed publisher
    ..This is commonly produced commercially by Ketogulonicigenium vulgare and Bacillus megaterium, using corn steep liquor powder (CSLP) as an organic nitrogen source...
  5. Malten M, Biedendieck R, Gamer M, Drews A, Stammen S, Buchholz K, et al. A Bacillus megaterium plasmid system for the production, export, and one-step purification of affinity-tagged heterologous levansucrase from growth medium. Appl Environ Microbiol. 2006;72:1677-9 pubmed
    A multiple vector system for the production and export of recombinant affinity-tagged proteins in Bacillus megaterium was developed...
  6. Kleser M, Hannemann F, Hutter M, Zapp J, Bernhardt R. CYP105A1 mediated 3-hydroxylation of glimepiride and glibenclamide using a recombinant Bacillus megaterium whole-cell catalyst. J Biotechnol. 2012;157:405-12 pubmed publisher
    ..For high scale production of sulfonylurea derivatives, we designed whole-cell biocatalysts based on Bacillus megaterium MS941...
  7. Thavasi R, Jayalakshmi S, Banat I. Effect of biosurfactant and fertilizer on biodegradation of crude oil by marine isolates of Bacillus megaterium, Corynebacterium kutscheri and Pseudomonas aeruginosa. Bioresour Technol. 2011;102:772-8 pubmed publisher
    ..of fertilizers and biosurfactants on biodegradation of crude oil by three marine bacterial isolates; Bacillus megaterium, Corynebacterium kutscheri and Pseudomonas aeruginosa...
  8. Eiben S, Bartelmäs H, Urlacher V. Construction of a thermostable cytochrome P450 chimera derived from self-sufficient mesophilic parents. Appl Microbiol Biotechnol. 2007;75:1055-61 pubmed
    The P450 monooxygenases CYP102A1 from Bacillus megaterium and CYP102A3 from Bacillus subtilis are fusion flavocytochromes comprising of a P450 heme domain and a FAD/FMN reductase domain...
  9. Vary P, Biedendieck R, Fuerch T, Meinhardt F, Rohde M, Deckwer W, et al. Bacillus megaterium--from simple soil bacterium to industrial protein production host. Appl Microbiol Biotechnol. 2007;76:957-67 pubmed
    b>Bacillus megaterium has been industrially employed for more than 50 years, as it possesses some very useful and unusual enzymes and a high capacity for the production of exoenzymes...

More Information

Publications73

  1. Kong Q, Shan S, Liu Q, Wang X, Yu F. Biocontrol of Aspergillus flavus on peanut kernels by use of a strain of marine Bacillus megaterium. Int J Food Microbiol. 2010;139:31-5 pubmed publisher
    A strain of marine Bacillus megaterium isolated from the Yellow Sea of East China was evaluated for its activity in reducing postharvest decay of peanut kernels caused by Aspergillus flavus in in vitro and in vivo tests...
  2. David F, Westphal R, Bunk B, Jahn D, Franco Lara E. Optimization of antibody fragment production in Bacillus megaterium: the role of metal ions on protein secretion. J Biotechnol. 2010;150:115-24 pubmed publisher
    ..3 scFv in Bacillus megaterium YYBm1...
  3. Bäumchen C, Roth A, Biedendieck R, Malten M, Follmann M, Sahm H, et al. D-mannitol production by resting state whole cell biotrans-formation of D-fructose by heterologous mannitol and formate dehydrogenase gene expression in Bacillus megaterium. Biotechnol J. 2007;2:1408-16 pubmed
    ..of the gene mdh encoding mannitol dehydrogenase (MDH) from Leuconostoc pseudomesenteroides ATCC12291 in Bacillus megaterium. The NADH reduction equivalents necessary for MDH activity were regenerated via the oxidation of formate to ..
  4. Yun C, Kim K, Kim D, Jung H, Pan J. The bacterial P450 BM3: a prototype for a biocatalyst with human P450 activities. Trends Biotechnol. 2007;25:289-98 pubmed
    ..CYP102A1 (P450 BM3) from Bacillus megaterium integrates an entire monooxygenase system into one polypeptide and represents an appropriate prokaryotic ..
  5. Kiss A, Balikó G, Csorba A, Chuluunbaatar T, Medzihradszky K, Alföldi L. Cloning and characterization of the DNA region responsible for Megacin A-216 production in Bacillus megaterium 216. J Bacteriol. 2008;190:6448-57 pubmed publisher
    Upon induction, Bacillus megaterium 216 produces the bacteriocin megacin A-216, which leads to lysis of the producer cell and kills B. megaterium and a few other bacterial species...
  6. Chowdhury S, Basak R, Sen R, Adhikari B. Production of extracellular polysaccharide by Bacillus megaterium RB-05 using jute as substrate. Bioresour Technol. 2011;102:6629-32 pubmed publisher
    b>Bacillus megaterium RB-05 was grown on glucose and on "tossa-daisee" (Corchorus olitorius)-derived jute, and production and composition of extracellular polysaccharide (EPS) were monitored. An EPS yield of 0.065 ± 0...
  7. Malten M, Hollmann R, Deckwer W, Jahn D. Production and secretion of recombinant Leuconostoc mesenteroides dextransucrase DsrS in Bacillus megaterium. Biotechnol Bioeng. 2005;89:206-18 pubmed
    Leuconostoc mesenteroides dextransucrase DsrS was recombinantly produced in Bacillus megaterium and exported into the growth medium...
  8. David F, Berger A, Hansch R, Rohde M, Franco Lara E. Single cell analysis applied to antibody fragment production with Bacillus megaterium: development of advanced physiology and bioprocess state estimation tools. Microb Cell Fact. 2011;10:23 pubmed publisher
  9. Christie G, Lazarevska M, Lowe C. Functional consequences of amino acid substitutions to GerVB, a component of the Bacillus megaterium spore germinant receptor. J Bacteriol. 2008;190:2014-22 pubmed publisher
    ..predicted to lie in loop regions of GerVB on the exterior aspect of the membrane, that influence the Bacillus megaterium spore germination response...
  10. Lee T, Lin J, Wang S, Shaw G. PhaQ, a new class of poly-beta-hydroxybutyrate (phb)-responsive repressor, regulates phaQ and phaP (phasin) expression in Bacillus megaterium through interaction with PHB. J Bacteriol. 2004;186:3015-21 pubmed
    b>Bacillus megaterium can produce poly-beta-hydroxybutyrate (PHB) as carbon and energy storage materials...
  11. Rea V, Kolkman A, Vottero E, Stronks E, Ampt K, Honing M, et al. Active site substitution A82W improves the regioselectivity of steroid hydroxylation by cytochrome P450 BM3 mutants as rationalized by spin relaxation nuclear magnetic resonance studies. Biochemistry. 2012;51:750-60 pubmed publisher
    Cytochrome P450 BM3 from Bacillus megaterium is a monooxygenase with great potential for biotechnological applications...
  12. Fleming B, Tian Y, Bell S, Wong L, Urlacher V, Hill H. Redox properties of cytochrome p450BM3 measured by direct methods. Eur J Biochem. 2003;270:4082-8 pubmed
    ..The reduced heme rapidly catalysed the reduction of oxygen to hydrogen peroxide...
  13. Sato T, Hoshino H, Yoshida S, Nakajima M, Hoshino T. Bifunctional triterpene/sesquarterpene cyclase: tetraprenyl-?-curcumene cyclase is also squalene cyclase in Bacillus megaterium. J Am Chem Soc. 2011;133:17540-3 pubmed publisher
    ..This is the first report describing a bifunctional terpene cyclase, which biosynthesizes two classes of cyclic terpenes with different numbers of carbons as natural products in the organism...
  14. Radha S, Gunasekaran P. Cloning and expression of keratinase gene in Bacillus megaterium and optimization of fermentation conditions for the production of keratinase by recombinant strain. J Appl Microbiol. 2007;103:1301-10 pubmed
    Cloning and expression of keratinase gene in Bacillus megaterium and optimization of fermentation conditions for the production of keratinase by recombinant strain.
  15. Shuster V, Fishman A. Isolation, cloning and characterization of a tyrosinase with improved activity in organic solvents from Bacillus megaterium. J Mol Microbiol Biotechnol. 2009;17:188-200 pubmed publisher
    ..Amplification of the 16S rDNA genes revealed a high similarity with Bacillus megaterium. The enzyme was over-expressed in Escherichia coli BL21 and purified using an affinity column...
  16. Eppinger M, Bunk B, Johns M, Edirisinghe J, Kutumbaka K, Koenig S, et al. Genome sequences of the biotechnologically important Bacillus megaterium strains QM B1551 and DSM319. J Bacteriol. 2011;193:4199-213 pubmed publisher
    b>Bacillus megaterium is deep-rooted in the Bacillus phylogeny, making it an evolutionarily key species and of particular importance in understanding genome evolution, dynamics, and plasticity in the bacilli. B...
  17. Coleman W, Zhang P, Li Y, Setlow P. Mechanism of killing of spores of Bacillus cereus and Bacillus megaterium by wet heat. Lett Appl Microbiol. 2010;50:507-14 pubmed publisher
    To determine the mechanism of wet heat killing of spores of Bacillus cereus and Bacillus megaterium.
  18. Radha S, Gunasekaran P. Sustained expression of keratinase gene under PxylA and PamyL promoters in the recombinant Bacillus megaterium MS941. Bioresour Technol. 2008;99:5528-37 pubmed
    ..megaterium MS941 (pWHK3) grown for 24h. The recombinant plasmids were stably maintained with sustained expression of keratinase for about 60 generations in B. megaterium MS941 rather than in B. megaterium 1,4945...
  19. Chen H, Swenson R. Effect of the Insertion of a Glycine Residue into the Loop Spanning Residues 536-541 on the Semiquinone State and Redox Properties of the Flavin Mononucleotide-Binding Domain of Flavocytochrome P450BM-3 from Bacillus megaterium. Biochemistry. 2008;47:13788-99 pubmed publisher
    ..These results demonstrate the importance of the unique structural characteristics of the shorter loop in P450BM-3 in establishing the unique redox properties of the FMN in this protein but not its general cytochrome reductase activity...
  20. Homann A, Biedendieck R, Götze S, Jahn D, Seibel J. Insights into polymer versus oligosaccharide synthesis: mutagenesis and mechanistic studies of a novel levansucrase from Bacillus megaterium. Biochem J. 2007;407:189-98 pubmed
    A novel levansucrase was identified in the supernatant of a cell culture of Bacillus megaterium DSM319...
  21. Christie G, Lowe C. Role of chromosomal and plasmid-borne receptor homologues in the response of Bacillus megaterium QM B1551 spores to germinants. J Bacteriol. 2007;189:4375-83 pubmed
    Spores of Bacillus megaterium QM B1551 germinate in response to a number of trigger compounds, including glucose, proline, leucine, and inorganic salts...
  22. Patil A, K P, Mulimani V, Veeranagouda Y, Lee K. alpha-Galactosidase from Bacillus megaterium VHM1 and its application in removal of flatulence-causing factors from soymilk. J Microbiol Biotechnol. 2010;20:1546-54 pubmed
    ..Furthermore, 16S rDNA sequence analysis of new isolates was identified as Bacillus megaterium VHM1. The production of alpha-galactosidase was optimized by various physical culture conditions...
  23. Strube C, Homann A, Gamer M, Jahn D, Seibel J, Heinz D. Polysaccharide synthesis of the levansucrase SacB from Bacillus megaterium is controlled by distinct surface motifs. J Biol Chem. 2011;286:17593-600 pubmed publisher
    ..The kinetic and biochemical characterization of Bacillus megaterium levansucrase SacB variants Y247A, Y247W, N252A, D257A, and K373A reveal novel surface motifs remote from ..
  24. Lüders S, David F, Steinwand M, Jordan E, Hust M, Dubel S, et al. Influence of the hydromechanical stress and temperature on growth and antibody fragment production with Bacillus megaterium. Appl Microbiol Biotechnol. 2011;91:81-90 pubmed publisher
    b>Bacillus megaterium was used for production of the lysozyme-specific recombinant scFv D1.3 antibody fragment...
  25. Malten M, Nahrstedt H, Meinhardt F, Jahn D. Coexpression of the type I signal peptidase gene sipM increases recombinant protein production and export in Bacillus megaterium MS941. Biotechnol Bioeng. 2005;91:616-21 pubmed
    ..In order to improve Bacillus megaterium as protein production and secretion host, the influence of homologous type I signal peptidase SipM ..
  26. Chowdhury S, Manna S, Saha P, Basak R, Sen R, Roy D, et al. Composition analysis and material characterization of an emulsifying extracellular polysaccharide (EPS) produced by Bacillus megaterium RB-05: a hydrodynamic sediment-attached isolate of freshwater origin. J Appl Microbiol. 2011;111:1381-93 pubmed publisher
    ..to isolate, purify and characterize an extracellular polysaccharide (EPS) produced by a freshwater dynamic sediment-attached micro-organism, Bacillus megaterium RB-05, and study its emulsifying potential in different hydrocarbon media.
  27. Biedendieck R, Malten M, Barg H, Bunk B, Martens J, Deery E, et al. Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microb Biotechnol. 2010;3:24-37 pubmed publisher
    Cobalamin (vitamin B(12)) production in Bacillus megaterium has served as a model system for the systematic evaluation of single and multiple directed molecular and genetic optimization strategies...
  28. Sawayama A, Chen M, Kulanthaivel P, Kuo M, Hemmerle H, Arnold F. A panel of cytochrome P450 BM3 variants to produce drug metabolites and diversify lead compounds. Chemistry. 2009;15:11723-9 pubmed publisher
    Herein we demonstrate that a small panel of variants of cytochrome P450 BM3 from Bacillus megaterium covers the breadth of reactivity of human P450s by producing 12 of 13 mammalian metabolites for two marketed drugs, verapamil and ..
  29. Saravanan M, Vemu A, Barik S. Rapid biosynthesis of silver nanoparticles from Bacillus megaterium (NCIM 2326) and their antibacterial activity on multi drug resistant clinical pathogens. Colloids Surf B Biointerfaces. 2011;88:325-31 pubmed publisher
    ..present study is focused on the extracellular biosynthesis of highly stable Ag-BNPs from bacterial strain Bacillus megaterium (NCIM 2326) by bio-reduction of silver ion using the culture supernatant, and to determine the ..
  30. Lin Z, Xue R, Ye Y, Zheng J, Xu Z. A further insight into the biosorption mechanism of Pt(IV) by infrared spectrometry. BMC Biotechnol. 2009;9:62 pubmed publisher
  31. Ustok F, Packman L, Lowe C, Christie G. Spore germination mediated by Bacillus megaterium QM B1551 SleL and YpeB. J Bacteriol. 2014;196:1045-54 pubmed publisher
    Previous work demonstrated that Bacillus megaterium QM B1551 spores that are null for the sleB and cwlJ genes, which encode cortex-lytic enzymes (CLEs), either of which is required for efficient cortex hydrolysis in Bacillus spores, ..
  32. Sun J, Wang W, Hundertmark C, Zeng A, Jahn D, Deckwer W. A protein database constructed from low-coverage genomic sequence of Bacillus megaterium and its use for accelerated proteomic analysis. J Biotechnol. 2006;124:486-95 pubmed
    ..We recently started to sequence the genome of Bacillus megaterium DSM 319, a bacterium of industrial interest...
  33. Schubert H, Rose R, Leech H, Brindley A, Hill C, Rigby S, et al. Structure and function of SirC from Bacillus megaterium: a metal-binding precorrin-2 dehydrogenase. Biochem J. 2008;415:257-63 pubmed publisher
    In Bacillus megaterium, the synthesis of vitamin B(12) (cobalamin) and sirohaem diverges at sirohydrochlorin along the branched modified tetrapyrrole biosynthetic pathway...
  34. Burger S, Tatge H, Hofmann F, Genth H, Just I, Gerhard R. Expression of recombinant Clostridium difficile toxin A using the Bacillus megaterium system. Biochem Biophys Res Commun. 2003;307:584-8 pubmed
    Pathogenic Clostridium difficile produces two major protein toxins, toxin A and toxin B. We used the Bacillus megaterium expression system for expression of recombinant toxin A...
  35. Biedendieck R, Gamer M, Jaensch L, Meyer S, Rohde M, Deckwer W, et al. A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium. J Biotechnol. 2007;132:426-30 pubmed
    A sucrose-inducible promoter system (P(sacB)) from Bacillus megaterium was identified using a secretome approach. It was successfully employed for the extracellular production of the homologous levansucrase SacB (4252...
  36. Pueyo M, Bloch C, Carmona Ribeiro A, Di Mascio P. Lipopeptides produced by a soil Bacillus megaterium strain. Microb Ecol. 2009;57:367-78 pubmed publisher
    ..Raw mixtures of all these compounds were also assayed for antibiotic features. The data enlighten the unusual diversity of the lipopeptide mixture produced by a sole Bacillus species...
  37. Bleif S, Hannemann F, Zapp J, Hartmann D, Jauch J, Bernhardt R. A new Bacillus megaterium whole-cell catalyst for the hydroxylation of the pentacyclic triterpene 11-keto-?-boswellic acid (KBA) based on a recombinant cytochrome P450 system. Appl Microbiol Biotechnol. 2012;93:1135-46 pubmed publisher
    ..The prokaryotic cytochrome P450 CYP106A2 from Bacillus megaterium ATCC 13368 hydroxylates a variety of 3-oxo-?? steroids and recently it was identified to carry out a one-..
  38. Budde M, Morr M, Schmid R, Urlacher V. Selective hydroxylation of highly branched fatty acids and their derivatives by CYP102A1 from Bacillus megaterium. Chembiochem. 2006;7:789-94 pubmed
    ..CYP102A1 from Bacillus megaterium and its A74G F87V L188Q triple mutant hydroxylate a variety of these substrates with high activity and ..
  39. Wang W, Sun J, Hollmann R, Zeng A, Deckwer W. Proteomic characterization of transient expression and secretion of a stress-related metalloprotease in high cell density culture of Bacillus megaterium. J Biotechnol. 2006;126:313-24 pubmed
    ..and mass spectrometric analysis (2DE/MS) for high cell density fed-batch culture of recombinant Bacillus megaterium strains. In the early feeding phase with a constant growth rate of 0...
  40. Biedendieck R, Yang Y, Deckwer W, Malten M, Jahn D. Plasmid system for the intracellular production and purification of affinity-tagged proteins in Bacillus megaterium. Biotechnol Bioeng. 2007;96:525-37 pubmed
    ..vector system for the intracellular high-level production of affinity tagged recombinant proteins in Bacillus megaterium was developed...
  41. Schmitz D, Zapp J, Bernhardt R. Hydroxylation of the triterpenoid dipterocarpol with CYP106A2 from Bacillus megaterium. FEBS J. 2012;279:1663-74 pubmed publisher
    The bacterial steroid-hydroxylase CYP106A2 from Bacillus megaterium ATCC 13368 hydroxylates a variety of 3-oxo-?-4-steroids and has recently been shown to catalyse regioselective hydroxylation of the diterpene abietic acid, as well as ..
  42. Huang W, Westlake A, Marechal J, Joyce M, Moody P, Roberts G. Filling a hole in cytochrome P450 BM3 improves substrate binding and catalytic efficiency. J Mol Biol. 2007;373:633-51 pubmed
    Cytochrome P450BM3 (CYP102A1) from Bacillus megaterium, a fatty acid hydroxylase, is a member of a very large superfamily of monooxygenase enzymes...
  43. Chen L, Chen W. Isolation and characterization of a novel small antifungal peptide from Bacillus megaterium D4 Isolated from the dung of wild plateau yak in China. Protein Pept Lett. 2010;17:542-6 pubmed
    ..antifungal peptide-producing strain D4 was isolated from the dung of wild plateau yak and identified as Bacillus megaterium. The purification procedure of peptide consisted of acid precipitation, methanol extract and C(18) reverse-..
  44. Gamer M, Fröde D, Biedendieck R, Stammen S, Jahn D. A T7 RNA polymerase-dependent gene expression system for Bacillus megaterium. Appl Microbiol Biotechnol. 2009;82:1195-203 pubmed publisher
    ..During the last decade, the Gram-positive bacterium Bacillus megaterium was established as a useful host for the intra- and extracellular production of heterologous proteins...
  45. Peters M, Meinhold P, Glieder A, Arnold F. Regio- and enantioselective alkane hydroxylation with engineered cytochromes P450 BM-3. J Am Chem Soc. 2003;125:13442-50 pubmed
    Cytochrome P450 BM-3 from Bacillus megaterium was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant...
  46. Yang Y, Malten M, Grote A, Jahn D, Deckwer W. Codon optimized Thermobifida fusca hydrolase secreted by Bacillus megaterium. Biotechnol Bioeng. 2007;96:780-94 pubmed
    Production and secretion of a 28,172 Da hydrolase from Thermobifida fusca (TFH) in Bacillus megaterium MS941 and WH323 was investigated in shake flask and pH controlled bioreactors...
  47. Chakraborty U, Chakraborty B, Basnet M. Plant growth promotion and induction of resistance in Camellia sinensis by Bacillus megaterium. J Basic Microbiol. 2006;46:186-95 pubmed
    b>Bacillus megaterium DE BARY TRS-4 was isolated from tea rhizosphere and tested for its ability to promote growth and cause disease reduction in tea plants...
  48. Schewe H, Holtmann D, Schrader J. P450(BM-3)-catalyzed whole-cell biotransformation of alpha-pinene with recombinant Escherichia coli in an aqueous-organic two-phase system. Appl Microbiol Biotechnol. 2009;83:849-57 pubmed publisher
  49. Huang C, Chen M, Hsieh J, Lin W, Chen P, Chien L. Expression of mercuric reductase from Bacillus megaterium MB1 in eukaryotic microalga Chlorella sp. DT: an approach for mercury phytoremediation. Appl Microbiol Biotechnol. 2006;72:197-205 pubmed publisher
    A eukaryotic microalga, Chlorella sp. DT, was transformed with the Bacillus megaterium strain MB1 merA gene, encoding mercuric reductase (MerA), which mediates the reduction of Hg2+ to volatile elemental Hg0...
  50. Baik S, Michel F, Aghajari N, Haser R, Harayama S. Cooperative effect of two surface amino acid mutations (Q252L and E170K) in glucose dehydrogenase from Bacillus megaterium IWG3 on stabilization of its oligomeric state. Appl Environ Microbiol. 2005;71:3285-93 pubmed
    A thermostable glucose dehydrogenase (GlcDH) mutant of Bacillus megaterium IWG3 harboring the Q252L substitution (Y. Makino, S. Negoro, I. Urabe, and H. Okada, J. Biol. Chem...
  51. Nahrstedt H, Wittchen K, Rachman M, Meinhardt F. Identification and functional characterization of a type I signal peptidase gene of Bacillus megaterium DSM319. Appl Microbiol Biotechnol. 2004;64:243-9 pubmed publisher
    The sipM gene of Bacillus megaterium encoding a type I signal peptidase (SPase) was isolated and structurally characterized...
  52. Yang G, Zhou B, Wang J, He X, Sun X, Nie W, et al. Expression of recombinant Clostridium difficile toxin A and B in Bacillus megaterium. BMC Microbiol. 2008;8:192 pubmed publisher
    ..Major Clostridium difficile virulence factors are the exotoxins TcdA and TcdB. Due to the large size and poor stability of the proteins, the active recombinant TcdA and TcdB have been difficult to produce...
  53. Hollmann R, Deckwer W. Pyruvate formation and suppression in recombinant Bacillus megaterium cultivation. J Biotechnol. 2004;111:89-96 pubmed
    A recombinant Bacillus megaterium strain showed the ability to secrete large amounts of pyruvate (up to 27.8 gl( -1)) for growth rates larger than 0.15 h(-1)...
  54. Raux E, Leech H, Beck R, Schubert H, Santander P, Roessner C, et al. Identification and functional analysis of enzymes required for precorrin-2 dehydrogenation and metal ion insertion in the biosynthesis of sirohaem and cobalamin in Bacillus megaterium. Biochem J. 2003;370:505-16 pubmed
    In Bacillus megaterium, the hemAXBCDL genes were isolated and were found to be highly similar to the genes from Bacillus subtilis that are required for the conversion of glutamyl-tRNA into uroporphyrinogen III...
  55. Yao X, Khan F, Pandey R, Pandey J, Mourant R, Jain R, et al. Degradation of dichloroaniline isomers by a newly isolated strain, Bacillus megaterium IMT21. Microbiology. 2011;157:721-6 pubmed publisher
    ..This bacterium, Bacillus megaterium IMT21, also mineralized 2,3-, 2,4-, 2,5- and 3,5-DCA as sole sources of carbon and energy...
  56. Zou C, Li Z, Yu D. Bacillus megaterium strain XTBG34 promotes plant growth by producing 2-pentylfuran. J Microbiol. 2010;48:460-6 pubmed publisher
    ..strain XTBG34, was isolated from a Xishuangbanna Tropical Botanical Garden soil sample and identified as Bacillus megaterium. The strain's volatiles had remarkable plant growth promotion activity in Arabidopsis thaliana plants; ..
  57. Biedendieck R, Beine R, Gamer M, Jordan E, Buchholz K, Seibel J, et al. Export, purification, and activities of affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium. Appl Microbiol Biotechnol. 2007;74:1062-73 pubmed
    ..enzyme were recombinantly produced and exported into the growth medium using the Gram-positive bacterium Bacillus megaterium. Nutrient-rich growth medium significantly enhanced levansucrase production and export. The B...
  58. Marulanda A, Azcón R, Chaumont F, Ruiz Lozano J, Aroca R. Regulation of plasma membrane aquaporins by inoculation with a Bacillus megaterium strain in maize (Zea mays L.) plants under unstressed and salt-stressed conditions. Planta. 2010;232:533-43 pubmed publisher
    ..Here, maize (Zea mays L.) plants were inoculated with a Bacillus megaterium strain previously isolated from a degraded soil and characterized as PGPR...
  59. Pandian S, Deepak V, Kalishwaralal K, Rameshkumar N, Jeyaraj M, Gurunathan S. Optimization and fed-batch production of PHB utilizing dairy waste and sea water as nutrient sources by Bacillus megaterium SRKP-3. Bioresour Technol. 2010;101:705-11 pubmed publisher
    ..properties and nucleotide sequence of its 16S rRNA, it was suggested that strain SRKP-3 was similar to Bacillus megaterium. A four-factor central composite rotary design (CCRD) was employed to optimize the medium and to find out ..
  60. Gerhard R, Burger S, Tatge H, Genth H, Just I, Hofmann F. Comparison of wild type with recombinant Clostridium difficile toxin A. Microb Pathog. 2005;38:77-83 pubmed
    ..We, therefore, expressed recombinant His-tagged toxin A using the Bacillus megaterium expression system. Specific antisera raised against the native toxin A from C...
  61. Warman A, Roitel O, Neeli R, Girvan H, Seward H, Murray S, et al. Flavocytochrome P450 BM3: an update on structure and mechanism of a biotechnologically important enzyme. Biochem Soc Trans. 2005;33:747-53 pubmed
    ..in the 1980s, the fatty acid hydroxylase flavocytochrome P450 (cytochrome P450) BM3 (CYP102A1) from Bacillus megaterium has been adopted as a paradigm for the understanding of structure and mechanism in the P450 superfamily of ..
  62. Vazquez G, Pettinari M, Méndez B. Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium. Int Microbiol. 2003;6:127-9 pubmed
    Molecular analysis of a genomic region of Bacillus megaterium, a polyhydroxybutyrate (PHB)-producing microorganism, revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb)...
  63. Fürch T, Wittmann C, Wang W, Franco Lara E, Jahn D, Deckwer W. Effect of different carbon sources on central metabolic fluxes and the recombinant production of a hydrolase from Thermobifida fusca in Bacillus megaterium. J Biotechnol. 2007;132:385-94 pubmed
    The recombinant Bacillus megaterium strain WH323 was employed for the inducible production and secretion of recombinant Thermobifida fusca hydrolase (TFH)...
  64. Kanteev M, Goldfeder M, Chojnacki M, Adir N, Fishman A. The mechanism of copper uptake by tyrosinase from Bacillus megaterium. J Biol Inorg Chem. 2013;18:895-903 pubmed publisher
    ..We have recently determined the crystal structure of tyrosinase from Bacillus megaterium (TyrBm) and revealed that this enzyme has tighter binding of CuA in comparison with CuB...