mutl proteins


Summary: DNA repair proteins that include the bacterial MutL protein and its eukaryotic homologs. They consist of a conserved N-terminal region with weak ATPase activity, an endonuclease motif, and a C-terminal domain that forms MutL homodimers or heterodimers between MLH1 and the PMS1, MISMATCH REPAIR ENDONUCLEASE PMS2; or MLH3 proteins. These complexes function in DNA repair pathways, primarily DNA MISMATCH REPAIR, where MutL/MLH1 and the MUTS DNA MISMATCH-BINDING PROTEIN are targeted to damaged DNA.

Top Publications

  1. Villahermosa D, Christensen O, Knapp K, Fleck O. Schizosaccharomyces pombe MutS? and MutL? Maintain Stability of Tetra-Nucleotide Repeats and Msh3 of Hepta-Nucleotide Repeats. G3 (Bethesda). 2017;7:1463-1473 pubmed publisher
    ..We conclude that Msh3 has no obvious function in MMR in S. pombe, but contributes to DNA repeat stability in MMR-independent processes. ..
  2. Manhart C, Ni X, White M, Ortega J, Surtees J, Alani E. The mismatch repair and meiotic recombination endonuclease Mlh1-Mlh3 is activated by polymer formation and can cleave DNA substrates in trans. PLoS Biol. 2017;15:e2001164 pubmed publisher
    ..Together these observations argue that Mlh1-Mlh3 may not act like a canonical, RuvC-like Holliday junction resolvase and support a novel model in which Mlh1-Mlh3 is loaded onto DNA to form an activated polymer that cleaves DNA. ..
  3. Claeys Bouuaert C, Keeney S. Distinct DNA-binding surfaces in the ATPase and linker domains of MutL? determine its substrate specificities and exert separable functions in meiotic recombination and mismatch repair. PLoS Genet. 2017;13:e1006722 pubmed publisher
    ..Taken together, our results offer insights into the structure-function relationships of the MutL? complex and reveal unanticipated genetic relationships between components of the meiotic recombination machinery. ..
  4. Martins Pinheiro M, Oliveira A, Valencia A, Fernández Silva F, Silva L, Lopes Kulishev C, et al. Molecular characterization of Caulobacter crescentus mutator strains. Gene. 2017;626:251-257 pubmed publisher
    ..In spite of the limitations of using a single marker, possible reasons for the observed mutational bias are discussed in the light of the repertoire of DNA repair genes in this bacterium. ..
  5. Fukui K, Iino H, Baba S, Kumasaka T, Kuramitsu S, Yano T. Crystal structure and DNA-binding property of the ATPase domain of bacterial mismatch repair endonuclease MutL from Aquifex aeolicus. Biochim Biophys Acta Proteins Proteom. 2017;1865:1178-1187 pubmed publisher
    ..On the basis of the model structure and further experimental results, we concluded that the two separate DNA-binding sites in the full-length A. aeolicus MutL simultaneously bind a dsDNA molecule. ..
  6. Al Sweel N, Raghavan V, Dutta A, Ajith V, Di Vietro L, Khondakar N, et al. mlh3 mutations in baker's yeast alter meiotic recombination outcomes by increasing noncrossover events genome-wide. PLoS Genet. 2017;13:e1006974 pubmed publisher
    ..They also illustrate how defective meiotic components can alter the fate of meiotic recombination intermediates, providing new insights for how meiotic recombination pathways are regulated. ..
  7. Dahal B, Kadyrova L, Delfino K, Rogozin I, Gujar V, Lobachev K, et al. Involvement of DNA mismatch repair in the maintenance of heterochromatic DNA stability in Saccharomyces cerevisiae. PLoS Genet. 2017;13:e1007074 pubmed publisher