immobilized proteins


Summary: Proteins that are chemically bound to a substrate material which renders their location fixed. The immobilization of proteins allows their use in chemical reactions without being diluted by solvent.

Top Publications

  1. Wong L, Khan F, Micklefield J. Selective covalent protein immobilization: strategies and applications. Chem Rev. 2009;109:4025-53 pubmed publisher
  2. Früh V, IJzerman A, Siegal G. How to catch a membrane protein in action: a review of functional membrane protein immobilization strategies and their applications. Chem Rev. 2011;111:640-56 pubmed publisher
  3. Steinhagen M, Holland Nell K, Meldal M, Beck Sickinger A. Simultaneous "one pot" expressed protein ligation and CuI-catalyzed azide/alkyne cycloaddition for protein immobilization. Chembiochem. 2011;12:2426-30 pubmed publisher
  4. Qasem Abdullah H, Perach M, Livnat Levanon N, Lewinson O. ATP binding and hydrolysis disrupt the high-affinity interaction between the heme ABC transporter HmuUV and its cognate substrate-binding protein. J Biol Chem. 2017;292:14617-14624 pubmed publisher
    ..Subsequent ATP hydrolysis leads to substrate delivery to the cytoplasm, release of the SBP, and resetting of the system. We propose that other Type II ABC transporters likely share the fundamentals of this mechanism. ..
  5. Lowndes M, Junyent S, Habib S. Constructing cellular niche properties by localized presentation of Wnt proteins on synthetic surfaces. Nat Protoc. 2017;12:1498-1512 pubmed publisher
    ..Therefore, this method can also be incorporated into tissue engineering platforms in which depletion of the stem cell pool restricts the complexity and maturity of the tissue developed. ..
  6. Blanco Toribio A, Lacadena J, Nuñez Prado N, Álvarez Cienfuegos A, Villate M, Compte M, et al. Efficient production of single-chain fragment variable-based N-terminal trimerbodies in Pichia pastoris. Microb Cell Fact. 2014;13:116 pubmed publisher
    ..pastoris is about 20-fold higher than in human cells. P. pastoris is an efficient expression system for multivalent trimerbody molecules, suitable for their commercial production. ..
  7. Carlson S, Soulette C, Yang Z, Elias J, Brooks A, Gozani O. RBM25 is a global splicing factor promoting inclusion of alternatively spliced exons and is itself regulated by lysine mono-methylation. J Biol Chem. 2017;292:13381-13390 pubmed publisher
    ..Together, our findings uncover a pivotal role for RBM25 as an essential regulator of alternative splicing and reveal a new potential mechanism for regulation of pre-mRNA splicing by lysine methylation of a splicing factor. ..
  8. Feinberg H, Jégouzo S, Rex M, Drickamer K, Weis W, Taylor M. Mechanism of pathogen recognition by human dectin-2. J Biol Chem. 2017;292:13402-13414 pubmed publisher
  9. Lewis J, Elder P. Monoclonal antibodies to the reactive centre loop (RCL) of human corticosteroid-binding globulin (CBG) can protect against proteolytic cleavage. J Steroid Biochem Mol Biol. 2017;171:247-253 pubmed publisher
    ..These findings may provide a basis and rationale for the concept of the use of RCL antibodies as therapeutic agents to effectively increase the proportion of intact CBG in circulation which may be of benefit in acute inflammation. ..

More Information


  1. Williams C, Dougherty M, Makaroff K, Stapleton J, Konkolewicz D, Berberich J, et al. Strategies for Biophysical Characterization of Protein-Polymer Conjugates. Methods Enzymol. 2017;590:93-114 pubmed publisher
    ..Descriptions of the assays include a discussion of advantages and disadvantages alongside protocol details and approaches to data analysis. ..
  2. Ebisu K, Tateno H, Kuroiwa H, Kawakami K, Ikeuchi M, Hirabayashi J, et al. N-terminal specific point-immobilization of active proteins by the one-pot NEXT-A method. Chembiochem. 2009;10:2460-4 pubmed publisher
  3. Iwase K, Tanaka M, Hirose K, Uyeda T, Honda H. Acceleration of the sliding movement of actin filaments with the use of a non-motile mutant myosin in in vitro motility assays driven by skeletal muscle heavy meromyosin. PLoS ONE. 2017;12:e0181171 pubmed publisher
    ..We found that the velocity of actin filaments sliding on skeletal muscle myosin molecules increased in the presence of a non-motile G680V mutant myosin motor. ..
  4. Raghuwanshi Y, Etayash H, Soudy R, Paiva I, Lavasanifar A, Kaur K. Proteolytically Stable Cyclic Decapeptide for Breast Cancer Cell Targeting. J Med Chem. 2017;60:4893-4903 pubmed publisher
    ..The study highlights the discovery of a novel proteolytically stable cyclic peptide that can be used for targeted drug delivery or for enumerating circulating breast tumor cells. ..
  5. Yang S, Hu Y, Sokoll L, Zhang H. Simultaneous quantification of N- and O-glycans using a solid-phase method. Nat Protoc. 2017;12:1229-1244 pubmed publisher
    ..The GIG protocol takes ∼3 d. ..
  6. Singh N, Habicht K, Moaddel R, Shimmo R. Development and characterization of mitochondrial membrane affinity chromatography columns derived from skeletal muscle and platelets for the study of mitochondrial transmembrane proteins. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1055-1056:144-148 pubmed publisher
    ..001), similar to previously reported values. Additionally, the Michaelis-Menten constant (Km) for ADP was found to be 1525±461?M based on the on column dose-dependent increase in ATP production. ..
  7. Xing Y, Feng X, Zhang L, Hou J, Han G, Chen Z. A sensitive and selective electrochemical biosensor for the determination of beta-amyloid oligomer by inhibiting the peptide-triggered in situ assembly of silver nanoparticles. Int J Nanomedicine. 2017;12:3171-3179 pubmed publisher
    ..The detection limit of the method was found to be 6 pM. Furthermore, the amenability of this method for the analysis of A? oligomer in serum and artificial cerebrospinal fluid (aCSF) samples was demonstrated. ..
  8. Suthiwangcharoen N, Nagarajan R. Nanoarmoring of Proteins by Conjugation to Block Copolymer Micelles. Methods Enzymol. 2017;590:277-304 pubmed publisher
    ..These studies document a rational approach to armor the protein by conjugation with a block copolymer micelle, as a general approach. ..
  9. Xu J, Medina Rangel P, Haupt K, Tse Sum Bui B. Guide to the Preparation of Molecularly Imprinted Polymer Nanoparticles for Protein Recognition by Solid-Phase Synthesis. Methods Enzymol. 2017;590:115-141 pubmed publisher
    ..Moreover, these water-compatible polymers, targeting different domains of the enzyme, can also function as protective agents (armor), hence preventing the target proteins from denaturation by heat or pH. ..
  10. Horák D, Hlídková H, Kit Y, Antonyuk V, Myronovsky S, Stoika R. Magnetic poly(2-hydroxyethyl methacrylate) microspheres for affinity purification of monospecific anti-p46 kDa/Myo1C antibodies for early diagnosis of multiple sclerosis patients. Biosci Rep. 2017;37: pubmed publisher
    ..The newly developed mgt.PHEMA microspheres conjugated with a potential disease biomarker, p46/Myo1C protein, are thus a promising tool for affinity purification of antibodies, which can improve diagnosis and treatment of MS patients. ..
  11. Cheng Y, Ramos D, Lee P, Liang D, Yu X, Kumbar S. Collagen functionalized bioactive nanofiber matrices for osteogenic differentiation of mesenchymal stem cells: bone tissue engineering. J Biomed Nanotechnol. 2014;10:287-98 pubmed
    ..These fiber matrices and modification techniques could be extended to other scaffold systems for tissue engineering applications. ..
  12. Wang Y, van Assen A, Reis C, Setroikromo R, van Merkerk R, Boersma Y, et al. Novel RANKL DE-loop mutants antagonize RANK-mediated osteoclastogenesis. FEBS J. 2017;284:2501-2512 pubmed publisher
    ..The generation of RANK-antagonists is a promising strategy for the exploration of new therapeutics against osteoporosis. ..
  13. Kerr H, Wong E, Makou E, Yang Y, Marchbank K, Kavanagh D, et al. Disease-linked mutations in factor H reveal pivotal role of cofactor activity in self-surface-selective regulation of complement activation. J Biol Chem. 2017;292:13345-13360 pubmed publisher
    ..We conclude that CCPs 19 and 20 are critical for efficient CA on self-surfaces but less important for DAA. Exposing CCPs 19 and 20 with PspCN and thus enhancing CA on self-surfaces may reverse deficiencies of some CFH variants. ..
  14. Peters R, Gutierrez Rivera L, Dew S, Stepanova M. Surface enhanced Raman spectroscopy detection of biomolecules using EBL fabricated nanostructured substrates. J Vis Exp. 2015;: pubmed publisher
    ..The capabilities of SERS to detect vibrational modes from surface-immobilized proteins, as well as to capture the protein-ligand and aptamer-ligand binding are demonstrated...
  15. Luo J, Li C, Xu T, Liu W, Ba X, Wang X, et al. PI3K is involved in ?1 integrin clustering by PSGL-1 and promotes ?1 integrin-mediated Jurkat cell adhesion to fibronectin. Mol Cell Biochem. 2014;385:287-95 pubmed
    ..Taken together, we found a novel signal pathway, PSGL-1-PI3K-?1 integrin, demonstrating the cooperation between initial adhesion and subsequent arrest and stable adhesion...
  16. Boyle A, Roddick L, Bhakta V, Lambourne M, Junop M, Liaw P, et al. The complete N-terminal extension of heparin cofactor II is required for maximal effectiveness as a thrombin exosite 1 ligand. BMC Biochem. 2013;14:6 pubmed publisher
    ..They further suggest that, in isolated or transferred form, the entire HCII 1-75 region is required to ensure maximal binding of thrombin exosite 1. ..
  17. Shi B, Deng Y, Zhao P, Li X. Selecting a DNA-Encoded Chemical Library against Non-immobilized Proteins Using a "Ligate-Cross-Link-Purify" Strategy. Bioconjug Chem. 2017;28:2293-2301 pubmed publisher
    ..In practice, DELs are usually selected against purified and immobilized proteins using the typical "bind-wash-elute" protocol; however, selection methods compatible with non-immobilized ..