methanobacteriaceae

Summary

Summary: A family of anaerobic, coccoid to rod-shaped METHANOBACTERIALES. Cell membranes are composed mainly of polyisoprenoid hydrocarbons ether-linked to glycerol. Its organisms are found in anaerobic habitats throughout nature.

Top Publications

  1. Wasserfallen A, NOLLING J, Pfister P, Reeve J, Conway De Macario E. Phylogenetic analysis of 18 thermophilic Methanobacterium isolates supports the proposals to create a new genus, Methanothermobacter gen. nov., and to reclassify several isolates in three species, Methanothermobacter thermautotrophicus comb. nov., Me. Int J Syst Evol Microbiol. 2000;50 Pt 1:43-53 pubmed
    ..Of the nine isolates formerly referred to as Methanobacterium thermoformicicum, six were reclassified as Methanothermobacter thermautotrophicus and three as Methanothermobacter wolfeii...
  2. Luo Y, Pfister P, Leisinger T, Wasserfallen A. Pseudomurein endoisopeptidases PeiW and PeiP, two moderately related members of a novel family of proteases produced in Methanothermobacter strains. FEMS Microbiol Lett. 2002;208:47-51 pubmed
    ..Database search results suggest that PeiW and PeiP are the first two experimentally identified members of a novel family of proteases in a superfamily of archaeal, bacterial, and eukaryotic protein homologs of animal transglutaminases. ..
  3. Farhoud M, Wessels H, Steenbakkers P, Mattijssen S, Wevers R, van Engelen B, et al. Protein complexes in the archaeon Methanothermobacter thermautotrophicus analyzed by blue native/SDS-PAGE and mass spectrometry. Mol Cell Proteomics. 2005;4:1653-63 pubmed
  4. Craft J, Horng Y, Ragsdale S, Brunold T. Spectroscopic and computational characterization of the nickel-containing F430 cofactor of methyl-coenzyme M reductase. J Biol Inorg Chem. 2004;9:77-89 pubmed
  5. Liu W, Pucci B, Rossi M, Pisani F, Ladenstein R. Structural analysis of the Sulfolobus solfataricus MCM protein N-terminal domain. Nucleic Acids Res. 2008;36:3235-43 pubmed publisher
    ..On this basis possible DNA unwinding mechanisms are discussed...
  6. Kato S, Kosaka T, Watanabe K. Comparative transcriptome analysis of responses of Methanothermobacter thermautotrophicus to different environmental stimuli. Environ Microbiol. 2008;10:893-905 pubmed
    ..These findings suggest that M. thermautotrophicus has complex transcriptional regulation mechanisms that facilitate it to survive in unstable ecosystems such as an anaerobic digester. ..
  7. Santangelo T, Reeve J. Archaeal RNA polymerase is sensitive to intrinsic termination directed by transcribed and remote sequences. J Mol Biol. 2006;355:196-210 pubmed
  8. Krishnan L, Sad S, Patel G, Sprott G. The potent adjuvant activity of archaeosomes correlates to the recruitment and activation of macrophages and dendritic cells in vivo. J Immunol. 2001;166:1885-93 pubmed
    ..Thus, the recruitment and activation of professional APCs by archaeosomes constitutes an efficient self-adjuvanting process for induction of Ag-specific responses to encapsulated Ags...
  9. Fricke W, Seedorf H, Henne A, Krüer M, Liesegang H, Hedderich R, et al. The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis. J Bacteriol. 2006;188:642-58 pubmed

More Information

Publications69

  1. Ishii S, Kosaka T, Hori K, Hotta Y, Watanabe K. Coaggregation facilitates interspecies hydrogen transfer between Pelotomaculum thermopropionicum and Methanothermobacter thermautotrophicus. Appl Environ Microbiol. 2005;71:7838-45 pubmed
  2. Nakamura K, Terada T, Sekiguchi Y, Shinzato N, Meng X, Enoki M, et al. Application of pseudomurein endoisopeptidase to fluorescence in situ hybridization of methanogens within the family Methanobacteriaceae. Appl Environ Microbiol. 2006;72:6907-13 pubmed
    In situ detection of methanogens within the family Methanobacteriaceae is sometimes known to be unsuccessful due to the difficulty in permeability of oligonucleotide probes...
  3. Dey M, Kunz R, Van Heuvelen K, Craft J, Horng Y, Tang Q, et al. Spectroscopic and computational studies of reduction of the metal versus the tetrapyrrole ring of coenzyme F430 from methyl-coenzyme M reductase. Biochemistry. 2006;45:11915-33 pubmed
    ..The spectroscopic and time-dependent DFT calculations add important insight into the electronic structure of the nickel hydrocorphinate in its Ni(II) and Ni(I) valence states...
  4. Shin J, Reeve J, Kelman Z. Use of a restriction enzyme-digested PCR product as substrate for helicase assays. Nucleic Acids Res. 2005;33:e8 pubmed
    ..Here, a PCR-based method to generate a substrate for a helicase assay is described, and its application for several archaeal, bacterial and viral enzymes is demonstrated...
  5. Steenbakkers P, Geerts W, Ayman Oz N, Keltjens J. Identification of pseudomurein cell wall binding domains. Mol Microbiol. 2006;62:1618-30 pubmed
    ..thermautotrophicus and of other pseudomurein-containing archaea...
  6. Hamann N, Mander G, Shokes J, Scott R, Bennati M, Hedderich R. A cysteine-rich CCG domain contains a novel [4Fe-4S] cluster binding motif as deduced from studies with subunit B of heterodisulfide reductase from Methanothermobacter marburgensis. Biochemistry. 2007;46:12875-85 pubmed
    ..In addition, Zn K-edge X-ray absorption spectroscopy identified an isolated Zn site with an S3(O/N)1 geometry in HdrB and the HDR holoenzyme. The N-terminal CCG domain is suggested to provide ligands to the Zn site...
  7. Luo H, Zhang H, Suzuki T, Hattori S, Kamagata Y. Differential expression of methanogenesis genes of Methanothermobacter thermoautotrophicus (formerly Methanobacterium thermoautotrophicum) in pure culture and in cocultures with fatty acid-oxidizing syntrophs. Appl Environ Microbiol. 2002;68:1173-9 pubmed
  8. Surín S, Cubonová L, Majerník A, McDermott P, Chong J, Smigán P. Isolation and characterization of an amiloride-resistant mutant of Methanothermobacter thermautotrophicus possessing a defective Na+/H+ antiport. FEMS Microbiol Lett. 2007;269:301-8 pubmed
    ..50% inhibition was observed in the mutant cells under the same conditions...
  9. Capaldi S, Berger J. Biochemical characterization of Cdc6/Orc1 binding to the replication origin of the euryarchaeon Methanothermobacter thermoautotrophicus. Nucleic Acids Res. 2004;32:4821-32 pubmed
    ..Our observations also indicate that the origin-binding properties of Cdc6/Orc1 proteins closely resemble those of bacterial DnaA...
  10. de Poorter L, Geerts W, Keltjens J. Hydrogen concentrations in methane-forming cells probed by the ratios of reduced and oxidized coenzyme F420. Microbiology. 2005;151:1697-705 pubmed
    ..The results of the study demonstrate that F420 is a useful probe to assess in situ hydrogen concentrations in H2-metabolizing methanogens...
  11. Seedorf H, Dreisbach A, Hedderich R, Shima S, Thauer R. F420H2 oxidase (FprA) from Methanobrevibacter arboriphilus, a coenzyme F420-dependent enzyme involved in O2 detoxification. Arch Microbiol. 2004;182:126-37 pubmed
    ..The presence of F(420)H(2) oxidase in methanogenic archaea may explain why some methanogens, e.g., the Methanobrevibacter species in the termite hindgut, cannot only tolerate but thrive under microoxic conditions...
  12. Godinic Mikulcic V, Jaric J, Hausmann C, Ibba M, Weygand Durasevic I. An archaeal tRNA-synthetase complex that enhances aminoacylation under extreme conditions. J Biol Chem. 2011;286:3396-404 pubmed publisher
    ..These data indicate that formation of a complex between MtSerRS and MtArgRS provides a means by which methanogenic archaea can optimize an early step in translation under a wide range of extreme environmental conditions...
  13. Kaster A, Moll J, Parey K, Thauer R. Coupling of ferredoxin and heterodisulfide reduction via electron bifurcation in hydrogenotrophic methanogenic archaea. Proc Natl Acad Sci U S A. 2011;108:2981-6 pubmed publisher
    ..This stoichiometry of coupling is consistent with an ATP gain per mole methane from 4 H(2) and CO(2) of near 0.5 deduced from an H(2)-threshold concentration of 8 Pa and a growth yield of up to 3 g/mol methane...
  14. Jenkinson E, Chong J. Minichromosome maintenance helicase activity is controlled by N- and C-terminal motifs and requires the ATPase domain helix-2 insert. Proc Natl Acad Sci U S A. 2006;103:7613-8 pubmed
    ..Deletion of the helix-2 insert additionally resulted in the abrogation of DNA unwinding. Our results provide significant insight into the molecular mechanisms used by the MCM helicase to both regulate and execute DNA unwinding...
  15. Kasiviswanathan R, Shin J, Kelman Z. DNA binding by the Methanothermobacter thermautotrophicus Cdc6 protein is inhibited by the minichromosome maintenance helicase. J Bacteriol. 2006;188:4577-80 pubmed publisher
    ..Using minichromosome maintenance (MCM) helicase mutant proteins unable to bind DNA, it was found that the interaction of MCM with Cdc6 inhibits the DNA binding activity of Cdc6...
  16. Oshikane H, Sheppard K, Fukai S, Nakamura Y, Ishitani R, Numata T, et al. Structural basis of RNA-dependent recruitment of glutamine to the genetic code. Science. 2006;312:1950-4 pubmed publisher
    ..tRNA(Gln) recognition by indirect readout based on shape complementarity of the D loop suggests an early anticodon-independent RNA-based mechanism for adding glutamine to the genetic code...
  17. Georg J, Schomacher L, Chong J, Majern k A, Raabe M, Urlaub H, et al. The Methanothermobacter thermautotrophicus ExoIII homologue Mth212 is a DNA uridine endonuclease. Nucleic Acids Res. 2006;34:5325-36 pubmed publisher
    ..The U-endo activity of Mth212 is served by the same catalytic center as its AP-endo activity...
  18. Shinzato N, Enoki M, Sato H, Nakamura K, Matsui T, Kamagata Y. Specific DNA binding of a potential transcriptional regulator, inosine 5'-monophosphate dehydrogenase-related protein VII, to the promoter region of a methyl coenzyme m reductase I-encoding operon retrieved from Methanothermobacter thermautotrophicus. Appl Environ Microbiol. 2008;74:6239-47 pubmed publisher
    ..The results presented here suggest that IMPDH VII encoded by MTH126 is a plausible candidate for the transcriptional regulator of the mcr operon in this methanogen...
  19. Costa A, Pape T, Van Heel M, Brick P, Patwardhan A, Onesti S. Structural basis of the Methanothermobacter thermautotrophicus MCM helicase activity. Nucleic Acids Res. 2006;34:5829-38 pubmed
    ..We propose a mechanistic model for the helicase activity, based on a ligand-controlled rotation of the AAA+ subunits...
  20. Ishii S, Kosaka T, Hotta Y, Watanabe K. Simulating the contribution of coaggregation to interspecies hydrogen fluxes in syntrophic methanogenic consortia. Appl Environ Microbiol. 2006;72:5093-6 pubmed
    ..We applied it to analyzing partially aggregated syntrophic cocultures with various substrates, revealing that large fractions of hydrogen molecules were fluxed in aggregates...
  21. Friedrich M. Methyl-coenzyme M reductase genes: unique functional markers for methanogenic and anaerobic methane-oxidizing Archaea. Methods Enzymol. 2005;397:428-42 pubmed
    ..It has also been suggested that anaerobic methane-oxidizing Archaea possess MCR, which facilitates detection of this novel group of "reverse methanogens" as well using the mcrA gene as a functional marker...
  22. Kasiviswanathan R, Shin J, Kelman Z. Interactions between the archaeal Cdc6 and MCM proteins modulate their biochemical properties. Nucleic Acids Res. 2005;33:4940-50 pubmed publisher
    ..The results also suggest that MCM and DNA may compete for Cdc6-1 protein binding. The implications of these observations for the initiation of archaeal DNA replication are discussed...
  23. Kasiviswanathan R, Shin J, Melamud E, Kelman Z. Biochemical characterization of the Methanothermobacter thermautotrophicus minichromosome maintenance (MCM) helicase N-terminal domains. J Biol Chem. 2004;279:28358-66 pubmed
    ..It is also shown that although oligomerization is not essential for single-stranded DNA binding and ATPase activity, the presence of domain C is essential for helicase activity...
  24. Karr E, Sandman K, Lurz R, Reeve J. TrpY regulation of trpB2 transcription in Methanothermobacter thermautotrophicus. J Bacteriol. 2008;190:2637-41 pubmed publisher
    ..Inhibitory complex formation is prevented by insertions within the regulatory region and by a G149R substitution in TrpY, even though TrpY(G149R) retains both TRP box DNA- and tryptophan-binding abilities...
  25. Hausmann C, Ibba M. Structural and functional mapping of the archaeal multi-aminoacyl-tRNA synthetase complex. FEBS Lett. 2008;582:2178-82 pubmed publisher
    ..Together with previous genetic data, these findings show that LeuRS plays a central role in mediating interactions within the archaeal MSC by acting as a core scaffolding protein...
  26. Mayr S, Latkoczy C, Krüger M, Gunther D, Shima S, Thauer R, et al. Structure of an F430 variant from archaea associated with anaerobic oxidation of methane. J Am Chem Soc. 2008;130:10758-67 pubmed publisher
    ..The more stable (17(2)R) isomer (80% at equilibrium) is an isolation artifact generated under the acidic conditions necessary for the isolation of the cofactors from the calcium carbonate-encrusted mats...
  27. Ingram Smith C, Smith K. AMP-forming acetyl-CoA synthetases in Archaea show unexpected diversity in substrate utilization. Archaea. 2007;2:95-107 pubmed
    ..Phylogenetic analysis, sequence alignment and structural modeling suggest a molecular basis for the altered substrate preference and expanded substrate range of AF-ACS2 versus MT-ACS1...
  28. Liesegang H, Kaster A, Wiezer A, Goenrich M, Wollherr A, Seedorf H, et al. Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism. J Bacteriol. 2010;192:5850-1 pubmed publisher
    ..The genomes of the two model methanogens differ substantially in protein coding sequences, in insertion sequence (IS)-like elements, and in clustered regularly interspaced short palindromic repeats (CRISPR) loci...
  29. Ding X, Yang W, Min H, Peng X, Zhou H, Lu Z. Isolation and characterization of a new strain of Methanothermobacter marburgensis DX01 from hot springs in China. Anaerobe. 2010;16:54-9 pubmed publisher
    ..marburgensis, namely M. marburgensis DX01. The isolation and characterization of the new M. marburgensis DX01 strain expands the known range of the Methanothermobacter genus...
  30. Dridi B, Fardeau M, Ollivier B, Raoult D, Drancourt M. The antimicrobial resistance pattern of cultured human methanogens reflects the unique phylogenetic position of archaea. J Antimicrob Chemother. 2011;66:2038-44 pubmed publisher
    ..Methanogenic archaea are constant members of the human oral and digestive microbiota retrieved, in particular, from periodontitis lesions. The objective of the study was to determine their susceptibility to antimicrobials...
  31. Guy C, Majerník A, Chong J, Bolt E. A novel nuclease-ATPase (Nar71) from archaea is part of a proposed thermophilic DNA repair system. Nucleic Acids Res. 2004;32:6176-86 pubmed
    ..A model of possible Nar71 function in DNA repair is presented...
  32. Vancek M, Vidová M, Majerník A, Smigán P. Methanogenesis is Ca2+ dependent in Methanothermobacter thermautotrophicus strain DeltaH. FEMS Microbiol Lett. 2006;258:269-73 pubmed
    ..Interestingly, our findings imply that one of the enzymes of methanogenesis might catalyse a Ca2+ -dependent step and allow a direct activation of methanogenesis by Ca2+ ions...
  33. Karr E. The methanogen-specific transcription factor MsvR regulates the fpaA-rlp-rub oxidative stress operon adjacent to msvR in Methanothermobacter thermautotrophicus. J Bacteriol. 2010;192:5914-22 pubmed publisher
    ..Oxidized and reduced preparations of MsvR demonstrated different EMSA binding patterns and regions of protection on the intergenic sequence, suggesting that MsvR may play a role in detecting the redox state of the cell...
  34. Dridi B, Raoult D, Drancourt M. Archaea as emerging organisms in complex human microbiomes. Anaerobe. 2011;17:56-63 pubmed publisher
    ..Future research aims to detect and culture additional human mucosa-associated Archaea and to look for their presence in additional human tissues, to establish their role in human infections involving complex flora...
  35. Pfeifer S, Greiner Stöffele T. A recombinant exonuclease III homologue of the thermophilic archaeon Methanothermobacter thermautotrophicus. DNA Repair (Amst). 2005;4:433-44 pubmed
    ..Considering the high intracellular potassium ion concentration in M. thermautotrophicus, our results suggest that the characterized thermophilic enzyme acts as an AP endonuclease in vivo with similar activities as Ape1...
  36. Luo Y, Pfister P, Leisinger T, Wasserfallen A. The genome of archaeal prophage PsiM100 encodes the lytic enzyme responsible for autolysis of Methanothermobacter wolfeii. J Bacteriol. 2001;183:5788-92 pubmed
    ..The ORF28 protein of PsiM100 was identified as the previously known autolytic enzyme pseudomurein endoisopeptidase PeiW produced by M. wolfeii...
  37. Thoma C, Frank M, Rachel R, Schmid S, Näther D, Wanner G, et al. The Mth60 fimbriae of Methanothermobacter thermoautotrophicus are functional adhesins. Environ Microbiol. 2008;10:2785-95 pubmed publisher
    ..In addition we proved that cells adhering to solid surfaces - organic and inorganic ones - express many more fimbriae than cells growing in liquid cultures. The Mth60 fimbriae therefore are used by M. thermoautotrophicus as adhesins...
  38. Greber B, Boehringer D, Godinic Mikulcic V, Crnkovic A, Ibba M, Weygand Durasevic I, et al. Cryo-EM structure of the archaeal 50S ribosomal subunit in complex with initiation factor 6 and implications for ribosome evolution. J Mol Biol. 2012;418:145-60 pubmed publisher
    ..The structure also provides a snapshot of the reductive evolution of the archaeal ribosome and offers new insights into the evolution of the translation system in archaea...
  39. Xie Y, Reeve J. Transcription by an archaeal RNA polymerase is slowed but not blocked by an archaeal nucleosome. J Bacteriol. 2004;186:3492-8 pubmed
    ..This inhibited transcription presumably by preventing archaeal TATA-box binding protein, general transcription factor TFB, and RNAP access and thus inhibiting transcription initiation...
  40. Kunz R, Dey M, Ragsdale S. Characterization of the thioether product formed from the thiolytic cleavage of the alkyl-nickel bond in methyl-coenzyme M reductase. Biochemistry. 2008;47:2661-7 pubmed publisher
  41. Sakakibara N, Kasiviswanathan R, Melamud E, Han M, Schwarz F, Kelman Z. Coupling of DNA binding and helicase activity is mediated by a conserved loop in the MCM protein. Nucleic Acids Res. 2008;36:1309-20 pubmed publisher
    ..Since similar residues are also conserved in the eukaryotic MCM proteins, the data presented here suggest a similar coupling between the N-terminal and catalytic domain of the eukaryotic enzyme...
  42. Ingram Smith C, Woods B, Smith K. Characterization of the acyl substrate binding pocket of acetyl-CoA synthetase. Biochemistry. 2006;45:11482-90 pubmed
    ..In particular, Trp416 appears to be the primary determinant for acyl chain length that can be accommodated in the binding site...
  43. Praetorius Ibba M, Rogers T, Samson R, Kelman Z, Ibba M. Association between Archaeal prolyl- and leucyl-tRNA synthetases enhances tRNA(Pro) aminoacylation. J Biol Chem. 2005;280:26099-104 pubmed publisher
    ..These findings indicate that ProRS and LeuRS associate in M. thermautotrophicus and suggest that this interaction contributes to translational fidelity by enhancing tRNA aminoacylation by ProRS...
  44. Costa A, van Duinen G, Medagli B, Chong J, Sakakibara N, Kelman Z, et al. Cryo-electron microscopy reveals a novel DNA-binding site on the MCM helicase. EMBO J. 2008;27:2250-8 pubmed publisher
    ..Our findings provide novel insights into the role of the MCM complex during the initiation step of DNA replication...
  45. Fletcher R, Shen J, Holden L, Chen X. Identification of amino acids important for the biochemical activity of Methanothermobacter thermautotrophicus MCM. Biochemistry. 2008;47:9981-6 pubmed publisher
  46. Shin J, Santangelo T, Xie Y, Reeve J, Kelman Z. Archaeal minichromosome maintenance (MCM) helicase can unwind DNA bound by archaeal histones and transcription factors. J Biol Chem. 2007;282:4908-15 pubmed
    ..In contrast, the minichromosome maintenance helicase was unable to unwind DNA bound by this archaeal RNA polymerase in a stalled transcript-elongating complex...
  47. Luo Y, Leisinger T, Wasserfallen A. Comparative sequence analysis of plasmids pME2001 and pME2200 of methanothermobacter marburgensis strains Marburg and ZH3. Plasmid. 2001;45:18-30 pubmed publisher
    ..marburgensis strain Marburg and may confer the apparent immunity of its host strain ZH3 to infection by phage psiM2. Our data indicate that M. marburgensis plasmids may evolve by a series of gene duplication and excision events...
  48. Dridi B, Henry M, Richet H, Raoult D, Drancourt M. Age-related prevalence of Methanomassiliicoccus luminyensis in the human gut microbiome. APMIS. 2012;120:773-7 pubmed publisher
    ..The prevalence of M. luminyensis significantly increased with age, contrary to M. smithii and M. stadtmanae...
  49. Praetorius Ibba M, Hausmann C, Paras M, Rogers T, Ibba M. Functional association between three archaeal aminoacyl-tRNA synthetases. J Biol Chem. 2007;282:3680-7 pubmed
    ..These findings, together with earlier data, indicate the existence of a functional complex of three aminoacyl-tRNA synthetases in archaea in which LeuRS improves the catalytic efficiency of tRNA aminoacylation by both LysRS and ProRS...
  50. Chen Y, Yu X, Kasiviswanathan R, Shin J, Kelman Z, Egelman E. Structural polymorphism of Methanothermobacter thermautotrophicus MCM. J Mol Biol. 2005;346:389-94 pubmed
    ..This offers new insight into the conformational dynamics of MCM and the phosphorylation-bypass phenotype in yeast...
  51. Ng C, Waterman D, Antson A, Ortiz Lombardía M. Structure of the Methanothermobacter thermautotrophicus exosome RNase PH ring. Acta Crystallogr D Biol Crystallogr. 2010;66:522-8 pubmed publisher
  52. Dridi B, Henry M, El Khéchine A, Raoult D, Drancourt M. High prevalence of Methanobrevibacter smithii and Methanosphaera stadtmanae detected in the human gut using an improved DNA detection protocol. PLoS ONE. 2009;4:e7063 pubmed publisher
    ..We developed a new protocol for the extraction and PCR-based detection of M. smithii and M. stadtmanae DNA in human stool...
  53. Krivushin K, Shcherbakova V, Petrovskaya L, Rivkina E. Methanobacterium veterum sp. nov., from ancient Siberian permafrost. Int J Syst Evol Microbiol. 2010;60:455-9 pubmed publisher
    ..nov., with the type strain MK4(T) (=DSM 19849(T) =VKM B-2440(T))...
  54. Pucci B, De Felice M, Rossi M, Onesti S, Pisani F. Amino acids of the Sulfolobus solfataricus mini-chromosome maintenance-like DNA helicase involved in DNA binding/remodeling. J Biol Chem. 2004;279:49222-8 pubmed
    ..These results have important implications for the understanding of the molecular mechanism of the MCM DNA helicase action...
  55. Namgoong S, Sheppard K, Sherrer R, Soll D. Co-evolution of the archaeal tRNA-dependent amidotransferase GatCAB with tRNA(Asn). FEBS Lett. 2007;581:309-14 pubmed
  56. Feng L, Sheppard K, Tumbula Hansen D, Soll D. Gln-tRNAGln formation from Glu-tRNAGln requires cooperation of an asparaginase and a Glu-tRNAGln kinase. J Biol Chem. 2005;280:8150-5 pubmed
    ..The activation of Glu-tRNA(Gln) via gamma-phosphorylation bears a similarity to the mechanism used by glutamine synthetase, which may point to an ancient link between glutamine synthesized for metabolism and translation...
  57. Shin J, Heo G, Kelman Z. The Methanothermobacter thermautotrophicus MCM helicase is active as a hexameric ring. J Biol Chem. 2009;284:540-6 pubmed publisher
    ..Using a combination of biochemical and structural analysis, it is shown here that the M. thermautotrophicus MCM helicase is active as a hexamer...
  58. Frank S, Deery E, Brindley A, Leech H, Lawrence A, Heathcote P, et al. Elucidation of substrate specificity in the cobalamin (vitamin B12) biosynthetic methyltransferases. Structure and function of the C20 methyltransferase (CbiL) from Methanothermobacter thermautotrophicus. J Biol Chem. 2007;282:23957-69 pubmed publisher
    ..The chemical logic underpinning the methylation is discussed...
  59. Hausmann C, Praetorius Ibba M, Ibba M. An aminoacyl-tRNA synthetase:elongation factor complex for substrate channeling in archaeal translation. Nucleic Acids Res. 2007;35:6094-102 pubmed
  60. Dey M, Kunz R, Lyons D, Ragsdale S. Characterization of alkyl-nickel adducts generated by reaction of methyl-coenzyme m reductase with brominated acids. Biochemistry. 2007;46:11969-78 pubmed
    ..The results demonstrate an unexpected reactivity and flexibility of the MCR active site in accommodating a broad range of substrates, which act as molecular rulers for the substrate channel in MCR...