tropomyosin

Summary

Summary: A protein found in the thin filaments of muscle fibers. It inhibits contraction of the muscle unless its position is modified by TROPONIN.

Top Publications

  1. Gaffin R, Pena J, Alves M, Dias F, Chowdhury S, Heinrich L, et al. Long-term rescue of a familial hypertrophic cardiomyopathy caused by a mutation in the thin filament protein, tropomyosin, via modulation of a calcium cycling protein. J Mol Cell Cardiol. 2011;51:812-20 pubmed publisher
    ..remodeling in a familial hypertrophic cardiomyopathy (FHC) caused by a mutation in the thin filament protein, tropomyosin (i.e., ?-TmE180G or Tm180)...
  2. Cranz Mileva S, Pamula M, Barua B, Desai B, Hong Y, Russell J, et al. A molecular evolution approach to study the roles of tropomyosin in fission yeast. PLoS ONE. 2013;8:e76726 pubmed publisher
    b>Tropomyosin, a coiled-coil protein that binds along the length of the actin filament, is a universal regulator of the actin cytoskeleton...
  3. Wei J, Gu Y, Yang J, Yang Y, Wang S, Cui S, et al. Identification and characterization of protective epitope of Trichinella spiralis paramyosin. Vaccine. 2011;29:3162-8 pubmed publisher
    ..spiralis larval challenge. The identification of a protective epitope within Ts-Pmy highlights the possibility of developing a subunit vaccine against T. spiralis infection. ..
  4. Irimia M, Maeso I, Gunning P, Garcia Fernandez J, Roy S. Internal and external paralogy in the evolution of tropomyosin genes in metazoans. Mol Biol Evol. 2010;27:1504-17 pubmed publisher
    ..We report a 22-species comparative analysis of tropomyosin (PPM) genes, which exist in a variety of forms and are implicated in the emergence of a wealth of cellular ..
  5. Scala E, Alessandri C, Palazzo P, Pomponi D, Liso M, Bernardi M, et al. IgE recognition patterns of profilin, PR-10, and tropomyosin panallergens tested in 3,113 allergic patients by allergen microarray-based technology. PLoS ONE. 2011;6:e24912 pubmed publisher
    ..Allergen microarray IgE testing increases our knowledge of the IgE immune response and related epidemiological features within and between homologous molecules better describing the patients' immunological phenotypes. ..
  6. Perz Edwards R, Irving T, Baumann B, Gore D, Hutchinson D, Krzic U, et al. X-ray diffraction evidence for myosin-troponin connections and tropomyosin movement during stretch activation of insect flight muscle. Proc Natl Acad Sci U S A. 2011;108:120-5 pubmed publisher
    ..Observed changes in X-ray reflections arising from myosin heads, actin filaments, troponin, and tropomyosin were consistent with the hypothesis that troponin bridges are the key agent of mechanical signal transduction...
  7. Ma L, Bradu A, Podoleanu A, Bloor J. Arrhythmia caused by a Drosophila tropomyosin mutation is revealed using a novel optical coherence tomography instrument. PLoS ONE. 2010;5:e14348 pubmed publisher
    ..We applied this OCT instrument to a model of Tropomyosin-associated DCM established in adult Drosophila...
  8. Mijailovich S, Li X, Del Alamo J, Griffiths R, Kecman V, Geeves M. Resolution and uniqueness of estimated parameters of a model of thin filament regulation in solution. Comput Biol Chem. 2010;34:19-33 pubmed publisher
    ..is defined by a set of nonlinear ordinary differential equations (ODEs) that predict the evolution of troponin-tropomyosin-actin and actin-myosin states...
  9. East D, Sousa D, Martin S, Edwards T, Lehman W, Mulvihill D. Altering the stability of the Cdc8 overlap region modulates the ability of this tropomyosin to bind co-operatively to actin and regulate myosin. Biochem J. 2011;438:265-73 pubmed publisher
    Tm (tropomyosin) is an evolutionarily conserved ?-helical coiled-coil protein, dimers of which form end-to-end polymers capable of associating with and stabilizing actin filaments, and regulating myosin function...

More Information

Publications95

  1. Choi H, Yim S, Xu H, Jung S, Shin S, Hu H, et al. Tropomyosin3 overexpression and a potential link to epithelial-mesenchymal transition in human hepatocellular carcinoma. BMC Cancer. 2010;10:122 pubmed publisher
    ..To our knowledge, this is the first evidence that TPM3 gets involved in migration and invasion of HCCs by modifying EMT pathway. ..
  2. Lakdawala N, Dellefave L, Redwood C, Sparks E, Cirino A, Depalma S, et al. Familial dilated cardiomyopathy caused by an alpha-tropomyosin mutation: the distinctive natural history of sarcomeric dilated cardiomyopathy. J Am Coll Cardiol. 2010;55:320-9 pubmed publisher
    ..A novel D230N missense mutation in the gene encoding alpha-tropomyosin (TPM1) was identified...
  3. Stark B, Sladewski T, Pollard L, Lord M. Tropomyosin and myosin-II cellular levels promote actomyosin ring assembly in fission yeast. Mol Biol Cell. 2010;21:989-1000 pubmed publisher
    Myosin-II (Myo2p) and tropomyosin are essential for contractile ring formation and cytokinesis in fission yeast. Here we used a combination of in vivo and in vitro approaches to understand how these proteins function at contractile rings...
  4. Kalyva A, Schmidtmann A, Geeves M. In vitro formation and characterization of the skeletal muscle α·β tropomyosin heterodimers. Biochemistry. 2012;51:6388-99 pubmed
    b>Tropomyosin (Tm) is a dimer made of two alpha helical chains associated into a parallel coiled-coil. In mammalian skeletal and cardiac muscle, the Tm is expressed from two separate genes to give the α- and β-Tm isoforms...
  5. Lam C, Yip C, Poon T, Cheng C, Ng E, Wong N, et al. Identification and characterization of tropomyosin 3 associated with granulin-epithelin precursor in human hepatocellular carcinoma. PLoS ONE. 2012;7:e40324 pubmed publisher
    ..were analyzed by SDS-PAGE, followed by mass spectrometry and the protein identity was demonstrated to be tropomyosin 3 (TPM3)...
  6. Sookrung N, Indrawattana N, Tungtrongchitr A, Bunnag C, Tantilipikorn P, Kwangsri S, et al. Allergenicity of native/recombinant tropomyosin, per a 7, of American cockroach (CR), Periplaneta americana, among CR allergic Thais. Asian Pac J Allergy Immunol. 2009;27:9-17 pubmed
    In this study, native tropomyosin (Per a 7) of American cockroach (CR), Periplaneta americana, caught in Thailand was purified...
  7. Wang C, Coluccio L. New insights into the regulation of the actin cytoskeleton by tropomyosin. Int Rev Cell Mol Biol. 2010;281:91-128 pubmed publisher
    The actin cytoskeleton is regulated by a variety of actin-binding proteins including those constituting the tropomyosin family. Tropomyosins are coiled-coil dimers that bind along the length of actin filaments...
  8. Sereda M, Hartmann S, Büttner D, Volkmer R, Hovestädt M, Brattig N, et al. Characterization of the allergen filarial tropomyosin with an invertebrate specific monoclonal antibody. Acta Trop. 2010;116:61-7 pubmed publisher
    ..Studies with a monoclonal antibody, NR1, raised against tropomyosin of the rodent filarial nematode Acanthocheilonema viteae revealed a B cell epitope common to helminths and ..
  9. Hayley M, Chevaldina T, Heeley D. Cold adaptation of tropomyosin. Biochemistry. 2011;50:6559-66 pubmed publisher
    ..Heat-induced unfolding of shark tropomyosin (2 mg/mL, 0...
  10. Kirwan J, Hodges R. Critical interactions in the stability control region of tropomyosin. J Struct Biol. 2010;170:294-306 pubmed publisher
    ..has recently described a stability control region in the two-stranded alpha-helical coiled-coil alpha-tropomyosin that accounts for overall protein stability but is not required for folding (Hodges et al., 2009)...
  11. Borovikov Y, Avrova S, Karpicheva O, Robinson P, Redwood C. The effect of the dilated cardiomyopathy-causing Glu40Lys TPM1 mutation on actin-myosin interactions during the ATPase cycle. Biochem Biophys Res Commun. 2011;411:496-500 pubmed publisher
    ..DCM can result from mutations in the gene encoding cardiac ?-tropomyosin (TM)...
  12. Jagatheesan G, Rajan S, Ahmed R, Petrashevskaya N, Boivin G, Arteaga G, et al. Striated muscle tropomyosin isoforms differentially regulate cardiac performance and myofilament calcium sensitivity. J Muscle Res Cell Motil. 2010;31:227-39 pubmed publisher
    b>Tropomyosin (TM) plays a central role in calcium mediated striated muscle contraction. There are three muscle TM isoforms: alpha-TM, beta-TM, and gamma-TM. alpha-TM is the predominant cardiac and skeletal muscle isoform...
  13. Kubo E, Hasanova N, Tanaka Y, Fatma N, Takamura Y, Singh D, et al. Protein expression profiling of lens epithelial cells from Prdx6-depleted mice and their vulnerability to UV radiation exposure. Am J Physiol Cell Physiol. 2010;298:C342-54 pubmed publisher
    ..Among the cytoskeleton-associated proteins that were highly expressed in Prdx6-deficient LECs was tropomyosin (Tm)2beta...
  14. Skau C, Kovar D. Fimbrin and tropomyosin competition regulates endocytosis and cytokinesis kinetics in fission yeast. Curr Biol. 2010;20:1415-22 pubmed publisher
    b>Tropomyosin is an important actin filament-stabilizing protein that controls the access of other essential proteins to filaments, including myosin motors, Arp2/3 complex, formin, and cofilin...
  15. Miki M, Makimura S, Sugahara Y, Yamada R, Bunya M, Saitoh T, et al. A three-dimensional FRET analysis to construct an atomic model of the actin-tropomyosin-troponin core domain complex on a muscle thin filament. J Mol Biol. 2012;420:40-55 pubmed publisher
    ..Fluorescence resonance energy transfer (FRET) was used to construct an atomic model of the actin-tropomyosin (Tm)-troponin (Tn) core domain complex...
  16. Acevedo N, Sanchez J, Erler A, Mercado D, Briza P, Kennedy M, et al. IgE cross-reactivity between Ascaris and domestic mite allergens: the role of tropomyosin and the nematode polyprotein ABA-1. Allergy. 2009;64:1635-43 pubmed publisher
    ..The objective of this study was to investigate immunoglobulin E (IgE) cross-reactivity between Ascaris and two domestic mites in the tropics...
  17. Yang J, Gu Y, Yang Y, Wei J, Wang S, Cui S, et al. Trichinella spiralis: immune response and protective immunity elicited by recombinant paramyosin formulated with different adjuvants. Exp Parasitol. 2010;124:403-8 pubmed publisher
    ..spiralis larval challenge. This indicates that the recombinant Ts-Pmy formulated with Montanide ISA206 or ISA720 may be an effective and safety vaccine strategy for trichinellosis. ..
  18. Borovikov Y, Karpicheva O, Avrova S, Robinson P, Redwood C. The effect of the dilated cardiomyopathy-causing mutation Glu54Lys of alpha-tropomyosin on actin-myosin interactions during the ATPase cycle. Arch Biochem Biophys. 2009;489:20-4 pubmed publisher
    In order to understand how the Glu54Lys mutation of alpha-tropomyosin affects actomyosin interactions, we labeled SH1 helix of myosin subfragment-1 (S1) and the actin subdomain-1 with fluorescent probes...
  19. Lassing I, Hillberg L, Höglund A, Karlsson R, SCHUTT C, Lindberg U. Tropomyosin is a tetramer under physiological salt conditions. Cytoskeleton (Hoboken). 2010;67:599-607 pubmed publisher
    b>Tropomyosin (TM) is a coiled-coil dimer of alpha-helical peptides, which self associates in a head- to-tail fashion along actin polymers, conferring stability to the microfilaments and serving a regulatory function in acto-myosin driven ..
  20. Coulton A, East D, Galinska Rakoczy A, Lehman W, Mulvihill D. The recruitment of acetylated and unacetylated tropomyosin to distinct actin polymers permits the discrete regulation of specific myosins in fission yeast. J Cell Sci. 2010;123:3235-43 pubmed publisher
    b>Tropomyosin (Tm) is a conserved dimeric coiled-coil protein, which forms polymers that curl around actin filaments in order to regulate actomyosin function...
  21. Faulkner C, Blackman L, Collings D, Cordwell S, Overall R. Anti-tropomyosin antibodies co-localise with actin microfilaments and label plasmodesmata. Eur J Cell Biol. 2009;88:357-69 pubmed publisher
    ..was sequenced and found to contain three peptides with similarity to the animal actin-binding protein tropomyosin. Western blot analysis with anti-tropomyosin antibodies confirmed the identity of this 75-kDa protein as a ..
  22. Santiago H, Bennuru S, Boyd A, Eberhard M, Nutman T. Structural and immunologic cross-reactivity among filarial and mite tropomyosin: implications for the hygiene hypothesis. J Allergy Clin Immunol. 2011;127:479-86 pubmed publisher
    ..assess the structural and immunologic relationships between the known Dermatophagoides pteronyssinus (Der p 10) tropomyosin allergen and filarial tropomyosin of Onchocerca volvulus (OvTrop)...
  23. Galinska A, Hatch V, Craig R, Murphy A, Van Eyk J, Wang C, et al. The C terminus of cardiac troponin I stabilizes the Ca2+-activated state of tropomyosin on actin filaments. Circ Res. 2010;106:705-11 pubmed publisher
    Ca(2+) control of troponin-tropomyosin position on actin regulates cardiac muscle contraction...
  24. Asumda F, Chase P. Nuclear cardiac troponin and tropomyosin are expressed early in cardiac differentiation of rat mesenchymal stem cells. Differentiation. 2012;83:106-15 pubmed publisher
    ..We first tested the hypothesis that nuclear actin and cardiac isoforms of troponin C (cTnC) and tropomyosin (cTm) are present along with cTnI and cTnT in nuclei of isolated, neonatal rat cardiomyocytes in culture...
  25. Fath T, Agnes Chan Y, Vrhovski B, Clarke H, Curthoys N, Hook J, et al. New aspects of tropomyosin-regulated neuritogenesis revealed by the deletion of Tm5NM1 and 2. Eur J Cell Biol. 2010;89:489-98 pubmed publisher
    ..Although functional redundancy does not exist between tropomyosin genes, these results suggest that significant redundancy exists between products from the same gene.
  26. Li X, Lehman W, Fischer S, Holmes K. Curvature variation along the tropomyosin molecule. J Struct Biol. 2010;170:307-12 pubmed publisher
    Complementarity between the tropomyosin supercoil and the helical contour of actin-filaments is required for the binding interaction of actin and tropomyosin (Li et al., 2010)...
  27. Han J, Pollak J, Yang T, Siddiqui M, Doyle K, Taravosh Lahn K, et al. Delayed administration of a small molecule tropomyosin-related kinase B ligand promotes recovery after hypoxic-ischemic stroke. Stroke. 2012;43:1918-24 pubmed publisher
    ..It binds to both the tropomyosin-related kinase B and p75 neurotrophin receptors...
  28. Hasegawa Y, Suzuki H, Altay O, Zhang J. Preservation of tropomyosin-related kinase B (TrkB) signaling by sodium orthovanadate attenuates early brain injury after subarachnoid hemorrhage in rats. Stroke. 2011;42:477-83 pubmed publisher
    ..factor, insulin-like growth factor-1, and vascular endothelial growth factor and phosphorylation of tropomyosin-related kinase B, which is a receptor tyrosine kinase for brain-derived neurotrophic factor and the downstream ..
  29. Brown J. How sequence directs bending in tropomyosin and other two-stranded alpha-helical coiled coils. Protein Sci. 2010;19:1366-75 pubmed publisher
    ..in the axial staggering of the coiled coil, occurring at the boundaries of either clusters of core alanines in tropomyosin or of clusters of core bulky residues in the myosin rod, causes bending within the plane of the local dimer...
  30. Scellini B, Piroddi N, Poggesi C, Tesi C. Extraction and replacement of the tropomyosin-troponin complex in isolated myofibrils. Adv Exp Med Biol. 2010;682:163-74 pubmed publisher
    b>Tropomyosin (Tm) is an essential component in the regulation of striated muscle contraction. Questions about Tm functional role have been difficult to study because sarcomere Tm content is not as easily manipulated as Troponin (Tn)...
  31. Ulke Lemee A, Ishida H, Borman M, Valderrama A, Vogel H, MacDonald J. Tropomyosin-binding properties of the CHASM protein are dependent upon its calponin homology domain. FEBS Lett. 2010;584:3311-6 pubmed publisher
    ..In this study, we demonstrate an interaction between CHASM and tropomyosin. Deletion constructs of CHASM were generated, and pull-down assays revealed a minimal deletion construct that ..
  32. Li X, Holmes K, Lehman W, Jung H, Fischer S. The shape and flexibility of tropomyosin coiled coils: implications for actin filament assembly and regulation. J Mol Biol. 2010;395:327-39 pubmed publisher
    Wrapped superhelically around actin filaments, the coiled-coil alpha-helices of tropomyosin regulate muscle contraction by cooperatively blocking or exposing myosin-binding sites on actin...
  33. Rajan S, Jagatheesan G, Karam C, Alves M, Bodi I, Schwartz A, et al. Molecular and functional characterization of a novel cardiac-specific human tropomyosin isoform. Circulation. 2010;121:410-8 pubmed publisher
    b>Tropomyosin (TM), an essential actin-binding protein, is central to the control of calcium-regulated striated muscle contraction...
  34. Barua B, Pamula M, Hitchcock Degregori S. Evolutionarily conserved surface residues constitute actin binding sites of tropomyosin. Proc Natl Acad Sci U S A. 2011;108:10150-5 pubmed publisher
    b>Tropomyosin (Tm) is a two-chained, ?-helical coiled-coil protein that associates end-to-end to form a continuous strand along actin filaments and regulates the functions and stability of actin in eukaryotic muscle and nonmuscle cells...
  35. Ravkov E, Pavlov I, Martins T, Gleich G, Wagner L, Hill H, et al. Identification and validation of shrimp-tropomyosin specific CD4 T cell epitopes. Hum Immunol. 2013;74:1542-9 pubmed publisher
    ..We sought to identify and validate CD4 T cell shrimp tropomyosin-derived epitopes and characterize CD4 T cell responses in subjects with a clinical history of shellfish allergy...
  36. Uversky V, Shah S, Gritsyna Y, Hitchcock Degregori S, Kostyukova A. Systematic analysis of tropomodulin/tropomyosin interactions uncovers fine-tuned binding specificity of intrinsically disordered proteins. J Mol Recognit. 2011;24:647-55 pubmed publisher
    ..In this study we undertook a systematic analysis of the specificity of the tropomodulin (Tmod) interaction with tropomyosin (TM) to show that affinities of different Tmod isoforms to TM are isoform-dependent...
  37. Siththanandan V, Tobacman L, Van Gorder N, Homsher E. Mechanical and kinetic effects of shortened tropomyosin reconstituted into myofibrils. Pflugers Arch. 2009;458:761-76 pubmed publisher
    The effects of tropomyosin on muscle mechanics and kinetics were examined in skeletal myofibrils using a novel method to remove tropomyosin (Tm) and troponin (Tn) and then replace these proteins with altered versions...
  38. Park T, Kang J, Na B, Sohn W. Molecular cloning and characterization of a paramyosin from Clonorchis sinensis. Korean J Parasitol. 2009;47:359-67 pubmed publisher
    ..Successful induction of host IgG production also suggests that CsPmy can be applied as a diagnostic antigen and/or vaccine candidate for clonorchiasis...
  39. Li X, Lehman W, Fischer S. The relationship between curvature, flexibility and persistence length in the tropomyosin coiled-coil. J Struct Biol. 2010;170:313-8 pubmed publisher
    The inherent flexibility of rod-like tropomyosin coiled-coils is a significant factor that constrains tropomyosin's complex positional dynamics on actin filaments...
  40. Bugyi B, Didry D, Carlier M. How tropomyosin regulates lamellipodial actin-based motility: a combined biochemical and reconstituted motility approach. EMBO J. 2010;29:14-26 pubmed publisher
    ..b>Tropomyosin severely inhibits lamellipodium formation and facilitates the lamellar array while enhancing migration, by a ..
  41. Song W, Dyer E, Stuckey D, Leung M, Memo M, Mansfield C, et al. Investigation of a transgenic mouse model of familial dilated cardiomyopathy. J Mol Cell Cardiol. 2010;49:380-9 pubmed publisher
    ..F-actin isolated from ACTC E361G mouse hearts was incorporated into thin filaments with native human tropomyosin and troponin and compared with NTG mouse actin by in vitro motility assay...
  42. Bach C, Schevzov G, Bryce N, Gunning P, O Neill G. Tropomyosin isoform modulation of focal adhesion structure and cell migration. Cell Adh Migr. 2010;4:226-34 pubmed
    ..The tropomyosin family of actin-associating proteins are emerging as important modulators of the contractile nature of ..
  43. Barua B, Fagnant P, Winkelmann D, Trybus K, Hitchcock Degregori S. A periodic pattern of evolutionarily conserved basic and acidic residues constitutes the binding interface of actin-tropomyosin. J Biol Chem. 2013;288:9602-9 pubmed publisher
    ..A universal actin filament regulator is the protein tropomyosin, which binds end-to-end along the length of the filament...
  44. Karam C, Warren C, Rajan S, de Tombe P, Wieczorek D, Solaro R. Expression of tropomyosin-? induces dilated cardiomyopathy and depresses cardiac myofilament tension by mechanisms involving cross-bridge dependent activation and altered tropomyosin phosphorylation. J Muscle Res Cell Motil. 2011;31:315-22 pubmed publisher
    b>Tropomyosin-kappa (TPM1-?) is a newly discovered tropomyosin (TM) isoform that is exclusively expressed in the human heart and generated by an alternative splicing of the ?-TM gene...
  45. Wang F, Brunet N, Grubich J, Bienkiewicz E, Asbury T, Compton L, et al. Facilitated cross-bridge interactions with thin filaments by familial hypertrophic cardiomyopathy mutations in ?-tropomyosin. J Biomed Biotechnol. 2011;2011:435271 pubmed publisher
    ..When HMM density was reduced, thin filaments with D175N required fewer motors to initiate sliding or achieve maximum sliding speed. ..
  46. Hodges A, Krementsova E, Bookwalter C, Fagnant P, Sladewski T, Trybus K. Tropomyosin is essential for processive movement of a class V myosin from budding yeast. Curr Biol. 2012;22:1410-6 pubmed publisher
    ..The key to processivity is tropomyosin: Myo2p is not processive on bare actin but highly processive on actin-tropomyosin...
  47. Kubo E, Hasanova N, Fatma N, Sasaki H, Singh D. Elevated tropomyosin expression is associated with epithelial-mesenchymal transition of lens epithelial cells. J Cell Mol Med. 2013;17:212-21 pubmed publisher
    ..The tropomyosin (Tpm) family of cytoskeleton proteins is involved in regulating and stabilizing actin microfilaments...
  48. Masuda S, Tomohiro T, Hatanaka Y. Rapidly photoactivatable ATP probes for specific labeling of tropomyosin within the actomyosin protein complex. Bioorg Med Chem Lett. 2011;21:2252-4 pubmed publisher
    b>Tropomyosin-specific photoaffinity adenosine triphosphate (ATP) probes have been first developed, in which a diazirine moiety is incorporated into the ?-phosphate group as a rapidly carbene-generating photophore...
  49. Yang A, Arruda L, Santos A, Barbosa M, Chapman M, Galvão C, et al. Measurement of IgE antibodies to shrimp tropomyosin is superior to skin prick testing with commercial extract and measurement of IgE to shrimp for predicting clinically relevant allergic reactions after shrimp ingestion. J Allergy Clin Immunol. 2010;125:872-8 pubmed publisher
    Shrimp is a frequent cause of food allergy. Tropomyosin is the major allergen in shrimp, and it shares homology to tropomyosins from other crustaceans, dust mites, cockroach, and parasites...
  50. Loong C, Zhou H, Chase P. Persistence length of human cardiac ?-tropomyosin measured by single molecule direct probe microscopy. PLoS ONE. 2012;7:e39676 pubmed publisher
    b>Tropomyosin (?Tm) is the predominant tropomyosin isoform in adult human heart and constitutes a major component in Ca²+-regulated systolic contraction of cardiac muscle...
  51. Lehman W, Galinska Rakoczy A, Hatch V, Tobacman L, Craig R. Structural basis for the activation of muscle contraction by troponin and tropomyosin. J Mol Biol. 2009;388:673-81 pubmed publisher
    ..striated muscle contraction couples the binding and dissociation of Ca(2+) on troponin (Tn) to the movement of tropomyosin on actin filaments...
  52. Choi C, Kim D, Kim S, Jeong S, Song E, Helfman D. From skeletal muscle to cancer: insights learned elucidating the function of tropomyosin. J Struct Biol. 2012;177:63-9 pubmed publisher
    ..In multicellular organisms they exhibit extensive cell type specific isoform diversity. In this essay we discuss the genetic mechanisms by which this diversity is generated and its significance to actin-based cellular functions. ..
  53. Liu R, Holck A, Yang E, Liu C, Xue W. Tropomyosin from tilapia (Oreochromis mossambicus) as an allergen. Clin Exp Allergy. 2013;43:365-77 pubmed publisher
    ..Tilapia is among the most common fresh water fish species raised by fish farms and can cause allergic reactions upon ingestion...
  54. Ali L, Cohen J, Tobacman L. Push and pull of tropomyosin's opposite effects on myosin attachment to actin. A chimeric tropomyosin host-guest study. Biochemistry. 2010;49:10873-80 pubmed publisher
    b>Tropomyosin is a ubiquitous actin-binding protein with an extended coiled-coil structure. Tropomyosin-actin interactions are weak and loosely specific, but they potently influence myosin...
  55. Mathur M, Chase P, Chalovich J. Several cardiomyopathy causing mutations on tropomyosin either destabilize the active state of actomyosin or alter the binding properties of tropomyosin. Biochem Biophys Res Commun. 2011;406:74-8 pubmed publisher
    We examined the cardiomyopathy-causing tropomyosin mutations E180G, D175N, and V95A to determine their effects on actomyosin regulation...
  56. Behrmann E, Müller M, Penczek P, Mannherz H, Manstein D, Raunser S. Structure of the rigor actin-tropomyosin-myosin complex. Cell. 2012;150:327-38 pubmed publisher
    Regulation of myosin and filamentous actin interaction by tropomyosin is a central feature of contractile events in muscle and nonmuscle cells...
  57. Clayton J, Sammons M, Stark B, Hodges A, Lord M. Differential regulation of unconventional fission yeast myosins via the actin track. Curr Biol. 2010;20:1423-31 pubmed publisher
    ..However, decoration of actin with tropomyosin differentially regulates the activity of these motors...
  58. Creed S, Desouza M, Bamburg J, Gunning P, Stehn J. Tropomyosin isoform 3 promotes the formation of filopodia by regulating the recruitment of actin-binding proteins to actin filaments. Exp Cell Res. 2011;317:249-61 pubmed publisher
    ..However, accumulating evidence suggests that fundamental differences in function exist between tropomyosin isoforms, which contributes to the formation of functionally distinct filament populations...
  59. Li X, Suphamungmee W, Janco M, Geeves M, Marston S, Fischer S, et al. The flexibility of two tropomyosin mutants, D175N and E180G, that cause hypertrophic cardiomyopathy. Biochem Biophys Res Commun. 2012;424:493-6 pubmed publisher
    ..to elucidate molecular defects leading to cardiac dysfunction, we have examined the structural mechanics of two tropomyosin mutants, E180G and D175N, which are associated with hypertrophic cardiomyopathy (HCM)...
  60. Rao J, Rivera Santiago R, Li X, Lehman W, Dominguez R. Structural analysis of smooth muscle tropomyosin ? and ? isoforms. J Biol Chem. 2012;287:3165-74 pubmed publisher
    A large number of tropomyosin (Tm) isoforms function as gatekeepers of the actin filament, controlling the spatiotemporal access of actin-binding proteins to specialized actin networks...
  61. Clarke N, Domazetovska A, Waddell L, Kornberg A, McLean C, North K. Cap disease due to mutation of the beta-tropomyosin gene (TPM2). Neuromuscul Disord. 2009;19:348-51 pubmed publisher
    ..Mutation of the TPM2 gene, that encodes beta-tropomyosin, is the first reported genetic cause...
  62. Ostap E. Tropomyosins as discriminators of myosin function. Adv Exp Med Biol. 2008;644:273-82 pubmed
    Vertebrate nonmuscle cells express multiple tropomyosin isoforms that are sorted to subcellular compartments that have distinct morphological and dynamic properties...
  63. Monasky M, Biesiadecki B, Janssen P. Increased phosphorylation of tropomyosin, troponin I, and myosin light chain-2 after stretch in rabbit ventricular myocardium under physiological conditions. J Mol Cell Cardiol. 2010;48:1023-8 pubmed publisher
    ..Pro-Q diamond staining as well as phosphorylation-specific antibodies revealed increased phosphorylation of tropomyosin, troponin I, and myosin light chain-2 at longer muscle lengths...
  64. Ueda K, Kimura Sakiyama C, Aihara T, Miki M, Arata T. Interaction sites of tropomyosin in muscle thin filament as identified by site-directed spin-labeling. Biophys J. 2011;100:2432-9 pubmed publisher
    ..label covalently bound to the side chain of a cysteine genetically incorporated into rabbit skeletal muscle tropomyosin (Tm) at positions 13, 36, 146, 160, 174, 190, 209, 230, 271, and 279...
  65. Cherny D, Gooding C, Eperon G, Coelho M, Bagshaw C, Smith C, et al. Stoichiometry of a regulatory splicing complex revealed by single-molecule analyses. EMBO J. 2010;29:2161-72 pubmed publisher
    ..Polypyrimidine tract-binding protein (PTB), which regulates tissue-specific splicing, represses exon 3 of alpha-tropomyosin through distant pyrimidine-rich tracts in the flanking introns...
  66. Erban T. Purification of tropomyosin, paramyosin, actin, tubulin, troponin and kinases for chemiproteomics and its application to different scientific fields. PLoS ONE. 2011;6:e22860 pubmed publisher
    ..A p-Aminobenzamidine (p-ABA) ligand enabled the purification of the panallergenic proteins tropomyosin and paramyosin, as well as actin, tubulin, troponin and several kinases and annexins, with variable specificity ..
  67. Wang M, Li W, Chang G, Ye C, Ou J, Li X, et al. MicroRNA-21 regulates vascular smooth muscle cell function via targeting tropomyosin 1 in arteriosclerosis obliterans of lower extremities. Arterioscler Thromb Vasc Biol. 2011;31:2044-53 pubmed publisher
    ..3'-Untranslated region luciferase assay confirmed that tropomyosin 1 was a target of miR-21 that was involved in miR-21-mediated cellular effects, such as cell shape modulation...
  68. Zhang C, Jia P, Huang X, Sferrazza G, Athauda G, Achary M, et al. Myofibril-inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts. J Biomed Sci. 2009;16:81 pubmed publisher
    ..The present study demonstrates that the MIR gene is essential for tropomyosin (TM) expression in axolotl hearts during development...
  69. Huang B, Chen S, Wang D. Shear flow increases S-nitrosylation of proteins in endothelial cells. Cardiovasc Res. 2009;83:536-46 pubmed publisher
    ..The S-nitrosylated residues in tropomyosin and vimentin, which were localized in the hydrophobic motif of each protein, were identified as Cys170 and ..
  70. Hitchcock Degregori S, Singh A. What makes tropomyosin an actin binding protein? A perspective. J Struct Biol. 2010;170:319-24 pubmed publisher
    b>Tropomyosin is a two-chained alpha-helical coiled coil that binds along the length of the actin filament and regulates its function...
  71. Geeves M, Griffiths H, MIJAILOVICH S, Smith D. Cooperative [Ca²+]-dependent regulation of the rate of myosin binding to actin: solution data and the tropomyosin chain model. Biophys J. 2011;100:2679-87 pubmed publisher
    The regulation of muscle contraction by calcium involves interactions among actin filaments, myosin-S1, tropomyosin (Tm), and troponin (Tn)...
  72. Yamashiro S, Gokhin D, Sui Z, Bergeron S, Rubenstein P, Fowler V. Differential actin-regulatory activities of Tropomodulin1 and Tropomodulin3 with diverse tropomyosin and actin isoforms. J Biol Chem. 2014;289:11616-29 pubmed publisher
    ..The ability of Tmod3 to sequester ?- and ?cyto-actin (but not ?sk-actin) monomers in the absence of TMs suggests a novel function for Tmod3 in regulating actin remodeling or turnover in cells. ..
  73. Bai F, Weis A, Takeda A, Chase P, Kawai M. Enhanced active cross-bridges during diastole: molecular pathogenesis of tropomyosin's HCM mutations. Biophys J. 2011;100:1014-23 pubmed publisher
    Three HCM-causing tropomyosin (Tm) mutants (V95A, D175N, and E180G) were examined using the thin-filament extraction and reconstitution technique...
  74. Tang H, Beer L, Tanyi J, Zhang R, Liu Q, Speicher D. Protein isoform-specific validation defines multiple chloride intracellular channel and tropomyosin isoforms as serological biomarkers of ovarian cancer. J Proteomics. 2013;89:165-78 pubmed publisher
    ..LC-MS/MS analysis to determine the members of two protein families, chloride intracellular channel (CLIC) and tropomyosin (TPM) proteins that are detectable in sera of ovarian cancer patients...
  75. Wang W, Qi C, Kang T, Niu Y, Jin G, Ge Y, et al. Analysis of the interaction between tropomyosin allergens and antibodies using a biosensor based on imaging ellipsometry. Anal Chem. 2013;85:4446-52 pubmed publisher
    ..resolution imaging ellipsometry has been studied to examine its role in the rapid detection and analysis of the tropomyosin allergen existing in crustaceans...
  76. Karpicheva O, Redwood C, Borovikov Y. The E117K mutation in ?-tropomyosin disturbs concerted conformational changes of actomyosin in muscle fibers. Arch Biochem Biophys. 2014;549:12-6 pubmed publisher
    The effect of the skeletal myopathy-causing E117K mutation in human ?-tropomyosin on actomyosin structure during the ATPase cycle was studied using fluorescent probes bound to actin subdomain 1 and the myosin head...
  77. Ye T, Zong R, Zhang X. Involvement of interaction between viral VP466 and host tropomyosin proteins in virus infection in shrimp. Gene. 2012;505:254-8 pubmed publisher
    ..It was found that the VP466 protein was interacted with the host cytoskeleton protein tropomyosin. As documented, the VP466-tropomyosin interaction facilitated the WSSV infection...
  78. Becker S, Gröger M, Canis M, Pfrogner E, Kramer M. Tropomyosin sensitization in house dust mite allergic patients. Eur Arch Otorhinolaryngol. 2012;269:1291-6 pubmed publisher
    ..b>Tropomyosin, the major shellfish allergen is regarded to be responsible for clinical cross-reactivity to inhaled house dust ..
  79. Frye J, Klenchin V, Rayment I. Structure of the tropomyosin overlap complex from chicken smooth muscle: insight into the diversity of N-terminal recognition. Biochemistry. 2010;49:4908-20 pubmed publisher
    b>Tropomyosin is a stereotypical alpha-helical coiled coil that polymerizes to form a filamentous macromolecular assembly that lies on the surface of F-actin...
  80. Li X, Tobacman L, Mun J, Craig R, Fischer S, Lehman W. Tropomyosin position on F-actin revealed by EM reconstruction and computational chemistry. Biophys J. 2011;100:1005-13 pubmed publisher
    Electron microscopy and fiber diffraction studies of reconstituted F-actin-tropomyosin filaments reveal the azimuthal position of end-to-end linked tropomyosin molecules on the surface of actin...
  81. Ochala J, Gokhin D, Penisson Besnier I, Quijano Roy S, Monnier N, Lunardi J, et al. Congenital myopathy-causing tropomyosin mutations induce thin filament dysfunction via distinct physiological mechanisms. Hum Mol Genet. 2012;21:4473-85 pubmed
    In humans, congenital myopathy-linked tropomyosin mutations lead to skeletal muscle dysfunction, but the cellular and molecular mechanisms underlying such dysfunction remain obscure...
  82. Mudalige W, Tao T, Lehrer S. Ca2+-dependent photocrosslinking of tropomyosin residue 146 to residues 157-163 in the C-terminal domain of troponin I in reconstituted skeletal muscle thin filaments. J Mol Biol. 2009;389:575-83 pubmed publisher
    The Ca(2+)-dependent interaction of troponin I (TnI) with actin.tropomyosin (Tm) in muscle thin filaments is a critical step in the regulation of muscle contraction...
  83. Tajsharghi H, Ohlsson M, Palm L, Oldfors A. Myopathies associated with ?-tropomyosin mutations. Neuromuscul Disord. 2012;22:923-33 pubmed publisher
    Mutations in TPM2, encoding ?-tropomyosin, have recently been found to cause a range of muscle disorders...
  84. Jagatheesan G, Rajan S, Wieczorek D. Investigations into tropomyosin function using mouse models. J Mol Cell Cardiol. 2010;48:893-8 pubmed publisher
    b>Tropomyosin plays a key role in controlling calcium regulated sarcomeric contraction through its interactions with actin and the troponin complex...
  85. Miki M, Makimura S, Saitoh T, Bunya M, Sugahara Y, Ueno Y, et al. A three-dimensional FRET analysis to construct an atomic model of the actin-tropomyosin complex on a reconstituted thin filament. J Mol Biol. 2011;414:765-82 pubmed publisher
    Fluorescence resonance energy transfer (FRET) was used to construct an atomic model of the actin-tropomyosin (Tm) complex on a reconstituted thin filament...
  86. Zhang Z, Akhter S, Mottl S, Jin J. Calcium-regulated conformational change in the C-terminal end segment of troponin I and its binding to tropomyosin. FEBS J. 2011;278:3348-59 pubmed publisher
    ..Reconstituted troponin complex containing a mini-troponin T lacking its two tropomyosin-binding sites showed a saturable binding to tropomyosin at pCa 9 but not at pCa 4...