signal recognition particle

Summary

Summary: A cytosolic ribonucleoprotein complex that acts to induce elongation arrest of nascent presecretory and membrane proteins until the ribosome becomes associated with the rough endoplasmic reticulum. It consists of a 7S RNA and at least six polypeptide subunits (relative molecular masses 9, 14, 19, 54, 68, and 72K).

Top Publications

  1. Eddy S. A memory-efficient dynamic programming algorithm for optimal alignment of a sequence to an RNA secondary structure. BMC Bioinformatics. 2002;3:18 pubmed
    ..The new algorithm has an O(N2 log N) memory complexity, at the expense of a small constant factor in time. Optimal ribosomal RNA structural alignments that previously required up to 150 GB of memory now require less than 270 MB. ..
  2. Nagai K, Oubridge C, Kuglstatter A, Menichelli E, Isel C, Jovine L. Structure, function and evolution of the signal recognition particle. EMBO J. 2003;22:3479-85 pubmed
    The signal recognition particle (SRP) is a ribonucleoprotein particle essential for the targeting of signal peptide-bearing proteins to the prokaryotic plasma membrane or the eukaryotic endoplasmic reticulum membrane for secretion or ..
  3. Shen K, Arslan S, Akopian D, Ha T, Shan S. Activated GTPase movement on an RNA scaffold drives co-translational protein targeting. Nature. 2012;492:271-5 pubmed publisher
    ..localization of these proteins is mediated by a universally conserved protein-targeting machinery, the signal recognition particle (SRP), which recognizes ribosomes carrying signal sequences and, through interactions with the SRP ..
  4. Sommerville J, Brumwell C, Politz J, Pederson T. Signal recognition particle assembly in relation to the function of amplified nucleoli of Xenopus oocytes. J Cell Sci. 2005;118:1299-307 pubmed
    The signal recognition particle (SRP) is a ribonucleoprotein machine that controls the translation and intracellular sorting of membrane and secreted proteins. The SRP contains a core RNA subunit with which six proteins are assembled...
  5. Grudnik P, Bange G, Sinning I. Protein targeting by the signal recognition particle. Biol Chem. 2009;390:775-82 pubmed publisher
    Protein targeting by the signal recognition particle (SRP) is universally conserved and starts with the recognition of a signal sequence in the context of a translating ribosome...
  6. Didierlaurent L, Racine P, Houzet L, Chamontin C, Berkhout B, Mougel M. Role of HIV-1 RNA and protein determinants for the selective packaging of spliced and unspliced viral RNA and host U6 and 7SL RNA in virus particles. Nucleic Acids Res. 2011;39:8915-27 pubmed publisher
    ..Thus, GagNC is a major contributor to the packaging selectivity. Altogether our results provide new molecular insight on how HIV selects distinct RNA species for incorporation into particles. ..
  7. Jermy A, Willer M, Davis E, Wilkinson B, Stirling C. The Brl domain in Sec63p is required for assembly of functional endoplasmic reticulum translocons. J Biol Chem. 2006;281:7899-906 pubmed
    ..The signal recognition particle-dependent pathway targets nascent precursors co-translationally, whereas the Sec62p-dependent pathway ..
  8. Zhang X, Kung S, Shan S. Demonstration of a multistep mechanism for assembly of the SRP x SRP receptor complex: implications for the catalytic role of SRP RNA. J Mol Biol. 2008;381:581-93 pubmed publisher
    Two GTPases in the signal recognition particle (SRP) and its receptor (SR) control the delivery of newly synthesized proteins to the endoplasmic reticulum or plasma membrane. During the protein targeting reaction, the 4...
  9. Benveniste O, Drouot L, Jouen F, Charuel J, Bloch Queyrat C, Behin A, et al. Correlation of anti-signal recognition particle autoantibody levels with creatine kinase activity in patients with necrotizing myopathy. Arthritis Rheum. 2011;63:1961-71 pubmed publisher
    Anti-signal recognition particle (anti-SRP) autoantibodies are associated with severe acquired necrotizing myopathies. The role of these autoantibodies remains elusive, and the evolution of anti-SRP levels over time is unknown...

More Information

Publications106 found, 100 shown here

  1. Tian H, Beckwith J. Genetic screen yields mutations in genes encoding all known components of the Escherichia coli signal recognition particle pathway. J Bacteriol. 2002;184:111-8 pubmed
    ..The screen yielded mutations in each of the known genes encoding components of the E. coli signal recognition particle pathway: ffh, ffs, and ftsY, which encode Ffh, 4.5S RNA, and FtsY, respectively...
  2. Nilsson R, van Wijk K. Transient interaction of cpSRP54 with elongating nascent chains of the chloroplast-encoded D1 protein; 'cpSRP54 caught in the act'. FEBS Lett. 2002;524:127-33 pubmed
    The signal recognition particle (SRP) in bacteria and endoplasmic reticulum is involved in co-translational targeting. Plastids contain cpSRP54 and cpSRP43, unique to plants, but lack a SRP RNA molecule...
  3. Hainzl T, Huang S, Sauer Eriksson A. Structural insights into SRP RNA: an induced fit mechanism for SRP assembly. RNA. 2005;11:1043-50 pubmed
    ..The signal recognition particle (SRP) is a multiprotein-RNA complex responsible for the cotranslational targeting of proteins to ..
  4. Lam V, Akopian D, Rome M, Henningsen D, Shan S. Lipid activation of the signal recognition particle receptor provides spatial coordination of protein targeting. J Cell Biol. 2010;190:623-35 pubmed publisher
    The signal recognition particle (SRP) and SRP receptor comprise the major cellular machinery that mediates the cotranslational targeting of proteins to cellular membranes...
  5. Wild K, Rosendal K, Sinning I. A structural step into the SRP cycle. Mol Microbiol. 2004;53:357-63 pubmed
    ..targeting of secretory and membrane proteins to the translocation machinery is mediated by the signal recognition particle (SRP) and its membrane-bound receptor (SR) in all three domains of life...
  6. Buskiewicz I, Peske F, Wieden H, Gryczynski I, Rodnina M, Wintermeyer W. Conformations of the signal recognition particle protein Ffh from Escherichia coli as determined by FRET. J Mol Biol. 2005;351:417-30 pubmed
    The signal recognition particle (SRP) initiates the co-translational targeting of proteins to the plasma membrane in bacteria by binding to the N-terminal signal sequence emerging from the translating ribosome...
  7. Iakhiaeva E, Hinck C, Hinck A, Zwieb C. Characterization of the SRP68/72 interface of human signal recognition particle by systematic site-directed mutagenesis. Protein Sci. 2009;18:2183-95 pubmed publisher
    The signal recognition particle (SRP) is a ribonucleoprotein complex which is crucial for the delivery of proteins to cellular membranes...
  8. Egea P, Stroud R, Walter P. Targeting proteins to membranes: structure of the signal recognition particle. Curr Opin Struct Biol. 2005;15:213-20 pubmed
    ..plasma membrane or eukaryotic endoplasmic reticulum is mediated by a ribonucleoprotein complex, the signal recognition particle (SRP), and its membrane-associated receptor (SR)...
  9. Ullers R, Houben E, Brunner J, Oudega B, Harms N, Luirink J. Sequence-specific interactions of nascent Escherichia coli polypeptides with trigger factor and signal recognition particle. J Biol Chem. 2006;281:13999-4005 pubmed
    ..The signal recognition particle (SRP) effectively competes the contact with TF when a signal anchor (SA) sequence of a nascent inner ..
  10. Gawronski Salerno J, Freymann D. Structure of the GMPPNP-stabilized NG domain complex of the SRP GTPases Ffh and FtsY. J Struct Biol. 2007;158:122-8 pubmed
    Ffh and FtsY are GTPase components of the signal recognition particle co-translational targeting complex that assemble during the SRP cycle to form a GTP-dependent and pseudo twofold symmetric heterodimer...
  11. Menichelli E, Isel C, Oubridge C, Nagai K. Protein-induced conformational changes of RNA during the assembly of human signal recognition particle. J Mol Biol. 2007;367:187-203 pubmed
    The human signal recognition particle (SRP) is a large RNA-protein complex that targets secretory and membrane proteins to the endoplasmic reticulum membrane...
  12. Leipe D, Wolf Y, Koonin E, Aravind L. Classification and evolution of P-loop GTPases and related ATPases. J Mol Biol. 2002;317:41-72 pubmed
    ..The second class, designated SIMIBI (after signal recognition particle, MinD, and BioD), consists of signal recognition particle (SRP) GTPases, the assemblage of MinD-like ..
  13. Rose R, Pohlschr der M. In vivo analysis of an essential archaeal signal recognition particle in its native host. J Bacteriol. 2002;184:3260-7 pubmed
    The evolutionarily conserved signal recognition particle (SRP) plays an integral role in Sec-mediated cotranslational protein translocation and membrane protein insertion, as it has been shown to target nascent secretory and membrane ..
  14. Rosendal K, Wild K, Montoya G, Sinning I. Crystal structure of the complete core of archaeal signal recognition particle and implications for interdomain communication. Proc Natl Acad Sci U S A. 2003;100:14701-6 pubmed publisher
    Targeting of secretory and membrane proteins by the signal recognition particle (SRP) is evolutionarily conserved, and the multidomain protein SRP54 acts as the key player in SRP-mediated protein transport...
  15. Strebel K, Khan M. APOBEC3G encapsidation into HIV-1 virions: which RNA is it?. Retrovirology. 2008;5:55 pubmed publisher
    ..There is increasing evidence that RNA binding of APOBEC3G is important for packaging into viral particles. However, there is no consensus yet on the type of RNA involved. ..
  16. del Alamo M, Hogan D, Pechmann S, Albanèse V, Brown P, Frydman J. Defining the specificity of cotranslationally acting chaperones by systematic analysis of mRNAs associated with ribosome-nascent chain complexes. PLoS Biol. 2011;9:e1001100 pubmed publisher
    ..We here focused on two Saccharomyces cerevisiae chaperones: the Signal Recognition Particle (SRP), which acts cotranslationally to target proteins to the ER, and the Nascent chain Associated ..
  17. Focia P, Alam H, Lu T, Ramirez U, Freymann D. Novel protein and Mg2+ configurations in the Mg2+GDP complex of the SRP GTPase ffh. Proteins. 2004;54:222-30 pubmed
    Ffh is the signal sequence recognition and targeting subunit of the prokaryotic signal recognition particle (SRP)...
  18. Shan S, Chandrasekar S, Walter P. Conformational changes in the GTPase modules of the signal reception particle and its receptor drive initiation of protein translocation. J Cell Biol. 2007;178:611-20 pubmed
    During cotranslational protein targeting, two guanosine triphosphatase (GTPase) in the signal recognition particle (SRP) and its receptor (SR) form a unique complex in which hydrolyses of both guanosine triphosphates (GTP) are activated ..
  19. Shao S, Hegde R. Membrane protein insertion at the endoplasmic reticulum. Annu Rev Cell Dev Biol. 2011;27:25-56 pubmed publisher
    ..The classical cotranslational pathway, utilized by most membrane proteins, involves targeting by the signal recognition particle followed by insertion via the Sec61 translocon...
  20. Rabu C, Schmid V, Schwappach B, High S. Biogenesis of tail-anchored proteins: the beginning for the end?. J Cell Sci. 2009;122:3605-12 pubmed publisher
  21. Zhang D, Shan S. Translation elongation regulates substrate selection by the signal recognition particle. J Biol Chem. 2012;287:7652-60 pubmed publisher
    The signal recognition particle (SRP) is a universally conserved cellular machinery responsible for delivering membrane and secretory proteins to the proper cellular destination...
  22. Fröderberg L, Houben E, Samuelson J, Chen M, Park S, Phillips G, et al. Versatility of inner membrane protein biogenesis in Escherichia coli. Mol Microbiol. 2003;47:1015-27 pubmed
    ..of a set of model IMPs covering most IMP structures possible has been studied in a variety of signal recognition particle (SRP), Sec and YidC mutant strains...
  23. Andersen E, Rosenblad M, Larsen N, Westergaard J, Burks J, Wower I, et al. The tmRDB and SRPDB resources. Nucleic Acids Res. 2006;34:D163-8 pubmed publisher
    ..kvl.dk/). The signal recognition particle database (SRPDB) at http://psyche.uthct.edu/dbs/SRPDB/SRPDB.html is mirrored at http://srpdb.kvl...
  24. Jaru Ampornpan P, Chandrasekar S, Shan S. Efficient interaction between two GTPases allows the chloroplast SRP pathway to bypass the requirement for an SRP RNA. Mol Biol Cell. 2007;18:2636-45 pubmed
    Cotranslational protein targeting to membranes is regulated by two GTPases in the signal recognition particle (SRP) and the SRP receptor; association between the two GTPases is slow and is accelerated 400-fold by the SRP RNA...
  25. Onafuwa Nuga A, King S, Telesnitsky A. Nonrandom packaging of host RNAs in moloney murine leukemia virus. J Virol. 2005;79:13528-37 pubmed
    ..These data demonstrate that certain host RNAs are nearly as enriched in virions as genomic RNA and suggest that Psi- mRNAs and some other host RNAs may be specifically excluded from assembly sites...
  26. Pyhtila B, Zheng T, Lager P, Keene J, Reedy M, Nicchitta C. Signal sequence- and translation-independent mRNA localization to the endoplasmic reticulum. RNA. 2008;14:445-53 pubmed publisher
    ..to the endoplasmic reticulum (ER), in contrast, occurs via a co-translational, signal sequence/signal recognition particle (SRP)-dependent mechanism...
  27. Moll R. The archaeal signal recognition particle: steps toward membrane binding. J Bioenerg Biomembr. 2004;36:47-53 pubmed
    ..Sci. U.S.A. 74, 5088-5090) raises questions concerning the structure and composition of the archaeal signal recognition particle as well as the functions that route nascent prepoly peptide chains to the membrane...
  28. Gerst J. Message on the web: mRNA and ER co-trafficking. Trends Cell Biol. 2008;18:68-76 pubmed publisher
    ..e. during cell division in yeast), or enable confined spatial responses to environmental stimuli (i.e. during synaptic remodeling or in cases of neuronal injury). ..
  29. Falk S, Ravaud S, Koch J, Sinning I. The C terminus of the Alb3 membrane insertase recruits cpSRP43 to the thylakoid membrane. J Biol Chem. 2010;285:5954-62 pubmed publisher
    ..Here we describe the molecular mechanisms underlying the interaction of Alb3 with the chloroplast signal recognition particle (cpSRP)...
  30. Rosenblad M, Samuelsson T. Identification of chloroplast signal recognition particle RNA genes. Plant Cell Physiol. 2004;45:1633-9 pubmed
    The signal recognition particle (SRP) is a ribonucleoprotein complex responsible for targeting proteins to the ER membrane in eukaryotes, the plasma membrane in bacteria and the thylakoid membrane in chloroplasts...
  31. Dalley J, Selkirk A, Pool M. Access to ribosomal protein Rpl25p by the signal recognition particle is required for efficient cotranslational translocation. Mol Biol Cell. 2008;19:2876-84 pubmed publisher
    ..to the endoplasmic reticulum (ER) occurs cotranslationally necessitating the interaction of the signal recognition particle (SRP) and the translocon with the ribosome...
  32. Moore M, Goforth R, Mori H, Henry R. Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids: substrate not required. J Cell Biol. 2003;162:1245-54 pubmed
    Integration of thylakoid proteins by the chloroplast signal recognition particle (cpSRP) posttranslational transport pathway requires the cpSRP, an SRP receptor homologue (cpFtsY), and the membrane protein ALB3...
  33. Sivaraja V, Kumar T, Leena P, Chang A, Vidya C, Goforth R, et al. Three-dimensional solution structures of the chromodomains of cpSRP43. J Biol Chem. 2005;280:41465-71 pubmed
    Chloroplasts contain a unique signal recognition particle (cpSRP). Unlike the cytoplasmic forms, the cpSRP lacks RNA but contains a conserved 54-kDa GTPase and a novel 43-kDa subunit (cpSRP43)...
  34. Rosch J, Vega L, Beyer J, Lin A, Caparon M. The signal recognition particle pathway is required for virulence in Streptococcus pyogenes. Infect Immun. 2008;76:2612-9 pubmed publisher
    The signal recognition particle (SRP) pathway is a universally conserved pathway for targeting polypeptides for secretion via the cotranslational pathway...
  35. Rosenblad M, Larsen N, Samuelsson T, Zwieb C. Kinship in the SRP RNA family. RNA Biol. 2009;6:508-16 pubmed
    The signal recognition particle (SRP) is a ribonucleoprotein complex which participates in the targeting of protein to cellular membranes...
  36. Valent Q, de Gier J, von Heijne G, Kendall D, Ten Hagen Jongman C, Oudega B, et al. Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factor. Mol Microbiol. 1997;25:53-64 pubmed
    The Escherichia coli signal recognition particle (SRP) and trigger factor are cytoplasmic factors that interact with short nascent polypeptides of presecretory and membrane proteins produced in a heterologous in vitro translation system...
  37. Rinke Appel J, Osswald M, von Knoblauch K, Mueller F, Brimacombe R, Sergiev P, et al. Crosslinking of 4.5S RNA to the Escherichia coli ribosome in the presence or absence of the protein Ffh. RNA. 2002;8:612-25 pubmed
    ..5S molecule, and this was docked onto the ribosome using the crosslinking data. The results are discussed in terms of multiple functions and binding sites of the 4.5S RNA. ..
  38. Rospert S, Dubaquie Y, Gautschi M. Nascent-polypeptide-associated complex. Cell Mol Life Sci. 2002;59:1632-9 pubmed
    ..This review attempts to summarize the data from different fields and to discuss the partly controversial results in a common context. ..
  39. Funke S, Knechten T, Ollesch J, Schünemann D. A unique sequence motif in the 54-kDa subunit of the chloroplast signal recognition particle mediates binding to the 43-kDa subunit. J Biol Chem. 2005;280:8912-7 pubmed
    Chloroplasts contain a novel type of signal recognition particle (cpSRP) that consists of two proteins, cpSRP54 and cpSRP43...
  40. Iakhiaeva E, Bhuiyan S, Yin J, Zwieb C. Protein SRP68 of human signal recognition particle: identification of the RNA and SRP72 binding domains. Protein Sci. 2006;15:1290-302 pubmed
    The signal recognition particle (SRP) plays an important role in the delivery of secretory proteins to cellular membranes...
  41. Bornemann T, Jöckel J, Rodnina M, Wintermeyer W. Signal sequence-independent membrane targeting of ribosomes containing short nascent peptides within the exit tunnel. Nat Struct Mol Biol. 2008;15:494-9 pubmed publisher
    ..membrane proteins in Escherichia coli are targeted to the translocon in the plasma membrane by the signal recognition particle (SRP) and the SRP receptor, FtsY...
  42. Janda C, Li J, Oubridge C, Hernandez H, Robinson C, Nagai K. Recognition of a signal peptide by the signal recognition particle. Nature. 2010;465:507-10 pubmed publisher
    ..and membrane proteins usually contain an amino-terminal signal peptide, which is recognized by the signal recognition particle (SRP) when nascent polypeptide chains emerge from the ribosome...
  43. Gu S, Peske F, Wieden H, Rodnina M, Wintermeyer W. The signal recognition particle binds to protein L23 at the peptide exit of the Escherichia coli ribosome. RNA. 2003;9:566-73 pubmed
    The signal recognition particle (SRP) from Escherichia coli, composed of Ffh protein and 4.5S RNA, mediates membrane targeting of translating ribosomes displaying a signal or signal-anchor sequence...
  44. Huck L, Scherrer A, Terzi L, Johnson A, Bernstein H, Cusack S, et al. Conserved tertiary base pairing ensures proper RNA folding and efficient assembly of the signal recognition particle Alu domain. Nucleic Acids Res. 2004;32:4915-24 pubmed
    ..the role of conserved base pairs formed between two distant loops in the Alu portion of the mammalian signal recognition particle RNA (SRP RNA) in SRP assembly and functions...
  45. Zwieb C, Eichler J. Getting on target: the archaeal signal recognition particle. Archaea. 2002;1:27-34 pubmed
    ..In Eukarya and Bacteria, this is achieved through the interaction of the signal recognition particle (SRP) with the nascent polypeptide chain...
  46. Huber D, Boyd D, Xia Y, Olma M, Gerstein M, Beckwith J. Use of thioredoxin as a reporter to identify a subset of Escherichia coli signal sequences that promote signal recognition particle-dependent translocation. J Bacteriol. 2005;187:2983-91 pubmed
    ..efficient, cotranslational translocation of the cytoplasmic protein thioredoxin-1 via the bacterial signal recognition particle (SRP) pathway...
  47. Wang T, Tian C, Zhang W, Luo K, Sarkis P, Yu L, et al. 7SL RNA mediates virion packaging of the antiviral cytidine deaminase APOBEC3G. J Virol. 2007;81:13112-24 pubmed
    ..This selective interaction of A3G with certain Pol III-derived RNAs raises the question of whether A3G and its cofactors may have as-yet-unidentified cellular functions. ..
  48. Iakhiaeva E, Wower J, Wower I, Zwieb C. The 5e motif of eukaryotic signal recognition particle RNA contains a conserved adenosine for the binding of SRP72. RNA. 2008;14:1143-53 pubmed publisher
    The signal recognition particle (SRP) plays a pivotal role in transporting proteins to cell membranes. In higher eukaryotes, SRP consists of an RNA molecule and six proteins...
  49. Sijbrandi R, Urbanus M, ten Hagen Jongman C, Bernstein H, Oudega B, Otto B, et al. Signal recognition particle (SRP)-mediated targeting and Sec-dependent translocation of an extracellular Escherichia coli protein. J Biol Chem. 2003;278:4654-9 pubmed publisher
    ..Here, we present evidence that Hbp interacts with the signal recognition particle (SRP) and the Sec-translocon early during biogenesis...
  50. Bowers C, Lau F, Silhavy T. Secretion of LamB-LacZ by the signal recognition particle pathway of Escherichia coli. J Bacteriol. 2003;185:5697-705 pubmed
    ..Here we describe how increasing signal sequence hydrophobicity routes LamB-LacZ Hyb42-1 to the signal recognition particle (SRP) pathway...
  51. van Nues R, Brown J. Saccharomyces SRP RNA secondary structures: a conserved S-domain and extended Alu-domain. RNA. 2004;10:75-89 pubmed
    The contribution made by the RNA component of signal recognition particle (SRP) to its function in protein targeting is poorly understood. We have generated a complete secondary structure for Saccharomyces cerevisiae SRP RNA, scR1...
  52. Lustig Y, Goldshmidt H, Uliel S, Michaeli S. The Trypanosoma brucei signal recognition particle lacks the Alu-domain-binding proteins: purification and functional analysis of its binding proteins by RNAi. J Cell Sci. 2005;118:4551-62 pubmed
    ..The trypanosome signal recognition particle (SRP) is also unusual in being the first signal recognition particle described in nature to be ..
  53. Halic M, Gartmann M, Schlenker O, Mielke T, Pool M, Sinning I, et al. Signal recognition particle receptor exposes the ribosomal translocon binding site. Science. 2006;312:745-7 pubmed
    Signal sequences of secretory and membrane proteins are recognized by the signal recognition particle (SRP) as they emerge from the ribosome...
  54. Tzvetkova Chevolleau T, Hutin C, Noël L, Goforth R, Carde J, Caffarri S, et al. Canonical signal recognition particle components can be bypassed for posttranslational protein targeting in chloroplasts. Plant Cell. 2007;19:1635-48 pubmed
    The chloroplast signal recognition particle (cpSRP) and its receptor (cpFtsY) target proteins both cotranslationally and posttranslationally to the thylakoids...
  55. Shen K, Zhang X, Shan S. Synergistic actions between the SRP RNA and translating ribosome allow efficient delivery of the correct cargos during cotranslational protein targeting. RNA. 2011;17:892-902 pubmed publisher
    During cotranslational protein targeting by the Signal Recognition Particle (SRP), the correct cargo accelerates stable complex assembly between the SRP and SRP receptor (FtsY) by several orders of magnitude, thus enabling rapid and ..
  56. Tozik I, Huang Q, Zwieb C, Eichler J. Reconstitution of the signal recognition particle of the halophilic archaeon Haloferax volcanii. Nucleic Acids Res. 2002;30:4166-75 pubmed
    The signal recognition particle (SRP) is a ribonucleoprotein complex involved in the recognition and targeting of nascent extracytoplasmic proteins in all three domains of life...
  57. Politz J, Lewandowski L, Pederson T. Signal recognition particle RNA localization within the nucleolus differs from the classical sites of ribosome synthesis. J Cell Biol. 2002;159:411-8 pubmed
    ..In the present study we have investigated how the distribution of signal recognition particle (SRP) RNA within the nucleolus relates to the known sites of ribosomal RNA synthesis, processing, and ..
  58. Eitan A, Bibi E. The core Escherichia coli signal recognition particle receptor contains only the N and G domains of FtsY. J Bacteriol. 2004;186:2492-4 pubmed
    ..proposed that the N-terminal A domain (approximately 200 amino acid residues) of the Escherichia coli signal recognition particle (SRP) receptor, FtsY, is required for membrane targeting...
  59. Lakkaraju A, Luyet P, Parone P, Falguières T, Strub K. Inefficient targeting to the endoplasmic reticulum by the signal recognition particle elicits selective defects in post-ER membrane trafficking. Exp Cell Res. 2007;313:834-47 pubmed
    The signal recognition particle (SRP) is required for protein translocation into the endoplasmic reticulum (ER). With RNA interference we reduced its level about ten-fold in mammalian cells to study its cellular functions...
  60. Stefanovic S, Hegde R. Identification of a targeting factor for posttranslational membrane protein insertion into the ER. Cell. 2007;128:1147-59 pubmed
    ..Thus, TRC40/Asna-1 represents an integral component of a posttranslational pathway of membrane protein insertion whose targeting is mediated by TRC. ..
  61. Stengel K, Holdermann I, Cain P, Robinson C, Wild K, Sinning I. Structural basis for specific substrate recognition by the chloroplast signal recognition particle protein cpSRP43. Science. 2008;321:253-6 pubmed publisher
    ..carry amino-terminal signal sequences that, in cotranslational targeting, are recognized by the signal recognition particle protein SRP54 without sequence specificity...
  62. Hegde R, Kang S. The concept of translocational regulation. J Cell Biol. 2008;182:225-32 pubmed publisher
    ..A conceptual framework for translocational regulation is proposed based on our current mechanistic understanding of ER protein translocation and general principles of regulatory control. ..
  63. Bradshaw N, Neher S, Booth D, Walter P. Signal sequences activate the catalytic switch of SRP RNA. Science. 2009;323:127-30 pubmed publisher
    The signal recognition particle (SRP) recognizes polypeptide chains bearing a signal sequence as they emerge from the ribosome, and then binds its membrane-associated receptor (SR), thereby delivering the ribosome-nascent chain complex ..
  64. Mariappan M, Li X, Stefanovic S, Sharma A, Mateja A, Keenan R, et al. A ribosome-associating factor chaperones tail-anchored membrane proteins. Nature. 2010;466:1120-4 pubmed publisher
    ..Thus, the Bat3 complex acts as a TMD-selective chaperone that effectively channels TA proteins to the TRC40 insertion pathway. ..
  65. Zhang W, Du J, Yu K, Wang T, Yong X, Yu X. Association of potent human antiviral cytidine deaminases with 7SL RNA and viral RNP in HIV-1 virions. J Virol. 2010;84:12903-13 pubmed publisher
    7SL RNA promotes the formation of the signal recognition particle that targets secretory and membrane proteins to the endoplasmic reticulum. 7SL RNA is also selectively packaged by many retroviruses, including HIV-1...
  66. Liu L, Ben Shlomo H, Xu Y, Stern M, Goncharov I, Zhang Y, et al. The trypanosomatid signal recognition particle consists of two RNA molecules, a 7SL RNA homologue and a novel tRNA-like molecule. J Biol Chem. 2003;278:18271-80 pubmed
    ..Here we report that the trypanosomatid signal recognition particle (SRP), unlike all other known SRPs in nature, contains, in addition to the 7SL RNA homologue, a short ..
  67. Egea P, Shan S, Napetschnig J, Savage D, Walter P, Stroud R. Substrate twinning activates the signal recognition particle and its receptor. Nature. 2004;427:215-21 pubmed
    ..As nascent proteins emerge from the ribosome, signal sequences are recognized by the signal recognition particle (SRP), which subsequently associates with its receptor (SR)...
  68. Goforth R, Peterson E, Yuan J, Moore M, Kight A, Lohse M, et al. Regulation of the GTPase cycle in post-translational signal recognition particle-based protein targeting involves cpSRP43. J Biol Chem. 2004;279:43077-84 pubmed
    The chloroplast signal recognition particle consists of a conserved 54-kDa GTPase and a novel 43-kDa chromodomain protein (cpSRP43) that together bind light-harvesting chlorophyll a/b-binding protein (LHCP) to form a soluble targeting ..
  69. Zanen G, Antelmann H, Westers H, Hecker M, van Dijl J, Quax W. FlhF, the third signal recognition particle-GTPase of Bacillus subtilis, is dispensable for protein secretion. J Bacteriol. 2004;186:5956-60 pubmed
    Bacillus subtilis contains three proteins of the signal recognition particle-GTPase family known as Ffh, FtsY, and FlhF. Here we show that FlhF is dispensable for protein secretion, unlike Ffh and FtsY...
  70. Schaletzky J, Rapoport T. Ribosome binding to and dissociation from translocation sites of the endoplasmic reticulum membrane. Mol Biol Cell. 2006;17:3860-9 pubmed
    We have addressed how ribosome-nascent chain complexes (RNCs), associated with the signal recognition particle (SRP), can be targeted to Sec61 translocation channels of the endoplasmic reticulum (ER) membrane when all binding sites are ..
  71. Facey S, Neugebauer S, Krauss S, Kuhn A. The mechanosensitive channel protein MscL is targeted by the SRP to the novel YidC membrane insertion pathway of Escherichia coli. J Mol Biol. 2007;365:995-1004 pubmed
    ..coli MscL protein is synthesized as a polypeptide of 136 amino acid residues and uses the bacterial signal recognition particle (SRP) for membrane targeting...
  72. Francetic O, Buddelmeijer N, Lewenza S, Kumamoto C, Pugsley A. Signal recognition particle-dependent inner membrane targeting of the PulG Pseudopilin component of a type II secretion system. J Bacteriol. 2007;189:1783-93 pubmed
    ..Furthermore, depletion of the Ffh component of the signal recognition particle (SRP) completely abolished PrePulG* processing, without affecting the Sec-dependent export of ..
  73. Maity T, Weeks K. A threefold RNA-protein interface in the signal recognition particle gates native complex assembly. J Mol Biol. 2007;369:512-24 pubmed
    ..is unstructured but forms a compact core domain and two extended RNA-binding loops upon binding the signal recognition particle (SRP) RNA...
  74. Lewis N, Marty N, Kathir K, Rajalingam D, Kight A, Daily A, et al. A dynamic cpSRP43-Albino3 interaction mediates translocase regulation of chloroplast signal recognition particle (cpSRP)-targeting components. J Biol Chem. 2010;285:34220-30 pubmed publisher
    The chloroplast signal recognition particle (cpSRP) and its receptor, chloroplast FtsY (cpFtsY), form an essential complex with the translocase Albino3 (Alb3) during post-translational targeting of light-harvesting chlorophyll-binding ..
  75. Lu Y, Qi H, Hyndman J, Ulbrandt N, Teplyakov A, Tomasevic N, et al. Evidence for a novel GTPase priming step in the SRP protein targeting pathway. EMBO J. 2001;20:6724-34 pubmed
    Protein targeting by the signal recognition particle (SRP) pathway requires the interaction of two homologous GTPases that reciprocally regulate each other's GTPase activity, the SRP signal peptide- binding subunit (SRP54) and the SRP ..
  76. Kuglstatter A, Oubridge C, Nagai K. Induced structural changes of 7SL RNA during the assembly of human signal recognition particle. Nat Struct Biol. 2002;9:740-4 pubmed
    The eukaryotic signal recognition particle (SRP) is a cytoplasmic ribonucleoprotein particle that targets secretory and membrane proteins to the endoplasmic reticulum...
  77. Schwartz T, Blobel G. Structural basis for the function of the beta subunit of the eukaryotic signal recognition particle receptor. Cell. 2003;112:793-803 pubmed
    ..this process is initiated by targeting the translating ribosome to the endoplasmic reticulum via the signal recognition particle (SRP) and its membrane-associated heterodimeric receptor (SR)...
  78. Spanggord R, Siu F, Ke A, Doudna J. RNA-mediated interaction between the peptide-binding and GTPase domains of the signal recognition particle. Nat Struct Mol Biol. 2005;12:1116-22 pubmed
    The signal recognition particle (SRP) targets nascent proteins to cellular membranes for insertion or secretion by recognizing polypeptides containing an N-terminal signal sequence as they emerge from the ribosome...
  79. Nicchitta C, Lerner R, Stephens S, Dodd R, Pyhtila B. Pathways for compartmentalizing protein synthesis in eukaryotic cells: the template-partitioning model. Biochem Cell Biol. 2005;83:687-95 pubmed
    ..In this review, key findings on this topic are discussed, and the template-partitioning model, describing a hypothetical mechanism for RNA partitioning in the eukaryotic cell, is proposed. ..
  80. Peterson J, Szabady R, Bernstein H. An unusual signal peptide extension inhibits the binding of bacterial presecretory proteins to the signal recognition particle, trigger factor, and the SecYEG complex. J Biol Chem. 2006;281:9038-48 pubmed
    Considerable evidence indicates that the Escherichia coli signal recognition particle (SRP) selectively targets proteins that contain highly hydrophobic signal peptides to the SecYEG complex cotranslationally...
  81. Schaffitzel C, Oswald M, Berger I, Ishikawa T, Abrahams J, Koerten H, et al. Structure of the E. coli signal recognition particle bound to a translating ribosome. Nature. 2006;444:503-6 pubmed
    The prokaryotic signal recognition particle (SRP) targets membrane proteins into the inner membrane...
  82. Koch H, Moser M, Schimz K, Muller M. The integration of YidC into the cytoplasmic membrane of Escherichia coli requires the signal recognition particle, SecA and SecYEG. J Biol Chem. 2002;277:5715-8 pubmed
    ..The integration of YidC was strictly dependent on the signal recognition particle and SecA...
  83. Miller T, Al Lozi M, Lopate G, Pestronk A. Myopathy with antibodies to the signal recognition particle: clinical and pathological features. J Neurol Neurosurg Psychiatry. 2002;73:420-8 pubmed
    To study myopathies with serum antibodies to the signal recognition particle (SRP), an unusual, myositis specific antibody associated syndrome that has not been well characterised pathologically...
  84. Raine A, Ullers R, Pavlov M, Luirink J, Wikberg J, Ehrenberg M. Targeting and insertion of heterologous membrane proteins in E. coli. Biochimie. 2003;85:659-68 pubmed
    ..These heterologous proteins are targeted to E. coli membranes in a signal recognition particle (SRP) dependent manner and inserted into the membrane co-translationally...
  85. Römisch K, Miller F, Dobberstein B, High S. Human autoantibodies against the 54 kDa protein of the signal recognition particle block function at multiple stages. Arthritis Res Ther. 2006;8:R39 pubmed
    The 54 kDa subunit of the signal recognition particle (SRP54) binds to the signal sequences of nascent secretory and membrane proteins and it contributes to the targeting of these precursors to the membrane of the endoplasmic reticulum (..
  86. Mary C, Scherrer A, Huck L, Lakkaraju A, Thomas Y, Johnson A, et al. Residues in SRP9/14 essential for elongation arrest activity of the signal recognition particle define a positively charged functional domain on one side of the protein. RNA. 2010;16:969-79 pubmed publisher
    The signal recognition particle (SRP) is a ubiquitous cytoplasmic ribonucleoprotein complex required for the cotranslational targeting of proteins to the endoplasmic reticulum (ER)...
  87. Saraogi I, Shan S. Molecular mechanism of co-translational protein targeting by the signal recognition particle. Traffic. 2011;12:535-42 pubmed publisher
    The signal recognition particle (SRP) is a key component of the cellular machinery that couples the ongoing synthesis of proteins to their proper localization, and has often served as a paradigm for understanding the molecular basis of ..
  88. Ullers R, Houben E, Raine A, ten Hagen Jongman C, Ehrenberg M, Brunner J, et al. Interplay of signal recognition particle and trigger factor at L23 near the nascent chain exit site on the Escherichia coli ribosome. J Cell Biol. 2003;161:679-84 pubmed
    ..The targeting factor signal recognition particle (SRP) interacts specifically with the signal anchor (SA) sequence in nascent inner membrane proteins (..
  89. Halic M, Becker T, Pool M, Spahn C, Grassucci R, Frank J, et al. Structure of the signal recognition particle interacting with the elongation-arrested ribosome. Nature. 2004;427:808-14 pubmed publisher
    ..It requires that the translating ribosome be targeted to the membrane by the signal recognition particle (SRP), an evolutionarily conserved ribonucleoprotein particle...
  90. Ren Y, Wagner K, Knee D, Aza Blanc P, Nasoff M, Deveraux Q. Differential regulation of the TRAIL death receptors DR4 and DR5 by the signal recognition particle. Mol Biol Cell. 2004;15:5064-74 pubmed
    ..DR5-mediated apoptosis. These experiments revealed that expression of the signal recognition particle (SRP) complex is essential for apoptosis mediated by DR4, but not DR5...
  91. Shan S, Walter P. Co-translational protein targeting by the signal recognition particle. FEBS Lett. 2005;579:921-6 pubmed
    The signal recognition particle (SRP) mediates the co-translational targeting of nascent proteins to the eukaryotic endoplasmic reticulum membrane, or the bacterial plasma membrane...
  92. Kriegs J, Churakov G, Jurka J, BROSIUS J, Schmitz J. Evolutionary history of 7SL RNA-derived SINEs in Supraprimates. Trends Genet. 2007;23:158-61 pubmed
    ..As well as new elements in Tupaia and primates, we also found that the purported ancestral fossil Alu monomer was restricted to Primates, and provide here the first description of a potential chimeric promoter box region in SINEs. ..