halobacterium salinarum

Summary

Summary: A species of halophilic archaea found in salt lakes. Some strains form a PURPLE MEMBRANE under anaerobic conditions.

Top Publications

  1. Kixmüller D, Greie J. Construction and characterization of a gradually inducible expression vector for Halobacterium salinarum, based on the kdp promoter. Appl Environ Microbiol. 2012;78:2100-5 pubmed publisher
    ..For Halobacterium salinarum, which is often used as a paradigm for halophilic archaea, such an inducible expression system is not ..
  2. Mormile M, Biesen M, Gutierrez M, Ventosa A, Pavlovich J, Onstott T, et al. Isolation of Halobacterium salinarum retrieved directly from halite brine inclusions. Environ Microbiol. 2003;5:1094-102 pubmed
    ..analysis (16S rDNA) of the 97 kyr isolate, designated BBH 001, revealed a homology of 100% with Halobacterium salinarum. DNA-DNA hybridization experiments confirmed that BBH 001 was closely related to H...
  3. del Rosario R, Diener F, Diener M, Oesterhelt D. The steady-state phase distribution of the motor switch complex model of Halobacterium salinarum. Math Biosci. 2009;222:117-26 pubmed publisher
    Steady-state analysis is performed on the kinetic model for the switch complex of the flagellar motor of Halobacterium salinarum (Nutsch et al.)...
  4. Koch M, Oesterhelt D. MpcT is the transducer for membrane potential changes in Halobacterium salinarum. Mol Microbiol. 2005;55:1681-94 pubmed
    In Halobacterium salinarum mutants containing either of the light-driven ion pumps bacteriorhodopsin (H(+)) or halorhodopsin (Cl(-)) as their only retinal protein, a decrease of irradiance in the absence of respiration causes a ..
  5. Lange C, Zaigler A, Hammelmann M, Twellmeyer J, Raddatz G, Schuster S, et al. Genome-wide analysis of growth phase-dependent translational and transcriptional regulation in halophilic archaea. BMC Genomics. 2007;8:415 pubmed
    ..Additional species have not been investigated yet. Particularly, until now no global study of translational control with any prokaryotic species was available...
  6. Engel M, Catchpole H. A microprobe analysis of inorganic elements in Halobacterium salinarum. Cell Biol Int. 2005;29:616-22 pubmed
    b>Halobacterium salinarum were grown on peptone agar containing 4.28 M NaCl, 0.036 M K and other salts...
  7. Streif S, Staudinger W, Marwan W, Oesterhelt D. Flagellar rotation in the archaeon Halobacterium salinarum depends on ATP. J Mol Biol. 2008;384:1-8 pubmed publisher
    b>Halobacterium salinarum swims with the help of a polarly inserted flagellar bundle. In energized cells, the flagellar motors rotate continuously, occasionally switching the rotational sense...
  8. Li R, Cui X, Hu W, Lu Z, Li C. Fabrication of oriented poly-L-lysine/bacteriorhodopsin-embedded purple membrane multilayer structure for enhanced photoelectric response. J Colloid Interface Sci. 2010;344:150-7 pubmed publisher
    ..The photocurrent of bR-PM from the film through PLL assembler is higher than those assembled by other polycations, thus rendering a new platform to effectively enhance the bR photoelectric responses...
  9. Kish A, Griffin P, Rogers K, Fogel M, Hemley R, Steele A. High-pressure tolerance in Halobacterium salinarum NRC-1 and other non-piezophilic prokaryotes. Extremophiles. 2012;16:355-61 pubmed publisher
    ..The pressure survivals of Halobacterium salinarum NRC-1, Deinococcus radiodurans R1, and Chromohalobacter salexigens were compared to that of Escherichia ..

More Information

Publications104 found, 100 shown here

  1. Beznosov S, Piatibratov M, Fedorov O. [Multicomponent nature of Halobacterium salinarum flagella]. Mikrobiologiia. 2007;76:494-501 pubmed
    Filaments of the flagellum of the halophilic archaeon Halobacterium salinarum consist of five flagellins: A1, A2, B1, B2, and B3, which are encoded by five genes localized in tandem in twoflgA and flgB operons...
  2. Weisel J, Wagner S, Klug G. The Nop5-L7A-fibrillarin RNP complex and a novel box C/D containing sRNA of Halobacterium salinarum NRC-1. Biochem Biophys Res Commun. 2010;394:542-7 pubmed publisher
    ..To analyze the putative box C/D RNP complex in the extremely halophilic Halobacterium salinarum NRC-1 we performed pull-down analysis and identified the proteins Nop5, L7A, and fibrillarin and the tRNA(..
  3. Wende A, Furtwängler K, Oesterhelt D. Phosphate-dependent behavior of the archaeon Halobacterium salinarum strain R1. J Bacteriol. 2009;191:3852-60 pubmed publisher
    ..We conducted a microarray analysis to explore the phosphate-dependent gene expression of Halobacterium salinarum strain R1...
  4. Facciotti M, Pang W, Lo F, Whitehead K, Koide T, Masumura K, et al. Large scale physiological readjustment during growth enables rapid, comprehensive and inexpensive systems analysis. BMC Syst Biol. 2010;4:64 pubmed publisher
    ..We have discovered in the model organism Halobacterium salinarum NRC-1 that batch culturing in complex medium stimulates meaningful changes in the expression of ..
  5. Teufel K, Pfeifer F. Interaction of transcription activator GvpE with TATA-box-binding proteins of Halobacterium salinarum. Arch Microbiol. 2010;192:143-9 pubmed publisher
    ..volcanii. From these results, it appears that GvpE interacts with any TBP of Hbt. salinarum. This interaction might attract TBP and subsequently TFB and RNAP to the promoter and thus enhance transcription of the gvp gene cluster. ..
  6. Bieger B, Essen L, Oesterhelt D. Crystal structure of halophilic dodecin: a novel, dodecameric flavin binding protein from Halobacterium salinarum. Structure. 2003;11:375-85 pubmed
    A novel, 68 amino acid long flavoprotein called dodecin has been discovered in the proteome of Halobacterium salinarum by inverse structural genomics. The 1...
  7. Gregor D, Pfeifer F. In vivo analyses of constitutive and regulated promoters in halophilic archaea. Microbiology. 2005;151:25-33 pubmed
    The two gvpA promoters P(cA) and P(pA) of Halobacterium salinarum, and the P(mcA) promoter of Haloferax mediterranei were investigated with respect to growth-phase-dependent expression and regulation in Haloferax volcanii transformants ..
  8. Koide T, Reiss D, Bare J, Pang W, Facciotti M, Schmid A, et al. Prevalence of transcription promoters within archaeal operons and coding sequences. Mol Syst Biol. 2009;5:285 pubmed publisher
    ..of transcript structures of approximately 64% of all genes, including putative non-coding RNAs in Halobacterium salinarum NRC-1...
  9. Koch M, Staudinger W, Siedler F, Oesterhelt D. Physiological sites of deamidation and methyl esterification in sensory transducers of Halobacterium salinarum. J Mol Biol. 2008;380:285-302 pubmed publisher
    In Halobacterium salinarum, up to 18 sensory transducers (Htrs) relay environmental stimuli to an intracellular signaling system to induce tactic responses...
  10. Scheuch S, Marschaus L, Sartorius Neef S, Pfeifer F. Regulation of gvp genes encoding gas vesicle proteins in halophilic Archaea. Arch Microbiol. 2008;190:333-9 pubmed publisher
    Three gas vesicle gene clusters derived from Halobacterium salinarum (p-vac and c-vac) and Haloferax mediterranei (mc-vac) are used as model systems to study gene regulation in Archaea...
  11. Furtwängler K, Tarasov V, Wende A, Schwarz C, Oesterhelt D. Regulation of phosphate uptake via Pst transporters in Halobacterium salinarum R1. Mol Microbiol. 2010;76:378-92 pubmed publisher
    The genome of the archaeon Halobacterium salinarum contains two copies of the pst (phosphate-specific transport) operon, the genes of which are related to well-studied bacterial homologues...
  12. Yao A, Facciotti M. Regulatory multidimensionality of gas vesicle biogenesis in Halobacterium salinarum NRC-1. Archaea. 2011;2011:716456 pubmed publisher
    It is becoming clear that the regulation of gas vesicle biogenesis in Halobacterium salinarum NRC-1 is multifaceted and appears to integrate environmental and metabolic cues at both the transcriptional and posttranscriptional levels...
  13. Buchsteiner A, Lechner R, Hauss T, Dencher N. Relationship between structure, dynamics and function of hydrated purple membrane investigated by neutron scattering and dielectric spectroscopy. J Mol Biol. 2007;371:914-23 pubmed
    ..The dynamic transition in the vicinity of 180 K was shown to be necessary to ensure that the M1 intermediate can be populated and that the melting of crystallized bulk water above 255 K enables the completion of the photocycle...
  14. Kawakami Y, Hayashi N, Ema M, Nakayama M. Effects of divalent cations on Halobacterium salinarum cell aggregation. J Biosci Bioeng. 2007;104:42-6 pubmed
    Ca(2+) was found to be essential for initiating Halobacterium salinarum CCM 2090 cell aggregation. The floc formed from such aggregation could easily be dissociated without cellular lysis by sodium citrate...
  15. Schmid A, Reiss D, Kaur A, Pan M, King N, Van P, et al. The anatomy of microbial cell state transitions in response to oxygen. Genome Res. 2007;17:1399-413 pubmed
    ..Dynamic temporal analysis of relationships between transcription and translation of key genes suggests several important mechanisms for cellular sustenance under anoxia as well as specific instances of post-transcriptional regulation. ..
  16. Bisle B, Schmidt A, Scheibe B, Klein C, Tebbe A, Kellermann J, et al. Quantitative profiling of the membrane proteome in a halophilic archaeon. Mol Cell Proteomics. 2006;5:1543-58 pubmed
    We present a large scale quantitation study of the membrane proteome from Halobacterium salinarum. To overcome problems generally encountered with membrane proteins, we established a membrane preparation protocol that allows the ..
  17. Baumann A, Lange C, Soppa J. Transcriptome changes and cAMP oscillations in an archaeal cell cycle. BMC Cell Biol. 2007;8:21 pubmed
    ..A synchronization procedure for the archaeon Halobacterium salinarum was optimized, so that nearly 100% of all cells divide in a time interval that is 1/4th of the generation ..
  18. Tarasov V, Besir H, Schwaiger R, Klee K, Furtwängler K, Pfeiffer F, et al. A small protein from the bop-brp intergenic region of Halobacterium salinarum contains a zinc finger motif and regulates bop and crtB1 transcription. Mol Microbiol. 2008;67:772-80 pubmed publisher
    Bacteriorhodopsin, the photosynthetic protein of Halobacterium salinarum, is optimally expressed under anaerobic growth conditions...
  19. Mironova O, Efremov R, Person B, Heberle J, Budyak I, Buldt G, et al. Functional characterization of sensory rhodopsin II from Halobacterium salinarum expressed in Escherichia coli. FEBS Lett. 2005;579:3147-51 pubmed
    Sensory rhodopsin II (SRII) from Halobacterium salinarum is heterologously expressed in Escherichia coli with a yield of 3-4 mg of purified SRII per liter cell culture...
  20. Kamihira M, Watts A. Functionally relevant coupled dynamic profile of bacteriorhodopsin and lipids in purple membranes. Biochemistry. 2006;45:4304-13 pubmed publisher
    ..These results suggest that motions in the 10s micros correlation regime may be functionally important for the photocycle of bR, and protein-lipid interactions are motionally coupled in this dynamic regime...
  21. Sprott G, Dicaire C, Côté J, Whitfield D. Adjuvant potential of archaeal synthetic glycolipid mimetics critically depends on the glyco head group structure. Glycobiology. 2008;18:559-65 pubmed publisher
    ..This first study on synthetic archaeal lipid adjuvants reveals potential for this class of regulatory friendly, easily scalable, inexpensive, and potent glyco-adjuvant...
  22. Cohen Krausz S, Trachtenberg S. The structure of the archeabacterial flagellar filament of the extreme halophile Halobacterium salinarum R1M1 and its relation to eubacterial flagellar filaments and type IV pili. J Mol Biol. 2002;321:383-95 pubmed
    ..filaments, we determined the unique symmetry of the flagellar filament of the extreme halophile Halobacterium salinarum strain R1M1 and calculated a three-dimensional density map to a resolution of 19A...
  23. Whitfield D, Eichler E, Sprott G. Synthesis of archaeal glycolipid adjuvants-what is the optimum number of sugars?. Carbohydr Res. 2008;343:2349-60 pubmed publisher
    ..To generate the parent archaeol alcohol precursor, the polar lipids extracted from the archaeon Halobacterium salinarum were hydrolyzed to remove polar head groups, and the archaeol so generated partitioned into diethyl ether...
  24. Tarasov V, Schwaiger R, Furtw ngler K, Dyall Smith M, Oesterhelt D. A small basic protein from the brz-brb operon is involved in regulation of bop transcription in Halobacterium salinarum. BMC Mol Biol. 2011;12:42 pubmed publisher
    The halophilic archaeon Halobacterium salinarum expresses bacteriorhodopsin, a retinal-protein that allows photosynthetic growth...
  25. Aharoni A, Ottolenghi M, Sheves M. Retinal isomerization in bacteriorhodopsin is controlled by specific chromophore-protein interactions. A study with noncovalent artificial pigments. Biochemistry. 2001;40:13310-9 pubmed
    ..In contrast to the bound analogues, no photocycle could be detected. It is suggested that a specific retinal-protein geometrical arrangement in the binding site is a prerequisite for achieving the selective retinal photoisomerization. ..
  26. Perálvarez Marín A, Lorenz Fonfria V, Bourdelande J, Querol E, Kandori H, Padrós E. Inter-helical hydrogen bonds are essential elements for intra-protein signal transduction: the role of Asp115 in bacteriorhodopsin transport function. J Mol Biol. 2007;368:666-76 pubmed
    ..It also supports the idea that intra-helical hydrogen bonding clusters in the buried regions of transmembrane proteins can be potential elements in intra-protein signal transduction...
  27. Wang W, Knopf G, Bassi A. Photoelectric properties of a detector based on dried bacteriorhodopsin film. Biosens Bioelectron. 2006;21:1309-19 pubmed
    ..Experimental observations and a predictive model both support the notion that dried bR film can be used in simple highly sensitive photo-detector designs. ..
  28. Brenneis M, Hering O, Lange C, Soppa J. Experimental characterization of Cis-acting elements important for translation and transcription in halophilic archaea. PLoS Genet. 2007;3:e229 pubmed
    ..The 5'-ends and 3'-ends of 40 transcripts of two haloarchaeal species, Halobacterium salinarum and Haloferax volcanii, have been determined...
  29. Bleiholder A, Frommherz R, Teufel K, Pfeifer F. Expression of multiple tfb genes in different Halobacterium salinarum strains and interaction of TFB with transcriptional activator GvpE. Arch Microbiol. 2012;194:269-79 pubmed publisher
    b>Halobacterium salinarum NRC-1 contains multiple TBP and TFB proteins required for the recruitment of RNA polymerase for transcription initiation. The presence and the expression of genes encoding TFB were investigated in the two Hbt...
  30. Hubmacher D, Matzanke B, Anemüller S. Effects of iron limitation on the respiratory chain and the membrane cytochrome pattern of the Euryarchaeon Halobacterium salinarum. Biol Chem. 2003;384:1565-73 pubmed
    The effects of iron limitation on the electron transport chain of the extremely halophilic Euryarchaeon Halobacterium salinarum were analyzed...
  31. Zeth K, Offermann S, Essen L, Oesterhelt D. Iron-oxo clusters biomineralizing on protein surfaces: structural analysis of Halobacterium salinarum DpsA in its low- and high-iron states. Proc Natl Acad Sci U S A. 2004;101:13780-5 pubmed publisher
    ..of the Dps-like (Dps, DNA-protecting protein during starvation) ferritin protein DpsA from the halophile Halobacterium salinarum was determined with low endogenous iron content at 1.6-A resolution...
  32. Hubmacher D, Matzanke B, Anemüller S. Iron-uptake in the Euryarchaeon Halobacterium salinarum. Biometals. 2007;20:539-47 pubmed
    ..In this study, the mechanism of iron transport in the extremely halophilic Euryarchaeon Halobacterium salinarum strain JW 5 was analyzed...
  33. Nutsch T, Marwan W, Oesterhelt D, Gilles E. Signal processing and flagellar motor switching during phototaxis of Halobacterium salinarum. Genome Res. 2003;13:2406-12 pubmed
    ..In contrast to Escherichia coli, Halobacterium salinarum, in addition, responds to visible light, which is sensed through specific photoreceptors of different ..
  34. Hubmacher D, Matzanke B, Anemuller S. Investigations of iron uptake in Halobacterium salinarum. Biochem Soc Trans. 2002;30:710-2 pubmed
    The iron transport in the extremely halophilic Euryarchaeon Halobacterium salinarum JW5 was investigated. Experiments to detect endogenous siderophores from H. salinarum failed, but it was able to utilize exogenous siderophores...
  35. Cho C, Lee S, Choi J, Park S, Ha D, Kim H, et al. Improvement of the two-dimensional gel electrophoresis analysis for the proteome study of Halobacterium salinarum. Proteomics. 2003;3:2325-9 pubmed
    ..use of two-dimensional gel electrophoresis (2-DE) for sample preparation and separation of proteins from Halobacterium salinarum. In particular, proteins from cells grown in 25% NaCl are difficult to resolve by 2-DE due to the ..
  36. Sartorius Neef S, Pfeifer F. In vivo studies on putative Shine-Dalgarno sequences of the halophilic archaeon Halobacterium salinarum. Mol Microbiol. 2004;51:579-88 pubmed
    ..of mRNA in halophilic archaea was investigated for two gvp genes involved in gas vesicle formation in Halobacterium salinarum PHH1...
  37. Hundt S, Zaigler A, Lange C, Soppa J, Klug G. Global analysis of mRNA decay in Halobacterium salinarum NRC-1 at single-gene resolution using DNA microarrays. J Bacteriol. 2007;189:6936-44 pubmed
    ..to analyze mRNA degradation in exponentially growing cultures of the extremely halophilic euryarchaeon Halobacterium salinarum NRC-1 on a global level...
  38. Tebbe A, Klein C, Bisle B, Siedler F, Scheffer B, Garcia Rizo C, et al. Analysis of the cytosolic proteome of Halobacterium salinarum and its implication for genome annotation. Proteomics. 2005;5:168-79 pubmed
    The halophilic archaeon Halobacterium salinarum (strain R1, DSM 671) contains 2784 protein-coding genes as derived from the genome sequence...
  39. Klein C, Aivaliotis M, Olsen J, Falb M, Besir H, Scheffer B, et al. The low molecular weight proteome of Halobacterium salinarum. J Proteome Res. 2007;6:1510-8 pubmed
    Systematic investigation of low molecular weight proteins (LMW, below 20 kDa) in the archaeon Halobacterium salinarum resulted in a 6-fold enhancement of the identification rate, reaching 35% of the theoretical proteome in that size ..
  40. Van P, Schmid A, King N, Kaur A, Pan M, Whitehead K, et al. Halobacterium salinarum NRC-1 PeptideAtlas: toward strategies for targeted proteomics and improved proteome coverage. J Proteome Res. 2008;7:3755-64 pubmed publisher
    ..7% of the predicted proteome of the extremely halophilic archaeon Halobacterium salinarum NRC-1 by compiling approximately 636 000 tandem mass spectra from 497 mass spectrometry runs in 88 ..
  41. Wimmer F, Oberwinkler T, Bisle B, Tittor J, Oesterhelt D. Identification of the arginine/ornithine antiporter ArcD from Halobacterium salinarum. FEBS Lett. 2008;582:3771-5 pubmed publisher
    ..This clearly demonstrates that the purified 34kD protein is the functional unit...
  42. Whitehead K, Pan M, Masumura K, Bonneau R, Baliga N. Diurnally entrained anticipatory behavior in archaea. PLoS ONE. 2009;4:e5485 pubmed publisher
    ..LD)-entrained diurnal oscillatory transcription in up to 12% of all genes of a halophilic archaeon Halobacterium salinarum NRC-1...
  43. Liu W, Hanson M, Stevens R, Cherezov V. LCP-Tm: an assay to measure and understand stability of membrane proteins in a membrane environment. Biophys J. 2010;98:1539-48 pubmed publisher
    ..This assay has the potential to guide protein engineering efforts and identify stabilizing conditions that may improve the chances of obtaining high-resolution structures of intrinsically unstable membrane proteins...
  44. Kokoeva M, Storch K, Klein C, Oesterhelt D. A novel mode of sensory transduction in archaea: binding protein-mediated chemotaxis towards osmoprotectants and amino acids. EMBO J. 2002;21:2312-22 pubmed publisher
    Directly upstream of the Halobacterium salinarum transducer genes basT and htpIV we identified two open reading frames (orfs) with significant homologies to genes encoding binding proteins for amino acids and compatible solutes, ..
  45. Giladi M, Bitan Banin G, Mevarech M, Ortenberg R. Genetic evidence for a novel thymidylate synthase in the halophilic archaeon Halobacterium salinarum and in Campylobacter jejuni. FEMS Microbiol Lett. 2002;216:105-9 pubmed
    A search of the complete genome sequence of the halophilic archaeon Halobacterium salinarum failed to identify a gene homologous to the thymidylate synthase (thyA) gene present in the closely related Haloferax volcanii...
  46. Lobasso S, Lopalco P, Lattanzio V, Corcelli A. Osmotic shock induces the presence of glycocardiolipin in the purple membrane of Halobacterium salinarum. J Lipid Res. 2003;44:2120-6 pubmed
    In the purple membrane (PM) of Halobacterium salinarum is present a phospholipid dimer consisting of sulfo-triglycosyl-diether (S-TGD-1) esterified to the phosphate group of phosphatidic acid (PA), i.e...
  47. Lee M, Joo W, Kim C. Identification of a novel protein D3UPCA from Halobacterium salinarum and prediction of its function. Proteomics. 2004;4:3622-31 pubmed
    b>Halobacterium salinarum is an extremely halophilic archaea, which is able to live in highly saline environments...
  48. Weik M, Lehnert U, Zaccai G. Liquid-like water confined in stacks of biological membranes at 200 k and its relation to protein dynamics. Biophys J. 2005;89:3639-46 pubmed
    ..deuterium oxide (D(2)O) was obtained in the intermembrane spaces of a stack of purple membranes from Halobacterium salinarum by flash cooling to 77 K...
  49. Sudo Y, Furutani Y, Spudich J, Kandori H. Early photocycle structural changes in a bacteriorhodopsin mutant engineered to transmit photosensory signals. J Biol Chem. 2007;282:15550-8 pubmed
    ..We also succeeded in measurements of L minus initial state spectra of BR-T, which are the first FTIR spectra of L intermediates among sensory rhodopsins...
  50. Sasaki J, Nara T, Spudich E, Spudich J. Constitutive activity in chimeras and deletions localize sensory rhodopsin II/HtrII signal relay to the membrane-inserted domain. Mol Microbiol. 2007;66:1321-30 pubmed
    b>Halobacterium salinarum sensory rhodopsin II (HsSRII) is a phototaxis receptor for blue-light avoidance that relays signals to its tightly bound transducer HsHtrII (H. salinarum haloarchaeal transducer for SRII)...
  51. Teufel K, Bleiholder A, Griesbach T, Pfeifer F. Variations in the multiple tbp genes in different Halobacterium salinarum strains and their expression during growth. Arch Microbiol. 2008;190:309-18 pubmed publisher
    ..encoding TATA-box binding proteins (TBPs) was investigated in various strains and mutants of the archaeon Halobacterium salinarum. Six genes, tbpA through tbpF, are present in the genome of Hbt...
  52. Dunn R, McCoy J, Simsek M, Majumdar A, Chang S, RajBhandary U, et al. The bacteriorhodopsin gene. Proc Natl Acad Sci U S A. 1981;78:6744-8 pubmed
    ..Immediately downstream from this structure there is a sequence complementary to the 3' terminus of H. halobium 16S rRNA...
  53. Perálvarez Marín A, Marquez M, Bourdelande J, Querol E, Padrós E. Thr-90 plays a vital role in the structure and function of bacteriorhodopsin. J Biol Chem. 2004;279:16403-9 pubmed
    ..The interactions established by Thr-90 emerge as a general feature of archaeal rhodopsin proteins...
  54. Reindel S, Schmidt C, Anem ller S, Matzanke B. Expression and regulation pattern of ferritin-like DpsA in the archaeon Halobacterium Salinarum. Biometals. 2005;18:387-97 pubmed publisher
    Very recently, an iron-rich protein, DpsA, was isolated from the extreme halophilic euryarchaeon Halobacterium salinarum JW5 and characterized...
  55. Bandyopadhyay A, Krishnamoorthy G, Padhy L, Sonawat H. Kinetics of salt-dependent unfolding of [2Fe-2S] ferredoxin of Halobacterium salinarum. Extremophiles. 2007;11:615-25 pubmed
    The [2Fe-2S] ferredoxin from the extreme haloarchaeon Halobacterium salinarum is stable in high (>1.5 M) salt concentration. At low salt concentration the protein exhibits partial unfolding...
  56. Lazarova T, Querol E, Padrós E. Coupling between the retinal thermal isomerization and the Glu194 residue of bacteriorhodopsin. Photochem Photobiol. 2009;85:617-23 pubmed publisher
    ..After 14 days in the dark the 13-cis to all-trans ratio was 1:3 in the mutant, compared to 2:1 in the wild type. These data clearly indicate the involvement of Glu194 in control of the rate of all-trans to 13-cis thermal isomerization...
  57. Schmid A, Pan M, Sharma K, Baliga N. Two transcription factors are necessary for iron homeostasis in a salt-dwelling archaeon. Nucleic Acids Res. 2011;39:2519-33 pubmed publisher
    ..Under conditions of limited iron, the extremophile Halobacterium salinarum, a salt-loving archaeon, mounts a specific response to scavenge iron for growth...
  58. Plösser P, Pfeifer F. A bZIP protein from halophilic archaea: structural features and dimer formation of cGvpE from Halobacterium salinarum. Mol Microbiol. 2002;45:511-20 pubmed
    The cGvpE protein of Halobacterium salinarum PHH4 has been identified as transcriptional activator for the promoter of the c-gvpA gene encoding the major gas vesicle structural protein cGvpA...
  59. Herrmann U, Soppa J. Cell cycle-dependent expression of an essential SMC-like protein and dynamic chromosome localization in the archaeon Halobacterium salinarum. Mol Microbiol. 2002;46:395-409 pubmed
    The genome of Halobacterium salinarum encodes four proteins of the structural maintenance of chromosomes (SMC) protein superfamily. Two proteins form a novel subfamily and are named 'SMC-like proteins of H. salinarum' (Sph1 and Sph2)...
  60. Kish A, Kirkali G, Robinson C, Rosenblatt R, Jaruga P, Dizdaroglu M, et al. Salt shield: intracellular salts provide cellular protection against ionizing radiation in the halophilic archaeon, Halobacterium salinarum NRC-1. Environ Microbiol. 2009;11:1066-78 pubmed
    The halophilic archaeon Halobacterium salinarum NRC-1 was used as a model system to investigate cellular damage induced by exposure to high doses of ionizing radiation (IR)...
  61. Busch C, DiRuggiero J. MutS and MutL are dispensable for maintenance of the genomic mutation rate in the halophilic archaeon Halobacterium salinarum NRC-1. PLoS ONE. 2010;5:e9045 pubmed publisher
    The genome of the halophilic archaeon Halobacterium salinarum NRC-1 encodes for homologs of MutS and MutL, which are key proteins of a DNA mismatch repair pathway conserved in Bacteria and Eukarya...
  62. Bare J, Koide T, Reiss D, Tenenbaum D, Baliga N. Integration and visualization of systems biology data in context of the genome. BMC Bioinformatics. 2010;11:382 pubmed publisher
    ..Visualization and exploration of this data is critical to understanding the regulatory logic encoded in the genome by which the cell dynamically affects its physiology and interacts with its environment...
  63. Hofacker A, Schmitz K, Cichonczyk A, Sartorius Neef S, Pfeifer F. GvpE- and GvpD-mediated transcription regulation of the p-gvp genes encoding gas vesicles in Halobacterium salinarum. Microbiology. 2004;150:1829-38 pubmed
    The transcription of the 14 p-gvp genes involved in gas vesicle formation of Halobacterium salinarum PHH1 is driven by the four promoters pA, pD, pF and pO...
  64. Park S, Joo W, Choi J, Lee S, Kim C. Identification and characterization of inosine monophosphate dehydrogenase from Halobacterium salinarum. Proteomics. 2004;4:3632-41 pubmed
    Extremely halophilic Archaea, Halobacterium salinarum live in hypersaline habitats and maintain an osmotic balance of their cytoplasm by accumulating high concentrations of salt (mainly KCl)...
  65. Besir H, Zeth K, Bracher A, Heider U, Ishibashi M, Tokunaga M, et al. Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum. FEBS Lett. 2005;579:6595-600 pubmed
    Nucleoside diphosphate kinase from the halophilic archaeon Halobacterium salinarum was crystallized in a free state and a substrate-bound form with CDP. The structures were solved to a resolution of 2.35 and 2.2A, respectively...
  66. Srinivasan G, Krebs M, RajBhandary U. Translation initiation with GUC codon in the archaeon Halobacterium salinarum: implications for translation of leaderless mRNA and strict correlation between translation initiation and presence of mRNA. Mol Microbiol. 2006;59:1013-24 pubmed
    ..b>Halobacterium salinarum was used as the model organism and the bacterio-opsin gene (bop), which encodes the precursor of the ..
  67. Aivaliotis M, Gevaert K, Falb M, Tebbe A, Konstantinidis K, Bisle B, et al. Large-scale identification of N-terminal peptides in the halophilic archaea Halobacterium salinarum and Natronomonas pharaonis. J Proteome Res. 2007;6:2195-204 pubmed
    ..large-scale identification of N-terminal peptides from prokaryotes, of the two halophilic euryarchaeota Halobacterium salinarum and Natronomonas pharaonis...
  68. del Rosario R, Staudinger W, Streif S, Pfeiffer F, Mendoza E, Oesterhelt D. Modelling the CheY(D10K,Yl00W) Halobacterium salinarum mutant: sensitivity analysis allows choice of parameter to be modified in the phototaxis model. IET Syst Biol. 2007;1:207-21 pubmed
    A recent phototaxis model of Halobacterium salinarum composed of the signalling pathway and the switch complex of the motor explained all considered experimental data on spontaneous switching and response time to repellent or attractant ..
  69. Leuko S, Raftery M, Burns B, Walter M, Neilan B. Global protein-level responses of Halobacterium salinarum NRC-1 to prolonged changes in external sodium chloride concentrations. J Proteome Res. 2009;8:2218-25 pubmed publisher
    Responses to changes in external salinity were examined in Halobacterium salinarum NRC-1. H. salinarum NRC-1 grows optimally at 4.3 M NaCl and is capable of growth between 2.6 and 5.1 M NaCl...
  70. Chu L, Chen M, Setter J, Tsai Y, Yang H, Fang X, et al. New structural proteins of Halobacterium salinarum gas vesicle revealed by comparative proteomics analysis. J Proteome Res. 2011;10:1170-8 pubmed publisher
    The Halobacterium salinarum gas vesicle (GV) is an extremely stable intracellular organelle with air trapped inside a proteinaceous membrane...
  71. Shiu P, Ju Y, Chen H, Lee C. Facile isolation of purple membrane from Halobacterium salinarum via aqueous-two-phase system. Protein Expr Purif. 2013;89:219-24 pubmed publisher
    ..Generally, PM was purified from Halobacterium salinarum via a tedious and lengthy sucrose density gradient ultracentrifugation (SGU)...
  72. Peck R, Johnson E, Krebs M. Identification of a lycopene beta-cyclase required for bacteriorhodopsin biogenesis in the archaeon Halobacterium salinarum. J Bacteriol. 2002;184:2889-97 pubmed
    Biogenesis of the light-driven proton pump bacteriorhodopsin in the archaeon Halobacterium salinarum requires coordinate synthesis of the bacterioopsin apoprotein and carotenoid precursors of retinal, which serves as a covalently bound ..
  73. Ingoldsby L, Geoghegan K, Hayden B, Engel P. The discovery of four distinct glutamate dehydrogenase genes in a strain of Halobacterium salinarum. Gene. 2005;349:237-44 pubmed publisher
    ..work, two glutamate dehydrogenase (GDH) proteins were purified from a strain of the halophilic archaeon Halobacterium salinarum (NRC-36014). One of these, an NAD+-specific enzyme, was matched to a cloned gene from H...
  74. Sudo Y, Furutani Y, Kandori H, Spudich J. Functional importance of the interhelical hydrogen bond between Thr204 and Tyr174 of sensory rhodopsin II and its alteration during the signaling process. J Biol Chem. 2006;281:34239-45 pubmed
    ..The results argue for a critical process in signal relay occurring at this membrane interfacial region of the complex...
  75. Twellmeyer J, Wende A, Wolfertz J, Pfeiffer F, Panhuysen M, Zaigler A, et al. Microarray analysis in the archaeon Halobacterium salinarum strain R1. PLoS ONE. 2007;2:e1064 pubmed
    Phototrophy of the extremely halophilic archaeon Halobacterium salinarum was explored for decades. The research was mainly focused on the expression of bacteriorhodopsin and its functional properties...
  76. Strahl H, Greie J. The extremely halophilic archaeon Halobacterium salinarum R1 responds to potassium limitation by expression of the K+-transporting KdpFABC P-type ATPase and by a decrease in intracellular K+. Extremophiles. 2008;12:741-52 pubmed publisher
  77. Schmid A, Reiss D, Pan M, Koide T, Baliga N. A single transcription factor regulates evolutionarily diverse but functionally linked metabolic pathways in response to nutrient availability. Mol Syst Biol. 2009;5:282 pubmed publisher
    ..Here, we demonstrate that a single TrmB family transcription factor in Halobacterium salinarum NRC-1 globally coordinates functionally linked enzymes of diverse phylogeny in response to changes in ..
  78. Kaur A, Van P, Busch C, Robinson C, Pan M, Pang W, et al. Coordination of frontline defense mechanisms under severe oxidative stress. Mol Syst Biol. 2010;6:393 pubmed publisher
    ..We have constructed a systems model of OS response (OSR) for Halobacterium salinarum NRC-1 in an attempt to understand the architecture of its regulatory network that coordinates this ..
  79. Dummer A, Bonsall J, Cihla J, Lawry S, Johnson G, Peck R. Bacterioopsin-mediated regulation of bacterioruberin biosynthesis in Halobacterium salinarum. J Bacteriol. 2011;193:5658-67 pubmed publisher
    ..coordination, we study bacteriorhodopsin (BR), a light-induced proton pump in the halophilic archaeon Halobacterium salinarum. This complex consists of a retinal cofactor and bacterioopsin (BO), the BR apoprotein...
  80. Reindel S, Anem ller S, Sawaryn A, Matzanke B. The DpsA-homologue of the archaeon Halobacterium salinarum is a ferritin. Biochim Biophys Acta. 2002;1598:140-6 pubmed
    An iron-rich protein, DpsA(Hsal), was isolated from the archaeon Halobacterium salinarum sharing a sequence identity of 35% with the starvation-induced DNA-binding protein, DpsA, of Synechecoccus sp. PCC7942...
  81. McCready S, Marcello L. Repair of UV damage in Halobacterium salinarum. Biochem Soc Trans. 2003;31:694-8 pubmed
    ..However, there are also homologues of eukaryotic nucleotide-excision-repair genes ( Saccharomyces cerevisiae RAD3, RAD25 and RAD2 ) so there may be multiple repair mechanisms for UV damage in Halobacterium...
  82. Misquitta Y, Cherezov V, Havas F, Patterson S, Mohan J, Wells A, et al. Rational design of lipid for membrane protein crystallization. J Struct Biol. 2004;148:169-75 pubmed
    ..The same approach as used in this study should find application in extending the range of membrane proteins crystallizable by the in meso method and in tailoring transport of cubic phases for controlled delivery and uptake...
  83. Scheuch S, Pfeifer F. GvpD-induced breakdown of the transcriptional activator GvpE of halophilic archaea requires a functional p-loop and an arginine-rich region of GvpD. Microbiology. 2007;153:947-58 pubmed
    ..It was also found that the respective proteins cGvpE and cGvpD of Halobacterium salinarum and the heterologous pairs mcGvpD-cGvpE and cGvpD-mcGvpE were able to interact...
  84. Gonzalez O, Gronau S, Falb M, Pfeiffer F, Mendoza E, Zimmer R, et al. Reconstruction, modeling & analysis of Halobacterium salinarum R-1 metabolism. Mol Biosyst. 2008;4:148-59 pubmed publisher
    We present a genome-scale metabolic reconstruction for the extreme halophile Halobacterium salinarum. The reconstruction represents a summary of the knowledge regarding the organism's metabolism, and has already led to new research ..
  85. Pfeiffer F, Schuster S, Broicher A, Falb M, Palm P, Rodewald K, et al. Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1. Genomics. 2008;91:335-46 pubmed publisher
    We report the sequence of the Halobacterium salinarum strain R1 chromosome and its four megaplasmids. Our set of protein-coding genes is supported by extensive proteomic and sequence homology data...
  86. Schwaiger R, Schwarz C, Furtwängler K, Tarasov V, Wende A, Oesterhelt D. Transcriptional control by two leucine-responsive regulatory proteins in Halobacterium salinarum R1. BMC Mol Biol. 2010;11:40 pubmed publisher
    Archaea combine bacterial-as well as eukaryotic-like features to regulate cellular processes. Halobacterium salinarum R1 encodes eight leucine-responsive regulatory protein (Lrp)-homologues...
  87. Tóth Boconádi R, Keszthelyi L, Stoeckenius W. Photoexcitation of the O-intermediate in bacteriorhodopsin mutant L93A. Biophys J. 2003;84:3857-63 pubmed
    ..Both the OI- and OII-decays are apparently strongly inhibited in the mutant...
  88. Klein C, Garcia Rizo C, Bisle B, Scheffer B, Zischka H, Pfeiffer F, et al. The membrane proteome of Halobacterium salinarum. Proteomics. 2005;5:180-97 pubmed
    The identification of 114 integral membrane proteins from Halobacterium salinarum was achieved using liquid chromatography/tandem mass spectrometric (LC/MS/MS) techniques, representing 20% of the predicted alpha-helical transmembrane ..
  89. Trachtenberg S, Galkin V, Egelman E. Refining the structure of the Halobacterium salinarum flagellar filament using the iterative helical real space reconstruction method: insights into polymorphism. J Mol Biol. 2005;346:665-76 pubmed
    ..Here, we extend our previous study on the flagellar coiled filament structure of strain R1M1 of Halobacterium salinarum. We use strain M175 of H...
  90. Bonneau R, Facciotti M, Reiss D, Schmid A, Pan M, Kaur A, et al. A predictive model for transcriptional control of physiology in a free living cell. Cell. 2007;131:1354-65 pubmed
    ..We have constructed a model for this process in Halobacterium salinarum NRC-1 through the data-driven discovery of regulatory and functional interrelationships among ..
  91. Gonzalez O, Gronau S, Pfeiffer F, Mendoza E, Zimmer R, Oesterhelt D. Systems analysis of bioenergetics and growth of the extreme halophile Halobacterium salinarum. PLoS Comput Biol. 2009;5:e1000332 pubmed publisher
    b>Halobacterium salinarum is a bioenergetically flexible, halophilic microorganism that can generate energy by respiration, photosynthesis, and the fermentation of arginine...
  92. Whitfield D, Yu S, Dicaire C, Sprott G. Development of new glycosylation methodologies for the synthesis of archaeal-derived glycolipid adjuvants. Carbohydr Res. 2010;345:214-29 pubmed publisher
    ..describe a semi-synthetic approach where the lipid acceptor is derived from the biomass of the archaeon Halobacterium salinarum, and the glycosyl donors are chemically synthesized...