methanosarcina

Summary

Summary: A genus of anaerobic, irregular spheroid-shaped METHANOSARCINALES whose organisms are nonmotile. Endospores are not formed. These archaea derive energy via formation of methane from acetate, methanol, mono-, di-, and trimethylamine, and possibly, carbon monoxide. Organisms are isolated from freshwater and marine environments.

Top Publications

  1. Li Q, Li L, Rejtar T, Lessner D, Karger B, Ferry J. Electron transport in the pathway of acetate conversion to methane in the marine archaeon Methanosarcina acetivorans. J Bacteriol. 2006;188:702-10 pubmed
    ..of proteins in acetate-grown cells compared to that of proteins in methanol-grown cells of the marine isolate Methanosarcina acetivorans metabolically labeled with 14N versus 15N. The 246 differentially abundant proteins in M...
  2. Longstaff D, Larue R, Faust J, Mahapatra A, Zhang L, Green Church K, et al. A natural genetic code expansion cassette enables transmissible biosynthesis and genetic encoding of pyrrolysine. Proc Natl Acad Sci U S A. 2007;104:1021-6 pubmed
    ..PylS-based assays demonstrated that pyrrolysine was biosynthesized in Escherichia coli expressing pylBCD from Methanosarcina acetivorans. Production of pyrrolysine did not require tRNA(Pyl) or PylS...
  3. Heimann A, Batstone D, Jakobsen R. Methanosarcina spp. drive vinyl chloride dechlorination via interspecies hydrogen transfer. Appl Environ Microbiol. 2006;72:2942-9 pubmed
    ..aceticlastic methanogens, one culture was dominated by Methanosaeta, while the other culture was dominated by Methanosarcina, as determined by fluorescence in situ hybridization...
  4. Lira Silva E, Santiago Martínez M, Hernández Juárez V, García Contreras R, Moreno Sanchez R, Jasso Chávez R. Activation of methanogenesis by cadmium in the marine archaeon Methanosarcina acetivorans. PLoS ONE. 2012;7:e48779 pubmed publisher
    b>Methanosarcina acetivorans was cultured in the presence of CdCl(2) to determine the metal effect on cell growth and biogas production...
  5. Mukai T, Kobayashi T, Hino N, Yanagisawa T, Sakamoto K, Yokoyama S. Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases. Biochem Biophys Res Commun. 2008;371:818-22 pubmed publisher
    ..in mammalian cells, based on the expression of the pyrrolysyl-tRNA synthetase (PylRS)-tRNA(Pyl) pair from Methanosarcina mazei...
  6. Clements A, Ferry J. Cloning, nucleotide sequence, and transcriptional analyses of the gene encoding a ferredoxin from Methanosarcina thermophila. J Bacteriol. 1992;174:5244-50 pubmed
    A mixed 17-mer oligonucleotide deduced from the N terminus of a ferredoxin isolated from Methanosarcina thermophila was used to probe a lambda gt11 library prepared from M. thermophila genomic DNA; positive clones contained either a 5...
  7. Weidenbach K, Glöer J, Ehlers C, Sandman K, Reeve J, Schmitz R. Deletion of the archaeal histone in Methanosarcina mazei Gö1 results in reduced growth and genomic transcription. Mol Microbiol. 2008;67:662-71 pubmed
    HMm is the only archaeal histone in Methanosarcina mazei Göl and recombinant HMm, synthesized by expression of MM1825 in Escherichia coli, has been purified and confirmed to have the DNA binding and compaction properties characteristic ..
  8. Sch fer I, Bailer S, D ser M, B rsch M, Bernal R, Stock D, et al. Crystal structure of the archaeal A1Ao ATP synthase subunit B from Methanosarcina mazei Gö1: Implications of nucleotide-binding differences in the major A1Ao subunits A and B. J Mol Biol. 2006;358:725-40 pubmed publisher
    ..The chimeric nature of the A1Ao ATP synthase from the archaeon Methanosarcina mazei Gö1 was investigated in terms of nucleotide interaction...
  9. Gruber G, Svergun D, Coskun U, Lemker T, Koch M, Schagger H, et al. Structural Insights into the A1 ATPase from the archaeon, Methanosarcina mazei Gö1. Biochemistry. 2001;40:1890-6 pubmed
    The low-resolution structure and overall dimensions of the A(3)B(3)CDF complex of the A(1) ATPase from Methanosarcina mazei Gö1 in solution is analyzed by synchrotron X-ray small-angle scattering...

More Information

Publications93

  1. Pflüger K, Ehrenreich A, Salmon K, Gunsalus R, Deppenmeier U, Gottschalk G, et al. Identification of genes involved in salt adaptation in the archaeon Methanosarcina mazei Gö1 using genome-wide gene expression profiling. FEMS Microbiol Lett. 2007;277:79-89 pubmed
    b>Methanosarcina mazei is a nonhalophilic methanogen that can adapt to 800 mM NaCl. Microarray studies have been used to examine the effect of elevated salinities on the regulation of gene expression in M. mazei...
  2. Miles R, Iyer P, Ferry J. Site-directed mutational analysis of active site residues in the acetate kinase from Methanosarcina thermophila. J Biol Chem. 2001;276:45059-64 pubmed
    ..The recently determined crystal structure of the Methanosarcina thermophila enzyme identifies it as a member of the sugar kinase/Hsc70/actin superfamily based on the fold and ..
  3. Odoi K, Huang Y, Rezenom Y, Liu W. Nonsense and sense suppression abilities of original and derivative Methanosarcina mazei pyrrolysyl-tRNA synthetase-tRNA(Pyl) pairs in the Escherichia coli BL21(DE3) cell strain. PLoS ONE. 2013;8:e57035 pubmed publisher
    Systematic studies of nonsense and sense suppression of the original and three derivative Methanosarcina mazei PylRS-tRNA(Pyl) pairs and cross recognition between nonsense codons and various tRNA(Pyl) anticodons in the Escherichia coli ..
  4. Rother M, Oelgeschläger E, Metcalf W. Genetic and proteomic analyses of CO utilization by Methanosarcina acetivorans. Arch Microbiol. 2007;188:463-72 pubmed
    b>Methanosarcina acetivorans, a member of the methanogenic archaea, can grow with carbon monoxide (CO) as the sole energy source and generates, unlike other methanogens, substantial amounts of acetate and formate in addition to methane...
  5. Yanagisawa T, Ishii R, Fukunaga R, Kobayashi T, Sakamoto K, Yokoyama S. Multistep engineering of pyrrolysyl-tRNA synthetase to genetically encode N(epsilon)-(o-azidobenzyloxycarbonyl) lysine for site-specific protein modification. Chem Biol. 2008;15:1187-97 pubmed publisher
    ..The AzZLys-containing protein was labeled with a fluorescent probe, by Staudinger ligation...
  6. Qu X, Vavilin V, Mazeas L, Lemunier M, Duquennoi C, He P, et al. Anaerobic biodegradation of cellulosic material: batch experiments and modelling based on isotopic data and focusing on aceticlastic and non-aceticlastic methanogenesis. Waste Manag. 2009;29:1828-37 pubmed publisher
    ..and Methanosarcina sp., consuming both acetate and H(2)/H(2)CO(3) as well as acetate-oxidizing syntrophs, were considered...
  7. Robbins J, Murphy M, White B, Mackie R, Ha T, Cann I. Functional analysis of multiple single-stranded DNA-binding proteins from Methanosarcina acetivorans and their effects on DNA synthesis by DNA polymerase BI. J Biol Chem. 2004;279:6315-26 pubmed
    ..and characterization of multiple replication protein A homologs, RPA1, RPA2, and RPA3, from the archaeon Methanosarcina acetivorans...
  8. Guss A, Kulkarni G, Metcalf W. Differences in hydrogenase gene expression between Methanosarcina acetivorans and Methanosarcina barkeri. J Bacteriol. 2009;191:2826-33 pubmed publisher
    b>Methanosarcina acetivorans C2A encodes three putative hydrogenases, including one cofactor F(420)-linked (frh) and two methanophenazine-linked (vht) enzymes...
  9. Ramanujam V, Chary K, Ainavarapu S. Iterative cloning, overexpression, purification and isotopic labeling of an engineered dimer of a Ca(2+)-binding protein of the ??-crystallin superfamily from Methanosarcina acetivorans. Protein Expr Purif. 2012;84:116-22 pubmed publisher
    ..closest member to the family of lens ??-crystallins has been described, for the first time, from the archaea Methanosarcina acetivorans, which is named as M-crystallin. Unlike lens ??-crystallins, M-crystallin exits as a monomer...
  10. Li L, Li Q, Rohlin L, Kim U, Salmon K, Rejtar T, et al. Quantitative proteomic and microarray analysis of the archaeon Methanosarcina acetivorans grown with acetate versus methanol. J Proteome Res. 2007;6:759-71 pubmed
    b>Methanosarcina acetivorans strain C2A is an acetate- and methanol-utilizing methane-producing organism for which the genome, the largest yet sequenced among the Archaea, reveals extensive physiological diversity...
  11. Primak Y, Du M, Miller M, Wells D, Nielsen A, Weyler W, et al. Characterization of a feedback-resistant mevalonate kinase from the archaeon Methanosarcina mazei. Appl Environ Microbiol. 2011;77:7772-8 pubmed publisher
    ..we report the purification, kinetic characterization, and inhibition analysis of the MVK from the archaeon Methanosarcina mazei. The inhibition of the M...
  12. Ding Y, Zhang S, Tomb J, Ferry J. Genomic and proteomic analyses reveal multiple homologs of genes encoding enzymes of the methanol:coenzyme M methyltransferase system that are differentially expressed in methanol- and acetate-grown Methanosarcina thermophila. FEMS Microbiol Lett. 2002;215:127-32 pubmed
    Each of the genomic sequences of Methanosarcina acetivorans, Methanosarcina mazei, and Methanosarcina thermophila revealed two homologs of mtaA, three homologs of mtaB, and three homologs of mtaC encoding enzymes specific for ..
  13. Deppenmeier U, Blaut M, Lentes S, Herzberg C, Gottschalk G. Analysis of the vhoGAC and vhtGAC operons from Methanosarcina mazei strain Gö1, both encoding a membrane-bound hydrogenase and a cytochrome b. Eur J Biochem. 1995;227:261-9 pubmed
    DNA encompassing the structural genes of two membrane-bound hydrogenases from Methanosarcina mazei Gö1 was cloned and sequenced...
  14. Osumi N, Kakehashi Y, Matsumoto S, Nagaoka K, Sakai J, Miyashita K, et al. Identification of the gene for disaggregatase from Methanosarcina mazei. Archaea. 2008;2:185-91 pubmed
    The gene sequences encoding disaggregatase (Dag), the enzyme responsible for dispersion of cell aggregates of Methanosarcina mazei to single cells, were determined for three strains of M. mazei (S-6(T), LYC and TMA)...
  15. Wagner D, Schirmack J, Ganzert L, Morozova D, Mangelsdorf K. Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic archaeon from a Siberian permafrost-affected soil. Int J Syst Evol Microbiol. 2013;63:2986-91 pubmed publisher
    ..The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of Methanosarcina mazei DSM 2053(T) (similarity 99.9 %) and Methanosarcina horonobensis HB-1(T) (similarity 98.7 %)...
  16. Chen Y, Lin Y, Yoshinaga A, Chhotani B, Lorenzini J, Crofts A, et al. Molecular analyses of a three-subunit euryarchaeal clamp loader complex from Methanosarcina acetivorans. J Bacteriol. 2009;191:6539-49 pubmed publisher
    ..Here we use biochemical and mutational analyses to study the structure/function of the Methanosarcina acetivorans clamp loader or replication factor C (RFC) homolog. M...
  17. Veit K, Ehlers C, Ehrenreich A, Salmon K, Hovey R, Gunsalus R, et al. Global transcriptional analysis of Methanosarcina mazei strain Gö1 under different nitrogen availabilities. Mol Genet Genomics. 2006;276:41-55 pubmed
    ..To address this on a genome-wide scale, transcriptional analysis was performed on the model methanogen Methanosarcina mazei strain Gö1 using DNA-microarrays...
  18. Maupin Furlow J, Ferry J. Characterization of the cdhD and cdhE genes encoding subunits of the corrinoid/iron-sulfur enzyme of the CO dehydrogenase complex from Methanosarcina thermophila. J Bacteriol. 1996;178:340-6 pubmed
    The CO dehydrogenase enzyme complex from Methanosarcina thermophila contains a corrinoid/iron-sulfur enzyme composed of two subunits (delta and gamma)...
  19. Lin L, Yoshinaga A, Lin Y, Guzman C, Chen Y, Mei S, et al. Molecular analyses of an unusual translesion DNA polymerase from Methanosarcina acetivorans C2A. J Mol Biol. 2010;397:13-30 pubmed publisher
    ..MacDinB-1, the homolog from the euryarchaeon Methanosarcina acetivorans that is characterized in this study, belongs to cluster II...
  20. Lawrence S, Luther K, Schindelin H, Ferry J. Structural and functional studies suggest a catalytic mechanism for the phosphotransacetylase from Methanosarcina thermophila. J Bacteriol. 2006;188:1143-54 pubmed publisher
    ..Two crystal structures of phosphotransacetylase from the methanogenic archaeon Methanosarcina thermophila in complex with the substrate CoA revealed one CoA (CoA1) bound in the proposed active site cleft ..
  21. Roessler M, Pfl ger K, Flach H, Lienard T, Gottschalk G, M ller V. Identification of a salt-induced primary transporter for glycine betaine in the methanogen Methanosarcina mazei Gö1. Appl Environ Microbiol. 2002;68:2133-9 pubmed
    The salt adaptation of the methanogenic archaeon Methanosarcina mazei Gö1 was studied at the physiological and molecular levels. The freshwater organism M...
  22. Kitabatake M, So M, Tumbula D, S ll D. Cysteine biosynthesis pathway in the archaeon Methanosarcina barkeri encoded by acquired bacterial genes?. J Bacteriol. 2000;182:143-5 pubmed
    ..Complementation of a cysteine auxotrophic Escherichia coli strain NK3 led to the isolation of the Methanosarcina barkeri cysK gene [encoding O-acetylserine (thiol)-lyase-A], which displays great similarity to bacterial cysK ..
  23. Ascenzi P, Pesce A, Nardini M, Bolognesi M, Ciaccio C, Coletta M, et al. Reductive nitrosylation of Methanosarcina acetivorans protoglobin: a comparative study. Biochem Biophys Res Commun. 2013;430:1301-5 pubmed publisher
    b>Methanosarcina acetivorans is a strictly anaerobic non-motile methane-producing Archaea expressing protoglobin (Pgb) which might either facilitate O(2) detoxification or act as a CO sensor/supplier in methanogenesis. Unusually, M...
  24. Nickel L, Weidenbach K, Jäger D, Backofen R, Lange S, Heidrich N, et al. Two CRISPR-Cas systems in Methanosarcina mazei strain Gö1 display common processing features despite belonging to different types I and III. RNA Biol. 2013;10:779-91 pubmed publisher
    ..We analyzed the two CRISPR-Cas systems in Methanosarcina mazei strain Gö1...
  25. Bose A, Pritchett M, Rother M, Metcalf W. Differential regulation of the three methanol methyltransferase isozymes in Methanosarcina acetivorans C2A. J Bacteriol. 2006;188:7274-83 pubmed
    Genetic analysis of the three methanol-specific methyltransferase 1 operons (mtaCB1, mtaCB2, and mtaCB3) in Methanosarcina acetivorans led to the suggestion that each of them has a discrete function during growth on methanol, which might ..
  26. Chen Y, Apolinario E, Brachova L, Kelman Z, Li Z, Nikolau B, et al. A nuclear magnetic resonance based approach to accurate functional annotation of putative enzymes in the methanogen Methanosarcina acetivorans. BMC Genomics. 2011;12 Suppl 1:S7 pubmed publisher
    ..Here, we describe the use of nuclear magnetic resonance (NMR)-based ligand screening as a tool for testing functional assignments of putative enzymes that may be of variable reliability...
  27. Opulencia R, Bose A, Metcalf W. Physiology and posttranscriptional regulation of methanol:coenzyme M methyltransferase isozymes in Methanosarcina acetivorans C2A. J Bacteriol. 2009;191:6928-35 pubmed publisher
    b>Methanosarcina species possess three operons (mtaCB1, mtaCB2, and mtaCB3) encoding methanol-specific methyltransferase 1 (MT1) isozymes and two genes (mtaA1 and mtaA2) with the potential to encode a methanol-specific methyltransferase 2 (..
  28. Gencic S, Duin E, Grahame D. Tight coupling of partial reactions in the acetyl-CoA decarbonylase/synthase (ACDS) multienzyme complex from Methanosarcina thermophila: acetyl C-C bond fragmentation at the a cluster promoted by protein conformational changes. J Biol Chem. 2010;285:15450-63 pubmed publisher
    ..tight coupling was investigated by analysis of three recombinant A cluster proteins, ACDS beta subunit from Methanosarcina thermophila, acetyl-CoA synthase of Carboxydothermus hydrogenoformans (ACS(Ch)), and truncated ACS(Ch) lacking ..
  29. Spanheimer R, Muller V. The molecular basis of salt adaptation in Methanosarcina mazei Gö1. Arch Microbiol. 2008;190:271-9 pubmed publisher
    ..classical biochemistry shed light on the processes conferring salt adaptation in the methanogenic archaeon Methanosarcina mazei Gö1...
  30. Weidenbach K, Ehlers C, Kock J, Schmitz R. NrpRII mediates contacts between NrpRI and general transcription factors in the archaeon Methanosarcina mazei Gö1. FEBS J. 2010;277:4398-411 pubmed publisher
    We report here on the formation of a complex between the two NrpR homologs present in Methanosarcina mazei Gö1 and their binding properties to the nifH and glnK(1) promoters...
  31. Schlegel K, Welte C, Deppenmeier U, Muller V. Electron transport during aceticlastic methanogenesis by Methanosarcina acetivorans involves a sodium-translocating Rnf complex. FEBS J. 2012;279:4444-52 pubmed publisher
    The anaerobic methanogenic archaeon Methanosarcina acetivorans lives under extreme energy limitation. Methanogenesis from acetate as carried out by M...
  32. Lira Silva E, Santiago Martínez M, García Contreras R, Zepeda Rodriguez A, Marin Hernandez A, Moreno Sanchez R, et al. Cd2+ resistance mechanisms in Methanosarcina acetivorans involve the increase in the coenzyme M content and induction of biofilm synthesis. Environ Microbiol Rep. 2013;5:799-808 pubmed publisher
    To assess what defence mechanisms are triggered by Cd(2+) stress in Methanosarcina acetivorans, cells were cultured at different cadmium concentrations...
  33. Conway De Macario E, Dugan C, Macario A. Identification of a grpE heat-shock gene homolog in the archaeon Methanosarcina mazei. J Mol Biol. 1994;240:95-101 pubmed
    A grpE heat-shock gene was found by sequencing in the genome of the methanogenic archaeon Methanosarcina mazei S-6. It is the first example of grpE from the phylogenetic domain Archaea...
  34. Spanheimer R, Hoffmann M, K gl S, Schmidt S, Pfl ger K, M ller V. Differential regulation of Ota and Otb, two primary glycine betaine transporters in the methanogenic archaeon methanosarcina mazei Gö1. J Mol Microbiol Biotechnol. 2008;15:255-63 pubmed publisher
    ..b>Methanosarcina mazei differs from most other methanoarchaea in having two gene clusters both encoding a potential glycine ..
  35. Lienard T, Becher B, Marschall M, Bowien S, Gottschalk G. Sodium ion translocation by N5-methyltetrahydromethanopterin: coenzyme M methyltransferase from Methanosarcina mazei Gö1 reconstituted in ether lipid liposomes. Eur J Biochem. 1996;239:857-64 pubmed
    ..The enzyme was purified from acetate-grown Methanosarcina mazei Gö1 by a two-step solubilization with n-octyl-beta-glucoside, chromatography on hydroxyapatite, and by ..
  36. Reichlen M, Murakami K, Ferry J. Functional analysis of the three TATA binding protein homologs in Methanosarcina acetivorans. J Bacteriol. 2010;192:1511-7 pubmed publisher
    The roles of three TATA binding protein (TBP) homologs (TBP1, TBP2, and TBP3) in the archaeon Methanosarcina acetivorans were investigated by using genetic and molecular approaches...
  37. Mayerhofer L, Conway De Macario E, Yao R, Macario A. Structure, organization, and expression of genes coding for envelope components in the archaeon Methanosarcina mazei S-6. Arch Microbiol. 1998;169:339-45 pubmed
    ..We began the dissection of the antigenic mosaic of the methanogen Methanosarcina mazei S-6 by gene cloning and sequencing...
  38. Borup B, Ferry J. Cysteine biosynthesis in the Archaea: Methanosarcina thermophila utilizes O-acetylserine sulfhydrylase. FEMS Microbiol Lett. 2000;189:205-10 pubmed
    ..Enzyme activities in extracts of Methanosarcina thermophila grown with combinations of cysteine and sulfide as sulfur sources indicated that this archaeon ..
  39. Zhang J, White A, Kuettner H, Boccazzi P, Metcalf W. Directed mutagenesis and plasmid-based complementation in the methanogenic archaeon Methanosarcina acetivorans C2A demonstrated by genetic analysis of proline biosynthesis. J Bacteriol. 2002;184:1449-54 pubmed
    We report here the first use of directed mutagenesis in Methanosarcina acetivorans C2A...
  40. Bose A, Kulkarni G, Metcalf W. Regulation of putative methyl-sulphide methyltransferases in Methanosarcina acetivorans C2A. Mol Microbiol. 2009;74:227-238 pubmed publisher
    The regulation of the Methanosarcina acetivorans mtsD, mtsF and mtsH genes, which encode putative corrinoid/methyltransferase isozymes involved in methylsulphide metabolism, was examined by a variety of methods, suggesting that their ..
  41. Hauenstein S, Perona J. Redundant synthesis of cysteinyl-tRNACys in Methanosarcina mazei. J Biol Chem. 2008;283:22007-17 pubmed publisher
    ..Here, we explore the function of all three enzymes in the mesophilic methanogen Methanosarcina mazei. The genome of M...
  42. Pisa K, Weidner C, Maischak H, Kavermann H, Muller V. The coupling ion in the methanoarchaeal ATP synthases: H(+) vs. Na(+) in the A(1)A(o) ATP synthase from the archaeon Methanosarcina mazei Gö1. FEMS Microbiol Lett. 2007;277:56-63 pubmed
    ..to address the nature of the coupling ion, the operon encoding the A(1)A(o) ATP synthase from the mesophile Methanosarcina mazei Gö1 was cloned in an expression vector and it was expressed in the F(1)F(o) ATP synthase-negative ..
  43. Shimizu S, Upadhye R, Ishijima Y, Naganuma T. Methanosarcina horonobensis sp. nov., a methanogenic archaeon isolated from a deep subsurface Miocene formation. Int J Syst Evol Microbiol. 2011;61:2503-7 pubmed publisher
    ..4 mol%. 16S rRNA gene sequencing revealed that the strain was a member of the genus Methanosarcina but that it clearly differed from all recognized species of this genus (93.1-97.9?% sequence similarity)...
  44. Jasso Ch vez R, Apolinario E, Sowers K, Ferry J. MrpA functions in energy conversion during acetate-dependent growth of Methanosarcina acetivorans. J Bacteriol. 2013;195:3987-94 pubmed publisher
    The role of the multisubunit sodium/proton antiporter (Mrp) of Methanosarcina acetivorans was investigated with a mutant deleted for the gene encoding the MrpA subunit...
  45. Matschiavelli N, Oelgeschläger E, Cocchiararo B, Finke J, Rother M. Function and regulation of isoforms of carbon monoxide dehydrogenase/acetyl coenzyme A synthase in Methanosarcina acetivorans. J Bacteriol. 2012;194:5377-87 pubmed publisher
    ..b>Methanosarcina acetivorans C2A contains two very similar copies of a six-gene operon (cdh genes) encoding two isoforms of ..
  46. Lessner D, Ferry J. The archaeon Methanosarcina acetivorans contains a protein disulfide reductase with an iron-sulfur cluster. J Bacteriol. 2007;189:7475-84 pubmed publisher
    b>Methanosarcina acetivorans, a strictly anaerobic methane-producing species belonging to the domain Archaea, contains a gene cluster annotated with homologs encoding oxidative stress proteins...
  47. Rother M, Sattler C, Stock T. Studying gene regulation in methanogenic archaea. Methods Enzymol. 2011;494:91-110 pubmed publisher
    ..The major models for studying the biology of methanogens are members of the Methanococcus and Methanosarcina species...
  48. Lessner D, Li L, Li Q, Rejtar T, Andreev V, Reichlen M, et al. An unconventional pathway for reduction of CO2 to methane in CO-grown Methanosarcina acetivorans revealed by proteomics. Proc Natl Acad Sci U S A. 2006;103:17921-6 pubmed
    b>Methanosarcina acetivorans produces acetate, formate, and methane when cultured with CO as the growth substrate [Rother M, Metcalf WW (2004) Proc Natl Acad Sci USA 101:], which suggests novel features of CO metabolism...
  49. Funk T, Gu W, Friedrich S, Wang H, Gencic S, Grahame D, et al. Chemically distinct Ni sites in the A-cluster in subunit beta of the acetyl-CoA decarbonylase/synthase complex from Methanosarcina thermophila: Ni L-edge absorption and X-ray magnetic circular dichroism analyses. J Am Chem Soc. 2004;126:88-95 pubmed
    ..The properties of Ni in the active site A-cluster in the ACDS beta subunit from Methanosarcina thermophila were investigated...
  50. Bose A, Pritchett M, Metcalf W. Genetic analysis of the methanol- and methylamine-specific methyltransferase 2 genes of Methanosarcina acetivorans C2A. J Bacteriol. 2008;190:4017-26 pubmed publisher
    ..The mtaA1, mtaA2, and mtbA genes of Methanosarcina acetivorans C2A encode putative methanol- or methylamine-specific MT2 enzymes...
  51. Chen Y, Kocherginskaya S, Lin Y, Sriratana B, Lagunas A, Robbins J, et al. Biochemical and mutational analyses of a unique clamp loader complex in the archaeon Methanosarcina acetivorans. J Biol Chem. 2005;280:41852-63 pubmed
    ..Here, we describe a new form of clamp loader from the archaeon Methanosarcina acetivorans...
  52. Fournier G, Gogarten J. Evolution of acetoclastic methanogenesis in Methanosarcina via horizontal gene transfer from cellulolytic Clostridia. J Bacteriol. 2008;190:1124-7 pubmed
    ..genes required for acetoclastic methanogenesis, ackA and pta, were horizontally transferred to the ancestor of Methanosarcina from a derived cellulolytic organism in the class Clostridia...
  53. Lin Y, Guzman C, McKinney M, Nair S, Ha T, Cann I. Methanosarcina acetivorans flap endonuclease 1 activity is inhibited by a cognate single-stranded-DNA-binding protein. J Bacteriol. 2006;188:6153-67 pubmed
    ..Thus, the archaeal protein is called replication protein A (RPA), as in eukaryotes. Unlike most organisms, Methanosarcina acetivorans harbors multiple functional RPA proteins, and it was our interest to determine whether the ..
  54. Jäger D, Sharma C, Thomsen J, Ehlers C, Vogel J, Schmitz R. Deep sequencing analysis of the Methanosarcina mazei Gö1 transcriptome in response to nitrogen availability. Proc Natl Acad Sci U S A. 2009;106:21878-82 pubmed publisher
    b>Methanosarcina mazei and related mesophilic archaea are the only organisms fermenting acetate, methylamines, and methanol to methane and carbon dioxide, contributing significantly to greenhouse gas production...
  55. De Vrieze J, Hennebel T, Boon N, Verstraete W. Methanosarcina: the rediscovered methanogen for heavy duty biomethanation. Bioresour Technol. 2012;112:1-9 pubmed publisher
    ..In the latter configuration Methanosarcina species appear to be of crucial importance. Methanosarcina sp...
  56. Zmijewski M, Sk rko Glonek J, Tanfani F, Banecki B, Kotlarz A, Macario A, et al. The DnaK chaperones from the archaeon Methanosarcina mazei and the bacterium Escherichia coli have different substrate specificities. Acta Biochim Pol. 2007;54:509-22 pubmed
    ..In a previous work we demonstrated that DnaK from the archaeon Methanosarcina mazei (DnaK(Mm)) and the DnaK from the bacterium Escherichia coli (DnaK(Ec)) were functionally similar when ..
  57. Galagan J, Nusbaum C, Roy A, Endrizzi M, Macdonald P, FitzHugh W, et al. The genome of M. acetivorans reveals extensive metabolic and physiological diversity. Genome Res. 2002;12:532-42 pubmed
    ..Here we report the complete genome sequence of an acetate-utilizing methanogen, Methanosarcina acetivorans C2A...
  58. De Biase A, Macario A, Conway de Macario E. Effect of heat stress on promoter binding by transcription factors in the cytosol of the archaeon Methanosarcina mazeii. Gene. 2002;282:189-97 pubmed
    ..and regulation of the stress genes in the hsp70(dnaK) locus of the mesophilic, methanogenic archaeon Methanosarcina mazeii...
  59. Zayas C, Woodson J, Escalante Semerena J. The cobZ gene of Methanosarcina mazei Go1 encodes the nonorthologous replacement of the alpha-ribazole-5'-phosphate phosphatase (CobC) enzyme of Salmonella enterica. J Bacteriol. 2006;188:2740-3 pubmed
    Open reading frame (ORF) Mm2058 of the methanogenic archaeon Methanosarcina mazei strain Gö1 was shown in vivo and in vitro to encode the nonorthologous replacement of the alpha-ribazole-phosphate phosphatase (CobC; EC 3.1.3...
  60. Ciaccio C, Pesce A, Tundo G, Tilleman L, Bertolacci L, Dewilde S, et al. Functional and structural roles of the N-terminal extension in Methanosarcina acetivorans protoglobin. Biochim Biophys Acta. 2013;1834:1813-23 pubmed publisher
    Functional and structural properties of protoglobin from Methanosarcina acetivorans, whose Cys(101)E20 residue was mutated to Ser (MaPgb*), and of mutants missing either the first 20 N-terminal amino acids (MaPgb*-?N20 mutant), or the ..
  61. Gorrell A, Ferry J. Investigation of the Methanosarcina thermophila acetate kinase mechanism by fluorescence quenching. Biochemistry. 2007;46:14170-6 pubmed
    ..A catalytic mechanism for the enzyme from Methanosarcina thermophila has been proposed on the basis of the crystal structure and kinetic analyses of amino acid ..
  62. Ferry J, Lessner D. Methanogenesis in marine sediments. Ann N Y Acad Sci. 2008;1125:147-57 pubmed publisher
    ..Recent molecular, biochemical, bioinformatic, proteomic, and microarray analyses of the marine isolate Methanosarcina acetivorans has revealed that the pathway for acetate conversion to methane differs significantly from that in ..
  63. Satish Kumar V, Ferry J, Maranas C. Metabolic reconstruction of the archaeon methanogen Methanosarcina Acetivorans. BMC Syst Biol. 2011;5:28 pubmed publisher
    ..b>Methanosarcina acetivorans, with a genome size of ~5...
  64. Nardini M, Pesce A, Thijs L, Saito J, Dewilde S, Alam M, et al. Archaeal protoglobin structure indicates new ligand diffusion paths and modulation of haem-reactivity. EMBO Rep. 2008;9:157-63 pubmed publisher
    ..Protoglobin from Methanosarcina acetivorans C2A-a strictly anaerobic methanogenic Archaea-is, to the best of our knowledge, the latest entry ..
  65. Burke S, Krzycki J. Reconstitution of Monomethylamine:Coenzyme M methyl transfer with a corrinoid protein and two methyltransferases purified from Methanosarcina barkeri. J Biol Chem. 1997;272:16570-7 pubmed
    ..MMAMT functions as a MMA:MMCP methyltransferase, while MT2-A functions as a methyl-MMCP:CoM methyltransferase...
  66. Pesce A, Tilleman L, Dewilde S, Ascenzi P, Coletta M, Ciaccio C, et al. Structural heterogeneity and ligand gating in ferric Methanosarcina acetivorans protoglobin mutants. IUBMB Life. 2011;63:287-94 pubmed publisher
    Protoglobin from Methanosarcina acetivorans C2A (MaPgb), a strictly anaerobic methanogenic Archaea, displays peculiar structural and functional properties within members of the hemoglobin superfamily...
  67. Rother M, Metcalf W. Anaerobic growth of Methanosarcina acetivorans C2A on carbon monoxide: an unusual way of life for a methanogenic archaeon. Proc Natl Acad Sci U S A. 2004;101:16929-34 pubmed
    ..To further examine the role of hydrogen in this process, we tested the ability of Methanosarcina acetivorans C2A, a metabolically versatile methanogen devoid of significant hydrogen metabolism, to use CO as ..
  68. Sowers K, Thai T, Gunsalus R. Transcriptional regulation of the carbon monoxide dehydrogenase gene (cdhA) in Methanosarcina thermophila. J Biol Chem. 1993;268:23172-8 pubmed
    ..examine the expression of a gene encoding a highly regulated catabolic enzyme from the methanogenic archaea, a Methanosarcina thermophila lambda gt11 chromosomal library was probed with antiserum prepared against the 89-kDa subunit of ..
  69. Borup B, Ferry J. O-Acetylserine sulfhydrylase from Methanosarcina thermophila. J Bacteriol. 2000;182:45-50 pubmed
    ..We report the purification and characterization of OASS from acetate-grown Methanosarcina thermophila, a moderately thermophilic methanoarchaeon...
  70. Benedict M, Gonnerman M, Metcalf W, Price N. Genome-scale metabolic reconstruction and hypothesis testing in the methanogenic archaeon Methanosarcina acetivorans C2A. J Bacteriol. 2012;194:855-65 pubmed publisher
    b>Methanosarcina acetivorans strain C2A is a marine methanogenic archaeon notable for its substrate utilization, genetic tractability, and novel energy conservation mechanisms...
  71. Reichlen M, Vepachedu V, Murakami K, Ferry J. MreA functions in the global regulation of methanogenic pathways in Methanosarcina acetivorans. MBio. 2012;3:e00189-12 pubmed publisher
    ..MA3302 gene locus (designated MreA) previously annotated as a hypothetical protein in the methanogenic species Methanosarcina acetivorans of the domain Archaea...
  72. Pritchett M, Metcalf W. Genetic, physiological and biochemical characterization of multiple methanol methyltransferase isozymes in Methanosarcina acetivorans C2A. Mol Microbiol. 2005;56:1183-94 pubmed publisher
    Biochemical evidence suggests that methanol catabolism in Methanosarcina species requires the concerted effort of methanol:5-hydroxybenzimidazolylcobamide methyltransferase (MtaB), a corrinoid-containing methyl-accepting protein (MtaC) ..
  73. Pritchett M, Zhang J, Metcalf W. Development of a markerless genetic exchange method for Methanosarcina acetivorans C2A and its use in construction of new genetic tools for methanogenic archaea. Appl Environ Microbiol. 2004;70:1425-33 pubmed
    A new genetic technique for constructing mutants of Methanosarcina acetivorans C2A by using hpt as a counterselectable marker was developed...
  74. Franke Whittle I, Goberna M, Pfister V, Insam H. Design and development of the ANAEROCHIP microarray for investigation of methanogenic communities. J Microbiol Methods. 2009;79:279-88 pubmed publisher
    ..signals were obtained for Methanoculleus, and weaker signals, in decreasing order, were obtained for Methanosarcina, Methanobacterium, Methanobrevibacter, and Methanosphaera...
  75. Welte C, Deppenmeier U. Re-evaluation of the function of the F420 dehydrogenase in electron transport of Methanosarcina mazei. FEBS J. 2011;278:1277-87 pubmed publisher
    b>Methanosarcina mazei is a methanogenic archaeon that is able to thrive on various substrates and therefore contains a variety of redox-active proteins involved in both cytoplasmic and membrane-bound electron transport...
  76. Wang M, Tomb J, Ferry J. Electron transport in acetate-grown Methanosarcina acetivorans. BMC Microbiol. 2011;11:165 pubmed publisher
    ..Rhodobacter nitrogen fixation) complexes distributed in the domain Bacteria distinguishes non-H?-utilizing Methanosarcina acetivorans from H?-utilizing species suggesting fundamentally different electron transport pathways...
  77. Ehlers C, Weidenbach K, Veit K, Forchhammer K, Schmitz R. Unique mechanistic features of post-translational regulation of glutamine synthetase activity in Methanosarcina mazei strain Gö1 in response to nitrogen availability. Mol Microbiol. 2005;55:1841-54 pubmed
    ..The GlnK(1) protein of the methanogenic archaeon Methanosarcina mazei strain Go1 interacts and forms stable complexes with glutamine synthetase (GlnA(1))...
  78. Murakami E, Deppenmeier U, Ragsdale S. Characterization of the intramolecular electron transfer pathway from 2-hydroxyphenazine to the heterodisulfide reductase from Methanosarcina thermophila. J Biol Chem. 2001;276:2432-9 pubmed
    ..HDR from Methanosarcina thermophila contains two b-hemes and two [Fe(4)S(4)] clusters...
  79. Yanagisawa T, Ishii R, Fukunaga R, Kobayashi T, Sakamoto K, Yokoyama S. Crystallographic studies on multiple conformational states of active-site loops in pyrrolysyl-tRNA synthetase. J Mol Biol. 2008;378:634-52 pubmed publisher
    ..In the present study, the carboxy-terminal catalytic fragment of Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS) was analyzed by X-ray crystallography and site-directed mutagenesis...
  80. Oelgeschl ger E, Rother M. In vivo role of three fused corrinoid/methyl transfer proteins in Methanosarcina acetivorans. Mol Microbiol. 2009;72:1260-72 pubmed publisher
    b>Methanosarcina acetivorans is able to use carbon monoxide (CO) as the sole source of energy for growth. Its carboxidotrophic growth is peculiar as it involves formation of acetate, formate and methylated thiols, besides methane...
  81. Baumer S, Ide T, Jacobi C, Johann A, Gottschalk G, Deppenmeier U. The F420H2 dehydrogenase from Methanosarcina mazei is a Redox-driven proton pump closely related to NADH dehydrogenases. J Biol Chem. 2000;275:17968-73 pubmed publisher
    ..dehydrogenase is part of the energy conserving electron transport system of the methanogenic archaeon Methanosarcina mazei Gö1...
  82. Bizukojc M, Dietz D, Sun J, Zeng A. Metabolic modelling of syntrophic-like growth of a 1,3-propanediol producer, Clostridium butyricum, and a methanogenic archeon, Methanosarcina mazei, under anaerobic conditions. Bioprocess Biosyst Eng. 2010;33:507-23 pubmed publisher
    ..We have proposed a novel mixed culture comprising C. butyricum and a methane bacterium, Methanosarcina mazei, to relieve the inhibition and to utilise the by-products for energy production...
  83. Bikiel D, Forti F, Boechi L, Nardini M, Luque F, Marti M, et al. Role of heme distortion on oxygen affinity in heme proteins: the protoglobin case. J Phys Chem B. 2010;114:8536-43 pubmed publisher
    ..These findings shed light into the peculiar binding affinity of Methanosarcina acetivorans protoglobin, a protein that contains a highly distorted heme...
  84. Gencic S, Grahame D. Two separate one-electron steps in the reductive activation of the A cluster in subunit beta of the ACDS complex in Methanosarcina thermophila. Biochemistry. 2008;47:5544-55 pubmed publisher