thermococcus

Summary

Summary: A genus of extremely thermophilic heterotrophic archaea, in the family THERMOCOCCACEAE, occurring in heated sea flows. They are anaerobic chemoorganotropic sulfidogens.

Top Publications

  1. Soler N, Marguet E, Cortez D, Desnoues N, Keller J, van Tilbeurgh H, et al. Two novel families of plasmids from hyperthermophilic archaea encoding new families of replication proteins. Nucleic Acids Res. 2010;38:5088-104 pubmed publisher
    ..The plasmids pTN2 from Thermococcus nautilus sp. 30-1 and pP12-1 from Pyrococcus sp. 12-1 belong to the same family...
  2. Hopfner K, Eichinger A, Engh R, Laue F, Ankenbauer W, Huber R, et al. Crystal structure of a thermostable type B DNA polymerase from Thermococcus gorgonarius. Proc Natl Acad Sci U S A. 1999;96:3600-5 pubmed
    ..We describe here the 2.5 A resolution crystal structure of a DNA polymerase from the Archaea Thermococcus gorgonarius and identify structural features of the fold and the active site that are likely responsible for ..
  3. Lee C, Allen M, Bycroft M, Wong K. Electrostatic interactions contribute to reduced heat capacity change of unfolding in a thermophilic ribosomal protein l30e. J Mol Biol. 2005;348:419-31 pubmed publisher
    ..temperature dependency of the free energy change of unfolding, for a thermophilic ribosomal protein L30e from Thermococcus celer, and its mesophilic homologue from yeast...
  4. Ito S, Fushinobu S, Yoshioka I, Koga S, Matsuzawa H, Wakagi T. Structural basis for the ADP-specificity of a novel glucokinase from a hyperthermophilic archaeon. Structure. 2001;9:205-14 pubmed
    ATP is the most common phosphoryl group donor for kinases. However, certain hyperthermophilic archaea such as Thermococcus litoralis and Pyrococcus furiosus utilize unusual ADP-dependent glucokinases and phosphofructokinases in their ..
  5. Bálint B, Bagi Z, Toth A, RAKHELY G, Perei K, Kovacs K. Utilization of keratin-containing biowaste to produce biohydrogen. Appl Microbiol Biotechnol. 2005;69:404-10 pubmed
    ..step, in which the product of keratin fermentation--supplemented with essential minerals--was metabolized by Thermococcus litoralis, an anaerobic hyperthermophilic archaeon. T...
  6. Bashir Q, Rashid N, Jamil F, Imanaka T, Akhtar M. Highly thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Thermococcus kodakaraensis. J Biochem. 2009;146:95-102 pubmed publisher
    ..A search of the genome sequence of hyperthermophilic archaeon, Thermococcus kodakaraensis revealed the presence of a closely related orthologue (TK0916) of archaeal and bacterial l-..
  7. Tanaka T, Fukui T, Fujiwara S, Atomi H, Imanaka T. Concerted action of diacetylchitobiose deacetylase and exo-beta-D-glucosaminidase in a novel chitinolytic pathway in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. J Biol Chem. 2004;279:30021-7 pubmed publisher
    The hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 possesses chitinase (Tk-ChiA) and exo-beta-D-glucosaminidase (Tk-GlmA) for chitin degradation; the former produces diacetylchitobiose (GlcNAc2) from chitin, and the latter ..
  8. Yokooji Y, Tomita H, Atomi H, Imanaka T. Pantoate kinase and phosphopantothenate synthetase, two novel enzymes necessary for CoA biosynthesis in the Archaea. J Biol Chem. 2009;284:28137-45 pubmed publisher
    ..When we examined the cell-free extracts of the hyperthermophilic archaeon Thermococcus kodakaraensis, PanK activity could not be detected...
  9. Kuba Y, Ishino S, Yamagami T, Tokuhara M, Kanai T, Fujikane R, et al. Comparative analyses of the two proliferating cell nuclear antigens from the hyperthermophilic archaeon, Thermococcus kodakarensis. Genes Cells. 2012;17:923-37 pubmed publisher
    ..Among the Euryarchaeota whose genome is sequenced, Thermococcus kodakarensis is the only species with two genes encoding PCNA homologues on its genome...

More Information

Publications93

  1. Santangelo T, Cubonová L, Reeve J. Shuttle vector expression in Thermococcus kodakaraensis: contributions of cis elements to protein synthesis in a hyperthermophilic archaeon. Appl Environ Microbiol. 2008;74:3099-104 pubmed publisher
    Shuttle vectors that replicate stably and express selectable phenotypes in both Thermococcus kodakaraensis and Escherichia coli have been constructed. Plasmid pTN1 from Thermococcus nautilis was ligated to the commercial vector pCR2...
  2. Lee S, Engelmann A, Horlacher R, Qu Q, Vierke G, Hebbeln C, et al. TrmB, a sugar-specific transcriptional regulator of the trehalose/maltose ABC transporter from the hyperthermophilic archaeon Thermococcus litoralis. J Biol Chem. 2003;278:983-90 pubmed
    ..of 38,800 apparent molecular weight, that is involved in the maltose-specific regulation of a gene cluster in Thermococcus litoralis, malE malF malG orf trmB malK, encoding a binding protein-dependent ABC transporter for trehalose and ..
  3. Kempenaers M, Roovers M, Oudjama Y, Tkaczuk K, Bujnicki J, Droogmans L. New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA. Nucleic Acids Res. 2010;38:6533-43 pubmed publisher
    ..Even more surprisingly, the Trm10p orthologue from the euryarchaeon Thermococcus kodakaraensis methylates the N(1)-atom of either adenosine or guanosine at position 9 in different tRNAs...
  4. Pulido M, Tanaka S, Sringiew C, You D, Matsumura H, Koga Y, et al. Requirement of left-handed glycine residue for high stability of the Tk-subtilisin propeptide as revealed by mutational and crystallographic analyses. J Mol Biol. 2007;374:1359-73 pubmed publisher
    ..the mature domain of Pro-Tk-subtilisin in active form (Gly70-Gly398)] from the hyperthermophilic archaeon Thermococcus kodakaraensis is matured from Pro-Tk-subtilisin [a subtilisin homologue from T...
  5. Santangelo T, Cubonová L, Reeve J. Thermococcus kodakarensis genetics: TK1827-encoded beta-glycosidase, new positive-selection protocol, and targeted and repetitive deletion technology. Appl Environ Microbiol. 2010;76:1044-52 pubmed publisher
    Inactivation of TK1761, the reporter gene established for Thermococcus kodakarensis, revealed the presence of a second beta-glycosidase that we have identified as the product of TK1827...
  6. Zivanovic Y, Armengaud J, Lagorce A, Leplat C, Guerin P, Dutertre M, et al. Genome analysis and genome-wide proteomics of Thermococcus gammatolerans, the most radioresistant organism known amongst the Archaea. Genome Biol. 2009;10:R70 pubmed publisher
    b>Thermococcus gammatolerans was isolated from samples collected from hydrothermal chimneys. It is one of the most radioresistant organisms known amongst the Archaea...
  7. Shiraki K, Tsuji M, Hashimoto Y, Fujimoto K, Fujiwara S, Takagi M, et al. Genetic, enzymatic, and structural analyses of phenylalanyl-tRNA synthetase from Thermococcus kodakaraensis KOD1. J Biochem. 2003;134:567-74 pubmed
    Phenylalanyl-tRNA synthetase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 (Tk-PheRS) was cloned...
  8. Han T, Zeng F, Li Z, Liu L, Wei M, Guan Q, et al. Biochemical characterization of a recombinant pullulanase from Thermococcus kodakarensis KOD1. Lett Appl Microbiol. 2013;57:336-43 pubmed publisher
    ..a glycoside hydrolase 13 family pullulanase gene (Tk0977) was cloned from a thermophilic anaerobic archaeon Thermococcus kodakarensis KOD1 (Pul-Tk)...
  9. Morimoto N, Fukuda W, Nakajima N, Masuda T, Terui Y, Kanai T, et al. Dual biosynthesis pathway for longer-chain polyamines in the hyperthermophilic archaeon Thermococcus kodakarensis. J Bacteriol. 2010;192:4991-5001 pubmed publisher
    ..polyamines were analyzed for cells cultivated at various growth temperatures in the hyperthermophilic archaeon Thermococcus kodakarensis...
  10. Santangelo T, Cubonová L, Reeve J. Deletion of alternative pathways for reductant recycling in Thermococcus kodakarensis increases hydrogen production. Mol Microbiol. 2011;81:897-911 pubmed publisher
    Hydrogen (H?) production by Thermococcus kodakarensis compares very favourably with the levels reported for the most productive algal, fungal and bacterial systems. T...
  11. Sato T, Atomi H, Imanaka T. Archaeal type III RuBisCOs function in a pathway for AMP metabolism. Science. 2007;315:1003-6 pubmed publisher
    The type III ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) present in the archaeon Thermococcus kodakaraensis was found to participate in adenosine 5'-monophosphate (AMP) metabolism, a role that is distinct from that of ..
  12. Diederichs K, Diez J, Greller G, Muller C, Breed J, Schnell C, et al. Crystal structure of MalK, the ATPase subunit of the trehalose/maltose ABC transporter of the archaeon Thermococcus litoralis. EMBO J. 2000;19:5951-61 pubmed
    ..The structure and its relationship to function are discussed in the light of known mutations from the homologous Escherichia coli and Salmonella typhimurium proteins...
  13. Wong K, Lee C, Chan S, Leung T, Chen Y, Bycroft M. Solution structure and thermal stability of ribosomal protein L30e from hyperthermophilic archaeon Thermococcus celer. Protein Sci. 2003;12:1483-95 pubmed publisher
    ..of thermostability, we have determined the solution structure of a thermophilic ribosomal protein L30e from Thermococcus celer by NMR spectroscopy. The conformational stability of T...
  14. Keller J, Leulliot N, Soler N, Collinet B, Vincentelli R, Forterre P, et al. A protein encoded by a new family of mobile elements from Euryarchaea exhibits three domains with novel folds. Protein Sci. 2009;18:850-5 pubmed publisher
    ..which is encoded by an ORF of the plasmid pT26-2, recently isolated from the hyperthermophilic archaeon, Thermococcus sp. 26,2...
  15. Foophow T, Tanaka S, Koga Y, Takano K, Kanaya S. Subtilisin-like serine protease from hyperthermophilic archaeon Thermococcus kodakaraensis with N- and C-terminal propeptides. Protein Eng Des Sel. 2010;23:347-55 pubmed publisher
    The genome of the hyperthermophilic archaeon Thermococcus kodakaraensis contains three genes encoding subtilisin-like serine proteases, Tk-1689, Tk-0076 and Tk-subtilisin...
  16. Pan M, Santangelo T, Cubonová L, Li Z, Metangmo H, Ladner J, et al. Thermococcus kodakarensis has two functional PCNA homologs but only one is required for viability. Extremophiles. 2013;17:453-61 pubmed publisher
    ..Most eukarya and euryarchaea have only one PCNA homolog but Thermococcus kodakarensis uniquely has two, designated PCNA1 and PCNA2, encoded by TK0535 and TK0582, respectively...
  17. Tanaka S, Takeuchi Y, Matsumura H, Koga Y, Takano K, Kanaya S. Crystal structure of Tk-subtilisin folded without propeptide: requirement of propeptide for acceleration of folding. FEBS Lett. 2008;582:3875-8 pubmed publisher
    Tk-subtilisin (a subtilisin homologue from Thermococcus kodakaraensis) is matured from Pro-Tk-subtilisin upon autoprocessing and degradation of Tk-propeptide...
  18. Kanai T, Imanaka H, Nakajima A, Uwamori K, Omori Y, Fukui T, et al. Continuous hydrogen production by the hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1. J Biotechnol. 2005;116:271-82 pubmed
    The hydrogen (H2) production potential of the hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1 was evaluated at 85 degrees C...
  19. Matsumura H, Takahashi H, Inoue T, Yamamoto T, Hashimoto H, Nishioka M, et al. Crystal structure of intein homing endonuclease II encoded in DNA polymerase gene from hyperthermophilic archaeon Thermococcus kodakaraensis strain KOD1. Proteins. 2006;63:711-5 pubmed publisher
  20. Fukuda W, Fukui T, Atomi H, Imanaka T. First characterization of an archaeal GTP-dependent phosphoenolpyruvate carboxykinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. J Bacteriol. 2004;186:4620-7 pubmed publisher
    ..of the enzymatic properties and expression profile of an archaeal PCK from the hyperthermophilic archaeon Thermococcus kodakaraensis (PckTk)...
  21. Pulido M, Saito K, Tanaka S, Koga Y, Morikawa M, Takano K, et al. Ca2+-dependent maturation of subtilisin from a hyperthermophilic archaeon, Thermococcus kodakaraensis: the propeptide is a potent inhibitor of the mature domain but is not required for its folding. Appl Environ Microbiol. 2006;72:4154-62 pubmed
    Subtilisin from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 is a member of the subtilisin family. T. kodakaraensis subtilisin in a proform (T. kodakaraensis pro-subtilisin), as well as its propeptide (T...
  22. Mardanov A, Ravin N, Svetlitchnyi V, Beletsky A, Miroshnichenko M, Bonch Osmolovskaya E, et al. Metabolic versatility and indigenous origin of the archaeon Thermococcus sibiricus, isolated from a siberian oil reservoir, as revealed by genome analysis. Appl Environ Microbiol. 2009;75:4580-8 pubmed publisher
    b>Thermococcus species are widely distributed in terrestrial and marine hydrothermal areas, as well as in deep subsurface oil reservoirs...
  23. Lee M, Gonzalez J, Robb F. Extremely thermostable glutamate dehydrogenase (GDH) from the freshwater archaeon Thermococcus waiotapuensis: cloning and comparison with two marine hyperthermophilic GDHs. Extremophiles. 2002;6:151-9 pubmed
    ..A gene encoding GDH from the terrestrial hyperthermophilic archaeon Thermococcus waiotapuensis (Twaio) was cloned, sequenced, and expressed at a high level in Escherichia coli...
  24. Lepage E, Marguet E, Geslin C, Matte Tailliez O, Zillig W, Forterre P, et al. Molecular diversity of new Thermococcales isolates from a single area of hydrothermal deep-sea vents as revealed by randomly amplified polymorphic DNA fingerprinting and 16S rRNA gene sequence analysis. Appl Environ Microbiol. 2004;70:1277-86 pubmed
    ..One RAPD group corresponds to new isolates of Thermococcus hydrothermalis, whereas all other groups and isolates with unique profiles are different from the 22 reference ..
  25. Yoshida T, Yohda M, Iida T, Maruyama T, Taguchi H, Yazaki K, et al. Structural and functional characterization of homo-oligomeric complexes of alpha and beta chaperonin subunits from the hyperthermophilic archaeum Thermococcus strain KS-1. J Mol Biol. 1997;273:635-45 pubmed publisher
    ..To elucidate the function of group II chaperonin, the gene for the chaperonin from the hyperthermophilic archaeum Thermococcus strain KS-1 was cloned and sequenced...
  26. Mukaiyama A, Takano K, Haruki M, Morikawa M, Kanaya S. Kinetically robust monomeric protein from a hyperthermophile. Biochemistry. 2004;43:13859-66 pubmed
    ..the energetic features of the high stability of a monomeric protein, ribonuclease HII, from a hyperthermophile, Thermococcus kodakaraensis (Tk-RNase HII)...
  27. Takemasa R, Yokooji Y, Yamatsu A, Atomi H, Imanaka T. Thermococcus kodakarensis as a host for gene expression and protein secretion. Appl Environ Microbiol. 2011;77:2392-8 pubmed publisher
    Taking advantage of the gene manipulation system developed in Thermococcus kodakarensis, here, we developed a system for gene expression and efficient protein secretion using this hyperthermophilic archaeon as a host cell...
  28. Awano T, Wilming A, Tomita H, Yokooji Y, Fukui T, Imanaka T, et al. Characterization of two members among the five ADP-forming acyl coenzyme A (Acyl-CoA) synthetases reveals the presence of a 2-(Imidazol-4-yl)acetyl-CoA synthetase in Thermococcus kodakarensis. J Bacteriol. 2014;196:140-7 pubmed publisher
    The genome of Thermococcus kodakarensis, along with those of most Thermococcus and Pyrococcus species, harbors five paralogous genes encoding putative ? subunits of nucleoside diphosphate (NDP)-forming acyl coenzyme A (acyl-CoA) ..
  29. Littlechild J. Thermophilic archaeal enzymes and applications in biocatalysis. Biochem Soc Trans. 2011;39:155-8 pubmed publisher
    ..archaea contain enzymes that have already been used in commercial applications such as the L-aminoacylase from Thermococcus litoralis for the resolution of amino acids and amino acid analogues...
  30. Oku T, Ishikawa K. Analysis of the hyperthermophilic chitinase from Pyrococcus furiosus: activity toward crystalline chitin. Biosci Biotechnol Biochem. 2006;70:1696-701 pubmed
    ..Pyrococcus furiosus, we found two adjacent genes (PF1233 and PF1234) homologous to those of the chitinase of Thermococcus kodakaraensis. In the cultured medium of P. furiosus, however, no chitinase activity was detected...
  31. Oyama T, Ishino S, Fujino S, Ogino H, Shirai T, Mayanagi K, et al. Architectures of archaeal GINS complexes, essential DNA replication initiation factors. BMC Biol. 2011;9:28 pubmed publisher
    ..Due to the low sequence similarity between the archaeal and eukaryotic GINS subunits, the atomic structures of the archaeal GINS complexes are attracting interest for comparisons of their subunit architectures and organization...
  32. Chemnitz Galal W, Pan M, Giulian G, Yuan W, Li S, Edwards J, et al. Formation of dAMP-glycerol and dAMP-Tris derivatives by Thermococcus kodakaraensis DNA primase. J Biol Chem. 2012;287:16220-9 pubmed publisher
    In the presence of dATP, glycerol, and Tris buffer, the DNA primase isolated from Thermococcus kodakaraensis catalyzed the formation of dAMP and two products that were identified as dAMP-glycerol and dAMP-Tris...
  33. Yoshida T, Kawaguchi R, Taguchi H, Yoshida M, Yasunaga T, Wakabayashi T, et al. Archaeal group II chaperonin mediates protein folding in the cis-cavity without a detachable GroES-like co-chaperonin. J Mol Biol. 2002;315:73-85 pubmed
    ..alpha or beta-subunit chaperonin homo-oligomer (alpha16mer and beta16mer) from a hyperthermoplilic archaeum, Thermococcus strain KS-1, using a model substrate, green fluorescent protein (GFP)...
  34. Jia B, Lee S, Pham B, Kwack J, Jin H, Li J, et al. Biochemical characterization of deblocking aminopeptidases from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1. Biosci Biotechnol Biochem. 2011;75:1160-6 pubmed publisher
    ..Three DAP homologous (TkDAP1, TkDAP2, and TkDAP3) are annotated in the genome data base of Thermococcus kodakarensis KOD1...
  35. Chemnitz Galal W, Pan M, Kelman Z, Hurwitz J. Characterization of DNA primase complex isolated from the archaeon, Thermococcus kodakaraensis. J Biol Chem. 2012;287:16209-19 pubmed publisher
    ..the isolation and biochemical characterization of the DNA primase complex and its subunits from the archaeon Thermococcus kodakaraensis. The T...
  36. Cho S, Sun Y, Yu M, Kwon S, Kwon S. Characterization and PCR applications of dUTPase from the hyperthermophilic euryarchaeon Thermococcus pacificus. Enzyme Microb Technol. 2012;51:342-7 pubmed publisher
    We cloned and sequenced the gene encoding Thermococcus pacificus dUTPase (Tpa dUTPase). The Tpa dUTPase gene consists of 471 bp and encodes a 156-amino acid protein...
  37. Soler N, Justome A, Quevillon Cheruel S, Lorieux F, Le Cam E, Marguet E, et al. The rolling-circle plasmid pTN1 from the hyperthermophilic archaeon Thermococcus nautilus. Mol Microbiol. 2007;66:357-70 pubmed publisher
    The hyperthermophilic archaeon Thermococcus nautilus carries a plasmid, pTN1, which encodes a rolling-circle (RC) replication initiator protein of 74 kDa (Rep74) and an orphan protein of 24 kDa (p24)...
  38. Cho Y, Lee H, Kim Y, Kang S, Kim S, Lee J. Characterization of a dUTPase from the hyperthermophilic archaeon Thermococcus onnurineus NA1 and its application in polymerase chain reaction amplification. Mar Biotechnol (NY). 2007;9:450-8 pubmed
    Genomic analysis of the hyperthermophilic archaeon Thermococcus onnurineus NA1 (TNA1) revealed the presence of a 471-bp open reading frame with 93% similarity to the dUTPase from Pyrococcus furiosus...
  39. Rashid N, Kanai T, Atomi H, Imanaka T. Among multiple phosphomannomutase gene orthologues, only one gene encodes a protein with phosphoglucomutase and phosphomannomutase activities in Thermococcus kodakaraensis. J Bacteriol. 2004;186:6070-6 pubmed publisher
    ..phosphomannomutase (PMM) genes (COG1109) have been identified in the genome of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. We previously found that TK1777 actually encodes a phosphopentomutase...
  40. Sakuraba H, Kawakami R, Takahashi H, Ohshima T. Novel archaeal alanine:glyoxylate aminotransferase from Thermococcus litoralis. J Bacteriol. 2004;186:5513-8 pubmed
    A novel alanine:glyoxylate aminotransferase was found in a hyperthermophilic archaeon, Thermococcus litoralis. The amino acid sequence of the enzyme did not show a similarity to any alanine:glyoxylate aminotransferases reported so far...
  41. Lee J, Kim I, Jung J, Seo D, Kang S, Holden J, et al. Molecular cloning and enzymatic characterization of cyclomaltodextrinase from hyperthermophilic archaeon Thermococcus sp. CL1. J Microbiol Biotechnol. 2013;23:1060-9 pubmed
    ..near cyclomaltodextrinases (CDases) was analyzed and compared for four different hyperthermophilic archaea: Thermococcus, Pyrococcus, Staphylothermus, and Thermofilum. A gene (CL1_0884) encoding a putative CDase from Thermococcus sp...
  42. Akiba T, Ishii N, Rashid N, Morikawa M, Imanaka T, Harata K. Structure of RadB recombinase from a hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1: an implication for the formation of a near-7-fold helical assembly. Nucleic Acids Res. 2005;33:3412-23 pubmed publisher
    The X-ray crystal structure of RadB from Thermococcus kodakaraensis KOD1, an archaeal homologue of the RecA/Rad51 family proteins, have been determined in two crystal forms...
  43. Takano K, Endo S, Mukaiyama A, Chon H, Matsumura H, Koga Y, et al. Structure of amyloid beta fragments in aqueous environments. FEBS J. 2006;273:150-8 pubmed publisher
    ..28-42 (Abeta(28-42)), to three positions in the C-terminal region of ribonuclease HII from a hyperthermophile, Thermococcus kodakaraensis (Tk-RNase HII). We then examined the structural properties in an aqueous environment...
  44. Bae H, Kim K, Lee J, Song J, Kil E, Kim J, et al. Characterization of DNA polymerase from the hyperthermophilic archaeon Thermococcus marinus and its application to PCR. Extremophiles. 2009;13:657-67 pubmed publisher
    The family B DNA polymerase gene from the archaeon Thermococcus marinus (Tma) contains a long open reading frame of 3,939 bp that encodes 1,312 amino acid residues...
  45. Ying X, Ma K. Characterization of a zinc-containing alcohol dehydrogenase with stereoselectivity from the hyperthermophilic archaeon Thermococcus guaymasensis. J Bacteriol. 2011;193:3009-19 pubmed publisher
    An alcohol dehydrogenase (ADH) from hyperthermophilic archaeon Thermococcus guaymasensis was purified to homogeneity and was found to be a homotetramer with a subunit size of 40 ± 1 kDa...
  46. Takacs M, Toth A, Bogos B, Varga A, RAKHELY G, Kovacs K. Formate hydrogenlyase in the hyperthermophilic archaeon, Thermococcus litoralis. BMC Microbiol. 2008;8:88 pubmed publisher
    b>Thermococcus litoralis is a heterotrophic facultative sulfur dependent hyperthermophilic Archaeon, which was isolated from a shallow submarine thermal spring...
  47. Kim Y, Ryu Y, Lee H, Cho Y, Kwon S, Lee J, et al. Characterization of a dITPase from the hyperthermophilic archaeon Thermococcus onnurineus NA1 and its application in PCR amplification. Appl Microbiol Biotechnol. 2008;79:571-8 pubmed publisher
    ..To overcome the inhibitory effect of dITP during PCR amplification, a dITP pyrophosphatase (dITPase) from Thermococcus onnurineus NA1 was applied to PCR amplification. Genomic analysis of the hyperthermophilic archaeon T...
  48. Muroya A, Tsuchiya D, Ishikawa M, Haruki M, Morikawa M, Kanaya S, et al. Catalytic center of an archaeal type 2 ribonuclease H as revealed by X-ray crystallographic and mutational analyses. Protein Sci. 2001;10:707-14 pubmed publisher
    ..The crystal structure of the Type 2 RNase H from Thermococcus kodakaraensis KOD1 has revealed that the N-terminal major domain adopts the RNase H fold, despite the poor ..
  49. Chen Y, Bycroft M, Wong K. Crystal structure of ribosomal protein L30e from the extreme thermophile Thermococcus celer: thermal stability and RNA binding. Biochemistry. 2003;42:2857-65 pubmed publisher
    ..here the high-resolution crystal structure of the ribosomal protein L30e from the hyperthermophilic archaeon Thermococcus celer determined at cryo-temperature...
  50. Iizuka R, Yoshida T, Shomura Y, Miki K, Maruyama T, Odaka M, et al. ATP binding is critical for the conformational change from an open to closed state in archaeal group II chaperonin. J Biol Chem. 2003;278:44959-65 pubmed
    ..analyses and tryptophan fluorescence spectroscopy of alpha-chaperonin from a hyperthermophilic archaeum, Thermococcus strain KS-1...
  51. Bezsudnova E, Boyko K, Polyakov K, Dorovatovskiy P, Stekhanova T, Gumerov V, et al. Structural insight into the molecular basis of polyextremophilicity of short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Thermococcus sibiricus. Biochimie. 2012;94:2628-38 pubmed publisher
    Biochemical analysis of enantioselective short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Thermococcus sibiricus (TsAdh319) revealed unique polyextremophilic properties of the enzyme - half-life of 1 h at 100 °C, ..
  52. Maruyama H, Shin M, Oda T, Matsumi R, Ohniwa R, Itoh T, et al. Histone and TK0471/TrmBL2 form a novel heterogeneous genome architecture in the hyperthermophilic archaeon Thermococcus kodakarensis. Mol Biol Cell. 2011;22:386-98 pubmed publisher
    ..2 (TrmBL2), in addition to histone, is a novel type of abundant chromosomal protein in the model euryarchaeon Thermococcus kodakarensis . The chromosome of T...
  53. Lim J, Kang S, Lebedinsky A, Lee J, Lee H. Identification of a novel class of membrane-bound [NiFe]-hydrogenases in Thermococcus onnurineus NA1 by in silico analysis. Appl Environ Microbiol. 2010;76:6286-9 pubmed publisher
    In silico analysis of group 4 [NiFe]-hydrogenases from a hyperthermophilic archaeon, Thermococcus onnurineus NA1, revealed a novel tripartite gene cluster consisting of dehydrogenase-hydrogenase-cation/proton antiporter subunits, which ..
  54. Elmore J, Yokooji Y, Sato T, Olson S, Glover C, Graveley B, et al. Programmable plasmid interference by the CRISPR-Cas system in Thermococcus kodakarensis. RNA Biol. 2013;10:828-40 pubmed publisher
    ..There are multiple CRISPR-Cas systems with distinct compositions and mechanistic processes. Thermococcus kodakarensis (Tko) is a hyperthermophilic euryarchaeon that has both a Type I-A Csa and a Type I-B Cst CRISPR-..
  55. Kanai T, Akerboom J, Takedomi S, van de Werken H, Blombach F, van der Oost J, et al. A global transcriptional regulator in Thermococcus kodakaraensis controls the expression levels of both glycolytic and gluconeogenic enzyme-encoding genes. J Biol Chem. 2007;282:33659-70 pubmed
    ..Tgr), functioning as both an activator and a repressor of transcription in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1...
  56. Pikuta E, Marsic D, Itoh T, Bej A, Tang J, Whitman W, et al. Thermococcus thioreducens sp. nov., a novel hyperthermophilic, obligately sulfur-reducing archaeon from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol. 2007;57:1612-8 pubmed
    ..9 mol%. The 16S rRNA gene sequence analysis revealed that strain OGL-20P(T) is closely related to Thermococcus coalescens and related species, but no significant homology by DNA-DNA hybridization was observed between those ..
  57. Louvel H, Kanai T, Atomi H, Reeve J. The Fur iron regulator-like protein is cryptic in the hyperthermophilic archaeon Thermococcus kodakaraensis. FEMS Microbiol Lett. 2009;295:117-28 pubmed
    ..In this report, iron acquisition mechanisms were investigated in the hyperthermophilic archaeon Thermococcus kodakaraensis...
  58. Ogata N, Miura T. Elongation of tandem repetitive DNA by the DNA polymerase of the hyperthermophilic archaeon Thermococcus litoralis at a hairpin-coil transitional state: a model of amplification of a primordial simple DNA sequence. Biochemistry. 2000;39:13993-4001 pubmed
    ..in vitro to more than 20,000 bases at 70-74 degrees C by the DNA polymerase of the hyperthermophilic archaeon Thermococcus litoralis without a bimolecular primer-template complex...
  59. Imanaka H, Yamatsu A, Fukui T, Atomi H, Imanaka T. Phosphoenolpyruvate synthase plays an essential role for glycolysis in the modified Embden-Meyerhof pathway in Thermococcus kodakarensis. Mol Microbiol. 2006;61:898-909 pubmed publisher
    ..analysis on pyruvate kinase (PykTk) and phosphoenolpyruvate synthase (PpsTk) in the hyperthermophilic archaeon, Thermococcus kodakarensis...
  60. Yamamoto T, Matsuda T, Sakamoto N, Matsumura H, Inoue T, Morikawa M, et al. Crystallization and preliminary X-ray analysis of TBP-interacting protein from the hyperthermophilic archaeon Thermococcus kodakaraensis strain KOD1. Acta Crystallogr D Biol Crystallogr. 2003;59:372-4 pubmed
    The 26 kDa TBP (TATA-binding protein) interacting protein from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 (Tk-TIP26) is a possible transcriptional regulatory protein in Thermococcales...
  61. Kawakami R, Sakuraba H, Ohshima T. Gene and primary structures of dye-linked L-proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex. Extremophiles. 2004;8:99-108 pubmed
    ..The enzyme from the hyperthermophilic archaeon Thermococcus profundus was purified and characterized for the first time in archaea by Sakuraba et al. in 2001...
  62. Gorlas A, Koonin E, Bienvenu N, Prieur D, Geslin C. TPV1, the first virus isolated from the hyperthermophilic genus Thermococcus. Environ Microbiol. 2012;14:503-16 pubmed publisher
    We describe a novel virus, TPV1 (Thermococcus prieurii virus 1), which was discovered in a hyperthermophilic euryarchaeote isolated from a deep-sea hydrothermal chimney sample collected at a depth of 2700?m at the East Pacific Rise...
  63. Iizuka R, So S, Inobe T, Yoshida T, Zako T, Kuwajima K, et al. Role of the helical protrusion in the conformational change and molecular chaperone activity of the archaeal group II chaperonin. J Biol Chem. 2004;279:18834-9 pubmed
    ..its molecular chaperone function, three deletion mutants of the chaperonin from a hyperthermophilic archaeum (Thermococcus sp. strain KS-1) lacking one-third, two-thirds, and the whole of the helical protrusion were constructed...
  64. Tanaka S, Saito K, Chon H, Matsumura H, Koga Y, Takano K, et al. Crystal structure of unautoprocessed precursor of subtilisin from a hyperthermophilic archaeon: evidence for Ca2+-induced folding. J Biol Chem. 2007;282:8246-55 pubmed publisher
    ..crystal structure of an active site mutant of pro-Tk-subtilisin (pro-S324A) from the hyperthermophilic archaeon Thermococcus kodakaraensis was determined at 2.3 A resolution...
  65. Sato T, Fukui T, Atomi H, Imanaka T. Targeted gene disruption by homologous recombination in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. J Bacteriol. 2003;185:210-20 pubmed
    ..This study aimed to develop a gene disruption system for the hyperthermophilic euryarchaeon Thermococcus kodakaraensis KOD1...
  66. Fukuda W, Ismail Y, Fukui T, Atomi H, Imanaka T. Characterization of an archaeal malic enzyme from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. Archaea. 2005;1:293-301 pubmed
    ..uncharacterized homolog of malic enzyme identified in the genome of a heterotrophic, hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 (Tk-Mae)...
  67. Marguet E, Gaudin M, Gauliard E, Fourquaux I, le Blond du Plouy S, Matsui I, et al. Membrane vesicles, nanopods and/or nanotubes produced by hyperthermophilic archaea of the genus Thermococcus. Biochem Soc Trans. 2013;41:436-42 pubmed publisher
    b>Thermococcus species produce MVs (membrane vesicles) into their culture medium. These MVs are formed by a budding process from the cell envelope, similar to ectosome formation in eukaryotic cells...
  68. Pulido M, Koga Y, Takano K, Kanaya S. Directed evolution of Tk-subtilisin from a hyperthermophilic archaeon: identification of a single amino acid substitution responsible for low-temperature adaptation. Protein Eng Des Sel. 2007;20:143-53 pubmed
    Tk-subtilisin from the hyperthermophilic archaeon Thermococcus kodakaraensis is synthesized in a prepro-form (prepro-Tk-subtilisin), secreted in a pro-form (pro-Tk-subtilisin), and matured to an active form (mat-Tk-subtilisin*; a Ca(2+)-..
  69. Prieur D, Erauso G, Geslin C, Lucas S, Gaillard M, Bidault A, et al. Genetic elements of Thermococcales. Biochem Soc Trans. 2004;32:184-7 pubmed
    ..We also review the work accomplished over the last 5 years in the development of genetic tools for members of the Pyrococcus and Thermococcus genera, mainly in our laboratories.
  70. Tori K, Cheriyan M, Pedamallu C, Contreras M, Perler F. The Thermococcus kodakaraensis Tko CDC21-1 intein activates its N-terminal splice junction in the absence of a conserved histidine by a compensatory mechanism. Biochemistry. 2012;51:2496-505 pubmed publisher
    ..This His is present in all inteins identified to date except the Thermococcus kodakaraensis Tko CDC21-1 intein orthologs and the inactive Arthrobacter species FB24 Arth_1007 intein...
  71. De Stefano L, Vitale A, Rea I, Staiano M, Rotiroti L, Labella T, et al. Enzymes and proteins from extremophiles as hyperstable probes in nanotechnology: the use of D-trehalose/D-maltose-binding protein from the hyperthermophilic archaeon Thermococcus litoralis for sugars monitoring. Extremophiles. 2008;12:69-73 pubmed
    ..In the hyperthermophilic archaeon Thermococcus litoralis, this is mediated by a protein-dependent ATP-binding cassette system transporter...
  72. Wu X, Zhang C, Orita I, Imanaka T, Fukui T, Xing X. Thermostable alcohol dehydrogenase from Thermococcus kodakarensis KOD1 for enantioselective bioconversion of aromatic secondary alcohols. Appl Environ Microbiol. 2013;79:2209-17 pubmed publisher
    ..ADH) showing activity toward aromatic secondary alcohols was identified from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1 (TkADH)...
  73. Strand K, Sun C, Li T, Jenney F, Schut G, Adams M. Oxidative stress protection and the repair response to hydrogen peroxide in the hyperthermophilic archaeon Pyrococcus furiosus and in related species. Arch Microbiol. 2010;192:447-59 pubmed publisher
    ..Related hyperthermophiles contain homologs of the proteins involved in the constitutive protective mechanism but these organisms were more sensitive to peroxide than P. furiosus and lack several of its peroxide-responsive ORFs...
  74. Lee H, Kang S, Bae S, Lim J, Cho Y, Kim Y, et al. The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolism. J Bacteriol. 2008;190:7491-9 pubmed publisher
    Members of the genus Thermococcus, sulfur-reducing hyperthermophilic archaea, are ubiquitously present in various deep-sea hydrothermal vent systems and are considered to play a significant role in the microbial consortia...
  75. Shomura Y, Yoshida T, Iizuka R, Maruyama T, Yohda M, Miki K. Crystal structures of the group II chaperonin from Thermococcus strain KS-1: steric hindrance by the substituted amino acid, and inter-subunit rearrangement between two crystal forms. J Mol Biol. 2004;335:1265-78 pubmed
    ..of the alpha subunit with amino acid substitutions of G65C and/or I125T from the hyperthermophilic archaeum Thermococcus strain KS-1 were determined...
  76. Sato T, Fukui T, Atomi H, Imanaka T. Improved and versatile transformation system allowing multiple genetic manipulations of the hyperthermophilic archaeon Thermococcus kodakaraensis. Appl Environ Microbiol. 2005;71:3889-99 pubmed
    We have recently developed a gene disruption system for the hyperthermophilic archaeon Thermococcus kodakaraensis by utilizing a pyrF-deficient mutant, KU25, as a host strain and the pyrF gene as a selectable marker...
  77. Atomi H, Fukui T, Kanai T, Morikawa M, Imanaka T. Description of Thermococcus kodakaraensis sp. nov., a well studied hyperthermophilic archaeon previously reported as Pyrococcus sp. KOD1. Archaea. 2004;1:263-7 pubmed
    ..sequences of various species in the order Thermococcales, indicated that strain KOD1 is a member of the genus Thermococcus. We performed DNA-DNA hybridization tests against species that displayed high similarity in terms of 16S ..
  78. Hirata A, Kanai T, Santangelo T, Tajiri M, Manabe K, Reeve J, et al. Archaeal RNA polymerase subunits E and F are not required for transcription in vitro, but a Thermococcus kodakarensis mutant lacking subunit F is temperature-sensitive. Mol Microbiol. 2008;70:623-33 pubmed publisher
    ..By gene replacement, we have isolated archaeal mutants of Thermococcus kodakarensis with the subunit F-encoding gene (rpoF) deleted, but we were unable to isolate mutants lacking the ..
  79. Jeon S, Fujiwara S, Takagi M, Tanaka T, Imanaka T. Tk-PTP, protein tyrosine/serine phosphatase from hyperthermophilic archaeon Thermococcus kodakaraensis KOD1: enzymatic characteristics and identification of its substrate proteins. Biochem Biophys Res Commun. 2002;295:508-14 pubmed
    The Tk-ptp gene encoding a protein tyrosine phosphatase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 was cloned and biochemical characteristics of the recombinant protein (Tk-PTP) were examined...
  80. Krupovic M, Gonnet M, Hania W, Forterre P, Erauso G. Insights into dynamics of mobile genetic elements in hyperthermophilic environments from five new Thermococcus plasmids. PLoS ONE. 2013;8:e49044 pubmed publisher
    ..dynamics of mobile genetic elements in these environments we have sequenced five new plasmids from different Thermococcus strains that have been isolated from geographically remote hydrothermal vents...
  81. Raina M, Elgamal S, Santangelo T, Ibba M. Association of a multi-synthetase complex with translating ribosomes in the archaeon Thermococcus kodakarensis. FEBS Lett. 2012;586:2232-8 pubmed publisher
    ..MSC function was investigated in vivo in the archaeon Thermococcus kodakarensis, wherein six aaRSs were affinity co-purified together with several other factors involved in ..
  82. Park S, Pham B, Van Duyet L, Jia B, Lee S, Yu R, et al. Structural and functional characterization of osmotically inducible protein C (OsmC) from Thermococcus kodakaraensis KOD1. Biochim Biophys Acta. 2008;1784:783-8 pubmed publisher
    ..TkOsmC showed a homotetrameric structure based on gel filtration and electron microscopic analyses. TkOsmC has a significant peroxidase activity toward both organic and inorganic peroxides in high, but not in low temperature...
  83. Canganella F, Jones W, Gambacorta A, Antranikian G. Thermococcus guaymasensis sp. nov. and Thermococcus aggregans sp. nov., two novel thermophilic archaea isolated from the Guaymas Basin hydrothermal vent site. Int J Syst Bacteriol. 1998;48 Pt 4:1181-5 pubmed
    b>Thermococcus strains TYST and TYT isolated from the Guaymas Basin hydrothermal vent site and previously described were compared by DNA-DNA hybridization analysis with the closest Thermococcus species in terms of physiology and nutritional ..
  84. Matsumi R, Manabe K, Fukui T, Atomi H, Imanaka T. Disruption of a sugar transporter gene cluster in a hyperthermophilic archaeon using a host-marker system based on antibiotic resistance. J Bacteriol. 2007;189:2683-91 pubmed
    We have developed a gene disruption system in the hyperthermophilic archaeon Thermococcus kodakaraensis using the antibiotic simvastatin and a fusion gene designed to overexpress the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) ..