octamer transcription factors


Summary: A family of POU domain factors that bind the octamer motif ATTTGCAT in enhancer and PROMOTER REGIONS to regulate GENE EXPRESSION.

Top Publications

  1. Passon D, Lee M, Rackham O, Stanley W, Sadowska A, Filipovska A, et al. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Proc Natl Acad Sci U S A. 2012;109:4846-50 pubmed publisher
    ..These results suggest that paraspeckles may potentially form through self-association of DBHS dimers into higher-order structures. ..
  2. Amelio A, Miraglia L, Conkright J, Mercer B, Batalov S, Cavett V, et al. A coactivator trap identifies NONO (p54nrb) as a component of the cAMP-signaling pathway. Proc Natl Acad Sci U S A. 2007;104:20314-9 pubmed
    ..These data demonstrate the utility of the coactivator trap by identification of a component of cAMP-mediated transcription. ..
  3. Clark J, Lu Y, Sidhar S, Parker C, Gill S, Smedley D, et al. Fusion of splicing factor genes PSF and NonO (p54nrb) to the TFE3 gene in papillary renal cell carcinoma. Oncogene. 1997;15:2233-9 pubmed
    ..These observations suggest the possibility of intriguing links between the processes of RNA splicing, DNA transcription and oncogenesis. ..
  4. Straub T, Grue P, Uhse A, Lisby M, Knudsen B, Tange T, et al. The RNA-splicing factor PSF/p54 controls DNA-topoisomerase I activity by a direct interaction. J Biol Chem. 1998;273:26261-4 pubmed
    ..A physiological role of this stimulatory mechanism seems feasible, because topoisomerase I and PSF showed a patched colocalization in A431 cell nuclei, which varied with cell cycle. ..
  5. Kaneko S, Rozenblatt Rosen O, Meyerson M, Manley J. The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination. Genes Dev. 2007;21:1779-89 pubmed
    ..Together, our data indicate that p54nrb/PSF functions in recruitment of XRN2 to facilitate pre-mRNA 3' processing and transcription termination. ..
  6. Kameoka S, Duque P, Konarska M. p54(nrb) associates with the 5' splice site within large transcription/splicing complexes. EMBO J. 2004;23:1782-91 pubmed
  7. Dong X, Yu C, Shynlova O, Challis J, Rennie P, Lye S. p54nrb is a transcriptional corepressor of the progesterone receptor that modulates transcription of the labor-associated gene, connexin 43 (Gja1). Mol Endocrinol. 2009;23:1147-60 pubmed publisher
    ..We conclude that p54nrb is a transcriptional corepressor of PR. Decreased expression of p54nrb at the time of labor may act to derepress PR-mediated inhibition on connexin 43 expression and contribute to the initiation of labor. ..
  8. Bond C, Fox A. Paraspeckles: nuclear bodies built on long noncoding RNA. J Cell Biol. 2009;186:637-44 pubmed publisher
    ..Given the large numbers of long noncoding transcripts currently being discovered through whole transcriptome analysis, paraspeckles may be a paradigm for a class of subnuclear bodies formed around long noncoding RNA...
  9. Xu J, Zhong N, Wang H, Elias J, Kim C, Woldman I, et al. The Parkinson's disease-associated DJ-1 protein is a transcriptional co-activator that protects against neuronal apoptosis. Hum Mol Genet. 2005;14:1231-41 pubmed
    ..Mutations that impair the transcriptional co-activator function of DJ-1 render dopaminergic neurons vulnerable to apoptosis and may contribute to the pathogenesis of PD. ..

More Information


  1. Fox A, Bond C, Lamond A. P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. Mol Biol Cell. 2005;16:5304-15 pubmed
    ..Thus paraspeckles are the sites where a subset of the total cellular pool of p54nrb is targeted in a RNA Polymerase II-dependent manner. ..
  2. Salton M, Lerenthal Y, Wang S, Chen D, Shiloh Y. Involvement of Matrin 3 and SFPQ/NONO in the DNA damage response. Cell Cycle. 2010;9:1568-76 pubmed
    ..Collectively the data suggest that SFPQ, NONO and MATR3 are involved in the early stage of the DSB response, setting the scene for DSB repair. ..
  3. Chen L, Carmichael G. Altered nuclear retention of mRNAs containing inverted repeats in human embryonic stem cells: functional role of a nuclear noncoding RNA. Mol Cell. 2009;35:467-78 pubmed publisher
    ..Taken together, these results assign a biological function to a large noncoding nuclear RNA in the regulation of mRNA export...
  4. Dong B, Horowitz D, Kobayashi R, Krainer A. Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6. Nucleic Acids Res. 1993;21:4085-92 pubmed
  5. Zhang Z, Carmichael G. The fate of dsRNA in the nucleus: a p54(nrb)-containing complex mediates the nuclear retention of promiscuously A-to-I edited RNAs. Cell. 2001;106:465-75 pubmed
    ..We provide evidence that one function of the complex identified here is to anchor hyperedited RNAs to the nuclear matrix, while allowing selectively edited mRNAs to be exported. ..
  6. Ito T, Watanabe H, Yamamichi N, Kondo S, Tando T, Haraguchi T, et al. Brm transactivates the telomerase reverse transcriptase (TERT) gene and modulates the splicing patterns of its transcripts in concert with p54(nrb). Biochem J. 2008;411:201-9 pubmed
  7. Dong X, Sweet J, Challis J, Brown T, Lye S. Transcriptional activity of androgen receptor is modulated by two RNA splicing factors, PSF and p54nrb. Mol Cell Biol. 2007;27:4863-75 pubmed
    ..These data demonstrated that PSF and p54nrb complex with AR and play a key role in modulating AR-mediated gene transcription. ..
  8. Chen L, DeCerbo J, Carmichael G. Alu element-mediated gene silencing. EMBO J. 2008;27:1694-705 pubmed publisher
    ..Taken together, these results support a novel role for Alu elements in human gene regulation. ..
  9. Schiffner S, Zimara N, Schmid R, Bosserhoff A. p54nrb is a new regulator of progression of malignant melanoma. Carcinogenesis. 2011;32:1176-82 pubmed publisher
    ..Our results demonstrate that p54(nrb) is highly expressed in malignant melanoma and, as a MIA target molecule, it seems to be involved in the development and progression of malignant melanoma. ..
  10. Li S, Kuhne W, Kulharya A, Hudson F, Ha K, Cao Z, et al. Involvement of p54(nrb), a PSF partner protein, in DNA double-strand break repair and radioresistance. Nucleic Acids Res. 2009;37:6746-53 pubmed publisher
    ..Together, results indicate that p54(nrb) contributes to rapid and accurate repair of DSBs in vivo in human cells and that the PSF.p54(nrb) complex may thus be a potential target for radiosensitizer development. ..
  11. Zhang C, Zhang M, Shen Y, Burks J, Li X, LeMaire S, et al. Role of NonO-histone interaction in TNFalpha-suppressed prolyl-4-hydroxylase alpha1. Biochim Biophys Acta. 2008;1783:1517-28 pubmed publisher
    ..Defining this cytokine-mediated regulatory pathway may provide novel molecular targets for therapeutic intervention in preventing plaque rupture and acute coronary occlusion. ..
  12. Bladen C, Udayakumar D, Takeda Y, Dynan W. Identification of the polypyrimidine tract binding protein-associated splicing factor.p54(nrb) complex as a candidate DNA double-strand break rejoining factor. J Biol Chem. 2005;280:5205-10 pubmed
    ..Based on structural comparison with related proteins, we propose a model where the four RNA recognition motif domains in the heteromeric PSF.p54(nrb) complex cooperate to align separate DNA molecules. ..
  13. Myojin R, Kuwahara S, Yasaki T, Matsunaga T, Sakurai T, Kimura M, et al. Expression and functional significance of mouse paraspeckle protein 1 on spermatogenesis. Biol Reprod. 2004;71:926-32 pubmed
    ..Therefore, we conclude that mPSP1 may regulate multiple phases of important nuclear events during spermatogenesis. ..
  14. Zolotukhin A, Michalowski D, Bear J, Smulevitch S, Traish A, Peng R, et al. PSF acts through the human immunodeficiency virus type 1 mRNA instability elements to regulate virus expression. Mol Cell Biol. 2003;23:6618-30 pubmed
    ..Thus, p54nrb and PSF have properties of key factors mediating INS function and likely define a novel mRNA regulatory pathway that is hijacked by HIV-1. ..
  15. Ishitani K, Yoshida T, Kitagawa H, Ohta H, Nozawa S, Kato S. p54nrb acts as a transcriptional coactivator for activation function 1 of the human androgen receptor. Biochem Biophys Res Commun. 2003;306:660-5 pubmed
    ..Our results suggest that p54(nrb) functions as a coactivator of AR that potentiates transcription, and presumably splicing as well. ..
  16. Peng R, Dye B, Pérez I, Barnard D, Thompson A, Patton J. PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 2002;8:1334-47 pubmed
    ..Sedimentation analyses show that both proteins associate with spliceosomes and with U4/U6.U5 tri-snPNP. ..
  17. Emili A, Shales M, McCracken S, Xie W, Tucker P, Kobayashi R, et al. Splicing and transcription-associated proteins PSF and p54nrb/nonO bind to the RNA polymerase II CTD. RNA. 2002;8:1102-11 pubmed
    ..PSF and p54nrb/NonO may therefore provide a direct physical link between the pol II CTD and pre-mRNA processing components, at both the initiation and elongation phases of transcription. ..
  18. Straub T, Knudsen B, Boege F. PSF/p54(nrb) stimulates "jumping" of DNA topoisomerase I between separate DNA helices. Biochemistry. 2000;39:7552-8 pubmed
    ..Thus, stimulation of topoisomerase I catalysis by PSF/p54(nrb) seems to be affected by mobilization of the enzyme. ..
  19. Peters U, Haberhausen G, Kostrzewa M, Nolte D, Muller U. AFX1 and p54nrb: fine mapping, genomic structure, and exclusion as candidate genes of X-linked dystonia parkinsonism. Hum Genet. 1997;100:569-72 pubmed
    ..AFX1 and p54nrb were excluded as candidates of DYT3 by sequencing of the exons and the flanking intronic sequences in an XDP patient and a control, and by Northern blot analysis. ..
  20. Basu A, Dong B, Krainer A, Howe C. The intracisternal A-particle proximal enhancer-binding protein activates transcription and is identical to the RNA- and DNA-binding protein p54nrb/NonO. Mol Cell Biol. 1997;17:677-86 pubmed
    ..Based on the close sequence relatedness of this protein to PSF, which is required for pre-mRNA splicing in vitro, we discuss the possibility that p54nrb has dual roles in transcription and splicing. ..
  21. Ferrai C, Naum Onganía G, Longobardi E, Palazzolo M, Disanza A, Diaz V, et al. Induction of HoxB transcription by retinoic acid requires actin polymerization. Mol Biol Cell. 2009;20:3543-51 pubmed publisher
    ..We conclude that inducible Hox genes are selectively sensitive to the inhibition of actin polymerization and that actin polymerization is required for the assembly of a transcription complex on the regulatory region of the Hox genes. ..
  22. McKinnell C, Mitchell R, Morris K, Anderson R, Kelnar C, Wallace W, et al. Perinatal germ cell development and differentiation in the male marmoset (Callithrix jacchus): similarities with the human and differences from the rat. Hum Reprod. 2013;28:886-96 pubmed publisher
    ..This work was supported by Grant G33253 from the Medical Research Council, UK. No external funding was sought and there are no competing interests. ..
  23. Venkatarama T, Lai F, Luo X, Zhou Y, Newman K, King M. Repression of zygotic gene expression in the Xenopus germline. Development. 2010;137:651-60 pubmed publisher
    ..Our results in a vertebrate system further support the concept that one strategy used repeatedly during evolution for preserving the germline is RNAPII repression. ..
  24. Yasujima T, Ohta K, Inoue K, Yuasa H. Characterization of human OCT1-mediated transport of DAPI as a fluorescent probe substrate. J Pharm Sci. 2011;100:4006-12 pubmed publisher
    ..Thus, DAPI can be an alternative probe substrate that enables fluorometric rapid assays of the functionality of hOCT1. ..
  25. Kukimoto I, Mori S, Sato H, Takeuchi T, Kanda T. Transcription factor human Skn-1a enhances replication of human papillomavirus DNA through the direct binding to two sites near the viral replication origin. FEBS J. 2008;275:3123-35 pubmed publisher
    ..The data strongly suggest that, through the binding to the two sites, human Skn-1a enhances HPV DNA replication. ..
  26. Cortes Y, Ojeda M, Araya D, Dueñas F, Fernandez M, Peralta O. Isolation and multilineage differentiation of bone marrow mesenchymal stem cells from abattoir-derived bovine fetuses. BMC Vet Res. 2013;9:133 pubmed publisher
    ..The simplicity of isolation and the differentiation potential suggest that bMSC from abattoir-derived fetal BM are appropriate candidate for investigating MSC biology and for eventual applications for regenerative therapy. ..
  27. Hirose T, Virnicchi G, Tanigawa A, Naganuma T, Li R, Kimura H, et al. NEAT1 long noncoding RNA regulates transcription via protein sequestration within subnuclear bodies. Mol Biol Cell. 2014;25:169-83 pubmed publisher
    ..These data further confirm that paraspeckles are stress-responsive nuclear bodies and provide a model in which induced NEAT1 controls target gene transcription by protein sequestration into paraspeckles. ..
  28. Shen W, Liang X, Sun H, De Hoyos C, Crooke S. Depletion of NEAT1 lncRNA attenuates nucleolar stress by releasing sequestered P54nrb and PSF to facilitate c-Myc translation. PLoS ONE. 2017;12:e0173494 pubmed publisher
    ..Together, our results suggest that NEAT1 depletion rescues CX5461-induced nucleolar stress through facilitating c-Myc translation by relocating P54nrb/PSF from nuclear paraspeckles to c-Myc mRNAs. ..
  29. Naghdi M, Tiraihi T, Namin S, Arabkheradmand J. Transdifferentiation of bone marrow stromal cells into cholinergic neuronal phenotype: a potential source for cell therapy in spinal cord injury. Cytotherapy. 2009;11:137-52 pubmed publisher
    ..The transdifferentiation of BMSC into a cholinergic phenotype is feasible for replacement therapy in spinal cord injury. ..
  30. Rosonina E, Ip J, Calarco J, Bakowski M, Emili A, McCracken S, et al. Role for PSF in mediating transcriptional activator-dependent stimulation of pre-mRNA processing in vivo. Mol Cell Biol. 2005;25:6734-46 pubmed
    ..Collectively, these results suggest that the recruitment of PSF to activated promoters and the pol II CTD provides a mechanism by which transcription and pre-mRNA processing are coordinated within the cell. ..
  31. Beck I, Muller M, Mentlein R, Sadowski T, Mueller M, Paus R, et al. Matrix metalloproteinase-19 expression in keratinocytes is repressed by transcription factors Tst-1 and Skn-1a: implications for keratinocyte differentiation. J Invest Dermatol. 2007;127:1107-14 pubmed
    ..Our results suggest that Tst-1 and Skn-1a regulate expression of MMPs in keratinocytes and effect both the expression and activation of these proteolytic enzymes. ..
  32. Stier S, Totzke G, Gruewald E, Neuhaus T, Fronhoffs S, Schoneborn S, et al. Identification of p54(nrb) and the 14-3-3 Protein HS1 as TNF-alpha-inducible genes related to cell cycle control and apoptosis in human arterial endothelial cells. J Biochem Mol Biol. 2005;38:447-56 pubmed
  33. Wu X, Yoo Y, Okuhama N, Tucker P, Liu G, Guan J. Regulation of RNA-polymerase-II-dependent transcription by N-WASP and its nuclear-binding partners. Nat Cell Biol. 2006;8:756-63 pubmed
    ..Based on these results, we propose a nuclear function for N-WASP in transcriptional regulation. ..
  34. Hallier M, Tavitian A, Moreau Gachelin F. The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb. J Biol Chem. 1996;271:11177-81 pubmed
    ..Altogether, these results raise the intriguing possibility that the functional interference observed between Spi-1/PU.1 and RNA-binding proteins might represent a novel mechanism in malignant erythropoiesis. ..
  35. Mito M, Kawaguchi T, Hirose T, Nakagawa S. Simultaneous multicolor detection of RNA and proteins using super-resolution microscopy. Methods. 2016;98:158-165 pubmed publisher
    ..Here, we describe multi-color fluorescent in situ hybridization techniques optimized for the simultaneous detection of RNA and proteins using super-resolution microscopy, namely structured illumination microscopy (SIM). ..
  36. Seino S, Shigeishi H, Hashikata M, Higashikawa K, Tobiume K, Uetsuki R, et al. CD44(high) /ALDH1(high) head and neck squamous cell carcinoma cells exhibit mesenchymal characteristics and GSK3β-dependent cancer stem cell properties. J Oral Pathol Med. 2016;45:180-8 pubmed publisher
    ..Our results show that GSK3β plays a major role in maintenance of stemness of CD44(high) /ALDH1(high) HNSCC cells. Additionally, they indicate a close relationship between CSC and mesenchymal characteristics in HNSCC. ..
  37. Ishiguro H, Uemura H, Fujinami K, Ikeda N, Ohta S, Kubota Y. 55 kDa nuclear matrix protein (nmt55) mRNA is expressed in human prostate cancer tissue and is associated with the androgen receptor. Int J Cancer. 2003;105:26-32 pubmed
    ..The PSA promoter was activated by nmt55 expression. These results suggest the possibility that nmt55 expression is related to hormone-dependency or -independence associated with the AR. ..
  38. Yamauchi T, Nakamura N, Hiramoto M, Yuri M, Yokota H, Naitou M, et al. Sepantronium bromide (YM155) induces disruption of the ILF3/p54(nrb) complex, which is required for survivin expression. Biochem Biophys Res Commun. 2012;425:711-6 pubmed publisher
    ..Thus, identification of molecular targets of YM155 in suppression of the survivin pathway, might lead to development of its use as a novel potential target in cancers. ..
  39. Poole R, Leith D, Docherty L, Shmela M, Gicquel C, Splitt M, et al. Beckwith-Wiedemann syndrome caused by maternally inherited mutation of an OCT-binding motif in the IGF2/H19-imprinting control region, ICR1. Eur J Hum Genet. 2012;20:240-3 pubmed publisher
    ..This is a second report of a point mutation causing ICR1 hypermethylation by altering an OCT-binding motif. The atypical growth phenotype of the brothers may be connected to the unusual underlying cause of their BWS. ..
  40. Fahrmayr C, Fromm M, Konig J. Hepatic OATP and OCT uptake transporters: their role for drug-drug interactions and pharmacogenetic aspects. Drug Metab Rev. 2010;42:380-401 pubmed publisher
  41. Grant M, Baron R, Macias A, Layne M, Perrella M, Rigby A. Netropsin improves survival from endotoxaemia by disrupting HMGA1 binding to the NOS2 promoter. Biochem J. 2009;418:103-12 pubmed publisher
  42. Roepcke S, Stahlberg S, Klein H, Schulz M, Theobald L, Gohlke S, et al. A tandem sequence motif acts as a distance-dependent enhancer in a set of genes involved in translation by binding the proteins NonO and SFPQ. BMC Genomics. 2011;12:624 pubmed publisher
  43. Kuhnert A, Schmidt U, Monajembashi S, Franke C, Schlott B, Grosse F, et al. Proteomic identification of PSF and p54(nrb) as TopBP1-interacting proteins. J Cell Biochem. 2012;113:1744-53 pubmed publisher
    ..Both p54(nrb) and PSF disappeared after 20 s while TopBP1 was retained at damage sites significantly longer suggesting different functions of the proteins during DSB recognition and repair. ..
  44. Roberts E, Hammond K, Traish A, Resing K, Ahn N. Identification of G2/M targets for the MAP kinase pathway by functional proteomics. Proteomics. 2006;6:4541-53 pubmed
  45. Chin A, Fong W, Goh L, Philp R, Oh S, Choo A. Identification of proteins from feeder conditioned medium that support human embryonic stem cells. J Biotechnol. 2007;130:320-8 pubmed
    ..Using proteomic tools, important proteins from CM that supports hESC culture have been identified, which when replaced with recombinant proteins, continue to support undifferentiated hESC growth in a feeder-free culture platform. ..
  46. Tantin D. Oct transcription factors in development and stem cells: insights and mechanisms. Development. 2013;140:2857-66 pubmed publisher
  47. Ohmoto M, Yamaguchi T, Yamashita J, Bachmanov A, Hirota J, Matsumoto I. Pou2f3/Skn-1a is necessary for the generation or differentiation of solitary chemosensory cells in the anterior nasal cavity. Biosci Biotechnol Biochem. 2013;77:2154-6 pubmed
    ..Here, we demonstrate that Pou2f3/Skn-1a, which is necessary for generation of sweet, umami, and bitter taste cells, is also necessary for the generation or differentiation of solitary chemosensory cells. ..
  48. Cabral A, Fischer D, Vermeij W, Backendorf C. Distinct functional interactions of human Skn-1 isoforms with Ese-1 during keratinocyte terminal differentiation. J Biol Chem. 2003;278:17792-9 pubmed
    ..Apparently, the human Skn-1 locus encodes multifunctional protein isotypes, subjected to biochemical cross-talk, which are likely to play a major role in the fine-tuning of keratinocyte terminal differentiation. ..
  49. Hung C, Lee Y, Chen D, Li C. Proteomic analysis of methylarginine-containing proteins in HeLa cells by two-dimensional gel electrophoresis and immunoblotting with a methylarginine-specific antibody. Protein J. 2009;28:139-47 pubmed publisher
    ..In this study we identified methylarginine-containing proteins in HeLa cells through proteomic approaches and the method is fast and robust for further applications. ..
  50. Sarkar C, Maitra A. Elucidation of regulatory mechanisms revealed by human promoter sequence analysis of genes co-expressed in forskolin-treated theca cells in PCOS. Arch Gynecol Obstet. 2013;287:477-85 pubmed publisher
    ..These factors might reveal a new insight into the complex pathogenesis of PCOS especially with respect to cAMP pathway. ..
  51. Schmid R, Meyer K, Spang R, Schittek B, Bosserhoff A. Melanoma inhibitory activity promotes melanoma development through activation of YBX1. Pigment Cell Melanoma Res. 2013;26:685-96 pubmed publisher
    ..In summary, we present for the first time effects of MIA on transcriptional regulation. Uncovering new potential downstream effectors working via activation of YBX1 supports the important role of MIA in melanoma. ..
  52. Buxade M, Morrice N, Krebs D, Proud C. The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha. J Biol Chem. 2008;283:57-65 pubmed
    ..These findings identify a novel Mnk substrate. They also suggest that the Mnk-catalyzed phosphorylation of PSF may regulate the fate of specific mRNAs by modulating their binding to PSF.p54(nrb). ..
  53. Richard M, Hikima J, Wilson M, Miller N, Cunningham C, Warr G. BOB.1 of the channel catfish, Ictalurus punctatus: not a transcriptional coactivator?. Mol Immunol. 2009;46:481-91 pubmed publisher
    ..Analysis of recombinant chimeric catfish and human BOB.1 proteins demonstrated that the failure to drive transcription was due to the lack of a functional activation domain within the catfish BOB.1. ..