lamin type b


Summary: A subclass of ubiquitously-expressed lamins having an acidic isoelectric point. They are found to remain bound to nuclear membranes during mitosis.

Top Publications

  1. Furukawa K, Inagaki H, Hotta Y. Identification and cloning of an mRNA coding for a germ cell-specific A-type lamin in mice. Exp Cell Res. 1994;212:426-30 pubmed
    ..This specific expression and unique structure suggests a role for lamin C2 in determining the organization of nuclear and chromosomal structures during spermatogenesis. ..
  2. Lammerding J, Fong L, Ji J, Reue K, Stewart C, Young S, et al. Lamins A and C but not lamin B1 regulate nuclear mechanics. J Biol Chem. 2006;281:25768-80 pubmed
    ..Our study indicates that lamins A and C are important contributors to the mechanical stiffness of nuclei, whereas lamin B1 contributes to nuclear integrity but not stiffness. ..
  3. de Stanchina E, Gabellini D, Norio P, Giacca M, Peverali F, Riva S, et al. Selection of homeotic proteins for binding to a human DNA replication origin. J Mol Biol. 2000;299:667-80 pubmed
    ..We also show that HOXC10 expression is cell-type-dependent and positively correlates with cell proliferation. ..
  4. Pollard K, Chan E, Grant B, Sullivan K, Tan E, Glass C. In vitro posttranslational modification of lamin B cloned from a human T-cell line. Mol Cell Biol. 1990;10:2164-75 pubmed
    ..This in vitro modification included incorporation of a product of mevalonolactone and required an intact carboxy terminus. ..
  5. Markiewicz E, Ledran M, Hutchison C. Remodelling of the nuclear lamina and nucleoskeleton is required for skeletal muscle differentiation in vitro. J Cell Sci. 2005;118:409-20 pubmed
    ..In myoblasts transfected with the mutant lamins, this complex was disrupted. These data suggest that remodelling of the nucleoskeleton is necessary for skeletal-muscle differentiation and for correct regulation of Rb pathways. ..
  6. Al Haboubi T, Shumaker D, Köser J, Wehnert M, Fahrenkrog B. Distinct association of the nuclear pore protein Nup153 with A- and B-type lamins. Nucleus. 2011;2:500-9 pubmed publisher
    ..Together our results indicate a far more intricate interplay between Nup153 and nuclear lamins than previously accepted. ..
  7. Padiath Q, Fu Y. Autosomal dominant leukodystrophy caused by lamin B1 duplications a clinical and molecular case study of altered nuclear function and disease. Methods Cell Biol. 2010;98:337-57 pubmed publisher
  8. Meijer I, Simoes Lopes A, Laurent S, Katz T, St Onge J, Verlaan D, et al. A novel duplication confirms the involvement of 5q23.2 in autosomal dominant leukodystrophy. Arch Neurol. 2008;65:1496-501 pubmed publisher
    ..However, additional functional studies of lamin B1 overexpression are necessary to elucidate the involvement of lamin B1 in myelination and in degenerative disorders such as ADLD and multiple sclerosis. ..
  9. Padiath Q, Saigoh K, Schiffmann R, Asahara H, Yamada T, Koeppen A, et al. Lamin B1 duplications cause autosomal dominant leukodystrophy. Nat Genet. 2006;38:1114-23 pubmed
    ..This raises the possibility that lamin B may be a link to the autoimmune attack that occurs in multiple sclerosis. ..

More Information


  1. Reddy K, Zullo J, Bertolino E, Singh H. Transcriptional repression mediated by repositioning of genes to the nuclear lamina. Nature. 2008;452:243-7 pubmed publisher
    ..We propose that these molecular interactions may be used to compartmentalize and to limit the accessibility of immunoglobulin loci to transcription and recombination factors. ..
  2. Abdurashidova G, Radulescu S, Sandoval O, Zahariev S, Danailov M, Demidovich A, et al. Functional interactions of DNA topoisomerases with a human replication origin. EMBO J. 2007;26:998-1009 pubmed
    ..Inhibition of topoisomerase I activity abolishes origin firing. Thus, the two topoisomerases are closely associated with the replicative complexes, and DNA topology plays an essential functional role in origin activation. ..
  3. Goldberg M, Harel A, Gruenbaum Y. The nuclear lamina: molecular organization and interaction with chromatin. Crit Rev Eukaryot Gene Expr. 1999;9:285-93 pubmed
    ..Taken together, these studies attribute a role for both the nuclear lamins and the lamina-associated proteins, LAP2 and LBR, in nuclear organization and nuclear assembly. ..
  4. Maske C, Hollinshead M, Higbee N, Bergo M, Young S, Vaux D. A carboxyl-terminal interaction of lamin B1 is dependent on the CAAX endoprotease Rce1 and carboxymethylation. J Cell Biol. 2003;162:1223-32 pubmed
    ..These data show that the organization of the nuclear envelope and lamina is dependent on a mechanism involving the methylation of lamin B1, and they identify a potential mechanism of laminopathy involving a B-type lamin. ..
  5. Vergnes L, Peterfy M, Bergo M, Young S, Reue K. Lamin B1 is required for mouse development and nuclear integrity. Proc Natl Acad Sci U S A. 2004;101:10428-33 pubmed
    ..These mutant mice and cell lines derived from them will be useful models for studying the role of the nuclear lamina in various cellular processes. ..
  6. Zewe M, Höger T, Fink T, Lichter P, Krohne G, Franke W. Gene structure and chromosomal localization of the murine lamin B2 gene. Eur J Cell Biol. 1991;56:342-50 pubmed
    ..Moreover, this lamin gene contains an additional intron in the region encoding the rod domain. Our data are compatible with the evolutionary hypothesis that IF proteins have evolved from a lamin-like ancestor molecule. ..
  7. Moir R, Yoon M, Khuon S, Goldman R. Nuclear lamins A and B1: different pathways of assembly during nuclear envelope formation in living cells. J Cell Biol. 2000;151:1155-68 pubmed
    ..In later stages of G1, FRAP analyses suggest that both green fluorescent protein lamins A and B1 form higher order polymers throughout interphase nuclei. ..
  8. Schumacher J, Reichenzeller M, Kempf T, Schnolzer M, Herrmann H. Identification of a novel, highly variable amino-terminal amino acid sequence element in the nuclear intermediate filament protein lamin B(2) from higher vertebrates. FEBS Lett. 2006;580:6211-6 pubmed
  9. Yang S, Chang S, Yin L, Tu Y, Hu Y, Yoshinaga Y, et al. An absence of both lamin B1 and lamin B2 in keratinocytes has no effect on cell proliferation or the development of skin and hair. Hum Mol Genet. 2011;20:3537-44 pubmed publisher
    ..Lmnb1(?/?)Lmnb2(?/?) keratinocytes did not exhibit aneuploidy, and their growth rate was normal in culture. These studies challenge the concept that B-type lamins are essential for proliferation and vitality of eukaryotic cells. ..
  10. Lin F, Worman H. Structural organization of the human gene (LMNB1) encoding nuclear lamin B1. Genomics. 1995;27:230-6 pubmed
  11. Yang S, Jung H, Coffinier C, Fong L, Young S. Are B-type lamins essential in all mammalian cells?. Nucleus. 2011;2:562-9 pubmed publisher
    ..The absence of either lamin B1 or lamin B2, or the absence of both B-type lamins, results in severe neurodevelopmental abnormalities. ..
  12. Guelen L, Pagie L, Brasset E, Meuleman W, Faza M, Talhout W, et al. Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions. Nature. 2008;453:948-51 pubmed publisher
    ..Taken together, these results demonstrate that the human genome is divided into large, discrete domains that are units of chromosome organization within the nucleus...
  13. Brussino A, Vaula G, Cagnoli C, Mauro A, Pradotto L, Daniele D, et al. A novel family with Lamin B1 duplication associated with adult-onset leucoencephalopathy. J Neurol Neurosurg Psychiatry. 2009;80:237-40 pubmed publisher
    ..The variable phenotypes in the remaining cases lacking LMNB1 defects (five with autosomal dominant transmission) suggest that adult-onset leucoencephalopathies are genetically heterogeneous. ..
  14. Worman H, Fong L, Muchir A, Young S. Laminopathies and the long strange trip from basic cell biology to therapy. J Clin Invest. 2009;119:1825-36 pubmed publisher
    ..Here, we review the laminopathies and the long strange trip from basic cell biology to therapeutic approaches for these diseases. ..
  15. Barascu A, Le Chalony C, Pennarun G, Genet D, Imam N, Lopez B, et al. Oxidative stress induces an ATM-independent senescence pathway through p38 MAPK-mediated lamin B1 accumulation. EMBO J. 2012;31:1080-94 pubmed publisher
    ..These data reveal lamin B1 as a general molecular mediator that controls OS-induced senescence, independent of established Ataxia Telangiectasia Mutated (ATM) roles in OIS. ..
  16. Fuentes Mera L, Rodríguez Muñoz R, González Ramírez R, Garcia Sierra F, Gonzalez E, Mornet D, et al. Characterization of a novel Dp71 dystrophin-associated protein complex (DAPC) present in the nucleus of HeLa cells: members of the nuclear DAPC associate with the nuclear matrix. Exp Cell Res. 2006;312:3023-35 pubmed
    ..The association of members of the nuclear DAPC with the nuclear matrix indicates that they may work as scaffolding proteins involved in nuclear architecture. ..
  17. Zhang C, Clarke P. Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts. Science. 2000;288:1429-32 pubmed
    ..Thus, concentration of Ran-GDP followed by generation of Ran-GTP is sufficient to induce NE assembly. ..
  18. Neri L, Raymond Y, Giordano A, Capitani S, Martelli A. Lamin A is part of the internal nucleoskeleton of human erythroleukemia cells. J Cell Physiol. 1999;178:284-95 pubmed
    ..Thus, our results support the hypothesis that lamin A, but not lamin B1, may be a component of an internal nucleoskeleton in human erythroleukemia cells. ..
  19. Sadaie M, Salama R, Carroll T, Tomimatsu K, Chandra T, Young A, et al. Redistribution of the Lamin B1 genomic binding profile affects rearrangement of heterochromatic domains and SAHF formation during senescence. Genes Dev. 2013;27:1800-8 pubmed publisher
    ..These results suggest that LMNB1 may contribute to senescence in at least two ways due to its uneven genome-wide redistribution: first, through the spatial reorganization of chromatin and, second, through gene repression. ..
  20. de Castro S, Malhas A, Leung K, Gustavsson P, Vaux D, Copp A, et al. Lamin b1 polymorphism influences morphology of the nuclear envelope, cell cycle progression, and risk of neural tube defects in mice. PLoS Genet. 2012;8:e1003059 pubmed publisher
  21. Worman H, Lazaridis I, Georgatos S. Nuclear lamina heterogeneity in mammalian cells. Differential expression of the major lamins and variations in lamin B phosphorylation. J Biol Chem. 1988;263:12135-41 pubmed
  22. Maraldi N, Capanni C, Cenni V, Fini M, Lattanzi G. Laminopathies and lamin-associated signaling pathways. J Cell Biochem. 2011;112:979-92 pubmed publisher
    ..In this review, both aspects of lamin-linked signaling are presented and the major pathways so far implicated in laminopathies are summarized. ..
  23. Brussino A, Vaula G, Cagnoli C, Panza E, Seri M, Di Gregorio E, et al. A family with autosomal dominant leukodystrophy linked to 5q23.2-q23.3 without lamin B1 mutations. Eur J Neurol. 2010;17:541-9 pubmed publisher
    ..This observation suggests that a mutation in an LMNB1 regulatory sequence underlies the variant ADLD phenotype. Thus, adult forms of ADLD linked to 5q23 appear to be more heterogeneous clinically and genetically than previously thought. ..
  24. Furukawa K, Kondo T. Identification of the lamina-associated-polypeptide-2-binding domain of B-type lamin. Eur J Biochem. 1998;251:729-33 pubmed
    ..We found that amino acids in the region of residues 78-258 of the lamin B1 rod domain directly bound with LAP2. The data suggest that LAP2 may modulate the assembly of nuclear lamins. ..
  25. Duband Goulet I, Courvalin J, Buendia B. LBR, a chromatin and lamin binding protein from the inner nuclear membrane, is proteolyzed at late stages of apoptosis. J Cell Sci. 1998;111 ( Pt 10):1441-51 pubmed
    ..As the association of condensed chromatin with the inner nuclear membrane persists until the late stages of apoptosis, we suggest that the chromatin binding protein LBR plays a major role in maintaining this association. ..
  26. Panorchan P, Schafer B, Wirtz D, Tseng Y. Nuclear envelope breakdown requires overcoming the mechanical integrity of the nuclear lamina. J Biol Chem. 2004;279:43462-7 pubmed
    ..These results demonstrate that both mechanical tearing of the lamina and biochemical modification of lamin B1 filaments are required for NE breakdown...
  27. Goss V, Hocevar B, Thompson L, Stratton C, Burns D, Fields A. Identification of nuclear beta II protein kinase C as a mitotic lamin kinase. J Biol Chem. 1994;269:19074-80 pubmed
  28. Coffinier C, Jung H, Nobumori C, Chang S, Tu Y, Barnes R, et al. Deficiencies in lamin B1 and lamin B2 cause neurodevelopmental defects and distinct nuclear shape abnormalities in neurons. Mol Biol Cell. 2011;22:4683-93 pubmed publisher
  29. Ye Q, Worman H. Primary structure analysis and lamin B and DNA binding of human LBR, an integral protein of the nuclear envelope inner membrane. J Biol Chem. 1994;269:11306-11 pubmed
    ..These results demonstrate that LBR is conserved among vertebrate species and that its nucleoplasmic domain can potentially mediate the interaction of both the nuclear lamina and the chromatin with the inner nuclear membrane. ..
  30. Lin F, Worman H. Structural organization of the human gene encoding nuclear lamin A and nuclear lamin C. J Biol Chem. 1993;268:16321-6 pubmed
    ..Analysis of the intron positions in these genes supports the hypothesis that the nuclear lamins and other intermediate filament proteins arose from a common ancestor. ..
  31. Utani K, Kawamoto J, Shimizu N. Micronuclei bearing acentric extrachromosomal chromatin are transcriptionally competent and may perturb the cancer cell phenotype. Mol Cancer Res. 2007;5:695-704 pubmed
    ..Taken together, these results indicate that the expression of genes on DMs can be temporally altered by their incorporation into micronuclei. This may be relevant for a broad spectrum of other extrachromosomal elements. ..
  32. Ji J, Lee R, Vergnes L, Fong L, Stewart C, Reue K, et al. Cell nuclei spin in the absence of lamin b1. J Biol Chem. 2007;282:20015-26 pubmed
    ..These findings demonstrate that lamin B1 serves a fundamental role within the nuclear envelope: anchoring the nucleus to the cytoskeleton...
  33. Dechat T, Shimi T, Adam S, Rusinol A, Andres D, Spielmann H, et al. Alterations in mitosis and cell cycle progression caused by a mutant lamin A known to accelerate human aging. Proc Natl Acad Sci U S A. 2007;104:4955-60 pubmed
    ..These results provide insights into the mechanisms responsible for premature aging and also shed light on the role of lamins in the normal process of human aging. ..
  34. Abdurashidova G, Danailov M, Ochem A, Triolo G, Djeliova V, Radulescu S, et al. Localization of proteins bound to a replication origin of human DNA along the cell cycle. EMBO J. 2003;22:4294-303 pubmed
  35. Maeno H, Sugimoto K, Nakajima N. Genomic structure of the mouse gene (Lmnb1) encoding nuclear lamin B1. Genomics. 1995;30:342-6 pubmed
    ..These data reveal the gene structure of the only remaining unanalyzed mouse somatic lamin gene. Gene structures of all the mouse somatic lamins are compared. ..
  36. Kowluru A. Evidence for the carboxyl methylation of nuclear lamin-B in the pancreatic beta cell. Biochem Biophys Res Commun. 2000;268:249-54 pubmed
  37. Foisner R, Gerace L. Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation. Cell. 1993;73:1267-79 pubmed
    ..Together, these data suggest that LAP 2 may have a key role in initial events of nuclear envelope reassembly, and that both LAP 2 and LAP 1 may be involved in attaching lamins to the nuclear envelope. ..
  38. Falaschi A, Abdurashidova G, Sandoval O, Radulescu S, Biamonti G, Riva S. Molecular and structural transactions at human DNA replication origins. Cell Cycle. 2007;6:1705-12 pubmed
  39. Beaudouin J, Gerlich D, Daigle N, Eils R, Ellenberg J. Nuclear envelope breakdown proceeds by microtubule-induced tearing of the lamina. Cell. 2002;108:83-96 pubmed
    ..NE fragments remaining on chromosomes were removed toward the centrosomes in a microtubule-dependent manner, suggesting a mechanism mediated by a minus-end-directed motor. ..
  40. Zastrow M, Flaherty D, Benian G, Wilson K. Nuclear titin interacts with A- and B-type lamins in vitro and in vivo. J Cell Sci. 2006;119:239-49 pubmed
    ..We conclude that the C-terminus of nuclear titin binds lamins in vivo and might contribute to nuclear organization during interphase. ..
  41. Izumi M, Vaughan O, Hutchison C, Gilbert D. Head and/or CaaX domain deletions of lamin proteins disrupt preformed lamin A and C but not lamin B structure in mammalian cells. Mol Biol Cell. 2000;11:4323-37 pubmed
  42. Jung H, Nobumori C, Goulbourne C, Tu Y, Lee J, Tatar A, et al. Farnesylation of lamin B1 is important for retention of nuclear chromatin during neuronal migration. Proc Natl Acad Sci U S A. 2013;110:E1923-32 pubmed publisher
    ..Thus, farnesylation of lamin B1--but not lamin B2--is crucial for brain development and for retaining chromatin within the bounds of the nuclear lamina during neuronal migration...
  43. Prüfert K, Vogel A, Krohne G. The lamin CxxM motif promotes nuclear membrane growth. J Cell Sci. 2004;117:6105-16 pubmed
    ..Our results indicate that the CxxM motif of B-type lamins has a dual function: it mediates lamin targeting to the inner nuclear membrane thereby promoting nuclear membrane growth. ..
  44. Meuleman W, Peric Hupkes D, Kind J, Beaudry J, Pagie L, Kellis M, et al. Constitutive nuclear lamina-genome interactions are highly conserved and associated with A/T-rich sequence. Genome Res. 2013;23:270-80 pubmed publisher
    ..Taken together, these results reveal that the spatial organization of mammalian genomes is highly conserved and tightly linked to local nucleotide composition. ..
  45. Worman H, Yuan J, Blobel G, Georgatos S. A lamin B receptor in the nuclear envelope. Proc Natl Acad Sci U S A. 1988;85:8531-4 pubmed
    ..This protein may serve as a membrane attachment site for the nuclear lamina by acting as a specific receptor for lamin B. ..
  46. Stierle V, Couprie J, Ostlund C, Krimm I, Zinn Justin S, Hossenlopp P, et al. The carboxyl-terminal region common to lamins A and C contains a DNA binding domain. Biochemistry. 2003;42:4819-28 pubmed
    ..We conclude that the carboxyl-terminal end of lamins A and C binds DNA and suggest that alterations in lamin-DNA interactions may play a role in the pathophysiology of some lamin-linked diseases. ..
  47. Broers J, Machiels B, Kuijpers H, Smedts F, van den Kieboom R, Raymond Y, et al. A- and B-type lamins are differentially expressed in normal human tissues. Histochem Cell Biol. 1997;107:505-17 pubmed
    ..Furthermore, most cells of neuroendocrine origin as well as most hematopoietic cells were negative for A-type lamin antibodies. ..
  48. Tsai M, Wang S, Heidinger J, Shumaker D, Adam S, Goldman R, et al. A mitotic lamin B matrix induced by RanGTP required for spindle assembly. Science. 2006;311:1887-93 pubmed
    ..We propose that lamin B is a structural component of the long-sought-after spindle matrix that promotes microtubule assembly and organization in mitosis. ..
  49. Young S, Jung H, Coffinier C, Fong L. Understanding the roles of nuclear A- and B-type lamins in brain development. J Biol Chem. 2012;287:16103-10 pubmed publisher
    ..The relevance of lamins A and C in the brain remains unclear, but it is intriguing that prelamin A expression in the brain is low and is regulated by miR-9, a brain-specific microRNA. ..
  50. Thangavel S, Mendoza Maldonado R, Tissino E, Sidorova J, Yin J, Wang W, et al. Human RECQ1 and RECQ4 helicases play distinct roles in DNA replication initiation. Mol Cell Biol. 2010;30:1382-96 pubmed publisher
    ..These results indicate that RECQ1 and RECQ4 are integral components of the human replication complex and play distinct roles in DNA replication initiation and replication fork progression in vivo. ..
  51. Comelli L, Marchetti L, Arosio D, Riva S, Abdurashidova G, Beltram F, et al. The homeotic protein HOXC13 is a member of human DNA replication complexes. Cell Cycle. 2009;8:454-9 pubmed
  52. Shah P, Donahue G, Otte G, Capell B, Nelson D, Cao K, et al. Lamin B1 depletion in senescent cells triggers large-scale changes in gene expression and the chromatin landscape. Genes Dev. 2013;27:1787-99 pubmed publisher
    ..Our data illustrate profound chromatin reorganization during senescence and suggest that lamin B1 down-regulation in senescence is a key trigger of global and local chromatin changes that impact gene expression, aging, and cancer. ..
  53. Adam S, Sengupta K, Goldman R. Regulation of nuclear lamin polymerization by importin alpha. J Biol Chem. 2008;283:8462-8 pubmed publisher
    ..These results suggest a role for importin alpha in regulating lamin assembly and possibly modulating the interactions of lamins with lamin-binding proteins. ..