sulfolobus

Summary

Summary: A genus of aerobic, chemolithotrophic, coccoid ARCHAEA whose organisms are thermoacidophilic. Its cells are highly irregular in shape, often lobed, but occasionally spherical. It has worldwide distribution with organisms isolated from hot acidic soils and water. Sulfur is used as an energy source.

Top Publications

  1. Worthington P, Hoang V, Pérez Pomares F, Blum P. Targeted disruption of the alpha-amylase gene in the hyperthermophilic archaeon Sulfolobus solfataricus. J Bacteriol. 2003;185:482-8 pubmed
    b>Sulfolobus solfataricus secretes an acid-resistant alpha-amylase (amyA) during growth on starch as the sole carbon and energy source. Synthesis of this activity is subject to catabolite repression...
  2. Li Y, Sueda S, Kondo H, Kawarabayasi Y. A unique biotin carboxyl carrier protein in archaeon Sulfolobus tokodaii. FEBS Lett. 2006;580:1536-40 pubmed
    ..Archaeon Sulfolobus tokodaii is unique in that its BCCP has serine replaced for the methionine C-terminal to the lysine...
  3. Erauso G, Stedman K, van de Werken H, Zillig W, van der Oost J. Two novel conjugative plasmids from a single strain of Sulfolobus. Microbiology. 2006;152:1951-68 pubmed
    Two conjugative plasmids (CPs) were isolated and characterized from the same 'Sulfolobus islandicus' strain, SOG2/4. The plasmids were separated from each other and transferred into Sulfolobus solfataricus...
  4. Grogan D, Ozarzak M, Bernander R. Variation in gene content among geographically diverse Sulfolobus isolates. Environ Microbiol. 2008;10:137-46 pubmed
    ..We used CGH to measure relatedness of genomes drawn from Sulfolobus populations that had been shown in a previous study to be diversified along geographical lines...
  5. Cardona S, Chavez F, Jerez C. The exopolyphosphatase gene from sulfolobus solfataricus: characterization of the first gene found to be involved in polyphosphate metabolism in archaea. Appl Environ Microbiol. 2002;68:4812-9 pubmed
    ..We have cloned, sequenced, and overexpressed an exopolyphosphatase (PPX) gene (ppx) from thermophilic Sulfolobus solfataricus...
  6. Reno M, Held N, Fields C, Burke P, Whitaker R. Biogeography of the Sulfolobus islandicus pan-genome. Proc Natl Acad Sci U S A. 2009;106:8605-10 pubmed publisher
    ..Through population-scale comparative genomics of 7 Sulfolobus islandicus genomes from 3 locations, we demonstrate the biogeographical structure of the pan-genome of this ..
  7. Lou H, Duan Z, Huo X, Huang L. Modulation of hyperthermophilic DNA polymerase activity by archaeal chromatin proteins. J Biol Chem. 2004;279:127-32 pubmed
    b>Sulfolobus synthesizes a large quantity of highly conserved 7-kDa DNA-binding proteins suspected to be involved in chromosomal organization...
  8. Lundgren M, Andersson A, Chen L, Nilsson P, Bernander R. Three replication origins in Sulfolobus species: synchronous initiation of chromosome replication and asynchronous termination. Proc Natl Acad Sci U S A. 2004;101:7046-51 pubmed
    Chromosome replication origins were mapped in vivo in the two hyperthermophilic archaea, Sulfolobus acidocaldarius and Sulfolobus solfataricus, by using microarray-based marker frequency analysis...
  9. Berkner S, Lipps G. Characterization of the transcriptional activity of the cryptic plasmid pRN1 from Sulfolobus islandicus REN1H1 and regulation of its replication operon. J Bacteriol. 2007;189:1711-21 pubmed
    The plasmid pRN1 from Sulfolobus islandicus REN1H1 belongs to the crenarchaeal plasmid family pRN...

More Information

Publications131 found, 100 shown here

  1. She Q, Shen B, Chen L. Archaeal integrases and mechanisms of gene capture. Biochem Soc Trans. 2004;32:222-6 pubmed
    ..It has been demonstrated that SSV (Sulfolobus spindle virus) viruses, carrying an SSV-type integrase gene, and conjugative plasmids, carrying a pNOB8-type ..
  2. Lu S, Li Z, Wang Z, Ma X, Sheng D, Ni J, et al. Spatial subunit distribution and in vitro functions of the novel trimeric PCNA complex from Sulfolobus tokodaii. Biochem Biophys Res Commun. 2008;376:369-74 pubmed publisher
    ..The relationships among three PCNA (proliferating cell nuclear antigen) subunits in the hyperthermophilic archaeon Sulfolobus tokodaii (StoPCNAs) were analyzed and the effects of two PCNA complexes on the activities of the DNA helicase ..
  3. Dai P, Wang Y, Ye R, Chen L, Huang L. DNA topoisomerase III from the hyperthermophilic archaeon Sulfolobus solfataricus with specific DNA cleavage activity. J Bacteriol. 2003;185:5500-7 pubmed
    ..of a type IA DNA topoisomerase, previously designated topoisomerase I, from the hyperthermophilic archaeon Sulfolobus solfataricus...
  4. Fiala K, Suo Z. Pre-steady-state kinetic studies of the fidelity of Sulfolobus solfataricus P2 DNA polymerase IV. Biochemistry. 2004;43:2106-15 pubmed
    b>Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) is a thermostable archaeal enzyme and a member of the error-prone and lesion-bypass Y-family...
  5. Occhipinti E, Martelli P, Spinozzi F, Corsi F, Formantici C, Molteni L, et al. 3D structure of Sulfolobus solfataricus carboxypeptidase developed by molecular modeling is confirmed by site-directed mutagenesis and small angle X-ray scattering. Biophys J. 2003;85:1165-75 pubmed
    b>Sulfolobus solfataricus carboxypeptidase (CPSso) is a thermostable zinc-metalloenzyme with a M(r) of 43,000...
  6. Salerno V, Napoli A, White M, Rossi M, Ciaramella M. Transcriptional response to DNA damage in the archaeon Sulfolobus solfataricus. Nucleic Acids Res. 2003;31:6127-38 pubmed
    ..UV radiation induces the formation of cyclobutane pyrimidine dimers (CPDs) in the hyperthermophilic archaeon Sulfolobus solfataricus, and that these lesions are efficiently repaired in vivo in the dark, suggesting that a NER pathway ..
  7. Matoba K, Mayanagi K, Nakasu S, Kikuchi A, Morikawa K. Three-dimensional electron microscopy of the reverse gyrase from Sulfolobus tokodaii. Biochem Biophys Res Commun. 2002;297:749-55 pubmed
    ..microscopy combined with single particle analyses revealed the three-dimensional structure of the DNA-free Sulfolobus tokodaii reverse gyrase and two-dimensional average images of both the protein alone and that complexed with ..
  8. Agari Y, Sato S, Wakamatsu T, Bessho Y, Ebihara A, Yokoyama S, et al. X-ray crystal structure of a hypothetical Sua5 protein from Sulfolobus tokodaii strain 7. Proteins. 2008;70:1108-11 pubmed publisher
  9. Contursi P, Fusco S, Limauro D, Fiorentino G. Host and viral transcriptional regulators in Sulfolobus: an overview. Extremophiles. 2013;17:881-95 pubmed publisher
    The genus Sulfolobus includes microorganisms belonging to the domain Archaea, sub-kingdom Crenarchaeota, living in geographically distant acidic hot springs...
  10. Schelert J, Dixit V, Hoang V, Simbahan J, Drozda M, Blum P. Occurrence and characterization of mercury resistance in the hyperthermophilic archaeon Sulfolobus solfataricus by use of gene disruption. J Bacteriol. 2004;186:427-37 pubmed
    ..The archaeon Sulfolobus solfataricus was sensitive to mercuric chloride, and low-level adaptive resistance could be induced by metal ..
  11. Kessler A, Brinkman A, van der Oost J, Prangishvili D. Transcription of the rod-shaped viruses SIRV1 and SIRV2 of the hyperthermophilic archaeon sulfolobus. J Bacteriol. 2004;186:7745-53 pubmed
    ..of the analysis of gene expression of these viruses at different time points after infection of the host cell, Sulfolobus islandicus, and of the mapping of transcriptional start sites...
  12. Manica A, Schleper C. CRISPR-mediated defense mechanisms in the hyperthermophilic archaeal genus Sulfolobus. RNA Biol. 2013;10:671-8 pubmed publisher
    ..Archaeal genomes and, in particular, organisms of the extremely thermoacidophilic genus Sulfolobus, carry extensive CRISPR loci each with dozens of sequence signatures (spacers) able to mediate targeting and ..
  13. Wakagi T. Sulerythrin, the smallest member of the rubrerythrin family, from a strictly aerobic and thermoacidophilic archaeon, Sulfolobus tokodaii strain 7. FEMS Microbiol Lett. 2003;222:33-7 pubmed
    A protein corresponding to the N-terminal domain of rubrerythrin was isolated from a strictly aerobic archaeon, Sulfolobus tokodaii strain 7. The molecular mass was found to be 15...
  14. Kounosu A, Li Z, Cosper N, Shokes J, Scott R, Imai T, et al. Engineering a three-cysteine, one-histidine ligand environment into a new hyperthermophilic archaeal Rieske-type [2Fe-2S] ferredoxin from Sulfolobus solfataricus. J Biol Chem. 2004;279:12519-28 pubmed
    ..overproduced a hyperthermostable archaeal low potential (E(m) = -62 mV) Rieske-type ferredoxin (ARF) from Sulfolobus solfataricus strain P-1 and its variants in Escherichia coli to examine the influence of ligand substitutions on ..
  15. Portnoy V, Evguenieva Hackenberg E, Klein F, Walter P, Lorentzen E, Klug G, et al. RNA polyadenylation in Archaea: not observed in Haloferax while the exosome polynucleotidylates RNA in Sulfolobus. EMBO Rep. 2005;6:1188-93 pubmed
    ..Hyperthermophiles of the genus Sulfolobus contain a protein complex with high similarity to the exosome, which is known to degrade RNA in eukaryotes...
  16. Larson E, Reiter D, Young M, Lawrence C. Structure of A197 from Sulfolobus turreted icosahedral virus: a crenarchaeal viral glycosyltransferase exhibiting the GT-A fold. J Virol. 2006;80:7636-44 pubmed
    b>Sulfolobus turreted icosahedral virus (STIV) was the first icosahedral virus characterized from an archaeal host. It infects Sulfolobus species that thrive in the acidic hot springs (pH 2.9 to 3...
  17. Angkawidjaja C, Koga Y, Takano K, Kanaya S. Structure and stability of a thermostable carboxylesterase from the thermoacidophilic archaeon Sulfolobus tokodaii. FEBS J. 2012;279:3071-84 pubmed publisher
    ..We prepared and crystallized a carboxylesterase of the HSL family from Sulfolobus tokodaii (Sto-Est), and determined its structures in the presence and absence of an inhibitor...
  18. She Q, Zhang C, Deng L, Peng N, Chen Z, Liang Y. Genetic analyses in the hyperthermophilic archaeon Sulfolobus islandicus. Biochem Soc Trans. 2009;37:92-6 pubmed publisher
    b>Sulfolobus belongs to the hyperthermophilic archaea and it serves as a model organism to study archaeal molecular biology and evolution...
  19. Bathe S, Norris P. Ferrous iron- and sulfur-induced genes in Sulfolobus metallicus. Appl Environ Microbiol. 2007;73:2491-7 pubmed
    Genes of Sulfolobus metallicus that appeared to be upregulated in relation to growth on either ferrous iron or sulfur were identified using subtractive hybridization of cDNAs...
  20. Berkner S, Lipps G. Genetic tools for Sulfolobus spp.: vectors and first applications. Arch Microbiol. 2008;190:217-30 pubmed publisher
    b>Sulfolobus species belong to the best-studied archaeal organisms but have lacked powerful genetic methods. Recently, there has been considerable progress in the field of Sulfolobus genetics...
  21. Lipps G. Molecular biology of the pRN1 plasmid from Sulfolobus islandicus. Biochem Soc Trans. 2009;37:42-5 pubmed publisher
    The pRN1 plasmid is a rather small multicopy plasmid which was isolated from a Sulfolobus islandicus strain in 1993 by Wolfram Zillig and co-workers...
  22. Albers S, Szab Z, Driessen A. Archaeal homolog of bacterial type IV prepilin signal peptidases with broad substrate specificity. J Bacteriol. 2003;185:3918-25 pubmed
    A large number of secretory proteins in the thermoacidophile Sulfolobus solfataricus are synthesized as a precursor with an unusual leader peptide that resembles bacterial type IV prepilin signal sequences...
  23. Ellen A, Albers S, Huibers W, Pitcher A, Hobel C, Schwarz H, et al. Proteomic analysis of secreted membrane vesicles of archaeal Sulfolobus species reveals the presence of endosome sorting complex components. Extremophiles. 2009;13:67-79 pubmed publisher
    The crenarchaea Sulfolobus acidocaldarius, S. solfataricus and S. tokodaii, release membrane vesicles into the medium. These membrane vesicles consist of tetraether lipids and are coated with an S-layer...
  24. Deng L, Garrett R, Shah S, Peng X, She Q. A novel interference mechanism by a type IIIB CRISPR-Cmr module in Sulfolobus. Mol Microbiol. 2013;87:1088-99 pubmed publisher
    ..The archaeon Sulfolobus islandicus REY15A encodes three interference modules, one of type IA and two of type IIIB...
  25. Veesler D, Ng T, Sendamarai A, Eilers B, Lawrence C, Lok S, et al. Atomic structure of the 75 MDa extremophile Sulfolobus turreted icosahedral virus determined by CryoEM and X-ray crystallography. Proc Natl Acad Sci U S A. 2013;110:5504-9 pubmed publisher
    b>Sulfolobus turreted icosahedral virus (STIV) was isolated in acidic hot springs where it infects the archeon Sulfolobus solfataricus...
  26. Bini E, Dikshit V, Dirksen K, Drozda M, Blum P. Stability of mRNA in the hyperthermophilic archaeon Sulfolobus solfataricus. RNA. 2002;8:1129-36 pubmed
    ..Because archaea are generally insensitive to antibiotics, examination of mRNA stability in the hyperthermophile, Sulfolobus solfataricus, required the identification of transcription inhibitors for half-life determinations...
  27. Lipps G. Plasmids and viruses of the thermoacidophilic crenarchaeote Sulfolobus. Extremophiles. 2006;10:17-28 pubmed
    The crenarchaeote Sulfolobus spp. is a host for a remarkably large spectrum of viruses and plasmids...
  28. Larson E, Eilers B, Menon S, Reiter D, Ortmann A, Young M, et al. A winged-helix protein from Sulfolobus turreted icosahedral virus points toward stabilizing disulfide bonds in the intracellular proteins of a hyperthermophilic virus. Virology. 2007;368:249-61 pubmed
    b>Sulfolobus turreted icosahedral virus (STIV) was the first non-tailed icosahedral virus to be isolated from an archaeal host...
  29. Quax T, Krupovic M, Lucas S, Forterre P, Prangishvili D. The Sulfolobus rod-shaped virus 2 encodes a prominent structural component of the unique virion release system in Archaea. Virology. 2010;404:1-4 pubmed publisher
    ..Here we present results of a protein analysis of Sulfolobus islandicus cells infected with the rudivirus SIRV2, which enable us to postulate SIRV2-encoded protein P98 as ..
  30. Gogliettino M, Tanfani F, Scir A, Ursby T, Adinolfi B, Cacciamani T, et al. The role of Tyr41 and His155 in the functional properties of superoxide dismutase from the archaeon Sulfolobus solfataricus. Biochemistry. 2004;43:2199-208 pubmed publisher
    ..and compared the effects of mutating Y41 and H155 in the iron superoxide dismutase (SOD) from the archaeon Sulfolobus solfataricus (Ss)...
  31. Fujihashi M, Numoto N, Kobayashi Y, Mizushima A, Tsujimura M, Nakamura A, et al. Crystal structure of archaeal photolyase from Sulfolobus tokodaii with two FAD molecules: implication of a novel light-harvesting cofactor. J Mol Biol. 2007;365:903-10 pubmed publisher
    ..A similarity search of archaeal genomic data indicated the presence of a homologous gene, ST0889, on Sulfolobus tokodaii strain7. An enzymatic assay reveals that ST0889 encodes photolyase from S. tokodaii (St-photolyase)...
  32. Wagner M, Berkner S, Ajon M, Driessen A, Lipps G, Albers S. Expanding and understanding the genetic toolbox of the hyperthermophilic genus Sulfolobus. Biochem Soc Trans. 2009;37:97-101 pubmed publisher
    Although Sulfolobus species are among the best studied archaeal micro-organisms, the development and availability of genetic tools has lagged behind...
  33. Ciaramella M, Pisani F, Rossi M. Molecular biology of extremophiles: recent progress on the hyperthermophilic archaeon Sulfolobus. Antonie Van Leeuwenhoek. 2002;81:85-97 pubmed
    ..Here, we review recent insights in the molecular biology of thermoacidophilic Archaea of the genus Sulfolobus, which has been used as a model system for biochemical, structural, and genetic studies in Archaea and ..
  34. Kokoska R, McCulloch S, Kunkel T. The efficiency and specificity of apurinic/apyrimidinic site bypass by human DNA polymerase eta and Sulfolobus solfataricus Dpo4. J Biol Chem. 2003;278:50537-45 pubmed
    ..potential, here we quantify the efficiency and the specificity of AP site bypass by two Y family TLS enzymes, Sulfolobus solfataricus DNA polymerase 4 (Dpo4) and human DNA polymerase eta (Pol eta)...
  35. You D, Chon H, Koga Y, Takano K, Kanaya S. Crystallization and preliminary crystallographic analysis of type 1 RNase H from the hyperthermophilic archaeon Sulfolobus tokodaii 7. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006;62:781-4 pubmed
    Crystallization and preliminary crystallographic studies of type 1 RNase H from the hyperthermophilic archaeon Sulfolobus tokodaii 7 were performed. A crystal was grown at 277 K by the sitting-drop vapour-diffusion method...
  36. Shinkai A, Sekine S, Urushibata A, Terada T, Shirouzu M, Yokoyama S. The putative DNA-binding protein Sto12a from the thermoacidophilic archaeon Sulfolobus tokodaii contains intrachain and interchain disulfide bonds. J Mol Biol. 2007;372:1293-304 pubmed
    The Sto12a protein, from the thermoacidophilic archaeon Sulfolobus tokodaii, has been identified as a small putative DNA-binding protein...
  37. Dionne I, Nookala R, Jackson S, Doherty A, Bell S. A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus solfataricus. Mol Cell. 2003;11:275-82 pubmed
    The sliding clamp, PCNA, of the archaeon Sulfolobus solfataricus P2 is a heterotrimer of three distinct subunits (PCNA1, 2, and 3) that assembles in a defined manner...
  38. Kessler A, Sezonov G, Guijarro J, Desnoues N, Rose T, Delepierre M, et al. A novel archaeal regulatory protein, Sta1, activates transcription from viral promoters. Nucleic Acids Res. 2006;34:4837-45 pubmed
    While studying gene expression of the rudivirus SIRV1 in cells of its host, the hyperthermophilic crenarchaeon Sulfolobus, a novel archaeal transcriptional regulator was isolated...
  39. Fischer S, Maier L, Stoll B, Brendel J, Fischer E, Pfeiffer F, et al. An archaeal immune system can detect multiple protospacer adjacent motifs (PAMs) to target invader DNA. J Biol Chem. 2012;287:33351-63 pubmed
    ..encode CRISPR/Cas systems, but their molecular details have so far only been examined in three archaeal species: Sulfolobus solfataricus, Sulfolobus islandicus, and Pyrococcus furiosus...
  40. White M, Bell S. Holding it together: chromatin in the Archaea. Trends Genet. 2002;18:621-6 pubmed
    ..Indeed, it now appears that key components of the hugely complex eukaryotic chromatin regulatory machinery were established before the divergence of the archaeal and eukaryotic lineages. ..
  41. Fang T, Tseng W, Shih T, Wang M. Identification of the essential catalytic residues and selectivity-related residues of maltooligosyltrehalose trehalohydrolase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092. J Agric Food Chem. 2008;56:5628-33 pubmed publisher
  42. Ajon M, Fröls S, van Wolferen M, Stoecker K, Teichmann D, Driessen A, et al. UV-inducible DNA exchange in hyperthermophilic archaea mediated by type IV pili. Mol Microbiol. 2011;82:807-17 pubmed publisher
    ..Knockout strains of Sulfolobus acidocaldarius incapable of pilus production could not self-aggregate, but were partners in mating experiments ..
  43. Contursi P, Jensen S, Aucelli T, Rossi M, Bartolucci S, She Q. Characterization of the Sulfolobus host-SSV2 virus interaction. Extremophiles. 2006;10:615-27 pubmed
    The Sulfolobus spindle virus, SSV2, encodes a tyrosine integrase which furthers provirus formation in host chromosomes...
  44. Napoli A, Valenti A, Salerno V, Nadal M, Garnier F, Rossi M, et al. Functional interaction of reverse gyrase with single-strand binding protein of the archaeon Sulfolobus. Nucleic Acids Res. 2005;33:564-76 pubmed
    ..we show that reverse gyrase activity is stimulated by the single-strand binding protein (SSB) from the archaeon Sulfolobus solfataricus...
  45. Remonsellez F, Orell A, Jerez C. Copper tolerance of the thermoacidophilic archaeon Sulfolobus metallicus: possible role of polyphosphate metabolism. Microbiology. 2006;152:59-66 pubmed
    ..by the electron energy loss spectroscopy (EELS) procedure and quantified by using specific enzymic methods in Sulfolobus acidocaldarius, Sulfolobus metallicus and Sulfolobus solfataricus...
  46. Kuratani M, Kasai T, Akasaka R, Higashijima K, Terada T, Kigawa T, et al. Crystal structure of Sulfolobus tokodaii Sua5 complexed with L-threonine and AMPPNP. Proteins. 2011;79:2065-75 pubmed publisher
    ..In this study, we analyzed the L-threonine-binding mode of Sua5 from the archaeon Sulfolobus tokodaii. Isothermal titration calorimetry measurements revealed that S...
  47. Lai X, Shao H, Hao F, Huang L. Biochemical characterization of an ATP-dependent DNA ligase from the hyperthermophilic crenarchaeon Sulfolobus shibatae. Extremophiles. 2002;6:469-77 pubmed
    ..A gene encoding a putative ATP-dependent DNA ligase was identified in the genome of the hyperthermophilic archaeon Sulfolobus shibatae and expressed in Escherichia coli...
  48. Robinson N, Dionne I, Lundgren M, Marsh V, Bernander R, Bell S. Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricus. Cell. 2004;116:25-38 pubmed
    ..identification of two active origins of replication in the single chromosome of the hyperthermophilic archaeon Sulfolobus solfataricus...
  49. Whitaker R, Grogan D, Taylor J. Recombination shapes the natural population structure of the hyperthermophilic archaeon Sulfolobus islandicus. Mol Biol Evol. 2005;22:2354-61 pubmed
    ..We investigated the genetic structure of a thermoacidophilic crenarchaeal species, Sulfolobus islandicus, using multilocus sequence analysis of six variable protein-coding loci on a set of 60 isolates from ..
  50. Meinhart A, Blobel J, Cramer P. An extended winged helix domain in general transcription factor E/IIE alpha. J Biol Chem. 2003;278:48267-74 pubmed publisher
    ..Homology modeling shows that the TFE domain is conserved in TFIIE alpha, including the potential functional surfaces...
  51. Fushinobu S, Shoun H, Wakagi T. Crystal structure of sulerythrin, a rubrerythrin-like protein from a strictly aerobic archaeon, Sulfolobus tokodaii strain 7, shows unexpected domain swapping. Biochemistry. 2003;42:11707-15 pubmed publisher
    Sulerythrin is the first rubrerythrin-like protein to be isolated from an aerobic organism, Sulfolobus tokodaii strain 7, and it lacks a C-terminal rubredoxin-like FeS(4) domain...
  52. Peng X, Kessler A, Phan H, Garrett R, Prangishvili D. Multiple variants of the archaeal DNA rudivirus SIRV1 in a single host and a novel mechanism of genomic variation. Mol Microbiol. 2004;54:366-75 pubmed
    The DNA rudivirus SIRV1 of the hyperthermophilic archaeon Sulfolobus shows exceptional properties. Viral isolates invariably contain a population of variants with different but closely related genomes...
  53. Gardner A, Prangishvili D, Jack W. Characterization of Sulfolobus islandicus rod-shaped virus 2 gp19, a single-strand specific endonuclease. Extremophiles. 2011;15:619-24 pubmed publisher
    The hyperthermophilic Sulfolobus islandicus rod-shaped virus 2 (SIRV2) encodes a 25-kDa protein (SIRV2gp19) annotated as a hypothetical protein with sequence homology to the RecB nuclease superfamily...
  54. Wiedenheft B, Stedman K, Roberto F, Willits D, Gleske A, Zoeller L, et al. Comparative genomic analysis of hyperthermophilic archaeal Fuselloviridae viruses. J Virol. 2004;78:1954-61 pubmed
    The complete genome sequences of two Sulfolobus spindle-shaped viruses (SSVs) from acidic hot springs in Kamchatka (Russia) and Yellowstone National Park (United States) have been determined...
  55. Blount Z, Grogan D. New insertion sequences of Sulfolobus: functional properties and implications for genome evolution in hyperthermophilic archaea. Mol Microbiol. 2005;55:312-25 pubmed publisher
    ..As a way to investigate the impact of ISs on Sulfolobus genomes, we identified seven transpositionally active ISs in a widely distributed Sulfolobus species, and ..
  56. Giuliano M, Schiraldi C, Marotta M, Hugenholtz J, De Rosa M. Expression of Sulfolobus solfataricus alpha-glucosidase in Lactococcus lactis. Appl Microbiol Biotechnol. 2004;64:829-32 pubmed
    ..In this paper, the cloning of a heat-stable alpha-glucosidase from Sulfolobus solfataricus using lactic acid bacteria as expression system is reported...
  57. Theodossis A, Walden H, Westwick E, Connaris H, Lamble H, Hough D, et al. The structural basis for substrate promiscuity in 2-keto-3-deoxygluconate aldolase from the Entner-Doudoroff pathway in Sulfolobus solfataricus. J Biol Chem. 2004;279:43886-92 pubmed publisher
    The hyperthermophilic Archaea Sulfolobus solfataricus grows optimally above 80 degrees C and metabolizes glucose by a non-phosphorylative variant of the Entner-Doudoroff pathway...
  58. Zeeb M, Lipps G, Lilie H, Balbach J. Folding and association of an extremely stable dimeric protein from Sulfolobus islandicus. J Mol Biol. 2004;336:227-40 pubmed
    ORF56 is a plasmid-encoded protein from Sulfolobus islandicus, which probably controls the copy number of the pRN1 plasmid by binding to its own promotor...
  59. Andersson A, Pelve E, Lindeberg S, Lundgren M, Nilsson P, Bernander R. Replication-biased genome organisation in the crenarchaeon Sulfolobus. BMC Genomics. 2010;11:454 pubmed publisher
    Species of the crenarchaeon Sulfolobus harbour three replication origins in their single circular chromosome that are synchronously initiated during replication.
  60. Zheng T, Huang Q, Zhang C, Ni J, She Q, Shen Y. Development of a simvastatin selection marker for a hyperthermophilic acidophile, Sulfolobus islandicus. Appl Environ Microbiol. 2012;78:568-74 pubmed publisher
    We report here a novel selectable marker for the hyperthermophilic crenarchaeon Sulfolobus islandicus...
  61. Xiang X, Dong X, Huang L. Sulfolobus tengchongensis sp. nov., a novel thermoacidophilic archaeon isolated from a hot spring in Tengchong, China. Extremophiles. 2003;7:493-8 pubmed
    ..as DNA-DNA hybridization and phenotypic characterization identifies strain RT8-4 as a novel species in the genus Sulfolobus. It is proposed that strain RT8-4 be designated as Sulfolobus tengchongensis sp. nov. The type strain is RT8-4(T)...
  62. Middleton C, Parker J, Richard D, White M, Bond C. Substrate recognition and catalysis by the Holliday junction resolving enzyme Hje. Nucleic Acids Res. 2004;32:5442-51 pubmed publisher
    ..We report the high-resolution crystal structure of Hje from Sulfolobus solfataricus...
  63. Mojica F, Díez Villaseñor C, García Martínez J, Soria E. Intervening sequences of regularly spaced prokaryotic repeats derive from foreign genetic elements. J Mol Evol. 2005;60:174-82 pubmed
    ..All these biological traits could be influenced by the presence of specific spacers. CRISPR loci can be visualized as mosaics of a repeated unit, separated by sequences at some time present elsewhere in the cell...
  64. Gentile F, Amodeo P, Febbraio F, Picaro F, Motta A, Formisano S, et al. SDS-resistant active and thermostable dimers are obtained from the dissociation of homotetrameric beta-glycosidase from hyperthermophilic Sulfolobus solfataricus in SDS. Stabilizing role of the A-C intermonomeric interface. J Biol Chem. 2002;277:44050-60 pubmed
    ..Previous work with homotetrameric beta-glycosidase from hyperthermophilic Sulfolobus solfataricus (M(r) 226,760) has shown that addition of 0.05-0...
  65. Fromant M, Ferri Fioni M, Plateau P, Blanquet S. Peptidyl-tRNA hydrolase from Sulfolobus solfataricus. Nucleic Acids Res. 2003;31:3227-35 pubmed
    ..of liberating functional tRNA(Lys) from Escherichia coli diacetyl-lysyl-tRNA(Lys) was purified from the archae Sulfolobus solfataricus. Contrasting with the specificity of peptidyl- tRNA hydrolase (PTH) from E.coli, the S...
  66. Guagliardi A, Mancusi L, Rossi M. Reversion of protein aggregation mediated by Sso7d in cell extracts of Sulfolobus solfataricus. Biochem J. 2004;381:249-55 pubmed
    ..we show that stable protein aggregates can be detected in extracts of starved cells of the thermophilic archaeon Sulfolobus solfataricus, and that the protein Sso7d interacts with the aggregates and mediates the disassembly of the ..
  67. Tanaka Y, Tsumoto K, Yasutake Y, Umetsu M, Yao M, Fukada H, et al. How oligomerization contributes to the thermostability of an archaeon protein. Protein L-isoaspartyl-O-methyltransferase from Sulfolobus tokodaii. J Biol Chem. 2004;279:32957-67 pubmed publisher
    ..of archaeon proteins, we focused on a hexameric protein, protein L-isoaspartyl-O-methyltransferase from Sulfolobus tokodaii (StoPIMT)...
  68. Yamazaki S, Yoshinari S, Kita K, Watanabe Y, Kawarabayasi Y. Identification of an entire set of tRNA molecules and characterization of cleavage sites of the intron-containing tRNA precursors in acidothermophilic crenarchaeon Sulfolobus tokodaii strain7. Gene. 2011;489:103-10 pubmed publisher
    The acidothermophilic crenarchaeon, Sulfolobus tokodaii strain7, was isolated from a hot spring in Beppu, Kyushu, Japan...
  69. Kurosawa N, Itoh Y, Itoh T. Reclassification of Sulfolobus hakonensis Takayanagi et al. 1996 as Metallosphaera hakonensis comb. nov. based on phylogenetic evidence and DNA G+C content. Int J Syst Evol Microbiol. 2003;53:1607-8 pubmed
    The taxonomic status of Sulfolobus hakonensis Takayanagi et al. 1996 was re-evaluated by fresh determinations of the 16S rDNA sequence and G+C content of the genomic DNA of the type strain, HO1-1(T)...
  70. Miyakawa T, Lee W, Hatano K, Kato Y, Sawano Y, Miyazono K, et al. Crystal structure of the YjgF/YER057c/UK114 family protein from the hyperthermophilic archaeon Sulfolobus tokodaii strain 7. Proteins. 2006;62:557-61 pubmed publisher
  71. Peng X. Evidence for the horizontal transfer of an integrase gene from a fusellovirus to a pRN-like plasmid within a single strain of Sulfolobus and the implications for plasmid survival. Microbiology. 2008;154:383-91 pubmed publisher
    A fusellovirus SSV4 and a pRN-like plasmid pXZ1 were co-isolated from a single strain of Sulfolobus. In contrast to the previously characterized virus-plasmid hybrids pSSVx and pSSVi, which can coexist intracellulary with a fusellovirus, ..
  72. Ellen A, Albers S, Driessen A. Comparative study of the extracellular proteome of Sulfolobus species reveals limited secretion. Extremophiles. 2010;14:87-98 pubmed publisher
    ..A proteomic inventory and comparison of the growth medium proteins in three hyperthermoacidophiles, i.e., Sulfolobus solfataricus, S. acidocaldarius and S...
  73. Zhang C, Whitaker R. A broadly applicable gene knockout system for the thermoacidophilic archaeon Sulfolobus islandicus based on simvastatin selection. Microbiology. 2012;158:1513-22 pubmed publisher
    b>Sulfolobus species have been developed as excellent model organisms to address fundamental questions of archaeal biology...
  74. Whitaker R, Grogan D, Taylor J. Geographic barriers isolate endemic populations of hyperthermophilic archaea. Science. 2003;301:976-8 pubmed
    ..The identification of a biogeographic pattern in the archaeon Sulfolobus challenges the current model of microbial biodiversity in which unrestricted dispersal constrains the ..
  75. De Felice M, Esposito L, Pucci B, Carpentieri F, De Falco M, Rossi M, et al. Biochemical characterization of a CDC6-like protein from the crenarchaeon Sulfolobus solfataricus. J Biol Chem. 2003;278:46424-31 pubmed publisher
    ..characterization of one of the three putative Cdc6-like factors from the hyperthermophilic crenarchaeon Sulfolobus solfataricus (SsoCdc6-1)...
  76. Plakoutsi G, Taddei N, Stefani M, Chiti F. Aggregation of the Acylphosphatase from Sulfolobus solfataricus: the folded and partially unfolded states can both be precursors for amyloid formation. J Biol Chem. 2004;279:14111-9 pubmed publisher
    ..In this study, we used the acylphosphatase from the hyperthermophilic Archaea Sulfolobus solfataricus (Sso AcP) to investigate the mechanism of aggregation under conditions in which the protein ..
  77. Bernander R. The cell cycle of Sulfolobus. Mol Microbiol. 2007;66:557-62 pubmed
    Much of the current information about the archaeal cell cycle has been generated through studies of the genus Sulfolobus. The overall organization of the cell cycle in these species is well understood, and information about the regulatory ..
  78. Contursi P, Cannio R, Prato S, Fiorentino G, Rossi M, Bartolucci S. Development of a genetic system for hyperthermophilic Archaea: expression of a moderate thermophilic bacterial alcohol dehydrogenase gene in Sulfolobus solfataricus. FEMS Microbiol Lett. 2003;218:115-20 pubmed
    The Escherichia coli/Sulfolobus solfataricus shuttle vector pEXSs was used as a cloning vehicle for the gene transfer and expression of two bacterial genes in Sulfolobus solfataricus...
  79. Lou H, Duan Z, Sun T, Huang L. Cleavage of double-stranded DNA by the intrinsic 3'-5' exonuclease activity of DNA polymerase B1 from the hyperthermophilic archaeon Sulfolobus solfataricus at high temperature. FEMS Microbiol Lett. 2004;231:111-7 pubmed
    ..requirement of the intrinsic 3'-5' exonuclease of DNA polymerase B1 from the hyperthermophilic archaeon Sulfolobus solfataricus P2 (Sso polB1) was investigated...
  80. Xiang X, Chen L, Huang X, Luo Y, She Q, Huang L. Sulfolobus tengchongensis spindle-shaped virus STSV1: virus-host interactions and genomic features. J Virol. 2005;79:8677-86 pubmed
    A virus infecting the hyperthermophilic archaeon Sulfolobus tengchongensis has been isolated from a field sample from Tengchong, China, and characterized...
  81. Contursi P, Cannio R, Prato S, She Q, Rossi M, Bartolucci S. Transcriptional analysis of the genetic element pSSVx: differential and temporal regulation of gene expression reveals correlation between transcription and replication. J Bacteriol. 2007;189:6339-50 pubmed
    pSSVx from Sulfolobus islandicus strain REY15/4 is a hybrid between a plasmid and a fusellovirus...
  82. Rye C, Isupov M, Lebedev A, Littlechild J. Biochemical and structural studies of a L-haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii. Extremophiles. 2009;13:179-90 pubmed publisher
    ..The L: -2-haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii has been cloned and over-expressed in Escherichia coli and successfully purified to homogeneity...
  83. Deng L, Zhu H, Chen Z, Liang Y, She Q. Unmarked gene deletion and host-vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus. Extremophiles. 2009;13:735-46 pubmed publisher
    b>Sulfolobus islandicus is being used as a model for studying archaeal biology, geo-biology and evolution. However, no genetic system is available for this organism. To produce an S...
  84. Okada J, Okamoto T, Mukaiyama A, Tadokoro T, You D, Chon H, et al. Evolution and thermodynamics of the slow unfolding of hyperstable monomeric proteins. BMC Evol Biol. 2010;10:207 pubmed publisher
  85. Sun F, Huang L. Sulfolobus chromatin proteins modulate strand displacement by DNA polymerase B1. Nucleic Acids Res. 2013;41:8182-95 pubmed publisher
    ..We investigated the role of Sulfolobus chromatin proteins Sso7d and Cren7 in strand displacement by DNA polymerase B1 (PolB1) from the ..
  86. Middleton C, Parker J, Richard D, White M, Bond C. Crystallization and preliminary X-ray diffraction studies of Hje, a HolliDay junction resolving enzyme from Sulfolobus solfataricus. Acta Crystallogr D Biol Crystallogr. 2003;59:171-3 pubmed
    HolliDay junction endonuclease (Hje) from Sulfolobus solfataricus is a resolving enzyme involved in cleaving specific sites on either side of recombinant four-way HolliDay junctions. The HJE gene from S...
  87. Prangishvili D. Evolutionary insights from studies on viruses of hyperthermophilic archaea. Res Microbiol. 2003;154:289-94 pubmed
    ..Attempts are made to trace evolutionary relationships of viruses of hyperthermophilic archaea with other viruses...
  88. Verdon G, Albers S, van Oosterwijk N, Dijkstra B, Driessen A, Thunnissen A. Formation of the productive ATP-Mg2+-bound dimer of GlcV, an ABC-ATPase from Sulfolobus solfataricus. J Mol Biol. 2003;334:255-67 pubmed
    The ABC-ATPase GlcV from Sulfolobus solfataricus energizes an ABC transporter mediating glucose uptake...
  89. Oku Y, Ohtaki A, Kamitori S, Nakamura N, Yohda M, Ohno H, et al. Structure and direct electrochemistry of cytochrome P450 from the thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain 7. J Inorg Biochem. 2004;98:1194-9 pubmed publisher
    Cytochrome P450 from thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain 7 (P450st) has been expressed in Escherichia coli and purified at high homogeneity...
  90. De Felice M, Esposito L, Pucci B, De Falco M, Rossi M, Pisani F. A CDC6-like factor from the archaea Sulfolobus solfataricus promotes binding of the mini-chromosome maintenance complex to DNA. J Biol Chem. 2004;279:43008-12 pubmed publisher
    ..Herein, we report evidence that a Cdc6-like factor from the hyperthermophilic crenarchaea Sulfolobus solfataricus stimulates binding of the homohexameric MCM-like complex to bubble- and fork-containing DNA ..
  91. Bartolucci S, Rossi M, Cannio R. Characterization and functional complementation of a nonlethal deletion in the chromosome of a beta-glycosidase mutant of Sulfolobus solfataricus. J Bacteriol. 2003;185:3948-57 pubmed
    LacS(-) mutants of Sulfolobus solfataricus defective in beta-glycosidase activity were isolated in order to explore genomic instability and exploit novel strategies for transformation and complementation...
  92. Lamble H, Heyer N, Bull S, Hough D, Danson M. Metabolic pathway promiscuity in the archaeon Sulfolobus solfataricus revealed by studies on glucose dehydrogenase and 2-keto-3-deoxygluconate aldolase. J Biol Chem. 2003;278:34066-72 pubmed publisher
    The hyperthermophilic Archaeon Sulfolobus solfataricus metabolizes glucose by a non-phosphorylative variant of the Entner-Doudoroff pathway...