phosvitin

Summary

Summary: An egg yolk phosphoglycoprotein which contains about 90% of the yolk protein phosphorus. It is synthesized in the liver of the hen and transferred to the developing oocyte, where it is bound to lipoproteins within the yolk granules.

Top Publications

  1. Jung S, Kim D, Son J, Nam K, Ahn D, Jo C. The functional property of egg yolk phosvitin as a melanogenesis inhibitor. Food Chem. 2012;135:993-8 pubmed publisher
    b>Phosvitin is a phosphoglycoprotein present in egg yolk. More than half of the amino acids in phosvitin molecule are serine, of which >90% are phosphorylated. Therefore, phosvitin has a strong metal binding capability...
  2. Goulas A, Triplett E, Taborsky G. Oligophosphopeptides of varied structural complexity derived from the egg phosphoprotein, phosvitin. J Protein Chem. 1996;15:1-9 pubmed
    ..The phosphorylated residues tend to occur in uninterrupted runs of as many as 28 phosphoserines (as in Xenopus phosvitin)...
  3. Jiang B, Mine Y. Phosphopeptides derived from hen egg yolk phosvitin: effect of molecular size on the calcium-binding properties. Biosci Biotechnol Biochem. 2001;65:1187-90 pubmed
    Two different phosphopeptide (PPP) fragments derived from partially dephosphorylated hen egg yolk phosvitin were prepared by tryptic digestion, and their Ca2+ binding property compared with that of commercial casein phosphopeptides (CPP)...
  4. Raag R, Appelt K, Xuong N, Banaszak L. Structure of the lamprey yolk lipid-protein complex lipovitellin-phosvitin at 2.8 A resolution. J Mol Biol. 1988;200:553-69 pubmed
    The X-ray crystallographic structure of the lipid-protein complex lipovitellin-phosvitin has been determined with the multiple isomorphous replacement method using four heavy-atom derivatives...
  5. Sanchez Ruiz J, Martinez Carrion M. A Fourier-transform infrared spectroscopic study of the phosphoserine residues in hen egg phosvitin and ovalbumin. Biochemistry. 1988;27:3338-42 pubmed
    A Fourier-transform infrared spectroscopic study of hen egg phosvitin and ovalbumin has been carried out. Bands arising from monoanionic and dianionic phosphate monoester [Shimanouchi, T., Tsuboi, M., & Kyogoku, Y. (1964) Adv. Chem...
  6. Finn R. Vertebrate yolk complexes and the functional implications of phosvitins and other subdomains in vitellogenins. Biol Reprod. 2007;76:926-35 pubmed
    ..The serine-rich phosvitin (Pv) domains are linearly related to the molecular masses of the lipovitellin heavy chain...
  7. Grogan J, Shirazi A, Taborsky G. Phosphorus nuclear magnetic resonance of diverse phosvitin species. Comp Biochem Physiol B. 1990;96:655-63 pubmed
    ..phosphorus resonances provides the basis for further investigation of the particular molecular environments of phosvitin-bound phosphoryl groups and their involvement in the diverse binding modes for metal complex formation by ..
  8. Li B, Raff J, Barkleit A, Bernhard G, Foerstendorf H. Complexation of U(VI) with highly phosphorylated protein, phosvitin A vibrational spectroscopic approach. J Inorg Biochem. 2010;104:718-25 pubmed publisher
    The complexation of uranium(VI) to variant functional groups of the highly phosphorylated protein phosvitin in aqueous solution was investigated by attenuated total reflection Fourier transform infrared (ATR FT-IR) spectroscopy...
  9. Lim E, Teo B, Lam T, Ding J. Sequence analysis of a fish vitellogenin cDNA with a large phosvitin domain. Gene. 2001;277:175-86 pubmed
    ..This cDNA has the largest phosvitin (PV) domain amongst piscine Vtgs, being comparable to those of lamprey, Xenopus, and chicken...

More Information

Publications62

  1. Sattar Khan M, Nakamura S, Ogawa M, Akita E, Azakami H, Kato A. Bactericidal action of egg yolk phosvitin against Escherichia coliunder thermal stress. J Agric Food Chem. 2000;48:1503-6 pubmed
    Chicken egg yolk phosvitin showed a remarkable antibacterial effect against Escherichia coli under thermal stress at 50 degrees C. E. coli cells (10(6)/mL) completely disappeared in 1 mL of L-broth coexisting with 0...
  2. Wang H, Yan T, Tan J, Gong Z. A zebrafish vitellogenin gene (vg3) encodes a novel vitellogenin without a phosvitin domain and may represent a primitive vertebrate vitellogenin gene. Gene. 2000;256:303-10 pubmed
    ..Interestingly, the predicted vitellogenin has no polyserine phosvitin domain. The lack of the phosvitin domain was confirmed by isolation and sequencing of the vg3 genomic region...
  3. Taborsky G. On the interaction of phosvitins with ferric ion: solubility of the Fe(III)-phosphoprotein complex under acidic conditions is a function of the iron/phosphate ratio and the degree of phosvitin phosphorylation. J Inorg Biochem. 1991;44:65-77 pubmed
    ..5, precipitation becomes maximal. At Fe/P ratios above 0.5, the Fe(III)-phosvitin complex becomes increasingly soluble. At ratios above 2, solubility is essentially fully restored...
  4. Walsh B, Barnett D, Burley R, Elliott C, Hill D, Howden M. New allergens from hen's egg white and egg yolk. In vitro study of ovomucin, apovitellenin I and VI, and phosvitin. Int Arch Allergy Appl Immunol. 1988;87:81-6 pubmed
    Three hen egg yolk proteins, apovitellenins I and VI and phosvitin, and one egg white protein, ovomucin, were purified and tested for their ability to bind IgE in the sera of patients hypersensitive to egg...
  5. Wang S, Wang Y, Ma J, Ding Y, Zhang S. Phosvitin plays a critical role in the immunity of zebrafish embryos via acting as a pattern recognition receptor and an antimicrobial effector. J Biol Chem. 2011;286:22653-64 pubmed publisher
    ..embryo both displayed antimicrobial activity against microbes, including pathogenic Aeromonas hydrophila, and phosvitin (Pv), a nutritional protein abundant in eggs, was related to this antimicrobial activity...
  6. Sawaguchi S, Ohkubo N, Matsubara T. Identification of two forms of vitellogenin-derived phosvitin and elucidation of their fate and roles during oocyte maturation in the barfin flounder, Verasper moseri. Zoolog Sci. 2006;23:1021-9 pubmed
    ..This indicates that the Pvs in the barfin flounder, a marine teleost spawning its eggs in seawater, do not play a role in the transport and deposition of calcium and magnesium into oocytes. ..
  7. Belhomme C, David Briand E, Guérin Dubiard C, Vié V, Anton M. Phosvitin-calcium aggregation and organization at the air-water interface. Colloids Surf B Biointerfaces. 2008;63:12-20 pubmed
    b>Phosvitin, an egg yolk protein constituted by 50% of phosphorylated serines, presents good emulsifying properties whereas its interfacial properties are not yet clearly elucidated and remain object of discussion...
  8. Romek M, Kilarski W. The lipoprotein crystals in yolk platelets of a shark, Squalus ocanthias (Selachii). Folia Histochem Cytobiol. 1993;31:139-45 pubmed
    Lipovitellin-phosvitin crystals in yolk platelets from the egg of a shark, Squalus ocanthias (Selachii), were studied by the selected area electron diffraction method...
  9. Richards M. Trace mineral metabolism in the avian embryo. Poult Sci. 1997;76:152-64 pubmed
    ..Lipovitellin and phosvitin, derived from intraoocytic proteolytic processing of vitellogenin, are also trace mineral binding proteins that ..
  10. Ishikawa S, Yano Y, Arihara K, Itoh M. Egg yolk phosvitin inhibits hydroxyl radical formation from the fenton reaction. Biosci Biotechnol Biochem. 2004;68:1324-31 pubmed
    b>Phosvitin, a phosphoprotein known as an iron-carrier in egg yolk, binds almost all the yolk iron. In this study, we investigated the effect of phosvitin on Fe(II)-catalyzed hydroxyl radical ((...
  11. Li H, Roux S. Purification and characterization of a casein kinase 2-type protein kinase from pea nuclei. Plant Physiol. 1992;99:686-92 pubmed
    ..The pea kinase can use casein and phosvitin as artificial substrates, phosphorylating both the serine and threonine residues of casein...
  12. Ding Y, Liu X, Bu L, Li H, Zhang S. Antimicrobial-immunomodulatory activities of zebrafish phosvitin-derived peptide Pt5. Peptides. 2012;37:309-13 pubmed publisher
    A phosvitin (Pv)-derived peptide, Pt5, which consists of the C-terminal 55 residues of Pv in zebrafish, has been shown to function as an antimicrobial agent capable of killing microbes in vitro...
  13. Walsh B, Hill D, Macoun P, Cairns D, Howden M. Detection of four distinct groups of hen egg allergens binding IgE in the sera of children with egg allergy. Allergol Immunopathol (Madr). 2005;33:183-91 pubmed
    ..fourth set contained the egg white protein ovotransferrin and the egg yolk proteins apovitellenins I and VI and phosvitin. The existence of patient groups may explain why various workers have reported different allergens to be ..
  14. Maheswari S, Ramadoss C, Krishnaswamy P. Inhibition of Fe(II) catalyzed linoleic acid oxidation and DNA damage by phosvitin. Mol Cell Biochem. 1997;177:47-51 pubmed
    The oxidation of linoleic acid catalyzed by Fe(II) is strongly inhibited by phosvitin, while chelation with EDTA, NTA or deferoxamine produced only partial inhibition...
  15. Naderi N, Doyen A, House J, Pouliot Y. The use of high hydrostatic pressure to generate folate-enriched extracts from the granule fraction of hen's egg yolk. Food Chem. 2017;232:253-262 pubmed publisher
    ..Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis revealed the localization of phosvitin in the plasma fraction, which correlated with higher folate concentrations...
  16. Maragoudakis M, Hankin H. Partial purification and properties of a cyclic 3',5'-AMP-independent protein kinase from rat liver. Biochim Biophys Acta. 1977;480:122-36 pubmed
    ..from the enzyme activity; this dissociation had no apparent effect on kinase activity with casein or phosvitin as substrates, or on the apparent molecular weight of the enzyme (approx. 158,000). 3...
  17. Sugimoto I, Hashimoto E. Modulation of protein phosphorylation by Mr 25,000 protein partially overlapping phosvitin and lipovitellin 2 in Xenopus laevis vitellogenin B1 protein. Protein J. 2006;25:109-15 pubmed
    ..These results suggest that pp25 may have a role as an inhibitory modulator of some protein phosphorylation in Xenopus oocytes and embryos. ..
  18. Dolińska B, Łopata K, Mikulska A, Leszczyńska L, Ryszka F. Influence of phosvitin and calcium gluconate concentration on permeation and intestinal absorption of calcium ions. Biol Trace Elem Res. 2012;147:374-7 pubmed publisher
    The effect of egg yolk phosvitin on the permeation and absorption of calcium was investigated in vitro in relation to calcium gluconate concentration...
  19. Hidaka S, Okamoto Y, Abe K, Miyazaki K. Effects of indium and iron ions on in vitro calcium phosphate precipitation and crystallinity. J Biomed Mater Res. 1996;31:11-8 pubmed
    ..of indium and ferric ions on the calcium phosphate precipitation in the absence and presence of an inhibitor (phosvitin, a phosphoprotein purified from egg yolks)...
  20. Jiang B, Mine Y. Preparation of novel functional oligophosphopeptides from hen egg yolk phosvitin. J Agric Food Chem. 2000;48:990-4 pubmed
    Novel hen egg phosvitin phosphopeptides (PPP) with molecular masses of 1-3 kDa were prepared from tryptic hydrolysis following partial alkaline dephosphorylation. The phosvitin treated with various NaOH concentrations (0.05-0...
  21. Martin R, Door R, Breitig D. High-resolution imaging of protein phosphorylation in squid axons and synapse by electron energy loss spectroscopy. J Histochem Cytochem. 1993;41:1133-9 pubmed
    ..Our method may be advantageous for analysis with high elemental and spatial resolution of the phosphorylation state of cytoskeletal protein molecules in situ. ..
  22. Stigare J, Kovacs J, Buddelmeijer N, Egyhazi E. A novel nuclear 42-kDa casein kinase identified in Chironomus tentans. FEBS Lett. 1992;314:327-30 pubmed
    ..The protein kinase promotes phosphorylation of casein and phosvitin, using either ATP or GTP as phosphate donors, and undergoes autophosphorylation...
  23. Nardelli D, van het Schip F, Gerber Huber S, Haefliger J, Gruber M, Ab G, et al. Comparison of the organization and fine structure of a chicken and a Xenopus laevis vitellogenin gene. J Biol Chem. 1987;262:15377-85 pubmed
    ..In contrast, one internal region is quite divergent. Part of this region encodes phosvitin, which appears to have evolved rapidly by both point mutations and duplications of serines or short other amino ..
  24. Liu J, Czernick D, Lin S, Alasmari A, Serge D, Salih E. Novel bioactivity of phosvitin in connective tissue and bone organogenesis revealed by live calvarial bone organ culture models. Dev Biol. 2013;381:256-75 pubmed publisher
    Egg yolk phosvitin is one of the most highly phosphorylated extracellular matrix proteins known in nature with unique physico-chemical properties deemed to be critical during ex-vivo egg embryo development...
  25. Saito T, Arsenault A, Yamauchi M, Kuboki Y, Crenshaw M. Mineral induction by immobilized phosphoproteins. Bone. 1997;21:305-11 pubmed
    ..Bovine dentin phosphophoryn or phosvitin from egg yolk were immobilized on reconstituted skin type I collagen fibrils by adsorption or by covalent cross-..
  26. Hehn B, Young A, Pelech S, Sanghera J, Shah R. Developmental alterations in casein kinase 2 activity during the morphogenesis of quail secondary palate. Anat Rec. 1997;247:102-8 pubmed
    ..Fractionation of palate extract using a Mono-Q column revealed the presence of a major peak of phosvitin phosphotransferase activity which eluted with 0.5 M NaCl...
  27. Duan X, Li M, Ma H, Xu X, Jin Z, Liu X. Physicochemical properties and antioxidant potential of phosvitin-resveratrol complexes in emulsion system. Food Chem. 2016;206:102-9 pubmed publisher
    Egg yolk phosvitin is the most highly phosphorylated protein found in the nature. The physicochemical properties of phosvitin-resveratrol complexes and their synergistic antioxidant activities in microemulsions were investigated...
  28. Nieuw Amerongen A, Oderkerk C, Veerman E. Adsorption to hydroxyapatite of partially deglycosylated human salivary mucins in competition with phosvitin and phytate. Biol Chem Hoppe Seyler. 1991;372:585-91 pubmed
    The effect of phosvitin and phytate on the binding of native as well as partially deglycosylated human whole salivary mucins (HWSM) to hydroxyapatite was studied...
  29. Sugawara K, Yugami A, Kadoya T. Voltammetric sensing of phosphoproteins using a gallium(III) acetylacetonate-modified carbon paste electrode. Anal Sci. 2012;28:251-5 pubmed
    ..When phosvitin and hexaammine ruthenium(III) ions were incubated in 0.1 M acetate buffer (pH 3...
  30. Ann K, Nelson D. A nucleoside diphosphate kinase from Paramecium tetraurelia with protein kinase activity. J Eukaryot Microbiol. 1996;43:365-72 pubmed
    ..The enzyme also catalyzed acid-stable phosphorylation of casein and phosvitin. This protein kinase activity is distinct from the histidine phosphorylation that is part of the NDP kinase ..
  31. Angiolillo A, Panara F, Piccinini G, Gianfranceschi G. Isolation and partial characterization of a protein kinase NII from wheat germ chromatin. Mol Biol Rep. 1991;15:39-43 pubmed
    ..The enzyme, which uses both ATP and GTP as phosphoryl donors, catalyzes the phosphorylation of casein, phosvitin and E. coli RNA polymerase, but not of histone proteins...
  32. Stifani S, Nimpf J, Schneider W. Vitellogenesis in Xenopus laevis and chicken: cognate ligands and oocyte receptors. The binding site for vitellogenin is located on lipovitellin I. J Biol Chem. 1990;265:882-8 pubmed
    ..AB, G., and Wahli, W. (1987) J. Biol. Chem. 262, 15377-15383), it appears that conservation of key structural elements required for efficient vitellogenesis extends from the ligands to their receptors on the oocyte plasma membrane. ..
  33. Katayama S, Ishikawa S, Fan M, Mine Y. Oligophosphopeptides derived from egg yolk phosvitin up-regulate gamma-glutamylcysteine synthetase and antioxidant enzymes against oxidative stress in Caco-2 cells. J Agric Food Chem. 2007;55:2829-35 pubmed
    Previously, we have found phosphopeptides (PPPs) from hen egg yolk phosvitin possess a potent antioxidative activity against oxidative stress in human intestinal epithelial cells, Caco-2...
  34. Sun J, Liu Y, Su Y, Xia W, Yang Y. Highly efficient enrichment of phosvitin phosphopeptides by novel magnetic carboxymethyl chitosan nanoparticles decorated with Fe (III) ions. J Chromatogr B Analyt Technol Biomed Life Sci. 2013;915-916:33-8 pubmed publisher
    ..chitosan nanoparticles (abbreviated as Fe(3)O(4) (PEG+CM-CTS) @ Fe (III)) were conveniently applied for phosvitin phosphopeptides (PPPs) enrichment for the first time...
  35. Kaur H, Sharma S, Khuller G. Partial purification and characterization of cyclic adenosine monophosphate dependent protein kinase from mycobacterium smegmatis. Biochem Mol Biol Int. 1996;38:141-51 pubmed
    ..The enzyme preferentially utilized phosvitin and Histones as exogenous phosphate acceptor...
  36. Yamane K, Yagai T, Nishimiya O, Sugawara R, Amano H, Fujita T, et al. Characterization of vitellogenin and its derived yolk proteins in cloudy catshark (Scyliorhinus torazame). Fish Physiol Biochem. 2013;39:373-90 pubmed publisher
    ..The apparent molecular weights of purified Vtg and putative Vtg-related YPs (lipovitellin: Lv, phosvitin: Pv) were determined by gel filtration and were ~560, >669 and ~58 kDa, respectively...
  37. Lee B, Lasanthi G, Jayathilaka P, Huang J, Gupta S. Immobilized metal affinity electrophoresis: a novel method of capturing phosphoproteins by electrophoresis. J Biomol Tech. 2008;19:106-8 pubmed
    ..In the case studies, phosphoproteins alpha-casein, beta-casein, and phosvitin are successfully extracted from a protein mixture by IMAEP.
  38. Ishikawa S, Tamaki S, Arihara K, Itoh M. Egg yolk protein and egg yolk phosvitin inhibit calcium, magnesium, and iron absorptions in rats. J Food Sci. 2007;72:S412-9 pubmed
    Egg yolk decreases the absorption of iron. The effects of egg yolk protein and egg yolk phosvitin on the absorption of calcium, magnesium, and iron were investigated by in vivo studies...
  39. Sawaguchi S, Kagawa H, Ohkubo N, Hiramatsu N, Sullivan C, Matsubara T. Molecular characterization of three forms of vitellogenin and their yolk protein products during oocyte growth and maturation in red seabream (Pagrus major), a marine teleost spawning pelagic eggs. Mol Reprod Dev. 2006;73:719-36 pubmed
    ..The third red seabream Vg was classified as a type-C or phosvitinless (Pvl) Vg due to its lack of a phosvitin (Pv) domain...
  40. Ishikawa S, Ohtsuki S, Tomita K, Arihara K, Itoh M. Protective effect of egg yolk phosvitin against ultraviolet- light-induced lipid peroxidation in the presence of iron ions. Biol Trace Elem Res. 2005;105:249-56 pubmed
    ..In this study, we evaluated the effect of egg yolk phosvitin on UV-light-induced oxidative stress...
  41. Hiramatsu N, Hara A. Relationship between vitellogenin and its related egg yolk proteins in Sakhalin taimen (Hucho perryi). Comp Biochem Physiol A Physiol. 1996;115:243-51 pubmed
    Vitellogenin (Vg) and its three egg yolk protein products, lipovitellin (Lv), phosvitin (Pv) and beta'-component, were isolated from mature female Sakhalin taimen (Hucho perryi)...
  42. Linde A, Lussi A, Crenshaw M. Mineral induction by immobilized polyanionic proteins. Calcif Tissue Int. 1989;44:286-95 pubmed
    ..protein of the osteocalcin type (Gla-protein), proteoglycan (PG), and phosphoprotein (PP-H), as well as phosvitin (PhV) and bovine serum albumin (BSA), were covalently linked to agarose beads...
  43. Tanabe H, Ogawa N, Hayashi K, Ito S, Tanabe Y. Genetic variants of phosvitin in egg yolk of the Japanese quail, Coturnix coturnix japonica. Anim Genet. 1989;20:287-94 pubmed
    b>Phosvitin polymorphism in egg yolk of the Japanese quail was found by horizontal polyacrylamide gradient gel electrophoresis. Six phenotypes of yolk phosvitin designated A, B, C, AB, AC, and BC were observed in a population of 281 birds...
  44. Wallace R, Carnevali O, Hollinger T. Preparation and rapid resolution of Xenopus phosvitins and phosvettes by high-performance liquid chromatography. J Chromatogr. 1990;519:75-86 pubmed
    ..Procedures were further developed to resolve the various entities present in the crude phosvitin/phosvette fraction by size-exclusion, anion-exchange, and hydrophobic interaction chromatography, using a ..
  45. Zhang X, Qiu N, Geng F, Ma M. Simply and effectively preparing high-purity phosvitin using polyethylene glycol and anion-exchange chromatography. J Sep Sci. 2011;34:3295-301 pubmed publisher
    An attempt was made to develop a new protocol for preparing phosvitin that could be easy scaled up using polyethylene glycol (PEG6000). Influence of PEG6000 concentration and pH of sample solution on phosvitin isolation was investigated...
  46. Byrne B, de Jong H, Fouchier R, Williams D, Gruber M, Ab G. Rudimentary phosvitin domain in a minor chicken vitellogenin gene. Biochemistry. 1989;28:2572-7 pubmed
    ..The major egg yolk phosphoprotein, phosvitin, is encoded by the analogous region in vitellogenin II...
  47. Zhang S, Wang Z, Wang H. Maternal immunity in fish. Dev Comp Immunol. 2013;39:72-8 pubmed publisher
    ..Recently, yolk proteins, phosvitin and lipovitellin, have been shown to be maternally-transferred factors, functioning in the defense of teleost ..
  48. Du C, Falini G, Fermani S, Abbott C, Moradian Oldak J. Supramolecular assembly of amelogenin nanospheres into birefringent microribbons. Science. 2005;307:1450-4 pubmed
  49. Gerber Huber S, Nardelli D, Haefliger J, Cooper D, Givel F, Germond J, et al. Precursor-product relationship between vitellogenin and the yolk proteins as derived from the complete sequence of a Xenopus vitellogenin gene. Nucleic Acids Res. 1987;15:4737-60 pubmed
    ..biochemical data obtained from the different yolk proteins allowed us to localize the cleavage products on the vitellogenin precursor as follows: NH2 - lipovitellin I - phosvitin (or phosvette II - phosvette I) - lipovitellin II - COOH.
  50. Paas Y, Fishelson Z. Shedding of tyrosine and serine/threonine ecto-protein kinases from human leukemic cells. Arch Biochem Biophys. 1995;316:780-8 pubmed
    ..We have found that in the presence of ATP and Mg2+, exogenously added substrates such as phosvitin and poly(Glu4-Tyr) are phosphorylated by intact K562 erythroleukemia, HL60 promyelocytic leukemia, and U937 ..
  51. Smyth E, Syme C, Blanch E, Hecht L, Vasak M, Barron L. Solution structure of native proteins with irregular folds from Raman optical activity. Biopolymers. 2001;58:138-51 pubmed
    Raman optical activity (ROA) spectra have been measured for the proteins hen phosvitin, yeast invertase, bovine alpha-casein, soybean Bowman-Birk protease inhibitor, and rabbit Cd(7)-metallothionein, all of which have irregular folds in ..
  52. Losso J, Bogumil R, Nakai S. Comparative studies of phosvitin from chicken and salmon egg yolk. Comp Biochem Physiol B. 1993;106:919-23 pubmed
    1. A method developed for the isolation of phosvitin from chicken egg yolk was successfully applied to the isolation of phosvitin from salmon eggs. 2. Salmon roe phosvitin is smaller in molecular size than chicken egg phosvitin. 3...
  53. Shinozaki T, Watanabe H, Arita S, Chigira M. Amino acid phosphatase activity of alkaline phosphatase. A possible role of protein phosphatase. Eur J Biochem. 1995;227:367-71 pubmed
    Alkaline phosphatase (ALP) hydrolyzed phosvitin and amino acid phosphates demonstrating nonisotropy at different pH...