small heat shock proteins


Summary: A family of low molecular weight heat-shock proteins that can serve as MOLECULAR CHAPERONES.

Top Publications

  1. Giorno F, Wolters Arts M, Grillo S, Scharf K, Vriezen W, Mariani C. Developmental and heat stress-regulated expression of HsfA2 and small heat shock proteins in tomato anthers. J Exp Bot. 2010;61:453-62 pubmed publisher
    ..These data suggest that HsfA2 may be directly involved in the activation of protection mechanisms in the tomato anther during heat stress and, thereby, may contribute to tomato fruit set under adverse temperatures...
  2. Tiroli Cepeda A, Ramos C. Heat causes oligomeric disassembly and increases the chaperone activity of small heat shock proteins from sugarcane. Plant Physiol Biochem. 2010;48:108-16 pubmed publisher
    b>Small heat shock proteins (sHsp) constitute an important chaperone family linked to conformational diseases. In plants, sHsps prevent protein aggregation by acting as thermosensors and to enhance cell stress tolerance. SsHsp17...
  3. Basha E, Jones C, Wysocki V, Vierling E. Mechanistic differences between two conserved classes of small heat shock proteins found in the plant cytosol. J Biol Chem. 2010;285:11489-97 pubmed publisher
    The small heat shock proteins (sHSPs) and alpha-crystallins are highly effective, ATP-independent chaperones that can bind denaturing client proteins to prevent their irreversible aggregation...
  4. Jaya N, Garcia V, Vierling E. Substrate binding site flexibility of the small heat shock protein molecular chaperones. Proc Natl Acad Sci U S A. 2009;106:15604-9 pubmed publisher
    b>Small heat shock proteins (sHSPs) serve as a first line of defense against stress-induced cell damage by binding and maintaining denaturing proteins in a folding-competent state...
  5. Laskowska E, Matuszewska E, Kuczynska Wisnik D. Small heat shock proteins and protein-misfolding diseases. Curr Pharm Biotechnol. 2010;11:146-57 pubmed
    b>Small heat shock proteins (sHsps) are molecular chaperones ubiquitously distributed in numerous species, from bacteria to humans...
  6. de Miguel N, Braun N, Bepperling A, Kriehuber T, Kastenmüller A, Buchner J, et al. Structural and functional diversity in the family of small heat shock proteins from the parasite Toxoplasma gondii. Biochim Biophys Acta. 2009;1793:1738-48 pubmed publisher
    b>Small heat shock proteins (sHsps) are ubiquitous molecular chaperones which prevent the nonspecific aggregation of non-native proteins. Five potential sHsps exist in the parasite Toxoplasma gondii...
  7. Chen J, Feige M, Franzmann T, Bepperling A, Buchner J. Regions outside the alpha-crystallin domain of the small heat shock protein Hsp26 are required for its dimerization. J Mol Biol. 2010;398:122-31 pubmed publisher
    b>Small heat shock proteins (sHsps) are a ubiquitous family of molecular chaperones. They form homo-oligomers, composed of mostly 24 subunits...
  8. Ukaji N, Kuwabara C, Kanno Y, Seo M, Takezawa D, Arakawa K, et al. Endoplasmic reticulum-localized small heat shock protein that accumulates in mulberry tree (Morus bombycis Koidz.) during seasonal cold acclimation is responsive to abscisic acid. Tree Physiol. 2010;30:502-13 pubmed publisher
    ..The results suggest that ABA may be involved in the expression of the WAP20 gene in bark tissue of the mulberry tree during seasonal cold acclimation. ..
  9. Bissonnette S, Rivera Rivera I, Sauer R, Baker T. The IbpA and IbpB small heat-shock proteins are substrates of the AAA+ Lon protease. Mol Microbiol. 2010;75:1539-49 pubmed publisher
    ..These results suggest a novel mode for Lon substrate recognition and provide a highly suggestive link between the degradation and sHSP branches of the protein quality-control network. ..

More Information


  1. Bedulina D, Timofeyev M, Zimmer M, Zwirnmann E, Menzel R, Steinberg C. Different natural organic matter isolates cause similar stress response patterns in the freshwater amphipod, Gammarus pulex. Environ Sci Pollut Res Int. 2010;17:261-9 pubmed publisher
  2. Kocabiyik S. Essential structural and functional features of small heat shock proteins in molecular chaperoning process. Protein Pept Lett. 2009;16:613-22 pubmed
    b>Small heat shock proteins are ubiquitously found in all three domains of life, although they are the most poorly conserved family of molecular chaperones...
  3. Robertson A, Headey S, Saunders H, Ecroyd H, Scanlon M, Carver J, et al. Small heat-shock proteins interact with a flanking domain to suppress polyglutamine aggregation. Proc Natl Acad Sci U S A. 2010;107:10424-9 pubmed publisher
  4. Gorog D, Jabr R, Tanno M, Sarafraz N, Clark J, Fisher S, et al. MAPKAPK-2 modulates p38-MAPK localization and small heat shock protein phosphorylation but does not mediate the injury associated with p38-MAPK activation during myocardial ischemia. Cell Stress Chaperones. 2009;14:477-89 pubmed publisher
    MAPKAPK-2 (MK2) is a protein kinase activated downstream of p38-MAPK which phosphorylates the small heat shock proteins HSP27 and alphaB crystallin and modulates p38-MAPK cellular distribution...
  5. Kanai T, Takedomi S, Fujiwara S, Atomi H, Imanaka T. Identification of the Phr-dependent heat shock regulon in the hyperthermophilic archaeon, Thermococcus kodakaraensis. J Biochem. 2010;147:361-70 pubmed publisher
  6. Lucon J, Abedin M, Uchida M, Liepold L, Jolley C, Young M, et al. A click chemistry based coordination polymer inside small heat shock protein. Chem Commun (Camb). 2010;46:264-6 pubmed publisher
  7. Singh B, Rao K, Rao C. Ubiquitin-proteasome-mediated degradation and synthesis of MyoD is modulated by alphaB-crystallin, a small heat shock protein, during muscle differentiation. Biochim Biophys Acta. 2010;1803:288-99 pubmed publisher
    ..Our studies show, inter alia, that alphaB-crystallin modulates myogenesis by altering MyoD level and provide an interesting insight in its role in myogenesis. ..
  8. Lu L, Du Z, Qin M, Wang P, Lan H, Niu X, et al. Pc4, a putative movement protein of Rice stripe virus, interacts with a type I DnaJ protein and a small Hsp of rice. Virus Genes. 2009;38:320-7 pubmed publisher
    ..Interactions between Pc4 and the two proteins were confirmed using coimmunoprecipitation. Implications of the findings that pc4 interacted with two chaperone proteins were discussed...
  9. Tsai Y, Chiang Y, Narberhaus F, Baron C, Lai E. The small heat-shock protein HspL is a VirB8 chaperone promoting type IV secretion-mediated DNA transfer. J Biol Chem. 2010;285:19757-66 pubmed publisher
    ..This study directly links in vitro and in vivo functions of an alpha-Hsp and reveals a novel alpha-Hsp function in T4SS stability and bacterial virulence. ..
  10. Weidmann S, Rieu A, Rega M, Coucheney F, Guzzo J. Distinct amino acids of the Oenococcus oeni small heat shock protein Lo18 are essential for damaged protein protection and membrane stabilization. FEMS Microbiol Lett. 2010;309:8-15 pubmed publisher
    ..Our data strongly suggest that different amino acids are involved in the thermostabilization of proteins and in membrane fluidity regulation and are localized in the alpha-crystallin domain. ..
  11. Tomoyasu T, Tabata A, Nagamune H. Investigation of the chaperone function of the small heat shock protein-AgsA. BMC Biochem. 2010;11:27 pubmed publisher
    ..In addition, the equilibrium between the oligomer and the dimer structures appears to be important for its efficient chaperone activity. ..
  12. Hossain T, Teshiba S, Shigeoka Y, Fujisawa T, Inoko Y, Sakano D, et al. Structural properties of silkworm small heat-shock proteins: sHSP19.9 and sHSP20.8. Biosci Biotechnol Biochem. 2010;74:1556-63 pubmed
    ..sHSP20.8 and sHSP19.9 with dithiothreitol were stable against lyophilization, autoclaving at 120 degrees C, and boiling. ..
  13. Wang Y, Xu X, Wen Z, Li W, Yang B, Whiteley C. Isolation, purification, and properties of a novel small heat shock protein from the hyperthermophile Sulfolobus solfataricus. Appl Biochem Biotechnol. 2010;162:476-85 pubmed publisher
    ..1 protein was able to prevent Candida antarctica lipase B from aggregation for up to 60 min at 80 degrees C. Moreover, the SsHSP14.1 enhanced thermostability of bromelain extending its half-life at 55 degrees C by 67%. ..
  14. Zahur M, Maqbool A, Irfan M, Barozai M, Qaiser U, Rashid B, et al. Functional analysis of cotton small heat shock protein promoter region in response to abiotic stresses in tobacco using Agrobacterium-mediated transient assay. Mol Biol Rep. 2009;36:1915-21 pubmed publisher
    ..These findings further our understanding of the regulation of GHSP26 expression and provide a new drought-inducible promoter system in transgenic plants. ..
  15. Jiang C, Xu J, Zhang H, Zhang X, Shi J, Li M, et al. A cytosolic class I small heat shock protein, RcHSP17.8, of Rosa chinensis confers resistance to a variety of stresses to Escherichia coli, yeast and Arabidopsis thaliana. Plant Cell Environ. 2009;32:1046-59 pubmed publisher
    ..These results suggest that R. chinensis cytosolic class I sHSP (RcHSP17.8) has the ability to confer stress resistance not only to E. coli and yeast but also to plants grown under a wide variety of unfavorable environmental conditions...
  16. Vos M, Kanon B, Kampinga H. HSPB7 is a SC35 speckle resident small heat shock protein. Biochim Biophys Acta. 2009;1793:1343-53 pubmed publisher
    ..The functional divergence between HSPB members seems larger than previously expected and also includes non-canonical members lacking classical chaperone-like functions. ..
  17. Tsai Y, Wang M, Gao C, Kl sener S, Baron C, Narberhaus F, et al. Small heat-shock protein HspL is induced by VirB protein(s) and promotes VirB/D4-mediated DNA transfer in Agrobacterium tumefaciens. Microbiology. 2009;155:3270-80 pubmed publisher
    ..In conclusion, increased HspL abundance is triggered in response to certain VirB protein(s) and plays a role in optimal VirB protein accumulation, VirB/D4-mediated DNA transfer and tumorigenesis...
  18. Cao Z, Jia Z, Liu Y, Wang M, Zhao J, Zheng J, et al. Constitutive expression of ZmsHSP in Arabidopsis enhances their cytokinin sensitivity. Mol Biol Rep. 2010;37:1089-97 pubmed publisher
  19. Maqbool A, Abbas W, Rao A, Irfan M, Zahur M, Bakhsh A, et al. Gossypium arboreum GHSP26 enhances drought tolerance in Gossypium hirsutum. Biotechnol Prog. 2010;26:21-5 pubmed publisher
    ..Transgenic cotton plants showed an enhanced drought tolerance, suggesting that GHSP26 may play a role in plant responsiveness to drought. ..
  20. Lambrecht S, Dhaenens M, Almqvist F, Verdonk P, Verbruggen G, Deforce D, et al. Proteome characterization of human articular chondrocytes leads to novel insights in the function of small heat-shock proteins in chondrocyte homeostasis. Osteoarthritis Cartilage. 2010;18:440-6 pubmed publisher
    ..These findings highlight that small HSPs, especially HSP27, play a prominent role in the maintenance of human articular chondrocyte homeostasis. ..
  21. Pérez Morales D, Ostoa Saloma P, Espinoza B. Trypanosoma cruzi SHSP16: Characterization of an alpha-crystallin small heat shock protein. Exp Parasitol. 2009;123:182-9 pubmed publisher
    ..The protein has an alpha-crystallin domain characteristic of the alpha-crystallin small heat shock proteins and a molecular weight of 15.9kDa, so the protein was designated SHSP16...
  22. Liao P, Lin T, Lan W, Chung J, Hwang S. Duplication of the class I cytosolic small heat shock protein gene and potential functional divergence revealed by sequence variations flanking the {alpha}-crystallin domain in the genus Rhododendron (Ericaceae). Ann Bot. 2010;105:57-69 pubmed publisher
  23. Mizrahi T, Heller J, Goldenberg S, Arad Z. Heat shock proteins and resistance to desiccation in congeneric land snails. Cell Stress Chaperones. 2010;15:351-63 pubmed publisher
  24. Zeng L, Hu Z, Lu W, Tang X, Zhang J, Li T, et al. Small heat shock proteins: recent advances in neuropathy. Curr Neurovasc Res. 2010;7:155-66 pubmed
    b>Small heat shock proteins(sHSPs), with a small molecular mass of 12-43 kDa, are molecular chaperones that protect cells against stress by assisting them in the correct folding of denatured proteins and thus prevent aggregation of ..
  25. Colinet H, Lee S, Hoffmann A. Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster. FEBS J. 2010;277:174-85 pubmed publisher
    ..Parallels are drawn between the stress response networks resulting from heat and cold stress. ..
  26. Shashikumar S, Rajini P. Cypermethrin elicited responses in heat shock protein and feeding in Caenorhabditis elegans. Ecotoxicol Environ Saf. 2010;73:1057-62 pubmed publisher was to explore the potential of cypermethrin, a synthetic pyrethroid insecticide, on induction of small heat shock proteins (Hsp16) and feeding response in transgenic Caenorhabditis elegans PC72 (Hsp16-LacZ)...
  27. Yu Y, Dosanjh L, Lao L, Tan M, Shim B, Luo Y. Cinnamomum cassia bark in two herbal formulas increases life span in Caenorhabditis elegans via insulin signaling and stress response pathways. PLoS ONE. 2010;5:e9339 pubmed publisher
    ..This issue may be addressed through the use of contemporary methodology and validated animal models...
  28. Luján R, Lledias F, Martínez L, Barreto R, Cassab G, Nieto Sotelo J. Small heat-shock proteins and leaf cooling capacity account for the unusual heat tolerance of the central spike leaves in Agave tequilana var. Weber. Plant Cell Environ. 2009;32:1791-803 pubmed publisher
    ..tequilana from high temperatures. ..
  29. Flynn C, Cheung Flynn J, Smoke C, Lowry D, Roberson R, Sheller M, et al. Internalization and intracellular trafficking of a PTD-conjugated anti-fibrotic peptide, AZX100, in human dermal keloid fibroblasts. J Pharm Sci. 2010;99:3100-21 pubmed publisher
    ..Our results reveal novel and interesting properties of PTD4 and offer new insight into the cellular mechanisms facilitating an advanced drug delivery tool. ..
  30. Sheng Q, Xia J, Nie Z, Zhang Y. Cloning, expression, and cell localization of a novel small heat shock protein gene: BmHSP25.4. Appl Biochem Biotechnol. 2010;162:1297-305 pubmed publisher
    ..However, after heat treatment, most of BmHSP25.4 distributed in the cell membrane. After 3 h of recovery following the heat shock treatment, the localization of BmHSP25.4 was the same as that under normal conditions...
  31. Gong W, Yue M, Xie B, Wan F, Guo J. Inhibition of citrate synthase thermal aggregation in vitro by recombinant small heat shock proteins. J Microbiol Biotechnol. 2009;19:1628-34 pubmed
    b>Small heat shock proteins (sHSPs) function as molecular chaperones that protect cells against environmental stresses. In the present study, the genes of hsp17.6 and hsp17...
  32. Li Z, Li X, Yu Q, Xiang Z, Kishino H, Zhang Z. The small heat shock protein (sHSP) genes in the silkworm, Bombyx mori, and comparative analysis with other insect sHSP genes. BMC Evol Biol. 2009;9:215 pubmed publisher
    b>Small heat shock proteins (sHSPs) are products of heat shock response and of other stress responses, and ubiquitous in all three domains of life, archaea, bacteria, and eukarya...
  33. Mymrikov E, Bukach O, Seit Nebi A, Gusev N. The pivotal role of the beta 7 strand in the intersubunit contacts of different human small heat shock proteins. Cell Stress Chaperones. 2010;15:365-77 pubmed publisher
    Human alpha B-crystallin and small heat shock proteins HspB6 and HspB8 were mutated so that all endogenous Cys residues were replaced by Ser and the single Cys residue was inserted in a position homologous to that of Cys137 of human ..
  34. Furnish E, Brophy C, Harris V, Macomson S, Winger J, Head G, et al. Treatment with transducible phosphopeptide analogues of the small heat shock-related protein, HSP20, after experimental subarachnoid hemorrhage: prevention and reversal of delayed decreases in cerebral perfusion. J Neurosurg. 2010;112:631-9 pubmed publisher
    ..Transduction of biologically active motifs of downstream regulators like HSP20 represents a potential novel treatment for SAH. ..
  35. Wen Z, Wang Y, Xu X, Yang B, Li W, Xie M. Importance of a potential salt bridge and hydrophobic core in the function and oligomerization of a small heat shock protein. Protein Pept Lett. 2010;17:751-8 pubmed
    ..solfataricus), is reported herein to function to protect EcoR I from heat-induced inactivation. A predicted salt bridge and hydrophobic interactions were found to be important for this function. ..
  36. Stengel F, Baldwin A, Painter A, Jaya N, Basha E, Kay L, et al. Quaternary dynamics and plasticity underlie small heat shock protein chaperone function. Proc Natl Acad Sci U S A. 2010;107:2007-12 pubmed publisher
    b>Small Heat Shock Proteins (sHSPs) are a diverse family of molecular chaperones that prevent protein aggregation by binding clients destabilized during cellular stress...
  37. Hayashi T, Abe T, Takeda K, Akiyama N, Yohda M, Miki K. Crystallization and heavy-atom derivatization of StHsp14.0, a small heat-shock protein from Sulfolobus tokodaii. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009;65:1007-10 pubmed publisher
    ..1 A(3) Da(-1), assuming the presence of two molecules in the asymmetric unit. Heavy-atom derivative crystals were prepared successfully by the cocrystallization method and are isomorphic to native crystals. ..
  38. Kappé G, Boelens W, de Jong W. Why proteins without an alpha-crystallin domain should not be included in the human small heat shock protein family HSPB. Cell Stress Chaperones. 2010;15:457-61 pubmed publisher
    ..of an alpha-crystallin domain documents the evolutionary relatedness of the ubiquitous family of small heat shock proteins. Sequence and three-dimensional structure provide no evidence for the presence of such a domain in ..
  39. Sao K, Murata M, Fujisaki Y, Umezaki K, Mori T, Niidome T, et al. A novel protease activity assay using a protease-responsive chaperone protein. Biochem Biophys Res Commun. 2009;383:293-7 pubmed publisher
    ..The results clearly demonstrate that this assay is an effective, easy-to-use method for determining protease activities without the requirement of labeling procedures and the use of radioisotopes. ..