sulfolobaceae

Summary

Summary: A family of SULFOLOBALES consisting of aerobic or facultatively anaerobic chemolithotrophic cocci, usually occurring singly. They grow best at a pH of about 2.

Top Publications

  1. Kappler U, Sly L, McEwan A. Respiratory gene clusters of Metallosphaera sedula - differential expression and transcriptional organization. Microbiology. 2005;151:35-43 pubmed
    ..The data show for the first time the differential expression of the Sulfolobus-type terminal oxidase gene clusters in a Crenarchaeon in response to changing growth modes...
  2. Thauer R. Microbiology. A fifth pathway of carbon fixation. Science. 2007;318:1732-3 pubmed
  3. Berg I, Kockelkorn D, Buckel W, Fuchs G. A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea. Science. 2007;318:1782-6 pubmed publisher
    ..This indicates the importance of this enzyme in global carbon cycling...
  4. Gomes C, Teixeira M. The NADH oxidase from the thermoacidophilic archaea Acidianus ambivalens: isolation and physicochemical characterisation. Biochem Biophys Res Commun. 1998;243:412-5 pubmed
    ..As elicited by EPR, the purified enzyme also acts as an NADH:ferredoxin oxidoreductase. These features are discussed in light of the possible involvement of this protein in the metabolism of this archaea...
  5. Moll R. Protein-protein, protein-RNA and protein-lipid interactions of signal-recognition particle components in the hyperthermoacidophilic archaeon Acidianus ambivalens. Biochem J. 2003;374:247-54 pubmed
    ..However, under in vitro conditions, recombinantly generated Ffh and FtsY associate in a nucleotide-independent manner, supporting a structural receptor model with two interacting apoproteins...
  6. Hügler M, Krieger R, Jahn M, Fuchs G. Characterization of acetyl-CoA/propionyl-CoA carboxylase in Metallosphaera sedula. Carboxylating enzyme in the 3-hydroxypropionate cycle for autotrophic carbon fixation. Eur J Biochem. 2003;270:736-44 pubmed
    Autotrophic Archaea of the family Sulfolobaceae (Crenarchaeota) use a modified 3-hydroxypropionate cycle for carbon dioxide assimilation...
  7. Purschke W, Schmidt C, Petersen A, Schafer G. The terminal quinol oxidase of the hyperthermophilic archaeon Acidianus ambivalens exhibits a novel subunit structure and gene organization. J Bacteriol. 1997;179:1344-53 pubmed
    ..It is therefore concluded that A. ambivalens copes with its strongly acidic environment simply by an extreme turnover of its terminal oxidase, generating a proton gradient only by chemical charge separation...
  8. Gomes C, Teixeira M. Ambineela, an unusual blue protein isolated from the archaeon Acidianus ambivalens. Biochem Biophys Res Commun. 1998;249:23-5 pubmed
    ..Ambineela N-terminal sequence exhibits a consensus ADP-binding region, suggesting that its unknown cofactor may comprise this molecule or an analogue...
  9. Zhao L, Fang D, Shan H, Jia Y. [Effect of total solids concentration on heavy metals bioleaching from contaminated sediment]. Huan Jing Ke Xue. 2009;30:2347-52 pubmed
    ..2%, 81.2%, and 82.7%, respectively. From economical consideration, the recommended total solids concentration for the bioleaching of metals from the sediment is 10%...

More Information

Publications50

  1. Ishøy T, Kvist T, Westermann P, Ahring B. An improved method for single cell isolation of prokaryotes from meso-, thermo- and hyperthermophilic environments using micromanipulation. Appl Microbiol Biotechnol. 2006;69:510-4 pubmed
  2. Ishii M, Miyake T, Satoh T, Sugiyama H, Oshima Y, Kodama T, et al. Autotrophic carbon dioxide fixation in Acidianus brierleyi. Arch Microbiol. 1996;166:368-71 pubmed
    ..brierleyi was growing autotrophically. We conclude that a modified 3-hydroxypropionate pathway operates in A. brierleyi...
  3. Berg I, Ramos Vera W, Petri A, Huber H, Fuchs G. Study of the distribution of autotrophic CO2 fixation cycles in Crenarchaeota. Microbiology. 2010;156:256-69 pubmed publisher
    ..We conclude that autotrophic Crenarchaeota use one of the two cycles, and that their distribution correlates with the 16S rRNA-based phylogeny of this group, rather than with the aerobic or anaerobic lifestyle...
  4. Auernik K, Kelly R. Physiological versatility of the extremely thermoacidophilic archaeon Metallosphaera sedula supported by transcriptomic analysis of heterotrophic, autotrophic, and mixotrophic growth. Appl Environ Microbiol. 2010;76:931-5 pubmed publisher
    ..Routes for generation of ATP and reducing equivalents were also identified...
  5. Auernik K, Kelly R. Impact of molecular hydrogen on chalcopyrite bioleaching by the extremely thermoacidophilic archaeon Metallosphaera sedula. Appl Environ Microbiol. 2010;76:2668-72 pubmed publisher
    ..Open reading frames encoding key terminal oxidase and electron transport chain components were triggered by CuFeS(2). Evidence of heterotrophic metabolism was noted after extended periods of bioleaching, presumably related to cell lysis...
  6. Auernik K, Maezato Y, Blum P, Kelly R. The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism. Appl Environ Microbiol. 2008;74:682-92 pubmed
    ..Many hypothetical proteins were also differentially transcribed, indicating that aspects of the iron and sulfur metabolism of M. sedula remain to be identified and characterized...
  7. Giuffre A, Gomes C, Antonini G, D Itri E, Teixeira M, Brunori M. Functional properties of the quinol oxidase from Acidianus ambivalens and the possible catalytic role of its electron donor--studies on the membrane-integrated and purified enzyme. Eur J Biochem. 1997;250:383-8 pubmed
    ..We postulate that caldariella quinol, the physiological electron donor, is in vivo tightly bound to the enzyme, providing the fourth redox active center lacking in the purified enzyme...
  8. Alber B, Kung J, Fuchs G. 3-Hydroxypropionyl-coenzyme A synthetase from Metallosphaera sedula, an enzyme involved in autotrophic CO2 fixation. J Bacteriol. 2008;190:1383-9 pubmed publisher
    ..sedula, supporting its proposed role during CO2 fixation in this archaeon and possibly other members of the Sulfolobaceae family.
  9. Moriscot C, Gribaldo S, Jault J, Krupovic M, Arnaud J, Jamin M, et al. Crenarchaeal CdvA forms double-helical filaments containing DNA and interacts with ESCRT-III-like CdvB. PLoS ONE. 2011;6:e21921 pubmed publisher
    ..Here, we report the biochemical and biophysical characterization of the three Cdv proteins from the hyperthermophilic archaeon Metallospherae sedula...
  10. Mukherjee A, Wheaton G, Blum P, Kelly R. Uranium extremophily is an adaptive, rather than intrinsic, feature for extremely thermoacidophilic Metallosphaera species. Proc Natl Acad Sci U S A. 2012;109:16702-7 pubmed publisher
    ..These results point to uranium extremophily as an adaptive, rather than intrinsic, feature for Metallosphaera species, driven by environmental factors...
  11. Vestergaard G, Shah S, Bize A, Reitberger W, Reuter M, Phan H, et al. Stygiolobus rod-shaped virus and the interplay of crenarchaeal rudiviruses with the CRISPR antiviral system. J Bacteriol. 2008;190:6837-45 pubmed publisher
    ..We propose that the 12-bp indels which are commonly found in conserved rudiviral protein genes may be generated as a reaction to the presence of the host CRISPR defense system...
  12. Gomes C, Lemos R, Teixeira M, Kletzin A, Huber H, Stetter K, et al. The unusual iron sulfur composition of the Acidianus ambivalens succinate dehydrogenase complex. Biochim Biophys Acta. 1999;1411:134-41 pubmed
  13. Auernik K, Kelly R. Identification of components of electron transport chains in the extremely thermoacidophilic crenarchaeon Metallosphaera sedula through iron and sulfur compound oxidation transcriptomes. Appl Environ Microbiol. 2008;74:7723-32 pubmed publisher
    ..From this analysis, a comprehensive model for electron transport in M. sedula could be proposed as the basis for examining specific details of iron and sulfur oxidation in this bioleaching archaeon...
  14. Hawkins A, Han Y, Bennett R, Adams M, Kelly R. Role of 4-hydroxybutyrate-CoA synthetase in the CO2 fixation cycle in thermoacidophilic archaea. J Biol Chem. 2013;288:4012-22 pubmed publisher
    ..The identification of the 4-HB CoA synthetase now completes the set of enzymes comprising the 3HP/4HB cycle...
  15. Seo J, An J, Cheong J, Choi Y, Kim C. Bifunctional recombinant fusion enzyme between maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase of thermophilic microorganism Metallosphaera hakonensis. J Microbiol Biotechnol. 2008;18:1544-9 pubmed
    ..The MhMTSH fusion enzyme was active on various sizes of maltooligosaccharides, extending its substrate specificity to soluble starch, the most abundant natural source of trehalose production...
  16. Bernstein H, Beam J, Kozubal M, Carlson R, Inskeep W. In situ analysis of oxygen consumption and diffusive transport in high-temperature acidic iron-oxide microbial mats. Environ Microbiol. 2013;15:2360-70 pubmed publisher
  17. Kurosawa N, Itoh Y, Itoh T. Reclassification of Sulfolobus hakonensis Takayanagi et al. 1996 as Metallosphaera hakonensis comb. nov. based on phylogenetic evidence and DNA G+C content. Int J Syst Evol Microbiol. 2003;53:1607-8 pubmed
    ..Therefore, it is proposed that Sulfolobus hakonensis should be transferred to the genus Metallosphaera as Metallosphaera hakonensis comb. nov...
  18. Lemos R, Gomes C, Teixeira M. Acidianus ambivalens Complex II typifies a novel family of succinate dehydrogenases. Biochem Biophys Res Commun. 2001;281:141-50 pubmed
    ..An alternative membrane anchoring for this new type of SDHs, based on the amphipathic nature of the putative helices found in SdhC, is suggested...
  19. Liu L, You X, Zheng H, Wang S, Jiang C, Liu S. Complete genome sequence of Metallosphaera cuprina, a metal sulfide-oxidizing archaeon from a hot spring. J Bacteriol. 2011;193:3387-8 pubmed publisher
    ..cuprina. Genome annotation supports that M. cuprina lives a facultative life on CO(2) and organics and obtains energy from oxidation of sulfidic ores and reduced inorganic sulfuric compounds...
  20. Moczygemba C, Guidry J, Jones K, Gomes C, Teixeira M, Wittung Stafshede P. High stability of a ferredoxin from the hyperthermophilic archaeon A. ambivalens: involvement of electrostatic interactions and cofactors. Protein Sci. 2001;10:1539-48 pubmed
    ..The zinc remains bound in the unfolded state whereas the iron-sulfur clusters transiently form linear three-iron species (in pH range 2.5 to 10), which are associated with the unfolded polypeptide, before their complete degradation...
  21. Liu L, You X, Guo X, Liu S, Jiang C. Metallosphaera cuprina sp. nov., an acidothermophilic, metal-mobilizing archaeon. Int J Syst Evol Microbiol. 2011;61:2395-400 pubmed publisher
    ..The type strain is Ar-4(T) (?=?JCM 15769(T)?=?CGMCC 1.7082(T))...
  22. Sakai H, Kurosawa N. Sulfodiicoccus acidiphilus gen. nov., sp. nov., a sulfur-inhibited thermoacidophilic archaeon belonging to the order Sulfolobales isolated from a terrestrial acidic hot spring. Int J Syst Evol Microbiol. 2017;67:1880-1886 pubmed publisher
    ..basis of these results, we propose the novel genus, Sulfodiicoccus, in the order Sulfolobales (in the family Sulfolobaceae). The type species of the genus is Sulfodiicoccus acidiphilus sp. nov...
  23. McDonald J, Hall A, Gasparutto D, Cadet J, Ballantyne J, Woodgate R. Novel thermostable Y-family polymerases: applications for the PCR amplification of damaged or ancient DNAs. Nucleic Acids Res. 2006;34:1102-11 pubmed publisher
    ..The inclusion of thermostable Dpo4-like polymerases in PCRs, therefore, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications...
  24. Hügler M, Fuchs G. Assaying for the 3-hydroxypropionate cycle of carbon fixation. Methods Enzymol. 2005;397:212-21 pubmed
    ..The fate of succinyl-CoA in Sulfolobales is at issue. Assays used to study the characteristic enzymes of this novel pathway in C. aurantiacus are reported...
  25. Bandeiras T, Salgueiro C, Kletzin A, Gomes C, Teixeira M. Acidianus ambivalens type-II NADH dehydrogenase: genetic characterisation and identification of the flavin moiety as FMN. FEBS Lett. 2002;531:273-7 pubmed
    ..Further, it is suggested that membrane anchoring can be achieved via amphipatic helices...
  26. Ebara S, Shigemori Y. Alkali-tolerant high-activity catalase from a thermophilic bacterium and its overexpression in Escherichia coli. Protein Expr Purif. 2008;57:255-60 pubmed
    ..The catalase activity of the purified enzyme was optimal at a pH of 10.0. The alkali-tolerant property of this catalase makes it a promising enzyme in biotechnological applications such as H(2)O(2)-detoxifying systems...
  27. Hellwig P, Gomes C, Teixeira M. FTIR spectroscopic characterization of the cytochrome aa3 from Acidianus ambivalens: evidence for the involvement of acidic residues in redox coupled proton translocation. Biochemistry. 2003;42:6179-84 pubmed
    ..Most signals seen here are not observable in comparable spectra of typical members of the heme copper oxidase superfamily and thus reflect unique features of the enzyme from the hyperthermoacidophilic archaeon A. ambivalens...
  28. Gomes C, Bandeiras T, Teixeira M. A new type-II NADH dehydrogenase from the archaeon Acidianus ambivalens: characterization and in vitro reconstitution of the respiratory chain. J Bioenerg Biomembr. 2001;33:1-8 pubmed
    ..This system, which mimics the in vivo chain, is efficiently energized by NADH, driving oxygen consumption by means of the terminal oxidase...
  29. Maezato Y, Johnson T, McCarthy S, Dana K, Blum P. Metal resistance and lithoautotrophy in the extreme thermoacidophile Metallosphaera sedula. J Bacteriol. 2012;194:6856-63 pubmed publisher
    ..Genetic approaches provide a new means of improving the efficiency of metal bioleaching by enhancing the mechanistic understanding of thermophilic lithoautotrophy...
  30. Seo J, An J, Baik M, Park C, Cheong J, Moon T, et al. Molecular cloning and characterization of trehalose biosynthesis genes from hyperthermophilic archaebacterium Metallosphaera hakonesis. J Microbiol Biotechnol. 2007;17:123-9 pubmed
    ..The substrate specificity of the MhMTS/MhMTH-catalyzed reaction was extended to soluble starch, the most abundant maltodextrin in nature...
  31. Estelmann S, Hügler M, Eisenreich W, Werner K, Berg I, Ramos Vera W, et al. Labeling and enzyme studies of the central carbon metabolism in Metallosphaera sedula. J Bacteriol. 2011;193:1191-200 pubmed publisher
    ..This study completes the picture of the central carbon metabolism in autotrophic Sulfolobales by connecting the autotrophic carbon fixation cycle to the formation of central carbon precursor metabolites...
  32. Moll R, Schmidtke S, Sch fer G. Domain structure, GTP-hydrolyzing activity and 7S RNA binding of Acidianus ambivalens ffh-homologous protein suggest an SRP-like complex in archaea. Eur J Biochem. 1999;259:441-8 pubmed
  33. He Z, Li Y, Zhou P, Liu S. Cloning and heterologous expression of a sulfur oxygenase/reductase gene from the thermoacidophilic archaeon Acidianus sp. S5 in Escherichia coli. FEMS Microbiol Lett. 2000;193:217-21 pubmed
    ..SOR produced in E. coli catalyzes the oxidation of elemental sulfur and concomitant production of sulfite, thiosulfate and hydrogen sulfide. The recombinant enzyme exhibits the same catalytic properties as the one from Acidianus S5...
  34. Han Y, Hawkins A, Adams M, Kelly R. Epimerase (Msed_0639) and mutase (Msed_0638 and Msed_2055) convert (S)-methylmalonyl-coenzyme A (CoA) to succinyl-CoA in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate cycle. Appl Environ Microbiol. 2012;78:6194-202 pubmed publisher
    ..Based on the highly conserved occurrence of single copies of MCE and MCM in Sulfolobaceae genomes, the M...
  35. Kozubal M, Dlakic M, Macur R, Inskeep W. Terminal oxidase diversity and function in "Metallosphaera yellowstonensis": gene expression and protein modeling suggest mechanisms of Fe(II) oxidation in the sulfolobales. Appl Environ Microbiol. 2011;77:1844-53 pubmed publisher
  36. Kroeger J, Zarzycki J, Fuchs G. A spectrophotometric assay for measuring acetyl-coenzyme A carboxylase. Anal Biochem. 2011;411:100-5 pubmed publisher
    ..This assay can be adapted for high-throughput screening...
  37. Kardinahl S, Anemuller S, Schafer G. The hyper-thermostable Fe-superoxide dismutase from the Archaeon Acidianus ambivalens: characterization, recombinant expression, crystallization and effects of metal exchange. Biol Chem. 2000;381:1089-101 pubmed
    ..ambivalens is active only with iron but may incorporate other metals equally well in the catalytic center without loss of conformational stability or heat tolerance. The co-form of the enzyme could be crystallized...
  38. van der Meer M, Schouten S, Rijpstra W, Fuchs G, Sinninghe Damste J. Stable carbon isotope fractionations of the hyperthermophilic crenarchaeon Metallosphaera sedula. FEMS Microbiol Lett. 2001;196:67-70 pubmed
    ..To the best of our knowledge this is the first report of 13C fractionation effects of such a hyperthermophilic crenarchaeon...
  39. Menendez C, Bauer Z, Huber H, Gad on N, Stetter K, Fuchs G. Presence of acetyl coenzyme A (CoA) carboxylase and propionyl-CoA carboxylase in autotrophic Crenarchaeota and indication for operation of a 3-hydroxypropionate cycle in autotrophic carbon fixation. J Bacteriol. 1999;181:1088-98 pubmed
    ..The results also provide further support for the occurrence of the 3-hydroxypropionate cycle in C. aurantiacus...
  40. Alber B, Olinger M, Rieder A, Kockelkorn D, Jobst B, H gler M, et al. Malonyl-coenzyme A reductase in the modified 3-hydroxypropionate cycle for autotrophic carbon fixation in archaeal Metallosphaera and Sulfolobus spp. J Bacteriol. 2006;188:8551-9 pubmed publisher
    ..This shows that the autotrophic pathway in Chloroflexus and Sulfolobaceae has evolved convergently and that these taxonomic groups have recruited different genes to bring about similar ..
  41. Moll R, Schmidtke S, Petersen A, Schafer G. The signal recognition particle receptor alpha subunit of the hyperthermophilic archaeon Acidianus ambivalens exhibits an intrinsic GTP-hydrolyzing activity. Biochim Biophys Acta. 1997;1335:218-30 pubmed
    ..Addition of GDP greatly inhibits GTP hydrolysis in mutant and unmodified A. ambivalens SR alpha...