soluble n ethylmaleimide sensitive factor attachment proteins


Summary: SNARE binding proteins that facilitate the ATP hydrolysis-driven dissociation of the SNARE complex. They are required for the binding of N-ETHYLMALEIMIDE-SENSITIVE PROTEIN (NSF) to the SNARE complex which also stimulates the ATPASE activity of NSF. They are unrelated structurally to SNAP-25 PROTEIN.

Top Publications

  1. Stroupe C, Hickey C, Mima J, Burfeind A, Wickner W. Minimal membrane docking requirements revealed by reconstitution of Rab GTPase-dependent membrane fusion from purified components. Proc Natl Acad Sci U S A. 2009;106:17626-33 pubmed publisher
    ..We use this reconstituted system to show that SNAREs and Sec17p/Sec18p, and Ypt7p and the HOPS complex, are required for stable intermembrane interactions and that the three vacuolar Q-SNAREs are sufficient for these interactions. ..
  2. Zhao C, Matveeva E, Ren Q, Whiteheart S. Dissecting the N-ethylmaleimide-sensitive factor: required elements of the N and D1 domains. J Biol Chem. 2010;285:761-72 pubmed publisher
    ..Taken together, these data establish functional roles for many of the structural elements of the N domain and of the D1 ATP-binding site of NSF. ..
  3. Winter U, Chen X, Fasshauer D. A conserved membrane attachment site in alpha-SNAP facilitates N-ethylmaleimide-sensitive factor (NSF)-driven SNARE complex disassembly. J Biol Chem. 2009;284:31817-26 pubmed publisher
    ..This implies that the disassembly machinery is adapted to attack membrane-bound SNARE complexes, probably in their relaxed cis-configuration. ..
  4. Beites C, Campbell K, Trimble W. The septin Sept5/CDCrel-1 competes with alpha-SNAP for binding to the SNARE complex. Biochem J. 2005;385:347-53 pubmed
    ..This interaction is occluded by the binding of alpha-SNAP, suggesting that Sept5 may regulate the availability of SNARE proteins through its interaction with syntaxin and the 7 S complex. ..
  5. Darios F, Ruipérez V, Lopez I, Villanueva J, Gutierrez L, Davletov B. Alpha-synuclein sequesters arachidonic acid to modulate SNARE-mediated exocytosis. EMBO Rep. 2010;11:528-33 pubmed publisher
    ..Alpha-synuclein sequesters arachidonic acid and thereby blocks the activation of SNAREs. Our data provide mechanistic insights into the action of alpha-synuclein in the modulation of neurotransmission. ..
  6. San Pietro E, Capestrano M, Polishchuk E, DiPentima A, Trucco A, Zizza P, et al. Group IV phospholipase A(2)alpha controls the formation of inter-cisternal continuities involved in intra-Golgi transport. PLoS Biol. 2009;7:e1000194 pubmed publisher
    ..These findings identify cPLA(2)alpha as the first component of the machinery that is responsible for the formation of intercisternal tubular continuities and support a role for these continuities in transport through the Golgi complex...
  7. Batiz L, De Blas G, Michaut M, Ramírez A, Rodríguez F, Ratto M, et al. Sperm from hyh mice carrying a point mutation in alphaSNAP have a defect in acrosome reaction. PLoS ONE. 2009;4:e4963 pubmed publisher
    ..We conclude that the M105I mutation affects the expression and also the function of alphaSNAP, and that a fully functional alphaSNAP is necessary for acrosomal exocytosis, a key event in fertilization. ..
  8. McMahon H, Sudhof T. Synaptic core complex of synaptobrevin, syntaxin, and SNAP25 forms high affinity alpha-SNAP binding site. J Biol Chem. 1995;270:2213-7 pubmed
    ..Thus, alpha-SNAP probably functions in a late step of the membrane fusion reaction after the formation of the synaptobrevin-syntaxin-SNAP25 core complex. ..
  9. Ohno Y, Ishihara S, Terada R, Kikuta M, Sofue N, Kawai Y, et al. Preferential increase in the hippocampal synaptic vesicle protein 2A (SV2A) by pentylenetetrazole kindling. Biochem Biophys Res Commun. 2009;390:415-20 pubmed publisher
    ..These findings suggest that PTZ kindling preferentially elevates SV2A expression in the hippocampus probably as a compensatory mechanism to activate the inhibitory neurotransmission. ..

More Information


  1. Batiz L, Roales Buján R, Rodríguez Pérez L, Matas I, Páez P, Roque M, et al. A simple PCR-based genotyping method for M105I mutation of alpha-SNAP enhances the study of early pathological changes in hyh phenotype. Mol Cell Probes. 2009;23:281-90 pubmed publisher
    ..The genotyping method described here enhances the potentiality of hyh mouse as a unique in vivo model to study the role of membrane trafficking in different developmental and physiological processes. ..
  2. Hashizume K, Cheng Y, Hutton J, Chiu C, Carr C. Yeast Sec1p functions before and after vesicle docking. Mol Biol Cell. 2009;20:4673-85 pubmed publisher
  3. Mima J, Wickner W. Phosphoinositides and SNARE chaperones synergistically assemble and remodel SNARE complexes for membrane fusion. Proc Natl Acad Sci U S A. 2009;106:16191-6 pubmed publisher
    ..This ternary synergy of phosphoinositides and 2 SNARE chaperone systems is required for rapid fusion. ..
  4. Radke A, Reynolds L, Melo R, Dvorak A, Weller P, Spencer L. Mature human eosinophils express functional Notch ligands mediating eosinophil autocrine regulation. Blood. 2009;113:3092-101 pubmed publisher
  5. Parashuraman S, Madan R, Mukhopadhyay A. NSF independent fusion of Salmonella-containing late phagosomes with early endosomes. FEBS Lett. 2010;584:1251-6 pubmed publisher
    ..Furthermore, LSLP fuses with EE in absence of NEM-sensitive fusion factor (NSF) as well as in the presence of NSF:D1EQ mutant demonstrating that LSLP fusion with EE is NSF independent. ..
  6. Xu H, Jun Y, Thompson J, Yates J, Wickner W. HOPS prevents the disassembly of trans-SNARE complexes by Sec17p/Sec18p during membrane fusion. EMBO J. 2010;29:1948-60 pubmed publisher
    ..HOPS thus directs the Sec17p/Sec18p chaperone system to maximize functional trans-SNARE complex for membrane fusion, a new role of tethering factors during membrane traffic. ..
  7. Burgalossi A, Jung S, Meyer G, Jockusch W, Jahn O, Taschenberger H, et al. SNARE protein recycling by ?SNAP and ?SNAP supports synaptic vesicle priming. Neuron. 2010;68:473-87 pubmed publisher
    ..Our analysis of ?- and ?SNAP deletion mutant neurons shows that the two NSF cofactors support synaptic vesicle priming by determining the availability of free SNARE components, particularly during phases of high synaptic activity. ..
  8. Li X, Zhang J, Wang Y, Ji J, Yang F, Wan C, et al. Association study on the NAPG gene and bipolar disorder in the Chinese Han population. Neurosci Lett. 2009;457:159-62 pubmed publisher
    ..Haplotype T-A-T of rs473938-rs2290279-rs495484 was defined by confidence intervals algorithm and had a p value of 0.0038 after 100,000 permutations. Our study supports NAPG as a candidate for susceptibility to bipolar disorder. ..
  9. Schilde C, Schönemann B, Sehring I, Plattner H. Distinct subcellular localization of a group of synaptobrevin-like SNAREs in Paramecium tetraurelia and effects of silencing SNARE-specific chaperone NSF. Eukaryot Cell. 2010;9:288-305 pubmed publisher
    ..The distinct distributions of these SNAREs emphasize the considerable differentiation of membrane trafficking, particularly along the endo-/phagocytic pathway, in this protozoan...
  10. Arasaki K, Roy C. Legionella pneumophila promotes functional interactions between plasma membrane syntaxins and Sec22b. Traffic. 2010;11:587-600 pubmed publisher
    ..pneumophila promotes pairing of plasma membrane syntaxins and Sec22b could provide unique insight into how the secretory vesicles could provide an additional membrane reserve subverted during phagosome maturation. ..
  11. Hickey C, Wickner W. HOPS initiates vacuole docking by tethering membranes before trans-SNARE complex assembly. Mol Biol Cell. 2010;21:2297-305 pubmed publisher
    ..SNAREs further stabilize the associations of HOPS-tethered membranes. HOPS then protects newly formed trans-SNARE complexes from disassembly by Sec17p/Sec18p. ..
  12. Wu Z, Chao C. Knockdown of NAPA using short-hairpin RNA sensitizes cancer cells to cisplatin: implications to overcome chemoresistance. Biochem Pharmacol. 2010;80:827-37 pubmed
    ..Taken together, these observations suggest that NAPA represents a target of cisplatin, and that knockdown of NAPA may improve cisplatin-based cancer therapy. ..
  13. Kaneko Y, Tamura K, Totsukawa G, Kondo H. Isolation of a point-mutated p47 lacking binding affinity to p97ATPase. FEBS Lett. 2010;584:3873-7 pubmed publisher
    ..In addition, mutation corresponding to the p47 F253S mutation in p37 and ufd1 also abolished their binding ability to p97. ..