methanobacterium

Summary

Summary: A genus of anaerobic, rod-shaped METHANOBACTERIACEAE. Its organisms are nonmotile and use ammonia as the sole source of nitrogen. These methanogens are found in aquatic sediments, soil, sewage, and the gastrointestinal tract of animals.

Top Publications

  1. Yu X, VanLoock M, Poplawski A, Kelman Z, Xiang T, Tye B, et al. The Methanobacterium thermoautotrophicum MCM protein can form heptameric rings. EMBO Rep. 2002;3:792-7 pubmed
    ..In most archaea a single MCM protein exists. The protein from Methanobacterium thermoautotrophicum (mtMCM) has been reported to assemble into a large complex consistent with a dodecamer...
  2. Zhelkovsky A, McReynolds L. Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase - engineering a thermostable ATP independent enzyme. BMC Mol Biol. 2012;13:24 pubmed publisher
    ..with these desirable properties we performed mutational analysis of the archaeal thermophilic RNA ligase from Methanobacterium thermoautotrophicum...
  3. Back J, Chung J, Park Y, Kim K, Han Y. Endonuclease IV enhances base excision repair of endonuclease III from Methanobacterium thermoautotrophicum. DNA Repair (Amst). 2003;2:455-70 pubmed
    ..We expressed and characterized two putative endonuclease genes from Methanobacterium thermoautotrophicum, Mt0764 and Mt1010, encoding homologues of endonuclease III (endo III) and endonuclease ..
  4. Fletcher R, Shen J, Gómez Llorente Y, Martín C, Carazo J, Chen X. Double hexamer disruption and biochemical activities of Methanobacterium thermoautotrophicum MCM. J Biol Chem. 2005;280:42405-10 pubmed
    b>Methanobacterium thermoautotrophicum MCM (mtMCM) is a helicase required for DNA replication. Previous electron microscopy studies have shown mtMCM in several oligomeric forms...
  5. N lling J, Pihl T, Reeve J. Cloning, sequencing, and growth phase-dependent transcription of the coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase-encoding genes from Methanobacterium thermoautotrophicum delta H and Methanopyrus kandleri. J Bacteriol. 1995;177:7238-44 pubmed
    ..reductases (CH2 = H4MPT reductases), and their flanking regions have been cloned from Methanobacterium thermoautotrophicum delta H and Methanopyrus kandleri and sequenced...
  6. Christendat D, Saridakis V, Dharamsi A, Bochkarev A, Pai E, Arrowsmith C, et al. Crystal structure of dTDP-4-keto-6-deoxy-D-hexulose 3,5-epimerase from Methanobacterium thermoautotrophicum complexed with dTDP. J Biol Chem. 2000;275:24608-12 pubmed publisher
    ..The crystal structure of RmlC from Methanobacterium thermoautotrophicum was determined in the presence and absence of dTDP, a substrate analogue...
  7. Pihl T, Sharma S, Reeve J. Growth phase-dependent transcription of the genes that encode the two methyl coenzyme M reductase isoenzymes and N5-methyltetrahydromethanopterin:coenzyme M methyltransferase in Methanobacterium thermoautotrophicum delta H. J Bacteriol. 1994;176:6384-91 pubmed
    The genes encoding the two isoenzymes of methyl coenzyme M reductase (MRI and MRII) in Methanobacterium thermoautotrophicum delta H have been cloned and sequenced...
  8. Hedderich R, Koch J, Linder D, Thauer R. The heterodisulfide reductase from Methanobacterium thermoautotrophicum contains sequence motifs characteristic of pyridine-nucleotide-dependent thioredoxin reductases. Eur J Biochem. 1994;225:253-61 pubmed
    ..46 kDa) did not show sequence similarity to other known proteins, but appears to possess a C-terminal hydrophobic alpha-helix that might function as a membrane anchor. Although hdrB and hdrC are juxtaposed, these genes are not near hdrA...
  9. Kelman Z, Pietrokovski S, Hurwitz J. Isolation and characterization of a split B-type DNA polymerase from the archaeon Methanobacterium thermoautotrophicum deltaH. J Biol Chem. 1999;274:28751-61 pubmed
    We describe here the isolation and characterization of a B-type DNA polymerase (PolB) from the archaeon Methanobacterium thermoautotrophicum DeltaH. Uniquely, the catalytic domains of M...

More Information

Publications76

  1. Torchia C, Takagi Y, Ho C. Archaeal RNA ligase is a homodimeric protein that catalyzes intramolecular ligation of single-stranded RNA and DNA. Nucleic Acids Res. 2008;36:6218-27 pubmed publisher
    ..Here, we report the characterization of an RNA ligase from the thermophilic archaeon, Methanobacterium thermoautotrophicum...
  2. Saridakis V, Christendat D, Kimber M, Dharamsi A, Edwards A, Pai E. Insights into ligand binding and catalysis of a central step in NAD+ synthesis: structures of Methanobacterium thermoautotrophicum NMN adenylyltransferase complexes. J Biol Chem. 2001;276:7225-32 pubmed publisher
    ..The structure of Methanobacterium thermoautotrophicum NMNATase determined using multiwavelength anomalous dispersion phasing revealed a ..
  3. Zhelkovsky A, McReynolds L. Simple and efficient synthesis of 5' pre-adenylated DNA using thermostable RNA ligase. Nucleic Acids Res. 2011;39:e117 pubmed publisher
    ..of pre-adenylated DNA linkers/adapters for next-generation sequencing using thermostable RNA ligase from Methanobacterium thermoautotrophicum (MthRnl)...
  4. Parfenova L, Crane B, Rothberg B. Modulation of MthK potassium channel activity at the intracellular entrance to the pore. J Biol Chem. 2006;281:21131-8 pubmed
    ..with the LB2003 K(+) uptake-deficient strain of Escherichia coli to analyze residues that are critical to Methanobacterium thermoautotrophicum potassium channel (MthK) function...
  5. Mukhopadhyay B, Stoddard S, Wolfe R. Purification, regulation, and molecular and biochemical characterization of pyruvate carboxylase from Methanobacterium thermoautotrophicum strain deltaH. J Biol Chem. 1998;273:5155-66 pubmed
    We discovered that Methanobacterium thermoautotrophicum strain DeltaH possessed pyruvate carboxylase (PYC), and this biotin prototroph required exogenously supplied biotin to exhibit detectable amounts of PYC activity...
  6. Soderberg T, Chen A, Poulter C. Geranylgeranylglyceryl phosphate synthase. Characterization of the recombinant enzyme from Methanobacterium thermoautotrophicum. Biochemistry. 2001;40:14847-54 pubmed
    ..The gene encoding GGGP synthase from Methanobacterium thermoautotrophicum was cloned using probes designed from the N-terminal sequence determined from the ..
  7. Borrel G, Joblin K, Guedon A, Colombet J, Tardy V, Lehours A, et al. Methanobacterium lacus sp. nov., isolated from the profundal sediment of a freshwater meromictic lake. Int J Syst Evol Microbiol. 2012;62:1625-9 pubmed publisher
    ..phylogenetic analyses based on 16S rRNA gene sequences, strain 17A1(T) appeared to be a member of the genus Methanobacterium, with Methanobacterium beijingense 8-2(T) (96...
  8. Braks I, Hoppert M, Roge S, Mayer F. Structural aspects and immunolocalization of the F420-reducing and non-F420-reducing hydrogenases from Methanobacterium thermoautotrophicum Marburg. J Bacteriol. 1994;176:7677-87 pubmed
    ..12.99.1.) were isolated from a crude extract of Methanobacterium thermoautotrophicum Marburg...
  9. Men Y, Feil H, Verberkmoes N, Shah M, Johnson D, Lee P, et al. Sustainable syntrophic growth of Dehalococcoides ethenogenes strain 195 with Desulfovibrio vulgaris Hildenborough and Methanobacterium congolense: global transcriptomic and proteomic analyses. ISME J. 2012;6:410-21 pubmed publisher
    ..vulgaris Hildenborough (DVH) as a co-culture, as well as with DVH and the hydrogenotrophic methanogen Methanobacterium congolense (MC) as a tri-culture using lactate as the sole energy and carbon source...
  10. Amegbey G, Stothard P, Kuznetsova E, Yee A, Arrowsmith C, Wishart D. Solution Structure of MTH0776 from Methanobacterium thermoautotrophicum. J Biomol NMR. 2005;33:51-6 pubmed publisher
  11. Gorkovenko A, Roberts M. Cyclic 2,3-diphosphoglycerate as a component of a new branch in gluconeogenesis in Methanobacterium thermoautotrophicum delta H. J Bacteriol. 1993;175:4087-95 pubmed
    A unique compound, cyclic 2,3-diphosphoglycerate (cDPG), is the major soluble carbon and phosphorus solute in Methanobacterium thermoautotrophicum delta H under optimal conditions of cell growth...
  12. Karadagli F, Rittmann B. A mathematical model for the kinetics of Methanobacterium bryantii M.o.H. considering hydrogen thresholds. Biodegradation. 2007;18:453-64 pubmed
    ..such as substrate thresholds and survival mode observed in experiments with the H2-oxidizing methanogen Methanobacterium bryantii M.o.H. We apply our model to the experimental data presented in our companion paper on H2 thresholds...
  13. Hochheimer A, Schmitz R, Thauer R, Hedderich R. The tungsten formylmethanofuran dehydrogenase from Methanobacterium thermoautotrophicum contains sequence motifs characteristic for enzymes containing molybdopterin dinucleotide. Eur J Biochem. 1995;234:910-20 pubmed
    ..We report here on the primary structures of the four subunits FwdABCD of the tungsten enzyme from Methanobacterium thermoautotrophicum which were determined by cloning and sequencing the encoding genes fwdABCD...
  14. Vaupel M, Dietz H, Linder D, Thauer R. Primary structure of cyclohydrolase (Mch) from Methanobacterium thermoautotrophicum (strain Marburg) and functional expression of the mch gene in Escherichia coli. Eur J Biochem. 1996;236:294-300 pubmed
    ..The mch gene was overexpressed in Escherichia coli yielding an active enzyme of 37 kDa with a specific activity of 30 U/mg cell extract protein...
  15. Wu B, Yee A, Huang Y, Ramelot T, Cort J, Semesi A, et al. The solution structure of ribosomal protein S17E from Methanobacterium thermoautotrophicum: a structural homolog of the FF domain. Protein Sci. 2008;17:583-8 pubmed publisher
    The ribosomal protein S17E from the archaeon Methanobacterium thermoautotrophicum is a component of the 30S ribosomal subunit. S17E is a 62-residue protein conserved in archaea and eukaryotes and has no counterparts in bacteria...
  16. Shlimon A, Friedrich M, Niemann H, Ramsing N, Finster K. Methanobacterium aarhusense sp. nov., a novel methanogen isolated from a marine sediment (Aarhus Bay, Denmark). Int J Syst Evol Microbiol. 2004;54:759-63 pubmed
    ..rRNA gene comparison with sequences of known methanogens, strain H2-LR(T) could be affiliated to the genus Methanobacterium. The strain forms a distinct line of descent within this genus, with Methanobacterium oryzae (95...
  17. Guti rrez P, Osborne M, Siddiqui N, Trempe J, Arrowsmith C, Gehring K. Structure of the archaeal translation initiation factor aIF2 beta from Methanobacterium thermoautotrophicum: implications for translation initiation. Protein Sci. 2004;13:659-67 pubmed publisher
    ..We have determined the solution structure of the intact beta-subunit of aIF2 from Methanobacterium thermoautotrophicum...
  18. N lling J, Pihl T, Vriesema A, Reeve J. Organization and growth phase-dependent transcription of methane genes in two regions of the Methanobacterium thermoautotrophicum genome. J Bacteriol. 1995;177:2460-8 pubmed
    Two regions of the Methanobacterium thermoautotrophicum genome containing genes that encode enzymes involved in methanogenesis (methane genes) have been cloned and sequenced to determine the extent of methane gene clustering and ..
  19. N lling J, Ishii M, Koch J, Pihl T, Reeve J, Thauer R, et al. Characterization of a 45-kDa flavoprotein and evidence for a rubredoxin, two proteins that could participate in electron transport from H2 to CO2 in methanogenesis in Methanobacterium thermoautotrophicum. Eur J Biochem. 1995;231:628-38 pubmed
    b>Methanobacterium thermoautotrophicum strains contain a flavoprotein (flavoprotein A) that copurifies with the H2:heterodisulfide oxidoreductase complex...
  20. Li Y, Berke I, Chen L, Jiang Y. Gating and inward rectifying properties of the MthK K+ channel with and without the gating ring. J Gen Physiol. 2007;129:109-20 pubmed
    In MthK, a Ca2+-gated K+ channel from Methanobacterium thermoautotrophicum, eight cytoplasmic RCK domains form an octameric gating ring that controls the intracellular gate of the ion conduction pore...
  21. Liu Y, Zhang N, Liu X, Wang X, Wang Z, Chen Y, et al. Molecular mechanism underlying the interaction of typical Sac10b family proteins with DNA. PLoS ONE. 2012;7:e34986 pubmed publisher
    ..Here, we determined the crystal structure of Mth10b, an atypical member of the Sac10b family from Methanobacterium thermoautotrophicum ?H, at 2.2 Å...
  22. Kotsyurbenko O, Friedrich M, Simankova M, Nozhevnikova A, Golyshin P, Timmis K, et al. shift from acetoclastic to H2-dependent methanogenesis in a west Siberian peat bog at low pH values and isolation of an acidophilic Methanobacterium strain. Appl Environ Microbiol. 2007;73:2344-8 pubmed
    ..8, and Methanobacteriaceae became important except for Methanomicrobiaceae and Methanosarcinaceae. An acidophilic and psychrotolerant Methanobacterium sp. was isolated using H(2)-plus-CO(2)-supplemented medium at pH 4.5.
  23. Fletcher R, Chen X. Biochemical activities of the BOB1 mutant in Methanobacterium thermoautotrophicum MCM. Biochemistry. 2006;45:462-7 pubmed
    Minichromosomal maintenance proteins (MCMs) are considered to be the replicative helicase. Methanobacterium thermoautotrophicum has a single MCM gene (mtMCM). The crystal structure of the mtMCM N-terminal region is a double hexamer...
  24. Ma K, Liu X, Dong X. Methanobacterium beijingense sp. nov., a novel methanogen isolated from anaerobic digesters. Int J Syst Evol Microbiol. 2005;55:325-9 pubmed
    ..9 mol%. The similarity levels of the 16S rRNA sequence of strain 8-2T with other species of the genus Methanobacterium ranged from 93.8 to 96.0 %...
  25. N lling J, de Vos W. Characterization of the archaeal, plasmid-encoded type II restriction-modification system MthTI from Methanobacterium thermoformicicum THF: homology to the bacterial NgoPII system from Neisseria gonorrhoeae. J Bacteriol. 1992;174:5719-26 pubmed
    ..system, designated MthTI, was localized on plasmid pFV1 from the thermophilic archaeon Methanobacterium thermoformicicum THF...
  26. Moser D, Gihring T, Brockman F, Fredrickson J, Balkwill D, Dollhopf M, et al. Desulfotomaculum and Methanobacterium spp. dominate a 4- to 5-kilometer-deep fault. Appl Environ Microbiol. 2005;71:8773-83 pubmed
    ..and an archaeal species related to Methanobacterium spp...
  27. McFarlan S, Terrell C, Hogenkamp H. The purification, characterization, and primary structure of a small redox protein from Methanobacterium thermoautotrophicum, an archaebacterium. J Biol Chem. 1992;267:10561-9 pubmed
    ..has been purified to homogeneity from cell-free extracts of the strictly anaerobic thermophilic methanogen, Methanobacterium thermoautotrophicum (strain Marburg)...
  28. Setzke E, Hedderich R, Heiden S, Thauer R. H2: heterodisulfide oxidoreductase complex from Methanobacterium thermoautotrophicum. Composition and properties. Eur J Biochem. 1994;220:139-48 pubmed
    ..In this study, we show that in Methanobacterium thermoautotrophicum (strain Marburg) this reaction is catalyzed by a stable H2-heterodisulfide oxidoreductase ..
  29. Ciulla R, Clougherty C, Belay N, Krishnan S, Zhou C, Byrd D, et al. Halotolerance of Methanobacterium thermoautotrophicum delta H and Marburg. J Bacteriol. 1994;176:3177-87 pubmed
    b>Methanobacterium thermoautotrophicum delta H and Marburg were adapted to grow in medium containing up to 0.65 M NaCl. From 0.01 to 0.5 M NaCl, there was a lag before cell growth which increased with increasing external NaCl...
  30. Hochheimer A, Hedderich R, Thauer R. The formylmethanofuran dehydrogenase isoenzymes in Methanobacterium wolfei and Methanobacterium thermoautotrophicum: induction of the molybdenum isoenzyme by molybdate and constitutive synthesis of the tungsten isoenzyme. Arch Microbiol. 1998;170:389-93 pubmed
    ..b>Methanobacterium wolfei and Methanobacterium thermoautotrophicum have been shown to contain two isoenzymes, a tungsten-..
  31. Cort J, Yee A, Edwards A, Arrowsmith C, Kennedy M. Structure-based functional classification of hypothetical protein MTH538 from Methanobacterium thermoautotrophicum. J Mol Biol. 2000;302:189-203 pubmed publisher
    The structure of MTH538, a previously uncharacterized hypothetical protein from Methanobacterium thermoautotrophicum, has been determined by NMR spectroscopy...
  32. N lling J, de Vos W. Identification of the CTAG-recognizing restriction-modification systems MthZI and MthFI from Methanobacterium thermoformicicum and characterization of the plasmid-encoded mthZIM gene. Nucleic Acids Res. 1992;20:5047-52 pubmed
    ..and modification (R/M) systems, designated MthZI and MthFI, were identified in the thermophilic archaeon Methanobacterium thermoformicicum strains Z-245 and FTF, respectively...
  33. Zadek B, Nimigean C. Calcium-dependent gating of MthK, a prokaryotic potassium channel. J Gen Physiol. 2006;127:673-85 pubmed
    ..We were able to capture the mechanistic features of MthK with a modified MWC model...
  34. Hanzelka B, Darcy T, Reeve J. TFE, an archaeal transcription factor in Methanobacterium thermoautotrophicum related to eucaryal transcription factor TFIIEalpha. J Bacteriol. 2001;183:1813-8 pubmed publisher
    In the archaeon Methanobacterium thermoautotrophicum, MTH1669 encodes a protein with a sequence related to the N-terminal sequences of the alpha-subunits of eucaryal general transcription factor TFIIE...
  35. Gast D, Jenal U, Wasserfallen A, Leisinger T. Regulation of tryptophan biosynthesis in Methanobacterium thermoautotrophicum Marburg. J Bacteriol. 1994;176:4590-6 pubmed
    A tryptophan-auxotrophic mutant of the archaeon Methanobacterium thermoautotrophicum Marburg was grown with growth-promoting and growth-limiting concentrations of tryptophan...
  36. Kelman Z, Hurwitz J. A unique organization of the protein subunits of the DNA polymerase clamp loader in the archaeon Methanobacterium thermoautotrophicum deltaH. J Biol Chem. 2000;275:7327-36 pubmed
    ..Here we report the isolation and characterization of homologues of RFC and PCNA from the archaeon, Methanobacterium thermoautotrophicum DeltaH...
  37. Wu B, Yee A, Pineda Lucena A, Semesi A, Ramelot T, Cort J, et al. Solution structure of ribosomal protein S28E from Methanobacterium thermoautotrophicum. Protein Sci. 2003;12:2831-7 pubmed publisher
    The ribosomal protein S28E from the archaeon Methanobacterium thermoautotrophicum is a component of the 30S ribosomal subunit. Sequence homologs of S28E are found only in archaea and eukaryotes...
  38. Saridakis V, Shahinas D, Xu X, Christendat D. Structural insight on the mechanism of regulation of the MarR family of proteins: high-resolution crystal structure of a transcriptional repressor from Methanobacterium thermoautotrophicum. J Mol Biol. 2008;377:655-67 pubmed publisher
    ..Electrophoretic mobility shift assay and biophysical analyses further suggest that salicylate inactivates MTH313 and prevents it from binding to its promoter region...
  39. Sastry M, Robertson D, Moynihan J, Roberts M. Enzymatic degradation of cyclic 2,3-diphosphoglycerate to 2,3-diphosphoglycerate in Methanobacterium thermoautotrophicum. Biochemistry. 1992;31:2926-35 pubmed
    ..to be the product of the enzymatic degradation of cyclic 2,3-diphosphoglycerate (cDPG) in the archaebacterium Methanobacterium thermoautotrophicum delta H...
  40. Kotelnikova S, Macario A, Pedersen K. Methanobacterium subterraneum sp. nov., a new alkaliphilic, eurythermic and halotolerant methanogen isolated from deep granitic groundwater. Int J Syst Bacteriol. 1998;48 Pt 2:357-67 pubmed
    ..Phylogenetic studies based upon 16S rRNA gene sequence comparisons placed the isolate A8p in the genus Methanobacterium. Phenotypic and phylogenetic characters indicate that the alkaliphilic, halotolerant strain A8p represents a ..
  41. Chakrapani S, Perozo E. How to gate an ion channel: lessons from MthK. Nat Struct Mol Biol. 2007;14:180-2 pubmed
  42. N lling J, Reeve J. Growth- and substrate-dependent transcription of the formate dehydrogenase (fdhCAB) operon in Methanobacterium thermoformicicum Z-245. J Bacteriol. 1997;179:899-908 pubmed
    The formate dehydrogenase-encoding fdhCAB operon and flanking genes have been cloned and sequenced from Methanobacterium thermoformicicum Z-245...
  43. Nishihara M, Koga Y. Purification and properties of sn-glycerol-1-phosphate dehydrogenase from Methanobacterium thermoautotrophicum: characterization of the biosynthetic enzyme for the enantiomeric glycerophosphate backbone of ether polar lipids of Archaea. J Biochem. 1997;122:572-6 pubmed
    ..backbone (sn-glycerol-1-phosphate) of archaeal ether lipids was purified from a methanogenic archaeon, Methanobacterium thermoautotrophicum, and characterized...
  44. Sivaraman J, Myers R, Boju L, Sulea T, Cygler M, Jo Davisson V, et al. Crystal structure of Methanobacterium thermoautotrophicum phosphoribosyl-AMP cyclohydrolase HisI. Biochemistry. 2005;44:10071-80 pubmed publisher
    ..The three-dimensional structure of PR-AMP cyclohydrolase from Methanobacterium thermoautotrophicum was solved and refined to 1.7 A resolution. The enzyme is a homodimer...
  45. Hochheimer A, Hedderich R, Thauer R. The DNA binding protein Tfx from Methanobacterium thermoautotrophicum: structure, DNA binding properties and transcriptional regulation. Mol Microbiol. 1999;31:641-50 pubmed
    In Methanobacterium thermoautotrophicum, the fmdECB operon encoding the molybdenum formyl-methanofuran dehydrogenase is directly preceded by an open reading frame tfx predicted to encode a DNA binding protein. The 16...
  46. Saridakis V, Pai E. Mutational, structural, and kinetic studies of the ATP-binding site of Methanobacterium thermoautotrophicum nicotinamide mononucleotide adenylyltransferase. J Biol Chem. 2003;278:34356-63 pubmed publisher
    Several residues lining the ATP-binding site of Methanobacterium thermoautotrophicum nicotinamide mononucleotide adenylyltransferase (NMNATase) were mutated in an effort to better characterize their roles in substrate binding and ..
  47. Wasserfallen A, NOLLING J, Pfister P, Reeve J, Conway De Macario E. Phylogenetic analysis of 18 thermophilic Methanobacterium isolates supports the proposals to create a new genus, Methanothermobacter gen. nov., and to reclassify several isolates in three species, Methanothermobacter thermautotrophicus comb. nov., Me. Int J Syst Evol Microbiol. 2000;50 Pt 1:43-53 pubmed
    ..of new and published data, the phylogenetic position of 18 thermophilic isolates currently classified as Methanobacterium species was reinvestigated...
  48. Mackereth C, Arrowsmith C, Edwards A, McIntosh L. Zinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum. Proc Natl Acad Sci U S A. 2000;97:6316-21 pubmed
    ..Structure determination of this RNA polymerase subunit from Methanobacterium thermoautotrophicum reveals a topology, which we term a zinc-bundle, consisting of three alpha-helices ..
  49. Sousa D, Smidt H, Alves M, Stams A. Syntrophomonas zehnderi sp. nov., an anaerobe that degrades long-chain fatty acids in co-culture with Methanobacterium formicicum. Int J Syst Evol Microbiol. 2007;57:609-15 pubmed
    ..syntrophic fatty-acid-oxidizing bacterium, designated strain OL-4(T), was isolated as a co-culture with Methanobacterium formicicum DSM 1535(NT) from an anaerobic expanded granular sludge bed reactor used to treat an oleate-based ..
  50. Jiang Y, Lee A, Chen J, Cadene M, Chait B, MacKinnon R. The open pore conformation of potassium channels. Nature. 2002;417:523-6 pubmed publisher
    ..The open conformation favours high conduction by compressing the membrane field to the selectivity filter, and also permits large organic cations and inactivation peptides to enter the pore from the intracellular solution...
  51. Grabarse W, Mahlert F, Shima S, Thauer R, Ermler U. Comparison of three methyl-coenzyme M reductases from phylogenetically distant organisms: unusual amino acid modification, conservation and adaptation. J Mol Biol. 2000;303:329-44 pubmed publisher
    ..growth temperature optimum, 98 degrees C) were determined and compared with the known structure of MCR from Methanobacterium thermoautotrophicum (growth temperature optimum, 65 degrees C). The active sites of MCR from M. barkeri and M...
  52. Zhu J, Liu X, Dong X. Methanobacterium movens sp. nov. and Methanobacterium flexile sp. nov., isolated from lake sediment. Int J Syst Evol Microbiol. 2011;61:2974-8 pubmed publisher
    ..Phylogenetic analysis based on 16S rRNA gene sequences placed the two isolates in the genus Methanobacterium. Strain TS-2(T) was most closely related to Methanobacterium alcaliphilum NBRC 105226(T) (96% 16S rRNA gene ..
  53. Tersteegen A, Hedderich R. Methanobacterium thermoautotrophicum encodes two multisubunit membrane-bound [NiFe] hydrogenases. Transcription of the operons and sequence analysis of the deduced proteins. Eur J Biochem. 1999;264:930-43 pubmed
    ..each encoding a putative multisubunit membrane-bound [NiFe] hydrogenase, were identified in the genome of Methanobacterium thermoautotrophicum...
  54. Kuhner C, Lindenbach B, Wolfe R. Component A2 of methylcoenzyme M reductase system from Methanobacterium thermoautotrophicum delta H: nucleotide sequence and functional expression by Escherichia coli. J Bacteriol. 1993;175:3195-203 pubmed
    The gene for component A2 of the methylcoenzyme M reductase system from Methanobacterium thermoautotrophicum delta H was cloned, and its nucleotide sequence was determined...
  55. Ye S, Li Y, Jiang Y. Novel insights into K+ selectivity from high-resolution structures of an open K+ channel pore. Nat Struct Mol Biol. 2010;17:1019-23 pubmed publisher
    ..This single K+ binding at low concentration effectively blocks the permeation of Na+, providing a structural basis for the anomalous mole-fraction effect, a key property of multi-ion pores...
  56. Harms U, Weiss D, G rtner P, Linder D, Thauer R. The energy conserving N5-methyltetrahydromethanopterin:coenzyme M methyltransferase complex from Methanobacterium thermoautotrophicum is composed of eight different subunits. Eur J Biochem. 1995;228:640-8 pubmed
    N5-Methyltetrahydromethanopterin:coenzyme M methyltransferase (Mtr) from Methanobacterium thermoautotrophicum strain Marburg is a membrane-associated enzyme complex which catalyzes an energy-conserving, sodium-ion-translocating step in ..
  57. Dong J, Shi N, Berke I, Chen L, Jiang Y. Structures of the MthK RCK domain and the effect of Ca2+ on gating ring stability. J Biol Chem. 2005;280:41716-24 pubmed publisher
    MthK is a Ca2+-gated K+ channel from Methanobacterium autotrophicum. The crystal structure of the MthK channel in a Ca2+-bound open state was previously determined at 3...
  58. Gómez Llorente Y, Fletcher R, Chen X, Carazo J, San Martín C. Polymorphism and double hexamer structure in the archaeal minichromosome maintenance (MCM) helicase from Methanobacterium thermoautotrophicum. J Biol Chem. 2005;280:40909-15 pubmed
    b>Methanobacterium thermoautotrophicum minichromosome maintenance complex (mtMCM), a cellular replicative helicase, is a useful model for the more complex eukaryotic MCMs...
  59. Jiang Y, Lee A, Chen J, Cadene M, Chait B, MacKinnon R. Crystal structure and mechanism of a calcium-gated potassium channel. Nature. 2002;417:515-22 pubmed publisher
    ..expressed, analysed electrical properties, and determined the crystal structure of a K(+) channel (MthK) from Methanobacterium thermoautotrophicum in the Ca(2+)-bound, opened state...
  60. Pfister P, Wasserfallen A, Stettler R, Leisinger T. Molecular analysis of Methanobacterium phage psiM2. Mol Microbiol. 1998;30:233-44 pubmed
    The methanogenic archaeon Methanobacterium thermoautotrophicum Marburg is infected by the double-stranded DNA phage psiM2. The complete phage genome sequence of 26 111 bp was established...
  61. Vermeij P, van der Steen R, Keltjens J, Vogels G, Leisinger T. Coenzyme F390 synthetase from Methanobacterium thermoautotrophicum Marburg belongs to the superfamily of adenylate-forming enzymes. J Bacteriol. 1996;178:505-10 pubmed
    ..To gain more insight into the mechanism of the former reaction, coenzyme F390 synthetase from Methanobacterium thermoautotrophicum Marburg was purified 89-fold from cell extract to a specific activity of 0.75 mumol.min-1...
  62. G rtner P, Ecker A, Fischer R, Linder D, Fuchs G, Thauer R. Purification and properties of N5-methyltetrahydromethanopterin:coenzyme M methyltransferase from Methanobacterium thermoautotrophicum. Eur J Biochem. 1993;213:537-45 pubmed
    ..It catalyzes an energy-conserving step in methane formation from CO2 and from acetate. The enzyme from Methanobacterium thermoautotrophicum (strain Marburg) has been purified 30-fold to apparent homogeneity...
  63. Julien O, Gignac I, Hutton A, Yee A, Arrowsmith C, Gagné S. MTH187 from Methanobacterium thermoautotrophicum has three HEAT-like repeats. J Biomol NMR. 2006;35:149-54 pubmed
    ..is one of the 424 non-membrane proteins that were cloned and purified for the structural proteomic project of Methanobacterium thermoautotrophicum [Christendat, D., Yee, A., Dharamsi, A., Kluger, Y., Gerstein, M., Arrowsmith, C.H...
  64. Strop P, Smith K, Iverson T, Ferry J, Rees D. Crystal structure of the "cab"-type beta class carbonic anhydrase from the archaeon Methanobacterium thermoautotrophicum. J Biol Chem. 2001;276:10299-305 pubmed publisher
    The structure of the "cab"-type beta class carbonic anhydrase from the archaeon Methanobacterium thermoautotrophicum (Cab) has been determined to 2.1-A resolution using the multiwavelength anomalous diffraction phasing technique...
  65. Yee A, Booth V, Dharamsi A, Engel A, Edwards A, Arrowsmith C. Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum. Proc Natl Acad Sci U S A. 2000;97:6311-5 pubmed
    ..We have determined the solution structure of the RPB5 subunit from the thermophilic archeon, Methanobacterium thermoautotrophicum...
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    The complete 1,751,377-bp sequence of the genome of the thermophilic archaeon Methanobacterium thermoautotrophicum deltaH has been determined by a whole-genome shotgun sequencing approach...
  67. Stupperich E, Juza A, Hoppert M, Mayer F. Cloning, sequencing and immunological characterization of the corrinoid-containing subunit of the N5-methyltetrahydromethanopterin: coenzyme-M methyltransferase from Methanobacterium thermoautotrophicum. Eur J Biochem. 1993;217:115-21 pubmed
    A 3.5-kb EcoRI fragment of the Methanobacterium thermoautotrophicum chromosome contains five open reading frames, mtrA to mtrE...