phosphate binding proteins


Summary: Proteins that bind to and are involved in the metabolism of phosphate ions.

Top Publications

  1. Rallu F, Gruss A, Ehrlich S, Maguin E. Acid- and multistress-resistant mutants of Lactococcus lactis : identification of intracellular stress signals. Mol Microbiol. 2000;35:517-28 pubmed
    ..Our results provide a link between the physiological state of the cell and the level of stress tolerance and establish a role for the stringent response in acid stress response regulation...
  2. Runyen Janecky L, Payne S. Identification of chromosomal Shigella flexneri genes induced by the eukaryotic intracellular environment. Infect Immun. 2002;70:4379-88 pubmed
    ..Both mutants were as effective as the parent strain in inducing apoptosis in a macrophage cell line. ..
  3. Peirs P, Lefevre P, Boarbi S, Wang X, Denis O, Braibant M, et al. Mycobacterium tuberculosis with disruption in genes encoding the phosphate binding proteins PstS1 and PstS2 is deficient in phosphate uptake and demonstrates reduced in vivo virulence. Infect Immun. 2005;73:1898-902 pubmed
    ..the pstS1 and pstS2 genes genetically inactivated, we showed that these pstS genes encode high-affinity phosphate binding proteins. In a mouse infection model, both mutants were attenuated in virulence, suggesting that M...
  4. D az M, Esteban A, Fern ndez Abalos J, Santamar a R. The high-affinity phosphate-binding protein PstS is accumulated under high fructose concentrations and mutation of the corresponding gene affects differentiation in Streptomyces lividans. Microbiology. 2005;151:2583-92 pubmed publisher
    ..The present work demonstrates that carbon and phosphate metabolism are linked in the regulation of genes and that this can trigger the genetic switch towards morphogenesis...
  5. Raghothama K. Phosphate transport and signaling. Curr Opin Plant Biol. 2000;3:182-7 pubmed
    ..Recent developments in plant genomics will soon allow the complete dissection of the signal transduction pathway(s) associated with plant responses to Pi limitation in the rhizosphere. ..
  6. Garg J, Chasan Taber S, Blair A, Plone M, Bommer J, Raggi P, et al. Effects of sevelamer and calcium-based phosphate binders on uric acid concentrations in patients undergoing hemodialysis: a randomized clinical trial. Arthritis Rheum. 2005;52:290-5 pubmed
    ..02). In a randomized clinical trial comparing sevelamer and calcium-based phosphate binders, treatment with sevelamer was associated with a significant reduction in serum uric acid concentrations. ..
  7. Smith D, Girodat D, Wieden H, Selinger L. Streamlined purification of fluorescently labeled Escherichia coli phosphate-binding protein (PhoS) suitable for rapid-kinetics applications. Anal Biochem. 2017;537:106-113 pubmed publisher
  8. Leggewie G, Willmitzer L, Riesmeier J. Two cDNAs from potato are able to complement a phosphate uptake-deficient yeast mutant: identification of phosphate transporters from higher plants. Plant Cell. 1997;9:381-92 pubmed publisher
    ..No expression was found under optimized nutrition conditions or when other macronutrients were lacking...
  9. Mitsukawa N, Okumura S, Shirano Y, Sato S, Kato T, Harashima S, et al. Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions. Proc Natl Acad Sci U S A. 1997;94:7098-102 pubmed
    ..The transgenic cells exhibited increased biomass production when the supply of phosphate was limited, establishing gene engineering of phosphate transport as one approach toward enhancing plant cell growth. ..

More Information

Publications106 found, 100 shown here

  1. Yuan Z, Zaheer R, Finan T. Regulation and properties of PstSCAB, a high-affinity, high-velocity phosphate transport system of Sinorhizobium meliloti. J Bacteriol. 2006;188:1089-102 pubmed
    ..Thus, metabolism of alfalfa nodule bacteroids is not Pi limited. ..
  2. Williams K, Barycka K, Zella J, DeLuca H. Diaminobutane (DAB) dendrimers are potent binders of oral phosphate. J Bone Miner Res. 2009;24:97-101 pubmed publisher
    ..These data suggest that dendrimer compounds are of great potential use in the binding of food phosphate for the management of hyperparathyroidism secondary to chronic kidney disease. ..
  3. Esteban A, Diaz M, Yepes A, Santamaría R. Expression of the pstS gene of Streptomyces lividans is regulated by the carbon source and is partially independent of the PhoP regulator. BMC Microbiol. 2008;8:201 pubmed publisher
    ..2.- The stretch of eight degenerated repeats present in the pstS promoter may act as a binding site for a repressor. 3.- There is a basal expression of the pstS gene that is not controlled by the main regulator: PhoP. ..
  4. Renault F, Carus T, Cléry Barraud C, Elias M, Chabriere E, Masson P, et al. Integrative analytical approach by capillary electrophoresis and kinetics under high pressure optimized for deciphering intrinsic and extrinsic cofactors that modulate activity and stability of human paraoxonase (PON1). J Chromatogr B Analyt Technol Biomed Life Sci. 2010;878:1346-55 pubmed publisher
    ..Relevance of these data to understand in vivo physiological PON1 functioning is mandatory. ..
  5. Farmaki T. Use of a Phosphatidylinositol Phosphate Affinity Chromatography (PIP Chromatography) for the Isolation of Proteins Involved in Protein Quality Control and Proteostasis Mechanisms in Plants. Methods Mol Biol. 2016;1450:223-32 pubmed publisher
    ..protocol based on an affinity chromatographic approach aiming at the isolation of phosphatidyl inositol phosphate binding proteins, a procedure which results into the enrichment and purification of several members of the proteostasis ..
  6. Yang Y, Ballent W, Mayer B. High-affinity phosphate-binding protein (PBP) for phosphorous recovery: proof of concept using recombinant Escherichia coli. FEMS Microbiol Lett. 2016;363: pubmed
    ..1% P recovery within 3 h. This study provides proof of concept of the feasibility of using PBP to recover P. ..
  7. Helps C, McGivan J. Adaptive regulation of Na(+)-dependent phosphate transport in the bovine renal epithelial cell line NBL-1. Identification of the phosphate transporter as a 55-kDa glycoprotein. Eur J Biochem. 1991;200:797-803 pubmed
    ..The symport of phosphate and Na+ is electroneutral with a stoichiometry of 2Na+/phosphate at physiological pH values. ..
  8. Coladonato J. Control of hyperphosphatemia among patients with ESRD. J Am Soc Nephrol. 2005;16 Suppl 2:S107-14 pubmed
    ..Concerns of long-term administration and toxicity exist. Furthermore, these agents are significantly more expensive than calcium salts, which may contribute to patient noncompliance. ..
  9. Genchi G, Petrone G, De Palma A, Cambria A, Palmieri F. Interaction of phenylisothiocyanates with the mitochondrial phosphate carrier. I. Covalent modification and inhibition of phosphate transport. Biochim Biophys Acta. 1988;936:413-20 pubmed
    ..Lysine(s) of the former type are located at the surface of the membrane and are topologically related to the functional SH groups of the protein. Lysine residue(s) of the latter type are buried in the hydrophobic phase of the membrane. ..
  10. Daram P, Brunner S, Rausch C, Steiner C, Amrhein N, Bucher M. Pht2;1 encodes a low-affinity phosphate transporter from Arabidopsis. Plant Cell. 1999;11:2153-66 pubmed
    ..Pht2;1 thus differs from members of the recently described plant Pi transporter family in primary structure, affinity for Pi, and presumed function. ..
  11. Gu H, Lalonde S, Okumoto S, Looger L, Scharff Poulsen A, Grossman A, et al. A novel analytical method for in vivo phosphate tracking. FEBS Lett. 2006;580:5885-93 pubmed
    ..FLIPPi sensors are suitable for real-time monitoring of P(i) metabolism in living cells, providing a new tool for fluxomics, analysis of pathophysiology or changes of P(i) during cell migration. ..
  12. Rochu D, Chabriere E, Renault F, Elias M, Cléry Barraud C, Masson P. Stabilization of the active form(s) of human paraoxonase by human phosphate-binding protein. Biochem Soc Trans. 2007;35:1616-20 pubmed
    ..Maintaining a stable environment for such administrable human rPON1 should, at least, preserve the anti-atherogenic activity of the enzyme. ..
  13. Wisniewski E, Kunz W, Gellerich F. Phosphate affects the distribution of flux control among the enzymes of oxidative phosphorylation in rat skeletal muscle mitochondria. J Biol Chem. 1993;268:9343-6 pubmed
    ..This indicates that under the conditions of intermediate state respiration, all of the other reactions have a negligible controlling influence on oxidative phosphorylation in skeletal muscle mitochondria. ..
  14. Zara V, Dietmeier K, Palmisano A, Vozza A, Rassow J, Palmieri F, et al. Yeast mitochondria lacking the phosphate carrier/p32 are blocked in phosphate transport but can import preproteins after regeneration of a membrane potential. Mol Cell Biol. 1996;16:6524-31 pubmed
  15. Wang Z, Luecke H, Yao N, Quiocho F. A low energy short hydrogen bond in very high resolution structures of protein receptor--phosphate complexes. Nat Struct Biol. 1997;4:519-22 pubmed
  16. Hoffer S, Tommassen J. The phosphate-binding protein of Escherichia coli is not essential for P(i)-regulated expression of the pho regulon. J Bacteriol. 2001;183:5768-71 pubmed
    ..We demonstrate that P(i)-controlled expression is restored when the activity of the P(i) transporter PitA or PitB is increased. Apparently, PstS is not an essential component of the signal transduction pathway...
  17. Siehnel R, Worobec E, Hancock R. Regulation of components of the Pseudomonas aeruginosa phosphate-starvation-inducible regulon in Escherichia coli. Mol Microbiol. 1988;2:347-52 pubmed
    ..Since P. aeruginosa and E. coli belong to different families and have quite different GC contents, these data suggest strong evolutionary conservation of regulatory elements of the Pho regulon. ..
  18. Engstová H, Zackova M, Ruzicka M, Meinhardt A, Hanus J, Kramer R, et al. Natural and azido fatty acids inhibit phosphate transport and activate fatty acid anion uniport mediated by the mitochondrial phosphate carrier. J Biol Chem. 2001;276:4683-91 pubmed
    ..Hence, the demonstrated PIC interaction with monovalent long-chain FA anions, but with divalent phosphonates of short chain only, indicates a pattern distinct from that valid for the mitochondrial uncoupling protein-1. ..
  19. Morales R, Berna A, Carpentier P, Contreras Martel C, Renault F, Nicodeme M, et al. Serendipitous discovery and X-ray structure of a human phosphate binding apolipoprotein. Structure. 2006;14:601-9 pubmed
    ..HPBP is the only known transporter capable of binding phosphate ions in human plasma and may become a new predictor of or a potential therapeutic agent for phosphate-related diseases such as atherosclerosis. ..
  20. Luecke H, Quiocho F. High specificity of a phosphate transport protein determined by hydrogen bonds. Nature. 1990;347:402-6 pubmed publisher
    ..These features are fundamental to understanding phosphate transport systems and molecular recognition of charged substrates or ions in other biological processes...
  21. Karamanidou C, Clatworthy J, Weinman J, Horne R. A systematic review of the prevalence and determinants of nonadherence to phosphate binding medication in patients with end-stage renal disease. BMC Nephrol. 2008;9:2 pubmed publisher
    ..There is a need for further, high-quality research to explore these factors in more detail, with the aim of informing the design of an intervention to facilitate adherence. ..
  22. Gandhi D, Chanalia P, Attri P, Dhanda S. Dipeptidyl peptidase-II from probiotic Pediococcus acidilactici: Purification and functional characterization. Int J Biol Macromol. 2016;93:919-932 pubmed publisher
    ..fragments obtained by MALDI-TOF revealed an evolutionary relationship between DPP-II of prokaryotes and phosphate binding proteins. Secondary and three-dimensional structure of enzyme was also deduced by in-silico approach...
  23. Hoffer S, Schoondermark P, van Veen H, Tommassen J. Activation by gene amplification of pitB, encoding a third phosphate transporter of Escherichia coli K-12. J Bacteriol. 2001;183:4659-63 pubmed
    ..A revertant of a pitA pstS double mutant that could grow on P(i) was isolated. We demonstrate that the expression of a new P(i) transporter, PitB, is activated in this strain by a gene amplification event...
  24. Humphreys D, Heywood S, King L, Bowering L, Turner J, Lane S. Engineering of Escherichia coli to improve the purification of periplasmic Fab' fragments: changing the pI of the chromosomally encoded PhoS/PstS protein. Protein Expr Purif. 2004;37:109-18 pubmed
    ..This exemplifies the strategy of the modification of host proteins with the aim of simplifying the production of heterologous proteins. ..
  25. Qunibi W. Effects of sevelamer and calcium on coronary artery calcification in patients new to dialysis: time for better controlled clinical trials. Kidney Int. 2006;69:1093 pubmed
  26. Almirall J, Daly J, Valenzuela M, Mujica M. The safety of phosphate binders. Expert Opin Drug Saf. 2006;5:675-86 pubmed
    ..Also, factors related with cost, tolerance, palatability, safety and efficacy are important. In this article, the authors review the advantages and drawbacks of conventional and emerging therapies in phosphorous binding. ..
  27. Okoh M, Hunter J, Corrie J, Webb M. A biosensor for inorganic phosphate using a rhodamine-labeled phosphate binding protein. Biochemistry. 2006;45:14764-71 pubmed
    ..Advantages of the new sensor are discussed, both in terms of the use of a rhodamine fluorophore and the potential of this double labeling strategy. ..
  28. Pegos V, Hey L, LaMirande J, Pfeffer R, Lipsh R, Amitay M, et al. Phosphate-binding protein from Polaromonas JS666: purification, characterization, crystallization and sulfur SAD phasing. Acta Crystallogr F Struct Biol Commun. 2017;73:342-346 pubmed publisher
    ..Here, the expression, purification, characterization, crystallization and X-ray diffraction data collection to 1.35?Å resolution of the PBP from Polaromonas JS666 are reported...
  29. Phelps A, Briggs C, Haefele A, Mincone L, Ligeti E, Wohlrab H. Mitochondrial phosphate transport protein. Reversions of inhibitory conservative mutations identify four helices and a nonhelix protein segment with transmembrane interactions and Asp39, Glu137, and Ser158 as nonessential for transport. Biochemistry. 2001;40:2080-6 pubmed
    ..These dramatic conformational differences also suggest an interaction between TM helices D and E. The results are discussed in terms of TM helix movements and changes in the PTP monomer/dimer ratio. ..
  30. Suki W. Effects of sevelamer and calcium-based phosphate binders on mortality in hemodialysis patients: results of a randomized clinical trial. J Ren Nutr. 2008;18:91-8 pubmed
    ..06) and fewer hospital days (P = .09). A statistically significant reduction in mortality in the overall study population was not observed. Sevelamer was associated with a survival benefit among patients > or = 65 years of age. ..
  31. Phelps A, Schobert C, Wohlrab H. Cloning and characterization of the mitochondrial phosphate transport protein gene from the yeast Saccharomyces cerevisiae. Biochemistry. 1991;30:248-52 pubmed
    ..Mersalyl sensitivity has been retained and must be due to one of its three cysteines. Among these three cysteines, only Cys-28, located in the first hydrophobic segment, is conserved between the yeast and the beef protein. ..
  32. Bun Ya M, Nishimura M, Harashima S, Oshima Y. The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter. Mol Cell Biol. 1991;11:3229-38 pubmed
    ..Gene disruption of PHO84 was not lethal. By comparison of nucleotide sequences and by tetrad analysis with GAL80 as a standard, the PHO84 locus was mapped at a site beside the TUB3 locus on the left arm of chromosome XIII. ..
  33. Kim S, Makino K, Amemura M, Nakata A, Shinagawa H. Mutational analysis of the role of the first helix of region 4.2 of the sigma 70 subunit of Escherichia coli RNA polymerase in transcriptional activation by activator protein PhoB. Mol Gen Genet. 1995;248:1-8 pubmed
    ..These results provide further evidence that the first helix plays an important role in the specific interaction between RNA polymerase and PhoB protein bound to the pho promoters in transcriptional activation. ..
  34. Wang Z, Choudhary A, Ledvina P, Quiocho F. Fine tuning the specificity of the periplasmic phosphate transport receptor. Site-directed mutagenesis, ligand binding, and crystallographic studies. J Biol Chem. 1994;269:25091-4 pubmed
  35. Zackova M, Kramer R, Jezek P. Interaction of mitochondrial phosphate carrier with fatty acids and hydrophobic phosphate analogs. Int J Biochem Cell Biol. 2000;32:499-508 pubmed
    ..This domain allows for FA anion uniport via the phosphate carrier and consequently, FA cycling that should lead to uncoupling in mitochondria. This might be considered as a side function of this carrier. ..
  36. Morita T, Amemura M, Makino K, Shinagawa H, Magota K, Otsuji N, et al. Hyperproduction of phosphate-binding protein, phoS, and pre-phoS proteins in Escherichia coli carrying a cloned phoS gene. Eur J Biochem. 1983;130:427-35 pubmed
    ..Kinetics and regulation of accumulation of these proteins were studied. This system will be useful for preparation and purification of the precursor protein for biochemical studies in relation to the mechanism of protein secretion. ..
  37. Jabs E, Thomas P, Bernstein M, Coss C, Ferreira G, Pedersen P. Chromosomal localization of genes required for the terminal steps of oxidative metabolism: alpha and gamma subunits of ATP synthase and the phosphate carrier. Hum Genet. 1994;93:600-2 pubmed
    ..Human homologues of the alpha subunit are on chromosomes 9 and 18, the gamma subunit are on chromosomes 10 and 14, and the PHC was localized to chromosome 12. ..
  38. Marsh S, Carter N, Dolce V, Iacobazzi V, Palmieri F. Chromosomal localization of the mitochondrial phosphate carrier gene PHC to 12q23. Genomics. 1995;29:814-5 pubmed
  39. Tanabe M, Mirza O, Bertrand T, Atkins H, Titball R, Iwata S, et al. Structures of OppA and PstS from Yersinia pestis indicate variability of interactions with transmembrane domains. Acta Crystallogr D Biol Crystallogr. 2007;63:1185-93 pubmed
  40. Miller D, Miller A. A family of retroviruses that utilize related phosphate transporters for cell entry. J Virol. 1994;68:8270-6 pubmed
  41. Pratt R, Ferreira G, Pedersen P. Mitochondrial phosphate transport. Import of the H+/Pi symporter and role of the presequence. J Biol Chem. 1991;266:1276-80 pubmed
  42. Lee T, Makino K, Shinagawa H, Nakata A. Overproduction of acetate kinase activates the phosphate regulon in the absence of the phoR and phoM functions in Escherichia coli. J Bacteriol. 1990;172:2245-9 pubmed
    ..Overexpression of ackA elevated the intracellular ATP concentration, an effect that might be related to activation of the phosphate regulon in the phoR phoM strain. ..
  43. Lee T, Makino K, Shinagawa H, Amemura M, Nakata A. Phosphate regulon in members of the family Enterobacteriaceae: comparison of the phoB-phoR operons of Escherichia coli, Shigella dysenteriae, and Klebsiella pneumoniae. J Bacteriol. 1989;171:6593-9 pubmed
    ..Nucleotide sequences of phoB and phoR of S. dysenteriae Sh and K. pneumoniae are highly homologous to those of E. coli, except for a single base insertion found in phoR of S. dysenteriae Sh...
  44. Surin B, Jans D, Fimmel A, Shaw D, Cox G, Rosenberg H. Structural gene for the phosphate-repressible phosphate-binding protein of Escherichia coli has its own promoter: complete nucleotide sequence of the phoS gene. J Bacteriol. 1984;157:772-8 pubmed
    ..However, an alternative promoter region has been identified on the basis of homology with the promoter regions of the phoA and phoE genes, the structural genes for alkaline phosphatase and outer-membrane pore protein e, respectively. ..
  45. Magota K, Otsuji N, Miki T, Horiuchi T, Tsunasawa S, Kondo J, et al. Nucleotide sequence of the phoS gene, the structural gene for the phosphate-binding protein of Escherichia coli. J Bacteriol. 1984;157:909-17 pubmed
    ..the nucleotide sequence of the regulatory region of phoS was compared with those of phoA and phoE, the genes constituting the pho regulon. ..
  46. Hopkins C, White F, Heaton L, Guikema J, Leach J. A homolog of an Escherichia coli phosphate-binding protein gene from Xanthomonas oryzae pv. oryzae. DNA Seq. 1995;5:299-305 pubmed
    ..coli. Amino terminal sequencing revealed that a signal peptide is removed during transport to the periplasm in E. coli. ..
  47. Kozak S, Siess D, Kavanaugh M, Miller A, Kabat D. The envelope glycoprotein of an amphotropic murine retrovirus binds specifically to the cellular receptor/phosphate transporter of susceptible species. J Virol. 1995;69:3433-40 pubmed
    ..We conclude that amphotropic gp70 binds more strongly to rRam-1 than to the homologous hamster protein and that this stable attachment is necessary for infection, interference, membrane fusion, and pathogenesis. ..
  48. Scanlan D, Silman N, Donald K, Wilson W, Carr N, Joint I, et al. An immunological approach to detect phosphate stress in populations and single cells of photosynthetic picoplankton. Appl Environ Microbiol. 1997;63:2411-20 pubmed
    ..strain WH7803 protein, including those residues in Escherichia coli PstS known to be directly involved in phosphate binding. ..
  49. Kramer R. Mitochondrial carrier proteins can reversibly change their transport mode: the cases of the aspartate/glutamate and the phosphate carrier. Exp Physiol. 1998;83:259-65 pubmed
    ..Under these conditions, a fourth transport mode of the phosphate carrier was observed, namely an action as anion-selective channel, which could be reversibly blocked by phosphate. ..
  50. Duwat P, Ehrlich S, Gruss A. Effects of metabolic flux on stress response pathways in Lactococcus lactis. Mol Microbiol. 1999;31:845-58 pubmed
    ..Our results reveal that cellular metabolic pathways are intimately related to stress response and that the flux of particular metabolites, notably guanine and phosphate, may be implicated in stress response in lactococci...
  51. Neuhaus H, Wagner R. Solute pores, ion channels, and metabolite transporters in the outer and inner envelope membranes of higher plant plastids. Biochim Biophys Acta. 2000;1465:307-23 pubmed
    ..The plastidic ATP/ADP transporter also contains 12 putative transmembrane domains and possesses striking structural similarity to ATP/ADP transporters found in intracellular, human pathogenic bacteria. ..
  52. Chiou T, Liu H, Harrison M. The spatial expression patterns of a phosphate transporter (MtPT1) from Medicago truncatula indicate a role in phosphate transport at the root/soil interface. Plant J. 2001;25:281-93 pubmed
    ..truncatula roots. MtPT1 shows expression patterns consistent with a role specifically in the acquisition of phosphate from the soil and is distinct from the other phosphate transporter of this class described to date. ..
  53. Ostap E, Lin T, Rosenfeld S, Tang N. Mechanism of regulation of Acanthamoeba myosin-IC by heavy-chain phosphorylation. Biochemistry. 2002;41:12450-6 pubmed
    ..We propose that the TEDS-containing actin-binding loop plays a direct role in regulating phosphate release and the force-generating (A-to-R) transition of myosin-IC. ..
  54. Webb M. A tale of the unexpected. Structure. 2006;14:391-2 pubmed
  55. Garside R, Pitt M, Anderson R, Mealing S, D Souza R, Stein K. The cost-utility of cinacalcet in addition to standard care compared to standard care alone for secondary hyperparathyroidism in end-stage renal disease: a UK perspective. Nephrol Dial Transplant. 2007;22:1428-36 pubmed
    ..Unless the cost of cinacalcet is considerably reduced, it is unlikely to be considered a cost-effective treatment for people with SHPT. ..
  56. Peliska J, O Leary M. Sulfuryl transfer catalyzed by phosphokinases. Biochemistry. 1991;30:1049-57 pubmed
    ..These results suggest that the role of the metal ion in nucleoside diphosphate kinase is to coordinate the alpha, beta-phosphates of the substrate. Sulfuryl and phosphoryl transfer probably occur through dissociative transition states. ..
  57. Levitz R, Klar A, Sar N, Yagil E. A new locus in the phosphate specific transport (PST) region of Escherichia coli. Mol Gen Genet. 1984;197:98-103 pubmed
    ..The genetic order obtained was phoS64-phoU35-pstB401-phoT-phoS-ilvC. The data indicate that phoS64 belongs to a different complementation unit in the PST region not known hitherto. We propose to name it phoV. ..
  58. Hiratsu K, Nakata A, Shinagawa H, Makino K. Autophosphorylation and activation of transcriptional activator PhoB of Escherichia coli by acetyl phosphate in vitro. Gene. 1995;161:7-10 pubmed
    ..These results indicate that PhoB is an enzyme that catalyzes its own phosphorylation using acP, a low-molecular-weight metabolic intermediate. ..
  59. Scholten M, Janssen R, Bogaarts C, van Strien J, Tommassen J. The pho regulon of Shigella flexneri. Mol Microbiol. 1995;15:247-54 pubmed
    ..coli PhoB were detected. Site-directed mutagenesis revealed that one of these deviations, i.e. Leu-172, which is Arg in E. coli PhoB, is responsible for the lack of expression of the PhoE protein in S. flexneri...
  60. van Veen H, Abee T, Kortstee G, Konings W, Zehnder A. Translocation of metal phosphate via the phosphate inorganic transport system of Escherichia coli. Biochemistry. 1994;33:1766-70 pubmed
    ..These results have been evaluated in terms of ordered binding and dissociation of metal phosphate and proton on the outer and inner surface of the cytoplasmic membrane. ..
  61. Dolce V, Iacobazzi V, Palmieri F, Walker J. The sequences of human and bovine genes of the phosphate carrier from mitochondria contain evidence of alternatively spliced forms. J Biol Chem. 1994;269:10451-60 pubmed
    ..As the function of this region of the phosphate carrier is not known, the effects of the changes on carrier function are not understood at present. ..
  62. Booth J, Guidotti G. Phosphate transport in yeast vacuoles. J Biol Chem. 1997;272:20408-13 pubmed
    ..Transport is strongly pH-dependent with increasing activity at lower pH. Similar phosphate transport behavior was observed in right-side-out vacuolar membrane vesicles. ..
  63. Liu H, Trieu A, Blaylock L, Harrison M. Cloning and characterization of two phosphate transporters from Medicago truncatula roots: regulation in response to phosphate and to colonization by arbuscular mycorrhizal (AM) fungi. Mol Plant Microbe Interact. 1998;11:14-22 pubmed publisher
  64. Fristedt U, van der Rest M, Poolman B, Konings W, Persson B. Studies of cytochrome c oxidase-driven H(+)-coupled phosphate transport catalyzed by the Saccharomyces cerevisiae Pho84 permease in coreconstituted vesicles. Biochemistry. 1999;38:16010-5 pubmed
    ..The metal dependency of transport indicates that a metal-phosphate complex is the translocated substrate. ..
  65. Rankin C, Wicks S. Mutations of the caenorhabditis elegans brain-specific inorganic phosphate transporter eat-4 affect habituation of the tap-withdrawal response without affecting the response itself. J Neurosci. 2000;20:4337-44 pubmed
    ..Our results suggest that neurotransmitter regulation plays a role in habituation and may play a role in dishabituation. ..
  66. Hernando N, Forster I, Biber J, Murer H. Molecular characteristics of phosphate transporters and their regulation. Exp Nephrol. 2000;8:366-75 pubmed
    ..The intrinsic electrogenicity of NaPi-IIa, has allowed detailed studies of the transport kinetics of NaPi-IIa and, combined with mutagenesis methods, structure-function information at the protein level is emerging. ..
  67. Granjon T, Vacheron M, Vial C, Buchet R. Structural changes of mitochondrial creatine kinase upon binding of ADP, ATP, or Pi, observed by reaction-induced infrared difference spectra. Biochemistry. 2001;40:2988-94 pubmed
  68. Rodriguez A, Troye Blomberg M, Lindroth K, Ivanyi J, Singh M, Fernandez C. B- and T-cell responses to the mycobacterium surface antigen PstS-1 in the respiratory tract and adjacent tissues. Role of adjuvants and routes of immunization. Vaccine. 2003;21:458-67 pubmed
    ..Thus, i.n. route of immunization favors the induction of mucosal and systemic immune responses, while the Th cell development at mucosal inductive site is influenced by the adjuvant used for immunizations. ..
  69. Aguena M, Spira B. RT-PCR of long prokaryotic operon transcripts without DNase treatment. J Microbiol Methods. 2003;55:419-23 pubmed
    ..To avoid amplification of genomic DNA, the hybrid-primer molecules that were not incorporated into the first-strand cDNA were removed by RNase H treatment followed by ultrafiltration...
  70. Dyhrman S, Haley S. Phosphorus scavenging in the unicellular marine diazotroph Crocosphaera watsonii. Appl Environ Microbiol. 2006;72:1452-8 pubmed
    ..watsonii has a robust capacity for scavenging phosphorus in oligotrophic systems, although this capacity differs from that of other marine cyanobacterial genera, such as Synechococcus, Prochlorococcus, and Trichodesmium. ..
  71. Yanuar A, Sakurai S, Kitano K, Hakoshima T. Crystal structure of human Rad GTPase of the RGK-family. Genes Cells. 2006;11:961-8 pubmed
    ..This similarity seems to be endowed by the conserved hydrogen bonding interactions with the guanine base-recognition loops and the magnesium ion that has a typical octahedral coordination shell identical to that in H-Ras. ..
  72. Li B, Turuvekere S, Agrawal M, La D, Ramani K, Kihara D. Characterization of local geometry of protein surfaces with the visibility criterion. Proteins. 2008;71:670-83 pubmed
    ..Because of its intuitiveness and simplicity, the visibility criterion will lay the foundation for characterization and function annotation of local shape of proteins. ..
  73. Liebschner D, Elias M, Moniot S, Fournier B, Scott K, Jelsch C, et al. Elucidation of the phosphate binding mode of DING proteins revealed by subangstrom X-ray crystallography. J Am Chem Soc. 2009;131:7879-86 pubmed publisher
    ..PfluDING structures reveal that only dibasic phosphate binds to the protein at both acidic and basic phosphate, suggesting that the protein binding site environment stabilizes the HPO(4)(2-) form of phosphate. ..
  74. Ligeti E, Fonyo A. Phosphate transport protein of rat heart mitochondria: location of its SH-groups and exploration of their environment. Biochim Biophys Acta. 1989;973:170-5 pubmed
    ..It is suggested that the amino-acid sequence around the NEM-reactive cysteine (i.e., Lys-41 - Cys-42 - Arg-43) does not participate in substrate binding. ..
  75. Braibant M, Lefevre P, De Wit L, Ooms J, Peirs P, Huygen K, et al. Identification of a second Mycobacterium tuberculosis gene cluster encoding proteins of an ABC phosphate transporter. FEBS Lett. 1996;394:206-12 pubmed
    ..bovis BCG culture filtrate antigen (immunodominant in H-2b haplotype type mice). Finally, a signature motif identifying integral transmembrane proteins of prokaryotic phosphate binding-dependent permeases is proposed...
  76. Thangaraj H, Bull T, De Smet K, Hill M, Rouse D, Moreno C, et al. Duplication of genes encoding the immunodominant 38 kDa antigen in Mycobacterium intracellulare. FEMS Microbiol Lett. 1996;144:235-40 pubmed
    ..We also identified and characterised other pst genes that may constitute an operon. Considering that multiple isoforms of PstS are present in mycobacteria the possible role of pstS1 genes for pathogenesis is discussed. ..
  77. Muchhal U, Pardo J, Raghothama K. Phosphate transporters from the higher plant Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1996;93:10519-23 pubmed
    ..thaliana. The transcripts of both genes are expressed in roots and are not detectable in leaves. The steady-state level of their mRNAs increases in response to phosphate starvation. ..
  78. Spira B, Yagil E. The relation between ppGpp and the PHO regulon in Escherichia coli. Mol Gen Genet. 1998;257:469-77 pubmed
    ..We propose that the presence of ppGpp in the cell, but not its accumulation as a result of the starvation stress, is important for the expression of the PHO genes. ..
  79. Huang C, Barker S, Langridge P, Smith F, Graham R. Zinc deficiency up-regulates expression of high-affinity phosphate transporter genes in both phosphate-sufficient and -deficient barley roots. Plant Physiol. 2000;124:415-22 pubmed
    ..The significance of Zn involvement in the regulation of genes involved in P uptake is discussed. ..
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    ..This mutation greatly decreased the amount of PitA protein present in cell membranes, indicating that the aspartic acid substitution disrupts protein structure. ..
  82. Webb M. Development of fluorescent biosensors for probing the function of motor proteins. Mol Biosyst. 2007;3:249-56 pubmed
    ..The interplay between the possibilities for design and understanding the mechanism of the signal are discussed. Examples are described of how these sensors have been applied to understanding myosin and helicase motors. ..
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    ..A hybrid recombinant PON1 was shown more thermostable than the human enzyme, and its stability was unaffected by the presence of HPBP. Altogether, the results strongly encourage further study of the human enzyme. ..
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    ..The recent development of low phosphorus-containing food products represents a very useful addition for CKD patients...
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    ..Early detection of elevated PTH levels with appropriate intervention using active vitamin D therapy, even in the absence of elevated serum phosphorus and reduced serum calcium, is critical. ..
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    ..Higher levels of salivary phosphate secretion were found in HD and in CKD patients, along with a relationship between serum phosphorus levels and a high phosphate content of beverages in HD patients. ..
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    ..Alternatively, DING proteins could be encoded by eukaryotic genes sharing special features that prevent their cloning. Both hypotheses are discussed. ..
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    ..These and the other findings suggest that the Pho86p and Pho87p proteins collaborate with Pho84p in Pi uptake. ..
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    ..Suppression increases orfA-pit expression compensating for the deficiencies in phosphate assimilation and symbiosis of the phoCDET mutants...
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    ..Southern analysis indicated that tobacco phosphate transporter genes are low copy number genes and members of a small multi-gene family. ..
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