pyrococcus

Summary

Summary: A genus of strictly anaerobic ultrathermophilic archaea, in the family THERMOCOCCACEAE, occurring in heated seawaters. They exhibit heterotrophic growth at an optimum temperature of 100 degrees C.

Top Publications

  1. Marsin S, Forterre P. The active site of the rolling circle replication protein Rep75 is involved in site-specific nuclease, ligase and nucleotidyl transferase activities. Mol Microbiol. 1999;33:537-45 pubmed
    The plasmid pGT5 from the hyperthermophilic archaeon Pyrococcus abyssi replicates via a rolling circle mechanism...
  2. Sokabe M, Kawamura T, Sakai N, Yao M, Watanabe N, Tanaka I. The X-ray crystal structure of pyrrolidone-carboxylate peptidase from hyperthermophilic archaea Pyrococcus horikoshii. J Struct Funct Genomics. 2002;2:145-54 pubmed
    The crystal structure of pyrrolidone-carboxylate peptidase (PCP) from hyperthermophilic archaea Pyrococcus horikoshii (PhoPCP) has been determined at 1.6-A resolution by X-ray crystallography...
  3. Porcelli M, Moretti M, Concilio L, Forte S, Merlino A, Graziano G, et al. S-adenosylhomocysteine hydrolase from the archaeon Pyrococcus furiosus: biochemical characterization and analysis of protein structure by comparative molecular modeling. Proteins. 2005;58:815-25 pubmed publisher
    ..AdoHcyHD from the hyperthermophilic archaeon Pyrococcus furiosus (PfAdoHcyHD) was cloned, expressed in Escherichia coli, and purified...
  4. Ghosh M, Grunden A, Dunn D, Weiss R, Adams M. Characterization of native and recombinant forms of an unusual cobalt-dependent proline dipeptidase (prolidase) from the hyperthermophilic archaeon Pyrococcus furiosus. J Bacteriol. 1998;180:4781-9 pubmed
    Proline dipeptidase (prolidase) was purified from cell extracts of the proteolytic, hyperthermophilic archaeon Pyrococcus furiosus by multistep chromatography. The enzyme is a homodimer (39...
  5. Gerard E, Labedan B, Forterre P. Isolation of a minD-like gene in the hyperthermophilic archaeon Pyrococcus AL585, and phylogenetic characterization of related proteins in the three domains of life. Gene. 1998;222:99-106 pubmed
    The region upstream of the dinF-like gene of the hyperthermophilic archaeon Pyrococcus strain AL585 has been cloned and sequenced...
  6. Cohen G, Barbe V, Flament D, Galperin M, Heilig R, Lecompte O, et al. An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi. Mol Microbiol. 2003;47:1495-512 pubmed
    The hyperthermophilic euryarchaeon Pyrococcus abyssi and the related species Pyrococcus furiosus and Pyrococcus horikoshii, whose genomes have been completely sequenced, are presently used as model organisms in different laboratories to ..
  7. Fukui T, Atomi H, Kanai T, Matsumi R, Fujiwara S, Imanaka T. Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes. Genome Res. 2005;15:352-63 pubmed
    ..archaea, belongs to the order Thermococcales in Euryarchaeota along with the closely related genus Pyrococcus. The members of Thermococcus are ubiquitously present in natural high-temperature environments, and are ..
  8. Matsunaga F, Norais C, Forterre P, Myllykallio H. Identification of short 'eukaryotic' Okazaki fragments synthesized from a prokaryotic replication origin. EMBO Rep. 2003;4:154-8 pubmed
    ..archaeal genomes encode proteins similar to eukaryotic replication factors, the hyperthermophilic archaeon Pyrococcus abyssi replicates its circular chromosome at a high rate from a single origin (oriC) as in Bacteria...
  9. Gueguen Y, Rolland J, Lecompte O, Azam P, Le Romancer G, Flament D, et al. Characterization of two DNA polymerases from the hyperthermophilic euryarchaeon Pyrococcus abyssi. Eur J Biochem. 2001;268:5961-9 pubmed
    The complete genome sequence of the hyperthermophilic archaeon Pyrococcus abyssi revealed the presence of a family B DNA polymerase (Pol I) and a family D DNA polymerase (Pol II)...

More Information

Publications75

  1. Ward D, Revet I, Nandakumar R, Tuttle J, de Vos W, van der Oost J, et al. Characterization of plasmid pRT1 from Pyrococcus sp. strain JT1. J Bacteriol. 2002;184:2561-6 pubmed
    We discovered a 3,373-bp plasmid (pRT1) in the hyperthermophilic archaeon Pyrococcus sp. strain JT1...
  2. Thore S, Mayer C, Sauter C, Weeks S, Suck D. Crystal structures of the Pyrococcus abyssi Sm core and its complex with RNA. Common features of RNA binding in archaea and eukarya. J Biol Chem. 2003;278:1239-47 pubmed publisher
    ..Their proposed function is to mediate RNA-RNA interactions. We present here the crystal structures of Pyrococcus abyssi Sm protein (PA-Sm1) and its complex with a uridine heptamer...
  3. Muller S, Leclerc F, Behm Ansmant I, Fourmann J, Charpentier B, Branlant C. Combined in silico and experimental identification of the Pyrococcus abyssi H/ACA sRNAs and their target sites in ribosomal RNAs. Nucleic Acids Res. 2008;36:2459-75 pubmed publisher
    ..Hence here, by computational search in three Pyrococcus genomes, we identified seven H/ACA sRNAs and predicted their target sites in rRNAs...
  4. Dabrowski S, Kur J. Cloning and expression in Escherichia coli of the recombinant his-tagged DNA polymerases from Pyrococcus furiosus and Pyrococcus woesei. Protein Expr Purif. 1998;14:131-8 pubmed publisher
    Complete PCR-derived DNA fragments containing the structural genes for DNA polymerases of the archaeons Pyrococcus furiosus and Pyrococcus woesei were cloned into an expression vector...
  5. Van Boxstael S, Cunin R, Khan S, Maes D. Aspartate transcarbamylase from the hyperthermophilic archaeon Pyrococcus abyssi: thermostability and 1.8A resolution crystal structure of the catalytic subunit complexed with the bisubstrate analogue N-phosphonacetyl-L-aspartate. J Mol Biol. 2003;326:203-16 pubmed
    The Pyrococcus abyssi aspartate transcarbamylase (ATCase) shows a high degree of structural conservation with respect to the well-studied mesophilic Escherichia coli ATCase, including the association of catalytic and regulatory subunits...
  6. Geslin C, Le Romancer M, Erauso G, Gaillard M, Perrot G, Prieur D. PAV1, the first virus-like particle isolated from a hyperthermophilic euryarchaeote, "Pyrococcus abyssi". J Bacteriol. 2003;185:3888-94 pubmed
    ..the first virus-like particle of a hyperthermophilic euryarchaeote which was discovered in a strain of "Pyrococcus abyssi" previously characterized in our laboratory...
  7. Yokoyama K, Ishijima S, Koike H, Kurihara C, Shimowasa A, Kabasawa M, et al. Feast/famine regulation by transcription factor FL11 for the survival of the hyperthermophilic archaeon Pyrococcus OT3. Structure. 2007;15:1542-54 pubmed publisher
    Transcriptional repressor FL11 from the hyperthermophilic archaeon, Pyrococcus OT3, was crystallized in its dimer form in complex with a DNA duplex, TGAAAWWWTTTCA...
  8. Izumi M, Fujiwara S, Takagi M, Kanaya S, Imanaka T. Isolation and characterization of a second subunit of molecular chaperonin from Pyrococcus kodakaraensis KOD1: analysis of an ATPase-deficient mutant enzyme. Appl Environ Microbiol. 1999;65:1801-5 pubmed
    The cpkA gene encoding a second (alpha) subunit of archaeal chaperonin from Pyrococcus kodakaraensis KOD1 was cloned, sequenced, and expressed in Escherichia coli...
  9. Higashibata H, Fujiwara S, Takagi M, Imanaka T. Analysis of DNA compaction profile and intracellular contents of archaeal histones from Pyrococcus kodakaraensis KOD1. Biochem Biophys Res Commun. 1999;258:416-24 pubmed
    Two histone genes, hpkA and hpkB, from hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 strain were cloned, sequenced, and expressed in Escherichia coli cells...
  10. Villeret V, Clantin B, Tricot C, Legrain C, Roovers M, Stalon V, et al. The crystal structure of Pyrococcus furiosus ornithine carbamoyltransferase reveals a key role for oligomerization in enzyme stability at extremely high temperatures. Proc Natl Acad Sci U S A. 1998;95:2801-6 pubmed
    The Pyrococcus furiosus (PF) ornithine carbamoyltransferase (OTCase; EC 2.1.3.3) is an extremely heat-stable enzyme that maintains about 50% of its activity after heat treatment for 60 min at 100 degrees C...
  11. Maeda N, Kitano K, Fukui T, Ezaki S, Atomi H, Miki K, et al. Ribulose bisphosphate carboxylase/oxygenase from the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 is composed solely of large subunits and forms a pentagonal structure. J Mol Biol. 1999;293:57-66 pubmed publisher
    ..carboxylase-specific ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in a hyperthermophilic archaeon, Pyrococcus kodakaraensis KOD1. In this study, structural analysis of Pk -Rubisco has been performed...
  12. Lecompte O, Ripp R, Puzos Barbe V, Duprat S, Heilig R, Dietrich J, et al. Genome evolution at the genus level: comparison of three complete genomes of hyperthermophilic archaea. Genome Res. 2001;11:981-93 pubmed
    We have compared three complete genomes of closely related hyperthermophilic species of Archaea belonging to the Pyrococcus genus: Pyrococcus abyssi, Pyrococcus horikoshii, and Pyrococcus furiosus...
  13. Du X, Choi I, Kim R, Wang W, Jancarik J, Yokota H, et al. Crystal structure of an intracellular protease from Pyrococcus horikoshii at 2-A resolution. Proc Natl Acad Sci U S A. 2000;97:14079-84 pubmed publisher
    The intracellular protease from Pyrococcus horikoshii (PH1704) and PfpI from Pyrococcus furiosus are members of a class of intracellular proteases that have no sequence homology to any other known protease family...
  14. Gerard E, Jolivet E, Prieur D, Forterre P. DNA protection mechanisms are not involved in the radioresistance of the hyperthermophilic archaea Pyrococcus abyssi and P. furiosus. Mol Genet Genomics. 2001;266:72-8 pubmed
    Hyperthermophilic archaea of the genus Pyrococcus are resistant to gamma radiation, suggesting that efficient mechanisms for DNA repair exist in these organisms...
  15. Sakai N, Yao M, Itou H, Watanabe N, Yumoto F, Tanokura M, et al. The three-dimensional structure of septum site-determining protein MinD from Pyrococcus horikoshii OT3 in complex with Mg-ADP. Structure. 2001;9:817-26 pubmed
    ..The three-dimensional structure of the MinD-2 from Pyrococcus horikoshii OT3 (PH0612) has been determined at 2...
  16. Daabrowski S, Sobiewska G, Maciu ska J, Synowiecki J, Kur J. Cloning, expression, and purification of the His(6)-tagged thermostable beta-galactosidase from Pyrococcus woesei in Escherichia coli and some properties of the isolated enzyme. Protein Expr Purif. 2000;19:107-12 pubmed publisher
    In the previous study we cloned Pyrococcus woesei gene coding thermostable beta-galactosidase into pET30-LIC expression plasmid. The nucleotide sequence revealed that beta-galactosidase of P...
  17. Jeon S, Fujiwara S, Takagi M, Imanaka T. Pk-cdcA encodes a CDC48/VCP homolog in the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1: transcriptional and enzymatic characterization. Mol Gen Genet. 1999;262:559-67 pubmed
    A gene encoding a cell division control protein from the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1, Pk-cdcA, was cloned and sequenced...
  18. Nishioka M, Fujiwara S, Takagi M, Imanaka T. Characterization of two intein homing endonucleases encoded in the DNA polymerase gene of Pyrococcus kodakaraensis strain KOD1. Nucleic Acids Res. 1998;26:4409-12 pubmed
    ..endonucleases, denoted PI- Pko I and PI- Pko II, in the DNA polymerase gene of the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 were expressed in Escherichia coli and the recombinant endonucleases were characterized...
  19. Ren B, Tibbelin G, de Pascale D, Rossi M, Bartolucci S, Ladenstein R. A protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus contains two thioredoxin fold units. Nat Struct Biol. 1998;5:602-11 pubmed publisher
    ..resolution X-ray crystal structure of a protein disulfide oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus reveals structural details that suggest a relation to eukaryotic PDI...
  20. Xiong A, Peng R, Zhuang J, Li X, Xue Y, Liu J, et al. Directed evolution of a beta-galactosidase from Pyrococcus woesei resulting in increased thermostable beta-glucuronidase activity. Appl Microbiol Biotechnol. 2007;77:569-78 pubmed
    We performed directed evolution on a chemically synthesized 1,533-bp recombinant beta-galactosidase gene from Pyrococcus woesei...
  21. Kanoksilapatham W, Gonz lez J, Maeder D, DiRuggiero J, Robb F. A proposal to rename the hyperthermophile Pyrococcus woesei as Pyrococcus furiosus subsp. woesei. Archaea. 2004;1:277-83 pubmed
    b>Pyrococcus species are hyperthermophilic members of the order Thermococcales, with optimal growth temperatures approaching 100 degrees C...
  22. Constantinesco F, Benachenhou N, Motorin Y, Grosjean H. The tRNA(guanine-26,N2-N2) methyltransferase (Trm1) from the hyperthermophilic archaeon Pyrococcus furiosus: cloning, sequencing of the gene and its expression in Escherichia coli. Nucleic Acids Res. 1998;26:3753-61 pubmed
    ..1.1.32) of the strictly anaerobic hyperthermophilic archaeon Pyrococcus furiosus, has been identified by sequence similarity to the TRM1 gene of Saccharomyces cerevisiae (YDR120c)...
  23. Lepage E, Marguet E, Geslin C, Matte Tailliez O, Zillig W, Forterre P, et al. Molecular diversity of new Thermococcales isolates from a single area of hydrothermal deep-sea vents as revealed by randomly amplified polymorphic DNA fingerprinting and 16S rRNA gene sequence analysis. Appl Environ Microbiol. 2004;70:1277-86 pubmed
    ..gene sequences of representatives of each RAPD group and unique profiles showed that one group corresponds to Pyrococcus strains, whereas all the other isolates are Thermococcus strains...
  24. Zivanovic Y, Lopez P, Philippe H, Forterre P. Pyrococcus genome comparison evidences chromosome shuffling-driven evolution. Nucleic Acids Res. 2002;30:1902-10 pubmed
    The genomes of three Pyrococcus species, P.abyssi, P.furiosus and P.horikoshii, were compared at the DNA level, taking advantage of our identification of their replication origins...
  25. Ezaki S, Maeda N, Kishimoto T, Atomi H, Imanaka T. Presence of a structurally novel type ribulose-bisphosphate carboxylase/oxygenase in the hyperthermophilic archaeon, Pyrococcus kodakaraensis KOD1. J Biol Chem. 1999;274:5078-82 pubmed
    ..the gene encoding ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) of the hyperthermophilic archaeon, Pyrococcus kodakaraensis KOD1...
  26. Ishikawa K, Ishida H, Koyama Y, Kawarabayasi Y, Kawahara J, Matsui E, et al. Acylamino acid-releasing enzyme from the thermophilic archaeon Pyrococcus horikoshii. J Biol Chem. 1998;273:17726-31 pubmed
    When the genome of the thermophilic archaeon Pyrococcus horikoshii was sequenced, a gene homologous to the mammalian gene for an acylamino acid-releasing enzyme (EC 3.4.19...
  27. Ando S, Ishida H, Kosugi Y, Ishikawa K. Hyperthermostable endoglucanase from Pyrococcus horikoshii. Appl Environ Microbiol. 2002;68:430-3 pubmed
    An endoglucanase homolog from the hyperthermophilic archaeon Pyrococcus horikoshii was expressed in Escherichia coli, and its enzymatic characteristics were examined...
  28. Matsui E, Kawasaki S, Ishida H, Ishikawa K, Kosugi Y, Kikuchi H, et al. Thermostable flap endonuclease from the archaeon, Pyrococcus horikoshii, cleaves the replication fork-like structure endo/exonucleolytically. J Biol Chem. 1999;274:18297-309 pubmed
    The flap endonuclease gene homologue from the hyperthermophilic archaeon, Pyrococcus horikoshii, was overexpressed in Escherichia coli and purified. The results of gel filtration indicated that this protein was a 41-kDa monomer. P...
  29. Nolivos S, Carpousis A, Clouet d Orval B. The K-loop, a general feature of the Pyrococcus C/D guide RNAs, is an RNA structural motif related to the K-turn. Nucleic Acids Res. 2005;33:6507-14 pubmed
    The C/D guide RNAs predicted from the genomic sequences of three species of Pyrococcus delineate a family of small non-coding archaeal RNAs involved in the methylation of rRNA and tRNA...
  30. Tanaka T, Fujiwara S, Nishikori S, Fukui T, Takagi M, Imanaka T. A unique chitinase with dual active sites and triple substrate binding sites from the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1. Appl Environ Microbiol. 1999;65:5338-44 pubmed
    We have found that the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 produces an extracellular chitinase. The gene encoding the chitinase (chiA) was cloned and sequenced...
  31. Tahirov T, Oki H, Tsukihara T, Ogasahara K, Yutani K, Libeu C, et al. High-resolution crystals of methionine aminopeptidase from Pyrococcus furiosus obtained by water-mediated transformation. J Struct Biol. 1998;121:68-72 pubmed publisher
    The monoclinic crystal form of methionine amino-peptidase from Pyrococcus furiosus (MAP-Pfu) has been crystallized from four different conditions. Native crystals belong to space group P2(1) with typical unit-cell dimensions a = 53...
  32. Ito N, Nureki O, Shirouzu M, Yokoyama S, Hanaoka F. Crystallization and preliminary X-ray analysis of a DNA primase from hyperthermophilic archaeon Pyrococcus horikoshii. J Biochem. 2001;130:727-30 pubmed
    ..synthesis by archaeal/eukaryotic-type primases, the gene of the DNA primase from hyperthermophilic archaeon Pyrococcus horikoshii was cloned and overexpressed in Escherichia coli as a fusion protein with a hexa-histidine tag at its ..
  33. Postec A, Urios L, Lesongeur F, Ollivier B, Querellou J, Godfroy A. Continuous enrichment culture and molecular monitoring to investigate the microbial diversity of thermophiles inhabiting deep-sea hydrothermal ecosystems. Curr Microbiol. 2005;50:138-44 pubmed
    ..Archaeal and bacterial diversity was studied. Microorganisms affiliated with the genera Pyrococcus, Marinitoga, and Bacillus were detected through DGGE analysis of 16S rDNA...
  34. Empadinhas N, Marugg J, Borges N, Santos H, da Costa M. Pathway for the synthesis of mannosylglycerate in the hyperthermophilic archaeon Pyrococcus horikoshii. Biochemical and genetic characterization of key enzymes. J Biol Chem. 2001;276:43580-8 pubmed publisher
    ..pathway for the synthesis of the compatible solute alpha-mannosylglycerate in the hyperthermophilic archaeon Pyrococcus horikoshii is proposed based on the activities of purified recombinant mannosyl-3-phosphoglycerate (MPG) ..
  35. Ramos C, Oliveira C, Torriani I, Oliveira C. The Pyrococcus exosome complex: structural and functional characterization. J Biol Chem. 2006;281:6751-9 pubmed
    ..The results shown here also indicate the participation of the exosome in RNA metabolism in Archaea, as was established in Eukarya...
  36. Koning S, Elferink M, Konings W, Driessen A. Cellobiose uptake in the hyperthermophilic archaeon Pyrococcus furiosus is mediated by an inducible, high-affinity ABC transporter. J Bacteriol. 2001;183:4979-84 pubmed
    The hyperthermophilic archaeon Pyrococcus furiosus can utilize different beta-glucosides, like cellobiose and laminarin...
  37. Shen Y, Tang X, Matsui I. Subunit interaction and regulation of activity through terminal domains of the family D DNA polymerase from Pyrococcus horikoshii. J Biol Chem. 2003;278:21247-57 pubmed
    Functions of the terminal domains of the family D DNA polymerase from Pyrococcus horikoshii (PolDPho) were analyzed by making and characterizing various truncated proteins. Based on a co-expression vector developed previously (Shen, Y...
  38. Tang X, Ezaki S, Fujiwara S, Takagi M, Atomi H, Imanaka T. The tryptophan biosynthesis gene cluster trpCDEGFBA from Pyrococcus kodakaraensis KOD1 is regulated at the transcriptional level and expressed as a single mRNA. Mol Gen Genet. 1999;262:815-21 pubmed
    ..entire gene cluster encoding enzymes involved in biosynthesis of L-tryptophan in the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 has been cloned and sequenced...
  39. Takagi M, Nishioka M, Kakihara H, Kitabayashi M, Inoue H, Kawakami B, et al. Characterization of DNA polymerase from Pyrococcus sp. strain KOD1 and its application to PCR. Appl Environ Microbiol. 1997;63:4504-10 pubmed
    The DNA polymerase gene from the archaeon Pyrococcus sp. strain KOD1 (KOD DNA polymerase) contains a long open reading frame of 5,013 bases that encodes 1,671 amino acid residues (GenBank accession no. D29671)...
  40. Okochi M, Matsuzaki H, Nomura T, Ishii N, Yohda M. Molecular characterization of the group II chaperonin from the hyperthermophilic archaeum Pyrococcus horikoshii OT3. Extremophiles. 2005;9:127-34 pubmed
    The group II chaperonin from the hyperthermophilic archaeum Pyrococcus horikoshii OT3 (PhCPN) and its functional cooperation with the cognate prefoldin were investigated...
  41. Blamey J, Chiong M, Lopez C, Smith E. Optimization of the growth conditions of the extremely thermophilic microorganisms Thermococcus celer and Pyrococcus woesei. J Microbiol Methods. 1999;38:169-75 pubmed
    Growth medium components and cultivation conditions for the extremely thermophilic Archaea Thermococcus celer and Pyrococcus woesei were optimized. A culture media based in marine water was formulated...
  42. Cambillau C, Claverie J. Structural and genomic correlates of hyperthermostability. J Biol Chem. 2000;275:32383-6 pubmed
    ..a few bacteria have been found capable of withstanding temperatures close to 100 degrees C, or beyond, such as Pyrococcus or Aquifex...
  43. Matsui I, Matsui E, Sakai Y, Kikuchi H, Kawarabayasi Y, Ura H, et al. The molecular structure of hyperthermostable aromatic aminotransferase with novel substrate specificity from Pyrococcus horikoshii. J Biol Chem. 2000;275:4871-9 pubmed
    ..The crystal structure of this aminotransferase from the hyperthermophilic archaeon Pyrococcus horikoshii was determined to a resolution of 2.1 A...
  44. Schmitt E, Moulinier L, Fujiwara S, Imanaka T, Thierry J, Moras D. Crystal structure of aspartyl-tRNA synthetase from Pyrococcus kodakaraensis KOD: archaeon specificity and catalytic mechanism of adenylate formation. EMBO J. 1998;17:5227-37 pubmed publisher
    The crystal structure of aspartyl-tRNA synthetase (AspRS) from Pyrococcus kodakaraensis was solved at 1.9 A resolution...
  45. Gonz lez J, Masuchi Y, Robb F, Ammerman J, Maeder D, Yanagibayashi M, et al. Pyrococcus horikoshii sp. nov., a hyperthermophilic archaeon isolated from a hydrothermal vent at the Okinawa Trough. Extremophiles. 1998;2:123-30 pubmed
    ..4% (range 1%-5%). Tryptophan was required for growth, in contrast to the closely related strains Pyrococcus furiosus and P. abyssi...
  46. Li T, Sun F, Ji X, Feng Y, Rao Z. Structure based hyperthermostability of archaeal histone HPhA from Pyrococcus horikoshii. J Mol Biol. 2003;325:1031-7 pubmed
    The histone protein HPhA from the hyperthermophilic archaeon Pyrococcus horikoshii, shows hyperthermostability, as required for optimal growth of the organism at 95 degrees C. The structure of recombinant P...
  47. Pingoud V, Conzelmann C, Kinzebach S, Sudina A, Metelev V, Kubareva E, et al. PspGI, a type II restriction endonuclease from the extreme thermophile Pyrococcus sp.: structural and functional studies to investigate an evolutionary relationship with several mesophilic restriction enzymes. J Mol Biol. 2003;329:913-29 pubmed
    ..These results are discussed in an evolutionary context...
  48. van de Werken H, Verhees C, Akerboom J, de Vos W, van der Oost J. Identification of a glycolytic regulon in the archaea Pyrococcus and Thermococcus. FEMS Microbiol Lett. 2006;260:69-76 pubmed
    The glycolytic pathway of the hyperthermophilic archaea that belong to the order Thermococcales (Pyrococcus, Thermococcus and Palaeococcus) differs significantly from the canonical Embden-Meyerhof pathway in bacteria and eukarya...
  49. Hamilton Brehm S, Schut G, Adams M. Metabolic and evolutionary relationships among Pyrococcus Species: genetic exchange within a hydrothermal vent environment. J Bacteriol. 2005;187:7492-9 pubmed
    b>Pyrococcus furiosus and Pyrococcus woesei grow optimally at temperatures near 100 degrees C and were isolated from the same shallow marine volcanic vent system. Hybridization of genomic DNA from P...
  50. Jung J, Lee J, Holden J, Seo D, Shin H, Kim H, et al. Complete genome sequence of the hyperthermophilic archaeon Pyrococcus sp. strain ST04, isolated from a deep-sea hydrothermal sulfide chimney on the Juan de Fuca Ridge. J Bacteriol. 2012;194:4434-5 pubmed publisher
    b>Pyrococcus sp. strain ST04 is a hyperthermophilic, anaerobic, and heterotrophic archaeon isolated from a deep-sea hydrothermal sulfide chimney on the Endeavour Segment of the Juan de Fuca Ridge in the northeastern Pacific Ocean...
  51. Kecha M, Benallaoua S, Touzel J, Bonaly R, Duchiron F. Biochemical and phylogenetic characterization of a novel terrestrial hyperthermophilic archaeon pertaining to the genus Pyrococcus from an Algerian hydrothermal hot spring. Extremophiles. 2007;11:65-73 pubmed
    ..Sulfur is reduced to Hydrogen sulfide and enhances growth. It shares with other Pyrococcus species the heterotrophic mode of nutrition, the hyperthermophily, the ability to utilize amino acids as sole ..
  52. Akiba T, Nishio M, Matsui I, Harata K. X-ray structure of a membrane-bound beta-glycosidase from the hyperthermophilic archaeon Pyrococcus horikoshii. Proteins. 2004;57:422-31 pubmed publisher
    The beta-glycosidase of the hyperthermophilic Archaeon Pyrococcus horikoshii is a membrane-bound enzyme with the preferred substrate of alkyl-beta-glycosides...
  53. Wang S, Feng Y, Zhang Z, Zheng B, Li N, Cao S, et al. Heat effect on the structure and activity of the recombinant glutamate dehydrogenase from a hyperthermophilic archaeon Pyrococcus horikoshii. Arch Biochem Biophys. 2003;411:56-62 pubmed
    Glutamate dehydrogenase from Pyrococcus horikoshii (Pho-GDH) was cloned and overexpressed in Escherichia coli...
  54. Tsuge H, Sakuraba H, Kobe T, Kujime A, Katunuma N, Ohshima T. Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-A resolution: a large conformational change in ADP-dependent glucokinase. Protein Sci. 2002;11:2456-63 pubmed publisher
    ..the most common phosphoryl group donor for kinases, some kinases from certain hyperthermophilic archaea such as Pyrococcus horikoshii and Thermococcus litoralis use ADP as the phosphoryl donor...
  55. Liu L, Iwata K, Kita A, Kawarabayasi Y, Yohda M, Miki K. Crystal structure of aspartate racemase from Pyrococcus horikoshii OT3 and its implications for molecular mechanism of PLP-independent racemization. J Mol Biol. 2002;319:479-89 pubmed publisher
    ..We have determined the three-dimensional structure of aspartate racemase from the hyperthermophilic archaeum Pyrococcus horikoshii OT3 at 1.9 A resolution by X-ray crystallography and refined it to a crystallographic R factor of 19...
  56. Du X, Wang W, Kim R, Yakota H, Nguyen H, Kim S. Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii. Biochemistry. 2001;40:14166-72 pubmed
    ..tuberculosis. We have determined the crystal structure of the gene product of Pyrococcus horikoshii 999 (PH999), a PZAase, and its complex with zinc ion by X-ray crystallography...
  57. Horiike T, Hamada K, Miyata D, Shinozawa T. The origin of eukaryotes is suggested as the symbiosis of pyrococcus into gamma-proteobacteria by phylogenetic tree based on gene content. J Mol Evol. 2004;59:606-19 pubmed
    ..of this new method into the phylogenetic tree analysis of 66 organisms (4 eukaryotes, 13 archaea, and 49 eubacteria) based on gene content suggests the symbiosis of pyrococcus into gamma-proteobacteria as the origin of eukaryotes.
  58. Dabrowski S, Kiaer Ahring B. Cloning, expression, and purification of the His6-tagged hyper-thermostable dUTPase from Pyrococcus woesei in Escherichia coli: application in PCR. Protein Expr Purif. 2003;31:72-8 pubmed
    The gene encoding dUTPase from Pyrococcus woesei was cloned into Escherichia coli expression system. It shows 100% gene identity to homologous gene in Pyrococcus furiosus...
  59. Escobar Paramo P, Ghosh S, DiRuggiero J. Evidence for genetic drift in the diversification of a geographically isolated population of the hyperthermophilic archaeon Pyrococcus. Mol Biol Evol. 2005;22:2297-303 pubmed
    ..We used both phylogenetic and insertion sequence (IS) element analyses in hyperthermophilic archaea of the genus Pyrococcus to test the hypothesis that genetic drift played an important role in the diversification of these ..
  60. Lucas S, Toffin L, Zivanovic Y, Charlier D, Moussard H, Forterre P, et al. Construction of a shuttle vector for, and spheroplast transformation of, the hyperthermophilic archaeon Pyrococcus abyssi. Appl Environ Microbiol. 2002;68:5528-36 pubmed
    Our understanding of the genetics of species of the best-studied hyperthermophilic archaea, Pyrococcus spp...
  61. Watanabe M, Nameki N, Matsuo Takasaki M, Nishimura S, Okada N. tRNA recognition of tRNA-guanine transglycosylase from a hyperthermophilic archaeon, Pyrococcus horikoshii. J Biol Chem. 2001;276:2387-94 pubmed
    ..We examined the tRNA recognition of TGT from a hyperthermophilic archaeon, Pyrococcus horikoshii...
  62. Barbier G, Godfroy A, Meunier J, Querellou J, Cambon M, Lesongeur F, et al. Pyrococcus glycovorans sp. nov., a hyperthermophilic archaeon isolated from the East Pacific Rise. Int J Syst Bacteriol. 1999;49 Pt 4:1829-37 pubmed
    ..Sequencing of the 16S rDNA gene showed that strain AL585T belonged to the genus Pyrococcus and was probably a new species. This was confirmed by total DNA hybridization...
  63. Rahman R, Fujiwara S, Takagi M, Imanaka T. Sequence analysis of glutamate dehydrogenase (GDH) from the hyperthermophilic archaeon Pyrococcus sp. KOD1 and comparison of the enzymatic characteristics of native and recombinant GDHs. Mol Gen Genet. 1998;257:338-47 pubmed
    The gdhA gene encoding glutamate dehydrogenase (GDH) from the hyperthermophilic archaeon Pyrococcus sp. KOD1 was cloned and sequenced...
  64. Zeng X, Birrien J, Fouquet Y, Cherkashov G, Jebbar M, Querellou J, et al. Pyrococcus CH1, an obligate piezophilic hyperthermophile: extending the upper pressure-temperature limits for life. ISME J. 2009;3:873-6 pubmed publisher
    ..Strain CH1 was successfully isolated and assigned to the genus Pyrococcus, within the Euryarchaeota lineage within the Archaea domain...
  65. Leclere M, Nishioka M, Yuasa T, Fujiwara S, Takagi M, Imanaka T. The O6-methylguanine-DNA methyltransferase from the hyperthermophilic archaeon Pyrococcus sp. KOD1: a thermostable repair enzyme. Mol Gen Genet. 1998;258:69-77 pubmed
    ..of MGMTs from 11 species, we isolated the DNA region coding for MGMT from the hyperthermophilic archaeon Pyrococcus sp. KOD1...
  66. Gueguen Y, Voorhorst W, van der Oost J, de Vos W. Molecular and biochemical characterization of an endo-beta-1,3- glucanase of the hyperthermophilic archaeon Pyrococcus furiosus. J Biol Chem. 1997;272:31258-64 pubmed
    We report here the first molecular characterization of an endo-beta-1,3-glucanase from an archaeon. Pyrococcus furiosus is a hyperthermophilic archaeon that is capable of saccharolytic growth...