Genomes and Genes
dna binding proteins
Summary: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
- Moujalled D, Grubman A, Acevedo K, Yang S, Ke Y, Moujalled D, et al. TDP-43 mutations causing amyotrophic lateral sclerosis are associated with altered expression of RNA-binding protein hnRNP K and affect the Nrf2 antioxidant pathway. Hum Mol Genet. 2017;26:1732-1746 pubmed publisher..Our findings indicate that further exploration of the interplay between hnRNP K (or other hnRNPs) and Nrf2-mediated antioxidant signaling is warranted and may be an important driver for motor neuron degeneration in ALS. ..
- Tempera I, De Leo A, Kossenkov A, Cesaroni M, Song H, Dawany N, et al. Identification of MEF2B, EBF1, and IL6R as Direct Gene Targets of Epstein-Barr Virus (EBV) Nuclear Antigen 1 Critical for EBV-Infected B-Lymphocyte Survival. J Virol. 2016;90:345-55 pubmed publisher..Identification of EBNA1 target genes provides potential new targets for therapeutic intervention in EBV-associated disease. ..
- Obulhasim G, Yasen M, Kajino K, Mogushi K, Tanaka S, Mizushima H, et al. Up-regulation of dbpA mRNA in hepatocellular carcinoma associated with metabolic syndrome. Hepatol Int. 2013;7:215-25 pubmed publisher..The demethylation-related epigenetic activation may be one of the regulating factors for HCC patients with MS. ..
- Genbacev O, Larocque N, Ona K, Prakobphol A, Garrido Gomez T, Kapidzic M, et al. Integrin ?4-positive human trophoblast progenitors: functional characterization and transcriptional regulation. Hum Reprod. 2016;31:1300-14 pubmed publisher..O., A.P., T.G.-G., M.K., A.B., M.G. have nothing to disclose. S.J.F. received licensing fees and royalties from SeraCare Life Sciences for trisomic TBPC lines that were derived according to the methods described in this manuscript. N/A. ..
- Plecha S, Withey J. Mechanism for inhibition of Vibrio cholerae ToxT activity by the unsaturated fatty acid components of bile. J Bacteriol. 2015;197:1716-25 pubmed publisher..This differs from the inhibitory mechanism of a synthetic ToxT inhibitor, virstatin, which inhibits ToxT dimerization. Understanding the mechanisms for inhibition of virulence could lead to better cholera therapeutics. ..
- Cheng Z, Lin M, Rikihisa Y. Ehrlichia chaffeensis proliferation begins with NtrY/NtrX and PutA/GlnA upregulation and CtrA degradation induced by proline and glutamine uptake. MBio. 2014;5:e02141 pubmed publisher..Our findings will advance the field's current conceptual paradigm on regulation of obligatory intracellular nutrition, metabolism, and growth. ..
- Silva L, Baptista J, Batley C, Andrews S, Saraiva L. The Di-iron RIC Protein (YtfE) of Escherichia coli Interacts with the DNA-Binding Protein from Starved Cells (Dps) To Diminish RIC Protein-Mediated Redox Stress. J Bacteriol. 2018;200: pubmed publisher..Altogether, we provide evidence for the formation of a new bacterial protein complex and reveal a novel contribution for Dps in bacterial redox stress protection. ..
- Brockman K, Azzari P, Branstool M, Atack J, Schulz B, Jen F, et al. Epigenetic Regulation Alters Biofilm Architecture and Composition in Multiple Clinical Isolates of Nontypeable Haemophilus influenzae. MBio. 2018;9: pubmed publisher..The findings of this work could be significant in the design of improved strategies against NTHI infections, as well as diseases due to other pathogens that utilize a phasevarion. ..
- MacNair L, Xiao S, Miletic D, Ghani M, Julien J, Keith J, et al. MTHFSD and DDX58 are novel RNA-binding proteins abnormally regulated in amyotrophic lateral sclerosis. Brain. 2016;139:86-100 pubmed publisher..This discovery-based approach has for the first time revealed translational changes in motor neurons of a TDP-43 mouse model, identifying DDX58 and MTHFSD as two TDP-43 targets that are misregulated in amyotrophic lateral sclerosis. ..
- Moujalled D, James J, Parker S, Lidgerwood G, Duncan C, Meyerowitz J, et al. Kinase Inhibitor Screening Identifies Cyclin-Dependent Kinases and Glycogen Synthase Kinase 3 as Potential Modulators of TDP-43 Cytosolic Accumulation during Cell Stress. PLoS ONE. 2013;8:e67433 pubmed publisher..This knowledge provides a valuable insight into the mechanisms controlling abnormal cytoplasmic TDP-43 accumulation and may herald new opportunities for kinase modulation-based therapeutic intervention in ALS and FTLD. ..
- Bianchi N, Cosenza L, Lampronti I, Finotti A, Breveglieri G, Zuccato C, et al. Structural and Functional Insights on an Uncharacterized AÎ³-Globin-Gene Polymorphism Present in Four Î²0-Thalassemia Families with High Fetal Hemoglobin Levels. Mol Diagn Ther. 2016;20:161-73 pubmed publisher..Additionally, we report evidence suggesting that the AÎ³-globin gene +25(Gâ†’A) polymorphism decreases the efficiency of the interaction between this sequence and specific DNA binding protein complexes. ..
- Kemp M. DNA damage-induced ATM- and Rad-3-related (ATR) kinase activation in non-replicating cells is regulated by the XPB subunit of transcription factor IIH (TFIIH). J Biol Chem. 2017;292:12424-12435 pubmed publisher..Together, these results reveal a previously unknown role for transcription factor IIH in ATR kinase activation in non-replicating, non-cycling cells. ..
- Lu F, Wikramasinghe P, Norseen J, Tsai K, Wang P, Showe L, et al. Genome-wide analysis of host-chromosome binding sites for Epstein-Barr Virus Nuclear Antigen 1 (EBNA1). Virol J. 2010;7:262 pubmed publisher..We conclude that EBNA1 can interact with a large number of cellular genes and chromosomal loci in latently infected cells, but that these sites are likely to represent a complex ensemble of direct and indirect EBNA1 binding sites. ..
- Dulmage K, Todor H, Schmid A. Growth-Phase-Specific Modulation of Cell Morphology and Gene Expression by an Archaeal Histone Protein. MBio. 2015;6:e00649-15 pubmed publisher..Given that archaea possess the ancestral form of eukaryotic histone, this study has important implications for understanding the evolution of histone function. ..
- Pomraning K, Kim Y, Nicora C, Chu R, Bredeweg E, Purvine S, et al. Multi-omics analysis reveals regulators of the response to nitrogen limitation in Yarrowia lipolytica. BMC Genomics. 2016;17:138 pubmed publisher..Regulatory proteins, including kinases and DNA binding proteins, are particularly enriched for phosphorylation...
- Kim T, Kim M, Lee J, Kim S, Je J, Lee Y, et al. CCCTC-binding factor controls the homeostatic maintenance and migration of Langerhans cells. J Allergy Clin Immunol. 2015;136:713-24 pubmed publisher..Our results show that CTCF positively regulates the homeostatic pool and the efficient emigration of LCs, which are required for modulating the functional immune network of the skin. ..
- Matsumoto A, Suzuki H, Fukatsu R, Shimizu H, Suzuki Y, Hisanaga K. An autopsy case of frontotemporal lobar degeneration with the appearance of fused in sarcoma inclusions (basophilic inclusion body disease) clinically presenting corticobasal syndrome. Neuropathology. 2016;36:77-87 pubmed publisher..Our case suggested further pathological heterogeneity in CBS than had previously been reported. It is necessary to consider FTLD-FUS (BIBD) as a background pathology for CBS in the future. ..
- Ansseau E, Eidahl J, Lancelot C, Tassin A, Mattéotti C, Yip C, et al. Homologous Transcription Factors DUX4 and DUX4c Associate with Cytoplasmic Proteins during Muscle Differentiation. PLoS ONE. 2016;11:e0146893 pubmed publisher..Further investigations are on-going to evaluate whether these interactions play roles during muscle regeneration as previously suggested for DUX4c. ..
- Leyn S, Rodionova I, Li X, Rodionov D. Novel Transcriptional Regulons for Autotrophic Cycle Genes in Crenarchaeota. J Bacteriol. 2015;197:2383-91 pubmed publisher..Novel regulons described for the first time in this work provide a basis for understanding the mechanisms of transcriptional regulation of autotrophic pathways in Archaea. ..
- Nguyen Khac F, Lesty C, Eclache V, Couronné L, Kosmider O, Andrieux J, et al. Chromosomal abnormalities in transformed Ph-negative myeloproliferative neoplasms are associated to the transformation subtype and independent of JAK2 and the TET2 mutations. Genes Chromosomes Cancer. 2010;49:919-27 pubmed publisher
- ChiÃ² A, Calvo A, Moglia C, Restagno G, Ossola I, Brunetti M, et al. Amyotrophic lateral sclerosis-frontotemporal lobar dementia in 3 families with p.Ala382Thr TARDBP mutations. Arch Neurol. 2010;67:1002-9 pubmed publisher..Three apparently unrelated families with familial ALS carrying the p.Ala382Thr TARDBP missense mutation developed FTLD. In these families, FTLD cosegregates with ALS. Patients with ALS carrying TARDBP mutations may develop FTLD. ..
- Choi H, Lee J, Kim H, Nam H, Shin H, Kim D, et al. Bcl3-dependent stabilization of CtBP1 is crucial for the inhibition of apoptosis and tumor progression in breast cancer. Biochem Biophys Res Commun. 2010;400:396-402 pubmed publisher..Our study reveals a novel combinatorial role for Bcl3 and CtBP1, providing an explanation for the acquisition of resistance to apoptosis in cancer cells, which is a major requirement for cancer development. ..
- Jang S, Kim J, Kim C, An J, Kang E, Kim C, et al. Control of transferrin expression by ?-amyloid through the CP2 transcription factor. FEBS J. 2010;277:4054-65 pubmed publisher..Taken together, these results suggest that CP2 contributes to the pathogenesis of Alzheimer's disease by inducing transferrin expression via up-regulating its transcription. ..
- Bindhya S, Balachandar V, Sudha S, Mohana Devi S, Varsha P, Kandasamy K, et al. Assessment of occupational cytogenetic risk, among petrol station workers. Bull Environ Contam Toxicol. 2010;85:121-4 pubmed publisher..In conclusion, these datas indicate that petrol pump workers under risk group should be monitored for any long-term adverse effects of the exposure. ..
- Laird K, Briggs L, Boss J, Summers M, Garvie C. Solution structure of the heterotrimeric complex between the interaction domains of RFX5 and RFXAP from the RFX gene regulatory complex. J Mol Biol. 2010;403:40-51 pubmed publisher..The clustering of hydrophobic residues from different regions of RFXAP(C) suggests a potential binding site for RFXB. ..
- Minobe K, Ono R, Matsumine A, Shibata Minoshima F, Izawa K, Oki T, et al. Expression of ADAMTS4 in Ewing's sarcoma. Int J Oncol. 2010;37:569-81 pubmed..We also demonstrated that ADAMTS4 protein was highly expressed in tumor samples of the patients with EWS by using immunohistochemistry. These results suggest that ADAMTS4 is a novel tumor marker for EWS. ..
- Marshall L, Dunham L, Major E. Transcription factor Spi-B binds unique sequences present in the tandem repeat promoter/enhancer of JC virus and supports viral activity. J Gen Virol. 2010;91:3042-52 pubmed publisher..These results suggest that Spi-B could regulate JCV gene expression in susceptible cells, and may play an important role in JCV activity in the immune and nervous systems. ..
- Chatterjee T, Chakraborti S, Joshi P, Singh S, Gupta V, Chakrabarti P. The effect of zinc oxide nanoparticles on the structure of the periplasmic domain of the Vibrio cholerae ToxR protein. FEBS J. 2010;277:4184-94 pubmed publisher..Isothermal titration calorimetry, used to quantify the thermodynamics of binding of ToxRp with ZnO NPs, showed an exothermic binding isotherm (?H = -9.8 kcal·mol(-1) and ?S = -5.17 cal·mol(-1)·K(-1)). ..
- Chen H, Gonzalez J, Brennick J, Liu M, Yan S. Immunohistochemical patterns of ProEx C in vulvar squamous lesions: detection of overexpression of MCM2 and TOP2A. Am J Surg Pathol. 2010;34:1250-7 pubmed publisher..Overall, ProEx C is a useful proliferation marker for high-grade VIN analogous to the staining patterns reported in high-grade cervical intraepithelial neoplasia. ..
- Baldwin M, O Brien P. Nonspecific DNA binding and coordination of the first two steps of base excision repair. Biochemistry. 2010;49:7879-91 pubmed publisher..AAG is not displaced into solution but remains bound to an adjacent undamaged site. We propose that nonspecific DNA binding interactions allow transient exposure of the abasic site so that it can be captured by APE1. ..
- Xu D, Muniandy P, Leo E, Yin J, Thangavel S, Shen X, et al. Rif1 provides a new DNA-binding interface for the Bloom syndrome complex to maintain normal replication. EMBO J. 2010;29:3140-55 pubmed publisher..Our data suggest that Rif1 provides a new DNA-binding interface for the BLM complex to restart stalled replication forks. ..
- Cejka P, Cannavo E, Polaczek P, Masuda Sasa T, Pokharel S, Campbell J, et al. DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2. Nature. 2010;467:112-6 pubmed publisher..We suggest that Top3-Rmi1 and MRX are important for recruitment of the Sgs1-Dna2 complex to DSBs. Our experiments provide a mechanistic framework for understanding the initial steps of recombinational DNA repair in eukaryotes. ..
- Coutandin D, Ou H, Lohr F, Dötsch V. Tracing the protectors path from the germ line to the genome. Proc Natl Acad Sci U S A. 2010;107:15318-25 pubmed publisher..This suggests that p53 is the most recent evolutionary member of this protein family and predicts a mechanism for p53 activation. ..
- Lee S, Chung H, Devaraj B, Iwaizumi M, Han H, Hwang D, et al. Microsatellite alterations at selected tetranucleotide repeats are associated with morphologies of colorectal neoplasias. Gastroenterology. 2010;139:1519-25 pubmed publisher..Loss of hMSH3 corresponds with multiple tetranucleotide frameshifts. The association between EMAST and ulcerated tumors might result from increased inflammation. ..
- Maier J, Volz Y, Berger C, Schneider S, Cato A. Depletion of the cellular levels of Bag-1 proteins attenuates phorbol ester-induced downregulation of I?B? and nuclear accumulation of NF-?B. Biochem Biophys Res Commun. 2010;401:406-11 pubmed publisher..Thus Bag-1 proteins function as regulators of the action of selective transcription factors. ..
- Lai A, Fatemi M, Dhasarathy A, Malone C, Sobol S, Geigerman C, et al. DNA methylation prevents CTCF-mediated silencing of the oncogene BCL6 in B cell lymphomas. J Exp Med. 2010;207:1939-50 pubmed publisher..These data indicate that BCL6 expression is maintained during lymphomagenesis in part through DNA methylation that prevents CTCF-mediated silencing...
- Kaur S, White T, DiGuilio A, Glavy J. The discovery of a Werner Helicase Interacting Protein (WHIP) association with the nuclear pore complex. Cell Cycle. 2010;9:3106-11 pubmed..We thus identify WHIP as a partner/component of the NE/NPC and set forth to investigate a role for the protein positioned at the NPC...
- Kanu N, Penicud K, Hristova M, Wong B, Irvine E, Plattner F, et al. The ATM cofactor ATMIN protects against oxidative stress and accumulation of DNA damage in the aging brain. J Biol Chem. 2010;285:38534-42 pubmed publisher..These results suggest that ATMIN mediates ATM activation by oxidative stress, and thereby ATMIN protects the aging brain by preventing accumulation of DNA damage. ..
- Altschul S, Wootton J, Zaslavsky E, Yu Y. The construction and use of log-odds substitution scores for multiple sequence alignment. PLoS Comput Biol. 2010;6:e1000852 pubmed publisher..We illustrate how simple BILD score based strategies can enhance the recognition of DNA binding domains, including the Api-AP2 domain in Toxoplasma gondii and Plasmodium falciparum. ..
- Zhang Z, Yuan S, Xu F, Yang H, Zhang N, Cheng J, et al. The plastid hexokinase pHXK: a node of convergence for sugar and plastid signals in Arabidopsis. FEBS Lett. 2010;584:3573-9 pubmed publisher..The data suggest that pHXK may be a node of convergence for sugar-mediated and PGE-derived signals in Arabidopsis. ..
- Geser F, Robinson J, Malunda J, Xie S, Clark C, Kwong L, et al. Pathological 43-kDa transactivation response DNA-binding protein in older adults with and without severe mental illness. Arch Neurol. 2010;67:1238-50 pubmed publisher..Finally, our data provide an age-related baseline for the development of whole-brain pathological TDP-43 evolution schemata. ..
- Zhou Q, Kalderon D. Costal 2 interactions with Cubitus interruptus (Ci) underlying Hedgehog-regulated Ci processing. Dev Biol. 2010;348:47-57 pubmed publisher..We suggest that this regulatory activity of Cos2 depends on a specific nucleotide-bound conformation that may be regulated by Hh. ..
- Chi P, Chen Y, Zhang L, Guo X, Wongvipat J, Shamu T, et al. ETV1 is a lineage survival factor that cooperates with KIT in gastrointestinal stromal tumours. Nature. 2010;467:849-53 pubmed publisher..It also represents a novel mechanism of oncogenic transcription factor activation. ..
- Klammer H, KADHIM M, Iliakis G. Evidence of an adaptive response targeting DNA nonhomologous end joining and its transmission to bystander cells. Cancer Res. 2010;70:8498-506 pubmed publisher..The results point to enhanced NHEJ as a mechanism of AR and suggest that AR may be transmitted to bystander cells through factors generating replication-mediated DSBs...
- Mani R, Yu Y, Fang S, Lu M, Fanta M, Zolner A, et al. Dual modes of interaction between XRCC4 and polynucleotide kinase/phosphatase: implications for nonhomologous end joining. J Biol Chem. 2010;285:37619-29 pubmed publisher..Our results reveal that CK2-mediated phosphorylation of XRCC4 can have different effects on PNKP activity, with implications for the roles of XRCC4 and PNKP in NHEJ. ..
- May W, Hoare K, Hoare S, Reinhard M, Lee Y, Oh S. Tnk1/Kos1: a novel tumor suppressor. Trans Am Clin Climatol Assoc. 2010;121:281-92; discussion 292-3 pubmed..We now find that a cohort of patients with diffuse large B-cell lymphoma (DLBCL) display downregulation of Tnk1/Kos1 that may account for tumorigenesis in humans...
- Oancea C, Ruster B, Henschler R, Puccetti E, Ruthardt M. The t(6;9) associated DEK/CAN fusion protein targets a population of long-term repopulating hematopoietic stem cells for leukemogenic transformation. Leukemia. 2010;24:1910-9 pubmed publisher..In contrast to the L-IC cells represented by a very rare subpopulation of LT-HSC, the L-MC seem to be represented by a larger and phenotypically heterogeneous cell population. ..
- Grudzenski S, Raths A, Conrad S, Rübe C, Lobrich M. Inducible response required for repair of low-dose radiation damage in human fibroblasts. Proc Natl Acad Sci U S A. 2010;107:14205-10 pubmed publisher..Our data suggest that the cellular response to DSBs is substantially different for low vs. high radiation doses. ..
- Singh S, Narayanan S, Biswas K, Gupta A, Ahuja N, Yadav S, et al. Intragenic DNA methylation and BORIS-mediated cancer-specific splicing contribute to the Warburg effect. Proc Natl Acad Sci U S A. 2017;114:11440-11445 pubmed publisher..Our findings highlight the role of intragenic DNA methylation and DNA binding protein BORIS in cancer-specific splicing and its role in tumorigenesis. ..
- Vilgelm A, Hong S, Washington M, Wei J, Chen H, el Rifai W, et al. Characterization of ?Np73 expression and regulation in gastric and esophageal tumors. Oncogene. 2010;29:5861-8 pubmed publisher..We found that HIC1 negatively regulates ?Np73 transcription in mucosal epithelial cells. This leads to a decrease in ?Np73 protein levels and may normally control the oncogenic potential of the ?Np73 isoform. ..
- An G, Miner C, Nixon J, Kincade P, Bryant J, Tucker P, et al. Loss of Bright/ARID3a function promotes developmental plasticity. Stem Cells. 2010;28:1560-7 pubmed publisher..Furthermore, direct knockdown of human Bright resulted in colonies capable of expressing multiple lineage markers. These data suggest that repression of this single molecule confers adult somatic cells with new developmental options. ..
- Liao H, Rao H, Zhang X, Lin Y, Jie M, Fu J, et al. [Retrospective study of clinicopathological characteristics in bronchopulmonary carcinoid]. Zhongguo Fei Ai Za Zhi. 2010;13:591-7 pubmed publisher..Ki-67, Bcl-2 high expression and advanced TNM staging are the poor recurrence and prognostic factors of BPC. The radical surgery remains the treatment of choice for resectable candidates in BPC as NSCLC. ..
- McKee A, Gavett B, Stern R, Nowinski C, Cantu R, Kowall N, et al. TDP-43 proteinopathy and motor neuron disease in chronic traumatic encephalopathy. J Neuropathol Exp Neurol. 2010;69:918-29 pubmed publisher..This is the first pathological evidence that repetitive head trauma experienced in collision sports might be associated with the development of a motor neuron disease. ..
- Hsu T, Tsai H, Huang K, Luan M, Hsieh C. Sublethal levels of cadmium down-regulate the gene expression of DNA mismatch recognition protein MutS homolog 6 (MSH6) in zebrafish (Danio rerio) embryos. Chemosphere. 2010;81:748-54 pubmed publisher..Our results revealed the inhibition of Cd on MSH6 expression at both mRNA and protein levels and this mechanism may play a role in Cd genotoxicity. ..
- Woloszynska Read A, James S, Song C, Jin B, Odunsi K, Karpf A. BORIS/CTCFL expression is insufficient for cancer-germline antigen gene expression and DNA hypomethylation in ovarian cell lines. Cancer Immun. 2010;10:6 pubmed..We conclude that BORIS is insufficient for CG antigen gene expression and DNA hypomethylation in ovarian cell lines, and that additional factors are likely required for CG antigen expression in ovarian cancer. ..
- Nonaka Y, Muto H, Aizawa T, Okabe E, Myoba S, Yokoyama T, et al. STPR, a 23-amino acid tandem repeat domain, found in the human function-unknown protein ZNF821. Biochemistry. 2010;49:8367-75 pubmed publisher..STPR sequences are found in many proteins from vertebrates, insects, and nematodes. Some of the consensus amino acid residues would be responsible for DNA binding and concomitant increases in ?-helix structure content. ..
- Choudhury A, Nelson L, Teo M, Chilka S, Bhattarai S, Johnston C, et al. MRE11 expression is predictive of cause-specific survival following radical radiotherapy for muscle-invasive bladder cancer. Cancer Res. 2010;70:7017-26 pubmed publisher..MRE11 expression may ultimately allow patient selection for radiotherapy or cystectomy, thus improving overall cure rates. ..
- Gao X, Wang Z, Martin J, Abubucker S, Zhang X, Mitreva M, et al. Identification of hookworm DAF-16/FOXO response elements and direct gene targets. PLoS ONE. 2010;5:e12289 pubmed publisher..Further investigation of these target genes will provide insights into the molecular basis by which Ac-DAF-16 regulates its downstream gene network in hookworm infection. ..
- Kodach L, Jacobs R, Heijmans J, van Noesel C, Langers A, Verspaget H, et al. The role of EZH2 and DNA methylation in the silencing of the tumour suppressor RUNX3 in colorectal cancer. Carcinogenesis. 2010;31:1567-75 pubmed publisher..These results would predict that inhibitors of EZH2 and histone methylation would enhance the effects of demethylating agents in cancer therapy. ..
- Engin A, Karahalil B, Engin A, Karakaya A. Oxidative stress, Helicobacter pylori, and OGG1 Ser326Cys, XPC Lys939Gln, and XPD Lys751Gln polymorphisms in a Turkish population with colorectal carcinoma. Genet Test Mol Biomarkers. 2010;14:559-64 pubmed publisher..094) did not associate with the increased risk of cancer progression, despite the increased oxidative stress in cancer patients. Seropositivity of H. pylori IgG has been found to increase the risk of colorectal carcinoma by 2.2-fold. ..
- Kohlmann A, Grossmann V, Klein H, Schindela S, Weiss T, Kazak B, et al. Next-generation sequencing technology reveals a characteristic pattern of molecular mutations in 72.8% of chronic myelomonocytic leukemia by detecting frequent alterations in TET2, CBL, RAS, and RUNX1. J Clin Oncol. 2010;28:3858-65 pubmed publisher..Here, NGS screening has been demonstrated to support a comprehensive characterization of the molecular background in CMML. A pattern of molecular mutations translates into different biologic and prognostic categories of CMML. ..
- Fertey J, Ammermann I, Winkler M, Stöger R, Iftner T, Stubenrauch F. Interaction of the papillomavirus E8--E2C protein with the cellular CHD6 protein contributes to transcriptional repression. J Virol. 2010;84:9505-15 pubmed publisher..This suggests that repression of the E6/E7 promoter by E2 and E8--E2C involves multiple interactions with host cell proteins through different protein domains. ..
- Schaap Nutt A, D Angelo C, Amaro Carambot E, Nolan S, Davis S, Wise S, et al. Recombinant human parainfluenza virus type 2 with mutations in V that permit cellular interferon signaling are not attenuated in non-human primates. Virology. 2010;406:65-79 pubmed publisher..This indicates that loss of HPIV2's ability to inhibit IFN signaling is insufficient to attenuate virus replication in vivo as long as IFN induction is still inhibited. ..
- Kavitha C, Choudhary B, Raghavan S, Muniyappa K. Differential regulation of MRN (Mre11-Rad50-Nbs1) complex subunits and telomerase activity in cancer cells. Biochem Biophys Res Commun. 2010;399:575-80 pubmed publisher..These studies raise the possibility of developing anti-cancer drugs targeting MRN complex subunits to sensitize a subset of cancer cells to radio- and/or chemotherapy. ..
- Hanova M, Stetina R, Vodickova L, Vaclavikova R, Hlavac P, Smerhovsky Z, et al. Modulation of DNA repair capacity and mRNA expression levels of XRCC1, hOGG1 and XPC genes in styrene-exposed workers. Toxicol Appl Pharmacol. 2010;248:194-200 pubmed publisher..However, negative correlations of SSBs and mRNA expression levels of XRCC1, hOGG1 and XPC with styrene exposure warrant further highly-targeted study. ..
- Silva P, Pereira C, Dalebout T, Spaan W, Bredenbeek P. An RNA pseudoknot is required for production of yellow fever virus subgenomic RNA by the host nuclease XRN1. J Virol. 2010;84:11395-406 pubmed publisher..These results underscore the importance of the RNA pseudoknot in stalling XRN1 and also demonstrate that it is the sole viral requirement for sfRNA production. ..
- Kozlov A, Eggington J, Cox M, Lohman T. Binding of the dimeric Deinococcus radiodurans single-stranded DNA binding protein to single-stranded DNA. Biochemistry. 2010;49:8266-75 pubmed publisher..These common features for SSB proteins having multiple DNA binding domains enable versatility of SSB function in vivo. ..
- de Bie P, Zaaroor Regev D, Ciechanover A. Regulation of the Polycomb protein RING1B ubiquitination by USP7. Biochem Biophys Res Commun. 2010;400:389-95 pubmed publisher..Although, USP7 directly and specifically deubiquitinates RING1B in vitro and in vivo, it does not discriminate between the activating and proteolysis-targeting modes of ubiquitination, and therefore has a stabilizing effect on RING1B. ..
- Kuttykrishnan S, Sabina J, Langton L, Johnston M, Brent M. A quantitative model of glucose signaling in yeast reveals an incoherent feed forward loop leading to a specific, transient pulse of transcription. Proc Natl Acad Sci U S A. 2010;107:16743-8 pubmed publisher..Finally, the model correctly predicts how lesions in the feed forward loop change the kinetics of induction of HXT4 expression. ..
- Lopez Mosqueda J, Maas N, Jonsson Z, Defazio Eli L, Wohlschlegel J, Toczyski D. Damage-induced phosphorylation of Sld3 is important to block late origin firing. Nature. 2010;467:479-83 pubmed publisher..These data indicate that the intra-S-phase checkpoint functions to block late origin firing in adverse conditions to prevent genomic instability and maximize cell survival. ..