malpighian tubules

Summary

Summary: Slender tubular or hairlike excretory structures found in insects. They emerge from the alimentary canal between the mesenteron (midgut) and the proctodeum (hindgut).

Top Publications

  1. Hazel M, Ianowski J, Christensen R, Maddrell S, O Donnell M. Amino acids modulate ion transport and fluid secretion by insect Malpighian tubules. J Exp Biol. 2003;206:79-91 pubmed
    ..This study shows that amino acids can modulate the secretion of ions and water by isolated Malpighian tubules of Rhodnius prolixus and Drosophila melanogaster...
  2. Lavigne C, Embleton J, Audy P, King R, Pelletier Y. Partial purification of a novel insect antidiuretic factor from the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), which acts on Malpighian tubules. Insect Biochem Mol Biol. 2001;31:339-47 pubmed
    ..factor in the head of the Colorado potato beetle (Leptinotarsa decemlineata (Say)) which acts directly on Malpighian tubules. Biologically active fractions were isolated from the head and separated using molecular weight filtration ..
  3. Naikkhwah W, O Donnell M. Salt stress alters fluid and ion transport by Malpighian tubules of Drosophila melanogaster: evidence for phenotypic plasticity. J Exp Biol. 2011;214:3443-54 pubmed publisher
    ..The results suggest that such factors stimulate tubule fluid and ion secretion through increases in intracellular Ca(2+) in response to salt stress. ..
  4. Coast G. Neuroendocrine control of ionic homeostasis in blood-sucking insects. J Exp Biol. 2009;212:378-86 pubmed publisher
    ..haematophagous insect Rhodnius prolixus is controlled by a diuretic hormone and demonstrated the role of the Malpighian tubules in meeting the volumic, osmotic and ionic challenges posed by an enormous blood meal...
  5. O Donnell M, Leader J. Changes in fluid secretion rate alter net transepithelial transport of MRP2 and P-glycoprotein substrates in Malpighian tubules of Drosophila melanogaster. Arch Insect Biochem Physiol. 2006;63:123-34 pubmed
    ..transport of the MRP2 substrate Texas Red and the p-glycoprotein substrate daunorubicin were examined in Malpighian tubules of Drosophila melanogaster...
  6. Singh S, Hou S. Lessons learned about adult kidney stem cells from the malpighian tubules of Drosophila. J Am Soc Nephrol. 2008;19:660-6 pubmed publisher
    ..The kidneys in vertebrates and the malpighian tubules in Drosophila accomplish these functions...
  7. Riegel J, Farndale R, Maddrell S. Fluid secretion by isolated Malpighian tubules of Drosophila melanogaster Meig.: effects of organic anions, quinacrine and a diuretic factor found in the secreted fluid. J Exp Biol. 1999;202:2339-48 pubmed
    ..2 and 1 mmol l(-)(1)) had no effect on the basal fluid secretion rate (FSR) of isolated Malpighian tubules of Drosophila melanogaster Meig...
  8. Eigenheer R, Nicolson S, Schegg K, Hull J, Schooley D. Identification of a potent antidiuretic factor acting on beetle Malpighian tubules. Proc Natl Acad Sci U S A. 2002;99:84-9 pubmed
    ..head extracts from the beetle Tenebrio molitor, a peptide that strongly inhibits fluid secretion by the Malpighian tubules of this insect...
  9. Secca T, Sciaccaluga M, Marra A, Barberini L, Bicchierai M. Biochemical activity and multiple locations of particulate guanylate cyclase in Rhyacophila dorsalis acutidens (Insecta: Trichoptera) provide insights into the cGMP signalling pathway in Malpighian tubules. J Insect Physiol. 2011;57:521-8 pubmed publisher
    ..for molecular physiology studies, and in other insects, cGMP and cAMP act as signalling molecules in the Malpighian tubules (MTs)...

More Information

Publications115 found, 100 shown here

  1. Campos J, Andrade C, Recco Pimentel S. Malpighian tubule polytene chromosomes of Culex quinquefasciatus (Diptera, Culicinae). Mem Inst Oswaldo Cruz. 2003;98:383-6 pubmed
    ..The use of pupal polytene chromosomes for in situ hybridization with vector competence probes is discussed. ..
  2. Terhzaz S, O Connell F, Pollock V, Kean L, Davies S, Veenstra J, et al. Isolation and characterization of a leucokinin-like peptide of Drosophila melanogaster. J Exp Biol. 1999;202:3667-76 pubmed
    ..DLK also acted to elevate intracellular [Ca(2+)] in the Malpighian tubules by approximately threefold, with an EC(50) of 10(-)(10) to 10(-)(9 )mol l(-)(1)...
  3. Giebultowicz J, Hege D. Circadian clock in Malpighian tubules. Nature. 1997;386:664 pubmed
  4. Beyenbach K, Pannabecker T, Nagel W. Central role of the apical membrane H+-ATPase in electrogenesis and epithelial transport in Malpighian tubules. J Exp Biol. 2000;203:1459-68 pubmed
    The effects of bafilomycin A(1), a blocker of V-type H(+)-ATPases, were investigated in Malpighian tubules of Aedes aegypti...
  5. O Connor K, Beyenbach K. Chloride channels in apical membrane patches of stellate cells of Malpighian tubules of Aedes aegypti. J Exp Biol. 2001;204:367-78 pubmed
    Stellate cells of Aedes aegypti Malpighian tubules were investigated using patch-clamp methods to probe the route of transepithelial Cl(-) secretion...
  6. Wiehart U, Nicolson S, Eigenheer R, Schooley D. Antagonistic control of fluid secretion by the Malpighian tubules of Tenebrio molitor: effects of diuretic and antidiuretic peptides and their second messengers. J Exp Biol. 2002;205:493-501 pubmed
    Fluid secretion by insect Malpighian tubules is controlled by haemolymph-borne factors...
  7. Santini M, Ronderos J. Allatotropin-like peptide released by Malpighian tubules induces hindgut activity associated with diuresis in the Chagas disease vector Triatoma infestans (Klug). J Exp Biol. 2007;210:1986-91 pubmed
    ..b>Malpighian tubules (MTs) have traditionally been seen as a system that responds to neuroendocrine stimulus...
  8. Leonard E, Pierce L, Gillis P, Wood C, O Donnell M. Cadmium transport by the gut and Malpighian tubules of Chironomus riparius. Aquat Toxicol. 2009;92:179-86 pubmed publisher
    ..In addition, assays using an isolated Malpighian (renal) tubule preparation have shown that the Malpighian tubules (MT) both sequester and secrete Cd...
  9. Veenstra J, Pattillo J, Petzel D. A single cDNA encodes all three Aedes leucokinins, which stimulate both fluid secretion by the malpighian tubules and hindgut contractions. J Biol Chem. 1997;272:10402-7 pubmed
    ..leucokinins 1 and 3 but not Aedes leucokinin 2 are also able to increase the rate of fluid secretion by the malpighian tubules. The differences of the three Aedes leucokinins in their potencies to induce fluid secretion or ..
  10. Pollock V, McGettigan J, Cabrero P, Maudlin I, Dow J, Davies S. Conservation of capa peptide-induced nitric oxide signalling in Diptera. J Exp Biol. 2004;207:4135-45 pubmed
    ..Finally, we show the generality of capa peptide action, to stimulate NO/cGMP signalling and increase fluid transport, across the Diptera, but not in the more primitive Orthoptera. ..
  11. Bution M, Caetano F, Zara F. Contribution of the Malpighian tubules for the maintenance of symbiotic microorganisms in cephalotes ants. Micron. 2008;39:1179-83 pubmed publisher
    Given the physiological importance of the Malpighian tubules to homeostasis in ants, this study aimed to characterize the enzymology, histology, histochemistry, and ultramorphology of the Malpighian tubules of Cephalotes atratus, C...
  12. Wessing A, Bertram G, Zierold K. Effects of bafilomycin A1 and amiloride on the apical potassium and proton gradients in Drosophila Malpighian tubules studied by X-ray microanalysis and microelectrode measurements. J Comp Physiol B. 1993;163:452-62 pubmed
    The intracellular distribution of potassium in Malpighian tubules from Drosophila larva was measured by electron probe X-ray microanalysis of freeze-dried cryosections...
  13. Karas K, Brauer P, Petzel D. Actin redistribution in mosquito malpighian tubules after a blood meal and cyclic AMP stimulation. J Insect Physiol. 2005;51:1041-54 pubmed
    Fluid secretion by mosquito Malpighian tubules is critical to maintaining fluid and electrolyte balance after a blood meal. Endogenous cAMP levels increase in Malpighian tubules after a blood meal...
  14. Ianowski J, O Donnell M. Basolateral ion transport mechanisms during fluid secretion by Drosophila Malpighian tubules: Na+ recycling, Na+:K+:2Cl- cotransport and Cl- conductance. J Exp Biol. 2004;207:2599-609 pubmed
  15. Zeng X, Singh S, Hou D, Hou S. Tumor suppressors Sav/Scrib and oncogene Ras regulate stem-cell transformation in adult Drosophila malpighian tubules. J Cell Physiol. 2010;224:766-74 pubmed publisher
    ..the oncogene Ras transform normal stem cells into CSCs through a multistep process in the adult Drosophila Malpighian Tubules (MTs). In wild-type MTs, each stem cell generates one self-renewing and one differentiating daughter cell...
  16. Gaul U, Weigel D. Regulation of Krüppel expression in the anlage of the Malpighian tubules in the Drosophila embryo. Mech Dev. 1990;33:57-67 pubmed
    ..Among other tissues, the central gap gene Krüppel is expressed and required in the anlage of the Malpighian tubules at the posterior terminus of the embryo...
  17. Kang ethe W, Aimanova K, Pullikuth A, Gill S. NHE8 mediates amiloride-sensitive Na+/H+ exchange across mosquito Malpighian tubules and catalyzes Na+ and K+ transport in reconstituted proteoliposomes. Am J Physiol Renal Physiol. 2007;292:F1501-12 pubmed
    ..Immunolocalization studies demonstrated that AeNHE8 is expressed in the apical membranes of Malpighian tubules, gastric caecae, and rectum...
  18. Singh S, Hou S. Multipotent stem cells in the Malpighian tubules of adult Drosophila melanogaster. J Exp Biol. 2009;212:413-23 pubmed publisher
    ..Excretion is performed by the kidneys in vertebrates and by Malpighian tubules (MTs) in Drosophila...
  19. Campos J, Andrade C, Recco Pimentel S. A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae). Mem Inst Oswaldo Cruz. 2003;98:387-90 pubmed
    Polytene chromosome preparations were obtained from larval, pupal and adult female Malpighian tubules of Aedes aegypti. The Malpighian tubules of the pupae (0-4 h old) from larvae reared at 20 C provided the best cytogenetic analysis...
  20. Davies S, Dow J. Modulation of epithelial innate immunity by autocrine production of nitric oxide. Gen Comp Endocrinol. 2009;162:113-21 pubmed publisher
  21. Denholm B, Sudarsan V, Pasalodos Sanchez S, Artero R, Lawrence P, Maddrell S, et al. Dual origin of the renal tubules in Drosophila: mesodermal cells integrate and polarize to establish secretory function. Curr Biol. 2003;13:1052-7 pubmed
    ..In contrast, the simple renal tubules of invertebrates, such as insect Malpighian tubules (MpTs), have always been thought to arise from single tissue primordia, epithelial buds that grow by cell ..
  22. Beyenbach K. Transport mechanisms of diuresis in Malpighian tubules of insects. J Exp Biol. 2003;206:3845-56 pubmed
    We have studied Malpighian tubules of Aedes aegypti using a variety of methods: Ramsay fluid secretion assay, electron probe analysis of secreted fluid, in vitro microperfusion and two-electrode voltage clamp...
  23. Stanisçuaski F, Te Brugge V, Carlini C, Orchard I. Invitro effect of Canavalia ensiformis urease and the derived peptide Jaburetox-2Ec on Rhodnius prolixus Malpighian tubules. J Insect Physiol. 2009;55:255-63 pubmed publisher
    ..prolixus Malpighian tubules and also investigated the involvement of second messengers...
  24. Shukla A, Tapadia M. Differential localization and processing of apoptotic proteins in Malpighian tubules of Drosophila during metamorphosis. Eur J Cell Biol. 2011;90:72-80 pubmed publisher
    ..Unlike other larval tissues, Malpighian tubules are unique in not undergoing characteristic ecdysone-induced apoptosis and are carried to the adults...
  25. Wallrath L, Burnett J, Friedman T. Molecular characterization of the Drosophila melanogaster urate oxidase gene, an ecdysone-repressible gene expressed only in the malpighian tubules. Mol Cell Biol. 1990;10:5114-27 pubmed
    ..is expressed during the third-instar larval and adult stages, exclusively within a subset of cells of the Malpighian tubules. The UO gene contains a 69-base-pair intron and encodes mature mRNAs of 1,224, 1,227, and 1,244 nucleotides, ..
  26. Davies S, Huesmann G, Maddrell S, O Donnell M, Skaer N, Dow J, et al. CAP2b, a cardioacceleratory peptide, is present in Drosophila and stimulates tubule fluid secretion via cGMP. Am J Physiol. 1995;269:R1321-6 pubmed
    ..peptide, CAP2b, identified originally in the lepidopteran Manduca sexta, stimulates fluid secretion by Malpighian tubules of the dipteran Drosophila melanogaster. High-performance liquid chromatography analyses of adult D...
  27. Kean L, Cazenave W, Costes L, Broderick K, Graham S, Pollock V, et al. Two nitridergic peptides are encoded by the gene capability in Drosophila melanogaster. Am J Physiol Regul Integr Comp Physiol. 2002;282:R1297-307 pubmed
    ..The actions of capa-1 and capa-2 are not synergistic, implying that both act on the same pathways in tubules. The capa gene is thus the first to be shown to encode neuropeptides that act on renal fluid production through nitric oxide. ..
  28. Chahine S, O Donnell M. Effects of acute or chronic exposure to dietary organic anions on secretion of methotrexate and salicylate by Malpighian tubules of Drosophila melanogaster larvae. Arch Insect Biochem Physiol. 2010;73:128-47 pubmed publisher
    The effects of dietary exposure to organic anions on the physiology of isolated Malpighian tubules and on tubule gene expression were examined using larvae of Drosophila melanogaster...
  29. Denholm B, Brown S, Ray R, Ruiz Gomez M, Skaer H, Hombria J. crossveinless-c is a RhoGAP required for actin reorganisation during morphogenesis. Development. 2005;132:2389-400 pubmed
    ..In the absence of cv-c activity, tubulogenesis in the renal or Malpighian tubules fails and they collapse into a cyst-like sack...
  30. McGettigan J, McLennan R, Broderick K, Kean L, Allan A, Cabrero P, et al. Insect renal tubules constitute a cell-autonomous immune system that protects the organism against bacterial infection. Insect Biochem Mol Biol. 2005;35:741-54 pubmed
    ..coli challenge. Thus, we describe a novel role of Malpighian tubules in immune sensing and insect survival.
  31. Leader J, O Donnell M. Transepithelial transport of fluorescent p-glycoprotein and MRP2 substrates by insect Malpighian tubules: confocal microscopic analysis of secreted fluid droplets. J Exp Biol. 2005;208:4363-76 pubmed
    ..substrates of p-glycoprotein (P-gp) and multidrug resistance-associated protein 2 (MRP2) by insect Malpighian tubules was examined using confocal laser scanning microscopy (CLSM)...
  32. Lu H, Kersch C, Pietrantonio P. The kinin receptor is expressed in the Malpighian tubule stellate cells in the mosquito Aedes aegypti (L.): a new model needed to explain ion transport?. Insect Biochem Mol Biol. 2011;41:135-40 pubmed publisher
    ..These results indicate that the currently accepted model for chloride transport must be re-evaluated in Ae. aegypti and suggest the kinin regulatory signals controlling intercellular junctions originate in the stellate cells. ..
  33. Davies S, Overend G, Sebastian S, Cundall M, Cabrero P, Dow J, et al. Immune and stress response 'cross-talk' in the Drosophila Malpighian tubule. J Insect Physiol. 2012;58:488-97 pubmed publisher
    ..Importantly, as tubule function is central to survival, they are potentially key targets for insect control, which will be facilitated by increased understanding of the complexities of stress signalling in the organism. ..
  34. Singh S, Liu W, Hou S. The adult Drosophila malpighian tubules are maintained by multipotent stem cells. Cell Stem Cell. 2007;1:191-203 pubmed publisher
    ..Excretion is performed by the kidney in vertebrates and by the Malpighian tubules in Drosophila...
  35. Chahine S, O Donnell M. Physiological and molecular characterization of methotrexate transport by Malpighian tubules of adult Drosophila melanogaster. J Insect Physiol. 2009;55:927-35 pubmed publisher
    ..and regulation of transepithelial transport of the type II organic anion methotrexate (MTX) by the Malpighian tubules of Drosophila melanogaster...
  36. Terhzaz S, Finlayson A, Stirrat L, Yang J, Tricoire H, Woods D, et al. Cell-specific inositol 1,4,5 trisphosphate 3-kinase mediates epithelial cell apoptosis in response to oxidative stress in Drosophila. Cell Signal. 2010;22:737-48 pubmed publisher
    ..Finally, targeted over-expression of either debcl or buffy to tubule principal cells does not impact on tubule caspase-9 activity. Thus, IP(3)K-1 modulates epithelial cell apoptosis without involvement of bcl-2-type proteins. ..
  37. Schepel S, Fox A, Miyauchi J, Sou T, Yang J, Lau K, et al. The single kinin receptor signals to separate and independent physiological pathways in Malpighian tubules of the yellow fever mosquito. Am J Physiol Regul Integr Comp Physiol. 2010;299:R612-22 pubmed publisher
    ..the cockroach Leucophaea (the leucokinins) to evaluate the mechanism of diuretic action of kinin peptides in Malpighian tubules of the yellow fever mosquito Aedes aegypti...
  38. Dow J, Davies S, Guo Y, Graham S, Finbow M, Kaiser K. Molecular genetic analysis of V-ATPase function in Drosophila melanogaster. J Exp Biol. 1997;200:237-45 pubmed
    ..These results, while providing the first gene knockouts of V-ATPases in animals, also illustrate the general utility of D. melanogaster as a model for the genetic analysis of ion transport and its control in epithelia. ..
  39. Broderick K, Kean L, Dow J, Pyne N, Davies S. Ectopic expression of bovine type 5 phosphodiesterase confers a renal phenotype in Drosophila. J Biol Chem. 2004;279:8159-68 pubmed
    ..The transgene also provides a generic tool for the analysis of cGMP signaling in Drosophila. ..
  40. Cabrero P, Pollock V, Davies S, Dow J. A conserved domain of alkaline phosphatase expression in the Malpighian tubules of dipteran insects. J Exp Biol. 2004;207:3299-305 pubmed
    ..The orthodoxy that stellate cells are unique to, and general among, Diptera may thus require revision. ..
  41. Coast G, Webster S, Schegg K, Tobe S, Schooley D. The Drosophila melanogaster homologue of an insect calcitonin-like diuretic peptide stimulates V-ATPase activity in fruit fly Malpighian tubules. J Exp Biol. 2001;204:1795-804 pubmed
    ..melanogaster DH31; Drome-DH31) was synthesised and tested for activity on fruit fly Malpighian tubules. It increases tubule secretion by approximately 35 % of the response obtained with a myokinin from the ..
  42. Johnson E, Shafer O, Trigg J, Park J, Schooley D, Dow J, et al. A novel diuretic hormone receptor in Drosophila: evidence for conservation of CGRP signaling. J Exp Biol. 2005;208:1239-46 pubmed
    ..CG17415 antibodies revealed expression within the principal cells of Malpighian tubules, further implicating DH31 as a ligand for this receptor...
  43. Santini M, Ronderos J. Allatotropin-like peptide in Malpighian tubules: insect renal tubules as an autonomous endocrine organ. Gen Comp Endocrinol. 2009;160:243-9 pubmed publisher
    b>Malpighian tubules (MTs) are recognised as the main excretory organ in insects, ensuring water and mineral balance...
  44. Dow J, Romero M. Drosophila provides rapid modeling of renal development, function, and disease. Am J Physiol Renal Physiol. 2010;299:F1237-44 pubmed publisher
  45. Dow J. Insights into the Malpighian tubule from functional genomics. J Exp Biol. 2009;212:435-45 pubmed publisher
  46. Sözen M, Armstrong J, Yang M, Kaiser K, Dow J. Functional domains are specified to single-cell resolution in a Drosophila epithelium. Proc Natl Acad Sci U S A. 1997;94:5207-12 pubmed
    ..Building upon recent advances in Drosophila Malpighian tubule physiology, the present study confirms this tissue as a singular model for integrative physiology...
  47. Wu D, Beyenbach K. The dependence of electrical transport pathways in Malpighian tubules on ATP. J Exp Biol. 2003;206:233-43 pubmed
    ..ATP concentration [ATP](i) and the electrical properties of principal cells was investigated in Malpighian tubules of the yellow fever mosquito, Aedes aegypti. Under control conditions, [ATP](i) was 0...
  48. Coast G, Garside C, Webster S, Schegg K, Schooley D. Mosquito natriuretic peptide identified as a calcitonin-like diuretic hormone in Anopheles gambiae (Giles). J Exp Biol. 2005;208:3281-91 pubmed
    ..peptide Anoga-DH44 and the CT-like peptide Anoga-DH31, which were synthesised and tested for effects on Malpighian tubules from An. gambiae and Ae. aegypti, together with 8-bromo-cyclic AMP...
  49. Wu G, Gao X, Ye G, Li K, Hu C, Cheng J. Ultrastructural alterations in midgut and Malpighian tubules of Boettcherisca peregrina exposure to cadmium and copper. Ecotoxicol Environ Saf. 2009;72:1137-47 pubmed publisher
    ..their at their low concentrations (80microg/g diet) on the morphology and ultrastructure of the midgut and Malpighian tubules of Boettcherisca peregrina larvae were observed by light and transmission electron microscopy...
  50. Beyenbach K, Skaer H, Dow J. The developmental, molecular, and transport biology of Malpighian tubules. Annu Rev Entomol. 2010;55:351-74 pubmed publisher
    Molecular biology is reaching new depths in our understanding of the development and physiology of Malpighian tubules. In Diptera, Malpighian tubules derive from ectodermal cells that evaginate from the primitive hindgut and subsequently ..
  51. Paluzzi J, Naikkhwah W, O Donnell M. Natriuresis and diuretic hormone synergism in R. prolixus upper Malpighian tubules is inhibited by the anti-diuretic hormone, RhoprCAPA-?2. J Insect Physiol. 2012;58:534-42 pubmed publisher
    ..corticotropin releasing factor-related peptide (RhoprDH), and both lead to an increase in fluid secretion by Malpighian tubules (MTs). However, only 5HT activates reabsorption by the lower MTs to recover K(+) and Cl(-)...
  52. Chintapalli V, Terhzaz S, Wang J, Al Bratty M, Watson D, Herzyk P, et al. Functional correlates of positional and gender-specific renal asymmetry in Drosophila. PLoS ONE. 2012;7:e32577 pubmed publisher
    ..Is internal asymmetry functionally significant, or merely an expedient way of packing organs into a cavity?..
  53. Hoch M, Broadie K, Jackle H, Skaer H. Sequential fates in a single cell are established by the neurogenic cascade in the Malpighian tubules of Drosophila. Development. 1994;120:3439-50 pubmed
    ..We demonstrate that both stages in the differentiation of this cell are established by the same sequence of genetic interactions, which have not previously been shown to occur outside the neurogenic ectoderm...
  54. Davies S, Stewart E, Huesmann G, Skaer N, Maddrell S, Tublitz N, et al. Neuropeptide stimulation of the nitric oxide signaling pathway in Drosophila melanogaster Malpighian tubules. Am J Physiol. 1997;273:R823-7 pubmed
    ..We show here by reverse-transcription polymerase chain reaction that Drosophila NOS (dNOS) is expressed in Malpighian tubules. Biochemical assays of NOS activity in whole tubules show that CAP2b significantly stimulates NOS activity...
  55. Lengyel J, Liu X. Posterior gut development in Drosophila: a model system for identifying genes controlling epithelial morphogenesis. Cell Res. 1998;8:273-84 pubmed
    The posterior gut of the Drosophila embryo, consisting of hindgut and Malpighian tubules, provides a simple, well-defined system where it is possible to use a genetic approach to define components essential for epithelial morphogenesis...
  56. Beyenbach K. Regulation of tight junction permeability with switch-like speed. Curr Opin Nephrol Hypertens. 2003;12:543-50 pubmed
    ..The diuretic peptide leucokinin transforms (renal) Malpighian tubules of the yellow fever mosquito from a moderately tight epithelium to a leaky epithelium by increasing the ..
  57. Scott B, Yu M, Lee L, Beyenbach K. Mechanisms of K+ transport across basolateral membranes of principal cells in Malpighian tubules of the yellow fever mosquito, Aedes aegypti. J Exp Biol. 2004;207:1655-63 pubmed
    The mechanisms of K(+) entry from the hemolymph into principal cells of Malpighian tubules were investigated in the yellow fever mosquito, Aedes aegypti...
  58. Ferraro R, Sousa J, Cunha R, Meyer Fernandes J. Characterization of an ecto-phosphatase activity in malpighian tubules of hematophagous bug Rhodnius prolixus. Arch Insect Biochem Physiol. 2004;57:40-9 pubmed
    We have characterized a phosphatase activity present on the external surface of intact Malpighian tubules in Rhodnius prolixus. This phosphatase hydrolyses the substrate p-nitrophenyl phosphate at a rate of 3.38 +/- 0...
  59. Southall T, Terhzaz S, Cabrero P, Chintapalli V, Evans J, Dow J, et al. Novel subcellular locations and functions for secretory pathway Ca2+/Mn2+-ATPases. Physiol Genomics. 2006;26:35-45 pubmed
    ..SPoCk thus contributed to both of the contrasting requirements for Ca2+ in transporting epithelia: to transport or store Ca2+ in bulk without compromising its use as a signal...
  60. Kaneko T, Yano T, Aggarwal K, Lim J, Ueda K, Oshima Y, et al. PGRP-LC and PGRP-LE have essential yet distinct functions in the drosophila immune response to monomeric DAP-type peptidoglycan. Nat Immunol. 2006;7:715-23 pubmed
    ..These data demonstrate that like mammals, drosophila use both extracellular and intracellular receptors, which have conserved signaling mechanisms, for innate immune recognition...
  61. Ruiz Sanchez E, Orchard I, Lange A. Effects of the cyclopeptide mycotoxin destruxin A on the Malpighian tubules of Rhodnius prolixus (Stål). Toxicon. 2010;55:1162-70 pubmed publisher
    ..the effects of destruxin A, a cyclic peptide produced by Metarhizium anispliae, on Rhodnius prolixus Malpighian tubules measuring fluid secretion rate, transepithelial electrical potential (TEP), pH and ion composition of ..
  62. Gautam N, Tapadia M. Ecdysone signaling is required for proper organization and fluid secretion of stellate cells in the Malpighian tubules of Drosophila melanogaster. Int J Dev Biol. 2010;54:635-42 pubmed publisher
    ..Although the larval Malpighian tubules do not seem to be affected by ecdysone during metamorphosis, we show that ecdysone signaling plays an ..
  63. Piermarini P, Hine R, Schepel M, Miyauchi J, Beyenbach K. Role of an apical K,Cl cotransporter in urine formation by renal tubules of the yellow fever mosquito (Aedes aegypti). Am J Physiol Regul Integr Comp Physiol. 2011;301:R1318-37 pubmed publisher
    ..Using RT-PCR on Aedes Malpighian tubules, we identified five alternatively spliced partial cDNAs that encode putative SLC12-like KCCs...
  64. Liu S, Jack J. Regulatory interactions and role in cell type specification of the Malpighian tubules by the cut, Krüppel, and caudal genes of Drosophila. Dev Biol. 1992;150:133-43 pubmed
    ..These three genes are also expressed in the Malpighian tubules before and during differentiation...
  65. Echevarria M, Ramirez Lorca R, Hernández C, Gutierrez A, Mendez Ferrer S, Gonzalez E, et al. Identification of a new water channel (Rp-MIP) in the Malpighian tubules of the insect Rhodnius prolixus. Pflugers Arch. 2001;442:27-34 pubmed
    b>Malpighian tubules (MT) of Rhodnius prolixus transport fluid at very high rates. To identify whether aquaporins (AQPs) are present in the MT of R...
  66. Rheault M, O Donnell M. Analysis of epithelial K(+) transport in Malpighian tubules of Drosophila melanogaster: evidence for spatial and temporal heterogeneity. J Exp Biol. 2001;204:2289-99 pubmed
    Transport of K(+) by the lower, main and distal segments of the Malpighian tubules of Drosophila melanogaster was analyzed using self-referencing K(+)-selective microelectrodes...
  67. Radford J, Davies S, Dow J. Systematic G-protein-coupled receptor analysis in Drosophila melanogaster identifies a leucokinin receptor with novel roles. J Biol Chem. 2002;277:38810-7 pubmed
    ..These tissues also displayed elevated calcium in response to Drosokinin, demonstrating novel roles for leucokinin. A functional genomic approach has thus yielded the first complete characterization of a leucokinin receptor in an insect...
  68. Weng X, Huss M, Wieczorek H, Beyenbach K. The V-type H(+)-ATPase in Malpighian tubules of Aedes aegypti: localization and activity. J Exp Biol. 2003;206:2211-9 pubmed
    ..H(+)-ATPase is thought to provide the driving force for transepithelial electrolyte and fluid secretion in Malpighian tubules. To confirm the presence of this proton pump in Malpighian tubules of the yellow fever mosquito Aedes ..
  69. Dow J, Davies S. The Malpighian tubule: rapid insights from post-genomic biology. J Insect Physiol. 2006;52:365-78 pubmed
    Good osmoregulation is critical to the success of insects, and the Malpighian tubules play a key role in osmoregulation...
  70. Jagge C, Pietrantonio P. Diuretic hormone 44 receptor in Malpighian tubules of the mosquito Aedes aegypti: evidence for transcriptional regulation paralleling urination. Insect Mol Biol. 2008;17:413-26 pubmed publisher
    ..We show that VectorBase gene ID AAEL008292 encodes the DH44 receptor (GPRDIH1) most highly expressed in Malpighian tubules. Sequence analysis and transcript localization indicate that AaegGPRDIH1 is the co-orthologue of the ..
  71. Hofmann T, Chubanov V, Chen X, Dietz A, Gudermann T, Montell C. Drosophila TRPM channel is essential for the control of extracellular magnesium levels. PLoS ONE. 2010;5:e10519 pubmed publisher
    ..we found that the Drosophila TRPM protein was expressed in the fly counterpart of mammalian kidneys, the Malpighian tubules, which function in the removal of electrolytes and toxic components from the hemolymph...
  72. Chen Y, Liu H, Chen H, Tsai F, Chang C, Lee Y, et al. Ethylene glycol induces calcium oxalate crystal deposition in Malpighian tubules: a Drosophila model for nephrolithiasis/urolithiasis. Kidney Int. 2011;80:369-77 pubmed publisher
    ..At different times, the Malpighian tubules, the kidney equivalent of insects, were dissected and a polarized light microscope used to highlight the ..
  73. Faria V, Sucena E. Wolbachia in the Malpighian tubules: evolutionary dead-end or adaptation?. J Exp Zool B Mol Dev Evol. 2013;320:195-9 pubmed publisher
    ..However, throughout the host's life history (Drosophila, for example), some somatic tissues such as the Malpighian tubules (MTs) show large numbers of Wolbachia...
  74. Bertram G, Wessing A. Intracellular pH regulation by the plasma membrane V-ATPase in Malpighian tubules of Drosophila larvae. J Comp Physiol B. 1994;164:238-46 pubmed
    The functional significance of the apical vacuolar-type proton pump (V-ATPase) in Drosophila Malpighian tubules was studied by measuring the intracellular pH (pHi) and luminal pH (pHlu) with double-barrelled pH-microelectrodes in proximal ..
  75. Hege D, Stanewsky R, Hall J, Giebultowicz J. Rhythmic expression of a PER-reporter in the Malpighian tubules of decapitated Drosophila: evidence for a brain-independent circadian clock. J Biol Rhythms. 1997;12:300-8 pubmed
    ..The authors demonstrate that this PER-reporter fusion protein cycles in the Malpighian tubules, showing first cytoplasmic accumulation, which is then followed by translocation of the signal into the ..
  76. Rheault M, O Donnell M. Organic cation transport by Malpighian tubules of Drosophila melanogaster: application of two novel electrophysiological methods. J Exp Biol. 2004;207:2173-84 pubmed
    Transport of the prototypical organic cation tetraethylammonium (TEA) by the Malpighian tubules, ureters and gut of Drosophila melanogaster was studied using two novel electrophysiological techniques...
  77. MacPherson M, Broderick K, Graham S, Day J, Houslay M, Dow J, et al. The dg2 (for) gene confers a renal phenotype in Drosophila by modulation of cGMP-specific phosphodiesterase. J Exp Biol. 2004;207:2769-76 pubmed
  78. Jung A, Denholm B, Skaer H, Affolter M. Renal tubule development in Drosophila: a closer look at the cellular level. J Am Soc Nephrol. 2005;16:322-8 pubmed
    The function of excretion in insects is performed by the Malpighian tubules, a functional equivalent of the vertebrate kidney. Malpighian tubules are long, thin tubes connected to the hindgut...
  79. Cordeiro B, Tibúrcio V, Hallwass M, Paes H, Ribeiro B, Báo S. Structural and ultrastructural alterations of Malpighian tubules of Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae) larvae infected with different Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) recombinant viruses. J Invertebr Pathol. 2008;98:7-19 pubmed publisher
    b>Malpighian tubules constitute the main excretion organ of insects...
  80. Piermarini P, Rouhier M, Schepel M, Kosse C, Beyenbach K. Cloning and functional characterization of inward-rectifying potassium (Kir) channels from Malpighian tubules of the mosquito Aedes aegypti. Insect Biochem Mol Biol. 2013;43:75-90 pubmed publisher
    ..Here, we reveal that three Kir subunits are expressed abundantly in Aedes Malpighian tubules (AeKir1, AeKir2B, and AeKir3); each of their full-length cDNAs was cloned...
  81. Dow J, Maddrell S, Davies S, Skaer N, Kaiser K. A novel role for the nitric oxide-cGMP signaling pathway: the control of epithelial function in Drosophila. Am J Physiol. 1994;266:R1716-9 pubmed
    ..all the elements in the NO pathway are present in, and act to control epithelial fluid secretion by, the Malpighian tubules of an insect, Drosophila melanogaster...
  82. Jack J, Myette G. Mutations that alter the morphology of the malpighian tubules in Drosophila. Dev Genes Evol. 1999;209:546-54 pubmed
    During Drosophila embryogenesis the malpighian tubules evaginate from the hindgut anlage and in a series of morphogenetic events form two pairs of long narrow tubes, each pair emptying into the hindgut through a single ureter...
  83. Broderick K, MacPherson M, Regulski M, Tully T, Dow J, Davies S. Interactions between epithelial nitric oxide signaling and phosphodiesterase activity in Drosophila. Am J Physiol Cell Physiol. 2003;285:C1207-18 pubmed
  84. Torrie L, Radford J, Southall T, Kean L, Dinsmore A, Davies S, et al. Resolution of the insect ouabain paradox. Proc Natl Acad Sci U S A. 2004;101:13689-93 pubmed
    ..These data show that the Na(+),K(+) ATPase, previously thought to be unimportant, may be as vital in insect tissues as in vertebrates, but can be cryptic to conventional pharmacology...
  85. Kaufmann N, Mathai J, Hill W, Dow J, Zeidel M, Brodsky J. Developmental expression and biophysical characterization of a Drosophila melanogaster aquaporin. Am J Physiol Cell Physiol. 2005;289:C397-407 pubmed
    ..This result is consistent with the homology between DRIP and mammalian water-specific AQPs. Together, these data establish Drosophila as a new model system with which to investigate AQP function...
  86. Blumenthal E. Modulation of tyramine signaling by osmolality in an insect secretory epithelium. Am J Physiol Cell Physiol. 2005;289:C1261-7 pubmed
    ..This study concerns the effects of osmolality on the function of the Malpighian tubules (MTs), a major component of the insect excretory system...
  87. Predel R, Neupert S, Garczynski S, Crim J, Brown M, Russell W, et al. Neuropeptidomics of the mosquito Aedes aegypti. J Proteome Res. 2010;9:2006-15 pubmed publisher
    ..Given the high sequence similarity of neuropeptide precursors identified in other medically important mosquitoes, conclusions regarding the peptidome of Ae. aegypti likely are applicable to these mosquitoes...
  88. Chahine S, O Donnell M. Interactions between detoxification mechanisms and excretion in Malpighian tubules of Drosophila melanogaster. J Exp Biol. 2011;214:462-8 pubmed publisher
    ..The results suggest that exposure of Drosophila to toxins evokes a coordinated response by the Malpighian tubules, involving both alterations in detoxification pathways as well as enhanced transport.
  89. Blochlinger K, Jan L, Jan Y. Postembryonic patterns of expression of cut, a locus regulating sensory organ identity in Drosophila. Development. 1993;117:441-50 pubmed
    ..required for the development of a number of other embryonic tissues, such as the central nervous system, the Malpighian tubules and the tracheal system. We here describe the expression of cut in the precursors of adult sensory organs...
  90. Wiehart U, Klein G, Steels P, Nicolson S, Van Kerkhove E. K(+) transport in Malpighian tubules of Tenebrio molitor L: is a K(ATP) channel involved?. J Exp Biol. 2003;206:959-65 pubmed
    The presence of ATP-regulated K(+) (K(ATP)) channels in Tenebrio molitor Malpighian tubules was investigated by examining the effect of glibenclamide on both fluid secretion and basolateral membrane potentials (V(bl))...
  91. Allan A, Du J, Davies S, Dow J. Genome-wide survey of V-ATPase genes in Drosophila reveals a conserved renal phenotype for lethal alleles. Physiol Genomics. 2005;22:128-38 pubmed
    ..These results coincide with the expression view of the gene family, in which 13 of the genes are specialized for epithelial roles, whereas others have spatially or temporally restricted patterns of expression...
  92. Xiang M, Linser P, Price D, Harvey W. Localization of two Na+- or K+-H+ antiporters, AgNHA1 and AgNHA2, in Anopheles gambiae larval Malpighian tubules and the functional expression of AgNHA2 in yeast. J Insect Physiol. 2012;58:570-9 pubmed publisher
    ..Immunolocalization images show AgNHA2 on the apical plasma membrane of stellate cells in Malpighian tubules of An. gambiae larvae and adults...