swine diseases

Summary

Summary: Diseases of domestic swine and of the wild boar of the genus Sus.

Webpages

  1. novartis animal health us, inc. - swine diseases hog diseases pig diseases
    www.livestock.novartis.com/swine_diseases.html
  2. swine diseases
    buchta.lib.bioinfo.pl/meid:57498
  3. swine diseases
    lib.bioinfo.pl/meid:57498
  4. novartis animal health us, inc. - cattle diseases dairy cattle diseases beef cattle diseases
    www.livestock.novartis.com/cattle_diseases.html
  5. richard l. fredrickson, veterinary diagnostic laboratory, college of veterinary medicine, u of i at illinois
    www.cvm.uiuc.edu/faculty/vdl/frdrcksn.html
  6. immunization, passive
    buchta.lib.bioinfo.pl/meid:3927
  7. novartis animal health us, inc. - cattle diseases, swine diseases, equine diseases, cattle health, bovine respiratory disease (brd), bovine viral diarrhea (bvd), calf scours, west nile virus treatment for horses
    www.livestock.novartis.com/poultry/index.html
  8. the laboratory swine - gigapedia.com
    gigapedia.org/items/101296/the-laboratory-swine
  9. swine diseases - pseudorabies
    www.vetmed.iastate.edu/departments/vdpam/swine/diseases/ches ...

Publications

  1. Effect of vaccination with selective bacterins on conventional pigs infected with type 2 porcine circovirus
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Vet Pathol 40:521-9
  2. PCR detection of porcine circovirus type 2 (PCV2) DNA in blood, tonsillar and faecal swabs from experimentally infected pigs
    A Caprioli
    Department of Veterinary Public Health and Animal Pathology, Faculty of Veterinary Medicine, Bologna University, Via Tolara di Sopra 50, 40064 Ozzano dell Emilia, BO, Italy
    Res Vet Sci 81:287-92
  3. Detection of porcine circovirus type 2 in feces of pigs with or without enteric disease by polymerase chain reaction
    Jeong S Yang
    Virology Laboratory, Department of Veterinary Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Korea
    J Vet Diagn Invest 15:369-73
  4. PCR detection of Porcine circovirus type 2 DNA in whole blood, serum, oropharyngeal swab, nasal swab, and feces from experimentally infected pigs and field cases
    Isao Shibata
    Zen-noh Institute of Animal Health, Chiba, Japan
    J Vet Med Sci 65:405-8
  5. Porcine circovirus type 2 (PCV2) vaccination of conventional pigs prevents viremia against PCV2 isolates of different genotypes and geographic origins
    M Fort
    Centre de Recerca en Sanitat Animal, Esfera UAB, Universitat Autònoma de Barcelona, Bellaterra 08193, Barcelona, Spain
    Vaccine 26:1063-71
  6. Comparative genetic characterization of Porcine Circovirus type 2 samples from German wild boar populations
    Sebastian Knell
    Department of Swine Diseases, University of Giessen, Frankfurter Strasse 112, 35392 Giessen, Germany
    Vet Microbiol 109:169-77
  7. Experimental infection of 3-week-old conventional colostrum-fed pigs with porcine circovirus type 2 and porcine parvovirus
    F Ostanello
    Department of Veterinary Public Health and Animal Pathology, Faculty of Veterinary Medicine, Bologna University, Via Tolara di Sopra 50, 40064 Ozzano Emilia BO, Italy
    Vet Microbiol 108:179-86
  8. Experimental reproduction of postweaning multisystemic wasting syndrome (PMWS) in pigs in Sweden and Denmark with a Swedish isolate of porcine circovirus type 2
    F Hasslung
    Department of Molecular Biosciences, Section of Veterinary Immunology and Virology, Swedish University of Agricultural Sciences, Biomedical Centre, PO Box 588, SE 751 23 Uppsala, Sweden
    Vet Microbiol 106:49-60
  9. Seroprevalence of porcine circovirus type 2 in swine populations in Canada and Costa Rica
    Qiang Liu
    Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon
    Can J Vet Res 66:225-31
  10. Effect of porcine parvovirus vaccination on the development of PMWS in segregated early weaned pigs coinfected with type 2 porcine circovirus and porcine parvovirus
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USA
    Vet Microbiol 98:209-20

Scientific Experts

Detail Information

Webpages15

  1. novartis animal health us, inc. - swine diseases hog diseases pig diseases
    www.livestock.novartis.com/swine_diseases.html
  2. swine diseases
    buchta.lib.bioinfo.pl/meid:57498
  3. swine diseases
    lib.bioinfo.pl/meid:57498
  4. novartis animal health us, inc. - cattle diseases dairy cattle diseases beef cattle diseases
    www.livestock.novartis.com/cattle_diseases.html
  5. richard l. fredrickson, veterinary diagnostic laboratory, college of veterinary medicine, u of i at illinois
    www.cvm.uiuc.edu/faculty/vdl/frdrcksn.html
  6. immunization, passive
    buchta.lib.bioinfo.pl/meid:3927
  7. novartis animal health us, inc. - cattle diseases, swine diseases, equine diseases, cattle health, bovine respiratory disease (brd), bovine viral diarrhea (bvd), calf scours, west nile virus treatment for horses
    www.livestock.novartis.com/poultry/index.html
  8. the laboratory swine - gigapedia.com
    gigapedia.org/items/101296/the-laboratory-swine
  9. swine diseases - pseudorabies
    www.vetmed.iastate.edu/departments/vdpam/swine/diseases/ches ...

Publications62

  1. Effect of vaccination with selective bacterins on conventional pigs infected with type 2 porcine circovirus
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Vet Pathol 40:521-9
    ..hyopneumoniae vaccines and inoculated with PCV2 compared with PCV2-inoculated unvaccinated pigs. Swine producers and veterinarians may need to consider changes in vaccination protocols in herds with recurrent PCV2-associated PMWS...
  2. PCR detection of porcine circovirus type 2 (PCV2) DNA in blood, tonsillar and faecal swabs from experimentally infected pigs
    A Caprioli
    Department of Veterinary Public Health and Animal Pathology, Faculty of Veterinary Medicine, Bologna University, Via Tolara di Sopra 50, 40064 Ozzano dell Emilia, BO, Italy
    Res Vet Sci 81:287-92
    ....
  3. Detection of porcine circovirus type 2 in feces of pigs with or without enteric disease by polymerase chain reaction
    Jeong S Yang
    Virology Laboratory, Department of Veterinary Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Korea
    J Vet Diagn Invest 15:369-73
    ..These results reveal that PCV2 is shed through the feces of pigs without clinical enteric disease, which suggests the potentiality of the fecal-oral transmission of PCV2 in feces...
  4. PCR detection of Porcine circovirus type 2 DNA in whole blood, serum, oropharyngeal swab, nasal swab, and feces from experimentally infected pigs and field cases
    Isao Shibata
    Zen-noh Institute of Animal Health, Chiba, Japan
    J Vet Med Sci 65:405-8
    ....
  5. Porcine circovirus type 2 (PCV2) vaccination of conventional pigs prevents viremia against PCV2 isolates of different genotypes and geographic origins
    M Fort
    Centre de Recerca en Sanitat Animal, Esfera UAB, Universitat Autònoma de Barcelona, Bellaterra 08193, Barcelona, Spain
    Vaccine 26:1063-71
    ..Also, the vaccine elicited PCV2-specific neutralizing antibodies to PCV2 even in the presence of maternally derived immunity...
  6. Comparative genetic characterization of Porcine Circovirus type 2 samples from German wild boar populations
    Sebastian Knell
    Department of Swine Diseases, University of Giessen, Frankfurter Strasse 112, 35392 Giessen, Germany
    Vet Microbiol 109:169-77
    ..Two further strains differed in up to 4.7% of nucleotides and up to 10.5% of amino acids (aa). These strains were aligned with clusters of PCV-2 samples from mainly French and German domestic origin...
  7. Experimental infection of 3-week-old conventional colostrum-fed pigs with porcine circovirus type 2 and porcine parvovirus
    F Ostanello
    Department of Veterinary Public Health and Animal Pathology, Faculty of Veterinary Medicine, Bologna University, Via Tolara di Sopra 50, 40064 Ozzano Emilia BO, Italy
    Vet Microbiol 108:179-86
    ..The dissemination and persistence of the virus in the tissues may depend on the level of PCV2 antibodies at the time of inoculation...
  8. Experimental reproduction of postweaning multisystemic wasting syndrome (PMWS) in pigs in Sweden and Denmark with a Swedish isolate of porcine circovirus type 2
    F Hasslung
    Department of Molecular Biosciences, Section of Veterinary Immunology and Virology, Swedish University of Agricultural Sciences, Biomedical Centre, PO Box 588, SE 751 23 Uppsala, Sweden
    Vet Microbiol 106:49-60
    ..Thus, the pathogenicity of S-PCV2 was confirmed and a role for cytokines in the etiology of PMWS was indicated...
  9. Seroprevalence of porcine circovirus type 2 in swine populations in Canada and Costa Rica
    Qiang Liu
    Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon
    Can J Vet Res 66:225-31
    ..Although these hogs were healthy when slaughtered, they were infected with PCV2 and may have previously been ill. The widespread occurrence of PCV2 in swine suggests that this virus is adapted to replication in porcine tissue...
  10. Effect of porcine parvovirus vaccination on the development of PMWS in segregated early weaned pigs coinfected with type 2 porcine circovirus and porcine parvovirus
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USA
    Vet Microbiol 98:209-20
    ..Evidence of increased incidence of clinical PMWS due to vaccination was not observed in this model...
  11. Detection of neutralizing antibodies in postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs
    Maria Fort
    Centre de Recerca en Sanitat Animal CReSA, Esfera UAB, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
    Vet Microbiol 125:244-55
    ..The increase of NA coincided with the drop in viral load. Results from this work further support that PMWS-affected pigs show an impaired humoral immune response and, particularly, an inefficient NA response against PCV2...
  12. Lack of an effect of a commercial vaccine adjuvant on the development of postweaning multisystemic wasting syndrome (PMWS) in porcine circovirus type 2 (PCV2) experimentally infected conventional pigs
    Ana Resendes
    Centre de Recerca en Sanitat Animals, Departament de Sanitat i d'Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra (Barcelona, Spain
    Vet Res 35:83-90
    ....
  13. Reproduction of postweaning multisystemic wasting syndrome (PMWS) in immunostimulated and non-immunostimulated 3-week-old piglets experimentally infected with porcine circovirus type 2 (PCV2)
    A-S Ladekjaer-Mikkelsen
    Danish Veterinary Institute, Lindholm, DK-4771 Kalvehave, Denmark
    Vet Microbiol 89:97-114
    ..In conclusion, infection with PCV2 caused PMWS in SPF piglets, however, the immunostimulation did not seem to play a critical role...
  14. Isolation and characterization of porcine circovirus type-2 from sera of stillborn fetuses
    MacDonald W Farnham
    Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, 1988 Fitch Avenue, St. Paul, Minnesota 55108, USA
    Can J Vet Res 67:108-13
    ..At present, the significance of minor differences in amino acid sequences is not known...
  15. Enhanced replication of porcine circovirus type 2 (PCV2) in a homogeneous subpopulation of PK15 cell line
    Yu Zhu
    Animal Health Biotechnology Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604, Singapore
    Virology 369:423-30
    ..These results indicate that PK15-C1 cell clone is more permissive to PCV2 infection than PK15 parent cells and thus will be useful for PCV2 replication in vitro, as well as, vaccines, diagnostic and research applications on PCV2...
  16. Serological investigation and genomic characterization of PCV2 isolates from different geographic regions of Zhejiang province in China
    J Y Zhou
    Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, Peoples Republic of China
    Vet Res Commun 30:205-20
    ..8% to 99.8% identity with PCV2 reference strains from GenBank, 76.6% to 77.9% identity with PCV1. Phylogenetic analysis indicated that there were two subgenotypes within PCV2: subgenotype I (1767 nt) and subgenotype II (1768 nt)...
  17. Excessive porcine circovirus type 2 antibody titres may trigger the development of porcine dermatitis and nephropathy syndrome: a case-control study
    G J Wellenberg
    Department of Mammalian Virology, Division of Infectious Diseases and Food Chain Quality, Institute for Animal Science and Health ID Lelystad, P O Box 65, 8200 AB Lelystad, The Netherlands
    Vet Microbiol 99:203-14
    ..The causative physiological basis for PDNS may be the excessive levels of PCV2 antibodies...
  18. The detection of porcine circovirus in the Australian pig herd
    W Raye
    School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150
    Aust Vet J 83:300-4
    ..The absence of PCV2-associated PMWS in Australia may be due to absence of essential secondary factors required for PCV2 to produce PMWS...
  19. Detection of Porcine circovirus type 2 viremia and seroconversion in naturally infected pigs in a farrow-to-finish barn
    Kathleen A McIntosh
    Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4
    Can J Vet Res 70:58-61
    ..Our results show that PCV2 viremia persists in the presence of elevated levels of PCV2-specific antibody...
  20. Serodiagnostic comparison of enzyme-linked immunosorbent assay and surface plasmon resonance for the detection of antibody to porcine circovirus type 2
    Ho-Seong Cho
    College of Veterinary Medicine, Chonnam National University, Gwangju
    Can J Vet Res 70:263-8
    ..877, P < 0.01). Therefore, this recombinant capsid protein can be used as an antigen for serologic studies, and the SPR, a label-free method, appears to be a valuable and reproducible tool in the serodiagnosis of a PCV2 infection...
  21. Differences in virulence among porcine circovirus type 2 isolates are unrelated to cluster type 2a or 2b and prior infection provides heterologous protection
    T Opriessnig
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    J Gen Virol 89:2482-91
    ..Increased virulence as reported to be associated with PCV2b isolates in the field was not observed under the conditions of this study. Moreover, cross-protection between PCV2a and PCV2b exists...
  22. [Transmission of agents of the porcine respiratory disease complex (PRDC) between swine herds: a review. Part 1--diagnosis, transmission by animal contact]
    K Woeste
    Aussenstelle für Epidemiologie, Stiftung Tierärztliche Hochschule Hannover
    Dtsch Tierarztl Wochenschr 114:324-6, 328-37
    ..In this context the limits of the interpretation of the diagnostic findings are especially described in detail. Finally, the transmission caused by pig movement is summarized in this first part of the review...
  23. Testing an ecological model for transmission of Salmonella enterica in swine production ecosystems using genotyping data
    Ronald M Weigel
    Department of Pathobiology, University of Illinois, 2001 South Lincoln Avenue, Urbana, IL 61802, USA
    Prev Vet Med 81:274-89
    ....
  24. Characterization of resistance patterns and detection of apramycin resistance genes in Escherichia coli isolated from swine exposed to various environmental conditions
    Alan G Mathew
    Department of Animal Science, University of Tennessee, 2505 River Drive, Knoxville, TN 37996, USA
    Int J Food Microbiol 89:11-20
    ..coli isotypes. Our data indicate that E. coli resistance to apramycin is increased upon exposure to various stressors...
  25. Postweaning mortality in Manitoba swine
    Cate E Dewey
    Department of Population Medicine, Ontario Veterinary College, University of Guelph
    Can J Vet Res 70:161-7
    ..These results highlight the host-agent-environment triad leading to high nursery-barn mortality rates...
  26. Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: characterization of clinical disease, virus distribution, and pathologic lesions
    M Fenaux
    Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0342, USA
    J Virol 76:541-51
    ..The data also suggest that PCV2, although essential for development of PMWS, may require other factors or agents to induce the full spectrum of clinical signs and lesions associated with advanced cases of PMWS...
  27. Prevalence of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and porcine parvovirus from aborted fetuses and pigs with respiratory problems in Korea
    K S Lyoo
    Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine, Seoul National University, Suwon 441 744, Korea
    J Vet Sci 2:201-7
    ..4%, PCV-2 46.5%, and PPV 8.1%. PCV-2 was also detected in aborted fetal tissues...
  28. A survey on finishing pig mortality associated with porcine circovirus diseases in Quebec
    Camille Moore
    Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, Québec
    Can Vet J 48:145-6
  29. Genetic characterization of porcine circovirus-2 field isolates from PMWS pigs
    Jin H Kim
    Immunopathology Laboratory, College of Veterinary Medicine, Konkuk University, Seoul, 143-701, Korea
    J Vet Sci 3:31-9
    ..Three major immunorelevant epitopes of capsid protein showed variations in amino acid sequences. Also, the variance of amino acid sequence in antigenic epitope existed between two Korean PCV-2 isolates...
  30. Postweaning multisystemic wasting syndrome of pigs in Korea: prevalence, microscopic lesions and coexisting microorganisms
    Junghyun Kim
    Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Kyouggi-Do, Republic of Korea
    J Vet Med Sci 64:57-62
    ..3%). The consistent presence of PCV-2, but lower prevalence of other viral and bacterial pathogens in all pigs examined with PMWS, has led to the speculation that PCV-2 is the etiological agent causing PMWS...
  31. Characterization of the recombinant proteins of porcine circovirus type2 field isolate expressed in the baculovirus system
    Yuna Kim
    Immnunopathology Lab College of Veterinary Medicine Konkuk University Seoul, 143-701, Korea
    J Vet Sci 3:19-23
    ..Recombinant protein expressed in the baculovirus system showed at least two glycosylation sites based on the tunicamycin treatment. Recombinant protein of the ORF2 assembled virus-like particle in recombinant virus infected insect cells...
  32. Detection of two porcine circovirus type 2 genotypic groups in United States swine herds
    A K Cheung
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa 50010, USA
    Arch Virol 152:1035-44
    ..Our analysis revealed that each genotypic group contains a distinct stretch of nucleotide or amino acid sequence that may serve as a signature motif for PCV2-group 1 or PCV2-group 2 isolates...
  33. A novel approach to intrauterine viral inoculation of swine using PCV type 2 as a model
    Kyoung Jin Yoon
    Department of Veterinary Diagnostic and Production Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Theriogenology 61:1025-37
    ..In conclusion, ultrasound needle-guided transabdominal injection was a safe and efficient method for IU inoculation of virus in pigs...
  34. Experimental reproduction of severe disease in CD/CD pigs concurrently infected with type 2 porcine circovirus and porcine reproductive and respiratory syndrome virus
    P A Harms
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA
    Vet Pathol 38:528-39
    ....
  35. Detection and genetic typing of type 2 porcine circoviruses in archived pig tissues from the UK
    S S Grierson
    Department of Virology, Veterinary Laboratories Agency Weybridge, New Haw, Addlestone, Surrey KT15 32NB
    Arch Virol 149:1171-83
    ..These data demonstrate that similar isolates of PCV-2 have been present in the UK pig population for more than 30 years...
  36. Effect of granulocyte-macrophage colony-stimulating factor on post-weaning multisystemic wasting syndrome in porcine circovirus type-2-transfected piglets
    Christophe Loizel
    The Unit of Viral Genetics and Biosafety, French Agency for Food Safety, Ploufragan, France
    Int J Exp Pathol 86:33-43
    ..GM-CSF encoding DNA injection had no significant effect on viral replication or on the production of viral particles and appearance of the disease...
  37. In vitro studies on the infection and replication of porcine circovirus type 2 in cells of the porcine immune system
    D F Gilpin
    Department of Veterinary Science, Queens University Belfast, Stoney Road, Stormont, Belfast, BT4 3SD, UK
    Vet Immunol Immunopathol 94:149-61
    ..These results suggest that in vivo, monocytic cells may not represent the primary target for PCV2 replication...
  38. Detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR
    S Cao
    Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China
    Vet Res Commun 29:263-9
    ..These results suggests that PCV-2 co-infection with PRV and PPV may play an important role in PMWS. Also, multiplex PCR is an appropriate candidate method for diagnosis of PCV-2, PRV and PPV simultaneously in field cases...
  39. Case-control study on the association of porcine circovirus type 2 and other swine viral pathogens with postweaning multisystemic wasting syndrome
    Roman M Pogranichniy
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA
    J Vet Diagn Invest 14:449-56
    ..Because PCV2 was also found in 62.5% of the controls, PCV2 from 5 cases and 4 controls were selected and genetically compared. No significant genetic difference was observed between PCV2 from PMWS and control pigs...
  40. Sequence analysis of old and new strains of porcine circovirus associated with congenital tremors in pigs and their comparison with strains involved with postweaning multisystemic wasting syndrome
    Jiwon Choi
    Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907 USA
    Can J Vet Res 66:217-24
    ..Phylogenetic analysis confirmed that the old isolate (CT-PCV-P7), and the new isolates (CT-PCV-P5, CT-PCV-P6, PMWS-PCV-P1, PMWS-PCV-P2, PMWS-PCV-P3, PMWS-PCV-P4) were correctly classified as PCV1 and PCV2, respectively...
  41. Genotyping of porcine circovirus type 2 from a variety of clinical conditions in China
    Libin Wen
    Key Laboratory of Preventive Veterinary Medicine of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100094, China
    Vet Microbiol 110:141-6
    ..It was concluded that there exist variation in ORF2 genes of different genotypic PCV2 prevailing in China...
  42. Prevalence of porcine circovirus type 2 in aborted fetuses and stillborn piglets
    J Kim
    Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, San 56-1, Shillim-dong, Kwanak-Gu 151-742, Seoul, Republic of Korea
    Vet Rec 155:489-92
    ..Viral antigen was detected in macrophages from the aborted fetuses and stillborn piglets by immunohistochemistry, and viral DNA was detected by in situ hybridisation...
  43. Differentiation of porcine circovirus 1 and 2 in formalin-fixed, paraffin-wax-embedded tissues from pigs with postweaning multisystemic wasting syndrome by in-situ hybridisation
    J Kim
    Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon, Republic of Korea
    Res Vet Sci 70:265-9
    ..In-situ hybridisation together with the differential probes developed in the present study represent an additional tool capable of differentiating of both types of PCV in formalin-fixed, paraffin-wax-embedded tissues...
  44. Genetic diversity of Brazilian strains of porcine circovirus type 2 (PCV-2) revealed by analysis of the cap gene (ORF-2)
    A M Martins Gomes de Castro
    Faculdade de Jaguariúna, Curso de Medicina Veterinária, Jaguariúna, São Paulo, Brazil
    Arch Virol 152:1435-45
    ..These results contribute to the molecular characterization of PCV-2, which serve as a basis for the epimiology of PCV-2 infection...
  45. Evidence for recombination between PCV2a and PCV2b in the field
    Richard Hesse
    Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS 66506, USA
    Virus Res 132:201-7
    ..This study demonstrates that pigs can be naturally infected with multiple PCV2 genotypes and that PCV2a/PCV2b recombination events occur in the field...
  46. Optimized protocols for the detection of porcine circovirus 2 DNA from formalin-fixed paraffin-embedded tissues using nested polymerase chain reaction and comparison of nested PCR with in situ hybridization
    J Kim
    Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Kyounggi-Do, South Korea
    J Virol Methods 92:105-11
    ..The nested PCR and in situ hybridization could be applied successfully to archival tissues for the detection of porcine circovirus 2 DNA...
  47. Multiplex PCR for rapid detection of pseudorabies virus, porcine parvovirus and porcine circoviruses
    Chienjin Huang
    Graduate Institute of Veterinary Microbiology, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC
    Vet Microbiol 101:209-14
    ..The relative efficiency (compared to performing separate assays for each virus) and apparent sensitivity of mPCR suggest its potential application for routine molecular diagnostic purposes...
  48. Quantitation of porcine circovirus type 2 isolated from serum/plasma and tissue samples of healthy pigs and pigs with postweaning multisystemic wasting syndrome using a TaqMan-based real-time PCR
    Inger Marit Brunborg
    Section for Virology and Serology, National Veterinary Institute, P O Box 8156, N 0033 Oslo, Norway
    J Virol Methods 122:171-8
    ....
  49. Multiplex nested PCR compared with in situ hybridization for the differentiation of porcine circoviruses and porcine parvovirus from pigs with postweaning multisystemic wasting syndrome
    Junghyun Kim
    Department of Veterinary Pathology, College of Veterinary Medicine, School of Agricultural Biotechnology, Seoul National University, Seoul, Kwanak-Gu 151-742, Republic of Korea
    Can J Vet Res 67:133-7
    ..Thus, multiplex nested PCR could be applied successfully to formalin-fixed, paraffin-embedded tissues for simultaneous detection of these 3 porcine viruses...
  50. Genome sequence analysis of 10 Dutch porcine circovirus type 2 (PCV-2) isolates from a PMWS case-control study
    S S Grierson
    Department of Virology, Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK
    Res Vet Sci 77:265-8
    ..These data provide further evidence to suggest that factor(s) in addition to PCV-2 are necessary in the development of PMWS...
  51. Comparison of porcine circovirus type 2 load in serum quantified by a real time PCR in postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome naturally affected pigs
    Alex Olvera
    Centre de Recerca en Sanitat Animal, Campus Universitari de Bellaterra, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
    J Virol Methods 117:75-80
    ..PDNS affected animals had lower PCV2 loads. No significant differences in viral load were found between animals suffering from PDNS and animals with mild PMWS characteristic lesions, which were unaffected clinically...
  52. Multiple porcine circovirus 2-associated abortions and reproductive failure in a multisite swine production unit
    H Gauvreau
    Prairie Diagnostic Services, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4
    Can Vet J 42:551-3
    ..Other reproductive losses and associated infectious agents in the herd are discussed...
  53. A comparison of virus isolation, polymerase chain reaction, immunohistochemistry, and in situ hybridization for the detection of porcine circovirus 2 and porcine parvovirus in experimentally and naturally coinfected pigs
    Junghyun Kim
    Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea
    J Vet Diagn Invest 16:45-50
    ....
  54. Optimal enhancement of in situ hybridization for the detection of porcine circovirus 2 in formalin-fixed, paraffin-wax-embedded tissues using a combined pretreatment of thermocycler and proteinase K
    J Kim
    Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Kwanak-Gu, 151-742 Seoul, Republic of Korea
    Res Vet Sci 74:235-40
    ..The technical improvement results, therefore, in an identical background at the same time as an increased signal and, thus, may help detect lower levels of PCV2 DNA in formalin-fixed, paraffin-wax-embedded tissues...
  55. Development and use of a multiplex real-time quantitative polymerase chain reaction assay for detection and differentiation of Porcine circovirus-2 genotypes 2a and 2b in an epidemiological survey
    Carl A Gagnon
    Faculté de Médecine Vétérinaire, Université de Montréal, 3200 rue Sicotte, St Hyacinthe, Québec, Canada
    J Vet Diagn Invest 20:545-58
    ....
  56. Porcine circovirus type 2 and associated diseases in Romania--short communication
    D Cadar
    Department of Infectious Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj Napoca, Manastur 3 5, Romania
    Acta Vet Hung 55:151-6
    ..The sequence analysis showed that the Romanian PCV2 was closely related to PCV2 identified in France and in Hungary...