smooth muscle myosins


Summary: Myosin type II isoforms found in smooth muscle.

Top Publications

  1. Kim H, Kojima K, Swindle C, Cotta C, Huo Y, Reddy V, et al. FLT3-ITD cooperates with inv(16) to promote progression to acute myeloid leukemia. Blood. 2008;111:1567-74 pubmed
    ..These results indicate that FLT3-activating mutations can cooperate with CBFbeta-SMMHC in an animal model of inv(16)-associated AML. ..
  2. Babu G, Loukianov E, Loukianova T, Pyne G, Huke S, Osol G, et al. Loss of SM-B myosin affects muscle shortening velocity and maximal force development. Nat Cell Biol. 2001;3:1025-9 pubmed
    ..We conclude that the extra seven-residue insert in the surface loop 1 of SM-B myosin is a critical determinant of crossbridge cycling and velocity of shortening. ..
  3. Wanjare M, Kuo F, Gerecht S. Derivation and maturation of synthetic and contractile vascular smooth muscle cells from human pluripotent stem cells. Cardiovasc Res. 2013;97:321-30 pubmed publisher
    ..Deriving Con-vSMCs from an integration-free hiPSC line may prove useful for regenerative therapy involving blood vessel differentiation and stabilization. ..
  4. Rovner A, Fagnant P, Trybus K. Phosphorylation of a single head of smooth muscle myosin activates the whole molecule. Biochemistry. 2006;45:5280-9 pubmed
    ..2003) J. Mol. Biol. 329, 963-972], allowing activation of both the phosphorylated and dephosphorylated heads. These findings help explain how smooth muscles are able to generate high levels of stress with low phosphorylation levels. ..
  5. Yin F, Hoggatt A, Zhou J, Herring B. 130-kDa smooth muscle myosin light chain kinase is transcribed from a CArG-dependent, internal promoter within the mouse mylk gene. Am J Physiol Cell Physiol. 2006;290:C1599-609 pubmed
    ..Altogether, these data suggest that SRF and SRF-associated factors play a key role in regulating the expression of smMLCK. ..
  6. Arner A, Löfgren M, Morano I. Smooth, slow and smart muscle motors. J Muscle Res Cell Motil. 2003;24:165-73 pubmed
    ..Non-muscle myosin has been shown to have a contractile function in some smooth muscle tissues and recent data on the kinetic properties of non-muscle myosin filaments in smooth muscle tissue are described. ..
  7. Zhang H, Ji H, Ni T, Ma R, Wang A, Li X. Characterization of Blebbistatin Inhibition of Smooth Muscle Myosin and Nonmuscle Myosin-2. Biochemistry. 2017;56:4235-4243 pubmed publisher
  8. Hong F, Facemyer K, Carter M, Jackson D, Haldeman B, Ruana N, et al. Kinetics of myosin light chain kinase activation of smooth muscle myosin in an in vitro model system. Biochemistry. 2013;52:8489-500 pubmed publisher
    ..This would avoid sequestering MLCK to unproductive interactions with previously phosphorylated SMM, potentially leading to faster rates of phosphorylation in muscle. ..
  9. Vickaryous N, Polanco Echeverry G, Morrow S, Suraweera N, Thomas H, Tomlinson I, et al. Smooth-muscle myosin mutations in hereditary non-polyposis colorectal cancer syndrome. Br J Cancer. 2008;99:1726-8 pubmed publisher
    ..Our results show that mutations in MYH11 occur more frequently in cancers than adenomas (P=0.008) and are dependent on microsatellite instability (MSI+). ..

More Information


  1. Khromov A, Webb M, Ferenczi M, Trentham D, Somlyo A, Somlyo A. Myosin regulatory light chain phosphorylation and strain modulate adenosine diphosphate release from smooth muscle Myosin. Biophys J. 2004;86:2318-28 pubmed
    ..ADP state(s) and contributes to the high economy of force. ..
  2. Yang J, Feng X, Zhang Y, Lin Y. Influence of trace amount of calponin on smooth muscle myosin in different states. Acta Biochim Biophys Sin (Shanghai). 2004;36:529-36 pubmed
    ..However, why and how TAC can possess such a high efficiency to influence myosin and how the physiological significance of the high efficiency of TAC is in regulating the interaction between myosin and actin remain to be investigated. ..
  3. Haraguchi T, Honda K, Wanikawa Y, Shoji N, Yamamoto K, Ito K. Function of the head-tail junction in the activity of myosin II. Biochem Biophys Res Commun. 2013;440:490-4 pubmed publisher
    ..Our results suggest that the first leucine of the conserved Leu-Leu sequence at the head-tail junction profoundly affects the cooperativity between the two heads involved in the actin activated ATPase activity of myosin II. ..
  4. Sheng S, Gao Y, Khromov A, Somlyo A, Somlyo A, Shao Z. Cryo-atomic force microscopy of unphosphorylated and thiophosphorylated single smooth muscle myosin molecules. J Biol Chem. 2003;278:39892-6 pubmed
  5. Ni S, Hong F, Brewer P, Ikebe M, Onishi H, BAKER J, et al. Kinetic and motor functions mediated by distinct regions of the regulatory light chain of smooth muscle myosin. Biochim Biophys Acta. 2009;1794:1599-605 pubmed publisher
  6. Mao X, Debenedittis P, Sun Y, Chen J, Yuan K, Jiao K, et al. Vascular smooth muscle cell Smad4 gene is important for mouse vascular development. Arterioscler Thromb Vasc Biol. 2012;32:2171-7 pubmed publisher
    ..These studies provide important insight into the role of Smad4 and its upstream Smads in regulating SMC function and vascular development of mice. ..
  7. Babu G, Pyne G, Zhou Y, Okwuchukuasanya C, Brayden J, Osol G, et al. Isoform switching from SM-B to SM-A myosin results in decreased contractility and altered expression of thin filament regulatory proteins. Am J Physiol Cell Physiol. 2004;287:C723-9 pubmed
    ..These results suggest that the presence or absence of SM-B myosin is a critical determinant of smooth muscle contraction and that its loss leads to additional changes in thin filament regulatory proteins. ..
  8. Kalof A, Tam D, Beatty B, Cooper K. Immunostaining patterns of myoepithelial cells in breast lesions: a comparison of CD10 and smooth muscle myosin heavy chain. J Clin Pathol. 2004;57:625-9 pubmed
    ..Although CD10 can aid in the distinction between IC and DCIS, SMMHC is a more sensitive and specific marker of MEC and shows less heterogeneity of immunostaining patterns. ..
  9. Ohki T, Mikhailenko S, Morales M, Onishi H, Mochizuki N. Transmission of force and displacement within the myosin molecule. Biochemistry. 2004;43:13707-14 pubmed
    ..From interpreting our observations in terms of the structure of the converter, we deduce that the linear-rotational transformation in the converter is mediated by a little machine (two Phe residues linked to a Gly) within a machine. ..
  10. Bhetwal B, An C, Fisher S, Perrino B. Regulation of basal LC20 phosphorylation by MYPT1 and CPI-17 in murine gastric antrum, gastric fundus, and proximal colon smooth muscles. Neurogastroenterol Motil. 2011;23:e425-36 pubmed publisher
    ..Organ-specific mechanisms involving the MLCP interacting proteins LZ +/- MYPT1, M-RIP, and CPI-17 are critical to regulating basal LC20 phosphorylation in gastrointestinal smooth muscles. ..
  11. Gorczynski M, Grembecka J, Zhou Y, Kong Y, Roudaia L, Douvas M, et al. Allosteric inhibition of the protein-protein interaction between the leukemia-associated proteins Runx1 and CBFbeta. Chem Biol. 2007;14:1186-97 pubmed
    ..Treatment of the human leukemia cell line ME-1 with these compounds shows decreased proliferation, indicating these are good candidates for further development. ..
  12. Leguillette R, Lauzon A. Molecular mechanics of smooth muscle contractile proteins in airway hyperresponsiveness and asthma. Proc Am Thorac Soc. 2008;5:40-6 pubmed
    ..Further considerations are given to the combination of these two factors with other components of the contractile machinery, thereby leading to the enhancement of airway SM function. ..
  13. Zhang Y, Nakamura A, Kawamichi H, Yoshiyama S, Katayama T, Kohama K. Calcium regulation of the ATPase activity of Physarum and scallop myosins using hybrid smooth muscle myosin: the role of the essential light chain. FEBS Lett. 2010;584:3486-91 pubmed publisher
    ..Our results suggest that communication between the original combinations of LC is important for the motor function. ..
  14. Jung H, Billington N, Thirumurugan K, Salzameda B, Cremo C, Chalovich J, et al. Role of the tail in the regulated state of myosin 2. J Mol Biol. 2011;408:863-78 pubmed publisher
    ..Our results support the view that interactions between the heads and the distal tail perform a critical role in regulating activity of myosin 2 molecules through stabilizing the compact monomer conformation. ..
  15. Siddiqui S, Novali M, Tsuchiya K, Hirota N, Geller B, McGovern T, et al. The modulation of large airway smooth muscle phenotype and effects of epidermal growth factor receptor inhibition in the repeatedly allergen-challenged rat. Am J Physiol Lung Cell Mol Physiol. 2013;304:L853-62 pubmed publisher
    ..Furthermore, the latter results in excessive small airway and, to a lesser degree, large airway narrowing to methacholine, and large airway gene expression of contractile protein is conserved. ..
  16. Grassie M, Sutherland C, Ulke Lemee A, Chappellaz M, Kiss E, Walsh M, et al. Cross-talk between Rho-associated kinase and cyclic nucleotide-dependent kinase signaling pathways in the regulation of smooth muscle myosin light chain phosphatase. J Biol Chem. 2012;287:36356-69 pubmed publisher
  17. Walcott S, Warshaw D. Modeling smooth muscle myosin's two heads: long-lived enzymatic roles and phosphorylation-dependent equilibria. Biophys J. 2010;99:1129-38 pubmed publisher
  18. Zhang X, Kanika N, Melman A, Disanto M. Smooth muscle myosin expression, isoform composition, and functional activities in rat corpus cavernosum altered by the streptozotocin-induced type 1 diabetes. Am J Physiol Endocrinol Metab. 2012;302:E32-42 pubmed publisher
    ..Moreover, the change in CCSM phenotype was associated with an increased sensitivity to BLEB, which may serve as a novel pharmacotherapy for ED. ..
  19. Saad R, Liu Y, Silverman J. Distribution of basal/myoepithelial markers in benign and malignant bronchioloalveolar proliferations of the lung. Appl Immunohistochem Mol Morphol. 2010;18:219-25 pubmed publisher
    ..The staining pattern of basal cells in bronchioloalveolar carcinoma supports that these neoplasms may actually be carcinoma in-situ. ..
  20. Volkmann N, Hanein D. Docking of atomic models into reconstructions from electron microscopy. Methods Enzymol. 2003;374:204-25 pubmed
  21. Martin A, Bhatti S, Pyne Geithman G, Farjah M, Manaves V, Walker L, et al. Expression and function of COOH-terminal myosin heavy chain isoforms in mouse smooth muscle. Am J Physiol Cell Physiol. 2007;293:C238-45 pubmed
    ..These data provide evidence indicating an in vivo function for the COOH-terminal isoforms of smooth muscle myosin and suggest that the SM1:SM2 ratio is tightly regulated in smooth muscle tissues. ..
  22. Itoh S, Umemoto S, Hiromoto M, Toma Y, Tomochika Y, Aoyagi S, et al. Importance of NAD(P)H oxidase-mediated oxidative stress and contractile type smooth muscle myosin heavy chain SM2 at the early stage of atherosclerosis. Circulation. 2002;105:2288-95 pubmed
  23. Alcala D, Haldeman B, Brizendine R, Krenc A, Baker J, Rock R, et al. Myosin light chain kinase steady-state kinetics: comparison of smooth muscle myosin II and nonmuscle myosin IIB as substrates. Cell Biochem Funct. 2016;34:469-474 pubmed publisher
    ..We show that the MLCK prefers smooth muscle myosin by a significant factor. These data suggest that nonmuscle myosin is phosphorylated more slowly than smooth muscle myosin during a contraction cycle. ..
  24. Schaafsma D, McNeill K, Stelmack G, Gosens R, Baarsma H, Dekkers B, et al. Insulin increases the expression of contractile phenotypic markers in airway smooth muscle. Am J Physiol Cell Physiol. 2007;293:C429-39 pubmed
    ..This could have important implications for the use of recently approved aerosolized insulin formulations in diabetes mellitus. ..
  25. Alhopuro P, Phichith D, Tuupanen S, Sammalkorpi H, Nybondas M, Saharinen J, et al. Unregulated smooth-muscle myosin in human intestinal neoplasia. Proc Natl Acad Sci U S A. 2008;105:5513-8 pubmed publisher
    ..These data challenge our view on MYH11 as a passive differentiation marker functioning in muscle contraction and add to our understanding of intestinal neoplasia. ..
  26. Iorga B, Adamek N, Geeves M. The slow skeletal muscle isoform of myosin shows kinetic features common to smooth and non-muscle myosins. J Biol Chem. 2007;282:3559-70 pubmed
    ..The role of these actin-myosin.ADP complexes in the mechanochemistry of slow muscle contraction is discussed in relation to the load dependence of ADP release...
  27. Decarreau J, Chrin L, Berger C. Loop 1 dynamics in smooth muscle myosin: isoform specific differences modulate ADP release. J Muscle Res Cell Motil. 2011;32:49-61 pubmed publisher
    ..Thus movement of Loop 1 is a critical factor in increasing the ADP release rate in the inserted faster isoform of SM myosin. ..
  28. Espinoza Fonseca L, Kast D, Thomas D. Molecular dynamics simulations reveal a disorder-to-order transition on phosphorylation of smooth muscle myosin. Biophys J. 2007;93:2083-90 pubmed
    ..The simulations provide new insight into structural details not revealed by experiment, allowing us to propose a refined model for the mechanism by which phosphorylation affects the N-terminal domain of the RLC of smooth muscle myosin. ..
  29. Quarck R, Wynants M, Ronisz A, Sepúlveda M, Wuytack F, Van Raemdonck D, et al. Characterization of proximal pulmonary arterial cells from chronic thromboembolic pulmonary hypertension patients. Respir Res. 2012;13:27 pubmed publisher
    ..Modified proliferative and/or migratory responses of PASMC and PAEC in vitro, associated to a proliferative phenotype of PASMC suggest that PASMC and PAEC could contribute to proximal vascular remodeling in CTEPH. ..
  30. Sakai H, Kurihara Y, Hashimoto Y, Chiba Y, Misawa M. Phorbol 12,13-dibutyrate-mediated contraction of the stimulated bronchial smooth muscles of mice. Respir Physiol Neurobiol. 2010;173:120-4 pubmed publisher
    ..Thus, a certain level of intracellular Ca(2+) might be needed both for an activation of CPI-17 and an induction of contraction induced by PDBu in mouse BSMs. ..
  31. Gil F, Lauzon A. Smooth muscle molecular mechanics in airway hyperresponsiveness and asthma. Can J Physiol Pharmacol. 2007;85:133-40 pubmed
    ..Further studies are therefore required to understand the numerous mechanisms leading to the airway hyperresponsiveness observed in asthma as well as their multiple interactions. ..
  32. Borman M, MacDonald J. Purification of smooth muscle myosin phosphatase using a thiophosphorylated myosin light-chain-affinity resin. Methods Mol Biol. 2007;365:225-33 pubmed
    ..Here we describe the use of thiophosphorylated smooth muscle myosin regulatory light chains for the selective purification of the smooth muscle myosin phosphatase holoenzyme. ..
  33. Zhang H, Tang Z, Yang J, Zhang Y, Li Y, Lin Y. Bi-directional regulation of emodin and quercetin on smooth muscle myosin of gizzard. FEBS Lett. 2006;580:469-73 pubmed
    ..A combination of emodin and quercetin indicates its potential for modulating gastric-intestinal smooth muscle. ..
  34. Uchinaka A, Kawaguchi N, Hamada Y, Mori S, Miyagawa S, Saito A, et al. Transplantation of myoblast sheets that secrete the novel peptide SVVYGLR improves cardiac function in failing hearts. Cardiovasc Res. 2013;99:102-10 pubmed publisher
    ..The SV can induce differentiation of fibroblasts to myofibroblasts via TGF-?-Smad signalling. This peptide could possibly be used as a bridge to heart transplantation or as an ideal peptide drug for cardiac regeneration therapy. ..
  35. Yang Y, Wang W, Cleaves R, Zahurak M, Cheng L, Civin C, et al. Acceleration of G(1) cooperates with core binding factor beta-smooth muscle myosin heavy chain to induce acute leukemia in mice. Cancer Res. 2002;62:2232-5 pubmed
    ..The INV/p16p19 and INV/E7 leukemias were lymphoid and were clonal and retransplantable. Overall, these findings indicate that CBF mutations cooperate with genetic alterations that accelerate G(1) to induce acute leukemia. ..
  36. Chi M, Zhou Y, Vedamoorthyrao S, Babu G, Periasamy M. Ablation of smooth muscle myosin heavy chain SM2 increases smooth muscle contraction and results in postnatal death in mice. Proc Natl Acad Sci U S A. 2008;105:18614-8 pubmed publisher
    ..Also, because SM2(-/-) mice develop lethal multiorgan dysfunctions, we propose this regulatory property of SM2 is essential for normal contractile activity in postnatal smooth muscle physiology. ..
  37. Pucovský V, Moss R, Bolton T. Non-contractile cells with thin processes resembling interstitial cells of Cajal found in the wall of guinea-pig mesenteric arteries. J Physiol. 2003;552:119-33 pubmed
    ..Single AIL cells stained positive for vimentin, desmin and smooth muscle myosin. The presence of ICC-like cells is demonstrated for the first time in the media of resistance arteries. ..
  38. Uranishi R, Baev N, Kim J, Awad I. Vascular smooth muscle cell differentiation in human cerebral vascular malformations. Neurosurgery. 2001;49:671-9; discussion 679-80 pubmed
    ..It is difficult to evaluate VSMC differentiation in culture because of phenotypic change. ..
  39. Watanabe M, Yumoto M, Tanaka H, Wang H, Katayama T, Yoshiyama S, et al. Blebbistatin, a myosin II inhibitor, suppresses contraction and disrupts contractile filaments organization of skinned taenia cecum from guinea pig. Am J Physiol Cell Physiol. 2010;298:C1118-26 pubmed publisher
    ..These results suggested that blebbistatin suppressed skinned smooth muscle contraction through disruption of structure of SMM by the agent...
  40. Hong F, Haldeman B, John O, Brewer P, Wu Y, Ni S, et al. Characterization of tightly associated smooth muscle myosin-myosin light-chain kinase-calmodulin complexes. J Mol Biol. 2009;390:879-92 pubmed publisher
    ..These results suggest that this MLCK may play a role in the initiation of contraction. ..
  41. Ho D, Chen L, Zhao X, Durham N, PANNIRSELVAM M, Vatner D, et al. Smooth muscle myosin inhibition: a novel therapeutic approach for pulmonary hypertension. PLoS ONE. 2012;7:e36302 pubmed publisher
    ..0±3.8%) reduction in right ventricular systolic pressure as compared with sildenafil (20.3±4.5%). Inhibition of smooth muscle myosin may be a novel therapeutic target for treatment of pulmonary hypertension. ..
  42. van Duffelen M, Chrin L, Berger C. Nucleotide dependent intrinsic fluorescence changes of W29 and W36 in smooth muscle myosin. Biophys J. 2004;87:1767-75 pubmed
    ..These results have important implications for the role of the N-terminal domain (residues 1-76) in smooth muscle myosin in the molecular mechanism of muscle contraction. ..
  43. Alpert N, Brosseau C, Federico A, Krenz M, Robbins J, Warshaw D. Molecular mechanics of mouse cardiac myosin isoforms. Am J Physiol Heart Circ Physiol. 2002;283:H1446-54 pubmed
    ..These residues are localized to both the motor domain and the rod. These differences in sequence and mechanical performance may be an evolutionary attempt to match a myosin's mechanical behavior to the heart's power requirements. ..
  44. Comelekoglu U, Ozturk N. The stiffness and phase frequency response of taenia coli smooth muscle: comparison of the step and sinusoidal perturbation analysis. Med Eng Phys. 2008;30:380-6 pubmed
  45. Mazhari S, Selser C, Cremo C. Novel sensors of the regulatory switch on the regulatory light chain of smooth muscle Myosin. J Biol Chem. 2004;279:39905-14 pubmed
    ..This is the first demonstration of direct coupling of nucleotide binding to conformational changes in the N terminus of the regulatory light chain. ..
  46. Kashiwakura Y, Katoh Y, Tamayose K, Konishi H, Takaya N, Yuhara S, et al. Isolation of bone marrow stromal cell-derived smooth muscle cells by a human SM22alpha promoter: in vitro differentiation of putative smooth muscle progenitor cells of bone marrow. Circulation. 2003;107:2078-81 pubmed
    ..We demonstrated the presence of a cell population fated to become SMCs and followed their differentiation into SMCs among BMSCs. ..
  47. Lv Y, Lu S, Lu T, Kou J, Yu B. Homology model of nonmuscle myosin heavy chain IIA and binding mode analysis with its inhibitor blebbistatin. J Mol Model. 2013;19:1801-10 pubmed publisher
    ..These findings introduce new insights on the binding mode of blebbistatin and NMMHC IIA and novel leading compounds from natural products for NMMHC IIA-related diseases. ..
  48. Penheiter A, Bogoger M, Ellison P, Oswald B, Perkins W, Jones K, et al. H(2)O(2)-induced kinetic and chemical modifications of smooth muscle myosin: correlation to effects of H(2)O(2) on airway smooth muscle. J Biol Chem. 2007;282:4336-44 pubmed
    ..These data, when compared with the in vitro kinetic data, are consistent with a H(2)O(2)-induced loss of functional myosin heads in the muscle. ..
  49. Nelson W, Blakely S, Nesmelov Y, Thomas D. Site-directed spin labeling reveals a conformational switch in the phosphorylation domain of smooth muscle myosin. Proc Natl Acad Sci U S A. 2005;102:4000-5 pubmed
    ..These results support a model in which this disorder-to-order transition within the phosphorylation domain results in decreased head-head interactions, activating myosin in smooth muscle. ..
  50. Asuzu D, Hayashi Y, Izbeki F, Popko L, Young D, Bardsley M, et al. Generalized neuromuscular hypoplasia, reduced smooth muscle myosin and altered gut motility in the klotho model of premature aging. Neurogastroenterol Motil. 2011;23:e309-23 pubmed publisher
    ..As reduced fecal output in these mice occurs in the presence of accelerated colonic and whole-gut transit, it likely reflects reduced food intake rather than intestinal dysmotility. ..
  51. Colson B, Gruber S, Thomas D. Structural dynamics of muscle protein phosphorylation. J Muscle Res Cell Motil. 2012;33:419-29 pubmed publisher
    ..These studies, which offer atomic-resolution insight into the structural and functional dynamics of these phosphoproteins, were inspired in part by the ground-breaking work in this field by Michael and Kate Barany. ..
  52. Faragalla H, Marcon N, Yousef G, Streutker C. Immunohistochemical staining for smoothelin in the duplicated versus the true muscularis mucosae of Barrett esophagus. Am J Surg Pathol. 2011;35:55-9 pubmed publisher
    ..We hypothesized that in the esophagus, immunohistochemistry for smoothelin would differentiate the NMM from the TMM...
  53. Chang S, Hypolite J, Zderic S, Wein A, Chacko S, Disanto M. Increased corpus cavernosum smooth muscle tone associated with partial bladder outlet obstruction is mediated via Rho-kinase. Am J Physiol Regul Integr Comp Physiol. 2005;289:R1124-30 pubmed