sulfolobus solfataricus

Summary

Summary: A species of thermoacidophilic ARCHAEA in the family Sulfolobaceae, found in volcanic areas where the temperature is about 80 degrees C and SULFUR is present.

Top Publications

  1. Gu Z, Zitzewitz J, Matthews C. Mapping the structure of folding cores in TIM barrel proteins by hydrogen exchange mass spectrometry: the roles of motif and sequence for the indole-3-glycerol phosphate synthase from Sulfolobus solfataricus. J Mol Biol. 2007;368:582-94 pubmed
    ..the structure of the stable folding intermediates for the of indole-3-glycerol phosphate synthase from Sulfolobus solfataricus (sIGPS)...
  2. Park H, Park J, Kang H, Cha H, Kim D, Kim J, et al. TreX from Sulfolobus solfataricus ATCC 35092 displays isoamylase and 4-alpha-glucanotransferase activities. Biosci Biotechnol Biochem. 2007;71:1348-52 pubmed
    A treX in the trehalose biosynthesis gene cluster of Sulfolobus solfataricus ATCC 35092 has been reported to produce TreX, which hydrolyzes the alpha-1,6-branch portion of amylopectin and glycogen...
  3. Williams G, Johnson K, Rudolf J, McMahon S, Carter L, Oke M, et al. Structure of the heterotrimeric PCNA from Sulfolobus solfataricus. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006;62:944-8 pubmed publisher
    ..PCNA is a homotrimer in eukaryotes, but a heterotrimer in crenarchaea such as Sulfolobus solfataricus. The three proteins are SsoPCNA1 (249 residues), SsoPCNA2 (245 residues) and SsoPCNA3 (259 residues)...
  4. Ettema T, Ahmed H, Geerling A, van der Oost J, Siebers B. The non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) of Sulfolobus solfataricus: a key-enzyme of the semi-phosphorylative branch of the Entner-Doudoroff pathway. Extremophiles. 2008;12:75-88 pubmed
    ..In Sulfolobus solfataricus two different GAP converting enzymes-classical phosphorylating GAP dehydrogenase (GAPDH) and the non-..
  5. Shang Y, Zhang X, Wang X, Guo Y, Zhang Z, Zhou Y. Biochemical characterization and mutational improvement of a thermophilic esterase from Sulfolobus solfataricus P2. Biotechnol Lett. 2010;32:1151-7 pubmed publisher
    A thermophilic esterase, SsoPEst, from Sulfolobus solfataricus P2 was cloned and expressed in E. coli AD494 (DE3). Gene sequencing indicated the encoded 353 amino acids had less than 32% identity with reported esterases...
  6. Szabó Z, Sani M, Groeneveld M, Zolghadr B, Schelert J, Albers S, et al. Flagellar motility and structure in the hyperthermoacidophilic archaeon Sulfolobus solfataricus. J Bacteriol. 2007;189:4305-9 pubmed
    ..Here, we demonstrate that the structural flagellin gene from the crenarchaeaon Sulfolobus solfataricus is highly expressed in stationary-phase-grown cells and under unfavorable nutritional conditions...
  7. Cooper C, Daugherty A, Tachdjian S, Blum P, Kelly R. Role of vapBC toxin-antitoxin loci in the thermal stress response of Sulfolobus solfataricus. Biochem Soc Trans. 2009;37:123-6 pubmed publisher
    ..The hyperthermophilic crenarchaeon Sulfolobus solfataricus encodes at least 26 vapBC (where vap is virulence-associated protein) family TA loci in its genome...
  8. Roppelt V, Klug G, Evguenieva Hackenberg E. The evolutionarily conserved subunits Rrp4 and Csl4 confer different substrate specificities to the archaeal exosome. FEBS Lett. 2010;584:2931-6 pubmed publisher
    We studied the substrate specificity of the exosome of Sulfolobus solfataricus using the catalytically active Rrp41-Rrp42-hexamer and complexes containing the RNA-binding subunits Rrp4 or Csl4...
  9. Zang H, Chowdhury G, Angel K, Harris T, Guengerich F. Translesion synthesis across polycyclic aromatic hydrocarbon diol epoxide adducts of deoxyadenosine by Sulfolobus solfataricus DNA polymerase Dpo4. Chem Res Toxicol. 2006;19:859-67 pubmed
    ..epoxide oligonucleotide derivatives were studied with the archebacterial translesion DNA polymerase Sulfolobus solfataricus Dpo4...

More Information

Publications136 found, 100 shown here

  1. Shao Y, Li H. Recognition and cleavage of a nonstructured CRISPR RNA by its processing endoribonuclease Cas6. Structure. 2013;21:385-93 pubmed
    ..The distinct RNA binding and cleavage mechanisms underline the diversity in CRISPR RNA processing. ..
  2. Maaty W, Selvig K, Ryder S, Tarlykov P, Hilmer J, Heinemann J, et al. Proteomic analysis of Sulfolobus solfataricus during Sulfolobus Turreted Icosahedral Virus infection. J Proteome Res. 2012;11:1420-32 pubmed publisher
    ..Sulfolobus Turreted Icosahedral Virus (STIV) infection of Sulfolobus solfataricus P2 was studied using 1D and 2D differential gel electrophoresis (DIGE) to measure abundance and redox ..
  3. Romano V, Napoli A, Salerno V, Valenti A, Rossi M, Ciaramella M. Lack of strand-specific repair of UV-induced DNA lesions in three genes of the archaeon Sulfolobus solfataricus. J Mol Biol. 2007;365:921-9 pubmed
    ..damage in the transcribed and non-transcribed strands of three genes of the hyperthermophilic archaeon Sulfolobus solfataricus. We found that in all three genes the two strands are repaired with the same efficiency with each other ..
  4. Chong P, Burja A, Radianingtyas H, Fazeli A, Wright P. Translational and transcriptional analysis of Sulfolobus solfataricus P2 to provide insights into alcohol and ketone utilisation. Proteomics. 2007;7:424-35 pubmed
    The potential of Sulfolobus solfataricus P2 for alcohol or ketone bioconversion was explored in this study. S. solfataricus was grown in different concentrations (0.1-0...
  5. Wu Y, Yuan S, Chen S, Wu D, Chen J, Wu J. Enhancing the production of galacto-oligosaccharides by mutagenesis of Sulfolobus solfataricus ?-galactosidase. Food Chem. 2013;138:1588-95 pubmed publisher
    ..In the present study, ?-galactosidase (LacS) from Sulfolobus solfataricus P2 was cloned and site-directed mutagenesis was performed to obtain two mutants, F359Q and F441Y...
  6. Hobel C, Albers S, Driessen A, Lupas A. The Sulfolobus solfataricus AAA protein Sso0909, a homologue of the eukaryotic ESCRT Vps4 ATPase. Biochem Soc Trans. 2008;36:94-8 pubmed publisher
    Sso0909 is a protein of the thermo-acidophilic crenarchaeon Sulfolobus solfataricus, annotated as a p60 katanin-like ATPase...
  7. Han D, Krauss G. Characterization of the endonuclease SSO2001 from Sulfolobus solfataricus P2. FEBS Lett. 2009;583:771-6 pubmed publisher
    ..We have investigated the properties of the protein SSO2001 from Sulfolobus solfataricus (Sso) P2, which is part of the cas gene cluster...
  8. Pretz M, Albers S, Schuurman Wolters G, Tampe R, Driessen A, van der Does C. Thermodynamics of the ATPase cycle of GlcV, the nucleotide-binding domain of the glucose ABC transporter of sulfolobus solfataricus. Biochemistry. 2006;45:15056-67 pubmed
    ..steps in the ATPase cycle of GlcV, the NBD of the glucose ABC transporter of the extreme thermoacidophile Sulfolobus solfataricus, were studied by isothermal titration calorimetry using the wild-type protein and two mutants, which are ..
  9. Cannio R, Catara G, Fiume I, Balestrieri M, Rossi M, Palmieri G. Identification of a cell-bound extracellular protease overproduced by Sulfolobus solfataricus in peptide-rich media. Protein Pept Lett. 2010;17:78-85 pubmed
    A new protease, named SsMTP was identified from the archeon Sulfolobus solfataricus. The enzyme is associated to the cell-membrane and over-produced in response to the peptide-enriched media...
  10. Broyde S, Wang L, Rechkoblit O, Geacintov N, Patel D. Lesion processing: high-fidelity versus lesion-bypass DNA polymerases. Trends Biochem Sci. 2008;33:209-19 pubmed publisher
    ..fragment (BF) from Bacillus stearothermophilus and in the lesion-bypass DNA polymerase IV (Dpo4) from Sulfolobus solfataricus. The tight fit of the BF polymerase around the nascent base pair contrasts with the more spacious, ..
  11. Palmieri G, Balestrieri M, Peter Katalinic J, Pohlentz G, Rossi M, Fiume I, et al. Surface-exposed glycoproteins of hyperthermophilic Sulfolobus solfataricus P2 show a common N-glycosylation profile. J Proteome Res. 2013;12:2779-90 pubmed publisher
    ..Here, the N-glycosylation pattern of cell-surface-exposed proteins of Sulfolobus solfataricus P2 were analyzed by lectin affinity purification, HPAEC-PAD, and multiple mass spectrometry-based ..
  12. Prato S, Vitale R, Contursi P, Lipps G, Saviano M, Rossi M, et al. Molecular modeling and functional characterization of the monomeric primase-polymerase domain from the Sulfolobus solfataricus plasmid pIT3. FEBS J. 2008;275:4389-402 pubmed publisher
    A tri-functional monomeric primase-polymerase domain encoded by the plasmid pIT3 from Sulfolobus solfataricus strain IT3 was identified using a structural-functional approach...
  13. Brown J, Suo Z. Elucidating the kinetic mechanism of DNA polymerization catalyzed by Sulfolobus solfataricus P2 DNA polymerase B1. Biochemistry. 2009;48:7502-11 pubmed publisher
    ..to resolve the kinetic mechanism of DNA polymerization catalyzed by an exonuclease-deficient mutant of Sulfolobus solfataricus P2 DNA polymerase B1 (PolB1 exo-)...
  14. Rothenberg E, Trakselis M, Bell S, Ha T. MCM forked substrate specificity involves dynamic interaction with the 5'-tail. J Biol Chem. 2007;282:34229-34 pubmed
    ..to probe the substrate specificity and binding mechanism of MCM from the hyperthermophilic Archaea Sulfolobus solfataricus on various DNA substrates. S...
  15. Deng L, Kenchappa C, Peng X, She Q, Garrett R. Modulation of CRISPR locus transcription by the repeat-binding protein Cbp1 in Sulfolobus. Nucleic Acids Res. 2012;40:2470-80 pubmed publisher
    ..Supporting evidence is provided by microarray and northern blotting analyses, and publicly available whole-transcriptome data for S. solfataricus P2. ..
  16. Eoff R, Irimia A, Angel K, Egli M, Guengerich F. Hydrogen bonding of 7,8-dihydro-8-oxodeoxyguanosine with a charged residue in the little finger domain determines miscoding events in Sulfolobus solfataricus DNA polymerase Dpo4. J Biol Chem. 2007;282:19831-43 pubmed
    b>Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) has been shown to catalyze bypass of 7,8-dihydro-8-oxodeoxyguanosine (8-oxoG) in a highly efficient and relatively accurate manner...
  17. Liu W, Pucci B, Rossi M, Pisani F, Ladenstein R. Structural analysis of the Sulfolobus solfataricus MCM protein N-terminal domain. Nucleic Acids Res. 2008;36:3235-43 pubmed publisher
    ..Here we report a 2.8 A crystal structure of the N-terminal domain (residues 1-268) of the Sulfolobus solfataricus MCM (Sso MCM) protein...
  18. Banerjee S, Christov P, Kozekova A, Rizzo C, Egli M, Stone M. Replication bypass of the trans-4-Hydroxynonenal-derived (6S,8R,11S)-1,N(2)-deoxyguanosine DNA adduct by the sulfolobus solfataricus DNA polymerase IV. Chem Res Toxicol. 2012;25:422-35 pubmed publisher
    ..The Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) belongs to the Y-family of error-prone polymerases...
  19. Walter P, Klein F, Lorentzen E, Ilchmann A, Klug G, Evguenieva Hackenberg E. Characterization of native and reconstituted exosome complexes from the hyperthermophilic archaeon Sulfolobus solfataricus. Mol Microbiol. 2006;62:1076-89 pubmed
    ..Exosome-like complexes were recently found in Archaea. Here we characterize the exosome of Sulfolobus solfataricus. Two exosome fractions can be discriminated by density gradient centrifugation...
  20. Mueller M, Takemasa R, Schwarz A, Atomi H, Nidetzky B. "Short-chain" alpha-1,4-glucan phosphorylase having a truncated N-terminal domain: functional expression and characterization of the enzyme from Sulfolobus solfataricus. Biochim Biophys Acta. 2009;1794:1709-14 pubmed publisher
    ..Structure-based sequence comparison of a putative phosphorylase from the thermophilic crenarchaeon Sulfolobus solfataricus (SsGP) with the well characterized GP from Escherichia coli reveals that SsGP totally lacks the otherwise ..
  21. Maaty W, Wiedenheft B, Tarlykov P, Schaff N, Heinemann J, Robison Cox J, et al. Something old, something new, something borrowed; how the thermoacidophilic archaeon Sulfolobus solfataricus responds to oxidative stress. PLoS ONE. 2009;4:e6964 pubmed publisher
    ..proteome, and chemical reactivity analyses of hydrogen peroxide (H(2)O(2)) induced oxidative stress in Sulfolobus solfataricus (P2) were conducted...
  22. Zhang J, Rouillon C, Kerou M, Reeks J, Brugger K, Graham S, et al. Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity. Mol Cell. 2012;45:303-13 pubmed publisher
    ..RNA is targeted by the CMR complex. In Sulfolobus solfataricus, this complex is composed of seven CAS protein subunits (Cmr1-7) and carries a diverse "payload" of ..
  23. Evguenieva Hackenberg E, Roppelt V, Finsterseifer P, Klug G. Rrp4 and Csl4 are needed for efficient degradation but not for polyadenylation of synthetic and natural RNA by the archaeal exosome. Biochemistry. 2008;47:13158-68 pubmed publisher
    The exosome of the archaeon Sulfolobus solfataricus is a protein complex with phosphorolytic and polyadenylating activity. Little is known about its substrates and the regulation of its functions...
  24. Barry E, McGeoch A, Kelman Z, Bell S. Archaeal MCM has separable processivity, substrate choice and helicase domains. Nucleic Acids Res. 2007;35:988-98 pubmed
    ..of the three principal structural modules of the homomultimeric MCM of the hyperthermophilic archaeon Sulfolobus solfataricus. Our results include the first analysis of the central AAA+ domain in isolation...
  25. Brewster A, Wang G, Yu X, Greenleaf W, Carazo J, Tjajadi M, et al. Crystal structure of a near-full-length archaeal MCM: functional insights for an AAA+ hexameric helicase. Proc Natl Acad Sci U S A. 2008;105:20191-6 pubmed publisher
    ..Whereas the eukaryotic complex consists of 6 homologous proteins (MCM2-7), the archaeon Sulfolobus solfataricus has only 1 MCM protein (ssoMCM), 6 subunits of which form a homohexamer. Here, we report a 4...
  26. Albers S, Birkeland N, Driessen A, Gertig S, Haferkamp P, Klenk H, et al. SulfoSYS (Sulfolobus Systems Biology): towards a silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation. Biochem Soc Trans. 2009;37:58-64 pubmed publisher
    SulfoSYS (Sulfolobus Systems Biology) focuses on the study of the CCM (central carbohydrate metabolism) of Sulfolobus solfataricus and its regulation under temperature variation at the systems level...
  27. Eoff R, Sanchez Ponce R, Guengerich F. Conformational changes during nucleotide selection by Sulfolobus solfataricus DNA polymerase Dpo4. J Biol Chem. 2009;284:21090-9 pubmed publisher
    ..Here we report on the conformational dynamics of a model Y-family polymerase Dpo4 from Sulfolobus solfataricus. Hydrogen-deuterium exchange in tandem with mass spectrometry was used to monitor changes in Dpo4 ..
  28. Gogliettino M, Balestrieri M, Cocca E, Mucerino S, Rossi M, Petrillo M, et al. Identification and characterisation of a novel acylpeptide hydrolase from Sulfolobus solfataricus: structural and functional insights. PLoS ONE. 2012;7:e37921 pubmed publisher
    A novel acylpeptide hydrolase, named APEH-3(Ss), was isolated from the hypertermophilic archaeon Sulfolobus solfataricus. APEH is a member of the prolyl oligopeptidase family which catalyzes the removal of acetylated amino acid residues ..
  29. Cobucci Ponzano B, Conte F, Benelli D, Londei P, Flagiello A, Monti M, et al. The gene of an archaeal alpha-L-fucosidase is expressed by translational frameshifting. Nucleic Acids Res. 2006;34:4258-68 pubmed
    ..We study here the mechanism of expression of a gene encoding for a alpha-l-fucosidase from the archaeon Sulfolobus solfataricus (fucA1), which is split in two open reading frames separated by a -1 frameshifting...
  30. Wu K, Lai X, Guo X, Hu J, Xiang X, Huang L. Interplay between primase and replication factor C in the hyperthermophilic archaeon Sulfolobus solfataricus. Mol Microbiol. 2007;63:826-37 pubmed
    The heterodimeric primase from the hyperthermophilic archaeon Sulfolobus solfataricus synthesizes long RNA and DNA products in vitro...
  31. L bben M, G ldenhaupt J, Zoltner M, Deigweiher K, Haebel P, Urbanke C, et al. Sulfate acts as phosphate analog on the monomeric catalytic fragment of the CPx-ATPase CopB from Sulfolobus solfataricus. J Mol Biol. 2007;369:368-85 pubmed publisher
    The crystal structure of the catalytic fragment of a Sulfolobus solfataricus P-type ATPase, CopB-B, was determined with a 2.6 A resolution...
  32. Ortmann A, Brumfield S, Walther J, McInnerney K, Brouns S, van de Werken H, et al. Transcriptome analysis of infection of the archaeon Sulfolobus solfataricus with Sulfolobus turreted icosahedral virus. J Virol. 2008;82:4874-83 pubmed publisher
    ..Following infection of Sulfolobus solfataricus strain 2-2-12 with STIV, transcription of virus genes was first detected at 8 h postinfection (p.i...
  33. Merone L, Mandrich L, Porzio E, Rossi M, Muller S, Reiter G, et al. Improving the promiscuous nerve agent hydrolase activity of a thermostable archaeal lactonase. Bioresour Technol. 2010;101:9204-12 pubmed publisher
    The thermostable Phosphotriesterase-Like Lactonase from Sulfolobus solfataricus (SsoPox) hydrolyzes lactones and, at a lower rate, neurotoxic organophosphorus compounds...
  34. Mandrich L, Pezzullo M, Rossi M, Manco G. SSoNDelta and SSoNDeltalong: two thermostable esterases from the same ORF in the archaeon Sulfolobus solfataricus?. Archaea. 2007;2:109-15 pubmed
    Previously, we reported from the Sulfolobus solfataricus open reading frame (ORF) SSO2517 the cloning, overexpression and characterization of an esterase belonging to the hormone-sensitive lipase (HSL) family and apparently having a ..
  35. Choi J, Eoff R, Pence M, Wang J, Martin M, Kim E, et al. Roles of the four DNA polymerases of the crenarchaeon Sulfolobus solfataricus and accessory proteins in DNA replication. J Biol Chem. 2011;286:31180-93 pubmed publisher
    The hyperthermophilic crenarchaeon Sulfolobus solfataricus P2 encodes three B-family DNA polymerase genes, B1 (Dpo1), B2 (Dpo2), and B3 (Dpo3), and one Y-family DNA polymerase gene, Dpo4, which are related to eukaryotic counterparts...
  36. Stolboushkina E, Nikonov S, Zelinskaya N, Arkhipova V, Nikulin A, Garber M, et al. Crystal structure of the archaeal translation initiation factor 2 in complex with a GTP analogue and Met-tRNAf(Met.). J Mol Biol. 2013;425:989-98 pubmed publisher
    ..Remarkably, the recently published 5.0-Å-resolution structure of almost the same ternary initiation complex differs dramatically from the structure presented. Reasons for this discrepancy are discussed. ..
  37. Reeks J, Graham S, Anderson L, Liu H, White M, Naismith J. Structure of the archaeal Cascade subunit Csa5: relating the small subunits of CRISPR effector complexes. RNA Biol. 2013;10:762-9 pubmed publisher
    ..Here, we report the crystal structure of the Csa5 protein from Sulfolobus solfataricus. Csa5 comprises a conserved ?-helical domain with a small insertion consisting of a weakly conserved ?-..
  38. Jensen K, Johansson E, Jensen K. Structural and enzymatic investigation of the Sulfolobus solfataricus uridylate kinase shows competitive UTP inhibition and the lack of GTP stimulation. Biochemistry. 2007;46:2745-57 pubmed publisher
    The pyrH gene encoding uridylate kinase (UMPK) from the extreme thermoacidophilic archaeon Sulfolobus solfataricus was cloned and expressed in Escherichia coli, and the enzyme (SsUMPK) was purified...
  39. Mikheikin A, Lin H, Mehta P, Jen Jacobson L, Trakselis M. A trimeric DNA polymerase complex increases the native replication processivity. Nucleic Acids Res. 2009;37:7194-205 pubmed publisher
    ..The primary DNA replication polymerase from Sulfolobus solfataricus (SsoDpo1) has been shown previously to provide the necessary polymerization speed and exonuclease ..
  40. Villafane A, Voskoboynik Y, Cuebas M, Ruhl I, Bini E. Response to excess copper in the hyperthermophile Sulfolobus solfataricus strain 98/2. Biochem Biophys Res Commun. 2009;385:67-71 pubmed publisher
    Copper is an essential micronutrient, but toxic in excess. Sulfolobus solfataricus cells have the ability to adapt to fluctuations of copper levels in their external environment...
  41. Zaparty M, Esser D, Gertig S, Haferkamp P, Kouril T, Manica A, et al. "Hot standards" for the thermoacidophilic archaeon Sulfolobus solfataricus. Extremophiles. 2010;14:119-42 pubmed publisher
    Within the archaea, the thermoacidophilic crenarchaeote Sulfolobus solfataricus has become an important model organism for physiology and biochemistry, comparative and functional genomics, as well as, more recently also for systems ..
  42. Villafane A, Voskoboynik Y, Ruhl I, Sannino D, Maezato Y, Blum P, et al. CopR of Sulfolobus solfataricus represents a novel class of archaeal-specific copper-responsive activators of transcription. Microbiology. 2011;157:2808-17 pubmed publisher
    ..Furthermore, by disruption of the ORF Sso2652 (copR) of the Sulfolobus solfataricus genome, we demonstrate that the gene encodes a transcriptional activator of the copper-transporting ..
  43. Wang J, Jiang P, Feng H, Feng Y, He Z. Three eukaryote-like Orc1/Cdc6 proteins functionally interact and mutually regulate their activities of binding to the replication origin in the hyperthermophilic archaeon Sulfolobus solfataricus P2. Biochem Biophys Res Commun. 2007;363:63-70 pubmed publisher
    The crenarchaeon Sulfolobus solfataricus has the potential to be a powerful model system to understand the central mechanism of eukaryotic DNA replication because it contains three active origins of replication and three eukaryote-like ..
  44. Limauro D, Pedone E, Galdi I, Bartolucci S. Peroxiredoxins as cellular guardians in Sulfolobus solfataricus: characterization of Bcp1, Bcp3 and Bcp4. FEBS J. 2008;275:2067-77 pubmed publisher
    ..comigratory protein (Bcp)1, Bcp3 and Bcp4] in the genome of the aerobic hyperthermophilic archaeon Sulfolobus solfataricus. Based on the cysteine residues conserved in the deduced aminoacidic sequence, Bcp1 and Bcp4 can be ..
  45. Wang Y, Musser S, Saleh S, Marnett L, Egli M, Stone M. Insertion of dNTPs opposite the 1,N2-propanodeoxyguanosine adduct by Sulfolobus solfataricus P2 DNA polymerase IV. Biochemistry. 2008;47:7322-34 pubmed publisher
    ..polymerase-DNA-dNTP complexes for three template-primer DNA sequences were determined, with the Y-family Sulfolobus solfataricus DNA polymerase IV (Dpo4), at resolutions between 2.4 and 2.7 A...
  46. Beckman J, Wang Q, Guengerich F. Kinetic analysis of correct nucleotide insertion by a Y-family DNA polymerase reveals conformational changes both prior to and following phosphodiester bond formation as detected by tryptophan fluorescence. J Biol Chem. 2008;283:36711-23 pubmed publisher
    The Sulfolobus solfataricus Y-family DNA polymerase Dpo4 is a model for translesion replication and has been used in the analysis of individual steps involved in catalysis. The role of conformational changes has not been clear...
  47. Fiorentino G, Ronca R, Cannio R, Rossi M, Bartolucci S. MarR-like transcriptional regulator involved in detoxification of aromatic compounds in Sulfolobus solfataricus. J Bacteriol. 2007;189:7351-60 pubmed publisher
    A DNA binding protein, BldR, was identified in the crenarchaeon Sulfolobus solfataricus as a protein 5- to 10-fold more abundant in cells grown in the presence of toxic aldehydes; it binds to regulatory sequences located upstream of an ..
  48. Zhang H, Eoff R, Kozekov I, Rizzo C, Egli M, Guengerich F. Versatility of Y-family Sulfolobus solfataricus DNA polymerase Dpo4 in translesion synthesis past bulky N2-alkylguanine adducts. J Biol Chem. 2009;284:3563-76 pubmed publisher
    In contrast to replicative DNA polymerases, Sulfolobus solfataricus Dpo4 showed a limited decrease in catalytic efficiency (k(cat)/Km) for insertion of dCTP opposite a series of N2-alkylguanine templates of increasing size from (methyl (..
  49. Barry E, Lovett J, Costa A, Lea S, Bell S. Intersubunit allosteric communication mediated by a conserved loop in the MCM helicase. Proc Natl Acad Sci U S A. 2009;106:1051-6 pubmed publisher
    ..We reveal that a conserved loop facilitates communication between processivity and motor tiers. Interestingly, this allostery seems to be mediated by interactions between, rather than within, individual protomers in the MCM ring. ..
  50. Huang B, Wu H, Hao N, Blombach F, van der Oost J, Li X, et al. Functional study on GTP hydrolysis by the GTP-binding protein from Sulfolobus solfataricus, a member of the HflX family. J Biochem. 2010;148:103-13 pubmed publisher
    ..SsGBP from the hyperthermophilic archaeon Sulfolobus solfataricus provided the first crystal structure of the HflX family...
  51. Maxwell B, Xu C, Suo Z. DNA lesion alters global conformational dynamics of Y-family DNA polymerase during catalysis. J Biol Chem. 2012;287:13040-7 pubmed publisher
    ..our pre-steady-state kinetic studies show that DNA polymerase IV (Dpo4), a prototype Y-family enzyme from Sulfolobus solfataricus, can bypass 8-oxoG both efficiently and faithfully...
  52. Dor A, Kilkenny M, Jones S, Oliver A, Roe S, Bell S, et al. Structure of an archaeal PCNA1-PCNA2-FEN1 complex: elucidating PCNA subunit and client enzyme specificity. Nucleic Acids Res. 2006;34:4515-26 pubmed publisher
    ..We have determined the X-ray crystal structure of the Sulfolobus solfataricus PCNA1-PCNA2 heterodimer, bound to a single copy of the flap endonuclease FEN1 at 2.9 A resolution...
  53. Chae J, Kim E, Bini E, Zylstra G. Comparative analysis of the catechol 2,3-dioxygenase gene locus in thermoacidophilic archaeon Sulfolobus solfataricus strain 98/2. Biochem Biophys Res Commun. 2007;357:815-9 pubmed publisher
    A catechol 2,3-dioxygenase (C23O) gene was found from Sulfolobus solfataricus strain 98/2. Heterologous thermophilic C23O expressed in Escherichia coli showed the highest activity against catechol and 4-chlorocatechol, and at neutral pH...
  54. Dueber E, Corn J, Bell S, Berger J. Replication origin recognition and deformation by a heterodimeric archaeal Orc1 complex. Science. 2007;317:1210-3 pubmed publisher
    ..Biochemical and comparative analyses indicate that AAA+/DNA contacts observed in the structure are dynamic and evolutionarily conserved, suggesting that the complex forms a core component of the basal initiation machinery...
  55. Guo L, Feng Y, Zhang Z, Yao H, Luo Y, Wang J, et al. Biochemical and structural characterization of Cren7, a novel chromatin protein conserved among Crenarchaea. Nucleic Acids Res. 2008;36:1129-37 pubmed publisher
    ..The solution structure and DNA-binding surface of Cren7 from the hyperthermophilic crenarchaeon Sulfolobus solfataricus were determined by NMR. The protein adopts an SH3-like fold...
  56. Peng N, Xia Q, Chen Z, Liang Y, She Q. An upstream activation element exerting differential transcriptional activation on an archaeal promoter. Mol Microbiol. 2009;74:928-39 pubmed publisher
    ..To study cis-acting elements controlling arabinose-responsive expression in archaea, the promoter of the Sulfolobus solfataricus araS gene encoding an arabinose binding protein was characterized using an Sulfolobus islandicus reporter ..
  57. Pham T, Sierocinski P, van der Oost J, Wright P. Quantitative proteomic analysis of Sulfolobus solfataricus membrane proteins. J Proteome Res. 2010;9:1165-72 pubmed publisher
    A quantitative proteomic analysis of the membrane of the archaeon Sulfolobus solfataricus P2 using iTRAQ was successfully demonstrated in this technical note...
  58. Lintner N, Kerou M, Brumfield S, Graham S, Liu H, Naismith J, et al. Structural and functional characterization of an archaeal clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for antiviral defense (CASCADE). J Biol Chem. 2011;286:21643-56 pubmed publisher
    ..Here we report the structural and functional characterization of an archaeal CASCADE (aCASCADE) from Sulfolobus solfataricus. Tagged Csa2 (Cas7) expressed in S...
  59. Xu N, Li Y, Zhao Y, Guo L, Fang Y, Zhao J, et al. Identification and characterization of small RNAs in the hyperthermophilic archaeon Sulfolobus solfataricus. PLoS ONE. 2012;7:e35306 pubmed publisher
    ..20-nt in length, including phased smRNAs and potential miRNAs, from the hyperthermophilic archaeon Sulfolobus solfataricus p2 (Ssp2) based on deep sequencing. The expression of some of the miRNA candidates in Ssp2 was confirmed...
  60. Mackay D, Botting C, Taylor G, White M. An acetylase with relaxed specificity catalyses protein N-terminal acetylation in Sulfolobus solfataricus. Mol Microbiol. 2007;64:1540-8 pubmed
    ..We demonstrate that some of the most abundant proteins present in the crenarchaeote Sulfolobus solfataricus, including subunits of the thermosome, proteosome and ribosome, are acetylated at the N-terminus...
  61. Palmieri G, Bergamo P, Luini A, Ruvo M, Gogliettino M, Langella E, et al. Acylpeptide hydrolase inhibition as targeted strategy to induce proteasomal down-regulation. PLoS ONE. 2011;6:e25888 pubmed publisher
    ..Our study supports a previously unrecognized role of APEH as a negative effector of proteasome activity by an unknown mechanism and opens new perspectives for the development of strategies aimed at modulation of cancer progression. ..
  62. Maurelli L, Giovane A, Esposito A, Moracci M, Fiume I, Rossi M, et al. Evidence that the xylanase activity from Sulfolobus solfataricus Oalpha is encoded by the endoglucanase precursor gene (sso1354) and characterization of the associated cellulase activity. Extremophiles. 2008;12:689-700 pubmed publisher
    b>Sulfolobus solfataricus strain Oalpha was previously isolated for its ability to grow on minimal medium supplemented with xylan as a carbon source...
  63. Christoffersen S, Kadziola A, Johansson E, Rasmussen M, Willemo s M, Jensen K. Structural and kinetic studies of the allosteric transition in Sulfolobus solfataricus uracil phosphoribosyltransferase: Permanent activation by engineering of the C-terminus. J Mol Biol. 2009;393:464-77 pubmed publisher
    ..The tetrameric enzyme from Sulfolobus solfataricus has a unique type of allosteric regulation by cytidine triphosphate (CTP) and guanosine triphosphate (GTP)..
  64. Maezato Y, Daugherty A, Dana K, Soo E, COOPER C, Tachdjian S, et al. VapC6, a ribonucleolytic toxin regulates thermophilicity in the crenarchaeote Sulfolobus solfataricus. RNA. 2011;17:1381-92 pubmed publisher
    ..Previous transcriptomic studies of Sulfolobus solfataricus identified vapBC6 as a heat-shock (HS)-inducible member of the Vap toxin-antitoxin gene family...
  65. V llmecke C, Drees S, Reimann J, Albers S, L bben M. The ATPases CopA and CopB both contribute to copper resistance of the thermoacidophilic archaeon Sulfolobus solfataricus. Microbiology. 2012;158:1622-33 pubmed publisher
    ..P-type ATPases of the P(IB)-subclass play a major role in metal homeostasis. The thermoacidophile Sulfolobus solfataricus possesses two P(IB)-ATPases named CopA and CopB...
  66. Fang T, Tseng W, Guo M, Shih T, Hung X. Expression, purification, and characterization of the maltooligosyltrehalose trehalohydrolase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092. J Agric Food Chem. 2006;54:7105-12 pubmed
    ..In this study, the treZ gene encoding MTHase was PCR-cloned from Sulfolobus solfataricus ATCC 35092 and then expressed in Escherichia coli...
  67. Peeters E, Wartel C, Maes D, Charlier D. Analysis of the DNA-binding sequence specificity of the archaeal transcriptional regulator Ss-LrpB from Sulfolobus solfataricus by systematic mutagenesis and high resolution contact probing. Nucleic Acids Res. 2007;35:623-33 pubmed
    ..G at position 5. The knowledge of the DNA-binding specificity will constitute a precious tool for the search of new physiologically relevant binding sites for Ss-LrpB in the genome...
  68. Robinson N, Blood K, McCallum S, Edwards P, Bell S. Sister chromatid junctions in the hyperthermophilic archaeon Sulfolobus solfataricus. EMBO J. 2007;26:816-24 pubmed
    ..In the course of our analysis of the third replication origin in the archaeon Sulfolobus solfataricus, we identify and characterise sister chromatid junctions in this prokaryote...
  69. Chen L, Ko T, Chang Y, Lin K, Chang C, Wang A, et al. Crystal structure of the left-handed archaeal RadA helical filament: identification of a functional motif for controlling quaternary structures and enzymatic functions of RecA family proteins. Nucleic Acids Res. 2007;35:1787-801 pubmed
    ..Here, we report the crystal structure of a left-handed Sulfolobus solfataricus RadA helical filament...
  70. Navarro M, Oliveira C, Zanchin N, Guimar es B. Insights into the mechanism of progressive RNA degradation by the archaeal exosome. J Biol Chem. 2008;283:14120-31 pubmed publisher
    ..Finally, comparisons between the archaeal and human exosome structures provide a possible explanation for the eukaryotic exosome inability to catalyze phosphate-dependent RNA degradation...
  71. Palmieri G, Catara G, Saviano M, Langella E, Gogliettino M, Rossi M. First Archaeal PEPB-Serine Protease Inhibitor from Sulfolobus solfataricus with Noncanonical Amino Acid Sequence in the Reactive-Site Loop. J Proteome Res. 2009;8:327-34 pubmed publisher
    ..In this study, we report the molecular cloning of the sso0767 gene from Sulfolobus solfataricus, and the functional characterization of its product, SsCEI, which represents the first archaeal ..
  72. Gogliettino M, Balestrieri M, Pocsfalvi G, Fiume I, Natale L, Rossi M, et al. A highly selective oligopeptide binding protein from the archaeon Sulfolobus solfataricus. J Bacteriol. 2010;192:3123-31 pubmed publisher
    SSO1273 of Sulfolobus solfataricus was identified as a cell surface-bound protein by a proteomics approach...
  73. Lintner N, Frankel K, Tsutakawa S, Alsbury D, Copi V, Young M, et al. The structure of the CRISPR-associated protein Csa3 provides insight into the regulation of the CRISPR/Cas system. J Mol Biol. 2011;405:939-55 pubmed publisher
    ..Here, we report the first structure of Csa3, a CRISPR-associated protein from Sulfolobus solfataricus (Sso1445), which reveals a dimeric two-domain protein...
  74. Manica A, Zebec Z, Steinkellner J, Schleper C. Unexpectedly broad target recognition of the CRISPR-mediated virus defence system in the archaeon Sulfolobus solfataricus. Nucleic Acids Res. 2013;41:10509-17 pubmed publisher
    The hyperthermophilic archaeon Sulfolobus solfataricus carries an extensive array of clustered regularly interspaced short palindromic repeats (CRISPR) systems able to mediate DNA degradation of invading genetic elements when ..
  75. Tachdjian S, Kelly R. Dynamic metabolic adjustments and genome plasticity are implicated in the heat shock response of the extremely thermoacidophilic archaeon Sulfolobus solfataricus. J Bacteriol. 2006;188:4553-9 pubmed
    Approximately one-third of the open reading frames encoded in the Sulfolobus solfataricus genome were differentially expressed within 5 min following an 80 to 90 degrees C temperature shift at pH 4.0...
  76. Schelert J, Drozda M, Dixit V, Dillman A, Blum P. Regulation of mercury resistance in the crenarchaeote Sulfolobus solfataricus. J Bacteriol. 2006;188:7141-50 pubmed publisher
    Mercuric ion, Hg(II), inactivates generalized transcription in the crenarchaeote Sulfolobus solfataricus. Metal challenge simultaneously derepresses transcription of mercuric reductase (merA) by interacting with the archaeal ..
  77. Wang Y, Duan Z, Zhu H, Guo X, Wang Z, Zhou J, et al. A novel Sulfolobus non-conjugative extrachromosomal genetic element capable of integration into the host genome and spreading in the presence of a fusellovirus. Virology. 2007;363:124-33 pubmed publisher
    ..An integrative non-conjugative extrachromosomal genetic element, denoted as pSSVi, has been isolated from a Sulfolobus solfataricus P2 strain and was characterized...
  78. Vasile F, Pechkova E, Nicolini C. Solution structure of the beta-subunit of the translation initiation factor aIF2 from archaebacteria Sulfolobus solfataricus. Proteins. 2008;70:1112-5 pubmed publisher
  79. Albers S, Driessen A. Conditions for gene disruption by homologous recombination of exogenous DNA into the Sulfolobus solfataricus genome. Archaea. 2008;2:145-9 pubmed
    ..Here we describe an optimization of the method for integration of exogenous DNA into S. solfataricus PBL 2025, an S. solfataricus 98/2 derivative, based on lactose auxotrophy that now allows for routine gene inactivation...
  80. Schlee S, Deuss M, Bruning M, Ivens A, Schwab T, Hellmann N, et al. Activation of anthranilate phosphoribosyltransferase from Sulfolobus solfataricus by removal of magnesium inhibition and acceleration of product release . Biochemistry. 2009;48:5199-209 pubmed publisher
    Anthranilate phosphoribosyltransferase from the hyperthermophilic archaeon Sulfolobus solfataricus (ssAnPRT) is encoded by the sstrpD gene and catalyzes the reaction of anthranilate (AA) with a complex of Mg(2+) and 5'-phosphoribosyl-..
  81. Gera N, Hussain M, Wright R, Rao B. Highly stable binding proteins derived from the hyperthermophilic Sso7d scaffold. J Mol Biol. 2011;409:601-16 pubmed publisher
    ..of targets can be generated through mutagenesis of the Sso7d protein from the hyperthermophilic archaeon Sulfolobus solfataricus. Sso7d is a small (~7 kDa, 63 amino acids) DNA-binding protein that lacks cysteine residues and has a ..
  82. Nguyen D, Park J, Shim J, Tran P, Oktavina E, Nguyen T, et al. Reaction kinetics of substrate transglycosylation catalyzed by TreX of Sulfolobus solfataricus and effects on glycogen breakdown. J Bacteriol. 2014;196:1941-9 pubmed publisher
    We studied the activity of a debranching enzyme (TreX) from Sulfolobus solfataricus on glycogen-mimic substrates, branched maltotetraosyl-?-cyclodextrin (Glc?-?-CD), and natural glycogen to better understand substrate transglycosylation ..
  83. Lorentzen E, Dziembowski A, Lindner D, Seraphin B, Conti E. RNA channelling by the archaeal exosome. EMBO Rep. 2007;8:470-6 pubmed
    ..Here, we present the 1.6 A resolution crystal structure of the nine-subunit exosome of Sulfolobus solfataricus and the 2...
  84. Stolboushkina E, Nikonov S, Nikulin A, Bl si U, Manstein D, Fedorov R, et al. Crystal structure of the intact archaeal translation initiation factor 2 demonstrates very high conformational flexibility in the alpha- and beta-subunits. J Mol Biol. 2008;382:680-91 pubmed publisher
    ..The crystal structure of the full-sized heterotrimeric aIF2 from Sulfolobus solfataricus in the nucleotide-free form has been determined at 2.8-A resolution...
  85. Park Y, Yoon S, Lee H. A novel thermostable arylesterase from the archaeon Sulfolobus solfataricus P1: purification, characterization, and expression. J Bacteriol. 2008;190:8086-95 pubmed publisher
    ..thermostable arylesterase, a 35-kDa monomeric enzyme, was purified from the thermoacidophilic archaeon Sulfolobus solfataricus P1. The optimum temperature and pH were 94 degrees C and 7.0, respectively...
  86. D Ambrosio K, Limauro D, Pedone E, Galdi I, Pedone C, Bartolucci S, et al. Insights into the catalytic mechanism of the Bcp family: functional and structural analysis of Bcp1 from Sulfolobus solfataricus. Proteins. 2009;76:995-1006 pubmed publisher
    ..we report on the functional and structural characterization of Bcp1, an archaeal Bcp isolated from Sulfolobus solfataricus, which presents two conserved cysteine residues at positions 45 and 50...
  87. Carter R, Drouin G. The increase in the number of subunits in eukaryotic RNA polymerase III relative to RNA polymerase II is due to the permanent recruitment of general transcription factors. Mol Biol Evol. 2010;27:1035-43 pubmed publisher
  88. Lemak S, Beloglazova N, Nocek B, Skarina T, Flick R, Brown G, et al. Toroidal structure and DNA cleavage by the CRISPR-associated [4Fe-4S] cluster containing Cas4 nuclease SSO0001 from Sulfolobus solfataricus. J Am Chem Soc. 2013;135:17476-87 pubmed publisher
    ..Here we show that the Cas4 protein SSO0001 from the archaeon Sulfolobus solfataricus has metal-dependent endonuclease and 5'?3' exonuclease activities against single-stranded DNA, as well as ..
  89. Zhang L, Rechkoblit O, Wang L, Patel D, Shapiro R, Broyde S. Mutagenic nucleotide incorporation and hindered translocation by a food carcinogen C8-dG adduct in Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): modeling and dynamics studies. Nucleic Acids Res. 2006;34:3326-37 pubmed
    ..2006), PLoS Biol., 4, e11). However, N2-dG adducts, which can reside on the minor groove side, appear to cause less hindrance when in this position...
  90. Fröls S, Gordon P, Panlilio M, Duggin I, Bell S, Sensen C, et al. Response of the hyperthermophilic archaeon Sulfolobus solfataricus to UV damage. J Bacteriol. 2007;189:8708-18 pubmed
    ..to characterize the genome-wide transcriptional response of the hyperthermophilic, aerobic crenarchaeote Sulfolobus solfataricus to UV damage, we used high-density DNA microarrays which covered 3,368 genetic features encoded on the ..
  91. Grimaldi P, Ruocco M, Lanzotti M, Ruggiero A, Ruggiero I, Arcari P, et al. Characterisation of the components of the thioredoxin system in the archaeon Sulfolobus solfataricus. Extremophiles. 2008;12:553-62 pubmed publisher
    ..We have previously characterised a Trx-B from the hyperthermophile Sulfolobus solfataricus (SsTrx-B3) (Ruocco et al. in Biochimie 86:883-892, 2004)...
  92. Iqbal J, Qureshi S. Selective depletion of Sulfolobus solfataricus transcription factor E under heat shock conditions. J Bacteriol. 2010;192:2887-91 pubmed publisher
    ..We studied the fates of various components of the Sulfolobus solfataricus transcriptional apparatus under different stresses and found that in cells incubated at 90 degrees C for ..