haloferax

Summary

Summary: A genus of extremely halophilic HALOBACTERIACEAE which are chemoheterotropic and strictly aerobic. They are found in neutral saline environments such as salt lakes (especially the Dead Sea) and marine salterns.

Top Publications

  1. Patenge N, Haase A, Bolhuis H, Oesterhelt D. The gene for a halophilic beta-galactosidase (bgaH) of Haloferax alicantei as a reporter gene for promoter analyses in Halobacterium salinarum. Mol Microbiol. 2000;36:105-13 pubmed
    ..Recently, the gene for an extremely halophilic beta-galactosidase (bgaH) from Haloferax alicantei has become available...
  2. Portnoy V, Evguenieva Hackenberg E, Klein F, Walter P, Lorentzen E, Klug G, et al. RNA polyadenylation in Archaea: not observed in Haloferax while the exosome polynucleotidylates RNA in Sulfolobus. EMBO Rep. 2005;6:1188-93 pubmed
    ..In this work, we analysed RNA polyadenylation and degradation in the archaea Sulfolobus solfataricus and Haloferax volcanii. No RNA polyadenylation was detected in the halophilic archaeon H. volcanii...
  3. Elshahed M, Savage K, Oren A, Gutierrez M, Ventosa A, Krumholz L. Haloferax sulfurifontis sp. nov., a halophilic archaeon isolated from a sulfide- and sulfur-rich spring. Int J Syst Evol Microbiol. 2004;54:2275-9 pubmed
    ..16S rRNA gene sequence analysis indicated that strain M6(T) belongs to the family Halobacteriaceae, genus Haloferax; it showed 96.7-98...
  4. Bowers K, Wiegel J. Temperature and pH optima of extremely halophilic archaea: a mini-review. Extremophiles. 2011;15:119-28 pubmed publisher
    ..extreme halophiles which have elevated pH optima, only four taxa have an optimum temperature above 50°C: Haloarcula quadrata (52°C), Haloferax elongans (53°C), Haloferax mediterranei (51°C) and Natronolimnobius 'aegyptiacus' (55°C).
  5. Kuo Y, Thompson D, St Jean A, Charlebois R, Daniels C. Characterization of two heat shock genes from Haloferax volcanii: a model system for transcription regulation in the Archaea. J Bacteriol. 1997;179:6318-24 pubmed
    The expression of two heat-responsive cct (chaperonin-containing Tcp-1) genes from the archaeon Haloferax volcanii was investigated at the transcription level...
  6. Zafrilla B, Mart nez Espinosa R, Esclapez J, P rez Pomares F, Bonete M. SufS protein from Haloferax volcanii involved in Fe-S cluster assembly in haloarchaea. Biochim Biophys Acta. 2010;1804:1476-82 pubmed publisher
    ..In this study, we have cloned, expressed and characterised a cysteine desulphurase (SufS) from Haloferax volcanii and demonstrated that this protein is able to reconstitute the [Fe-S] cluster of halophilic ferredoxin.
  7. Paramonov N, Parolis L, Parolis H, Boán I, Anton J, Rodriguez Valera F. The structure of the exocellular polysaccharide produced by the Archaeon Haloferax gibbonsii (ATCC 33959). Carbohydr Res. 1998;309:89-94 pubmed
    The structure of the neutral exocellular polysaccharide isolated from the Archaeon Haloferax gibbonsii (ATCC 33959) has been determined using acid hydrolysis, methylation analysis and NMR spectroscopy...
  8. Parolis L, Parolis H, Paramonov N, Boán I, Anton J, Rodriguez Valera F. Structural studies on the acidic exopolysaccharide from Haloferax denitrificans ATCC 35960. Carbohydr Res. 1999;319:133-40 pubmed
    The structure of a linear, acidic exopolysaccharide isolated from the Archaeon Haloferax denitrificans ATCC 35960 has been determined using NMR spectroscopy...
  9. Burns D, Camakaris H, Janssen P, Dyall Smith M. Combined use of cultivation-dependent and cultivation-independent methods indicates that members of most haloarchaeal groups in an Australian crystallizer pond are cultivable. Appl Environ Microbiol. 2004;70:5258-65 pubmed
    ..examined, all belonged to the family Halobacteriaceae, including members related to species of the genera Haloferax, Halorubrum, and Natronomonas...

More Information

Publications89

  1. Poplawski A, Gullbrand B, Bernander R. The ftsZ gene of Haloferax mediterranei: sequence, conserved gene order, and visualization of the FtsZ ring. Gene. 2000;242:357-67 pubmed
    We sequenced the ftsZ gene region of the halophilic archaeon Haloferax mediterranei and mapped the transcription start sites for the ftsZ gene...
  2. Sprott G, Larocque S, Cadotte N, Dicaire C, McGee M, Brisson J. Novel polar lipids of halophilic eubacterium Planococcus H8 and archaeon Haloferax volcanii. Biochim Biophys Acta. 2003;1633:179-88 pubmed
    ..adjuvant activity, a structural comparison was made between the polar lipids from two halophiles, an archaeon Haloferax volcanii and a eubacterium Planococcus H8. H...
  3. Gutierrez M, Kamekura M, Holmes M, Dyall Smith M, Ventosa A. Taxonomic characterization of Haloferax sp. (" H. alicantei") strain Aa 2.2: description of Haloferax lucentensis sp. nov. Extremophiles. 2002;6:479-83 pubmed
    An extremely halophilic archaeon, previously named as Haloferax sp. strain Aa 2...
  4. Djeridi I, Militon C, Grossi V, Cuny P. Evidence for surfactant production by the haloarchaeon Haloferax sp. MSNC14 in hydrocarbon-containing media. Extremophiles. 2013;17:669-75 pubmed publisher
    The potential for surfactant production by the extreme halophilic archaeon Haloferax sp. MSNC14 in the presence of individual hydrocarbon substrates was studied...
  5. Konrad Z, Eichler J. Lipid modification of proteins in Archaea: attachment of a mevalonic acid-based lipid moiety to the surface-layer glycoprotein of Haloferax volcanii follows protein translocation. Biochem J. 2002;366:959-64 pubmed publisher
    Once the newly synthesized surface (S)-layer glycoprotein of the halophilic archaeaon Haloferax volcanii has traversed the plasma membrane, the protein undergoes a membrane-related, Mg(2+)-dependent maturation event, revealed as an ..
  6. Eichler J. Post-translational modification of the S-layer glycoprotein occurs following translocation across the plasma membrane of the haloarchaeon Haloferax volcanii. Eur J Biochem. 2001;268:4366-73 pubmed
    The halophilic archaeon Haloferax volcanii is surrounded by a protein shell solely comprised of the S-layer glycoprotein...
  7. Gebetsberger J, Wyss L, Mleczko A, Reuther J, Polacek N. A tRNA-derived fragment competes with mRNA for ribosome binding and regulates translation during stress. RNA Biol. 2017;14:1364-1373 pubmed publisher
    ..tRNA-derived fragment (Val-tRF) that is produced under certain stress conditions in the halophilic archaeon Haloferax volcanii is capable of binding to the small ribosomal subunit...
  8. Maslov I, Bogorodskiy A, Mishin A, Okhrimenko I, Gushchin I, Kalenov S, et al. Efficient non-cytotoxic fluorescent staining of halophiles. Sci Rep. 2018;8:2549 pubmed publisher
    ..The suggested dyes were tested on three archaeal (Hbt. salinarum, Haloferax sp., Halorubrum sp.) and two bacterial (Salicola sp., Halomonas sp.) strains of halophilic microorganisms...
  9. Stachler A, Marchfelder A. Gene Repression in Haloarchaea Using the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas I-B System. J Biol Chem. 2016;291:15226-42 pubmed publisher
    ..Here we use the model archaeon Haloferax volcanii to demonstrate that its endogenous CRISPR-Cas system I-B can be harnessed to repress gene expression in ..
  10. Abdul Halim M, Karch K, Zhou Y, Haft D, Garcia B, Pohlschröder M. Permuting the PGF Signature Motif Blocks both Archaeosortase-Dependent C-Terminal Cleavage and Prenyl Lipid Attachment for the Haloferax volcanii S-Layer Glycoprotein. J Bacteriol. 2015;198:808-15 pubmed publisher
    ..Recently, however, we demonstrated that the Haloferax volcanii SLG C terminus is removed by an archaeosortase (ArtA), a novel peptidase...
  11. Chen C, Hsu S, Lin M, Hsu Y. Mass production of C50 carotenoids by Haloferax mediterranei in using extruded rice bran and starch under optimal conductivity of brined medium. Bioprocess Biosyst Eng. 2015;38:2361-7 pubmed publisher
    Microbial carotenoids have potentially healthcare or medical applications. Haloferax mediterranei was difficult to economically grow into a large quantities as well as producing a valuable pigment of carotenoids...
  12. Rodrigo Baños M, Garbayo I, Vílchez C, Bonete M, Martínez Espinosa R. Carotenoids from Haloarchaea and Their Potential in Biotechnology. Mar Drugs. 2015;13:5508-32 pubmed publisher
    ..In particular, new scientific evidence of improved carotenoid production by one of the better known haloarchaeon (Haloferax mediterranei) is also discussed.
  13. Miranda H, Antelmann H, Hepowit N, Chavarria N, Krause D, Pritz J, et al. Archaeal ubiquitin-like SAMP3 is isopeptide-linked to proteins via a UbaA-dependent mechanism. Mol Cell Proteomics. 2014;13:220-39 pubmed publisher
    ..as protein modifiers and are required for the production of sulfur-containing biomolecules in the archaeon Haloferax volcanii...
  14. Salgaonkar B, Mani K, Nair A, Gangadharan S, Braganca J. Interspecific Interactions Among Members of Family Halobacteriaceae from Natural Solar Salterns. Probiotics Antimicrob Proteins. 2012;4:98-107 pubmed publisher
    ..culturable haloarchaeal isolates were obtained from solar salterns of Goa, which were grouped under Halococcus, Haloferax, Haloarcula and Halorubrum...
  15. Hattori T, Shiba H, Ashiki K, Araki T, Nagashima Y, Yoshimatsu K, et al. Anaerobic Growth of Haloarchaeon Haloferax volcanii by Denitrification Is Controlled by the Transcription Regulator NarO. J Bacteriol. 2016;198:1077-86 pubmed publisher
    The extremely halophilic archaeon Haloferax volcanii grows anaerobically by denitrification. A putative DNA-binding protein, NarO, is encoded upstream of the respiratory nitrate reductase gene of H. volcanii...
  16. Qi Q, Ito Y, Yoshimatsu K, Fujiwara T. Transcriptional regulation of dimethyl sulfoxide respiration in a haloarchaeon, Haloferax volcanii. Extremophiles. 2016;20:27-36 pubmed publisher
    The halophilic euryarchaeon Haloferax volcanii can grow anaerobically by DMSO respiration. DMSO reductase was induced by DMSO respiration not only under anaerobic growth conditions but also in denitrifying cells of H. volcanii...
  17. McMillan L, Hwang S, Farah R, Koh J, Chen S, Maupin Furlow J. Multiplex quantitative SILAC for analysis of archaeal proteomes: a case study of oxidative stress responses. Environ Microbiol. 2017;: pubmed publisher
    ..Here we report SILAC for quantitative comparison of archaeal proteomes, using Haloferax volcanii as a model...
  18. Naor A, Altman Price N, Soucy S, Green A, Mitiagin Y, Turgeman Grott I, et al. Impact of a homing intein on recombination frequency and organismal fitness. Proc Natl Acad Sci U S A. 2016;113:E4654-61 pubmed publisher
    ..Using the natural cell fusion ability of the halophilic archaeon Haloferax volcanii we were able to examine this question in vivo, by mating polB intein-positive [insertion site c in the ..
  19. Kılıç V, Kiliç G, Kutlu H, Martinez Espinosa R. Nitrate reduction in Haloferax alexandrinus: the case of assimilatory nitrate reductase. Extremophiles. 2017;21:551-561 pubmed publisher
    b>Haloferax alexandrinus Strain TM JCM 10717T?=?IFO 16590T is an extreme halophilic archaeon able to produce significant amounts of canthaxanthin...
  20. Pinto L, D Alincourt Carvalho Assef A, Vieira R, Clementino M, Albano R. Complete genome sequence of Haloferax gibbonsii strain ARA6, a potential producer of polyhydroxyalkanoates and halocins isolated from Araruama, Rio de Janeiro, Brasil. J Biotechnol. 2015;212:69-70 pubmed publisher
    b>Haloferax gibbonsii strain ARA6 is a haloarchaea isolated from saline saltern samples from Vermelha lake, located in Araruama region, Rio de Janeiro, Brazil...
  21. Gelsinger D, DiRuggiero J. Transcriptional Landscape and Regulatory Roles of Small Noncoding RNAs in the Oxidative Stress Response of the Haloarchaeon Haloferax volcanii. J Bacteriol. 2018;200: pubmed publisher
    ..and functional roles of sRNAs in the regulation of the oxidative stress response of the model haloarchaeon Haloferax volcanii Thousands of sRNAs, both intergenic and antisense, were discovered using strand-specific sRNA ..
  22. Pickl A, Johnsen U, Archer R, Schönheit P. Identification and characterization of 2-keto-3-deoxygluconate kinase and 2-keto-3-deoxygalactonate kinase in the haloarchaeon Haloferax volcanii. FEMS Microbiol Lett. 2014;361:76-83 pubmed
    The halophilic archaeon Haloferax volcanii has been proposed to degrade glucose via the semi-phosphorylative Entner-Doudoroff pathway, involving 2-keto-3-deoxygluconate kinase (KDGK) as key enzyme...
  23. Lestini R, Delpech F, Myllykallio H. DNA replication restart and cellular dynamics of Hef helicase/nuclease protein in Haloferax volcanii. Biochimie. 2015;118:254-63 pubmed publisher
    ..of a functional Hef::GFP (Green Fluorescent Protein) fusion protein in the living cells of halophilic archaea Haloferax volcanii...
  24. Peck R, PleÅŸa A, Graham S, Angelini D, Shaw E. Opsin-Mediated Inhibition of Bacterioruberin Synthesis in Halophilic Archaea. J Bacteriol. 2017;199: pubmed publisher
    ..In this work, we used heterologous expression in a related halophilic archaeon, Haloferax volcanii, to demonstrate that BO is sufficient to inhibit bacterioruberin synthesis catalyzed by the H...
  25. Pais J, Serafim L, Freitas F, Reis M. Conversion of cheese whey into poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Haloferax mediterranei. N Biotechnol. 2016;33:224-30 pubmed publisher
    b>Haloferax mediterranei was cultivated in highly saline medium using cheese whey as the substrate for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV)...
  26. Giroux X, MacNeill S. Inhibiting NAD+-dependent DNA ligase activity with 2-(cyclopentyloxy)-5'-deoxyadenosine (CPOdA) offers a new tool for DNA replication and repair studies in the model archaeon Haloferax volcanii. FEMS Microbiol Lett. 2015;362: pubmed publisher
    ..The haloarchaeal organism Haloferax volcanii encodes both ATP- and NAD(+)-dependent DNA ligase enzymes designated LigA and LigN, respectively...
  27. Kumar V, Saxena J, Tiwari S. Description of a halocin-producing Haloferax larsenii HA1 isolated from Pachpadra salt lake in Rajasthan. Arch Microbiol. 2016;198:181-92 pubmed publisher
    ..Identification of Haloferax larsenii strain HA1 was carried out using biochemical and molecular methods...
  28. Liu G, Cai S, Hou J, Zhao D, Han J, Zhou J, et al. Enoyl-CoA hydratase mediates polyhydroxyalkanoate mobilization in Haloferax mediterranei. Sci Rep. 2016;6:24015 pubmed publisher
    ..In this study, the functions of five putative (R)-specific enoyl-CoA hydratases (R-ECHs) in Haloferax mediterranei, named PhaJ1 to PhaJ5, respectively, were thoroughly investigated...
  29. Kandiba L, Eichler J. AglM and VNG1048G, Two Haloarchaeal UDP-Glucose Dehydrogenases, Show Different Salt-Related Behaviors. Life (Basel). 2016;6: pubmed publisher
    b>Haloferax volcanii AglM and Halobacterium salinarum VNG1048G are UDP-glucose dehydrogenases involved in N-glycosylation in each species...
  30. Cassidy J, Bruen L, Rosini E, Molla G, Pollegioni L, Paradisi F. Engineering substrate promiscuity in halophilic alcohol dehydrogenase (HvADH2) by in silico design. PLoS ONE. 2017;12:e0187482 pubmed publisher
    An alcohol dehydrogenase from the halophilic archaeon Haloferax volcanii (HvADH2) has been engineered by rational design to broaden its substrate scope towards the conversion of a range of aromatic substrates, including flurbiprofenol, ..
  31. Kiljunen S, Pajunen M, Savilahti H. Transposon Insertion Mutagenesis for Archaeal Gene Discovery. Methods Mol Biol. 2017;1498:309-320 pubmed
    ..behind various biological functions, and such a methodology is described in this chapter for a model archaeon Haloferax volcanii...
  32. Abdul Halim M, Stoltzfus J, Schulze S, Hippler M, Pohlschröder M. ArtA-Dependent Processing of a Tat Substrate Containing a Conserved Tripartite Structure That Is Not Localized at the C Terminus. J Bacteriol. 2017;199: pubmed publisher
    ..The extensively studied S-layer glycoprotein (SLG) of Haloferax volcanii, previously thought to be anchored via C-terminal intercalation into the membrane, was recently ..
  33. Alsafadi D, Alsalman S, Paradisi F. Extreme halophilic alcohol dehydrogenase mediated highly efficient syntheses of enantiopure aromatic alcohols. Org Biomol Chem. 2017;15:9169-9175 pubmed publisher
    ..hetero-aromatic and bicyclic structures) was carried out using halophilic alcohol dehydrogenase ADH2 from Haloferax volcanii (HvADH2). This enzyme showed an unprecedented substrate scope and absolute enatioselectivity...
  34. Patoli B, Winter J, Patoli A, Delahay R, Bunting K. Co-expression and purification of the RadA recombinase with the RadB paralog from Haloferax volcanii yields heteromeric ring-like structures. Microbiology. 2017;: pubmed publisher
    ..DNA recombination, replication and repair proteins of the halophilic euryarchaeon, Haloferax volcanii (Hvo) are of particular interest as they tend to resemble eukaryotic counterparts in both structure and ..
  35. Tamir A, Eichler J. N-Glycosylation Is Important for Proper Haloferax volcanii S-Layer Stability and Function. Appl Environ Microbiol. 2017;83: pubmed publisher
    ..Here, the impact of perturbed N-glycosylation on the structure and physiology of the haloarchaeon Haloferax volcanii was considered...
  36. Maurer S, Ludt K, Soppa J. Characterization of Copy Number Control of Two Haloferax volcanii Replication Origins Using Deletion Mutants and Haloarchaeal Artificial Chromosomes. J Bacteriol. 2018;200: pubmed publisher
    i>Haloferax volcanii is polyploid and contains about 20 genome copies under optimal conditions...
  37. Hepowit N, de Vera I, Cao S, Fu X, Wu Y, Uthandi S, et al. Mechanistic insight into protein modification and sulfur mobilization activities of noncanonical E1 and associated ubiquitin-like proteins of Archaea. FEBS J. 2016;283:3567-3586 pubmed publisher
    ..the mechanism of association of the Ubl protein with its cognate E1-like enzyme (SAMP and UbaA of the archaeon Haloferax volcanii, respectively)...
  38. Legerme G, Yang E, Esquivel R, Kiljunen S, Savilahti H, Pohlschröder M. Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants. Life (Basel). 2016;6: pubmed
    ..Therefore, we screened a Haloferax volcanii transposon insertion library for motility mutants using motility plates and adhesion mutants, using ..
  39. Wardell K, Haldenby S, Jones N, Liddell S, Ngo G, Allers T. RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator. DNA Repair (Amst). 2017;55:7-16 pubmed publisher
    ..In this study, we use the archaeon Haloferax volcanii to provide evidence to support this hypothesis...
  40. Wanner C, Soppa J. Genetic identification of three ABC transporters as essential elements for nitrate respiration in Haloferax volcanii. Genetics. 1999;152:1417-28 pubmed
    More than 40 nitrate respiration-deficient mutants of Haloferax volcanii belonging to three different phenotypic classes were isolated. All 15 mutants of the null phenotype were complemented with a genomic library of the wild type...
  41. Schavemaker P, Śmigiel W, Poolman B. Ribosome surface properties may impose limits on the nature of the cytoplasmic proteome. elife. 2017;6: pubmed publisher
    ..diffusion coefficients (D) in the cytoplasm of Escherichia coli, Lactococcus lactis and Haloferax volcanii. We find that in E...
  42. Ding Y, Vrionis H, Schneider J, Berezuk A, Khursigara C, Jarrell K. Complementation of an aglB Mutant of Methanococcus maripaludis with Heterologous Oligosaccharyltransferases. PLoS ONE. 2016;11:e0167611 pubmed publisher
    ..Attempts to use AglB from Methanocaldococcus jannaschii, Haloferax volcanii or Sulfolobus acidocaldarius to functionally replace the oligosaccharyltransferase activity missing in ..
  43. Babski J, Haas K, Näther Schindler D, Pfeiffer F, Förstner K, Hammelmann M, et al. Genome-wide identification of transcriptional start sites in the haloarchaeon Haloferax volcanii based on differential RNA-Seq (dRNA-Seq). BMC Genomics. 2016;17:629 pubmed publisher
    ..Therefore, we used dRNA-Seq to characterize the primary transcriptome of the model archaeon Haloferax volcanii. Three independent cultures of Hfx...
  44. Markov A, Kaznacheev I. Evolutionary consequences of polyploidy in prokaryotes and the origin of mitosis and meiosis. Biol Direct. 2016;11:28 pubmed publisher
    ..The mating system of the halophilic archaeon Haloferax volcanii probably represents an intermediate stage between typical prokaryotic and eukaryotic sex. H...
  45. Stantial N, Dumpe J, Pietrosimone K, Baltazar F, Crowley D. Transcription-coupled repair of UV damage in the halophilic archaea. DNA Repair (Amst). 2016;41:63-68 pubmed publisher
    ..We used Halobacterium sp. NRC-1 and Haloferax volcanii to determine if TCR occurs in the halophilic archaea...
  46. Mazguene S, Rossi M, Gogliettino M, Palmieri G, Cocca E, Mirino S, et al. Isolation and characterization from solar salterns of North Algeria of a haloarchaeon producing a new halocin. Extremophiles. 2018;22:259-270 pubmed publisher
    ..the basis of the partial 16S rRNA sequencing, the most efficient halocin producer was recognized as belonging to Haloferax (Hfx) sp...
  47. Hou J, Cui H. In Vitro Antioxidant, Antihemolytic, and Anticancer Activity of the Carotenoids from Halophilic Archaea. Curr Microbiol. 2018;75:266-271 pubmed publisher
    ..Halogeometricum limi, Haladaptatus litoreus, Haloplanus vescus, Halopelagius inordinatus, Halogranum rubrum, and Haloferax volcanii were identified by ultraviolet/visible spectroscopy, thin-layer chromatography, and high-performance ..
  48. Ferre Guell A, Winterburn J. Production of the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with varied composition using different nitrogen sources with Haloferax mediterranei. Extremophiles. 2017;21:1037-1047 pubmed publisher
    The extreme haloarchaea Haloferax mediterranei accumulates poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) without the need for specific precursors...
  49. Ausiannikava D, Allers T. Diversity of DNA Replication in the Archaea. Genes (Basel). 2017;8: pubmed publisher
    ..This poses a challenge to the regulation of DNA replication. However, one archaeal species (Haloferax volcanii) can survive without replication origins; instead, it uses homologous recombination as an alternative ..
  50. Gregor D, Pfeifer F. Use of a halobacterial bgaH reporter gene to analyse the regulation of gene expression in halophilic archaea. Microbiology. 2001;147:1745-54 pubmed
    The bgaH reading frame encoding a beta-galactosidase of 'Haloferax alicantei' was used as a reporter gene to investigate three different promoter regions derived from gvpA genes of Haloferax mediterranei (mc-gvpA) and Halobacterium ..
  51. Anton J, Llobet Brossa E, Rodriguez Valera F, Amann R. Fluorescence in situ hybridization analysis of the prokaryotic community inhabiting crystallizer ponds. Environ Microbiol. 1999;1:517-23 pubmed
    ..Additionally, FISH with a probe targeted to the genus Haloarcula, members of which are frequently isolated from this environment, indicated that this genus accounts for less than 0.1% of the total prokaryotic community...
  52. Fu W, Oriel P. Degradation of 3-phenylpropionic acid by Haloferax sp. D1227. Extremophiles. 1999;3:45-53 pubmed
    b>Haloferax sp. D1227, isolated from soil contaminated with highly saline oil brine, is the first halophilic archaeon to demonstrate the utilization of aromatic compounds (i.e...
  53. Salgaonkar B, Mani K, Bragança J. Accumulation of polyhydroxyalkanoates by halophilic archaea isolated from traditional solar salterns of India. Extremophiles. 2013;17:787-95 pubmed publisher
    ..and genotypic characterization the cultures TN4, TN5, TN6, TN7, TN10 and BBK2 were grouped under genus Haloferax whereas isolate TN9 was grouped under the genus Halogeometricum...
  54. Chen S, Wang C, Xiang H. Sequence analysis and minimal replicon determination of a new haloarchaeal plasmid pHF2 isolated from Haloferax sp. strain Q22. Plasmid. 2016;83:1-7 pubmed publisher
    A new cryptic plasmid, pHF2 (2520 bp), was isolated from Haloferax sp. strain Q22 (CGMCC 1.15317), a haloarchaeal strain living in a subterranean halite deposit...
  55. Asker D, Ohta Y. Production of canthaxanthin by Haloferax alexandrinus under non-aseptic conditions and a simple, rapid method for its extraction. Appl Microbiol Biotechnol. 2002;58:743-50 pubmed
    The production of carotenoids from Haloferax alexandrinus strain TM(T) was investigated at various concentrations of NaCl (10-25%) in culture media under non-aseptic conditions...
  56. Asker D, Ohta Y. PCR and blot hybridization for rapid identification of Haloferax species. Can J Microbiol. 2002;48:468-72 pubmed
    Based on the amplification of a 16S rDNA, a PCR assay for the identification of species of Haloferax to genus level was performed. Two variable regions of the 16S rDNA in Haloferax spp...
  57. Xu X, Wu Y, Wang C, Oren A, Zhou P, Wu M. Haloferax larsenii sp. nov., an extremely halophilic archaeon from a solar saltern. Int J Syst Evol Microbiol. 2007;57:717-20 pubmed
    ..Comparative 16S rRNA gene analysis revealed 96.4-97.4 % sequence similarity to members of the genus Haloferax. The major polar lipids were C(20)C(20) derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate ..
  58. Fischer S, Maier L, Stoll B, Brendel J, Fischer E, Pfeiffer F, et al. An archaeal immune system can detect multiple protospacer adjacent motifs (PAMs) to target invader DNA. J Biol Chem. 2012;287:33351-63 pubmed
    ..Here, we analyzed the CRISPR/Cas system of Haloferax volcanii using a plasmid-based invader assay...
  59. Lynch E, Langille M, Darling A, Wilbanks E, Haltiner C, Shao K, et al. Sequencing of seven haloarchaeal genomes reveals patterns of genomic flux. PLoS ONE. 2012;7:e41389 pubmed publisher
    We report the sequencing of seven genomes from two haloarchaeal genera, Haloferax and Haloarcula...
  60. Maier L, Stoll B, Brendel J, Fischer S, Pfeiffer F, Dyall Smith M, et al. The ring of confidence: a haloarchaeal CRISPR/Cas system. Biochem Soc Trans. 2013;41:374-8 pubmed publisher
    ..In the present paper, we summarize our current knowledge about the CRISPR/Cas system in Haloferax volcanii, an extremely halophilic archaeon that was isolated from the Dead Sea. H...
  61. Saralov A, Baslerov R, Kuznetsov B. Haloferax chudinovii sp. nov., a halophilic archaeon from Permian potassium salt deposits. Extremophiles. 2013;17:499-504 pubmed publisher
    ..0-65.0 mol %. Phylogenetic analysis of the 16S rRNA gene sequences showed high similarity of the new strains to Haloferax species: H. denitrificans (99.2%) and H. volcanii (99.1%), H. larsenii (96.9%) and H. elongans (96.6%)...
  62. Naor A, Thiaville P, Altman Price N, Cohen Or I, Allers T, De Crecy Lagard V, et al. A genetic investigation of the KEOPS complex in halophilic Archaea. PLoS ONE. 2012;7:e43013 pubmed publisher
    ..we attempted to delete the genes encoding the four conserved subunits of the KEOPS complex in the euryarchaeote Haloferax volcanii and study their phenotypes in vivo...
  63. Chen S, Tulloss R, Liu Y, Feng B, Zhao Z, Yang Z. Lateral gene transfer occurring in haloarchaea: an interpretative imitation study. World J Microbiol Biotechnol. 2012;28:2913-8 pubmed publisher
    ..In this study, LGT was induced in two genera of haloarchaea, Haloferax and Halorubrum, by modification of salt concentration of media-a factor that may vary naturally in native ..
  64. Cui H, Gao X, Sun F, Dong Y, Xu X, Zhou Y, et al. Halogranum rubrum gen. nov., sp. nov., a halophilic archaeon isolated from a marine solar saltern. Int J Syst Evol Microbiol. 2010;60:1366-71 pubmed publisher
    ..3 % and highest sequence similarity with the closest relative (Haloferax larsenii) was 91.4 %...
  65. Arahal D, Gutierrez M, Volcani B, Ventosa A. Taxonomic analysis of extremely halophilic archaea isolated from 56-years-old dead sea brine samples. Syst Appl Microbiol. 2000;23:376-85 pubmed
    ..These groups were found to represent some members of three different genera - Haloarcula (phenons A, B, and C), Haloferax (phenons D and E) and Halobacterium (phenon F) - of the family Halobacteriaceae, some of them never reported to ..
  66. Allen M, Goh F, Leuko S, Echigo A, Mizuki T, Usami R, et al. Haloferax elongans sp. nov. and Haloferax mucosum sp. nov., isolated from microbial mats from Hamelin Pool, Shark Bay, Australia. Int J Syst Evol Microbiol. 2008;58:798-802 pubmed publisher
    ..and 16S rRNA gene sequence analysis indicated that all strains were most closely related to members of the genus Haloferax (98.1-99.4 % similarity)...
  67. Tindall B, Tomlinson G, Hochstein L. Transfer of Halobacterium denitrificans (Tomlinson, Jahnke, and Hochstein) to the genus Haloferax as Haloferax denitrificans comb. nov. Int J Syst Bacteriol. 1989;39:359-60 pubmed
    ..data, this organism exhibits features which indicate that it is more closely related to members of the genus Haloferax. On the basis of such criteria, we propose that Halobacterium denitrificans be reclassified as Haloferax ..
  68. Enache M, Itoh T, Kamekura M, Teodosiu G, Dumitru L. Haloferax prahovense sp. nov., an extremely halophilic archaeon isolated from a Romanian salt lake. Int J Syst Evol Microbiol. 2007;57:393-7 pubmed
    ..7 mol%. The 16S rRNA gene sequence of the novel strain was most closely related to species of the genus Haloferax (97.3-99.3 % sequence similarity)...
  69. Okeke B, Giblin T, Frankenberger W. Reduction of perchlorate and nitrate by salt tolerant bacteria. Environ Pollut. 2002;118:357-63 pubmed
    ..Salt tolerant bacterial isolates from the enrichment cultures and two denitrifying salt tolerant bacteria, Haloferax denitrificans and Parococcus halodenitricans, substantially reduced perchlorate...
  70. Asker D, Ohta Y. Haloferax alexandrinus sp. nov., an extremely halophilic canthaxanthin-producing archaeon from a solar saltern in Alexandria (Egypt). Int J Syst Evol Microbiol. 2002;52:729-38 pubmed
    ..5+/-0.3 mol %. On the basis of polar lipid analysis, the isolate belonged to the genus Haloferax. Analysis of the 16S rDNA sequence showed the highest similarity (>99%) to be to the type strain Haloferax ..
  71. Rodriguez Fonseca C, Phan H, Long K, Porse B, Kirillov S, Amils R, et al. Puromycin-rRNA interaction sites at the peptidyl transferase center. RNA. 2000;6:744-54 pubmed
    ..center of ribosomes from Escherichia coli and of puromycin-hypersensitive ribosomes from the archaeon Haloferax gibbonsii...
  72. Nuttall S, Deutschel S, Irving R, Serrano Gomicia J, Dyall Smith M. The ShBle resistance determinant from Streptoalloteichus hindustanus is expressed in Haloferax volcanii and confers resistance to bleomycin. Biochem J. 2000;346 Pt 2:251-4 pubmed
    ..bleomycin-resistance protein from Streptoalloteichus hindustanus (ShBle) in the extremely halophilic archaeon Haloferax volcanii, and shown that transformed haloarchaea are resistant to bleomycin...
  73. Lange C, Zaigler A, Hammelmann M, Twellmeyer J, Raddatz G, Schuster S, et al. Genome-wide analysis of growth phase-dependent translational and transcriptional regulation in halophilic archaea. BMC Genomics. 2007;8:415 pubmed
    ..Additional species have not been investigated yet. Particularly, until now no global study of translational control with any prokaryotic species was available...
  74. Fairley D, Wang G, Rensing C, Pepper I, Larkin M. Expression of gentisate 1,2-dioxygenase (gdoA) genes involved in aromatic degradation in two haloarchaeal genera. Appl Microbiol Biotechnol. 2006;73:691-5 pubmed
    ..In contrast, gdoA was expressed in Haloferax sp...
  75. McDuff S, King G, Neupane S, Myers M. Isolation and characterization of extremely halophilic CO-oxidizing Euryarchaeota from hypersaline cinders, sediments and soils and description of a novel CO oxidizer, Haloferax namakaokahaiae Mke2.3T, sp. nov. FEMS Microbiol Ecol. 2016;92:fiw028 pubmed publisher
    ..A halophilic euryarchaeote, Haloferax strain Mke2.3(T), was isolated from Hawai'i Island saline cinders. Haloferax strain Mke2...
  76. Mayrhofer Iro M, Ladurner A, Meissner C, Derntl C, Reiter M, Haider F, et al. Utilization of virus ?Ch1 elements to establish a shuttle vector system for Halo(alkali)philic Archaea via transformation of Natrialba magadii. Appl Environ Microbiol. 2013;79:2741-8 pubmed publisher
    ..exemplified by the expression of Ch1 ORF3452, which encodes a structural protein, and the reporter gene bgaH of Haloferax lucentense in N. magadii. The new transformation/vector system will facilitate genetic studies within N...
  77. Pérez Pomares F, Diaz S, Bautista V, Pire C, Bravo G, Esclapez J, et al. Identification of several intracellular carbohydrate-degrading activities from the halophilic archaeon Haloferax mediterranei. Extremophiles. 2009;13:633-41 pubmed publisher
    ..activities, designated AMY1, AMY2, and AMY3 were detected in the cytoplasm of the extreme halophilic archaeon Haloferax mediterranei grown in a starch containing medium...
  78. Tapilatu Y, Grossi V, Acquaviva M, Militon C, Bertrand J, Cuny P. Isolation of hydrocarbon-degrading extremely halophilic archaea from an uncontaminated hypersaline pond (Camargue, France). Extremophiles. 2010;14:225-31 pubmed publisher
    ..one (strain MSNC 2) was closely related to Haloarcula and three (strains MSNC 4, MSNC 14, and MSNC 16) to Haloferax. Biodegradation assays showed that depending on the strain, 32 to 95% (0...
  79. Holmes M, Dyall Smith M. Sequence and expression of a halobacterial beta-galactosidase gene. Mol Microbiol. 2000;36:114-22 pubmed
    ..In a previous study, a beta-galactosidase from Haloferax alicantei was purified and several peptide sequences determined...
  80. Offner S, Ziese U, Wanner G, Typke D, Pfeifer F. Structural characteristics of halobacterial gas vesicles. Microbiology. 1998;144 ( Pt 5):1331-42 pubmed
    ..salinarum PHH4 and the single chromosomal mc-vac region of Haloferax mediterranei...