minichromosome maintenance proteins


Summary: A family of proteins that were originally identified in SACCHAROMYCES CEREVISIAE as being essential for maintaining the structure of minichromosomes00. They form into a protein complex that has helicase activity and is involved in a variety of DNA-related functions including replication elongation, RNA transcription, chromatin remodeling, and genome stability.

Top Publications

  1. Gardner N, Gillespie P, Carrington J, Shanks E, McElroy S, Haagensen E, et al. The High-Affinity Interaction between ORC and DNA that Is Required for Replication Licensing Is Inhibited by 2-Arylquinolin-4-Amines. Cell Chem Biol. 2017;24:981-992.e4 pubmed publisher
    ..must be "licensed" for use in the upcoming S phase by being encircled by double hexamers of the minichromosome maintenance proteins MCM2-7...
  2. Ticau S, Friedman L, Champasa K, Corrêa I, Gelles J, Bell S. Mechanism and timing of Mcm2-7 ring closure during DNA replication origin licensing. Nat Struct Mol Biol. 2017;24:309-315 pubmed publisher
  3. Pérez Arnaiz P, Bruck I, Colbert M, Kaplan D. An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-? to Mcm2-7. Nucleic Acids Res. 2017;45:7261-7275 pubmed publisher
    ..We also detected diminished GINS and pol-? recruitment to the Mcm2-7 complex. We conclude that an intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly, and the recruitment of pol ? to Mcm2-7. ..
  4. Feng X, Matsuo K, Zhang T, Hu Y, Mays A, Browne J, et al. MicroRNA Profiling and Target Genes Related to Metastasis of Salivary Adenoid Cystic Carcinoma. Anticancer Res. 2017;37:3473-3481 pubmed
    ..05). miR-99a, miR-155, miR-130a, miR-342, and miR-205 may play a role in metastasis of SACC. MiR-155 may be involved in SACC metastasis through UBA2 pathways, and UBA2 may function as a biomarker/mediator of SACC metastasis. ..
  5. Izumi M, Mizuno T, Yanagi K, Sugimura K, Okumura K, Imamoto N, et al. The Mcm2-7-interacting domain of human mini-chromosome maintenance 10 (Mcm10) protein is important for stable chromatin association and origin firing. J Biol Chem. 2017;292:13008-13021 pubmed publisher
    ..These results suggest that human Mcm10 is bound to chromatin through the interaction with Mcm2-7 and is primarily involved in the initiation of DNA replication after loading of Cdc45 and Pol ?. ..
  6. Neves H, Kwok H. In sickness and in health: The many roles of the minichromosome maintenance proteins. Biochim Biophys Acta Rev Cancer. 2017;1868:295-308 pubmed publisher
    ..In this review, we aim to give an overview on the members of the MCM family, what their functions are in a healthy environment and how they are altered in cancer. ..
  7. Walters A, Chong J. Non-essential MCM-related proteins mediate a response to DNA damage in the archaeon Methanococcus maripaludis. Microbiology. 2017;163:745-753 pubmed publisher
    ..M. maripaludis S2 is the first archaeon in which MCM proteins have been shown to influence the DNA damage response. ..
  8. Natsume T, Nishimura K, Minocherhomji S, Bhowmick R, Hickson I, Kanemaki M. Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. 2017;31:816-829 pubmed publisher
    ..We propose that stalled replication forks can be restarted in S phase via homologous recombination using MCM8-9 as an alternative replicative helicase. ..
  9. He D, Ren B, Liu S, Tan L, Cieply K, Tseng G, et al. Oncogenic activity of amplified miniature chromosome maintenance 8 in human malignancies. Oncogene. 2017;36:3629-3639 pubmed publisher
    ..The cyclin D1/MCM8 interaction is required for Rb phosphorylation and S-phase entry in cancer cells. As a result, our study showed that copy number increase and overexpression of MCM8 may play critical roles in human cancer development. ..

More Information


  1. Senfter D, Erkan E, Ozer E, Jungwirth G, Madlener S, Kool M, et al. Overexpression of minichromosome maintenance protein 10 in medulloblastoma and its clinical implications. Pediatr Blood Cancer. 2017;64: pubmed publisher
    ..Taken together, our study reveals that the pre-RC is dysregulated in MB. In addition, MCM10, a member of this complex, is significantly overexpressed in MB and is required for tumor cell proliferation. ..
  2. Riera A, Barbon M, Noguchi Y, Reuter L, Schneider S, Speck C. From structure to mechanism-understanding initiation of DNA replication. Genes Dev. 2017;31:1073-1088 pubmed publisher
  3. SIMON N, Bochman M, Seguin S, Brodsky J, Seibel W, Schwacha A. Ciprofloxacin is an inhibitor of the Mcm2-7 replicative helicase. Biosci Rep. 2013;33: pubmed publisher
    ..Our results indicate that ciprofloxacin targets Mcm2-7 in vitro, and support the feasibility of developing specific quinolone-based inhibitors of Mcm2-7 for therapeutic and experimental applications. ..
  4. Ganaie S, Zou W, Xu P, Deng X, Kleiboeker S, Qiu J. Phosphorylated STAT5 directly facilitates parvovirus B19 DNA replication in human erythroid progenitors through interaction with the MCM complex. PLoS Pathog. 2017;13:e1006370 pubmed publisher
    ..Our results demonstrated that pimozide could be a promising antiviral drug for treatment of B19V-related diseases. ..
  5. Hizume K, Kominami H, Kobayashi K, Yamada H, Araki H. Flexible DNA Path in the MCM Double Hexamer Loaded on DNA. Biochemistry. 2017;56:2435-2445 pubmed publisher
  6. Alasiri S, Basit S, Wood Trageser M, Yatsenko S, Jeffries E, Surti U, et al. Exome sequencing reveals MCM8 mutation underlies ovarian failure and chromosomal instability. J Clin Invest. 2015;125:258-62 pubmed publisher
    ..P149R to sites of DNA damage. Our study identifies an autosomal recessive disorder caused by an MCM8 mutation that manifests with endocrine dysfunction and genomic instability. ..
  7. Inano S, Sato K, Katsuki Y, Kobayashi W, Tanaka H, Nakajima K, et al. RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol Cell. 2017;66:622-634.e8 pubmed publisher
    ..Collectively, our data reveal a mechanism that facilitates timely removal of RPA and RAD51 from DNA damage sites, which is crucial for progression to the late-phase HR and suppression of the FA phenotype. ..
  8. Bouali N, Francou B, Bouligand J, Imanci D, Dimassi S, Tosca L, et al. New MCM8 mutation associated with premature ovarian insufficiency and chromosomal instability in a highly consanguineous Tunisian family. Fertil Steril. 2017;108:694-702 pubmed publisher
    ..Our findings provide additional support to the view that MCM8 mutations are involved in the primary ovarian insufficiency phenotype. ..
  9. Zhai Y, Li N, Jiang H, Huang X, Gao N, Tye B. Unique Roles of the Non-identical MCM Subunits in DNA Replication Licensing. Mol Cell. 2017;67:168-179 pubmed publisher
  10. Coster G, Diffley J. Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading. Science. 2017;357:314-318 pubmed publisher
    ..We propose that quasi-symmetrical loading of individual MCM hexamers by ORC and directed MCM translocation into double hexamers acts as a unifying mechanism for the establishment of bidirectional replication in archaea and eukaryotes. ..
  11. Bell S. DNA Replication. Terminating the replisome. Science. 2014;346:418-9 pubmed publisher