haloarcula

Summary

Summary: A genus of HALOBACTERIACEAE distinguished from other genera in the family by the presence of specific derivatives of TGD-2 polar lipids. Haloarcula are found in neutral saline environments such as salt lakes, marine salterns, and saline soils.

Top Publications

  1. Hochuli M, Patzelt H, Oesterhelt D, Wuthrich K, Szyperski T. Amino acid biosynthesis in the halophilic archaeon Haloarcula hispanica. J Bacteriol. 1999;181:3226-37 pubmed
    Biosynthesis of proteinogenic amino acids in the extremely halophilic archaeon Haloarcula hispanica was explored by using biosynthetically directed fractional 13C labeling with a mixture of 90% unlabeled and 10% uniformly 13C-labeled ..
  2. Cui H, Zhou P, Oren A, Liu S. Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium. Extremophiles. 2009;13:31-7 pubmed publisher
    All members of the genera Haloarcula and Halomicrobium whose names have been validly published were surveyed for 16S rRNA gene polymorphism, and the transcription of the genes from two species was investigated during growth at different ..
  3. Bath C, Dyall Smith M. His1, an archaeal virus of the Fuselloviridae family that infects Haloarcula hispanica. J Virol. 1998;72:9392-5 pubmed
    ..It was lytic, grew only on Haloarcula hispanica (titers of up to 10(11) PFU/ml), and displayed a lemon-shaped morphology (74 by 44 nm) previously ..
  4. Taran M. Utilization of petrochemical wastewater for the production of poly(3-hydroxybutyrate) by Haloarcula sp. IRU1. J Hazard Mater. 2011;188:26-8 pubmed publisher
    ..The ability of Haloarcula sp...
  5. Onodera M, Yatsunami R, Tsukimura W, Fukui T, Nakasone K, Takashina T, et al. Gene analysis, expression, and characterization of an intracellular ?-amylase from the extremely halophilic archaeon Haloarcula japonica. Biosci Biotechnol Biochem. 2013;77:281-8 pubmed
    b>Haloarcula japonica is an extremely halophilic archaeon that requires high concentrations of NaCl to grow. Recently the draft genome sequence of Ha. japonica was determined, and a gene encoding an ?-amylase, malA, was identified...
  6. Bamford D, Ravantti J, Rönnholm G, Laurinavicius S, Kukkaro P, Dyall Smith M, et al. Constituents of SH1, a novel lipid-containing virus infecting the halophilic euryarchaeon Haloarcula hispanica. J Virol. 2005;79:9097-107 pubmed
    ..In this investigation we rigorously show that SH1, an icosahedral dsDNA virus infecting Haloarcula hispanica, possesses lipid structural components that are selectively acquired from the host pool...
  7. Ozawa K, Harashina T, Yatsunami R, Nakamura S. Gene cloning, expression and partial characterization of cell division protein FtsZ1 from extremely halophilic archaeon Haloarcula japonica strain TR-1. Extremophiles. 2005;9:281-8 pubmed publisher
    The gene encoding a cell division protein FtsZ1 was cloned from an extremely halophilic archaeon, Haloarcula japonica strain TR-1...
  8. Dennis P, Ziesche S, Mylvaganam S. Transcription analysis of two disparate rRNA operons in the halophilic archaeon Haloarcula marismortui. J Bacteriol. 1998;180:4804-13 pubmed
    The genome of the halophilic archaeon Haloarcula marismortui contains two rRNA operons designated rrnA and rrnB...
  9. Han J, Lu Q, Zhou L, Liu H, Xiang H. Identification of the polyhydroxyalkanoate (PHA)-specific acetoacetyl coenzyme A reductase among multiple FabG paralogs in Haloarcula hispanica and reconstruction of the PHA biosynthetic pathway in Haloferax volcanii. Appl Environ Microbiol. 2009;75:6168-75 pubmed publisher
    ..paralogs, encodes the polyhydroxyalkanoate (PHA)-specific acetoacetyl coenzyme A (acetoacetyl-CoA) reductase in Haloarcula hispanica...

More Information

Publications91

  1. Taran M, Amirkhani H. Strategies of poly(3-hydroxybutyrate) synthesis by Haloarcula sp. IRU1 utilizing glucose as carbon source: Optimization of culture conditions by Taguchi methodology. Int J Biol Macromol. 2010;47:632-4 pubmed publisher
    We investigated optimization of poly(3-hydroxybutyrate) (PHB) production by Haloarcula sp. IRU1 in a batch culture system...
  2. Porter K, Russ B, Yang J, Dyall Smith M. The transcription programme of the protein-primed halovirus SH1. Microbiology. 2008;154:3599-608 pubmed publisher
    ..The virus lytically infects the haloarchaeon Haloarcula hispanica, and carries a 30...
  3. Hutcheon G, Vasisht N, Bolhuis A. Characterisation of a highly stable alpha-amylase from the halophilic archaeon Haloarcula hispanica. Extremophiles. 2005;9:487-95 pubmed
    ..Here, we present the characterisation of a secreted alpha-amylase (AmyH) from the halophilic archaeon Haloarcula hispanica...
  4. Fukushima T, Mizuki T, Echigo A, Inoue A, Usami R. Organic solvent tolerance of halophilic alpha-amylase from a Haloarchaeon, Haloarcula sp. strain S-1. Extremophiles. 2005;9:85-9 pubmed
    A halophilic archaeon, Haloarcula sp. strain S-1, produced extracellular organic solvent-tolerant alpha-amylase. Molecular mass of the enzyme was estimated to be 70 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis...
  5. Liu H, Han J, Liu X, Zhou J, Xiang H. Development of pyrF-based gene knockout systems for genome-wide manipulation of the archaea Haloferax mediterranei and Haloarcula hispanica. J Genet Genomics. 2011;38:261-9 pubmed publisher
    The haloarchaea Haloferax mediterranei and Haloarcula hispanica are both polyhydroxyalkanoate producers in the domain Archaea, and they are becoming increasingly attractive for research and biotechnology due to their unique genetic and ..
  6. Li M, Wang R, Zhao D, Xiang H. Adaptation of the Haloarcula hispanica CRISPR-Cas system to a purified virus strictly requires a priming process. Nucleic Acids Res. 2014;42:2483-92 pubmed publisher
    ..Infected by a newly isolated virus HHPV-2, Haloarcula hispanica type I-B CRISPR system acquired spacers discriminatively from viral sequences...
  7. Oren A, Ventosa A, Gutierrez M, Kamekura M. Haloarcula quadrata sp. nov., a square, motile archaeon isolated from a brine pool in Sinai (Egypt). Int J Syst Bacteriol. 1999;49 Pt 3:1149-55 pubmed
    ..1 mol%) and its growth characteristics, the isolate could be assigned to the genus Haloarcula. However, phylogenetic analysis of the two 16S rRNA genes detected in this organism and low DNA-DNA ..
  8. Woods W, Ngui K, Dyall Smith M. An improved transposon for the halophilic archaeon Haloarcula hispanica. J Bacteriol. 1999;181:7140-2 pubmed
    An improved transposon (ThD73) for Haloarcula hispanica is described. Based on the halobacterial insertion sequence ISH28, it showed little target sequence specificity but was biased toward a lower G+C content...
  9. Yagasaki J, Suzuki D, Ihara K, Inoue K, Kikukawa T, Sakai M, et al. Spectroscopic studies of a sensory rhodopsin I homologue from the archaeon Haloarcula vallismortis. Biochemistry. 2010;49:1183-90 pubmed publisher
    ..In this study, we expressed a SRI homologue from the archaeon Haloarcula vallismortis (HvSRI) as a recombinant protein which uses all-trans-retinal as a chromophore...
  10. Dyall Smith M, Tang S, Bath C. Haloarchaeal viruses: how diverse are they?. Res Microbiol. 2003;154:309-13 pubmed
    ..Here we provide an outline of the reported haloviruses, including fusiform and unpublished isolates from this laboratory, and we discuss their diversity and the future directions for this research...
  11. Mizuki T, Kamekura M, Dassarma S, Fukushima T, Usami R, Yoshida Y, et al. Ureases of extreme halophiles of the genus Haloarcula with a unique structure of gene cluster. Biosci Biotechnol Biochem. 2004;68:397-406 pubmed
    ..Only 4 strains of the genus Haloarcula, Har. aidinensis, Har. hispanica, Har. japonica, and Har. marismortui were finally shown as the urease producers...
  12. Li M, Wang R, Xiang H. Haloarcula hispanica CRISPR authenticates PAM of a target sequence to prime discriminative adaptation. Nucleic Acids Res. 2014;42:7226-35 pubmed publisher
    ..The adaptation mechanism avoiding acquiring 'self' DNA fragments is poorly understood. In Haloarcula hispanica, we previously showed that CRISPR adaptation requires being primed by a pre-existing spacer partially ..
  13. Liu H, Wu Z, Li M, Zhang F, Zheng H, Han J, et al. Complete genome sequence of Haloarcula hispanica, a Model Haloarchaeon for studying genetics, metabolism, and virus-host interaction. J Bacteriol. 2011;193:6086-7 pubmed publisher
    b>Haloarcula hispanica is an extremely halophilic archaeon that has an unusually low restriction barrier and is therefore significant for studying archaeal genetics, metabolism, and virus-host interactions...
  14. Sun C, Zhou M, Li Y, Xiang H. Molecular characterization of the minimal replicon and the unidirectional theta replication of pSCM201 in extremely halophilic archaea. J Bacteriol. 2006;188:8136-44 pubmed publisher
    A 3,463-bp plasmid, pSCM201, was isolated from a halophilic archaeon, Haloarcula sp. strain AS7094...
  15. Wendoloski D, Ferrer C, Dyall Smith M. A new simvastatin (mevinolin)-resistance marker from Haloarcula hispanica and a new Haloferax volcanii strain cured of plasmid pHV2. Microbiology. 2001;147:959-64 pubmed publisher
    ..chromosome of Haloferax, a homologue of the hmg determinant was obtained from the distantly related organism, Haloarcula hispanica...
  16. Kivelä H, Roine E, Kukkaro P, Laurinavicius S, Somerharju P, Bamford D. Quantitative dissociation of archaeal virus SH1 reveals distinct capsid proteins and a lipid core. Virology. 2006;356:4-11 pubmed
    ..between the capsid and the membrane of icosahedral SH1 virus, an archaeal virus infecting extreme halophilic Haloarcula hispanica cells...
  17. Roine E, Kukkaro P, Paulin L, Laurinavicius S, Domanska A, Somerharju P, et al. New, closely related haloarchaeal viral elements with different nucleic Acid types. J Virol. 2010;84:3682-9 pubmed publisher
    ..the search for haloarchaeal viruses, we isolated and characterized a new pleomorphic lipid-containing virus, Haloarcula hispanica pleomorphic virus 1 (HHPV-1), that infects the halophilic archaeon Haloarcula hispanica...
  18. Kwan D, Thomas J, Bolhuis A. Bioenergetic requirements of a Tat-dependent substrate in the halophilic archaeon Haloarcula hispanica. FEBS J. 2008;275:6159-67 pubmed publisher
    ..In this study, we have analysed the translocation of a secretory protein (AmyH) from the haloarchaeon Haloarcula hispanica...
  19. Yatsunami R, Kawakami T, Ohtani H, Nakamura S. A novel bacteriorhodopsin-like protein from Haloarcula japonica strain TR-1: gene cloning, sequencing, and transcript analysis. Extremophiles. 2000;4:109-14 pubmed
    The gene encoding a novel bacteriorhodopsin (bR)-like protein from Haloarcula japonica strain TR-1 was cloned and sequenced...
  20. Jaakkola S, Penttinen R, Vilén S, Jalasvuori M, Rönnholm G, Bamford J, et al. Closely related archaeal Haloarcula hispanica icosahedral viruses HHIV-2 and SH1 have nonhomologous genes encoding host recognition functions. J Virol. 2012;86:4734-42 pubmed publisher
    ..Here, we studied the host range, life cycle, biochemical composition, and genomic sequence of a new isolate, Haloarcula hispanica icosahedral virus 2 (HHIV-2), which resembles SH1 despite being isolated from a different location...
  21. Gutierrez M, Kamekura M, Holmes M, Dyall Smith M, Ventosa A. Taxonomic characterization of Haloferax sp. (" H. alicantei") strain Aa 2.2: description of Haloferax lucentensis sp. nov. Extremophiles. 2002;6:479-83 pubmed
    ..2 as a new species, Haloferax lucentensis sp. nov. The type strain is Aa 2.2 (=JCM 9276=NCIMB 13854=CIP 107410=DSM 14919=CECT 5871=CCM 7023)...
  22. Porter K, Kukkaro P, Bamford J, Bath C, Kivelä H, Dyall Smith M, et al. SH1: A novel, spherical halovirus isolated from an Australian hypersaline lake. Virology. 2005;335:22-33 pubmed
    ..Isolated from a hypersaline lake, SH1 is divalent, producing clear plaques on Haloarcula hispanica and a natural Halorubrum isolate...
  23. Wakai H, Nakamura S, Kawasaki H, Takada K, Mizutani S, Aono R, et al. Cloning and sequencing of the gene encoding the cell surface glycoprotein of Haloarcula japonica strain TR-1. Extremophiles. 1997;1:29-35 pubmed
    The triangular disk-shaped halophilic archaeon Haloarcula japonica strain TR-1 has a glycoprotein on its cell surface. The complete gene encoding the cell surface glycoprotein (CSG) was cloned and sequenced...
  24. Sugimori D, Ichimata T, Ikeda A, Nakamura S. Purification and characterization of a ferredoxin from Haloarcula japonica strain TR-1. Biometals. 2000;13:23-8 pubmed
    A ferredoxin (Fd) was purified from the extremely halophilic archaeon, Haloarcula japonica strain TR-1, to electrophoretic homogeneity...
  25. Gupta R, Naushad S, Fabros R, Adeolu M. A phylogenomic reappraisal of family-level divisions within the class Halobacteria: proposal to divide the order Halobacteriales into the families Halobacteriaceae, Haloarculaceae fam. nov., and Halococcaceae fam. nov., and the order Haloferacales in. Antonie Van Leeuwenhoek. 2016;109:565-87 pubmed publisher
    ..We also present 40 CSIs and 234 CSPs which are characteristic of Haloarcula, Halococcus, Haloferax, or Halorubrum...
  26. Miyashita Y, Ohmae E, Ikura T, Nakasone K, Katayanagi K. Halophilic mechanism of the enzymatic function of a moderately halophilic dihydrofolate reductase from Haloarcula japonica strain TR-1. Extremophiles. 2017;21:591-602 pubmed publisher
    Dihydrofolate (DHF) reductase coded by a plasmid of the extremely halophilic archaeon Haloarcula japonica strain TR-1 (HjDHFR P1) shows moderate halophilicity on enzymatic activity at pH 6...
  27. Salgaonkar B, Braganca J. Utilization of Sugarcane Bagasse by Halogeometricum borinquense Strain E3 for Biosynthesis of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate). Bioengineering (Basel). 2017;4: pubmed publisher
    ..strain E3 showed better growth and PHA accumulation as compared to Haloferaxvolcanii strain BBK2, Haloarcula japonica strain BS2, and Halococcus salifodinae strain BK6...
  28. Martin Del Campo M, Camacho R, Mateos Díaz J, Müller Santos M, Córdova J, Rodríguez J. Solid-state fermentation as a potential technique for esterase/lipase production by halophilic archaea. Extremophiles. 2015;19:1121-32 pubmed publisher
    ..Three halophilic archaea: Natronococcus sp. TC6, Halobacterium sp. NRC-1 and Haloarcula marismortui were cultured by submerged fermentation (SmF) and by SSF; an improvement of 1.3- to 6...
  29. Gil Cartón D, Jaakkola S, Charro D, Peralta B, Castaño Díez D, Oksanen H, et al. Insight into the Assembly of Viruses with Vertical Single β-barrel Major Capsid Proteins. Structure. 2015;23:1866-1877 pubmed publisher
    ..microscopy techniques, we solved the structure of euryarchaeal, halophilic, internal membrane-containing Haloarcula hispanica icosahedral virus 2 (HHIV-2)...
  30. Jang E, Shim H, Ryu B, An D, Yoo W, Kim K, et al. Preparation of cobalt nanoparticles from polymorphic bacterial templates: A novel platform for biocatalysis. Int J Biol Macromol. 2015;81:747-53 pubmed publisher
    ..and Haloarcula vallismortis as bacterial templates...
  31. Quadri I, Hassani I, L Haridon S, Chalopin M, Hacene H, Jebbar M. Characterization and antimicrobial potential of extremely halophilic archaea isolated from hypersaline environments of the Algerian Sahara. Microbiol Res. 2016;186-187:119-31 pubmed publisher
    ..of 10 archaeal genera within the class Halobacteria: Natrinema (13 strains), Natrialba (12 strains), Haloarcula (4 strains), Halopiger (4 strains), Haloterrigena (3 strains), Halorubrum (2 strains), Halostagnicola (2 strains)..
  32. Hung C, Chen X, Ko Y, Kobayashi T, Yang C, Yabushita A. Schiff Base Proton Acceptor Assists Photoisomerization of Retinal Chromophores in Bacteriorhodopsin. Biophys J. 2017;112:2503-2519 pubmed publisher
    ..study, we investigated the ultrafast dynamics of bacteriorhodopsins (BRs) from Haloquadratum walsbyi (HwBR) and Haloarcula marismortui (HmBRI and HmBRII)...
  33. Binnewies T, Hallin P, Staerfeldt H, Ussery D. Genome Update: proteome comparisons. Microbiology. 2005;151:1-4 pubmed
  34. Namwong S, Tanasupawat S, Kudo T, Itoh T. Haloarcula salaria sp. nov. and Haloarcula tradensis sp. nov., isolated from salt in Thai fish sauce. Int J Syst Evol Microbiol. 2011;61:231-6 pubmed publisher
    ..sequences revealed that strains HST01-2R(T) and HST03(T) were placed in the radiation of species of the genus Haloarcula. The chemotaxonomic properties of the two strains, i.e...
  35. Nercessian D, Di Meglio L, De Castro R, Paggi R. Exploring the multiple biotechnological potential of halophilic microorganisms isolated from two Argentinean salterns. Extremophiles. 2015;19:1133-43 pubmed publisher
    ..While all the isolates exhibited at least one of the tested activities or biocompounds, the species belonging to Haloarcula genus were the most active, also producing antimicrobial compounds against their counterparts...
  36. Wakai S, Abe A, Fujii S, Nakasone K, Sambongi Y. Pyrophosphate hydrolysis in the extremely halophilic archaeon Haloarcula japonica is catalyzed by a single enzyme with a broad ionic strength range. Extremophiles. 2017;21:471-477 pubmed publisher
    The soluble protein fraction of the extremely halophilic archaeon Haloarcula japonica exhibits substantial inorganic pyrophosphate (PPi) hydrolysis activity in the presence of 2-4 M NaCl (Wakai et al, J Biol Chem 288:29247-29251, 2013), ..
  37. Menasria T, Aguilera M, Hocine H, Benammar L, Ayachi A, Si Bachir A, et al. Diversity and bioprospecting of extremely halophilic archaea isolated from Algerian arid and semi-arid wetland ecosystems for halophilic-active hydrolytic enzymes. Microbiol Res. 2018;207:289-298 pubmed publisher
    ..16S rRNA gene sequencing, the isolates were assigned to seven phylotypes within the class Halobacteria, namely Haloarcula, Halococcus, Haloferax, Halogeometricum, Haloterrigena, Natrialba, and Natrinema...
  38. Mazguene S, Rossi M, Gogliettino M, Palmieri G, Cocca E, Mirino S, et al. Isolation and characterization from solar salterns of North Algeria of a haloarchaeon producing a new halocin. Extremophiles. 2018;22:259-270 pubmed publisher
    ..while the best indicator microorganism, showing high sensitivity toward halocin, was related to Haloarcula genus...
  39. Fairley D, Boyd D, Sharma N, Allen C, Morgan P, Larkin M. Aerobic metabolism of 4-hydroxybenzoic acid in Archaea via an unusual pathway involving an intramolecular migration (NIH shift). Appl Environ Microbiol. 2002;68:6246-55 pubmed
    A novel haloarchaeal strain, Haloarcula sp. strain D1, grew aerobically on 4-hydroxybenzoic acid (4HBA) as a sole carbon and energy source and is the first member of the domain Archaea reported to do so...
  40. Yang D, Li Q, Huang J, Chen M. [Diversity and enzyme-producing activity of culturable halophilic bacteria in Daishan Saltern of East China]. Ying Yong Sheng Tai Xue Bao. 2012;23:3103-8 pubmed
    ..The dominant halophilic strains were of Halomonas and Haloarcula, with 46.8% in halophilic bacteria and 49.1% in halophilic archaea group, respectively...
  41. Byron K, Laing C, Wen D, Wang J. A computational approach to finding RNA tertiary motifs in genomic sequences: a case study. Recent Pat DNA Gene Seq. 2013;7:115-22 pubmed
    ..Experimental results demonstrate the effectiveness of our approach. ..
  42. Santoni E, Jakopitsch C, Obinger C, Smulevich G. Manipulating the covalent link between distal side tryptophan, tyrosine, and methionine in catalase-peroxidases: an electronic absorption and resonance Raman study. Biopolymers. 2004;74:46-50 pubmed
    ..The results are compared with those previously reported for the Trp122Ala and Trp122Phe variants. ..
  43. Li X, Yu H. Halostable cellulase with organic solvent tolerance from Haloarcula sp. LLSG7 and its application in bioethanol fermentation using agricultural wastes. J Ind Microbiol Biotechnol. 2013;40:1357-65 pubmed publisher
    ..and physiological characterization along with 16S rRNA gene sequence analysis placed the isolate in the genus Haloarcula. Cellulase production was strongly influenced by the salinity of the culture medium with the maximum obtained in ..
  44. Demina T, Pietilä M, Svirskaitė J, Ravantti J, Atanasova N, Bamford D, et al. HCIV-1 and Other Tailless Icosahedral Internal Membrane-Containing Viruses of the Family Sphaerolipoviridae. Viruses. 2017;9: pubmed publisher
    ..A recently described tailless icosahedral haloarchaeal virus, Haloarcula californiae icosahedral virus 1 (HCIV-1), has a double-stranded DNA genome and an internal membrane lining ..
  45. Pietilä M, Atanasova N, Oksanen H, Bamford D. Modified coat protein forms the flexible spindle-shaped virion of haloarchaeal virus His1. Environ Microbiol. 2013;15:1674-86 pubmed publisher
    ..In the present work, we studied spindle-shaped virus His1 infecting an extremely halophilic euryarchaeon, Haloarcula hispanica. We demonstrate that His1 tolerates a variety of salinities, even lower than that of seawater...
  46. Pietilä M, Laurinmäki P, Russell D, Ko C, Jacobs Sera D, Hendrix R, et al. Structure of the archaeal head-tailed virus HSTV-1 completes the HK97 fold story. Proc Natl Acad Sci U S A. 2013;110:10604-9 pubmed publisher
    ..electron cryomicroscopy, and image reconstruction that the major coat protein fold of newly isolated archaeal Haloarcula sinaiiensis tailed virus 1 has the canonical coat protein fold of HK97...
  47. Demina T, Atanasova N, Pietilä M, Oksanen H, Bamford D. Vesicle-like virion of Haloarcula hispanica pleomorphic virus 3 preserves high infectivity in saturated salt. Virology. 2016;499:40-51 pubmed publisher
    ..Here we report a newly isolated archaeal halovirus, Haloarcula hispanica pleomorphic virus 3 (HHPV3)...
  48. Oren A. Pyruvate: A key Nutrient in Hypersaline Environments?. Microorganisms. 2015;3:407-16 pubmed publisher
    ..Some halophilic Archaea (Halorubrum saccharovorum, Haloarcula spp...
  49. Borjian F, Han J, Hou J, Xiang H, Zarzycki J, Berg I. Malate Synthase and β-Methylmalyl Coenzyme A Lyase Reactions in the Methylaspartate Cycle in Haloarcula hispanica. J Bacteriol. 2017;199: pubmed publisher
    ..the CitE-like enzyme superfamily, malyl-CoA lyase/thioesterase (haloarchaeal malate synthase [hMS]; Hah_2476 in Haloarcula hispanica) and β-methylmalyl-CoA lyase (haloarchaeal β-methylmalyl-CoA lyase [hMCL]; Hah_1341)...
  50. Lynch E, Langille M, Darling A, Wilbanks E, Haltiner C, Shao K, et al. Sequencing of seven haloarchaeal genomes reveals patterns of genomic flux. PLoS ONE. 2012;7:e41389 pubmed publisher
    We report the sequencing of seven genomes from two haloarchaeal genera, Haloferax and Haloarcula. Ease of cultivation and the existence of well-developed genetic and biochemical tools for several diverse haloarchaeal species make ..
  51. Szyperski T, Glaser R, Hochuli M, Fiaux J, Sauer U, Bailey J, et al. Bioreaction network topology and metabolic flux ratio analysis by biosynthetic fractional 13C labeling and two-dimensional NMR spectroscopy. Metab Eng. 1999;1:189-97 pubmed
    ..This novel approach, which recently also has been employed in conjunction with metabolic flux balancing to obtain reliable estimates of in vivo fluxes, enables efficient support of metabolic engineering and biotechnology process design. ..
  52. Cheng F, Gong L, Zhao D, Yang H, Zhou J, Li M, et al. Harnessing the native type I-B CRISPR-Cas for genome editing in a polyploid archaeon. J Genet Genomics. 2017;44:541-548 pubmed publisher
    ..we harnessed the native type I-B CRISPR-Cas system for precise genome editing in the polyploid haloarchaeon Haloarcula hispanica...
  53. Demina T, Pietilä M, Svirskaitė J, Ravantti J, Atanasova N, Bamford D, et al. Archaeal Haloarcula californiae Icosahedral Virus 1 Highlights Conserved Elements in Icosahedral Membrane-Containing DNA Viruses from Extreme Environments. MBio. 2016;7: pubmed publisher
    ..Here, we introduce Haloarcula californiae icosahedral virus 1 (HCIV-1), a halophilic euryarchaeal virus originating from salt crystals...
  54. Anton J, Llobet Brossa E, Rodriguez Valera F, Amann R. Fluorescence in situ hybridization analysis of the prokaryotic community inhabiting crystallizer ponds. Environ Microbiol. 1999;1:517-23 pubmed
    ..Additionally, FISH with a probe targeted to the genus Haloarcula, members of which are frequently isolated from this environment, indicated that this genus accounts for less ..
  55. Hansen J, Schmeing T, Moore P, Steitz T. Structural insights into peptide bond formation. Proc Natl Acad Sci U S A. 2002;99:11670-5 pubmed
    ..of both A and P site substrate and product analogues, as well as an intermediate analogue, bound to the Haloarcula marismortui 50S ribosomal subunit...
  56. Attar A, Ogan A, Yucel S, Turan K. The potential of archaeosomes as carriers of pDNA into mammalian cells. Artif Cells Nanomed Biotechnol. 2016;44:710-6 pubmed publisher
    ..The transfection profiles of efficient archaeosomes are proved to have a long shelf-life when maintained at room temperature. Thus, the archaeal lipids have the potential to be used as transfection reagents in vitro. ..
  57. Matsuo T, Ikeda A, Seki H, Ichimata T, Sugimori D, Nakamura S. Cloning and expression of the ferredoxin gene from extremely halophilic archaeon Haloarculajaponica strain TR-1. Biometals. 2001;14:135-42 pubmed
    ..japonica host cells. The absorption and ESR spectra of the Fd/His Tag fusion protein revealed the presence of a [2Fe-2S] cluster which is characteristic of native Ha. japonica Fd...
  58. Li M, Gong L, Zhao D, Zhou J, Xiang H. The spacer size of I-B CRISPR is modulated by the terminal sequence of the protospacer. Nucleic Acids Res. 2017;45:4642-4654 pubmed publisher
    ..Here, we explored the causes of this heterogeneity in Haloarcula hispanica I-B CRISPR...
  59. Mohan S, Hsiao C, VanDeusen H, Gallagher R, Krohn E, Kalahar B, et al. Mechanism of RNA double helix-propagation at atomic resolution. J Phys Chem B. 2009;113:2614-23 pubmed
    ..A base can be (i) unstacked and unpaired, (ii) stacked and paired, or (ii) stacked and unpaired (only on the 3' strand). The data suggests that helices of DNA and of RNA do not propagate by similar mechanisms...
  60. Vreeland R, Straight S, Krammes J, Dougherty K, Rosenzweig W, Kamekura M. Halosimplex carlsbadense gen. nov., sp. nov., a unique halophilic archaeon, with three 16S rRNA genes, that grows only in defined medium with glycerol and acetate or pyruvate. Extremophiles. 2002;6:445-52 pubmed
    ..Inferred phylogenies indicate that the organism belongs to a deep branch in the line of Haloarcula and Halorhabdus...
  61. Oren A. Bioenergetic aspects of halophilism. Microbiol Mol Biol Rev. 1999;63:334-48 pubmed
  62. Miao D, Sun C, Xiang H. [Construction and application of a novel shuttle expression vector based on haloarchaeal plasmid pSCM201]. Wei Sheng Wu Xue Bao. 2009;49:1040-7 pubmed
    ..For gene expression and function analysis of specific genes in model strain Haloarcula hispanica, a novel shuttle expression vector based on pSCM201 was developed.
  63. Park J, Vreeland R, Cho B, Lowenstein T, Timofeeff M, Rosenzweig W. Haloarchaeal diversity in 23, 121 and 419 MYA salts. Geobiology. 2009;7:515-23 pubmed publisher
    ..Only Haloarcula and Halorubrum DNA was found in 23 MYA salt. Older crystals contained unclassified groups and Halobacterium...
  64. Qvist J, Persson E, Mattea C, Halle B. Time scales of water dynamics at biological interfaces: peptides, proteins and cells. Faraday Discuss. 2009;141:131-44; discussion 175-207 pubmed
    ..There is no significant difference in cell water dynamics between mesophilic and halophilic organisms, despite the high K+ and Na+ concentrations in the latter...
  65. Taran M, Monazah A, Asadi N. Production of a novel biomacromolecule for nanodevices from glycerol as carbon source in different conditions. Int J Biol Macromol. 2011;49:955-7 pubmed publisher
    ..The production of cruxrhodopsin in optimal conditions was 139.86 mg/l. In conclusion, halophilic microorganism Haloarcula sp. IRU1 could be a potential microorganism for production of cruxrhodopsin from glycerol in different conditions...
  66. Mei S, Sun C, Liu X, Lu Q, Cai L, Li Y, et al. The helix-loop-helix motif at the N terminus of HalI is essential for its immunity function against halocin C8. J Bacteriol. 2008;190:6501-8 pubmed publisher
    ..These results demonstrated that HalI sequestered the activity of HalC8 through specific and direct binding...
  67. Ogan A, Danis O, Gozuacik A, Cakmar E, Birbir M. Production of cellulase by immobilized whole cells of Haloarcula. Prikl Biokhim Mikrobiol. 2012;48:490-3 pubmed
    ..The production of cellulase using free and immobilized cells of halophilic archaeal strain Haloarcula 2TK2 isolated from Tuzkoy Salt Mine and capable of producing cellulose was studied...
  68. Tapilatu Y, Grossi V, Acquaviva M, Militon C, Bertrand J, Cuny P. Isolation of hydrocarbon-degrading extremely halophilic archaea from an uncontaminated hypersaline pond (Camargue, France). Extremophiles. 2010;14:225-31 pubmed publisher
    ..Five alkane-degrading halophilic archaeal strains were isolated: one (strain MSNC 2) was closely related to Haloarcula and three (strains MSNC 4, MSNC 14, and MSNC 16) to Haloferax...
  69. Svirskaitė J, Oksanen H, Daugelavičius R, Bamford D. Monitoring Physiological Changes in Haloarchaeal Cell during Virus Release. Viruses. 2016;8:59 pubmed publisher
    ..To address this deficit, we utilized four viruses that infect Haloarcula hispanica that represent the four virion morphotypes currently known for halophilic euryarchaeal viruses: (1) ..
  70. Yang Y, Cui H, Zhou P, Liu S. Haloarcula amylolytica sp. nov., an extremely halophilic archaeon isolated from Aibi salt lake in Xin-Jiang, China. Int J Syst Evol Microbiol. 2007;57:103-6 pubmed
    ..4 mol%) and growth characteristics, the organism could be assigned to the genus Haloarcula. Phenotypic differences and low DNA-DNA hybridization values to related Haloarcula species distinguished strain ..
  71. Fairley D, Wang G, Rensing C, Pepper I, Larkin M. Expression of gentisate 1,2-dioxygenase (gdoA) genes involved in aromatic degradation in two haloarchaeal genera. Appl Microbiol Biotechnol. 2006;73:691-5 pubmed
    ..In Haloarcula sp...
  72. Porter K, Tang S, Chen C, Chiang P, Hong M, Dyall Smith M. PH1: an archaeovirus of Haloarcula hispanica related to SH1 and HHIV-2. Archaea. 2013;2013:456318 pubmed publisher
    Halovirus PH1 infects Haloarcula hispanica and was isolated from an Australian salt lake. The burst size in single-step growth conditions was 50-100 PFU/cell, but cell density did not decrease until well after the rise (4-6 hr p.i...
  73. Thirumoorthy K, Nandi N. Homochiral preference in peptide synthesis in ribosome: role of amino terminal, peptidyl terminal, and U2620. J Phys Chem B. 2007;111:9999-10004 pubmed
    ..studies based on a model of peptidyl transferase center from the crystal structure of the ribosomal part of Haloarcula marismortui...
  74. Yun J, Song H, Roh S, Jung M, Kim P, Bae J. Complete genome sequence of Haloarcula sp. CBA1115 isolated from non-purified solar salts. Mar Genomics. 2015;23:19-21 pubmed publisher
    b>Haloarcula sp. CBA1115, isolated from non-purified solar salts from South Korea, is a halophilic archaeon belonging to the family Halobacteriaceae. Here, we present the complete genome sequence of the strain Haloarcula sp...
  75. Yonath A. The search and its outcome: high-resolution structures of ribosomal particles from mesophilic, thermophilic, and halophilic bacteria at various functional states. Annu Rev Biophys Biomol Struct. 2002;31:257-73 pubmed
    ..In unbound subunits, the features creating intersubunit bridges within associated ribosomes lie on the interface surface, and the features that bind factors and substrates reach toward the binding site only when conditions are ripe...
  76. Zeng C, Zhao Y, Cui C, Zhang H, Zhu J, Tang X, et al. Characterization of the Haloarcula hispanica amyH gene promoter, an archaeal promoter that confers promoter activity in Escherichia coli. Gene. 2009;442:1-7 pubmed publisher
    ..In the present study, the promoter region of the halophilic archaeon Haloarcula hispanica's amyH gene was isolated and characterized, and it was surprisingly revealed that the amyH gene ..
  77. Hanhijärvi K, Ziedaite G, Pietilä M, Hæggstrom E, Bamford D. DNA ejection from an archaeal virus--a single-molecule approach. Biophys J. 2013;104:2264-72 pubmed publisher
    ..Here we describe a DNA release system that operates in an archaeal virus. This virus infects an archaeon Haloarcula hispanica that was isolated from a hypersaline environment...
  78. Liu H, Luo Y, Han J, Wu J, Wu Z, Feng D, et al. Proteome reference map of Haloarcula hispanica and comparative proteomic and transcriptomic analysis of polyhydroxyalkanoate biosynthesis under genetic and environmental perturbations. J Proteome Res. 2013;12:1300-15 pubmed publisher
    ..In this study, a combined proteomic and transcriptomic approach was employed in Haloarcula hispanica, a model haloarchaeon that accumulates poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) under ..
  79. Simonovic M, Steitz T. Peptidyl-CCA deacylation on the ribosome promoted by induced fit and the O3'-hydroxyl group of A76 of the unacylated A-site tRNA. RNA. 2008;14:2372-8 pubmed publisher
    ..Two new structures of the large ribosomal subunit of Haloarcula marismortui complexed with a peptidyl-tRNA analog in the P site and two oligonucleotide mimics of unacylated ..
  80. Yamamura A, Ichimura T, Kamekura M, Mizuki T, Usami R, Makino T, et al. Molecular mechanism of distinct salt-dependent enzyme activity of two halophilic nucleoside diphosphate kinases. Biophys J. 2009;96:4692-700 pubmed publisher
    Nucleoside diphosphate kinases from haloarchaea Haloarcula quadrata (NDK-q) and H. sinaiiensis (NDK-s) are identical except for one out of 154 residues, i.e., Arg(31) in NDK-q and Cys(31) in NDK-s...
  81. Hansen J, Moore P, Steitz T. Structures of five antibiotics bound at the peptidyl transferase center of the large ribosomal subunit. J Mol Biol. 2003;330:1061-75 pubmed
    ..chloramphenicol, sparsomycin, blasticidin S, and virginiamycin M bound to the large ribosomal subunit of Haloarcula marismortui have been determined at 3.0A resolution...
  82. Goo Y, Roach J, Glusman G, Baliga N, Deutsch K, Pan M, et al. Low-pass sequencing for microbial comparative genomics. BMC Genomics. 2004;5:3 pubmed
    We studied four extremely halophilic archaea by low-pass shotgun sequencing: (1) the metabolically versatile Haloarcula marismortui; (2) the non-pigmented Natrialba asiatica; (3) the psychrophile Halorubrum lacusprofundi and (4) the Dead ..