peptide initiation factors


Summary: Protein factors uniquely required during the initiation phase of protein synthesis in GENETIC TRANSLATION.

Top Publications

  1. Ishfaq M, Maeta K, Maeda S, Natsume T, Ito A, Yoshida M. The role of acetylation in the subcellular localization of an oncogenic isoform of translation factor eIF5A. Biosci Biotechnol Biochem. 2012;76:2165-7 pubmed
    ..HDAC6 and SIRT2 were identified as the enzymes responsible for deacetylating eIF5A2. Analysis using acetylation-deficient mutants indicated that acetylation regulates the subcellular localization of eIF5A2. ..
  2. Luchessi A, Cambiaghi T, Hirabara S, Lambertucci R, Silveira L, Baptista I, et al. Involvement of eukaryotic translation initiation factor 5A (eIF5A) in skeletal muscle stem cell differentiation. J Cell Physiol. 2009;218:480-9 pubmed publisher
    ..These results reveal a new physiological role for eIF5A and contribute to elucidate the molecular mechanisms involved in muscle regeneration. ..
  3. Janic A, Mendizabal L, Llamazares S, Rossell D, Gonzalez C. Ectopic expression of germline genes drives malignant brain tumor growth in Drosophila. Science. 2010;330:1824-7 pubmed publisher
    ..Our results demonstrate that germline traits are necessary for tumor growth in this Drosophila model and suggest that inactivation of germline genes might have tumor-suppressing effects in other species. ..
  4. Nishimura K, Murozumi K, Shirahata A, Park M, Kashiwagi K, Igarashi K. Independent roles of eIF5A and polyamines in cell proliferation. Biochem J. 2005;385:779-85 pubmed
    ..The results show that a decrease in either active eIF5A or polyamines inhibits cell growth, indicating that eIF5A and polyamines are independently involved in cell growth..
  5. Dias C, Cano V, Rangel S, Apponi L, Frigieri M, Muniz J, et al. Structural modeling and mutational analysis of yeast eukaryotic translation initiation factor 5A reveal new critical residues and reinforce its involvement in protein synthesis. FEBS J. 2008;275:1874-88 pubmed publisher
    ..Our data revealed important structural features of eIF5A that are required for its vital role in cell viability and underscored an essential function of eIF5A in the translation step of gene expression. ..
  6. Dinkova T, Keiper B, Korneeva N, Aamodt E, Rhoads R. Translation of a small subset of Caenorhabditis elegans mRNAs is dependent on a specific eukaryotic translation initiation factor 4E isoform. Mol Cell Biol. 2005;25:100-13 pubmed
    ..The functions of these proteins can explain some phenotypes observed in ife-4 knockout mutants. These results indicate that translation of a limited subset of mRNAs is dependent on a specific isoform of eIF4E. ..
  7. Kirino Y, Vourekas A, Sayed N, de Lima Alves F, Thomson T, Lasko P, et al. Arginine methylation of Aubergine mediates Tudor binding and germ plasm localization. RNA. 2010;16:70-8 pubmed publisher
    ..Our study also suggests that the function of the piRNA pathway in PGC specification may be independent of its role in transposon control. ..
  8. Nagao A, Mituyama T, Huang H, Chen D, Siomi M, Siomi H. Biogenesis pathways of piRNAs loaded onto AGO3 in the Drosophila testis. RNA. 2010;16:2503-15 pubmed publisher
    ..These findings suggest that the impacts of armi mutants on the operation of the piRNA pathway are variable in germ cells of fly testes. ..
  9. Patel P, Costa Mattioli M, Schulze K, Bellen H. The Drosophila deoxyhypusine hydroxylase homologue nero and its target eIF5A are required for cell growth and the regulation of autophagy. J Cell Biol. 2009;185:1181-94 pubmed publisher
    ..Our data also indicate that nero and eIF5A are required for cell growth and affect autophagy and protein synthesis. ..

More Information


  1. Lu Z, Liu S, Yao Y, Zhou Y, Zhang S, Dai L, et al. The effect of miR-7 on behavior and global protein expression in glioma cell lines. Electrophoresis. 2011;32:3612-20 pubmed publisher
    ..MiR-7-based gene treatment may be a novel anti-invasion therapeutic strategy for malignant glioma. ..
  2. Zender L, Xue W, Zuber J, Semighini C, Krasnitz A, Ma B, et al. An oncogenomics-based in vivo RNAi screen identifies tumor suppressors in liver cancer. Cell. 2008;135:852-64 pubmed publisher
    ..Our results establish the feasibility of in vivo RNAi screens and illustrate how combining cancer genomics, RNA interference, and mosaic mouse models can facilitate the functional annotation of the cancer genome. ..
  3. Liu Z, Duguay J, Ma F, Wang T, Tshin R, Hopkins M, et al. Modulation of eIF5A1 expression alters xylem abundance in Arabidopsis thaliana. J Exp Bot. 2008;59:939-50 pubmed publisher
    ..These data collectively indicate that eIF5A1 plays a role in xylogenesis. ..
  4. Xu A, Jao D, Chen K. Identification of mRNA that binds to eukaryotic initiation factor 5A by affinity co-purification and differential display. Biochem J. 2004;384:585-90 pubmed
    ..Some, however, encode hypothetical proteins. All the cloned RNAs have the potential to form extensive stem-loop structures. ..
  5. Chiu W, Wagner S, Herrmannová A, Burela L, Zhang F, Saini A, et al. The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons. Mol Cell Biol. 2010;30:4415-34 pubmed publisher
  6. Chattopadhyay M, Park M, Tabor H. Hypusine modification for growth is the major function of spermidine in Saccharomyces cerevisiae polyamine auxotrophs grown in limiting spermidine. Proc Natl Acad Sci U S A. 2008;105:6554-9 pubmed publisher
    ..These data indicate that hypusine modification of eIF5A is a most important function for spermidine in supporting the growth of S. cerevisiae polyamine auxotrophs. ..
  7. Yang G, Xie D, Liu J, Luo J, Li L, Hua W, et al. Expression and amplification of eIF-5A2 in human epithelial ovarian tumors and overexpression of EIF-5A2 is a new independent predictor of outcome in patients with ovarian carcinoma. Gynecol Oncol. 2009;112:314-8 pubmed publisher
  8. Myasnikov A, Simonetti A, Marzi S, Klaholz B. Structure-function insights into prokaryotic and eukaryotic translation initiation. Curr Opin Struct Biol. 2009;19:300-9 pubmed publisher
    ..A comparison of the available prokaryotic and eukaryotic structures shows that--besides significant differences--some key ribosomal features are universally conserved. ..
  9. Gregory R, Chendrimada T, Cooch N, Shiekhattar R. Human RISC couples microRNA biogenesis and posttranscriptional gene silencing. Cell. 2005;123:631-40 pubmed
    ..Importantly, ATP is not required for miRNA processing, RISC assembly, or multiple rounds of target-RNA cleavage. These results define the composition of RISC and demonstrate that miRNA processing and target-RNA cleavage are coupled. ..
  10. Kang K, Kim Y, Wolff E, Park M. Specificity of the deoxyhypusine hydroxylase-eukaryotic translation initiation factor (eIF5A) interaction: identification of amino acid residues of the enzyme required for binding of its substrate, deoxyhypusine-containing eIF5A. J Biol Chem. 2007;282:8300-8 pubmed
    ..These findings support a proposed model of DOHH-eIF5A binding in which the amino group(s) of the deoxyhypusine side chain of the substrate is primarily anchored by gamma-carboxyl groups of Glu57 and Glu208 at the DOHH active site. ..
  11. Luo J, Hua W, Rao H, Liao Y, Kung H, Zeng Y, et al. Overexpression of EIF-5A2 predicts tumor recurrence and progression in pTa/pT1 urothelial carcinoma of the bladder. Cancer Sci. 2009;100:896-902 pubmed publisher
  12. Milon P, Konevega A, Gualerzi C, Rodnina M. Kinetic checkpoint at a late step in translation initiation. Mol Cell. 2008;30:712-20 pubmed publisher
  13. Marshall R, Aitken C, Puglisi J. GTP hydrolysis by IF2 guides progression of the ribosome into elongation. Mol Cell. 2009;35:37-47 pubmed publisher
    ..We observe that rapid GTP hydrolysis by IF2 drives the transition to the elongation-competent conformation, thus committing the ribosome to enter the elongation cycle. ..
  14. Li C, Vagin V, Lee S, Xu J, Ma S, Xi H, et al. Collapse of germline piRNAs in the absence of Argonaute3 reveals somatic piRNAs in flies. Cell. 2009;137:509-21 pubmed publisher
    ..Transposons targeted by this second pathway often reside in the flamenco locus, which is expressed in somatic ovarian follicle cells, suggesting a role for piRNAs beyond the germline. ..
  15. Lee N, Tsang F, Shek F, Mao M, Dai H, Zhang C, et al. Prognostic significance and therapeutic potential of eukaryotic translation initiation factor 5A (eIF5A) in hepatocellular carcinoma. Int J Cancer. 2010;127:968-76 pubmed publisher
  16. Antoun A, Pavlov M, Lovmar M, Ehrenberg M. How initiation factors tune the rate of initiation of protein synthesis in bacteria. EMBO J. 2006;25:2539-50 pubmed
    ..The reasons why IF1 and IF3 are essential in E. coli are discussed in the light of the present observations. ..
  17. Betney R, de Silva E, Krishnan J, Stansfield I. Autoregulatory systems controlling translation factor expression: thermostat-like control of translational accuracy. RNA. 2010;16:655-63 pubmed publisher
  18. Huang Y, Higginson D, Hester L, Park M, Snyder S. Neuronal growth and survival mediated by eIF5A, a polyamine-modified translation initiation factor. Proc Natl Acad Sci U S A. 2007;104:4194-9 pubmed
    ..In brain cultures, inhibition of hypusine formation also inhibits neuronal process extension. ..
  19. Taylor C, Liu Z, Tang T, Zheng Q, Francis S, Wang T, et al. Modulation of eIF5A expression using SNS01 nanoparticles inhibits NF-?B activity and tumor growth in murine models of multiple myeloma. Mol Ther. 2012;20:1305-14 pubmed publisher
    ..05) and RPMI 8226 (59% inhibition; P < 0.05) multiple myeloma xenograft models following systemic administration. These results highlight the potential of using this approach as a new therapeutic strategy for multiple myeloma. ..
  20. Frigieri M, Thompson G, Pandolfi J, Zanelli C, Valentini S. Use of a synthetic lethal screen to identify genes related to TIF51A in Saccharomyces cerevisiae. Genet Mol Res. 2007;6:152-65 pubmed
    ..Future studies will investigate the functional interaction between eIF5A and Ypt1 in order to clarify this involvement of eIF5A with vesicular trafficking. ..
  21. Taylor C, Sun Z, Cliche D, Ming H, Eshaque B, Jin S, et al. Eukaryotic translation initiation factor 5A induces apoptosis in colon cancer cells and associates with the nucleus in response to tumour necrosis factor alpha signalling. Exp Cell Res. 2007;313:437-49 pubmed
    ..These findings collectively indicate that unhypusinated eIF5A may have pro-apoptotic functions and that eIF5A is rapidly translocated to the nucleus following the induction of apoptotic cell death. ..
  22. Cano V, Jeon G, Johansson H, Henderson C, Park J, Valentini S, et al. Mutational analyses of human eIF5A-1--identification of amino acid residues critical for eIF5A activity and hypusine modification. FEBS J. 2008;275:44-58 pubmed
  23. Jasiulionis M, Luchessi A, Moreira A, Souza P, Suenaga A, Correa M, et al. Inhibition of eukaryotic translation initiation factor 5A (eIF5A) hypusination impairs melanoma growth. Cell Biochem Funct. 2007;25:109-14 pubmed
    ..Exposure to GC7 also decreased viability to both cell lines. This study also describes, for the first time, the in vivo antitumour effect of GC7, as indicated by impaired melanoma growth in C57BL/6 mice. ..
  24. Stolboushkina E, Nikonov S, Nikulin A, Bl si U, Manstein D, Fedorov R, et al. Crystal structure of the intact archaeal translation initiation factor 2 demonstrates very high conformational flexibility in the alpha- and beta-subunits. J Mol Biol. 2008;382:680-91 pubmed publisher
  25. Jao D, Chen K. Tandem affinity purification revealed the hypusine-dependent binding of eukaryotic initiation factor 5A to the translating 80S ribosomal complex. J Cell Biochem. 2006;97:583-98 pubmed
  26. Wang S, Liu N, Chen A, Zhao X, Wang J. TRBP homolog interacts with eukaryotic initiation factor 6 (eIF6) in Fenneropenaeus chinensis. J Immunol. 2009;182:5250-8 pubmed publisher
    ..These results indicate that Fc-TRBP and Fc-eIF6 may be components of the RNA-induced silencing complex (RISC), and thereby play a crucial role in the antiviral defense response of shrimp...
  27. Lomakin I, Shirokikh N, Yusupov M, Hellen C, Pestova T. The fidelity of translation initiation: reciprocal activities of eIF1, IF3 and YciH. EMBO J. 2006;25:196-210 pubmed
  28. Leung A, Calabrese J, Sharp P. Quantitative analysis of Argonaute protein reveals microRNA-dependent localization to stress granules. Proc Natl Acad Sci U S A. 2006;103:18125-30 pubmed
    ..Further, miRNAs are required for the Argonaute protein localization to SGs but not PBs. These quantitative kinetic data provide insights into miRNA-mediated repression. ..
  29. He L, Zhao H, Li B, Liu Y, Liu M, Guan X, et al. Overexpression of eIF5A-2 is an adverse prognostic marker of survival in stage I non-small cell lung cancer patients. Int J Cancer. 2011;129:143-50 pubmed publisher
    ..097, p = 0.014). Our findings suggested that overexpression of eIF5A-2 correlates with local invasion of NSCLC, and might serve as an adverse prognostic marker of survival for stage I NSCLC patients. ..
  30. Park M, Nishimura K, Zanelli C, Valentini S. Functional significance of eIF5A and its hypusine modification in eukaryotes. Amino Acids. 2010;38:491-500 pubmed publisher
  31. Pillai R, Bhattacharyya S, Artus C, Zoller T, Cougot N, Basyuk E, et al. Inhibition of translational initiation by Let-7 MicroRNA in human cells. Science. 2005;309:1573-6 pubmed
    ..Repressed mRNAs, Ago proteins, and miRNAs were all found to accumulate in processing bodies. We propose that localization of mRNAs to these structures is a consequence of translational repression. ..
  32. Brennecke J, Malone C, Aravin A, Sachidanandam R, Stark A, Hannon G. An epigenetic role for maternally inherited piRNAs in transposon silencing. Science. 2008;322:1387-92 pubmed publisher
    ..This indicates that maternally deposited piRNAs are important for mounting an effective silencing response and that a lack of maternal piRNA inheritance underlies hybrid dysgenesis. ..
  33. Brennecke J, Aravin A, Stark A, Dus M, Kellis M, Sachidanandam R, et al. Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila. Cell. 2007;128:1089-103 pubmed
    ..Thus, sense piRNAs, formed following cleavage of transposon mRNAs may enhance production of antisense piRNAs, complementary to active elements, by directing cleavage of transcripts from master control loci. ..
  34. Li C, Ohn T, Ivanov P, Tisdale S, Anderson P. eIF5A promotes translation elongation, polysome disassembly and stress granule assembly. PLoS ONE. 2010;5:e9942 pubmed publisher
    ..Our results reveal that hypusine-eIF5A-facilitated translation elongation promotes arsenite-induced polysome disassembly and stress granule assembly in cells subjected to adverse environmental conditions...
  35. Gregio A, Cano V, Avaca J, Valentini S, Zanelli C. eIF5A has a function in the elongation step of translation in yeast. Biochem Biophys Res Commun. 2009;380:785-90 pubmed publisher
    ..Taken together, these results not only reinforce a role for eIF5A in translation but also strongly support a function for eIF5A in the elongation step of protein synthesis. ..
  36. Lorsch J, Dever T. Molecular view of 43 S complex formation and start site selection in eukaryotic translation initiation. J Biol Chem. 2010;285:21203-7 pubmed publisher
    ..A molecular model is emerging in which start codon recognition is linked to dynamic reorganization of factors on the ribosome and structural changes in the ribosome itself. ..
  37. Sun Z, Cheng Z, Taylor C, McConkey B, Thompson J. Apoptosis induction by eIF5A1 involves activation of the intrinsic mitochondrial pathway. J Cell Physiol. 2010;223:798-809 pubmed publisher
    ..These observations collectively indicate that unhypusinated eIF5A1 plays a central role in the regulation of apoptosis. ..
  38. Caraglia M, Park M, Wolff E, Marra M, Abbruzzese A. eIF5A isoforms and cancer: two brothers for two functions?. Amino Acids. 2013;44:103-9 pubmed publisher
    ..eIF5A-1 and/or the eIF5A-2 isoform may serve as a new molecular diagnostic or prognostic marker or as a molecular target for anti-cancer therapy. ..
  39. Pillai R, Artus C, Filipowicz W. Tethering of human Ago proteins to mRNA mimics the miRNA-mediated repression of protein synthesis. RNA. 2004;10:1518-25 pubmed
    ..These findings indicate that a primary function of miRNAs is to guide their associated proteins to the mRNA. ..
  40. Liu J, Valencia Sanchez M, Hannon G, Parker R. MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. Nat Cell Biol. 2005;7:719-23 pubmed
    ..These results provide a link between miRNA function and mammalian P-bodies and suggest that translation repression by RISC delivers mRNAs to P-bodies, either as a cause or as a consequence of inhibiting protein synthesis...
  41. Dyshlovoy S, Naeth I, Venz S, Preukschas M, Sievert H, Jacobsen C, et al. Proteomic profiling of germ cell cancer cells treated with aaptamine, a marine alkaloid with antiproliferative activity. J Proteome Res. 2012;11:2316-30 pubmed publisher
    ..For the first time, we describe changes in protein expression caused by aaptamine, providing valuable information regarding the mode of action of this compound. ..
  42. Rouget C, Papin C, Boureux A, Meunier A, Franco B, Robine N, et al. Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo. Nature. 2010;467:1128-32 pubmed publisher
    ..Because the piRNAs involved in this regulation are produced from transposable elements, this identifies a direct developmental function for transposable elements in the regulation of gene expression. ..
  43. Nishida K, Saito K, Mori T, Kawamura Y, Nagami Okada T, Inagaki S, et al. Gene silencing mechanisms mediated by Aubergine piRNA complexes in Drosophila male gonad. RNA. 2007;13:1911-22 pubmed
    ..These results provide the first biochemical insights into gene silencing mechanisms mediated by Aub and piRNAs in fly testes. ..
  44. Taylor C, Senchyna M, Flanagan J, Joyce E, Cliche D, Boone A, et al. Role of eIF5A in TNF-alpha-mediated apoptosis of lamina cribrosa cells. Invest Ophthalmol Vis Sci. 2004;45:3568-76 pubmed
    ..Thus, eIF5A appears to be a novel proapoptotic protein in the TNF pathway and a possible target for treatment of glaucoma. ..
  45. Zanelli C, Valentini S. Is there a role for eIF5A in translation?. Amino Acids. 2007;33:351-8 pubmed
    ..Finally, recent evidence in favor of reconsidering the role of eIF5A as a translation factor is discussed. Future studies may reveal the specific mechanism by which eIF5A affects protein synthesis. ..
  46. Alone P, Dever T. Direct binding of translation initiation factor eIF2gamma-G domain to its GTPase-activating and GDP-GTP exchange factors eIF5 and eIF2B epsilon. J Biol Chem. 2006;281:12636-44 pubmed
  47. Rehwinkel J, Natalin P, Stark A, Brennecke J, Cohen S, Izaurralde E. Genome-wide analysis of mRNAs regulated by Drosha and Argonaute proteins in Drosophila melanogaster. Mol Cell Biol. 2006;26:2965-75 pubmed
    ..Moreover, AGO1 and AGO2 silence the expression of a common set of mobile genetic elements. Together, these results indicate that the functional overlap between AGO1 and AGO2 in Drosophila is more important than previously thought. ..
  48. Njuguna J, Nassar M, Hoerauf A, Kaiser A. Cloning, expression and functional activity of deoxyhypusine synthase from Plasmodium vivax. BMC Microbiol. 2006;6:91 pubmed
    ..vivax, on the basis of specific enzymatic activity, cross-reactivity with a polyclonal antibody against human DHS, and amino acid identity with DHS homologs from the rodent malaria parasite, P. yoelii, and human P. falciparum strains. ..
  49. Vagin V, Klenov M, Kalmykova A, Stolyarenko A, Kotelnikov R, Gvozdev V. The RNA interference proteins and vasa locus are involved in the silencing of retrotransposons in the female germline of Drosophila melanogaster. RNA Biol. 2004;1:54-8 pubmed
    ..We suggest participation of these proteins in the same silencing pathway. ..
  50. Li A, Li H, Jin B, Ye Q, Zhou T, Yu X, et al. A novel eIF5A complex functions as a regulator of p53 and p53-dependent apoptosis. J Biol Chem. 2004;279:49251-8 pubmed
    ..Therefore, eIF5A seems to be a previously unrecognized regulator of p53 that may define a new pathway for p53-dependent apoptosis, and syntenin might regulate p53 by balancing the regulation of eIF5A signaling to p53 for apoptosis. ..
  51. Gunawardane L, Saito K, Nishida K, Miyoshi K, Kawamura Y, Nagami T, et al. A slicer-mediated mechanism for repeat-associated siRNA 5' end formation in Drosophila. Science. 2007;315:1587-90 pubmed
    ..These data support a model in which formation of a 5' terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with the Slicer activity of PIWI. ..
  52. Savitsky M, Kwon D, Georgiev P, Kalmykova A, Gvozdev V. Telomere elongation is under the control of the RNAi-based mechanism in the Drosophila germline. Genes Dev. 2006;20:345-54 pubmed
    ..Double-stranded RNA (dsRNA)-mediated control of telomeric retroelement transposition may occur at premeiotic stages, resulting in the maintenance of appropriate telomere length in gamete precursors. ..
  53. Vu V, Emerson J, Martinho M, Kim Y, Munck E, Park M, et al. Human deoxyhypusine hydroxylase, an enzyme involved in regulating cell growth, activates O2 with a nonheme diiron center. Proc Natl Acad Sci U S A. 2009;106:14814-9 pubmed publisher
    ..Notably, hDOHH is the only example thus far of a human hydroxylase with such a diiron active site. ..
  54. Nishimura K, Lee S, Park J, Park M. Essential role of eIF5A-1 and deoxyhypusine synthase in mouse embryonic development. Amino Acids. 2012;42:703-10 pubmed publisher
  55. Zhu W, Cai M, Tong Z, Dong S, Mai S, Liao Y, et al. Overexpression of EIF5A2 promotes colorectal carcinoma cell aggressiveness by upregulating MTA1 through C-myc to induce epithelial-mesenchymaltransition. Gut. 2012;61:562-75 pubmed publisher
    ..The data suggest that EIF5A2 plays an important oncogenic role in CRC aggressiveness by the upregulation of MTA1 to induce EMT, and EIF5A2 could be employed as a novel prognostic marker and/or effective therapeutic target for CRC. ..
  56. Balabanov S, Gontarewicz A, Ziegler P, Hartmann U, Kammer W, Copland M, et al. Hypusination of eukaryotic initiation factor 5A (eIF5A): a novel therapeutic target in BCR-ABL-positive leukemias identified by a proteomics approach. Blood. 2007;109:1701-11 pubmed
    ..In conclusion, through a comparative proteomics approach and further functional analysis, we identified the inhibition of eIF5A hypusination as a promising new approach for combination therapy in BCR-ABL-positive leukemias. ..
  57. Yatime L, Mechulam Y, Blanquet S, Schmitt E. Structural switch of the gamma subunit in an archaeal aIF2 alpha gamma heterodimer. Structure. 2006;14:119-28 pubmed
    ..Because the alpha subunit markedly reinforces the affinity of tRNA for the gamma subunit, a contribution of the alpha subunit to the switch movements observed in the gamma structure is considered. ..
  58. Chen Y, Pane A, Schupbach T. Cutoff and aubergine mutations result in retrotransposon upregulation and checkpoint activation in Drosophila. Curr Biol. 2007;17:637-42 pubmed
  59. Feng H, Chen Q, Feng J, Zhang J, Yang X, Zuo J. Functional characterization of the Arabidopsis eukaryotic translation initiation factor 5A-2 that plays a crucial role in plant growth and development by regulating cell division, cell growth, and cell death. Plant Physiol. 2007;144:1531-45 pubmed
    ..We propose that FBR12/eIF-5A-2 is fundamental for plant growth and development by regulating cell division, cell growth, and cell death. ..
  60. Pane A, Wehr K, Schupbach T. zucchini and squash encode two putative nucleases required for rasiRNA production in the Drosophila germline. Dev Cell. 2007;12:851-62 pubmed
    ..We show that these defects are due to the inability of zuc and squ mutants to produce repeat-associated small interfering RNAs. ..
  61. Zanelli C, Valentini S. Pkc1 acts through Zds1 and Gic1 to suppress growth and cell polarity defects of a yeast eIF5A mutant. Genetics. 2005;171:1571-81 pubmed
    ..Taken together, these data strongly support the correlated involvement of Pkc1 and eIF5A in establishing actin polarity, which is essential for bud formation and G1/S transition in S. cerevisiae. ..
  62. Park J, Aravind L, Wolff E, Kaevel J, Kim Y, Park M. Molecular cloning, expression, and structural prediction of deoxyhypusine hydroxylase: a HEAT-repeat-containing metalloenzyme. Proc Natl Acad Sci U S A. 2006;103:51-6 pubmed
    ..However, metal coordination sites composed of four strictly conserved histidine-glutamate sequences were identified, suggesting that DOHH enzymes have convergently evolved an iron-dependent hydroxylation mechanism. ..
  63. Tang D, Dong S, Ma N, Xie D, Chen L, Fu L, et al. Overexpression of eukaryotic initiation factor 5A2 enhances cell motility and promotes tumor metastasis in hepatocellular carcinoma. Hepatology. 2010;51:1255-63 pubmed publisher
    ..EIF5A2 plays an important role in HCC invasion and metastasis by inducing EMT, as well as stimulating cytoskeleton rearrangement through activation of RhoA and Rac1. ..
  64. Pisarev A, Hellen C, Pestova T. Recycling of eukaryotic posttermination ribosomal complexes. Cell. 2007;131:286-99 pubmed
    ..Its activity is enhanced by eIFs 3j, 1, and 1A. eIF1 also mediates release of P site tRNA, whereas eIF3j ensures subsequent dissociation of mRNA. ..
  65. Hanawa Suetsugu K, Sekine S, Sakai H, Hori Takemoto C, Terada T, Unzai S, et al. Crystal structure of elongation factor P from Thermus thermophilus HB8. Proc Natl Acad Sci U S A. 2004;101:9595-600 pubmed publisher
    ..Therefore, EF-P mimics the tRNA shape but uses domain topologies different from those of the known tRNA-mimicry translation factors. Domain I of EF-P has a conserved positive charge at its tip, like the eIF-5A N domain...
  66. Ma F, Liu Z, Wang T, Hopkins M, Peterson C, Thompson J. Arabidopsis eIF5A3 influences growth and the response to osmotic and nutrient stress. Plant Cell Environ. 2010;33:1682-96 pubmed publisher
    ..Thus, AteIF5A3 appears to be involved in supporting growth and to play a regulatory role in the response of plants to sub-lethal osmotic and nutrient stress. ..
  67. Hauber J. Revisiting an old acquaintance: role for eIF5A in diabetes. J Clin Invest. 2010;120:1806-8 pubmed publisher
    ..I believe these data provide new and important insights into the regulatory pathways that contribute to the development of diabetes and deepen our understanding of the function of the, so far, rather enigmatic cellular protein eIF5A. ..
  68. Sen G, Blau H. Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies. Nat Cell Biol. 2005;7:633-6 pubmed
    ..In addition, Argonaute 1, another Argonaute family member, is concentrated in cytoplasmic bodies. These results provide new insight into the mechanism of RNAi function. ..
  69. Shirokikh N, Spirin A. Poly(A) leader of eukaryotic mRNA bypasses the dependence of translation on initiation factors. Proc Natl Acad Sci U S A. 2008;105:10738-43 pubmed publisher
  70. Saini P, Eyler D, Green R, Dever T. Hypusine-containing protein eIF5A promotes translation elongation. Nature. 2009;459:118-21 pubmed publisher
    ..Because eIF5A is a structural homologue of the bacterial protein EF-P, we propose that eIF5A/EF-P is a universally conserved translation elongation factor. ..
  71. Pisarev A, Shirokikh N, Hellen C. Translation initiation by factor-independent binding of eukaryotic ribosomes to internal ribosomal entry sites. C R Biol. 2005;328:589-605 pubmed
  72. Chatterjee I, Gross S, Kinzy T, Chen K. Rapid depletion of mutant eukaryotic initiation factor 5A at restrictive temperature reveals connections to actin cytoskeleton and cell cycle progression. Mol Genet Genomics. 2006;275:264-76 pubmed
    ..Sorbitol, an osmotic stabilizer that complement defects in PKC/WSC pathways, stabilizes the mutant eIF5A and suppresses all the observed temperature-sensitive phenotypes. ..
  73. Nielsen K, VALASEK L, Sykes C, Jivotovskaya A, Hinnebusch A. Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast. Mol Cell Biol. 2006;26:2984-98 pubmed
    ..Thus, the PRT1 RNP1 motif coordinates the functions of HCR1 and TIF32 in 40S binding of eIF3 and is needed for optimal preinitiation complex assembly and AUG recognition in vivo. ..
  74. Yatime L, Mechulam Y, Blanquet S, Schmitt E. Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states. Proc Natl Acad Sci U S A. 2007;104:18445-50 pubmed publisher
    ..The nucleotide state of aIF2 shown here is indicative of a similar mechanism in archaea. Finally, we consider the possibility that release of Pi takes place after e/aIF2gamma has been informed of e/aIF1 dissociation by e/aIF2beta...
  75. Zanelli C, Maragno A, Gregio A, Komili S, Pandolfi J, Mestriner C, et al. eIF5A binds to translational machinery components and affects translation in yeast. Biochem Biophys Res Commun. 2006;348:1358-66 pubmed
    ..Our results re-establish a function for eIF5A in translation and suggest a role for this factor in translation elongation instead of translation initiation. ..
  76. Schrader R, Young C, Kozian D, Hoffmann R, Lottspeich F. Temperature-sensitive eIF5A mutant accumulates transcripts targeted to the nonsense-mediated decay pathway. J Biol Chem. 2006;281:35336-46 pubmed
    ..Our data suggest that eIF5A mediates important cellular processes like cell viability and senescence through its effects on the stability of certain mRNAs. ..
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    ..The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage. ..
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    ..Our study indicates that, unlike other RNase III type proteins, human Dicer may employ two different dsRBD-containing proteins that facilitate RISC assembly. ..
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    ..This binding may also influence the half-lives of mRNAs or their sequestration. Based on these data, there is a considerable therapeutic interest in eIF5A as a selective target for drug development through inhibition of hypusination. ..
  81. Klattenhoff C, Bratu D, McGinnis Schultz N, Koppetsch B, Cook H, Theurkauf W. Drosophila rasiRNA pathway mutations disrupt embryonic axis specification through activation of an ATR/Chk2 DNA damage response. Dev Cell. 2007;12:45-55 pubmed
    ..We conclude that rasiRNA-based gene silencing is not required for axis specification, and that the critical developmental function for this pathway is to suppress DNA damage signaling in the germline. ..
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    ..Taken together these data reveal a link between eIF6 and Wnt signaling, perhaps at the level of ribosome recycling on beta-catenin mRNA. ..
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    ..Examples are described. Some mistaken ideas about regulation of translation that have found their way into textbooks are pointed out and corrected. ..
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    ..The results indicate that AteIF5A-2 is a key element of the signal transduction pathway resulting in plant programmed cell death. ..
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    ..Our findings underscore the significance of sDMA modification of Piwi proteins in the germline and suggest an interacting pathway of genes that are required for piRNA function and PGC specification. ..