ku autoantigen

Summary

Summary: An ATP-dependent DNA HELICASE that preferentially binds SINGLE-STRANDED DNA. It is a heterodimer consisting of an 80 kDa subunit (XRCC5) and 70 kDa subunit (XRCC6) that functions with DNA LIGASE IV in the repair of DOUBLE-STRANDED DNA BREAKS and V(D)J RECOMBINATION.

Top Publications

  1. Giffin W, Torrance H, Rodda D, Préfontaine G, Pope L, Hache R. Sequence-specific DNA binding by Ku autoantigen and its effects on transcription. Nature. 1996;380:265-8 pubmed
    ..Recruitment of DNA-PK to DNA is by Ku autoantigen, a DNA-end-binding protein required for DNA-PK catalytic activity...
  2. Yang S, Nussenzweig A, Li L, Kim D, Ouyang H, Burgman P, et al. Modulation of thermal induction of hsp70 expression by Ku autoantigen or its individual subunits. Mol Cell Biol. 1996;16:3799-806 pubmed
    ..of heat shock response, we purified CHBF to apparent homogeneity and showed it to be identical to the Ku autoantigen, a heterodimer consisting of 70-kDa (Ku-70) and 86-kDa (Ku-80) polypeptides...
  3. Ochem A, Skopac D, Costa M, Rabilloud T, Vuillard L, Simoncsits A, et al. Functional properties of the separate subunits of human DNA helicase II/Ku autoantigen. J Biol Chem. 1997;272:29919-26 pubmed
    ..The three separate species (heterodimer, 70-kDa subunit, and 83-kDa subunit homodimer) all have ssDNA-dependent ATPase activity. ..
  4. Reddy S, Li B, Comai L. Processing of human telomeres by the Werner syndrome protein. Cell Cycle. 2010;9:3137-8 pubmed publisher
  5. Okumura K, Takagi S, Sakaguchi G, Naito K, Minoura Tada N, Kobayashi H, et al. Autoantigen Ku protein is involved in DNA binding proteins which recognize the U5 repressive element of human T-cell leukemia virus type I long terminal repeat. FEBS Lett. 1994;356:94-100 pubmed
    ..As Ku is known to be involved in transcriptional regulation, the specific interaction of Ku with U5RE raises intriguing possibilities for its function in HTLV-I basal transcriptional repression. ..
  6. Zhu S, Fisher L, Bessho T, Peng A. Protein phosphatase 1 and phosphatase 1 nuclear targeting subunit-dependent regulation of DNA-dependent protein kinase and non-homologous end joining. Nucleic Acids Res. 2017;45:10583-10594 pubmed publisher
    ..PNUTS associates with the DNA-PK complex and is required for DNA-PKcs phosphorylation at Ser-2056 and Thr-2609. Thus, PNUTS and PP1 together fine-tune the dynamic phosphorylation of DNA-PKcs after DNA damage to mediate NHEJ. ..
  7. Myler L, Gallardo I, Soniat M, Deshpande R, Gonzalez X, Kim Y, et al. Single-Molecule Imaging Reveals How Mre11-Rad50-Nbs1 Initiates DNA Break Repair. Mol Cell. 2017;67:891-898.e4 pubmed publisher
    ..Our results provide a mechanism for how MRN promotes homologous recombination on nucleosome-coated DNA. ..
  8. Morchikh M, Cribier A, Raffel R, Amraoui S, Cau J, Severac D, et al. HEXIM1 and NEAT1 Long Non-coding RNA Form a Multi-subunit Complex that Regulates DNA-Mediated Innate Immune Response. Mol Cell. 2017;67:387-399.e5 pubmed publisher
    ..Our study establishes the HDP-RNP as a key nuclear regulator of DNA-mediated activation of innate immune response through the cGAS-STING pathway. ..
  9. Wang J, Kang L, Song D, Liu L, Yang S, Ma L, et al. Ku70 Senses HTLV-1 DNA and Modulates HTLV-1 Replication. J Immunol. 2017;199:2475-2482 pubmed publisher
    ..These findings may provide new angles to understand host defenses against HTLV-1 infection and HTLV-1-associated diseases. ..

More Information

Publications17

  1. Burgman P, Ouyang H, Peterson S, Chen D, Li G. Heat inactivation of Ku autoantigen: possible role in hyperthermic radiosensitization. Cancer Res. 1997;57:2847-50 pubmed
    ..shown a constitutive DNA-binding factor in rodent cells that is inactivated by heat shock to be identical to Ku autoantigen. Ku, consisting of an Mr 70,000 (Ku70) and an Mr 86,000 (Ku80) subunit, is a heterodimeric nuclear protein and ..
  2. Qi X, Su X, Guo H, Qi J, Cheng H. A ku70 null mutant improves gene targeting frequency in the fungal pathogen Verticillium dahliae. World J Microbiol Biotechnol. 2015;31:1889-97 pubmed
    ..8 and 34.7% compared with 0.3 and 0.5 % in the wild-type strain. The ΔVdku70 strain will be a valuable tool to generate deletion strains when studying factors that underlie virulence and pathogenesis in this filamentous fungus. ..
  3. Lindström R, Lindholm P, Palgi M, Saarma M, Heino T. In vivo screening reveals interactions between Drosophila Manf and genes involved in the mitochondria and the ubiquinone synthesis pathway. BMC Genet. 2017;18:52 pubmed publisher
    ..Our data supports the functional importance of these evolutionarily significant proteins and provides new insights for the future studies. ..
  4. Hollingworth R, Horniblow R, Forrest C, Stewart G, Grand R. Localization of Double-Strand Break Repair Proteins to Viral Replication Compartments following Lytic Reactivation of Kaposi's Sarcoma-Associated Herpesvirus. J Virol. 2017;91: pubmed publisher
    ..Overall, this study extends our understanding of the virus-host interactions that occur during lytic replication of KSHV and provides a deeper insight into how the DDR is manipulated during viral infection. ..
  5. Zhang Z, Zheng F, Yu Z, Hao J, Chen M, Yu W, et al. XRCC5 cooperates with p300 to promote cyclooxygenase-2 expression and tumor growth in colon cancers. PLoS ONE. 2017;12:e0186900 pubmed publisher
  6. Zelensky A, Schimmel J, Kool H, Kanaar R, Tijsterman M. Inactivation of Pol ? and C-NHEJ eliminates off-target integration of exogenous DNA. Nat Commun. 2017;8:66 pubmed publisher
    ..Here the authors show that repression of polymerase ? and classical non-homologous recombination eliminates random integration. ..
  7. Ma J, Hui P, Meng W, Wang N, Xiang S. Ku70 inhibits gemcitabine-induced DNA damage and pancreatic cancer cell apoptosis. Biochem Biophys Res Commun. 2017;484:746-752 pubmed publisher
    ..The results of this preclinical study imply that Ku70 might be a primary resistance factor of gemcitabine, and Ku70 silence could significantly chemo-sensitize gemcitabine in pancreatic cancer cells. ..
  8. Sell C. Ku circles the telomere?. Aging (Albany NY). 2011;3:344-5 pubmed