vinculin

Summary

Summary: A cytoskeletal protein associated with cell-cell and cell-matrix interactions. The amino acid sequence of human vinculin has been determined. The protein consists of 1066 amino acid residues and its gene has been assigned to chromosome 10.

Top Publications

  1. Taguchi K, Ishiuchi T, Takeichi M. Mechanosensitive EPLIN-dependent remodeling of adherens junctions regulates epithelial reshaping. J Cell Biol. 2011;194:643-56 pubmed publisher
    ..b>Vinculin was required for general AJ formation, and it cooperated with EPLIN to maintain the ZA...
  2. Goldmann W, Auernheimer V, Thievessen I, Fabry B. Vinculin, cell mechanics and tumour cell invasion. Cell Biol Int. 2013;37:397-405 pubmed publisher
    The focal adhesion protein, vinculin, is important for transmitting mechanical forces and orchestrating mechanical signalling events...
  3. Chen S, Wang R, Li Q, Tang D. Abl knockout differentially affects p130 Crk-associated substrate, vinculin, and paxillin in blood vessels of mice. Am J Physiol Heart Circ Physiol. 2009;297:H533-9 pubmed publisher
    ..It has been known that vinculin is a structural protein that links actin filaments to extracellular matrix via transmembrane integrins, whereas ..
  4. Park H, Lee J, Gouin E, Cossart P, Izard T. The rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin. J Biol Chem. 2011;286:35096-103 pubmed publisher
    ..Here we report that the cell surface antigen sca4 of Rickettsia co-localizes with vinculin in cells at sites of focal adhesions in sca4-transfected cells and that sca4 binds to and activates vinculin ..
  5. Möhl C, Kirchgessner N, Schafer C, Küpper K, Born S, Diez G, et al. Becoming stable and strong: the interplay between vinculin exchange dynamics and adhesion strength during adhesion site maturation. Cell Motil Cytoskeleton. 2009;66:350-64 pubmed publisher
    ..Here, we tested the exchange dynamics of GFP-vinculin as marker protein of focal adhesions using the method of Fluorescence Recovery After Photobleaching...
  6. Del Rio A, Perez Jimenez R, Liu R, Roca Cusachs P, Fernandez J, Sheetz M. Stretching single talin rod molecules activates vinculin binding. Science. 2009;323:638-41 pubmed publisher
    ..force on the interaction between talin, a protein that links liganded membrane integrins to the cytoskeleton, and vinculin, a focal adhesion protein that is activated by talin binding, leading to reorganization of the cytoskeleton...
  7. Umemoto T, Tanaka K, Ueda K, Kioka N. Tyrosine phosphorylation of vinexin in v-Src-transformed cells attenuates the affinity for vinculin. Biochem Biophys Res Commun. 2009;387:191-5 pubmed publisher
    Vinexin is an adaptor-type focal adhesion protein that interacts with vinculin. Here, we report the tyrosine phosphorylation of vinexin alpha in v-Src-transformed NIH3T3 cells...
  8. Nelson E, Folkmann A, Henry M, DeMali K. Vinculin activators target integrins from within the cell to increase melanoma sensitivity to chemotherapy. Mol Cancer Res. 2011;9:712-23 pubmed publisher
    ..An attractive alternative approach is to target proteins, such as vinculin, that associate with the integrin cytoplasmic domains and regulate its ligand-binding properties...
  9. Golji J, Wendorff T, Mofrad M. Phosphorylation primes vinculin for activation. Biophys J. 2012;102:2022-30 pubmed publisher
    b>Vinculin phosphorylation has been implicated as a potential mechanism for focal adhesion growth and maturation. Four vinculin residues-Y100, S1033, S1045, and Y1065-are phosphorylated by kinases during focal adhesion maturation...

More Information

Publications62

  1. Xiao H, Eves R, Yeh C, Kan W, Xu F, Mak A, et al. Phorbol ester-induced podosomes in normal human bronchial epithelial cells. J Cell Physiol. 2009;218:366-75 pubmed publisher
    ..Podosomes and circular dorsal ruffles may be important for epithelial cell migration and invasion, thus contributing to respiratory epithelial repair and regeneration. ..
  2. Wolfenson H, Bershadsky A, Henis Y, Geiger B. Actomyosin-generated tension controls the molecular kinetics of focal adhesions. J Cell Sci. 2011;124:1425-32 pubmed publisher
    ..We measured the early changes in k(off) values for three FA proteins (vinculin, paxillin and zyxin) upon inhibition of actomyosin-generated forces using two methods - high temporal resolution ..
  3. Peng X, Maiers J, Choudhury D, Craig S, DeMali K. ?-Catenin uses a novel mechanism to activate vinculin. J Biol Chem. 2012;287:7728-37 pubmed publisher
    b>Vinculin, an actin-binding protein, is emerging as an important regulator of adherens junctions. In focal-adhesions, vinculin is activated by simultaneous binding of talin to its head domain and actin filaments to its tail domain...
  4. Hu J, Teng J, Ding N, He M, Sun Y, Yu A, et al. FAAP, a novel murine protein, is involved in cell adhesion through regulating vinculin-paxillin association. Front Biosci. 2008;13:7123-31 pubmed
    ..protein (FAAP), encoded by murine D10Wsu52e gene, is highly homologous to human HSPC117, which interacts with vinculin and talin...
  5. Critchley D, Gingras A. Talin at a glance. J Cell Sci. 2008;121:1345-7 pubmed publisher
  6. van den Dries K, Meddens M, de Keijzer S, Shekhar S, Subramaniam V, Figdor C, et al. Interplay between myosin IIA-mediated contractility and actin network integrity orchestrates podosome composition and oscillations. Nat Commun. 2013;4:1412 pubmed publisher
    ..The tension transmitted to the ring recruits vinculin and zyxin and preserves overall podosome integrity...
  7. Sun N, Critchley D, Paulin D, Li Z, Robson R. Human alpha-synemin interacts directly with vinculin and metavinculin. Biochem J. 2008;409:657-67 pubmed
    ..We have previously identified interactions of avian synemin with alpha-actinin and vinculin. Avian synemin, however, is expressed as only one form, whereas human synemin is expressed as two major splice ..
  8. Fraley S, Feng Y, Krishnamurthy R, Kim D, Celedon A, Longmore G, et al. A distinctive role for focal adhesion proteins in three-dimensional cell motility. Nat Cell Biol. 2010;12:598-604 pubmed publisher
    ..Live-cell microscopy shows that for cells fully embedded in a 3D matrix, focal adhesion proteins, including vinculin, paxillin, talin, alpha-actinin, zyxin, VASP, FAK and p130Cas, do not form aggregates but are diffusely ..
  9. Huveneers S, de Rooij J. Mechanosensitive systems at the cadherin-F-actin interface. J Cell Sci. 2013;126:403-13 pubmed publisher
    ..a key mechanosensitive system at cell-cell junctions; the cadherin-F-actin interface, at which ?-catenin and vinculin form a central module...
  10. Ishiyama N, Tanaka N, Abe K, Yang Y, Abbas Y, Umitsu M, et al. An autoinhibited structure of ?-catenin and its implications for vinculin recruitment to adherens junctions. J Biol Chem. 2013;288:15913-25 pubmed publisher
    Catenin is an actin- and vinculin-binding protein that regulates cell-cell adhesion by interacting with cadherin adhesion receptors through ?-catenin, but the mechanisms by which it anchors the cadherin-catenin complex to the actin ..
  11. Shen K, Tolbert C, Guilluy C, Swaminathan V, Berginski M, Burridge K, et al. The vinculin C-terminal hairpin mediates F-actin bundle formation, focal adhesion, and cell mechanical properties. J Biol Chem. 2011;286:45103-15 pubmed publisher
    b>Vinculin is an essential and highly conserved cell adhesion protein, found at both focal adhesions and adherens junctions, where it couples integrins or cadherins to the actin cytoskeleton...
  12. Le Clainche C, Dwivedi S, Didry D, Carlier M. Vinculin is a dually regulated actin filament barbed end-capping and side-binding protein. J Biol Chem. 2010;285:23420-32 pubmed publisher
    The focal adhesion protein vinculin is an actin-binding protein involved in the mechanical coupling between the actin cytoskeleton and the extracellular matrix...
  13. Wirth V, List F, Diez G, Goldmann W. Vinculin's C-terminal region facilitates phospholipid membrane insertion. Biochem Biophys Res Commun. 2010;398:433-7 pubmed publisher
    The focal adhesion protein vinculin has been implicated in associating with soluble and membranous phospholipids. Here, we investigated the intermolecular interactions of two vinculin tail domains with membrane phospholipids...
  14. Thievessen I, Thompson P, Berlemont S, Plevock K, Plotnikov S, Zemljic Harpf A, et al. Vinculin-actin interaction couples actin retrograde flow to focal adhesions, but is dispensable for focal adhesion growth. J Cell Biol. 2013;202:163-77 pubmed publisher
    ..How dynamic F-actin is coupled to FA is not known. We analyzed the role of vinculin in integrating F-actin and FA dynamics by vinculin gene disruption in primary fibroblasts...
  15. Thomas W, Boscher C, Chu Y, Cuvelier D, Martinez Rico C, Seddiki R, et al. ?-Catenin and vinculin cooperate to promote high E-cadherin-based adhesion strength. J Biol Chem. 2013;288:4957-69 pubmed publisher
    ..The cadherin cytoplasmic domain recruits several partners, including catenins and vinculin, at sites of cell-cell adhesion...
  16. Carisey A, Tsang R, Greiner A, Nijenhuis N, Heath N, Nazgiewicz A, et al. Vinculin regulates the recruitment and release of core focal adhesion proteins in a force-dependent manner. Curr Biol. 2013;23:271-81 pubmed publisher
    ..The molecular basis of how these sensing units are regulated, how they are implicated in transducing mechanical stimuli, and how this leads to a spatiotemporal coordination of FAs is unclear...
  17. Dumbauld D, Michael K, Hanks S, Garcia A. Focal adhesion kinase-dependent regulation of adhesive forces involves vinculin recruitment to focal adhesions. Biol Cell. 2010;102:203-213 pubmed publisher
    ..FAK expression reduced steady-state strength by 30% compared with FAK-null cells. FAK expression reduced VCL (vinculin) localization to focal adhesions by 35% independently of changes in integrin binding and localization of talin ..
  18. Gingras A, Bate N, Goult B, Patel B, Kopp P, Emsley J, et al. Central region of talin has a unique fold that binds vinculin and actin. J Biol Chem. 2010;285:29577-87 pubmed publisher
    ..8) is higher than other rod domains. Both helical bundles contain vinculin-binding sites but that in the isolated 5-helix bundle is cryptic, whereas that in the isolated 4-helix bundle is ..
  19. Magro A, Magro A, Cunningham C, Miller M. Down-regulation of vinculin upon MK886-induced apoptosis in LN18 glioblastoma cells. Neoplasma. 2007;54:517-26 pubmed
    ..addition, MK886-induced apoptosis in LN18 cells was accompanied by a decrease in the protein and mRNA levels of vinculin, but not other focal adhesion proteins...
  20. Smith S, McCann R. A C-terminal dimerization motif is required for focal adhesion targeting of Talin1 and the interaction of the Talin1 I/LWEQ module with F-actin. Biochemistry. 2007;46:10886-98 pubmed
  21. Rangarajan E, Lee J, Yogesha S, Izard T. A helix replacement mechanism directs metavinculin functions. PLoS ONE. 2010;5:e10679 pubmed publisher
    Cells require distinct adhesion complexes to form contacts with their neighbors or the extracellular matrix, and vinculin links these complexes to the actin cytoskeleton...
  22. Moese S, Selbach M, Brinkmann V, Karlas A, Haimovich B, Backert S, et al. The Helicobacter pylori CagA protein disrupts matrix adhesion of gastric epithelial cells by dephosphorylation of vinculin. Cell Microbiol. 2007;9:1148-61 pubmed
    ..Owing to the inactivation of c-Src via CagA interaction, vinculin is dephosphorylated at tyrosine residues, 100 and 1065, by corresponding phosphatases...
  23. Peng X, Nelson E, Maiers J, DeMali K. New insights into vinculin function and regulation. Int Rev Cell Mol Biol. 2011;287:191-231 pubmed publisher
    b>Vinculin is a cytoplasmic actin-binding protein enriched in focal adhesions and adherens junctions that is essential for embryonic development. Much is now known regarding the role of vinculin in governing cell-matrix adhesion...
  24. Nin V, Hernández J, Chifflet S. Hyperpolarization of the plasma membrane potential provokes reorganization of the actin cytoskeleton and increases the stability of adherens junctions in bovine corneal endothelial cells in culture. Cell Motil Cytoskeleton. 2009;66:1087-99 pubmed publisher
    ..Together with our previous work, the results of this study contribute to the idea that modifications in the PMP of nonexcitable cells participate in cellular adaptive responses involving reorganization of cytoskeletal components. ..
  25. Nhieu G, Izard T. Vinculin binding in its closed conformation by a helix addition mechanism. EMBO J. 2007;26:4588-96 pubmed
    b>Vinculin links integrin receptors to the actin cytoskeleton by binding to talin...
  26. Zhou X, Rowe R, Hiraoka N, George J, Wirtz D, Mosher D, et al. Fibronectin fibrillogenesis regulates three-dimensional neovessel formation. Genes Dev. 2008;22:1231-43 pubmed publisher
    ..These results advance a new model wherein Fn polymerization serves as a structural scaffolding that displays adhesive ligands on a mechanically ideal substratum for promoting neovessel development. ..
  27. Hirata H, Tatsumi H, Lim C, Sokabe M. Force-dependent vinculin binding to talin in live cells: a crucial step in anchoring the actin cytoskeleton to focal adhesions. Am J Physiol Cell Physiol. 2014;306:C607-20 pubmed publisher
    ..is anchored to the extracellular matrix through integrin and a variety of linker proteins including talin and vinculin. The localization of vinculin at FAs depends on mechanical forces...
  28. Halstead J, Savaskan N, van den Bout I, van Horck F, Hajdo Milasinovic A, Snell M, et al. Rac controls PIP5K localisation and PtdIns(4,5)P? synthesis, which modulates vinculin localisation and neurite dynamics. J Cell Sci. 2010;123:3535-46 pubmed publisher
    ..To clarify how increased levels of PtdIns(4,5)P? induce neurite retraction, we show that mutants of vinculin that are unable to interact with PtdIns(4,5)P?, attenuate PIP5K- and LPA-induced neurite retraction...
  29. Choi H, Pokutta S, Cadwell G, Bobkov A, Bankston L, Liddington R, et al. ?E-catenin is an autoinhibited molecule that coactivates vinculin. Proc Natl Acad Sci U S A. 2012;109:8576-81 pubmed publisher
    ..E-catenin also binds to the F-actin-binding protein vinculin, which also appears to be important in junction assembly...
  30. Koshimizu T, Kawai M, Kondou H, Tachikawa K, Sakai N, Ozono K, et al. Vinculin functions as regulator of chondrogenesis. J Biol Chem. 2012;287:15760-75 pubmed publisher
    ..mutagenesis to a murine mesenchymal chondrogenic cell line ATDC5 and isolated a clone in which the gene encoding vinculin was trapped...
  31. Tangney J, Chuang J, Janssen M, Krishnamurthy A, Liao P, Hoshijima M, et al. Novel role for vinculin in ventricular myocyte mechanics and dysfunction. Biophys J. 2013;104:1623-33 pubmed publisher
    b>Vinculin (Vcl) plays a key structural role in ventricular myocytes that, when disrupted, can lead to contractile dysfunction and dilated cardiomyopathy...
  32. Wen K, Rubenstein P, DeMali K. Vinculin nucleates actin polymerization and modifies actin filament structure. J Biol Chem. 2009;284:30463-73 pubmed publisher
    b>Vinculin links integrins to the actin cytoskeleton by binding F-actin. Little is known with respect to how this interaction occurs or affects actin dynamics...
  33. Park H, Valencia Gallardo C, Sharff A, Tran van Nhieu G, Izard T. Novel vinculin binding site of the IpaA invasin of Shigella. J Biol Chem. 2011;286:23214-21 pubmed publisher
    ..IpaA binds directly to and activates the cytoskeletal protein vinculin after injection in the host cell cytosol, and this was previously thought to be directed by two amphipathic ?-..
  34. Madl T, Sattler M. Adhesion dance with raver. Structure. 2009;17:781-3 pubmed publisher
    ..Crystal structures of raver1-vinculin reported by Izard and coworkers now suggest a possible mechanism for mRNA localization during the assembly of ..
  35. Gingras A, Ziegler W, Bobkov A, Joyce M, Fasci D, Himmel M, et al. Structural determinants of integrin binding to the talin rod. J Biol Chem. 2009;284:8866-76 pubmed publisher
    ..Thermal unfolding experiments show that integrin binding induces conformational changes in the IBS2 module, which we speculate are linked to vinculin and membrane binding.
  36. Humphries J, Wang P, Streuli C, Geiger B, Humphries M, Ballestrem C. Vinculin controls focal adhesion formation by direct interactions with talin and actin. J Cell Biol. 2007;179:1043-57 pubmed
    Focal adhesions (FAs) regulate cell migration. Vinculin, with its many potential binding partners, can interconnect signals in FAs...
  37. Ji L, Lim J, Danuser G. Fluctuations of intracellular forces during cell protrusion. Nat Cell Biol. 2008;10:1393-400 pubmed publisher
    ..cytoskelal dynamics, force fluctuations were correlated with fluctuations in F-actin turnover, flow and F-actin-vinculin coupling...
  38. Huveneers S, Oldenburg J, Spanjaard E, van der Krogt G, Grigoriev I, Akhmanova A, et al. Vinculin associates with endothelial VE-cadherin junctions to control force-dependent remodeling. J Cell Biol. 2012;196:641-52 pubmed publisher
    ..FAJs are attached to radial F-actin bundles and marked by the mechanosensory protein Vinculin. We show that endothelial hormones vascular endothelial growth factor, tumor necrosis factor ?, and most ..
  39. Ziegler W, Gingras A, Critchley D, Emsley J. Integrin connections to the cytoskeleton through talin and vinculin. Biochem Soc Trans. 2008;36:235-9 pubmed publisher
    ..integrin-binding site, a highly conserved C-terminal actin-binding site and also several binding sites for vinculin. We have determined previously the crystal structures of two domains from the talin rod, spanning residues 482-..
  40. Nolz J, Medeiros R, Mitchell J, Zhu P, Freedman B, Shimizu Y, et al. WAVE2 regulates high-affinity integrin binding by recruiting vinculin and talin to the immunological synapse. Mol Cell Biol. 2007;27:5986-6000 pubmed
    ..results in WAVE2-ARP2/3-dependent F-actin nucleation and the formation of a complex containing WAVE2, ARP2/3, vinculin, and talin...
  41. Hu K, Ji L, Applegate K, Danuser G, Waterman Storer C. Differential transmission of actin motion within focal adhesions. Science. 2007;315:111-5 pubmed
    ..Interactions between vinculin, talin, and actin filaments appear to constitute a slippage interface between the cytoskeleton and integrins, ..
  42. Bays J, Peng X, Tolbert C, Guilluy C, Angell A, Pan Y, et al. Vinculin phosphorylation differentially regulates mechanotransduction at cell-cell and cell-matrix adhesions. J Cell Biol. 2014;205:251-63 pubmed publisher
    Cells experience mechanical forces throughout their lifetimes. Vinculin is critical for transmitting these forces, yet how it achieves its distinct functions at cell-cell and cell-matrix adhesions remains unanswered...
  43. Peng X, Cuff L, Lawton C, DeMali K. Vinculin regulates cell-surface E-cadherin expression by binding to beta-catenin. J Cell Sci. 2010;123:567-77 pubmed publisher
    b>Vinculin was identified as a component of adherens junctions 30 years ago, yet its function there remains elusive. Deletion studies are consistent with the idea that vinculin is important for the organization of cell-cell junctions...
  44. Schmidt E, Gu S, Anagnostopoulou V, Alevizopoulos K, Foller M, Lang F, et al. Serum- and glucocorticoid-dependent kinase-1-induced cell migration is dependent on vinculin and regulated by the membrane androgen receptor. FEBS J. 2012;279:1231-42 pubmed publisher
    ..The cell-adhesion protein vinculin was effectively dephosphorylated in SGK1-SD-transfected cells...
  45. Mierke C. The role of vinculin in the regulation of the mechanical properties of cells. Cell Biochem Biophys. 2009;53:115-26 pubmed publisher
    b>Vinculin couples as a focal adhesion protein the extracellular matrix (ECM) through integrins to the actomyosin cytoskeleton...
  46. Palmer S, Playford M, Craig S, Schaller M, Campbell S. Lipid binding to the tail domain of vinculin: specificity and the role of the N and C termini. J Biol Chem. 2009;284:7223-31 pubmed publisher
    b>Vinculin is a highly conserved and abundant cytoskeletal protein involved in linking the actin cytoskeleton to the cell membrane at sites of cellular adhesion...
  47. Diez G, Kollmannsberger P, Mierke C, Koch T, Vali H, Fabry B, et al. Anchorage of vinculin to lipid membranes influences cell mechanical properties. Biophys J. 2009;97:3105-12 pubmed publisher
    The focal adhesion protein vinculin (1066 residues) can be separated into a 95-kDa head and a 30-kDa tail domain...
  48. Diez G, Auernheimer V, Fabry B, Goldmann W. Head/tail interaction of vinculin influences cell mechanical behavior. Biochem Biophys Res Commun. 2011;406:85-8 pubmed publisher
    This study evaluates the influence of vinculin in closed conformation on the mechanical properties of cells...
  49. Kim Kaneyama J, Takeda N, Sasai A, Miyazaki A, Sata M, Hirabayashi T, et al. Hic-5 deficiency enhances mechanosensitive apoptosis and modulates vascular remodeling. J Mol Cell Cardiol. 2011;50:77-86 pubmed publisher
    ..Mechanical stress also induced the alteration of intracellular distribution of vinculin from focal adhesions to the whole cytoplasm in SMCs...
  50. Patla I, Volberg T, Elad N, Hirschfeld Warneken V, Grashoff C, Fassler R, et al. Dissecting the molecular architecture of integrin adhesion sites by cryo-electron tomography. Nat Cell Biol. 2010;12:909-15 pubmed publisher
    ..Our findings clarify the internal architecture of focal adhesions at molecular resolution, and provide insights into their scaffolding and mechanosensory functions...
  51. Grashoff C, Hoffman B, Brenner M, Zhou R, Parsons M, Yang M, et al. Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics. Nature. 2010;466:263-6 pubmed publisher
    ..The method is applied to vinculin, a protein that connects integrins to actin filaments and whose recruitment to focal adhesions (FAs) is force-..
  52. Deakin N, Ballestrem C, Turner C. Paxillin and Hic-5 interaction with vinculin is differentially regulated by Rac1 and RhoA. PLoS ONE. 2012;7:e37990 pubmed publisher
    ..of FRET, FRAP and biochemical analyses we show that the interactions between the focal adhesion proteins vinculin and paxillin, as well as the closely related family member Hic-5 are spatially and reciprocally regulated by the ..
  53. Hsieh Y, Hsieh S, Chang Y, Hsueh C, Hsu S. The lipoxygenase inhibitor, baicalein, modulates cell adhesion and migration by up-regulation of integrins and vinculin in rat heart endothelial cells. Br J Pharmacol. 2007;151:1235-45 pubmed
    ..blot analysis indicated that baicalein increased expression levels of integrin-alpha5beta1, -alphavbeta3 and vinculin proteins...