rhodothermus

Summary

Summary: A genus of obligately aerobic, thermophilic, gram-negative bacteria in the family Crenotrichaceae. They were isolated from submarine alkaline HOT SPRINGS in Iceland.

Top Publications

  1. Stelter M, Melo A, Pereira M, Gomes C, Hreggvidsson G, Hjorleifsdottir S, et al. A novel type of monoheme cytochrome c: biochemical and structural characterization at 1.23 A resolution of rhodothermus marinus cytochrome c. Biochemistry. 2008;47:11953-63 pubmed publisher
    ..23 A resolution, of a monoheme cytochrome c from the thermohalophilic bacterium Rhodothermus marinus...
  2. Yoon S, Ryu S, Lee S, Moon T. Purification and characterization of branching specificity of a novel extracellular amylolytic enzyme from marine hyperthermophilic Rhodothermus marinus. J Microbiol Biotechnol. 2008;18:457-64 pubmed
    ..enzyme (RMEBE) possessing alpha- (1-->4)-(1-->6)-transferring activity was purified to homogeneity from Rhodothermus marinus by combination of ammonium sulfate precipitation, Q-Sepharose ion-exchange, and Superdex- 200 gel ..
  3. Wang X, Yang H, Ruan L, Liu X, Li F, Xu X. Cloning and characterization of a thermostable superoxide dismutase from the thermophilic bacterium Rhodothermus sp. XMH10. J Ind Microbiol Biotechnol. 2008;35:133-9 pubmed
    A superoxide dismutase (SOD) gene was cloned from the thermophilic bacterium Rhodothermus sp. XMH10 for the first time and highly expressed in Escherichia coli. The Rhodothermus sp...
  4. Abou Hachem M, Olsson F, Nordberg Karlsson E. Probing the stability of the modular family 10 xylanase from Rhodothermus marinus. Extremophiles. 2003;7:483-91 pubmed
    The thermophilic bacterium Rhodothermus marinus produces a modular xylanase (Xyn10A) consisting of two N-terminal carbohydrate-binding modules (CBMs), followed by a domain of unknown function, and a catalytic module flanked by a fifth ..
  5. Borges N, Marugg J, Empadinhas N, da Costa M, Santos H. Specialized roles of the two pathways for the synthesis of mannosylglycerate in osmoadaptation and thermoadaptation of Rhodothermus marinus. J Biol Chem. 2004;279:9892-8 pubmed
    b>Rhodothermus marinus responds to fluctuations in the growth temperature and/or salinity by accumulating mannosylglycerate (MG)...
  6. Bjornsdottir S, Fridjonsson O, Kristjansson J, Eggertsson G. Cloning and expression of heterologous genes in Rhodothermus marinus. Extremophiles. 2007;11:283-93 pubmed
    The construction of a shuttle cloning system for Rhodothermus marinus and Escherichia coli is described...
  7. Srinivasan V, Rajendran C, Sousa F, Melo A, Saraiva L, Pereira M, et al. Structure at 1.3 A resolution of Rhodothermus marinus caa(3) cytochrome c domain. J Mol Biol. 2005;345:1047-57 pubmed
    The cytochrome c domain of subunit II from the Rhodothermus marinus caa(3) HiPIP:oxygen oxidoreductase, a member of the superfamily of heme-copper-containing terminal oxidases, was produced in Escherichia coli and characterised...
  8. Pereira M, Sousa F, Teixeira M, Nyquist R, Heberle J. A tyrosine residue deprotonates during oxygen reduction by the caa3 reductase from Rhodothermus marinus. FEBS Lett. 2006;580:1350-4 pubmed
    ..ATR-FTIR) difference spectroscopy that a tyrosine residue of the oxygen reductase from the thermohalophilic Rhodothermus marinus becomes deprotonated in the transition from the oxidized state to the catalytic intermediate ferryl ..
  9. Batista A, Fernandes A, Louro R, Steuber J, Pereira M. Energy conservation by Rhodothermus marinus respiratory complex I. Biochim Biophys Acta. 2010;1797:509-15 pubmed publisher
    ..efflux, together with a proton influx and an inside-positive DeltaPsi, was observed during NADH-respiration by Rhodothermus marinus membrane vesicles...

More Information

Publications81

  1. Bleicher L, Prates E, Gomes T, Silveira R, Nascimento A, Rojas A, et al. Molecular basis of the thermostability and thermophilicity of laminarinases: X-ray structure of the hyperthermostable laminarinase from Rhodothermus marinus and molecular dynamics simulations. J Phys Chem B. 2011;115:7940-9 pubmed publisher
    ..Here, we present X-ray structure of the hyperthermophilic laminarinase from Rhodothermus marinus (RmLamR) determined at 1...
  2. Batista A, Marreiros B, Pereira M. Decoupling of the catalytic and transport activities of complex I from Rhodothermus marinus by sodium/proton antiporter inhibitor. ACS Chem Biol. 2011;6:477-83 pubmed publisher
    ..The decoupling of the catalytic and transport activities suggests the involvement of an indirect coupling mechanism, possibly through conformational changes...
  3. Gunnarsson L, Zhou Q, Montanier C, Karlsson E, Brumer H, Ohlin M. Engineered xyloglucan specificity in a carbohydrate-binding module. Glycobiology. 2006;16:1171-80 pubmed
    ..proteins from a combinatorial library of the carbohydrate-binding module, CBM4-2, from xylanase Xyn10A of Rhodothermus marinus is described...
  4. Crennell S, Cook D, Minns A, Svergun D, Andersen R, Nordberg Karlsson E. Dimerisation and an increase in active site aromatic groups as adaptations to high temperatures: X-ray solution scattering and substrate-bound crystal structures of Rhodothermus marinus endoglucanase Cel12A. J Mol Biol. 2006;356:57-71 pubmed
    ..The bacterium Rhodothermus marinus, isolated from shallow water marine hot springs, produces a number of carbohydrate-degrading enzymes ..
  5. Soares C, Baptista A, Pereira M, Teixeira M. Investigation of protonatable residues in Rhodothermus marinus caa3 haem-copper oxygen reductase: comparison with Paracoccus denitrificans aa3 haem-copper oxygen reductase. J Biol Inorg Chem. 2004;9:124-34 pubmed
    The Rhodothermus marinus caa(3 )haem-copper oxygen reductase contains all the residues of the so-called D- and K-proton channels, with the notable exception of the helix VI glutamate residue (Glu278(I) in Paracoccus denitrificans aa(3)), ..
  6. Cicortas Gunnarsson L, Nordberg Karlsson E, Albrekt A, Andersson M, Holst O, Ohlin M. A carbohydrate binding module as a diversity-carrying scaffold. Protein Eng Des Sel. 2004;17:213-21 pubmed
    ..study, we investigated the use of a thermostable carbohydrate binding module, CBM4-2, from a xylanase found in Rhodothermus marinus, as a diversity-carrying scaffold...
  7. Bjornsdottir S, Thorbjarnardottir S, Eggertsson G. Establishment of a gene transfer system for Rhodothermus marinus. Appl Microbiol Biotechnol. 2005;66:675-82 pubmed
    Genetic manipulation of Rhodothermus marinus has been hampered by the lack of a selection system for gene transfer. We report construction of a Rhodothermus/Escherichia coli shuttle plasmid, containing the R...
  8. Pereira M, Refojo P, Hreggvidsson G, Hjorleifsdottir S, Teixeira M. The alternative complex III from Rhodothermus marinus - a prototype of a new family of quinol:electron acceptor oxidoreductases. FEBS Lett. 2007;581:4831-5 pubmed
    The biochemical and genetic search for a bc(1) complex in Rhodothermus marinus was always fruitless; however, a functional equivalent, i.e. having quinol:cytochrome c oxidoreductase activity was characterized...
  9. Veríssimo A, Pereira M, Melo A, Hreggvidsson G, Kristjansson J, Teixeira M. A ba3 oxygen reductase from the thermohalophilic bacterium Rhodothermus marinus. FEMS Microbiol Lett. 2007;269:41-7 pubmed
    The aerobic respiratory chain of the thermohalophilic bacterium Rhodothermus marinus has been extensively studied. In this study the isolation and characterization of a third oxygen reductase expressed in this organism are described...
  10. Jorge C, Sampaio M, Hreggvidsson G, Kristjánson J, Santos H. A highly thermostable trehalase from the thermophilic bacterium Rhodothermus marinus. Extremophiles. 2007;11:115-22 pubmed
    ..A periplasmic trehalase (alpha,alpha-trehalose glucohydrolase, EC 3.2.1.28) from the thermophilic bacterium Rhodothermus marinus was purified and the respective encoding gene was identified, cloned and overexpressed in Escherichia ..
  11. Bjornsdottir S, Blondal T, Hreggvidsson G, Eggertsson G, Petursdottir S, Hjorleifsdottir S, et al. Rhodothermus marinus: physiology and molecular biology. Extremophiles. 2006;10:1-16 pubmed
    b>Rhodothermus marinus has been the subject of many studies in recent years. It is a thermohalophilic bacterium and is the only validly described species in the genus Rhodothermus...
  12. Refojo P, Teixeira M, Pereira M. The alternative complex III of Rhodothermus marinus and its structural and functional association with caa3 oxygen reductase. Biochim Biophys Acta. 2010;1797:1477-82 pubmed publisher
    ..ACIII from Rhodothermus (R...
  13. Hobel C, Hreggvidsson G, Marteinsson V, Bahrani Mougeot F, Einarsson J, Kristjansson J. Cloning, expression, and characterization of a highly thermostable family 18 chitinase from Rhodothermus marinus. Extremophiles. 2005;9:53-64 pubmed
    A family 18 chitinase gene chiA from the thermophile Rhodothermus marinus was cloned and expressed in Escherichia coli...
  14. Cicortas Gunnarsson L, Montanier C, Tunnicliffe R, Williamson M, Gilbert H, Nordberg Karlsson E, et al. Novel xylan-binding properties of an engineered family 4 carbohydrate-binding module. Biochem J. 2007;406:209-14 pubmed
    ..Here, we report the engineering of a CBM, CBM4-2 from the Rhodothermus marinus xylanase Xyn10A, and the identification of the X-2 variant...
  15. Duan X, Lu A, Gu B, Cai Z, Ma H, Wei S, et al. Functional characterization of the UDP-xylose biosynthesis pathway in Rhodothermus marinus. Appl Microbiol Biotechnol. 2015;99:9463-72 pubmed publisher
    ..b>Rhodothermus marinus, a halothermophilic bacterium commonly found in hot springs, proved to be a valuable source of ..
  16. Sekirnik R, Wilkins S, Bush J, Tarhonskaya H, Münzel M, Hussein A, et al. YcfDRM is a thermophilic oxygen-dependent ribosomal protein uL16 oxygenase. Extremophiles. 2018;22:553-562 pubmed publisher
    ..Bioinformatic analysis identified a potential homologue of ycfD in the thermophilic bacterium Rhodothermus marinus (ycfDRM)...
  17. Bae J, Kim I, Kim K, Nam K. Crystal structure of a substrate-binding protein from Rhodothermus marinus reveals a single α/β-domain. Biochem Biophys Res Commun. 2018;497:368-373 pubmed publisher
    ..Here, we report the crystal structure of an unusually small SBP from Rhodothermus marinus (named as RmSBP) at 1.9 Å...
  18. Mathew S, Aronsson A, Karlsson E, Adlercreutz P. Xylo- and arabinoxylooligosaccharides from wheat bran by endoxylanases, utilisation by probiotic bacteria, and structural studies of the enzymes. Appl Microbiol Biotechnol. 2018;102:3105-3120 pubmed publisher
    ..glycoside hydrolase (GH) family 10 xylanases GsXyn10A from Geobacillus stearothermophilus and RmXyn10A-CM from Rhodothermus marinus produced the AXOS A3X, A2XX and A2?+?3XX in addition to XOS...
  19. Okanishi H, Kim K, Masui R, Kuramitsu S. Proteome-wide identification of lysine propionylation in thermophilic and mesophilic bacteria: Geobacillus kaustophilus, Thermus thermophilus, Escherichia coli, Bacillus subtilis, and Rhodothermus marinus. Extremophiles. 2017;21:283-296 pubmed publisher
    ..bacteria: Geobacillus kaustophilus, Thermus thermophilus, Escherichia coli, Bacillus subtilis, and Rhodothermus marinus...
  20. Refojo P, Calisto F, Ribeiro M, Teixeira M, Pereira M. The monoheme cytochrome c subunit of Alternative Complex III is a direct electron donor to caa3 oxygen reductase in Rhodothermus marinus. Biol Chem. 2017;398:1037-1044 pubmed publisher
    ..We expressed and characterized the individually isolated ActE, which allowed us to suggest that ActE is a lipoprotein and to show its function as a direct electron donor to the caa3 oxygen reductase. ..
  21. Okanishi H, Kim K, Fukui K, Yano T, Kuramitsu S, Masui R. Proteome-wide identification of lysine succinylation in thermophilic and mesophilic bacteria. Biochim Biophys Acta Proteins Proteom. 2017;1865:232-242 pubmed publisher
    ..were identified in Thermus thermophilus, and low succinylation and propionylation were observed in Rhodothermus marinus...
  22. Golubev A, Rojas A, Nascimento A, Bleicher L, Kulminskaya A, Eneyskaya E, et al. Crystallization and preliminary crystallographic analysis of laminarinase from Rhodothermus marinus: a case of pseudomerohedral twinning. Protein Pept Lett. 2008;15:1142-4 pubmed
    Thermophilic endo-1,3(4)-beta glucanase (laminarinase) from Rhodothermus marinus was crystallized by the hanging-drop vapor diffusion method...
  23. Sorndech W, Sagnelli D, Meier S, Jansson A, Lee B, Hamaker B, et al. Structure of branching enzyme- and amylomaltase modified starch produced from well-defined amylose to amylopectin substrates. Carbohydr Polym. 2016;152:51-61 pubmed publisher
    Thermostable branching enzyme (BE, EC 2.4.1.18) from Rhodothermus obamensis in combination with amylomaltase (AM, EC 2.4.1...
  24. Ron E, Plaza M, Kristjansdottir T, Sardari R, Bjornsdottir S, Gudmundsson S, et al. Characterization of carotenoids in Rhodothermus marinus. Microbiologyopen. 2017;: pubmed publisher
    b>Rhodothermus marinus, a marine aerobic thermophile, was first isolated from an intertidal hot spring in Iceland. In recent years, the R...
  25. Mathew S, Karlsson E, Adlercreutz P. Extraction of soluble arabinoxylan from enzymatically pretreated wheat bran and production of short xylo-oligosaccharides and arabinoxylo-oligosaccharides from arabinoxylan by glycoside hydrolase family 10 and 11 endoxylanases. J Biotechnol. 2017;260:53-61 pubmed publisher
    ..XOS) and arabinoxylo-oligosaccharides (AXOS) (53% w/w) followed by the catalytic module of Rhodothermus marinus Xyn10A (RmXyn10A-CM) with 37% (w/w) conversion...
  26. Kapoor D, Kumar V, Chandrayan S, Ahmed S, Sharma S, Datt M, et al. Replacement of the active surface of a thermophile protein by that of a homologous mesophile protein through structure-guided 'protein surface grafting'. Biochim Biophys Acta. 2008;1784:1771-6 pubmed publisher
    ..a beta sheet functioning as the substrate-binding and catalytically-active groove of a thermophile cellulase (Rhodothermus marinus Cel12A) to cause it to resemble, both in its structure and function, the equivalent groove of a ..
  27. Kan S, Lewis R, Chen K, Arnold F. Directed evolution of cytochrome c for carbon-silicon bond formation: Bringing silicon to life. Science. 2016;354:1048-1051 pubmed
    ..Using directed evolution, we enhanced the catalytic function of cytochrome c from Rhodothermus marinus to achieve more than 15-fold higher turnover than state-of-the-art synthetic catalysts...
  28. Wagschal K, Rose Stoller J, Chan V, Jordan D. Expression and Characterization of Hyperthermostable Exopolygalacturonase RmGH28 from Rhodothermus marinus. Appl Biochem Biotechnol. 2017;183:1503-1515 pubmed publisher
    The gene RmGH28 from the organism Rhodothermus marinus, a putative glycosyl hydrolase family 28 polygalacturonase, was expressed in Escherichia coli and biochemically characterized...
  29. Cordova L, Cipolla R, Swarup A, Long C, Antoniewicz M. 13C metabolic flux analysis of three divergent extremely thermophilic bacteria: Geobacillus sp. LC300, Thermus thermophilus HB8, and Rhodothermus marinus DSM 4252. Metab Eng. 2017;44:182-190 pubmed publisher
    ..LC300, Thermus thermophilus HB8, and Rhodothermus marinus DSM 4252...
  30. Kan S, Huang X, Gumulya Y, Chen K, Arnold F. Genetically programmed chiral organoborane synthesis. Nature. 2017;: pubmed publisher
    ..Escherichia coli cells harbouring wild-type cytochrome c from Rhodothermus marinus (Rma cyt c) were found to form carbon-boron bonds in the presence of borane-Lewis base complexes, ..
  31. Saburi W, Jaito N, Kato K, Tanaka Y, Yao M, Mori H. Biochemical and structural characterization of Marinomonas mediterranead-mannose isomerase Marme_2490 phylogenetically distant from known enzymes. Biochimie. 2018;144:63-73 pubmed publisher
    ..and substrate binding by Marme_2490 were similar to those in both AKI (Salmonella enterica AKI) and epimerase (Rhodothermus marinus CE)...
  32. Flint J, Taylor E, Yang M, Bolam D, Tailford L, Martinez Fleites C, et al. Structural dissection and high-throughput screening of mannosylglycerate synthase. Nat Struct Mol Biol. 2005;12:608-14 pubmed
    ..we report broad high-throughput screening and kinetic analyses of both natural and synthetic substrates of Rhodothermus marinus mannosylglycerate synthase (MGS), which catalyzes the formation of the stress protectant 2-O-alpha-D-..
  33. Sardari R, Kulcinskaja E, Ron E, Björnsdóttir S, Friðjónsson O, Hreggviðsson G, et al. Evaluation of the production of exopolysaccharides by two strains of the thermophilic bacterium Rhodothermus marinus. Carbohydr Polym. 2017;156:1-8 pubmed publisher
    The thermophile Rhodothermus marinus produces extracellular polysaccharides (EPSs) that forms a distinct cellular capsule. Here, the first data on EPS production in strains DSM4252T and MAT493 are reported and compared...
  34. Karlsson E, Hachem M, Ramchuran S, Costa H, Holst O, Fex Svenningsen A, et al. The modular xylanase Xyn10A from Rhodothermus marinus is cell-attached, and its C-terminal domain has several putative homologues among cell-attached proteins within the phylum Bacteroidetes. FEMS Microbiol Lett. 2004;241:233-42 pubmed
    Until recently, the function of the fifth domain of the thermostable modular xylanase Xyn10A from Rhodothermus marinus was unresolved...
  35. Bergal H, Hopkins A, Metzner S, Sousa M. The Structure of a BamA-BamD Fusion Illuminates the Architecture of the β-Barrel Assembly Machine Core. Structure. 2016;24:243-51 pubmed publisher
    ..Here we present the crystal structure of a fusion between BamA POTRA4-5 and BamD from Rhodothermus marinus. The POTRA5 domain binds BamD between its tetratricopeptide repeats 3 and 4...
  36. Gunnarsson L, Dexlin L, Karlsson E, Holst O, Ohlin M. Evolution of a carbohydrate binding module into a protein-specific binder. Biomol Eng. 2006;23:111-7 pubmed
    A carbohydrate binding module, CBM4-2, derived from the xylanase (Xyn 10A) of Rhodothermus marinus has been used as a scaffold for molecular diversification...
  37. Molinas M, De Candia A, Szajnman S, Rodriguez J, Marti M, Pereira M, et al. Electron transfer dynamics of Rhodothermus marinus caa3 cytochrome c domains on biomimetic films. Phys Chem Chem Phys. 2011;13:18088-98 pubmed publisher
    The subunit II of the caa(3) oxygen reductase from Rhodothermus marinus contains, in addition to the Cu(A) center, a c-type heme group in the cytochrome c domain (Cyt-D) that is the putative primary electron acceptor of the enzyme...
  38. Ye X, Zhu Z, Zhang C, Zhang Y. Fusion of a family 9 cellulose-binding module improves catalytic potential of Clostridium thermocellum cellodextrin phosphorylase on insoluble cellulose. Appl Microbiol Biotechnol. 2011;92:551-60 pubmed publisher
    ..g., CBM3 (type A) from C. thermocellum CbhA, CBM4-2 (type B) from Rhodothermus marinus Xyn10A, CBM6 (type B) from Cellvibrio mixtus Cel5B, and CBM9-2 (type C) from Thermotoga maritima Xyn10A,..
  39. Fujiwara T, Saburi W, Matsui H, Mori H, Yao M. Structural insights into the epimerization of ?-1,4-linked oligosaccharides catalyzed by cellobiose 2-epimerase, the sole enzyme epimerizing non-anomeric hydroxyl groups of unmodified sugars. J Biol Chem. 2014;289:3405-15 pubmed publisher
    ..We determined the crystal structures of halothermophile Rhodothermus marinus CE (RmCE) in complex with substrates/products or intermediate analogs, and its apo form...
  40. Melo A, Lobo S, Sousa F, Fernandes A, Pereira M, Hreggvidsson G, et al. A nhaD Na+/H+ antiporter and a pcd homologues are among the Rhodothermus marinus complex I genes. Biochim Biophys Acta. 2005;1709:95-103 pubmed
    ..oxidoreductase (Nqo) is one of the enzymes present in the respiratory chain of the thermohalophilic bacterium Rhodothermus marinus. The genes coding for the R...
  41. Montilla A, Ruiz Matute A, Corzo N, Giacomini C, Irazoqui G. Enzymatic generation of chitooligosaccharides from chitosan using soluble and immobilized glycosyltransferase (Branchzyme). J Agric Food Chem. 2013;61:10360-7 pubmed publisher
    ..The reaction mixture was constituted by chitooligosaccharides with degrees of polymerization of between 2 and 20, with a higher concentration having degrees of polymerization of 3-8. ..
  42. Jorge C, Fonseca L, Boos W, Santos H. Role of periplasmic trehalase in uptake of trehalose by the thermophilic bacterium Rhodothermus marinus. J Bacteriol. 2008;190:1871-8 pubmed publisher
    Trehalose uptake at 65 degrees C in Rhodothermus marinus was characterized...
  43. Veríssimo A, Sousa F, Baptista A, Teixeira M, Pereira M. Thermodynamic redox behavior of the heme centers in A-type heme-copper oxygen reductases: comparison between the two subfamilies. Biophys J. 2008;95:4448-55 pubmed publisher
    ..two members of the A family of heme-copper oxygen reductases, Paracoccus denitrificans aa3 (A1 subfamily) and Rhodothermus marinus caa3 (A2 subfamily) enzymes, is presented...
  44. Sharma M, Luthra Guptasarma M. Degradation of proteins upon storage at near-neutral pH: indications of a proteolytic/gelatinolytic activity associated with aggregates. Biochim Biophys Acta. 2009;1790:1282-94 pubmed publisher
    ..However, although aggregation has been thought to be a possible consequence of protein degradation, it has never before been proposed to be a cause of degradation...
  45. Faria T, Knapp S, Ladenstein R, Maçanita A, Santos H. Protein stabilisation by compatible solutes: effect of mannosylglycerate on unfolding thermodynamics and activity of ribonuclease A. Chembiochem. 2003;4:734-41 pubmed
    ..The results are discussed in view of the physiological role of this charged compatible solute...
  46. Blondal T, Hjorleifsdottir S, Fridjonsson O, Aevarsson A, Skirnisdottir S, Hermannsdottir A, et al. Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1. Nucleic Acids Res. 2003;31:7247-54 pubmed
    ..a thermostable RNA ligase 1 from the thermophilic bacteriophage RM378 that infects the thermophilic eubacterium Rhodothermus marinus...
  47. Ramchuran S, Mateus B, Holst O, Karlsson E. The methylotrophic yeast Pichia pastoris as a host for the expression and production of thermostable xylanase from the bacterium Rhodothermus marinus. FEMS Yeast Res. 2005;5:839-50 pubmed
    A thermostable glycoside hydrolase family-10 xylanase originating from Rhodothermus marinus was cloned and expressed in the methylotrophic yeast Pichia pastoris (SMD1168H)...
  48. Wu Z, Dong H, Zou L, Lu D, Liu Z. Enriched microbial community in bioaugmentation of petroleum-contaminated soil in the presence of wheat straw. Appl Biochem Biotechnol. 2011;164:1071-82 pubmed publisher
    ..Seeding with E. cloacae stimulated the growth of other degrading stains such as Pseudomonas sp. and Rhodothermus sp...
  49. Batista A, Marreiros B, Louro R, Pereira M. Study of ion translocation by respiratory complex I. A new insight using (23)Na NMR spectroscopy. Biochim Biophys Acta. 2012;1817:1810-6 pubmed publisher
    ..This article is part of a Special Issue entitled: 17th European Bioenergetics Conference (EBEC 2012)...
  50. Neustroev K, Golubev A, Sinnott M, Borriss R, Krah M, Brumer H, et al. Transferase and hydrolytic activities of the laminarinase from Rhodothermus marinus and its M133A, M133C, and M133W mutants. Glycoconj J. 2006;23:501-11 pubmed
    Comparative studies of the transglycosylation and hydrolytic activities have been performed on the Rhodothermus marinus beta-1,3-glucanase (laminarinase) and its M133A, M133C, and M133W mutants...
  51. Kapoor D, Chandrayan S, Ahmed S, Guptasarma P. Using DNA sequencing electrophoresis compression artifacts as reporters of stable mRNA structures affecting gene expression. Electrophoresis. 2007;28:3862-7 pubmed
    ..Our study involves an engineered version of a gene sourced from Rhodothermus marinus encoding an enzyme called Cel12A...
  52. Falck P, Precha Atsawanan S, Grey C, Immerzeel P, Stålbrand H, Adlercreutz P, et al. Xylooligosaccharides from hardwood and cereal xylans produced by a thermostable xylanase as carbon sources for Lactobacillus brevis and Bifidobacterium adolescentis. J Agric Food Chem. 2013;61:7333-40 pubmed publisher
    ..were hydrolyzed using two variants of the xylanase RmXyn10A, full-length enzyme and catalytic module only, from Rhodothermus marinus ...
  53. Stelter M, Melo A, Hreggvidsson S, Saraiva L, Teixeira M, Archer M. Structure at 1.0 A resolution of a high-potential iron-sulfur protein involved in the aerobic respiratory chain of Rhodothermus marinus. J Biol Inorg Chem. 2010;15:303-13 pubmed publisher
    The aerobic respiratory chain of the thermohalophilic bacterium Rhodothermus marinus, a nonphotosynthetic organism from the Bacteroidetes/Chlorobi group, contains a high-potential iron-sulfur protein (HiPIP) that transfers electrons from ..
  54. Refojo P, Sousa F, Teixeira M, Pereira M. The alternative complex III: a different architecture using known building modules. Biochim Biophys Acta. 2010;1797:1869-76 pubmed publisher
    ..a complex with the same activity and with a unique subunit composition was purified from the membranes of Rhodothermus marinus...
  55. Fernandes A, Sousa F, Teixeira M, Pereira M. Electron paramagnetic resonance studies of the iron-sulfur centers from complex I of Rhodothermus marinus. Biochemistry. 2006;45:1002-8 pubmed
    b>Rhodothermus marinus, a thermohalophilic gram negative bacterium, contains a type I NADH/quinone oxidoreductase (complex I). Its purification was optimized, yielding large amounts of pure and active protein...
  56. Fernandes A, Konstantinov A, Teixeira M, Pereira M. Quinone reduction by Rhodothermus marinus succinate:menaquinone oxidoreductase is not stimulated by the membrane potential. Biochem Biophys Res Commun. 2005;330:565-70 pubmed
    Succinate:quinone oxidoreductase (SQR), a di-haem enzyme purified from Rhodothermus marinus, reveals an HQNO-sensitive succinate:quinone oxidoreductase activity with several menaquinone analogues as electron acceptors that decreases with ..
  57. Ruan L, Luo T, Li F, Xu X. Identification of differentially expressed genes from Rhodothermus sp. XMH10 in response to low temperature using random arbitrarily primed PCR. Curr Microbiol. 2007;55:543-8 pubmed
    ..In this report, the thermophilic bacterium Rhodothermus sp. XMH10 was examined to gain a better understanding of gene expression in response to low temperature...
  58. Batista A, Pereira M. Sodium influence on energy transduction by complexes I from Escherichia coli and Paracoccus denitrificans. Biochim Biophys Acta. 2011;1807:286-92 pubmed publisher
    ..has two energy coupling sites (one Na(+) independent and the other Na(+) dependent), as previously observed for Rhodothermus marinus complex I, whereas the coupling mechanism of P. denitrificans enzyme is completely Na(+) independent...
  59. Grimaud F, Lancelon Pin C, Rolland Sabaté A, Roussel X, Laguerre S, Viksø Nielsen A, et al. In vitro synthesis of hyperbranched ?-glucans using a biomimetic enzymatic toolbox. Biomacromolecules. 2013;14:438-47 pubmed publisher
    ..two bacterial transglucosidases, the amylosucrase from Neisseria polysaccharea and the branching enzyme from Rhodothermus obamensis ...
  60. Bjornsdottir S, Fridjonsson O, Hreggvidsson G, Eggertsson G. Generation of targeted deletions in the genome of Rhodothermus marinus. Appl Environ Microbiol. 2011;77:5505-12 pubmed publisher
    The aim of this work was to develop an approach for chromosomal engineering of the thermophile Rhodothermus marinus. A selection strategy for R...
  61. Sandoval C, BAKER S, Jansen K, Metzner S, Sousa M. Crystal structure of BamD: an essential component of the ?-Barrel assembly machinery of gram-negative bacteria. J Mol Biol. 2011;409:348-57 pubmed publisher
    ..Here, we present the crystal structure of BamD from the thermophilic bacteria Rhodothermus marinus refined to 2.15 Å resolution...
  62. Nielsen M, Suits M, Yang M, Barry C, Martinez Fleites C, Tailford L, et al. Substrate and metal ion promiscuity in mannosylglycerate synthase. J Biol Chem. 2011;286:15155-64 pubmed publisher
    ..Past work has shown that the Rhodothermus marinus MGS, classified as a GT78 glycosyltransferase, displays a GT-A fold and performs catalysis in a metal ..
  63. Huang Y, Krauss G, Cottaz S, Driguez H, Lipps G. A highly acid-stable and thermostable endo-beta-glucanase from the thermoacidophilic archaeon Sulfolobus solfataricus. Biochem J. 2005;385:581-8 pubmed
    ..A cellulolytic enzyme with such a combination of stability and activity at high temperatures and low pH has not been described so far and could be a valuable tool for the large-scale hydrolysis of cellulose under acidic conditions...
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    ..This cocktail will enable the development of novel biomass to biofuel bioprocessing configurations that may overcome some of the barriers to production of inexpensive cellulosic biofuels...
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    ..The substitution pattern of the derivatized cellulose polymers, i.e. the mode on which the substituent groups are dispersed along the cellulose backbone, can vary from batch-to-batch and is a factor that can influence drug release...
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    Cytochrome c from Rhodothermus marinus has been crystallized using the hanging-drop vapor-diffusion method in 30 % (w/v) polyethylene glycol 8K, 0.2 M ammonium sulfate, 8 % hexanediol and 50 mM sodium citrate pH 2.2...
  67. Sato H, Saburi W, Ojima T, Taguchi H, Mori H, Matsui H. Immobilization of a thermostable cellobiose 2-epimerase from Rhodothermus marinus JCM9785 and continuous production of epilactose. Biosci Biotechnol Biochem. 2012;76:1584-7 pubmed
    ..A thermostable CE from Rhodothermus marinus was immobilized on Duolite A568 and packed into a column...
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    In the thermohalophilic bacterium Rhodothermus marinus, the NADH:quinone oxidoreductase (complex I) is encoded by two single genes and two operons, one of which contains the genes for five complex I subunits, nqo10-nqo14, a pterin ..
  69. Ojima T, Saburi W, Sato H, Yamamoto T, Mori H, Matsui H. Biochemical characterization of a thermophilic cellobiose 2-epimerase from a thermohalophilic bacterium, Rhodothermus marinus JCM9785. Biosci Biotechnol Biochem. 2011;75:2162-8 pubmed
    ..We focused on thermoholophilic Rhodothermus marinus JCM9785 as a CE producer, since a CE-like gene was found in the genome of R. marinus DSM4252...
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    ..In this article, we report the identification of the subunits of complex I from Rhodothermus marinus...
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    ..This reaction occurs through the cleavage of ?(1?4) linkage and transfer in ?(1?6) of the fragment in non-reducing position. These enzymes define major elements that control the structure of both glycogen and starch...