methanosarcina barkeri

Summary

Summary: A species of halophilic archaea whose organisms are nonmotile. Habitats include freshwater and marine mud, animal-waste lagoons, and the rumens of ungulates.

Top Publications

  1. Liu J, Marison I, von Stockar U. Microbial growth by a net heat up-take: a calorimetric and thermodynamic study on acetotrophic methanogenesis by Methanosarcina barkeri. Biotechnol Bioeng. 2001;75:170-80 pubmed
    ..exists, and in particular, to verify Heijnen and van Dijken's prediction (1992), acetotrophic methanogen, Methanosarcina barkeri, has been cultivated in a highly sensitive bench-scale calorimeter (an improved Bio-RC1 reaction ..
  2. Tallant T, Paul L, Krzycki J. The MtsA subunit of the methylthiol:coenzyme M methyltransferase of Methanosarcina barkeri catalyses both half-reactions of corrinoid-dependent dimethylsulfide: coenzyme M methyl transfer. J Biol Chem. 2001;276:4485-93 pubmed
  3. Heiden S, Hedderich R, Setzke E, Thauer R. Purification of a two-subunit cytochrome-b-containing heterodisulfide reductase from methanol-grown Methanosarcina barkeri. Eur J Biochem. 1994;221:855-61 pubmed
    ..The heterodisulfide reductase activity of the membrane fraction of methanol-grown Methanosarcina barkeri was solubilized by Chaps...
  4. Théobald Dietrich A, Frugier M, Giege R, Rudinger Thirion J. Atypical archaeal tRNA pyrrolysine transcript behaves towards EF-Tu as a typical elongator tRNA. Nucleic Acids Res. 2004;32:1091-6 pubmed
    ..specific incorporation of pyrrolysine in the active site of methylamine methyltransferases in the archaeon Methanosarcina barkeri. In solution probing experiments, a transcript derived from tRNA(Pyl) displays a secondary fold slightly ..
  5. Chien Y, Auerbuch V, Brabban A, Zinder S. Analysis of genes encoding an alternative nitrogenase in the archaeon Methanosarcina barkeri 227. J Bacteriol. 2000;182:3247-53 pubmed
    b>Methanosarcina barkeri 227 possesses two clusters of genes potentially encoding nitrogenases...
  6. Wassenaar R, Keltjens J, van der Drift C, Vogels G. Purification and characterization of dimethylamine:5-hydroxybenzimidazolyl-cobamide methyltransferase from Methanosarcina barkeri Fusaro. Eur J Biochem. 1998;253:692-7 pubmed
    Dimethylamine:5-hydroxybenzimidazolylcobamide methyltransferase (DMA-MT) was purified from cells of Methanosarcina barkeri Fusaro grown on trimethylamine...
  7. Feist A, Scholten J, Palsson B, Brockman F, Ideker T. Modeling methanogenesis with a genome-scale metabolic reconstruction of Methanosarcina barkeri. Mol Syst Biol. 2006;2:2006.0004 pubmed
    We present a genome-scale metabolic model for the archaeal methanogen Methanosarcina barkeri. We characterize the metabolic network and compare it to reconstructions from the prokaryotic, eukaryotic and archaeal domains...
  8. Ferguson D, Krzycki J. Reconstitution of trimethylamine-dependent coenzyme M methylation with the trimethylamine corrinoid protein and the isozymes of methyltransferase II from Methanosarcina barkeri. J Bacteriol. 1997;179:846-52 pubmed
  9. Polycarpo C, Ambrogelly A, Bérubé A, Winbush S, McCloskey J, Crain P, et al. An aminoacyl-tRNA synthetase that specifically activates pyrrolysine. Proc Natl Acad Sci U S A. 2004;101:12450-4 pubmed
    Pyrrolysine, the 22nd cotranslationally inserted amino acid, was found in the Methanosarcina barkeri monomethylamine methyltransferase protein in a position that is encoded by an in-frame UAG stop codon in the mRNA. M...

More Information

Publications70

  1. Korencic D, Polycarpo C, Weygand Durasevic I, S ll D. Differential modes of transfer RNASer recognition in Methanosarcina barkeri. J Biol Chem. 2004;279:48780-6 pubmed publisher
    Two dissimilar seryl-transfer RNA (tRNA) synthetases (SerRSs) exist in Methanosarcina barkeri, one of bacterial type and the other resembling SerRSs present only in some methanogenic archaea...
  2. Shima S, Netrusov A, Sordel M, Wicke M, Hartmann G, Thauer R. Purification, characterization, and primary structure of a monofunctional catalase from Methanosarcina barkeri. Arch Microbiol. 1999;171:317-23 pubmed
    b>Methanosarcina barkeri is a strictly anaerobic, cytochrome-containing, methane-forming archaeon. We report here that the microorganism contains a catalase, which was purified and characterized...
  3. Bilokapic S, Ivic N, Godinic Mikulcic V, Piantanida I, Ban N, Weygand Durasevic I. Idiosyncratic helix-turn-helix motif in Methanosarcina barkeri seryl-tRNA synthetase has a critical architectural role. J Biol Chem. 2009;284:10706-13 pubmed publisher
    ..The recently solved three-dimensional structure of Methanosarcina barkeri SerRS revealed the idiosyncratic features of methanogenic-type SerRSs; that is, an active site zinc ion, a ..
  4. Scholten J, Culley D, Brockman F, Wu G, Zhang W. Evolution of the syntrophic interaction between Desulfovibrio vulgaris and Methanosarcina barkeri: Involvement of an ancient horizontal gene transfer. Biochem Biophys Res Commun. 2007;352:48-54 pubmed
    The sulfate reducing bacteria Desulfovibrio vulgaris and the methanogenic archaea Methanosarcina barkeri can grow syntrophically on lactate. In this study, a set of three closely located genes, DVU2103, DVU2104, and DVU2108 of D...
  5. Tallant T, Krzycki J. Coenzyme M methylase activity of the 480-kilodalton corrinoid protein from Methanosarcina barkeri. J Bacteriol. 1996;178:1295-301 pubmed
    Activity staining of extracts of Methanosarcina barkeri electrophoresed in polyacrylamide gels revealed an additional methylcobalamin:coenzyme M (methylcobalamin:CoM) methyltransferase present in cells grown on acetate but not in those ..
  6. Brioukhanov A, Netrusov A, Eggen R. The catalase and superoxide dismutase genes are transcriptionally up-regulated upon oxidative stress in the strictly anaerobic archaeon Methanosarcina barkeri. Microbiology. 2006;152:1671-7 pubmed publisher
    b>Methanosarcina barkeri is a strictly anaerobic methanogenic archaeon, which can survive oxidative stress. The oxidative stress agent paraquat (PQ) suppressed growth of M. barkeri at concentrations of 50-200 microM...
  7. Culley D, Kovacik W, Brockman F, Zhang W. Optimization of RNA isolation from the archaebacterium Methanosarcina barkeri and validation for oligonucleotide microarray analysis. J Microbiol Methods. 2006;67:36-43 pubmed
    The recent completion of a draft genome sequence for Methanosarcina barkeri has allowed the application of various high throughput post-genomics technologies, such as nucleic acid microarrays and mass spectrometry of proteins to detect ..
  8. Chien Y, Zinder S. Cloning, DNA sequencing, and characterization of a nifD-homologous gene from the archaeon Methanosarcina barkeri 227 which resembles nifD1 from the eubacterium Clostridium pasteurianum. J Bacteriol. 1994;176:6590-8 pubmed
    ..Microbiol. 142:5-12, 1991) cloned and sequenced two nifH-homologous open reading frames (ORFs) from Methanosarcina barkeri 227. Phylogenetic analysis of the deduced amino acid sequences of the nifH ORFs from M...
  9. Paul L, Krzycki J. Sequence and transcript analysis of a novel Methanosarcina barkeri methyltransferase II homolog and its associated corrinoid protein homologous to methionine synthase. J Bacteriol. 1996;178:6599-607 pubmed
    The sequence and transcript of the genes encoding a recently discovered coenzyme M methylase in Methanosarcina barkeri were analyzed...
  10. Welander P, Metcalf W. Loss of the mtr operon in Methanosarcina blocks growth on methanol, but not methanogenesis, and reveals an unknown methanogenic pathway. Proc Natl Acad Sci U S A. 2005;102:10664-9 pubmed
    In the methanogenic archaeon Methanosarcina barkeri Fusaro, the N5-methyl-tetrahydrosarcinapterin (CH3-H4SPT):coenzyme M (CoM) methyltransferase, encoded by the mtr operon, catalyzes the energy-conserving (sodium-pumping) methyl transfer ..
  11. Banik S, Bandyopadhyay S, Ganguly S, Dan D. Effect of microwave irradiated Methanosarcina barkeri DSM-804 on biomethanation. Bioresour Technol. 2006;97:819-23 pubmed
    b>Methanosarcina barkeri DSM-804, a methanogenic bacterium, when exposed to microwave radiation of frequencies ranging from 13.5 to 36.5 GHz, showed faster growth in comparison to the unirradiated bacterial culture...
  12. Plugge C, Scholten J, Culley D, Nie L, Brockman F, Zhang W. Global transcriptomics analysis of the Desulfovibrio vulgaris change from syntrophic growth with Methanosarcina barkeri to sulfidogenic metabolism. Microbiology. 2010;156:2746-56 pubmed publisher
    ..vulgaris shifted its metabolism from syntrophic in a lactate-oxidizing dual-culture with Methanosarcina barkeri to a sulfidogenic metabolism...
  13. Ambrogelly A, Gundllapalli S, Herring S, Polycarpo C, Frauer C, Soll D. Pyrrolysine is not hardwired for cotranslational insertion at UAG codons. Proc Natl Acad Sci U S A. 2007;104:3141-6 pubmed
    ..Forty-two Methanosarcina barkeri tRNA(Pyl) variants were tested in Escherichia coli for suppression of the lac amber A24 mutation; then ..
  14. Neumann H, Peak Chew S, Chin J. Genetically encoding N(epsilon)-acetyllysine in recombinant proteins. Nat Chem Biol. 2008;4:232-4 pubmed publisher
    ..This strategy should find wide applications in defining the cellular role of this modification...
  15. Maeder D, Anderson I, Brettin T, Bruce D, Gilna P, Han C, et al. The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes. J Bacteriol. 2006;188:7922-31 pubmed
    We report here a comparative analysis of the genome sequence of Methanosarcina barkeri with those of Methanosarcina acetivorans and Methanosarcina mazei. The genome of M...
  16. Zhang W, Culley D, Nie L, Brockman F. DNA microarray analysis of anaerobic Methanosarcina barkeri reveals responses to heat shock and air exposure. J Ind Microbiol Biotechnol. 2006;33:784-90 pubmed
    b>Methanosarcina barkeri is a methanogenic archaeon that can digest cellulose and other polysaccharides to produce methane. It can only grow under strictly anoxic conditions, but which can survive air exposure...
  17. Vorholt J, Vaupel M, Thauer R. A polyferredoxin with eight [4Fe-4S] clusters as a subunit of molybdenum formylmethanofuran dehydrogenase from Methanosarcina barkeri. Eur J Biochem. 1996;236:309-17 pubmed
    Formylmethanofuran dehydrogenase (Fmd) from Methanosarcina barkeri is a molybdenum iron-sulfur protein involved in methanogenesis. The enzyme contains approximately 30 mol non-heme iron/mol and 30 mol acid-labile sulfur/mol...
  18. Buchenau B, Kahnt J, Heinemann I, Jahn D, Thauer R. Heme biosynthesis in Methanosarcina barkeri via a pathway involving two methylation reactions. J Bacteriol. 2006;188:8666-8 pubmed
    The methanogenic archaeon Methanosarcina barkeri synthesizes protoheme via precorrin-2, which is formed from uroporphyrinogen III in two consecutive methylation reactions utilizing S-adenosyl-L-methionine...
  19. Mukhopadhyay B, Purwantini E, Kreder C, Wolfe R. Oxaloacetate synthesis in the methanarchaeon Methanosarcina barkeri: pyruvate carboxylase genes and a putative Escherichia coli-type bifunctional biotin protein ligase gene (bpl/birA) exhibit a unique organization. J Bacteriol. 2001;183:3804-10 pubmed publisher
    Evidence is presented that, in Methanosarcina barkeri oxaloacetate synthesis, an essential and major CO(2) fixation reaction is catalyzed by an apparent alpha(4)beta(4)-type acetyl coenzyme A-independent pyruvate carboxylase (PYC), ..
  20. Gencic S, LeClerc G, Gorlatova N, Peariso K, Penner Hahn J, Grahame D. Zinc-thiolate intermediate in catalysis of methyl group transfer in Methanosarcina barkeri. Biochemistry. 2001;40:13068-78 pubmed
    ..methyl group transfer was studied for the methyltransferase enzyme MT2-A important for methanogenesis in Methanosarcina barkeri growing on methylamines...
  21. Ferguson D, Krzycki J, Grahame D. Specific roles of methylcobamide:coenzyme M methyltransferase isozymes in metabolism of methanol and methylamines in Methanosarcina barkeri. J Biol Chem. 1996;271:5189-94 pubmed
    ..Specific removal of the MT2 isozymes from buffer soluble cell extracts of Methanosarcina barkeri was accomplished by use of immobilized, affinity-purified, ovine polyclonal antibodies...
  22. Bock A, Kunow J, Glasemacher J, Sch nheit P. Catalytic properties, molecular composition and sequence alignments of pyruvate: ferredoxin oxidoreductase from the methanogenic archaeon Methanosarcina barkeri (strain Fusaro). Eur J Biochem. 1996;237:35-44 pubmed
    b>Methanosarcina barkeri (strain Fusaro) was grown on pyruvate as methanogenic substrate [Bock, A. K., Prieger-Kraft, A. & Schönheit, P. (1994) Arch. Microbiol. 161, 33-46]...
  23. Wassenaar R, Daas P, Geerts W, Keltjens J, van der Drift C. Involvement of methyltransferase-activating protein and methyltransferase 2 isoenzyme II in methylamine:coenzyme M methyltransferase reactions in Methanosarcina barkeri Fusaro. J Bacteriol. 1996;178:6937-44 pubmed
    ..tri- and dimethylamine to 2-mercaptoethanesulfonic acid (coenzyme M) were resolved from cell extracts of Methanosarcina barkeri Fusaro grown on methanol and trimethylamine, respectively...
  24. Boccazzi P, Zhang J, Metcalf W. Generation of dominant selectable markers for resistance to pseudomonic acid by cloning and mutagenesis of the ileS gene from the archaeon Methanosarcina barkeri fusaro. J Bacteriol. 2000;182:2611-8 pubmed
    ..acid (PA(r)) in Methanosarcina species by mutagenesis of the isoleucyl-tRNA synthetase gene (ileS) from Methanosarcina barkeri Fusaro. The M...
  25. K nkel A, Vaupel M, Heim S, Thauer R, Hedderich R. Heterodisulfide reductase from methanol-grown cells of Methanosarcina barkeri is not a flavoenzyme. Eur J Biochem. 1997;244:226-34 pubmed
    Heterodisulfide reductase from methanol-grown cells of Methanosarcina barkeri (MbHdrDE) is a membrane-bound enzyme composed of a 46-kDa subunit MbHdrD and a 23-kDa subunit MbHdrE. The enzyme has been shown to contain 0...
  26. Meuer J, Kuettner H, Zhang J, Hedderich R, Metcalf W. Genetic analysis of the archaeon Methanosarcina barkeri Fusaro reveals a central role for Ech hydrogenase and ferredoxin in methanogenesis and carbon fixation. Proc Natl Acad Sci U S A. 2002;99:5632-7 pubmed
    Ech hydrogenase (Ech) from the methanogenic archaeon Methanosarcina barkeri catalyzes the reversible reduction of ferredoxin by H(2) and is a member of a distinct group of membrane-bound [NiFe] hydrogenases with sequence similarity to ..
  27. Metcalf W, Zhang J, Shi X, Wolfe R. Molecular, genetic, and biochemical characterization of the serC gene of Methanosarcina barkeri Fusaro. J Bacteriol. 1996;178:5797-802 pubmed
    The Methanosarcina barkeri serC gene, encoding phosphoserine aminotransferase, was cloned by complementation of an Escherichia coli serC mutant, and its nucleotide sequence was determined. The M...
  28. Sauer K, Harms U, Thauer R. Methanol:coenzyme M methyltransferase from Methanosarcina barkeri. Purification, properties and encoding genes of the corrinoid protein MT1. Eur J Biochem. 1997;243:670-7 pubmed
    In Methanosarcina barkeri, methanogenesis from methanol is initiated by the formation of methylcoenzyme M from methanol and coenzyme M. This methyl transfer reaction is catalyzed by two enzymes, designated MT1 and MT2...
  29. Sumi M, Yohda M, Koga Y, Yoshida M. F0F1-ATPase genes from an archaebacterium, Methanosarcina barkeri. Biochem Biophys Res Commun. 1997;241:427-33 pubmed publisher
    It has been known that an archaebacterium Methanosarcina barkeri strain MS (DSM 800) has a V-type ATPase (Inatomi, K., et al. (1989) J. Biol. Chem. 264, 10954-10959)...
  30. Buchenau B, Thauer R. Tetrahydrofolate-specific enzymes in Methanosarcina barkeri and growth dependence of this methanogenic archaeon on folic acid or p-aminobenzoic acid. Arch Microbiol. 2004;182:313-25 pubmed publisher
    ..The presence of both H4SPT and H4F in M. barkeri is in agreement with earlier isotope labeling studies indicating that there are two separate C1 pools in these methanogens...
  31. Forzi L, Koch J, Guss A, Radosevich C, Metcalf W, Hedderich R. Assignment of the [4Fe-4S] clusters of Ech hydrogenase from Methanosarcina barkeri to individual subunits via the characterization of site-directed mutants. FEBS J. 2005;272:4741-53 pubmed
    Ech hydrogenase from Methanosarcina barkeri is a member of a distinct group of membrane-bound [NiFe] hydrogenases with sequence similarity to energy-conserving NADH:quinone oxidoreductase (complex I)...
  32. Welander P, Metcalf W. Mutagenesis of the C1 oxidation pathway in Methanosarcina barkeri: new insights into the Mtr/Mer bypass pathway. J Bacteriol. 2008;190:1928-36 pubmed publisher
    A series of Methanosarcina barkeri mutants lacking the genes encoding the enzymes involved in the C1 oxidation/reduction pathway were constructed...
  33. Guss A, Kulkarni G, Metcalf W. Differences in hydrogenase gene expression between Methanosarcina acetivorans and Methanosarcina barkeri. J Bacteriol. 2009;191:2826-33 pubmed publisher
    ..Comparison of the amino acid sequences of these putative hydrogenases to those of Methanosarcina barkeri and Methanosarcina mazei shows that each predicted subunit contains all the known residues essential for ..
  34. Ferguson D, Gorlatova N, Grahame D, Krzycki J. Reconstitution of dimethylamine:coenzyme M methyl transfer with a discrete corrinoid protein and two methyltransferases purified from Methanosarcina barkeri. J Biol Chem. 2000;275:29053-60 pubmed publisher
    ..These proteins isolated from Methanosarcina barkeri included the previously unidentified corrinoid protein MtbC, which copurified with MtbA, the ..
  35. Gong W, Hao B, Wei Z, Ferguson D, Tallant T, Krzycki J, et al. Structure of the alpha2epsilon2 Ni-dependent CO dehydrogenase component of the Methanosarcina barkeri acetyl-CoA decarbonylase/synthase complex. Proc Natl Acad Sci U S A. 2008;105:9558-63 pubmed publisher
  36. Anderson K, Apolinario E, Sowers K. Desiccation as a long-term survival mechanism for the archaeon Methanosarcina barkeri. Appl Environ Microbiol. 2012;78:1473-9 pubmed publisher
    ..In this study, we quantified the survival rates of the methanogenic archaeon Methanosarcina barkeri after desiccation under conditions equivalent to the driest environments on Earth and subsequent exposure ..
  37. Bock A, Schonheit P. Growth of Methanosarcina barkeri (Fusaro) under nonmethanogenic conditions by the fermentation of pyruvate to acetate: ATP synthesis via the mechanism of substrate level phosphorylation. J Bacteriol. 1995;177:2002-7 pubmed
    A mutant of Methanosarcina barkeri (Fusaro) is able to grow on pyruvate as the sole carbon and energy source. During growth, pyruvate is converted to CH4 and CO2, and about 1.5 mol of ATP per mol of CH4 is formed (A.-K. Bock, A...
  38. Gonnerman M, Benedict M, Feist A, Metcalf W, Price N. Genomically and biochemically accurate metabolic reconstruction of Methanosarcina barkeri Fusaro, iMG746. Biotechnol J. 2013;8:1070-9 pubmed publisher
    b>Methanosarcina barkeri is an Archaeon that produces methane anaerobically as the primary byproduct of its metabolism. M...
  39. Chien Y, Zinder S. Cloning, functional organization, transcript studies, and phylogenetic analysis of the complete nitrogenase structural genes (nifHDK2) and associated genes in the archaeon Methanosarcina barkeri 227. J Bacteriol. 1996;178:143-8 pubmed
    Determination of the nucleotide sequence of the nitrogenase structural genes (nifHDK2) from Methanosarcina barkeri 227 was completed in this study by cloning and sequencing a 2...
  40. Srinivasan G, James C, Krzycki J. Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA. Science. 2002;296:1459-62 pubmed
    Pyrrolysine is a lysine derivative encoded by the UAG codon in methylamine methyltransferase genes of Methanosarcina barkeri. Near a methyltransferase gene cluster is the pylT gene, which encodes an unusual transfer RNA (tRNA) with a CUA ..
  41. LeClerc G, Grahame D. Methylcobamide:coenzyme M methyltransferase isozymes from Methanosarcina barkeri. Physicochemical characterization, cloning, sequence analysis, and heterologous gene expression. J Biol Chem. 1996;271:18725-31 pubmed
    ..b>Methanosarcina barkeri genes cmtA and cmtM encoding both isozymes were cloned and sequenced...
  42. Harms U, Thauer R. Methylcobalamin: coenzyme M methyltransferase isoenzymes MtaA and MtbA from Methanosarcina barkeri. Cloning, sequencing and differential transcription of the encoding genes, and functional overexpression of the mtaA gene in Escherichia coli. Eur J Biochem. 1996;235:653-9 pubmed
    b>Methanosarcina barkeri is known to contain two methyltransferase isoenzymes, here designated MtaA and MtbA, which catalyze the formation of methyl-coenzyme M from methylcobalamin and coenzyme M...
  43. Kulkarni G, Kridelbaugh D, Guss A, Metcalf W. Hydrogen is a preferred intermediate in the energy-conserving electron transport chain of Methanosarcina barkeri. Proc Natl Acad Sci U S A. 2009;106:15915-20 pubmed publisher
    ..However, Methanosarcina barkeri Delta fpo mutants constructed in this study display no measurable phenotype on this substrate, indicating ..
  44. Paul L, Ferguson D, Krzycki J. The trimethylamine methyltransferase gene and multiple dimethylamine methyltransferase genes of Methanosarcina barkeri contain in-frame and read-through amber codons. J Bacteriol. 2000;182:2520-9 pubmed
    ..Each of the three DMA methyltransferase gene copies from Methanosarcina barkeri contained an amber codon at the same position, followed by a downstream UAA or UGA codon...
  45. Baumann T, Exner M, Budisa N. Orthogonal Protein Translation Using Pyrrolysyl-tRNA Synthetases for Single- and Multiple-Noncanonical Amino Acid Mutagenesis. Adv Biochem Eng Biotechnol. 2018;162:1-19 pubmed publisher
    ..translation are based on the Methanococcus jannaschii TyrRS/tRNA CUATyr and the Methanosarcina barkeri/Methanosarcina mazei PylRS/tRNA CUAPyl pairs...
  46. López Muñoz M, Schönheit P, Metcalf W. Genetic, Genomic, and Transcriptomic Studies of Pyruvate Metabolism in Methanosarcina barkeri Fusaro. J Bacteriol. 2015;197:3592-600 pubmed publisher
    ..The sole exception to this rule is a genetically uncharacterized Methanosarcina barkeri mutant capable of using pyruvate as a sole energy and carbon source (the Pyr(+) phenotype)...
  47. Sekiguchi Y, Uyeno Y, Sunaga A, Yoshida H, Kamagata Y. Sequence-specific cleavage of 16S rRNA for rapid and quantitative detection of particular groups of anaerobes in bioreactors. Water Sci Technol. 2005;52:107-13 pubmed
    ..The results demonstrated that the present method was fully applicable to anaerobic digestor ecosystems containing complex anaerobic microorganisms...
  48. Chattopadhyay B, Thakur A. Inhibition of methanogenesis by interaction of aluminium ion with co-factor, F-420, in Methanosarcina barkeri. Indian J Exp Biol. 2003;41:915-7 pubmed
    ..Addition of Al3+ (20 mM) to the culture medium containing cells of pure Methanosarcina barkeri, inhibited methanogenesis...
  49. Kung Y, Doukov T, Seravalli J, Ragsdale S, Drennan C. Crystallographic snapshots of cyanide- and water-bound C-clusters from bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase. Biochemistry. 2009;48:7432-40 pubmed publisher
    ..The crystal structures presented here, along with recent structures of CODHs from other organisms, have led us toward a unified mechanism for CO oxidation by the C-cluster, the catalytic center of an environmentally important enzyme. ..
  50. Stojanowic A, Hedderich R. CO2 reduction to the level of formylmethanofuran in Methanosarcina barkeri is non-energy driven when CO is the electron donor. FEMS Microbiol Lett. 2004;235:163-7 pubmed
    A Methanosarcina barkeri mutant lacking Ech hydrogenase does not catalyze CH(4) formation from H(2)/CO(2) since, as was shown previously, the energy-driven reduction of CO(2) to formylmethanofuran by H(2) is blocked...
  51. Lee H, Dominy B, Cao W. New family of deamination repair enzymes in uracil-DNA glycosylase superfamily. J Biol Chem. 2011;286:31282-7 pubmed publisher
    ..This study demonstrates the structural evolvability that underlies substrate specificity and catalytic flexibility in the evolution of enzymatic function...
  52. Pfl ger K, Baumann S, Gottschalk G, Lin W, Santos H, M ller V. Lysine-2,3-aminomutase and beta-lysine acetyltransferase genes of methanogenic archaea are salt induced and are essential for the biosynthesis of Nepsilon-acetyl-beta-lysine and growth at high salinity. Appl Environ Microbiol. 2003;69:6047-55 pubmed
    ..on the chromosomes of the methanogenic archaea Methanosarcina mazei Gö1, Methanosarcina acetivorans, Methanosarcina barkeri, Methanococcus jannaschii, and Methanococcus maripaludis...
  53. Bilokapic S, Maier T, Ahel D, Gruic Sovulj I, S ll D, Weygand Durasevic I, et al. Structure of the unusual seryl-tRNA synthetase reveals a distinct zinc-dependent mode of substrate recognition. EMBO J. 2006;25:2498-509 pubmed publisher
    ..Here, we report a 2.5 A resolution crystal structure of the atypical methanogenic Methanosarcina barkeri SerRS and its complexes with ATP, serine and the nonhydrolysable seryl-adenylate analogue 5'-O-(N-..
  54. WOLFE R, Metcalf W. A vacuum-vortex technique for preparation of anoxic solutions or liquid culture media in small volumes for cultivating methanogens or other strict anaerobes. Anaerobe. 2010;16:216-9 pubmed publisher
    ..Liquid media suitable for the cultivation of methanogens may be rendered anoxic after three short vacuum-vortex steps...
  55. Burke S, Lo S, Krzycki J. Clustered genes encoding the methyltransferases of methanogenesis from monomethylamine. J Bacteriol. 1998;180:3432-40 pubmed
    ..The genes for these proteins are clustered on 6.8 kb of DNA in Methanosarcina barkeri MS. The gene encoding MMCP (mtmC) is located directly upstream of the gene encoding MMAMT (mtmB)...
  56. Burke S, Krzycki J. Involvement of the "A" isozyme of methyltransferase II and the 29-kilodalton corrinoid protein in methanogenesis from monomethylamine. J Bacteriol. 1995;177:4410-6 pubmed
    ..A likely mechanism is that the 29-kDa corrinoid is methylated by MMA and the methyl group is then transferred by the "A" isozyme of MT2 to CoM...
  57. Gundllapalli S, Ambrogelly A, Umehara T, Li D, Polycarpo C, Soll D. Misacylation of pyrrolysine tRNA in vitro and in vivo. FEBS Lett. 2008;582:3353-8 pubmed publisher
    b>Methanosarcina barkeri inserts pyrrolysine (Pyl) at an in-frame UAG codon in its monomethylamine methyltransferase gene. Pyrrolysyl-tRNA synthetase acylates Pyl onto tRNAPyl, the amber suppressor pyrrolysine Pyl tRNA. Here we show that M...
  58. Friedrich M. Methyl-coenzyme M reductase genes: unique functional markers for methanogenic and anaerobic methane-oxidizing Archaea. Methods Enzymol. 2005;397:428-42 pubmed
    ..It has also been suggested that anaerobic methane-oxidizing Archaea possess MCR, which facilitates detection of this novel group of "reverse methanogens" as well using the mcrA gene as a functional marker...
  59. BUAN N, Kulkarni G, METCALF W. Genetic methods for methanosarcina species. Methods Enzymol. 2011;494:23-42 pubmed publisher
    ..discussed in this chapter form the basis for all genetic experiments in Methanosarcina acetivorans C2A and Methanosarcina barkeri Fusaro, two methanogens that are routinely used as model organisms for genetic experiments...
  60. Schlegel K, Muller V. Sodium ion translocation and ATP synthesis in methanogens. Methods Enzymol. 2011;494:233-55 pubmed publisher
    ..two model methanogens, the hydrogenotrophic Methanothermobacter thermautotrophicus and the methylotrophic Methanosarcina barkeri. Furthermore, the determination of important bioenergetic parameters like the ?pH, ??, or the ..
  61. Li T, Graham D, Stathopoulos C, Haney P, Kim H, Vothknecht U, et al. Cysteinyl-tRNA formation: the last puzzle of aminoacyl-tRNA synthesis. FEBS Lett. 1999;462:302-6 pubmed
    ..coli cysSts mutant reveal that Methanococcus maripaludis and Methanosarcina barkeri, two other methanogenic archaea, possess canonical CysRS proteins...