haloferax volcanii

Summary

Summary: A species of halophilic archaea found in the Dead Sea.

Top Publications

  1. Rawls K, Martin J, Maupin Furlow J. Activity and transcriptional regulation of bacterial protein-like glycerol-3-phosphate dehydrogenase of the haloarchaea in Haloferax volcanii. J Bacteriol. 2011;193:4469-76 pubmed publisher
    ..Glycerol is catabolized solely by glycerol kinase (encoded by glpK) to glycerol-3-phosphate (G3P) in Haloferax volcanii. Here we characterized the next critical step of this metabolic pathway: the conversion of G3P to ..
  2. Brendel J, Stoll B, Lange S, Sharma K, Lenz C, Stachler A, et al. A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and stability of clustered regularly interspaced short palindromic repeats (crispr)-derived rnas (crrnas) in Haloferax volcanii. J Biol Chem. 2014;289:7164-77 pubmed publisher
    ..antiviral defense (Cascade)-like complex are needed for crRNA stability in the CRISPR-Cas type I-B system in Haloferax volcanii in vivo. Deletion of the cas6 gene results in the loss of mature crRNAs and interference...
  3. Tanaka M, Ogawa N, Ihara K, Sugiyama Y, Mukohata Y. Cytochrome aa(3) in Haloferax volcanii. J Bacteriol. 2002;184:840-5 pubmed
    A cytochrome in an extremely halophilic archaeon, Haloferax volcanii, was purified to homogeneity...
  4. Bitan Banin G, Ortenberg R, Mevarech M. Development of a gene knockout system for the halophilic archaeon Haloferax volcanii by use of the pyrE gene. J Bacteriol. 2003;185:772-8 pubmed
    So far, the extremely halophilic archaeon Haloferax volcanii has the best genetic tools among the archaea. However, the lack of an efficient gene knockout system for this organism has hampered further genetic studies...
  5. Fink Lavi E, Eichler J. Identification of residues essential for the catalytic activity of Sec11b, one of the two type I signal peptidases of Haloferax volcanii. FEMS Microbiol Lett. 2008;278:257-60 pubmed
    Sec11b is one of two signal peptidases (SPases) in the haloarchaeon Haloferax volcanii. Site-directed mutagenesis revealed Ser-72, His-137 and Asp-187 as essential for signal peptide cleavage...
  6. El Yacoubi B, Phillips G, Blaby I, Haas C, Cruz Y, Greenberg J, et al. A Gateway platform for functional genomics in Haloferax volcanii: deletion of three tRNA modification genes. Archaea. 2009;2:211-9 pubmed
    In part due to the existence of simple methods for its cultivation and genetic manipulation, Haloferax volcanii is a major archaeal model organism...
  7. Malki L, Yanku M, Borovok I, Cohen G, Mevarech M, Aharonowitz Y. Identification and characterization of gshA, a gene encoding the glutamate-cysteine ligase in the halophilic archaeon Haloferax volcanii. J Bacteriol. 2009;191:5196-204 pubmed publisher
    ..We report here the identification of the gshA gene in the extremely halophilic archaeon Haloferax volcanii and show that H. volcanii gshA directs in vivo the synthesis and accumulation of gamma GC...
  8. Irihimovitch V, Eichler J. Post-translational secretion of fusion proteins in the halophilic archaea Haloferax volcanii. J Biol Chem. 2003;278:12881-7 pubmed
    ..question, the signal peptide-encoding region of the surface layer glycoprotein gene from the Haloarchaea Haloferax volcanii was fused either to the cellulose-binding domain of the Clostridium thermocellum cellulosome or to the ..
  9. Hölzle A, Fischer S, Heyer R, Schütz S, Zacharias M, Walther P, et al. Maturation of the 5S rRNA 5' end is catalyzed in vitro by the endonuclease tRNase Z in the archaeon H. volcanii. RNA. 2008;14:928-37 pubmed publisher
    ..The 5S rRNA in the archaeon Haloferax volcanii is transcribed as part of a multicistronic transcript containing both large rRNAs and one or two tRNAs...

More Information

Publications118 found, 100 shown here

  1. Uthandi S, Saad B, Humbard M, Maupin Furlow J. LccA, an archaeal laccase secreted as a highly stable glycoprotein into the extracellular medium by Haloferax volcanii. Appl Environ Microbiol. 2010;76:733-43 pubmed publisher
    ..Here, we report the purification and characterization of a laccase (LccA) from the halophilic archaeon Haloferax volcanii. LccA was secreted at high levels into the culture supernatant of a recombinant H...
  2. Dambeck M, Soppa J. Characterization of a Haloferax volcanii member of the enolase superfamily: deletion mutant construction, expression analysis, and transcriptome comparison. Arch Microbiol. 2008;190:341-53 pubmed publisher
    ..function of an archaeal family member, an in frame deletion mutant of a gene encoding a COG4948 protein of Haloferax volcanii was generated...
  3. Abu Qarn M, Eichler J, Sharon N. Not just for Eukarya anymore: protein glycosylation in Bacteria and Archaea. Curr Opin Struct Biol. 2008;18:544-50 pubmed publisher
    ..Here, we discuss new structural and biosynthetic findings related to prokaryotic protein glycosylation, until recently a neglected topic. ..
  4. Gebetsberger J, Zywicki M, Künzi A, Polacek N. tRNA-derived fragments target the ribosome and function as regulatory non-coding RNA in Haloferax volcanii. Archaea. 2012;2012:260909 pubmed publisher
    ..Here we present evidence that tRFs from the halophilic archaeon Haloferax volcanii directly bind to ribosomes...
  5. Hering O, Brenneis M, Beer J, Suess B, Soppa J. A novel mechanism for translation initiation operates in haloarchaea. Mol Microbiol. 2009;71:1451-63 pubmed publisher
    ..Taken together, operation of a scanning mechanism was excluded and the results indicate that a novel mechanism for translation initiation operates at least in haloarchaea. ..
  6. Wanner C, Soppa J. Functional role for a 2-oxo acid dehydrogenase in the halophilic archaeon Haloferax volcanii. J Bacteriol. 2002;184:3114-21 pubmed
    The archaeon Haloferax volcanii was previously shown to contain and transcribe the genes for a 2-oxo acid dehydrogenase (OADH) complex, but their presence remained a mystery because no enzymatic activity with any of the known OADH ..
  7. Allers T, Ngo H, Mevarech M, Lloyd R. Development of additional selectable markers for the halophilic archaeon Haloferax volcanii based on the leuB and trpA genes. Appl Environ Microbiol. 2004;70:943-53 pubmed
    ..b>Haloferax volcanii offers great promise as a model organism for archaeal genetics, but until now there has been a lack of a ..
  8. Gil M, Sherwood K, Maupin Furlow J. Transcriptional linkage of Haloferax volcanii proteasomal genes with non-proteasomal gene neighbours including RNase P, MOSC domain and SAM-methyltransferase homologues. Microbiology. 2007;153:3009-22 pubmed
    ..into proteasome operon organization, transcripts of the five proteasomal genes of the halophilic archaeon Haloferax volcanii were analysed by Northern (RNA) blotting, RT-PCR and primer extension...
  9. Reuter C, Uthandi S, Puentes J, Maupin Furlow J. Hydrophobic carboxy-terminal residues dramatically reduce protein levels in the haloarchaeon Haloferax volcanii. Microbiology. 2010;156:248-55 pubmed publisher
    ..levels, using the reporter protein soluble modified red-shifted GFP (smRS-GFP) expressed in the haloarchaeon Haloferax volcanii as a model system...
  10. Eichler J, Maupin Furlow J. Post-translation modification in Archaea: lessons from Haloferax volcanii and other haloarchaea. FEMS Microbiol Rev. 2013;37:583-606 pubmed publisher
    ..Studies on halophilic archaea, in particular Haloferax volcanii, originally isolated from the Dead Sea, are helping to fill the void...
  11. Allers T, Barak S, Liddell S, Wardell K, Mevarech M. Improved strains and plasmid vectors for conditional overexpression of His-tagged proteins in Haloferax volcanii. Appl Environ Microbiol. 2010;76:1759-69 pubmed publisher
    ..b>Haloferax volcanii is widely regarded as the best-equipped organism for archaeal genetics, but the development of tools for the ..
  12. Thompson D, Daniels C. Heat shock inducibility of an archaeal TATA-like promoter is controlled by adjacent sequence elements. Mol Microbiol. 1998;27:541-51 pubmed
    ..chaperonin-containing Tcp-1) family member gene is regulated at the transcription level in the archaeon Haloferax volcanii. Transcriptional fusions of the cct1 promoter region with a yeast proline tRNA reporter gene were ..
  13. Delmas S, Duggin I, Allers T. DNA damage induces nucleoid compaction via the Mre11-Rad50 complex in the archaeon Haloferax volcanii. Mol Microbiol. 2013;87:168-79 pubmed publisher
    ..We show here that in the archaeon Haloferax volcanii, compaction and reorganization of the nucleoid is induced by stresses that damage the genome or interfere ..
  14. Lange C, Zerulla K, Breuert S, Soppa J. Gene conversion results in the equalization of genome copies in the polyploid haloarchaeon Haloferax volcanii. Mol Microbiol. 2011;80:666-77 pubmed publisher
    b>Haloferax volcanii is highly polyploid and contains about 20 copies of the major chromosome...
  15. Arbiv A, Yurist Doutsch S, Guan Z, Eichler J. AglQ is a novel component of the Haloferax volcanii N-glycosylation pathway. PLoS ONE. 2013;8:e81782 pubmed publisher
    ..Studies on the halophile Haloferax volcanii have offered insight into the archaeal version of this universal protein-processing event...
  16. Sisignano M, Morbitzer D, Gätgens J, Oldiges M, Soppa J. A 2-oxoacid dehydrogenase complex of Haloferax volcanii is essential for growth on isoleucine but not on other branched-chain amino acids. Microbiology. 2010;156:521-9 pubmed publisher
    The halophilic archaeon Haloferax volcanii contains three operons encoding 2-oxoacid dehydrogenase complexes (OADHCs) OADHC1-OADHC3...
  17. Hölzle A, Stoll B, Schnattinger T, Schöning U, Tjaden B, Marchfelder A. tRNA-like elements in Haloferax volcanii. Biochimie. 2012;94:940-6 pubmed publisher
    ..Here, we analysed 5S rRNA processing in the halophilic Archaeon Haloferax volcanii. Earlier experiments showed that the 5S rRNA is cleaved at its 5' end by the endonuclease tRNase Z...
  18. Humbard M, Stevens S, Maupin Furlow J. Posttranslational modification of the 20S proteasomal proteins of the archaeon Haloferax volcanii. J Bacteriol. 2006;188:7521-30 pubmed
    ..The halophilic archaeon Haloferax volcanii synthesizes two different alpha-type proteins, alpha1 and alpha2, and one beta-type protein that assemble ..
  19. Kirkland P, Reuter C, Maupin Furlow J. Effect of proteasome inhibitor clasto-lactacystin-beta-lactone on the proteome of the haloarchaeon Haloferax volcanii. Microbiology. 2007;153:2271-80 pubmed
    ..In this study, Haloferax volcanii was used as a model to determine the influence the proteasome-specific inhibitor clasto-lactacystin-beta-..
  20. Abu Qarn M, Yurist Doutsch S, Giordano A, Trauner A, Morris H, Hitchen P, et al. Haloferax volcanii AglB and AglD are involved in N-glycosylation of the S-layer glycoprotein and proper assembly of the surface layer. J Mol Biol. 2007;374:1224-36 pubmed
    In this study, the effects of deleting two genes previously implicated in Haloferax volcanii N-glycosylation on the assembly and attachment of a novel Asn-linked pentasaccharide decorating the H...
  21. Singh S, Gurha P, Gupta R. Dynamic guide-target interactions contribute to sequential 2'-O-methylation by a unique archaeal dual guide box C/D sRNP. RNA. 2008;14:1411-23 pubmed publisher
    ..Guide and target RNAs derived from Haloferax volcanii pre-tRNA(Trp) were used with recombinant Methanocaldococcus jannaschii core proteins in the reactions...
  22. Delmas S, Shunburne L, Ngo H, Allers T. Mre11-Rad50 promotes rapid repair of DNA damage in the polyploid archaeon Haloferax volcanii by restraining homologous recombination. PLoS Genet. 2009;5:e1000552 pubmed publisher
    ..We have studied DNA repair in the polyploid archaeon Haloferax volcanii, which contains up to 20 genome copies...
  23. Konrad Z, Eichler J. Protein glycosylation in Haloferax volcanii: partial characterization of a 98-kDa glycoprotein. FEMS Microbiol Lett. 2002;209:197-202 pubmed
    The plasma membrane of Haloferax volcanii contains several glycoproteins, including a 98-kDa species. Using lectin-based chromatography, the glycoprotein was isolated and partially characterized...
  24. Plösser P, Pfeifer F. A bZIP protein from halophilic archaea: structural features and dimer formation of cGvpE from Halobacterium salinarum. Mol Microbiol. 2002;45:511-20 pubmed
    ..protein, and mutations in the putative DNA binding domain DNAB completely abolish the activator function in Haloferax volcanii transformants...
  25. Large A, Stamme C, Lange C, Duan Z, Allers T, Soppa J, et al. Characterization of a tightly controlled promoter of the halophilic archaeon Haloferax volcanii and its use in the analysis of the essential cct1 gene. Mol Microbiol. 2007;66:1092-106 pubmed
    ..As tools for the genetic manipulation of Haloferax volcanii are well developed, we set out to identify promoters with a wide dynamic range of expression in this ..
  26. Gimenez M, Dilks K, Pohlschröder M. Haloferax volcanii twin-arginine translocation substates include secreted soluble, C-terminally anchored and lipoproteins. Mol Microbiol. 2007;66:1597-606 pubmed
    ..We have examined six computationally designated Tat substrates in the haloarchaeon Haloferax volcanii to verify previous computational predictions and to initiate studies of lipoprotein biogenesis via the Tat ..
  27. Sherwood K, Cano D, Maupin Furlow J. Glycerol-mediated repression of glucose metabolism and glycerol kinase as the sole route of glycerol catabolism in the haloarchaeon Haloferax volcanii. J Bacteriol. 2009;191:4307-15 pubmed publisher
    ..a gene encoding a glycerol kinase homolog (glpK; HVO_1541) was deleted from the genome of the haloarchaeon Haloferax volcanii by a markerless knockout strategy...
  28. Lestini R, Duan Z, Allers T. The archaeal Xpf/Mus81/FANCM homolog Hef and the Holliday junction resolvase Hjc define alternative pathways that are essential for cell viability in Haloferax volcanii. DNA Repair (Amst). 2010;9:994-1002 pubmed publisher
    ..We have studied the role of Hef in the euryarchaeon Haloferax volcanii. Unlike Xpf in eukaryotes, Hef is not involved in nucleotide excision repair; instead, this function is ..
  29. Kapatai G, Large A, Benesch J, Robinson C, Carrascosa J, Valpuesta J, et al. All three chaperonin genes in the archaeon Haloferax volcanii are individually dispensable. Mol Microbiol. 2006;61:1583-97 pubmed
    ..a detailed genetic, structural and biochemical analysis of these proteins in the halophilic archaeon, Haloferax volcanii. This organism contains three genes for group II chaperonins, and we show that all are individually ..
  30. Allers T, Ngo H. Genetic analysis of homologous recombination in Archaea: Haloferax volcanii as a model organism. Biochem Soc Trans. 2003;31:706-10 pubmed
    ..We are developing such systems using Haloferax volcanii as a model organism, as it can be cultivated in the laboratory with ease and offers great potential for ..
  31. Sp th B, Schubert S, Lieberoth A, Settele F, Sch tz S, Fischer S, et al. Two archaeal tRNase Z enzymes: similar but different. Arch Microbiol. 2008;190:301-8 pubmed publisher
    ..To investigate tRNA processing in archaea, we identified and expressed the tRNase Z from Haloferax volcanii, a halophilic archaeon. The recombinant enzyme is a homodimer and efficiently processes precursor tRNAs...
  32. Bidle K, Kirkland P, Nannen J, Maupin Furlow J. Proteomic analysis of Haloferax volcanii reveals salinity-mediated regulation of the stress response protein PspA. Microbiology. 2008;154:1436-43 pubmed publisher
    A proteomic survey of the halophilic archaeon Haloferax volcanii was performed by comparative two-dimensional gel electrophoresis in order to determine the molecular effects of salt stress on the organism...
  33. Soppa J, Straub J, Brenneis M, Jellen Ritter A, Heyer R, Fischer S, et al. Small RNAs of the halophilic archaeon Haloferax volcanii. Biochem Soc Trans. 2009;37:133-6 pubmed publisher
    ..experimental RNomics approaches were undertaken to search for the presence of sRNAs in the model archaeon Haloferax volcanii, resulting in more than 150 putative sRNA genes being identified...
  34. Tadeo X, López Méndez B, Trigueros T, Laín A, Castaño D, Millet O. Structural basis for the aminoacid composition of proteins from halophilic archea. PLoS Biol. 2009;7:e1000257 pubmed publisher
    ..Conversely, we quantitatively demonstrate that halophilicity is directly related to a decrease in the accessible surface area. ..
  35. Phillips G, Chikwana V, Maxwell A, El Yacoubi B, Swairjo M, Iwata Reuyl D, et al. Discovery and characterization of an amidinotransferase involved in the modification of archaeal tRNA. J Biol Chem. 2010;285:12706-13 pubmed publisher
    ..We constructed a Haloferax volcanii DeltatgtA2 derivative and demonstrated that tRNA from this strain lacks G(+) and instead accumulates preQ(0)...
  36. Kaminski L, Guan Z, Yurist Doutsch S, Eichler J. Two distinct N-glycosylation pathways process the Haloferax volcanii S-layer glycoprotein upon changes in environmental salinity. MBio. 2013;4:e00716-13 pubmed publisher
    ..In the haloarchaeon Haloferax volcanii, the surface (S)-layer glycoprotein can be simultaneously modified by two different N-glycans...
  37. Wright D, Banks D, Lohman J, Hilsenbeck J, Gloss L. The effect of salts on the activity and stability of Escherichia coli and Haloferax volcanii dihydrofolate reductases. J Mol Biol. 2002;323:327-44 pubmed
    ..The dihydrofolate reductases from Escherichia coli (ecDHFR) and two DHFR isozymes from Haloferax volcanii (hvDHFR1 and hvDHFR2) have been used as a model system to compare the effect of salts on a mesophilic and ..
  38. Lichi T, Ring G, Eichler J. Membrane binding of SRP pathway components in the halophilic archaea Haloferax volcanii. Eur J Biochem. 2004;271:1382-90 pubmed
    ..and SRP54, a central component of the signal recognition particle, was addressed in the halophilic archaea Haloferax volcanii. Purified H...
  39. Miranda H, Nembhard N, Su D, Hepowit N, Krause D, Pritz J, et al. E1- and ubiquitin-like proteins provide a direct link between protein conjugation and sulfur transfer in archaea. Proc Natl Acad Sci U S A. 2011;108:4417-22 pubmed publisher
    Based on our recent work with Haloferax volcanii, ubiquitin-like (Ubl) proteins (SAMP1 and SAMP2) are known to be covalently attached to proteins in archaea...
  40. Reuter C, Kaczowka S, Maupin Furlow J. Differential regulation of the PanA and PanB proteasome-activating nucleotidase and 20S proteasomal proteins of the haloarchaeon Haloferax volcanii. J Bacteriol. 2004;186:7763-72 pubmed
    The halophilic archaeon Haloferax volcanii produces three different proteins (alpha1, alpha2, and beta) that assemble into at least two 20S proteasome isoforms. This work reports the cloning and sequencing of two H...
  41. Al Mailem D, Hough D, Danson M. The 2-oxoacid dehydrogenase multienzyme complex of Haloferax volcanii. Extremophiles. 2008;12:89-96 pubmed
    ..In the current paper, we demonstrate that in Haloferax volcanii the four genes are transcribed as an operon in vivo...
  42. Altman Price N, Mevarech M. Genetic evidence for the importance of protein acetylation and protein deacetylation in the halophilic archaeon Haloferax volcanii. J Bacteriol. 2009;191:1610-7 pubmed publisher
    ..Sequence analysis of the genome of the haloarchaeon Haloferax volcanii led to the identification of three putative protein acetyltransferases belonging to the Gcn5 family, Pat1, ..
  43. Jeong Y, Jeong B, Song H. Crystal structure of ubiquitin-like small archaeal modifier protein 1 (SAMP1) from Haloferax volcanii. Biochem Biophys Res Commun. 2011;405:112-7 pubmed publisher
    ..the very recent characterization of ubiquitin-like small archaeal modifier proteins (SAMP1 and 2) from Haloferax volcanii. The ubiquitin (Ub) and Ubl molecules in eukaryotes have been studied extensively and their cellular ..
  44. Kirkland P, Humbard M, Daniels C, Maupin Furlow J. Shotgun proteomics of the haloarchaeon Haloferax volcanii. J Proteome Res. 2008;7:5033-9 pubmed publisher
    b>Haloferax volcanii, an extreme halophile originally isolated from the Dead Sea, is used worldwide as a model organism for furthering our understanding of archaeal cell physiology...
  45. Kaminski L, Abu Qarn M, Guan Z, Naparstek S, Ventura V, Raetz C, et al. AglJ adds the first sugar of the N-linked pentasaccharide decorating the Haloferax volcanii S-layer glycoprotein. J Bacteriol. 2010;192:5572-9 pubmed publisher
    Like the Eukarya and Bacteria, the Archaea also perform N glycosylation. Using the haloarchaeon Haloferax volcanii as a model system, a series of Agl proteins involved in the archaeal version of this posttranslational modification has ..
  46. Jantzer K, Zerulla K, Soppa J. Phenotyping in the archaea: optimization of growth parameters and analysis of mutants of Haloferax volcanii. FEMS Microbiol Lett. 2011;322:123-30 pubmed publisher
    A method to grow the halophilic archaeon Haloferax volcanii in microtiter plates has been optimized and now allows the parallel generation of very reproducible growth curves...
  47. Babski J, Tjaden B, Voss B, Jellen Ritter A, Marchfelder A, Hess W, et al. Bioinformatic prediction and experimental verification of sRNAs in the haloarchaeon Haloferax volcanii. RNA Biol. 2011;8:806-16 pubmed publisher
    ..led to the experimental identification of 21 intergenic and 18 antisense sRNA genes in the haloarchaeon Haloferax volcanii. To broaden the knowledge about sRNAs in haloarchaea, two bioinformatic approaches were used to predict sRNA ..
  48. Shi W, Tang X, Huang Y, Gan F, Tang B, Shen P. An extracellular halophilic protease SptA from a halophilic archaeon Natrinema sp. J7: gene cloning, expression and characterization. Extremophiles. 2006;10:599-606 pubmed
    ..The sptA gene was expressed in Haloferax volcanii WFD11, and the recombinant enzyme could be secreted into the medium as an active mature form...
  49. van Ooyen J, Soppa J. Three 2-oxoacid dehydrogenase operons in Haloferax volcanii: expression, deletion mutants and evolution. Microbiology. 2007;153:3303-13 pubmed
    ..Nevertheless, two gene clusters encoding OADHCs were found previously in Haloferax volcanii, but their biological function remained obscure. Here a third oadhc gene cluster of H. volcanii is presented...
  50. Zhou G, Kowalczyk D, Humbard M, Rohatgi S, Maupin Furlow J. Proteasomal components required for cell growth and stress responses in the haloarchaeon Haloferax volcanii. J Bacteriol. 2008;190:8096-105 pubmed publisher
    Little is known regarding the biological roles of archaeal proteases. The haloarchaeon Haloferax volcanii is an ideal model for understanding these enzymes, as it is one of few archaea with an established genetic system...
  51. Plavner N, Eichler J. Defining the topology of the N-glycosylation pathway in the halophilic archaeon Haloferax volcanii. J Bacteriol. 2008;190:8045-52 pubmed publisher
    ..with cysteine modification following site-directed mutagenesis, were enlisted to define the topology of Haloferax volcanii proteins experimentally proven to participate in the N-glycosylation process...
  52. Bidle K, Haramaty L, Baggett N, Nannen J, Bidle K. Tantalizing evidence for caspase-like protein expression and activity in the cellular stress response of Archaea. Environ Microbiol. 2010;12:1161-72 pubmed publisher
    ..Using the model haloarchaeon Haloferax volcanii, we document extremely high caspase-specific activity and expression of immunoreactive proteins to human ..
  53. Blaby I, Majumder M, Chatterjee K, Jana S, Grosjean H, De Crecy Lagard V, et al. Pseudouridine formation in archaeal RNAs: The case of Haloferax volcanii. RNA. 2011;17:1367-80 pubmed publisher
    ..Cbf5 is the core ? synthase in these complexes. Using Haloferax volcanii as an archaeal model organism, we show that, contrary to eukaryotes, the Cbf5 homolog (HVO_2493) is not ..
  54. Pickl A, Johnsen U, Sch nheit P. Fructose degradation in the haloarchaeon Haloferax volcanii involves a bacterial type phosphoenolpyruvate-dependent phosphotransferase system, fructose-1-phosphate kinase, and class II fructose-1,6-bisphosphate aldolase. J Bacteriol. 2012;194:3088-97 pubmed publisher
    The halophilic archaeon Haloferax volcanii utilizes fructose as a sole carbon and energy source...
  55. Heyer R, Dörr M, Jellen Ritter A, Späth B, Babski J, Jaschinski K, et al. High throughput sequencing reveals a plethora of small RNAs including tRNA derived fragments in Haloferax volcanii. RNA Biol. 2012;9:1011-8 pubmed publisher
    To define the complete sRNA population of the halophilic archaeon Haloferax volcanii, we employed high throughput sequencing...
  56. Wendoloski D, Ferrer C, Dyall Smith M. A new simvastatin (mevinolin)-resistance marker from Haloarcula hispanica and a new Haloferax volcanii strain cured of plasmid pHV2. Microbiology. 2001;147:959-64 pubmed publisher
    ..Plasmids bearing this marker suffer from instability in Haloferax volcanii because the resistance gene was derived from the genome of this species and is almost identical in sequence ..
  57. Bab Dinitz E, Shmuely H, Maupin Furlow J, Eichler J, Shaanan B. Haloferax volcanii PitA: an example of functional interaction between the Pfam chlorite dismutase and antibiotic biosynthesis monooxygenase families?. Bioinformatics. 2006;22:671-5 pubmed
    ..within a single open reading frame has been revealed by both sequence homology and structural modeling in Haloferax volcanii PitA and its homologues in other halophilic archaea...
  58. Ring G, Londei P, Eichler J. Protein biogenesis in Archaea: addressing translation initiation using an in vitro protein synthesis system for Haloferax volcanii. FEMS Microbiol Lett. 2007;270:34-41 pubmed
    ..To better understand translation initiation in Archaea, an in vitro translation system from the haloarchaeon Haloferax volcanii has been developed...
  59. Hammelmann M, Soppa J. Optimized generation of vectors for the construction of Haloferax volcanii deletion mutants. J Microbiol Methods. 2008;75:201-4 pubmed publisher
    A method for the construction of in frame deletions in chromosomal genes of the archaeon Haloferax volcanii has been described recently (Allers et al. (2004), Appl. Environ. Microbiol. 70:943-953)...
  60. Zhao A, Gray F, MacNeill S. ATP- and NAD+-dependent DNA ligases share an essential function in the halophilic archaeon Haloferax volcanii. Mol Microbiol. 2006;59:743-52 pubmed
    ..Using the genetically tractable halophile Haloferax volcanii as a model system, we describe the first genetic analysis of archaeal DNA ligase function...
  61. Brenneis M, Hering O, Lange C, Soppa J. Experimental characterization of Cis-acting elements important for translation and transcription in halophilic archaea. PLoS Genet. 2007;3:e229 pubmed
    ..The 5'-ends and 3'-ends of 40 transcripts of two haloarchaeal species, Halobacterium salinarum and Haloferax volcanii, have been determined...
  62. Yurist Doutsch S, Abu Qarn M, Battaglia F, Morris H, Hitchen P, Dell A, et al. AglF, aglG and aglI, novel members of a gene island involved in the N-glycosylation of the Haloferax volcanii S-layer glycoprotein. Mol Microbiol. 2008;69:1234-45 pubmed publisher
    ..step in ongoing efforts to identify components of the N-glycosylation pathway of the halophilic archaeon Haloferax volcanii, the involvement of three additional gene products in the biosynthesis of the pentasaccharide decorating the ..
  63. Naparstek S, Vinagradov E, Eichler J. Different glycosyltransferases are involved in lipid glycosylation and protein N-glycosylation in the halophilic archaeon Haloferax volcanii. Arch Microbiol. 2010;192:581-4 pubmed publisher
    ..involved in N-glycosylation of the surface-layer glycoprotein of the halophilic archaeon Haloferax volcanii. In the present study, the involvement of these same glycosyltransferases in the biosynthesis of Hfx...
  64. Naor A, Lazary R, Barzel A, Papke R, Gophna U. In vivo characterization of the homing endonuclease within the polB gene in the halophilic archaeon Haloferax volcanii. PLoS ONE. 2011;6:e15833 pubmed publisher
    ..In the halophilic archaeon, Haloferax volcanii, the gene encoding DNA polymerase B (polB) contains an intein with an annotated but uncharacterized HEN...
  65. Kaczowka S, Reuter C, Talarico L, Maupin Furlow J. Recombinant production of Zymomonas mobilis pyruvate decarboxylase in the haloarchaeon Haloferax volcanii. Archaea. 2005;1:327-34 pubmed
    ..or other useful products, the pyruvate decarboxylase gene of Zymomonas mobilis (Zm pdc) was expressed in Haloferax volcanii. This gene has been used successfully to channel pyruvate to ethanol in various Gram-negative bacteria, ..
  66. Abu Qarn M, Eichler J. Protein N-glycosylation in Archaea: defining Haloferax volcanii genes involved in S-layer glycoprotein glycosylation. Mol Microbiol. 2006;61:511-25 pubmed
    In this study, characterization of the N-glycosylation process in the haloarchaea Haloferax volcanii was undertaken. Initially, putative Hfx...
  67. Straub J, Brenneis M, Jellen Ritter A, Heyer R, Soppa J, Marchfelder A. Small RNAs in haloarchaea: identification, differential expression and biological function. RNA Biol. 2009;6:281-92 pubmed
    ..of small noncoding RNAs (sRNAs) in archaea we applied RNomics to identify sRNAs in the halophilic archaeon Haloferax volcanii. Using a size-selected cDNA library, 39 different previously uncharacterized sRNAs were identified ranging ..
  68. Kirkland P, Busby J, Stevens S, Maupin Furlow J. Trizol-based method for sample preparation and isoelectric focusing of halophilic proteins. Anal Biochem. 2006;351:254-9 pubmed
    ..Here, we describe a method of preparing protein samples from the haloarchaeon Haloferax volcanii that not only desalts the samples thoroughly but also drastically reduces the amount of protein loss ..
  69. Poidevin L, MacNeill S. Biochemical characterisation of LigN, an NAD+-dependent DNA ligase from the halophilic euryarchaeon Haloferax volcanii that displays maximal in vitro activity at high salt concentrations. BMC Mol Biol. 2006;7:44 pubmed publisher
    ..Among the archaeal NAD+-dependent DNA ligases is the LigN enzyme of the halophilic euryarchaeon Haloferax volcanii, the gene for which was apparently acquired by Hfx...
  70. Naor A, Lapierre P, Mevarech M, Papke R, Gophna U. Low species barriers in halophilic archaea and the formation of recombinant hybrids. Curr Biol. 2012;22:1444-8 pubmed publisher
    ..We show high recombination frequencies between Haloferax volcanii and Haloferax mediterranei, two species that have an average nucleotide sequence identity of 86.6%...
  71. Rose R, Pohlschr der M. In vivo analysis of an essential archaeal signal recognition particle in its native host. J Bacteriol. 2002;184:3260-7 pubmed
    ..RNA homologs (hv54h, hv19h, and hv7Sh, respectively) of the genetically and biochemically tractable archaeon Haloferax volcanii were cloned, providing the tools to analyze the SRP in its native host...
  72. Bortolin M, Bachellerie J, Clouet d Orval B. In vitro RNP assembly and methylation guide activity of an unusual box C/D RNA, cis-acting archaeal pre-tRNA(Trp). Nucleic Acids Res. 2003;31:6524-35 pubmed
  73. Guy C, Haldenby S, Brindley A, Walsh D, Briggs G, Warren M, et al. Interactions of RadB, a DNA repair protein in archaea, with DNA and ATP. J Mol Biol. 2006;358:46-56 pubmed
    ..between RadB and ATP using genetics and biochemistry; ATP binding by RadB is needed to promote survival of Haloferax volcanii after UV irradiation, and ATP, but not other NTPs, induces pronounced conformational change in RadB...
  74. Mackwan R, Carver G, Drake J, Grogan D. An unusual pattern of spontaneous mutations recovered in the halophilic archaeon Haloferax volcanii. Genetics. 2007;176:697-702 pubmed
    Spontaneous mutations in the orotate:phosphoribosyl transferase (pyrE2) gene of the halophilic archaeon Haloferax volcanii were selected by 5-fluoroorotic acid plus uracil at a rate of approximately 2 x 10(-8)/cell division in fluctuation ..
  75. Hechler T, Pfeifer F. Anaerobiosis inhibits gas vesicle formation in halophilic Archaea. Mol Microbiol. 2009;71:132-45 pubmed publisher
    ..vesicle formation was investigated in three Halobacterium salinarum strains, Haloferax mediterranei and in Haloferax volcanii transformants...
  76. Guan Z, Naparstek S, Calo D, Eichler J. Protein glycosylation as an adaptive response in Archaea: growth at different salt concentrations leads to alterations in Haloferax volcanii S-layer glycoprotein N-glycosylation. Environ Microbiol. 2012;14:743-53 pubmed publisher
    ..To test this hypothesis, N-glycosylation of the Haloferax volcanii S-layer glycoprotein was compared in cells grown in high (3.4 M NaCl) and low (1...
  77. Sprott G, Dicaire C, Gurnani K, Deschatelets L, Krishnan L. Liposome adjuvants prepared from the total polar lipids of Haloferax volcanii, Planococcus spp. and Bacillus firmus differ in ability to elicit and sustain immune responses. Vaccine. 2004;22:2154-62 pubmed
    ..stimulating activity was compared for lipid vesicles consisting of the total polar lipids of an archaeon Haloferax volcanii, and the eubacteria Planococcus spp. and Bacillus firmus...
  78. Reuter C, Maupin Furlow J. Analysis of proteasome-dependent proteolysis in Haloferax volcanii cells, using short-lived green fluorescent proteins. Appl Environ Microbiol. 2004;70:7530-8 pubmed
    ..by functional expression of a green fluorescent protein variant with C-terminal fusions in the haloarchaeon Haloferax volcanii. The levels of this reporter protein correlated with whole-cell fluorescence that was readily detected in ..
  79. Karadzic I, Maupin Furlow J. Improvement of two-dimensional gel electrophoresis proteome maps of the haloarchaeon Haloferax volcanii. Proteomics. 2005;5:354-9 pubmed
    ..9-5.1). This combination enabled the construction of highly reproducible 2-D maps of Haloferax volcanii proteins.
  80. Brenneis M, Soppa J. Regulation of translation in haloarchaea: 5'- and 3'-UTRs are essential and have to functionally interact in vivo. PLoS ONE. 2009;4:e4484 pubmed publisher
    ..differential growth phase-dependent translational control in the two species Halobacterium salinarum and Haloferax volcanii were as high as in eukaryotes...
  81. Han J, Lu Q, Zhou L, Liu H, Xiang H. Identification of the polyhydroxyalkanoate (PHA)-specific acetoacetyl coenzyme A reductase among multiple FabG paralogs in Haloarcula hispanica and reconstruction of the PHA biosynthetic pathway in Haloferax volcanii. Appl Environ Microbiol. 2009;75:6168-75 pubmed publisher
    ..genes (phaEC) together with fabG1, but not its five paralogs, reconstructed the PHA biosynthetic pathway in Haloferax volcanii, a PHA-defective haloarchaeon. Taken together, our results indicate that FabG1 in H...
  82. Tripepi M, Imam S, Pohlschr der M. Haloferax volcanii flagella are required for motility but are not involved in PibD-dependent surface adhesion. J Bacteriol. 2010;192:3093-102 pubmed publisher
    Although the genome of Haloferax volcanii contains genes (flgA1-flgA2) that encode flagellins and others that encode proteins involved in flagellar assembly, previous reports have concluded that H. volcanii is nonmotile...
  83. Bracken C, Neighbor A, Lamlenn K, Thomas G, Schubert H, Whitby F, et al. Crystal structures of a halophilic archaeal malate synthase from Haloferax volcanii and comparisons with isoforms A and G. BMC Struct Biol. 2011;11:23 pubmed publisher
    ..We have solved the structure of a malate synthase H (MSH) isoform member from Haloferax volcanii in complex with glyoxylate at 2...
  84. Trachtenberg S, Pinnick B, Kessel M. The cell surface glycoprotein layer of the extreme halophile Halobacterium salinarum and its relation to Haloferax volcanii: cryo-electron tomography of freeze-substituted cells and projection studies of negatively stained envelopes. J Struct Biol. 2000;130:10-26 pubmed
    ..envelopes we have calculated a two-dimensional, projection map that is strikingly similar to that of Haloferax volcanii, an extreme halophile requiring high concentrations of magnesium; both projection maps show the hexagonal ..
  85. Singh S, Gurha P, Tran E, Maxwell E, Gupta R. Sequential 2'-O-methylation of archaeal pre-tRNATrp nucleotides is guided by the intron-encoded but trans-acting box C/D ribonucleoprotein of pre-tRNA. J Biol Chem. 2004;279:47661-71 pubmed
    b>Haloferax volcanii pre-tRNA(Trp) processing requires box C/D ribonucleoprotein (RNP)-guided 2'-O-methylation of nucleotides C34 and U39 followed by intron excision...
  86. Humbard M, Miranda H, Lim J, Krause D, Pritz J, Zhou G, et al. Ubiquitin-like small archaeal modifier proteins (SAMPs) in Haloferax volcanii. Nature. 2010;463:54-60 pubmed publisher
    ..fold and carboxy-terminal diglycine motif similar to ubiquitin, that form protein conjugates in the archaeon Haloferax volcanii. The levels of SAMP-conjugates were altered by nitrogen-limitation and proteasomal gene knockout and spanned ..
  87. Blaby I, Phillips G, Blaby Haas C, Gulig K, El Yacoubi B, De Crecy Lagard V. Towards a systems approach in the genetic analysis of archaea: Accelerating mutant construction and phenotypic analysis in Haloferax volcanii. Archaea. 2010;2010:426239 pubmed publisher
    With the availability of a genome sequence and increasingly sophisticated genetic tools, Haloferax volcanii is becoming a model for both Archaea and halophiles. In order for H...
  88. Darling A, Tritt A, Eisen J, Facciotti M. Mauve assembly metrics. Bioinformatics. 2011;27:2756-7 pubmed publisher
  89. Cohen Rosenzweig C, Yurist Doutsch S, Eichler J. AglS, a novel component of the Haloferax volcanii N-glycosylation pathway, is a dolichol phosphate-mannose mannosyltransferase. J Bacteriol. 2012;194:6909-16 pubmed publisher
    In Haloferax volcanii, a series of Agl proteins mediates protein N-glycosylation. The genes encoding all but one of the Agl proteins are sequestered into a single gene island...
  90. Maier L, Lange S, Stoll B, Haas K, Fischer S, Fischer E, et al. Essential requirements for the detection and degradation of invaders by the Haloferax volcanii CRISPR/Cas system I-B. RNA Biol. 2013;10:865-74 pubmed publisher
    ..We analyzed the requirements for a successful defense reaction in the halophilic archaeon Haloferax volcanii. Haloferax encodes a CRISPR/Cas system of the I-B subtype, about which very little is known...
  91. Abu Qarn M, Giordano A, Battaglia F, Trauner A, Hitchen P, Morris H, et al. Identification of AglE, a second glycosyltransferase involved in N glycosylation of the Haloferax volcanii S-layer glycoprotein. J Bacteriol. 2008;190:3140-6 pubmed publisher
    ..the present report continues ongoing efforts to identify components involved in the N glycosylation of the Haloferax volcanii S-layer glycoprotein...
  92. Fischer S, John von Freyend S, Sabag Daigle A, Daniels C, Allers T, Marchfelder A. Assigning a function to a conserved archaeal metallo-?-lactamase from Haloferax volcanii. Extremophiles. 2012;16:333-43 pubmed publisher
    ..The protein HVO_2763 from Haloferax volcanii is such a potential metallo-?-lactamase...