vesicular transport proteins


Summary: A broad category of proteins involved in the formation, transport and dissolution of TRANSPORT VESICLES. They play a role in the intracellular transport of molecules contained within membrane vesicles. Vesicular transport proteins are distinguished from MEMBRANE TRANSPORT PROTEINS, which move molecules across membranes, by the mode in which the molecules are transported.

Top Publications

  1. Zhao Y, Liu J, Yang C, Capraro B, Baumgart T, Bradley R, et al. Exo70 generates membrane curvature for morphogenesis and cell migration. Dev Cell. 2013;26:266-78 pubmed publisher
    ..Exo70 thus represents a membrane-bending protein that may couple actin dynamics and plasma membrane remodeling for morphogenesis. ..
  2. Mohammadi S, Isberg R. Cdc42 interacts with the exocyst complex to promote phagocytosis. J Cell Biol. 2013;200:81-93 pubmed publisher
    ..Furthermore, disruption of exocyst function through Exo70 depletion led to a defect in large particle internalization, thereby establishing a functional role for the exocyst complex during phagocytosis. ..
  3. Hedskog L, Pinho C, Filadi R, Rönnbäck A, Hertwig L, Wiehager B, et al. Modulation of the endoplasmic reticulum-mitochondria interface in Alzheimer's disease and related models. Proc Natl Acad Sci U S A. 2013;110:7916-21 pubmed publisher
    ..Our data suggest an important role of ER-mitochondria contacts and cross-talk in AD pathology. ..
  4. Bornert O, Møller T, Boeuf J, Candusso M, Wagner R, Martinez K, et al. Identification of a novel protein-protein interaction motif mediating interaction of GPCR-associated sorting proteins with G protein-coupled receptors. PLoS ONE. 2013;8:e56336 pubmed publisher
  5. Kim J, Kim Y, Fang C, Russell R, Kim J, Fan W, et al. Differential regulation of distinct Vps34 complexes by AMPK in nutrient stress and autophagy. Cell. 2013;152:290-303 pubmed publisher
    ..Our study reveals an intricate molecular regulation of Vps34 complexes by AMPK in nutrient stress response and autophagy. ..
  6. Koreishi M, Yu S, Oda M, Honjo Y, Satoh A. CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking. PLoS ONE. 2013;8:e54382 pubmed publisher
    ..These results suggest that Sec31 phosphorylation by CK2 controls the duration of COPII vesicle formation, which regulates ER-to-Golgi trafficking...
  7. Snyder J, Rochelle L, Lyerly H, Caron M, BARAK L. Constitutive internalization of the leucine-rich G protein-coupled receptor-5 (LGR5) to the trans-Golgi network. J Biol Chem. 2013;288:10286-97 pubmed publisher
    ..The constitutive internalization of LGR5 to the TGN suggests the existence of novel biochemical roles for its Wnt pathway related, but ill defined signaling program. ..
  8. Piotrowski J, Gomez T, Schoon R, Mangalam A, Billadeau D. WASH knockout T cells demonstrate defective receptor trafficking, proliferation, and effector function. Mol Cell Biol. 2013;33:958-73 pubmed publisher
    ..Aberrant trafficking of these key T cell proteins may potentially lead to attenuated proliferation and effector function. ..
  9. Seaman M. The retromer complex - endosomal protein recycling and beyond. J Cell Sci. 2012;125:4693-702 pubmed publisher
    ..In this Commentary, I will highlight recent advances in the understanding of retromer-mediated endosomal protein sorting and discuss how retromer contributes to a diverse set of physiological processes...

More Information


  1. Steinberg F, Gallon M, Winfield M, Thomas E, Bell A, Heesom K, et al. A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport. Nat Cell Biol. 2013;15:461-71 pubmed publisher
    ..Our data identify the SNX27-retromer as a major endosomal recycling hub required to maintain cellular nutrient homeostasis. ..
  2. Epp N, Ungermann C. The N-terminal domains of Vps3 and Vps8 are critical for localization and function of the CORVET tethering complex on endosomes. PLoS ONE. 2013;8:e67307 pubmed publisher
  3. Graham S, Wartosch L, Gray S, Scourfield E, Deane J, Luzio J, et al. Structural basis of Vps33A recruitment to the human HOPS complex by Vps16. Proc Natl Acad Sci U S A. 2013;110:13345-50 pubmed publisher
    ..The Vps33A-Vps16 complex provides a structural framework for studying the association between Sec1/Munc18 proteins and tethering complexes. ..
  4. López Sánchez I, Garcia Marcos M, Mittal Y, Aznar N, Farquhar M, Ghosh P. Protein kinase C-theta (PKC?) phosphorylates and inhibits the guanine exchange factor, GIV/Girdin. Proc Natl Acad Sci U S A. 2013;110:5510-5 pubmed publisher
    ..This phosphoevent constitutes the only regulatory pathway described for terminating signaling by any of the growing family of nonreceptor GEFs that modulate G-protein activity. ..
  5. Farre J, Burkenroad A, Burnett S, Subramani S. Phosphorylation of mitophagy and pexophagy receptors coordinates their interaction with Atg8 and Atg11. EMBO Rep. 2013;14:441-9 pubmed publisher
    ..We present a common model for the phosphoregulation of selective autophagy receptors...
  6. Seaman M, Gautreau A, Billadeau D. Retromer-mediated endosomal protein sorting: all WASHed up!. Trends Cell Biol. 2013;23:522-8 pubmed publisher
    ..In this review, recent progress in understanding the role of the WASH complex along with retromer in endosomal protein sorting is discussed. ..
  7. Hayden J, Williams M, Granich A, Ahn H, Tenay B, Lukehart J, et al. Vps1 in the late endosome-to-vacuole traffic. J Biosci. 2013;38:73-83 pubmed
    ..Together, we propose that Vps1 is required for correct and efficient trafficking from the late endosomal compartments to the vacuole. ..
  8. Park L, Thomason P, Zech T, King J, Veltman D, Carnell M, et al. Cyclical action of the WASH complex: FAM21 and capping protein drive WASH recycling, not initial recruitment. Dev Cell. 2013;24:169-81 pubmed publisher
    ..Recycling is driven by FAM21's interaction with capping protein, which couples the WASH complex to dynamic actin on vesicles...
  9. Helfer E, Harbour M, Henriot V, Lakisic G, Sousa Blin C, Volceanov L, et al. Endosomal recruitment of the WASH complex: active sequences and mutations impairing interaction with the retromer. Biol Cell. 2013;105:191-207 pubmed publisher
    ..The interaction between these two endosomal machineries, the WASH complex and the retromer, is likely to play a critical role in forming platforms at the surface of endosomes for efficient sorting of cargoes. ..
  10. Lin C, Ear J, Pavlova Y, Mittal Y, Kufareva I, Ghassemian M, et al. Tyrosine phosphorylation of the G?-interacting protein GIV promotes activation of phosphoinositide 3-kinase during cell migration. Sci Signal. 2011;4:ra64 pubmed publisher
    ..These results suggest a mechanism by which multiple receptors activate PI3K through tyrosine phosphorylation of GIV, thereby making the GIV-PI3K interaction a potential therapeutic target within the PI3K-Akt pathway. ..
  11. Hou W, Liua I, Huang S, Huang J. CRSBP-1/LYVE-1 ligands stimulate contraction of the CRSBP-1-associated ER network in lymphatic endothelial cells. FEBS Lett. 2012;586:1480-7 pubmed publisher
    ..These results suggest that ligand-stimulated ER contraction is associated with ligand-stimulated contraction in LECs. ..
  12. Huet S, Fanget I, Jouannot O, Meireles P, Zeiske T, Larochette N, et al. Myrip couples the capture of secretory granules by the actin-rich cell cortex and their attachment to the plasma membrane. J Neurosci. 2012;32:2564-77 pubmed publisher
    ..We conclude that Myrip couples the retention of SGs in the cell cortex, their transport to the PM, and their attachment to the PM, and thus promotes secretion. These three steps of the secretory process are thus intimately coordinated...
  13. Motley A, Nuttall J, Hettema E. Pex3-anchored Atg36 tags peroxisomes for degradation in Saccharomyces cerevisiae. EMBO J. 2012;31:2852-68 pubmed publisher
    ..We conclude that Pex3 recruits the pexophagy receptor Atg36. This reinforces the pivotal role played by Pex3 in coordinating the size of the peroxisome pool, and establishes its role in pexophagy in S. cerevisiae...
  14. Rönnberg E, Pejler G. Serglycin: the master of the mast cell. Methods Mol Biol. 2012;836:201-17 pubmed publisher
    ..Hence, serglycin has a major function in maintaining mast cell secretory granule homeostasis. ..
  15. Smaczynska de Rooij I, Allwood E, Mishra R, Booth W, Aghamohammadzadeh S, Goldberg M, et al. Yeast dynamin Vps1 and amphiphysin Rvs167 function together during endocytosis. Traffic. 2012;13:317-28 pubmed publisher
    ..In vitro, an Rvs161/Rvs167 heterodimer can disassemble Vps1 oligomers. Overall, the data support the idea that Vps1 and the amphiphysins function together to mediate scission during endocytosis in yeast. ..
  16. Brozzi F, Diraison F, Lajus S, Rajatileka S, Philips T, Regazzi R, et al. Molecular mechanism of myosin Va recruitment to dense core secretory granules. Traffic. 2012;13:54-69 pubmed publisher
    ..This study demonstrates that multiple binding partners of BR-MyoVa regulate SG transport, and this molecular mechanism is universally used by neuronal, endocrine and neuroendocrine cells...
  17. Helbig K, Eyre N, Yip E, Narayana S, Li K, Fiches G, et al. The antiviral protein viperin inhibits hepatitis C virus replication via interaction with nonstructural protein 5A. Hepatology. 2011;54:1506-17 pubmed publisher
    ..This highlights the complexity of the host control of viral replication by interferon-stimulated gene expression. ..
  18. Jin L, Pahuja K, Wickliffe K, Gorur A, Baumgärtel C, Schekman R, et al. Ubiquitin-dependent regulation of COPII coat size and function. Nature. 2012;482:495-500 pubmed publisher
    ..As a result, ubiquitylation by CUL3-KLHL12 is essential for collagen export, yet less important for the transport of small cargo. We conclude that monoubiquitylation controls the size and function of a vesicle coat. ..
  19. Liu J, Guo W. The exocyst complex in exocytosis and cell migration. Protoplasma. 2012;249:587-97 pubmed publisher
    ..Then, we will discuss the specific functions of the exocyst in exocytosis in various cell types. Finally, we will review the emerging roles of the exocyst during cell migration and tumor cell invasion. ..
  20. Dickman D, Tong A, Davis G. Snapin is critical for presynaptic homeostatic plasticity. J Neurosci. 2012;32:8716-24 pubmed publisher
    ..Finally, we provide genetic evidence that the interaction of Snapin with SNAP25, a component of the SNARE complex, is also involved in synaptic homeostasis. ..
  21. Zhou B, Wu Y, Lin X. Retromer regulates apical-basal polarity through recycling Crumbs. Dev Biol. 2011;360:87-95 pubmed publisher
    ..Our data presented here uncover the role of retromer in regulating apical-basal polarity in epithelial cells and identify retromer as a novel regulator of Crb recycling. ..
  22. Bendezú F, Vincenzetti V, Martin S. Fission yeast Sec3 and Exo70 are transported on actin cables and localize the exocyst complex to cell poles. PLoS ONE. 2012;7:e40248 pubmed publisher
    ..These data suggest that the exocyst holocomplex, including Sec3 and Exo70, is present on exocytic vesicles, which can reach cell poles by either myosin-driven transport or random walk. ..
  23. Guo J, Sun Q, Lv Z, Yu R, Li K, Zhang Y, et al. VPS35 gene variants are not associated with Parkinson's disease in the mainland Chinese population. Parkinsonism Relat Disord. 2012;18:983-5 pubmed publisher
    ..None of the 1011 sporadic PD patients and 1016 controls carried the VPS35 gene c.1858G > A (p.Asp620Asn) mutation. Our data indicated that the VPS35 variants are not associated with PD in the mainland Chinese population. ..
  24. Suzuki K, Morimoto M, Kondo C, Ohsumi Y. Selective autophagy regulates insertional mutagenesis by the Ty1 retrotransposon in Saccharomyces cerevisiae. Dev Cell. 2011;21:358-65 pubmed publisher
    ..We propose that selective autophagy safeguards genome integrity against excessive insertional mutagenesis caused during nutrient starvation by transposable elements in eukaryotic cells. ..
  25. Gillon A, Latham C, Miller E. Vesicle-mediated ER export of proteins and lipids. Biochim Biophys Acta. 2012;1821:1040-9 pubmed publisher
    ..This article is part of a Special Issue entitled Lipids and Vesicular Transport...
  26. Alfaro G, Johansen J, Dighe S, Duamel G, Kozminski K, Beh C. The sterol-binding protein Kes1/Osh4p is a regulator of polarized exocytosis. Traffic. 2011;12:1521-36 pubmed publisher
    ..These results support a model in which Osh4p acts as a sterol-dependent regulator of polarized vesicle transport, as opposed to being a sterol-transfer protein. ..
  27. Zucchi P, Zick M. Membrane fusion catalyzed by a Rab, SNAREs, and SNARE chaperones is accompanied by enhanced permeability to small molecules and by lysis. Mol Biol Cell. 2011;22:4635-46 pubmed publisher
  28. Ren J, Guo W. ERK1/2 regulate exocytosis through direct phosphorylation of the exocyst component Exo70. Dev Cell. 2012;22:967-78 pubmed publisher
    ..ERK1/2 phosphorylation of Exo70 may thus coordinate exocytosis with other cellular events in response to growth factor signaling. ..
  29. Liu T, Gomez T, Sackey B, Billadeau D, Burd C. Rab GTPase regulation of retromer-mediated cargo export during endosome maturation. Mol Biol Cell. 2012;23:2505-15 pubmed publisher
    ..Thus retromer has dual roles in retrograde cargo export and in controlling the fusion dynamics of the late endovacuolar system. ..
  30. Cai B, Caplan S, Naslavsky N. cPLA2? and EHD1 interact and regulate the vesiculation of cholesterol-rich, GPI-anchored, protein-containing endosomes. Mol Biol Cell. 2012;23:1874-88 pubmed publisher
    ..We speculate that cPLA2? induces membrane curvature and allows EHD1, possibly in the context of a complex, to sever the curved membranes into vesicles. ..
  31. Tiwari A, Jung J, Inamdar S, Brown C, Goel A, Choudhury A. Endothelial cell migration on fibronectin is regulated by syntaxin 6-mediated alpha5beta1 integrin recycling. J Biol Chem. 2011;286:36749-61 pubmed publisher
    ..These syntaxin 6-regulated membrane trafficking events control outside-in signaling via haptotactic and chemotactic mechanisms. ..
  32. Jakobsson J, Ackermann F, Andersson F, Larhammar D, Low P, Brodin L. Regulation of synaptic vesicle budding and dynamin function by an EHD ATPase. J Neurosci. 2011;31:13972-80 pubmed publisher
    ..Based on our in vitro observations, we suggest that l-EHD acts to limit the formation of long, unproductive dynamin helices, thereby promoting vesicle budding. ..
  33. Morgera F, Sallah M, Dubuke M, Gandhi P, Brewer D, Carr C, et al. Regulation of exocytosis by the exocyst subunit Sec6 and the SM protein Sec1. Mol Biol Cell. 2012;23:337-46 pubmed publisher
    ..Therefore, upon vesicle arrival, Sec6 is proposed to release Sec9 in favor of Sec6-exocyst assembly and to simultaneously recruit Sec1 to sites of secretion for coordinated SNARE complex formation and membrane fusion. ..
  34. Thapa N, Sun Y, Schramp M, Choi S, Ling K, Anderson R. Phosphoinositide signaling regulates the exocyst complex and polarized integrin trafficking in directionally migrating cells. Dev Cell. 2012;22:116-30 pubmed publisher
    ..These results reveal how phosphoinositide generation orchestrates polarized trafficking of integrin in coordination with talin that links integrins to the actin cytoskeleton, processes that are required for directional migration...
  35. Gonzalez Deniselle M, Carreras M, Garay L, Gargiulo Monachelli G, Meyer M, Poderoso J, et al. Progesterone prevents mitochondrial dysfunction in the spinal cord of wobbler mice. J Neurochem. 2012;122:185-95 pubmed publisher
    ..Thus, progesterone neuroprotection decreases mitochondriopathy of Wobbler mouse cervical spinal cord. ..
  36. Jiu Y, Jin C, Liu Y, Holmberg C, Jantti J. Exocyst subunits Exo70 and Exo84 cooperate with small GTPases to regulate behavior and endocytic trafficking in C. elegans. PLoS ONE. 2012;7:e32077 pubmed publisher
    ..The findings identify novel, potential regulators for exocyst function and show that exoc-7 and exoc-8 are functionally linked to rab-10 in endosomal trafficking in intestinal epithelial cells in C. elegans. ..
  37. van Weering J, Sessions R, Traer C, Kloer D, Bhatia V, Stamou D, et al. Molecular basis for SNX-BAR-mediated assembly of distinct endosomal sorting tubules. EMBO J. 2012;31:4466-80 pubmed publisher
    ..Our data provide insight into the molecular mechanism that generates and organizes the tubular endosomal network...
  38. Neto H, Gould G. The regulation of abscission by multi-protein complexes. J Cell Sci. 2011;124:3199-207 pubmed publisher
    ..However, cell division in other systems, such as plants and Archae, is also considered, reflecting the mechanistic conservation of membrane-scission processes during cell division. ..
  39. Yoshibori M, Yorimitsu T, Sato K. Involvement of the penta-EF-hand protein Pef1p in the Ca2+-dependent regulation of COPII subunit assembly in Saccharomyces cerevisiae. PLoS ONE. 2012;7:e40765 pubmed publisher
    ..Our results provide further evidence for a linkage between Ca(2+)-dependent signaling and ER-to-Golgi trafficking, but its mechanism of action in yeast seems to be different from the mechanism reported for its mammalian homolog ALG-2. ..
  40. Zhou B, Cai Q, Xie Y, Sheng Z. Snapin recruits dynein to BDNF-TrkB signaling endosomes for retrograde axonal transport and is essential for dendrite growth of cortical neurons. Cell Rep. 2012;2:42-51 pubmed publisher
    ..Our study indicates that Snapin-dynein coupling is one of the primary mechanisms driving BDNF-TrkB retrograde transport, thus providing mechanistic insights into the regulation of neuronal growth and survival. ..
  41. Harbour M, Breusegem S, Seaman M. Recruitment of the endosomal WASH complex is mediated by the extended 'tail' of Fam21 binding to the retromer protein Vps35. Biochem J. 2012;442:209-20 pubmed publisher
    ..Additionally, overexpression of the Fam21-tail results in cell-spreading defects, implicating the activity of the WASH complex in regulating the mobilization of membrane into the endosome-to-cell surface pathway. ..
  42. Lua S, Qin H, Lim L, Shi J, Gupta G, Song J. Structural, stability, dynamic and binding properties of the ALS-causing T46I mutant of the hVAPB MSP domain as revealed by NMR and MD simulations. PLoS ONE. 2011;6:e27072 pubmed publisher
  43. Hsu V, Bai M, Li J. Getting active: protein sorting in endocytic recycling. Nat Rev Mol Cell Biol. 2012;13:323-8 pubmed publisher
    ..Further articulation of this emerging trend has far-reaching implications for a deeper understanding of many physiological and pathological events that require recycling. ..
  44. Gomez T, Gorman J, de Narvajas A, Koenig A, Billadeau D. Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks. Mol Biol Cell. 2012;23:3215-28 pubmed publisher
  45. Russo R, Gambale A, Esposito M, Serra M, Troiano A, De Maggio I, et al. Two founder mutations in the SEC23B gene account for the relatively high frequency of CDA II in the Italian population. Am J Hematol. 2011;86:727-32 pubmed publisher
    ..Instead, R14W may have originated in Southern Italy. The relatively high frequency of the R14W mutation may account for the known increased prevalence of CDA II in Italy. ..
  46. Zong M, Wu X, Chan C, Choi M, Chan H, Tanner J, et al. The adaptor function of TRAPPC2 in mammalian TRAPPs explains TRAPPC2-associated SEDT and TRAPPC9-associated congenital intellectual disability. PLoS ONE. 2011;6:e23350 pubmed publisher
    ..TRAPPC2 serves as an adaptor for the formation of TRAPPII or TRAPPIII in mammalian cells. The mammalian equivalent of TRAPPII is likely different from the yeast TRAPPII structurally. ..
  47. Bodrikov V, Solis G, Stuermer C. Prion protein promotes growth cone development through reggie/flotillin-dependent N-cadherin trafficking. J Neurosci. 2011;31:18013-25 pubmed publisher
    ..This implies that PrP functions in reggie-mediated signaling and cargo trafficking, thus promoting growth cone complexity and vitality and thereby growth cone elongation. ..
  48. Punzo F, Bertoli Avella A, Scianguetta S, Della Ragione F, Casale M, Ronzoni L, et al. Congenital dyserythropoietic anemia type II: molecular analysis and expression of the SEC23B gene. Orphanet J Rare Dis. 2011;6:89 pubmed publisher
    ..Finally, we demonstrate that decreased Sec23B protein levels in erythroid precursors correlate with down-regulation of the SEC23B mRNA transcript. ..
  49. Loftus A, Hsieh V, Parthasarathy R. Modulation of membrane rigidity by the human vesicle trafficking proteins Sar1A and Sar1B. Biochem Biophys Res Commun. 2012;426:585-9 pubmed publisher
    ..At high concentrations, we find increased bending rigidity and decreased protein mobility. These features imply a model in which protein clustering governs membrane mechanical properties. ..
  50. Kondo Okamoto N, Noda N, Suzuki S, Nakatogawa H, Takahashi I, Matsunami M, et al. Autophagy-related protein 32 acts as autophagic degron and directly initiates mitophagy. J Biol Chem. 2012;287:10631-8 pubmed publisher
    ..Taken together, our data implicate Atg32 as a bipartite platform recruiting Atg8 and Atg11 to the mitochondrial surface and forming an initiator complex crucial for mitophagy. ..
  51. Wang S, Wu X, Pan T, Song W, Wang Y, Zhang F, et al. Viperin inhibits hepatitis C virus replication by interfering with binding of NS5A to host protein hVAP-33. J Gen Virol. 2012;93:83-92 pubmed publisher
    ..In summary, these findings suggest a novel mechanism by which viperin inhibits HCV replication, possibly through binding to host protein hVAP-33 and interfering with its interaction with NS5A...
  52. Heider M, Munson M. Exorcising the exocyst complex. Traffic. 2012;13:898-907 pubmed publisher
    ..In this review, we discuss current knowledge of exocyst architecture, assembly, regulation and its roles in a variety of cellular trafficking pathways. ..
  53. Verstraeten A, Wauters E, Crosiers D, Meeus B, Corsmit E, Elinck E, et al. Contribution of VPS35 genetic variability to LBD in the Flanders-Belgian population. Neurobiol Aging. 2012;33:1844.e11-3 pubmed publisher
    ..Hence, our data do not support a major role for VPS35 variations in the genetic etiology of Lewy body disorders in the Flanders-Belgian population. ..