nucleocytoplasmic transport proteins

Summary

Summary: Proteins involved in the process of transporting molecules in and out the cell nucleus. Included here are: NUCLEOPORINS, which are membrane proteins that form the NUCLEAR PORE COMPLEX; KARYOPHERINS, which carry molecules through the nuclear pore complex; and proteins that play a direct role in the transport of karyopherin complexes through the nuclear pore complex.

Top Publications

  1. Qu X, Lykke Andersen S, Nasser T, Saguez C, Bertrand E, Jensen T, et al. Assembly of an export-competent mRNP is needed for efficient release of the 3'-end processing complex after polyadenylation. Mol Cell Biol. 2009;29:5327-38 pubmed publisher
    ..Our results indicate a function for nuclear mRNP assembly factors in releasing the 3'-end processing complex once polyadenylation is complete. ..
  2. Chekanova J, Abruzzi K, Rosbash M, Belostotsky D. Sus1, Sac3, and Thp1 mediate post-transcriptional tethering of active genes to the nuclear rim as well as to non-nascent mRNP. RNA. 2008;14:66-77 pubmed
    ..Taken together with other recent findings, these observations also highlight the importance of nuclear mRNP to the mobilization of active genes to the nuclear rim. ..
  3. Kelly S, Pabit S, Kitchen C, Guo P, Marfatia K, Murphy T, et al. Recognition of polyadenosine RNA by zinc finger proteins. Proc Natl Acad Sci U S A. 2007;104:12306-11 pubmed
  4. Takano K, Miki T, Katahira J, Yoneda Y. NXF2 is involved in cytoplasmic mRNA dynamics through interactions with motor proteins. Nucleic Acids Res. 2007;35:2513-21 pubmed
    ..These results suggest that NXF2, a nucleo-cytoplasmic mRNA transporter, plays additional roles in the cytoplasmic localization of mRNAs through interactions with cytoplasmic motor proteins. ..
  5. Wickramasinghe V, McMurtrie P, Mills A, Takei Y, Penrhyn Lowe S, Amagase Y, et al. mRNA export from mammalian cell nuclei is dependent on GANP. Curr Biol. 2010;20:25-31 pubmed publisher
    ..These results establish GANP as an integral component of the mammalian mRNA export machinery and suggest a model whereby GANP facilitates the transfer of NXF1-containing mRNPs to NPCs. ..
  6. Nousiainen H, Kestila M, Pakkasjärvi N, Honkala H, Kuure S, Tallila J, et al. Mutations in mRNA export mediator GLE1 result in a fetal motoneuron disease. Nat Genet. 2008;40:155-7 pubmed publisher
    ..Our finding of mutated GLE1 exposes a common pathway connecting the genes implicated in LCCS1, LCCS2 and LCCS3 and elucidates mRNA processing as a critical molecular mechanism in motoneuron development and maturation. ..
  7. Grant R, Marshall N, Yang J, Fasken M, Kelly S, Harreman M, et al. Structure of the N-terminal Mlp1-binding domain of the Saccharomyces cerevisiae mRNA-binding protein, Nab2. J Mol Biol. 2008;376:1048-59 pubmed publisher
  8. Johnson L, Li L, Sandri Goldin R. The cellular RNA export receptor TAP/NXF1 is required for ICP27-mediated export of herpes simplex virus 1 RNA, but the TREX complex adaptor protein Aly/REF appears to be dispensable. J Virol. 2009;83:6335-46 pubmed publisher
    ..In contrast, a decrease in TAP/NXF1 levels severely impaired export of ICP27 and poly(A)(+) RNA. We conclude that TAP/NXF1 is essential for ICP27-mediated export of RNA during HSV-1 infection, whereas Aly/REF may be dispensable. ..
  9. Kitao S, Segref A, Kast J, Wilm M, Mattaj I, Ohno M. A compartmentalized phosphorylation/dephosphorylation system that regulates U snRNA export from the nucleus. Mol Cell Biol. 2008;28:487-97 pubmed
    ..This finding was surprising in that such a specific system for U snRNA export regulation is composed of two such universal regulators, suggesting that this compartmentalized system is used more broadly for gene expression regulation. ..

More Information

Publications100

  1. Marnef A, Weil D, Standart N. RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor. Mol Biol Cell. 2012;23:213-24 pubmed publisher
    ..Taken together, our findings strongly suggest that Pat1b participates in several RNA-related nuclear processes in addition to its multiple regulatory roles in the cytoplasm. ..
  2. Thakurta A, Selvanathan S, Patterson A, Gopal G, Dhar R. The nuclear export signal of splicing factor Uap56p interacts with nuclear pore-associated protein Rae1p for mRNA export in Schizosaccharomyces pombe. J Biol Chem. 2007;282:17507-16 pubmed
    ..RNA binding and the ability to shuttle between the nucleus and cytoplasm are important features of mRNA export carriers such as HIV-Rev. Our results suggest that Uap56p could function similarly as an export carrier of mRNA in S. pombe. ..
  3. Andrei M, Ingelfinger D, Heintzmann R, Achsel T, Rivera Pomar R, Luhrmann R. A role for eIF4E and eIF4E-transporter in targeting mRNPs to mammalian processing bodies. RNA. 2005;11:717-27 pubmed
    ..These data support a model in which mRNPs undergo several successive steps of remodeling and/or 3' trimming until their composition or structural organization promotes their accumulation in P bodies. ..
  4. Coyle J, Bor Y, Rekosh D, Hammarskjold M. The Tpr protein regulates export of mRNAs with retained introns that traffic through the Nxf1 pathway. RNA. 2011;17:1344-56 pubmed publisher
    ..Also, no effects were observed on export of a completely spliced mRNA. Taken together, our results indicate that Tpr plays an important role in quality control of mRNA trafficked on the Nxf1 pathway. ..
  5. Ferraiuolo M, Basak S, Dostie J, Murray E, Schoenberg D, Sonenberg N. A role for the eIF4E-binding protein 4E-T in P-body formation and mRNA decay. J Cell Biol. 2005;170:913-24 pubmed
    ..Collectively, these data suggest that 4E-T interaction with eIF4E is a priming event in inducing messenger ribonucleoprotein rearrangement and transition from translation to decay. ..
  6. Dossani Z, Weirich C, Erzberger J, Berger J, Weis K. Structure of the C-terminus of the mRNA export factor Dbp5 reveals the interaction surface for the ATPase activator Gle1. Proc Natl Acad Sci U S A. 2009;106:16251-6 pubmed publisher
  7. Blower M, Nachury M, Heald R, Weis K. A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. Cell. 2005;121:223-34 pubmed
    ..Furthermore, we provide evidence that RNA associates with the mitotic spindle and that it plays a direct, translation-independent role in spindle assembly. Our studies reveal an unexpected function for RNA in spindle morphogenesis. ..
  8. Swartz J, Bor Y, Misawa Y, Rekosh D, Hammarskjold M. The shuttling SR protein 9G8 plays a role in translation of unspliced mRNA containing a constitutive transport element. J Biol Chem. 2007;282:19844-53 pubmed
    ..These results provide further evidence for crucial links between RNA splicing, export and translation...
  9. Cargnello M, Tcherkezian J, Dorn J, Huttlin E, Maddox P, Gygi S, et al. Phosphorylation of the eukaryotic translation initiation factor 4E-transporter (4E-T) by c-Jun N-terminal kinase promotes stress-dependent P-body assembly. Mol Cell Biol. 2012;32:4572-84 pubmed publisher
  10. Umlauf D, Bonnet J, Waharte F, Fournier M, Stierlé M, Fischer B, et al. The human TREX-2 complex is stably associated with the nuclear pore basket. J Cell Sci. 2013;126:2656-67 pubmed publisher
    ..Thus, our results show that TREX-2 is an NPC-associated complex in mammalian cells and suggest that it is involved in putative NPC basket-related functions. ..
  11. Grillo L, Reitano S, Belfiore G, Spalletta A, Amata S, Bottitta M, et al. Familial 1.1 Mb deletion in chromosome Xq22.1 associated with mental retardation and behavioural disorders in female patients. Eur J Med Genet. 2010;53:113-6 pubmed publisher
    ..The dosage imbalance of NXF5 and NXF2 genes may explain the severe phenotype in our patient. ..
  12. LEVESQUE L, Bor Y, Matzat L, Jin L, Berberoglu S, Rekosh D, et al. Mutations in tap uncouple RNA export activity from translocation through the nuclear pore complex. Mol Biol Cell. 2006;17:931-43 pubmed
    ..These data suggest that Tap requires both the UBA- and NTF2-like domains to mediate the export of RNA cargo, but can move through the pores independently of these domains when free of RNA cargo...
  13. González Aguilera C, Tous C, Gómez González B, Huertas P, Luna R, Aguilera A. The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability. Mol Biol Cell. 2008;19:4310-8 pubmed publisher
  14. Estruch F, Hodge C, Gómez Navarro N, Peiró Chova L, Heath C, Cole C. Insights into mRNP biogenesis provided by new genetic interactions among export and transcription factors. BMC Genet. 2012;13:80 pubmed publisher
  15. Frey S, Richter R, Gorlich D. FG-rich repeats of nuclear pore proteins form a three-dimensional meshwork with hydrogel-like properties. Science. 2006;314:815-7 pubmed
    ..Furthermore, we obtained evidence that such hydrogel formation is required for viability in yeast. ..
  16. Ellisdon A, Dimitrova L, Hurt E, Stewart M. Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complex. Nat Struct Mol Biol. 2012;19:328-36 pubmed publisher
  17. Nojima T, Hirose T, Kimura H, Hagiwara M. The interaction between cap-binding complex and RNA export factor is required for intronless mRNA export. J Biol Chem. 2007;282:15645-51 pubmed publisher
    ..The export of Cy3-labeled intronless beta-globin mRNA from nuclei of HeLa cells was enhanced by co-injection of CBP20 and REF. Thus, REF recruited by CBP20 may play a stimulatory role to export the capped intronless mRNAs...
  18. Wohlwend D, Strasser A, Dickmanns A, Ficner R. Structural basis for RanGTP independent entry of spliceosomal U snRNPs into the nucleus. J Mol Biol. 2007;374:1129-38 pubmed
  19. Unni A, Bondar T, Medzhitov R. Intrinsic sensor of oncogenic transformation induces a signal for innate immunosurveillance. Proc Natl Acad Sci U S A. 2008;105:1686-91 pubmed publisher
    ..This finding suggests that the regulation of NKG2D ligands depends on a mechanism for intrinsic sensing of oncogenic transformation. ..
  20. Tintaru A, Hautbergue G, Hounslow A, Hung M, Lian L, Craven C, et al. Structural and functional analysis of RNA and TAP binding to SF2/ASF. EMBO Rep. 2007;8:756-62 pubmed
    ..The linker connecting RRM1 and RRM2 contains arginine residues, which provide a binding site for the mRNA export factor TAP, and when TAP binds to this region it displaces RNA bound to RRM2. ..
  21. Perrera C, Colombo R, Valsasina B, Carpinelli P, Troiani S, Modugno M, et al. Identification of Myb-binding protein 1A (MYBBP1A) as a novel substrate for aurora B kinase. J Biol Chem. 2010;285:11775-85 pubmed publisher
    ..The results presented herein show MYBBP1A as a novel Aurora B kinase substrate and reveal a not yet recognized link of this nucleolar protein to mitosis. ..
  22. Hodge C, Tran E, Noble K, Alcázar Román A, Ben Yishay R, Scarcelli J, et al. The Dbp5 cycle at the nuclear pore complex during mRNA export I: dbp5 mutants with defects in RNA binding and ATP hydrolysis define key steps for Nup159 and Gle1. Genes Dev. 2011;25:1052-64 pubmed publisher
    ..This work reveals critical steps in the Gle1-IP(6)/Dbp5/Nup159 cycle, and suggests that the number of remodeling events mediated by a single Dbp5 is limited. ..
  23. Yao W, Roser D, Köhler A, Bradatsch B, Bassler J, Hurt E. Nuclear export of ribosomal 60S subunits by the general mRNA export receptor Mex67-Mtr2. Mol Cell. 2007;26:51-62 pubmed
    ..Thus, the general mRNA exporter Mex67-Mtr2 contains a distinct electrostatic interaction surface for transporting 60S preribosomal cargo. ..
  24. Villaescusa J, Allard P, Carminati E, Kontogiannea M, Talarico D, Blasi F, et al. Clast4, the murine homologue of human eIF4E-Transporter, is highly expressed in developing oocytes and post-translationally modified at meiotic maturation. Gene. 2006;367:101-9 pubmed
    ..Our results suggest that Clast4, similar to Drosophila Cup, may act at the translational level during murine female germ-line development. ..
  25. Noble K, Tran E, Alcázar Román A, Hodge C, Cole C, Wente S. The Dbp5 cycle at the nuclear pore complex during mRNA export II: nucleotide cycling and mRNP remodeling by Dbp5 are controlled by Nup159 and Gle1. Genes Dev. 2011;25:1065-77 pubmed publisher
    ..We propose a model wherein Nup159 and Gle1-IP(6) regulate Dbp5 cycles by controlling its nucleotide-bound state, allowing multiple cycles of mRNP remodeling by a single Dbp5 at the NPC...
  26. Hendriksen J, Fagotto F, van der Velde H, van Schie M, Noordermeer J, Fornerod M. RanBP3 enhances nuclear export of active (beta)-catenin independently of CRM1. J Cell Biol. 2005;171:785-97 pubmed
    ..We conclude that RanBP3 is a direct export enhancer for beta-catenin, independent of its role as a CRM1-associated nuclear export cofactor. ..
  27. Hargous Y, Hautbergue G, Tintaru A, Skrisovska L, Golovanov A, Stevenin J, et al. Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8. EMBO J. 2006;25:5126-37 pubmed
    ..Together, these results provide a molecular description for mRNA and TAP recognition by SRp20 and 9G8. ..
  28. Napetschnig J, Kassube S, Debler E, Wong R, Blobel G, Hoelz A. Structural and functional analysis of the interaction between the nucleoporin Nup214 and the DEAD-box helicase Ddx19. Proc Natl Acad Sci U S A. 2009;106:3089-94 pubmed publisher
    ..Repeat of these cycles would remove proteins from a mRNP, one at a time, akin to a ratchet mechanism for mRNA export. ..
  29. Dieppois G, Iglesias N, Stutz F. Cotranscriptional recruitment to the mRNA export receptor Mex67p contributes to nuclear pore anchoring of activated genes. Mol Cell Biol. 2006;26:7858-70 pubmed
  30. Suzuki T, Izumi H, Ohno M. Cajal body surveillance of U snRNA export complex assembly. J Cell Biol. 2010;190:603-12 pubmed publisher
    ..Similar results were obtained with U snRNAs transcribed from microinjected genes. These results reveal a novel function for CBs, which ensure that U snRNA precursors are properly bound by PHAX. ..
  31. Collins R, Karlberg T, Lehtiö L, Schütz P, van den Berg S, Dahlgren L, et al. The DEXD/H-box RNA helicase DDX19 is regulated by an {alpha}-helical switch. J Biol Chem. 2009;284:10296-300 pubmed publisher
    ..This is the first study describing crystal structures of a DEXD/H-box protein in its open and closed cleft conformations. ..
  32. Tan W, Zolotukhin A, Tretyakova I, Bear J, Lindtner S, Smulevitch S, et al. Identification and characterization of the mouse nuclear export factor (Nxf) family members. Nucleic Acids Res. 2005;33:3855-65 pubmed
    ..We concluded that mNXF2 is an active mRNA export receptor similar to the prototype TAP/hNXF1, whereas mNXF7 may have a more specialized role in the cytoplasm. ..
  33. Fan J, Cheng Z, Zhang J, Noble C, Zhou Z, Song H, et al. Solution and crystal structures of mRNA exporter Dbp5p and its interaction with nucleotides. J Mol Biol. 2009;388:1-10 pubmed publisher
    ..Binding of ATP or ADP to NTD induces significant conformational rearrangement too. ..
  34. Schmid M, Poulsen M, Olszewski P, Pelechano V, Saguez C, Gupta I, et al. Rrp6p controls mRNA poly(A) tail length and its decoration with poly(A) binding proteins. Mol Cell. 2012;47:267-80 pubmed publisher
    ..We suggest that a nuclear mRNP surveillance step involves targeting of Rrp6p by Nab2p-bound pA-tailed RNPs and that pre-mRNA abundance is regulated at this level. ..
  35. Katahira J, Miki T, Takano K, Maruhashi M, Uchikawa M, Tachibana T, et al. Nuclear RNA export factor 7 is localized in processing bodies and neuronal RNA granules through interactions with shuttling hnRNPs. Nucleic Acids Res. 2008;36:616-28 pubmed
    ..These findings suggest that NXF7 plays a role in sorting, transport and/or storage of mRNAs through interactions with hnRNP A3...
  36. Alcázar Román A, Tran E, Guo S, Wente S. Inositol hexakisphosphate and Gle1 activate the DEAD-box protein Dbp5 for nuclear mRNA export. Nat Cell Biol. 2006;8:711-6 pubmed
    ..We now propose that Dbp5 activation at NPCs requires Gle1 and InsP6. This would facilitate spatial control of the remodelling of mRNP protein composition during directional transport and provide energy to power transport cycles. ..
  37. Paradise A, Levin M, Korza G, Carson J. Significant proportions of nuclear transport proteins with reduced intracellular mobilities resolved by fluorescence correlation spectroscopy. J Mol Biol. 2007;365:50-65 pubmed
    ..Variation in concentrations of freely diffusing nuclear transport intermediates among cells indicates that the nuclear transport system is sufficiently robust to function over a wide range of conditions. ..
  38. Cook A, Bono F, Jinek M, Conti E. Structural biology of nucleocytoplasmic transport. Annu Rev Biochem. 2007;76:647-71 pubmed
  39. Tutucci E, Stutz F. Keeping mRNPs in check during assembly and nuclear export. Nat Rev Mol Cell Biol. 2011;12:377-84 pubmed publisher
  40. McGuire A, Mangroo D. Cex1p is a novel cytoplasmic component of the Saccharomyces cerevisiae nuclear tRNA export machinery. EMBO J. 2007;26:288-300 pubmed
    ..cerevisiae. They also suggest that Cex1p collects aminoacyl-tRNAs from the nuclear export receptors at the cytoplasmic side of the nuclear pore complex, and transfers them to eEF-1A using a channelling mechanism. ..
  41. Pan J, Eckardt S, Leu N, Buffone M, Zhou J, Gerton G, et al. Inactivation of Nxf2 causes defects in male meiosis and age-dependent depletion of spermatogonia. Dev Biol. 2009;330:167-74 pubmed publisher
    ..These studies demonstrate that Nxf2 plays a dual function in spermatogenesis: regulation of meiosis and maintenance of spermatogonial stem cells. ..
  42. Jani D, Lutz S, Marshall N, Fischer T, Köhler A, Ellisdon A, et al. Sus1, Cdc31, and the Sac3 CID region form a conserved interaction platform that promotes nuclear pore association and mRNA export. Mol Cell. 2009;33:727-37 pubmed publisher
    ..These data indicate Sac3(CID) provides a scaffold within TREX-2 to integrate interactions between protein complexes to facilitate the coupling of transcription and mRNA export during gene expression...
  43. Soucek S, Corbett A, Fasken M. The long and the short of it: the role of the zinc finger polyadenosine RNA binding protein, Nab2, in control of poly(A) tail length. Biochim Biophys Acta. 2012;1819:546-54 pubmed publisher
    ..This article is part of a Special Issue entitled: Nuclear Transport and RNA Processing. ..
  44. Dai F, Lin X, Chang C, Feng X. Nuclear export of Smad2 and Smad3 by RanBP3 facilitates termination of TGF-beta signaling. Dev Cell. 2009;16:345-57 pubmed publisher
    ..In conclusion, our study supports a definitive role for RanBP3 in mediating Smad2/3 nuclear export and terminating TGF-beta signaling. ..
  45. McCloskey A, Taniguchi I, Shinmyozu K, Ohno M. hnRNP C tetramer measures RNA length to classify RNA polymerase II transcripts for export. Science. 2012;335:1643-6 pubmed publisher
    ..Our findings reveal a new function of the C tetramer and highlight the biological importance of RNA recognition by the length. ..
  46. Murthi A, Shaheen H, Huang H, Preston M, Lai T, Phizicky E, et al. Regulation of tRNA bidirectional nuclear-cytoplasmic trafficking in Saccharomyces cerevisiae. Mol Biol Cell. 2010;21:639-49 pubmed publisher
    ..Finally, we implicate Tef1, the yeast orthologue of translation elongation factor eEF1A, in the tRNA reexport process and show that its subcellular distribution between the nucleus and cytoplasm is dependent upon Mtr10 and Msn5. ..
  47. Read E, Digard P. Individual influenza A virus mRNAs show differential dependence on cellular NXF1/TAP for their nuclear export. J Gen Virol. 2010;91:1290-301 pubmed publisher
    ..We conclude that influenza A virus co-opts the main cellular mRNA export pathway for a subset of its mRNAs, including most but not all late gene transcripts. ..
  48. Scarcelli J, Viggiano S, Hodge C, Heath C, Amberg D, Cole C. Synthetic genetic array analysis in Saccharomyces cerevisiae provides evidence for an interaction between RAT8/DBP5 and genes encoding P-body components. Genetics. 2008;179:1945-55 pubmed publisher
    ..Although these foci are distinct from P-bodies, the two merge under heat-stress conditions. We suggest that these granules reflect defective mRNP remodeling during mRNA export and during cytoplasmic mRNA metabolism. ..
  49. Gross T, Siepmann A, Sturm D, Windgassen M, Scarcelli J, Seedorf M, et al. The DEAD-box RNA helicase Dbp5 functions in translation termination. Science. 2007;315:646-9 pubmed
    ..Therefore, Dbp5 controls the eRF3-eRF1 interaction and thus eRF3-mediated downstream events. ..
  50. Batisse J, Batisse C, Budd A, Bottcher B, Hurt E. Purification of nuclear poly(A)-binding protein Nab2 reveals association with the yeast transcriptome and a messenger ribonucleoprotein core structure. J Biol Chem. 2009;284:34911-7 pubmed publisher
    ..Electron microscopy revealed that many of the mRNPs have a characteristic elongated structure. Our data suggest that mRNPs, although associated with different mRNAs, have a unifying core structure. ..
  51. Ren Y, Seo H, Blobel G, Hoelz A. Structural and functional analysis of the interaction between the nucleoporin Nup98 and the mRNA export factor Rae1. Proc Natl Acad Sci U S A. 2010;107:10406-11 pubmed publisher
    ..Together, these data suggest that the Rae1*Nup98 complex directly binds to the mRNP at several stages of the mRNA export pathway. ..
  52. Uranishi H, Zolotukhin A, Lindtner S, Warming S, Zhang G, Bear J, et al. The RNA-binding motif protein 15B (RBM15B/OTT3) acts as cofactor of the nuclear export receptor NXF1. J Biol Chem. 2009;284:26106-16 pubmed publisher
  53. Zhang M, Wang Q, Huang Y. Fragile X mental retardation protein FMRP and the RNA export factor NXF2 associate with and destabilize Nxf1 mRNA in neuronal cells. Proc Natl Acad Sci U S A. 2007;104:10057-62 pubmed
    ..Such regulation may prove important in the normal development and function of neurons as well as of male germ cells. ..
  54. Frey S, Gorlich D. A saturated FG-repeat hydrogel can reproduce the permeability properties of nuclear pore complexes. Cell. 2007;130:512-23 pubmed
    ..Intragel diffusion of the importin beta-cargo complex occurred rapidly enough to traverse an NPC within approximately 12 ms. We extend the "selective phase model" to explain these effects. ..
  55. Matzat L, Berberoglu S, LEVESQUE L. Formation of a Tap/NXF1 homotypic complex is mediated through the amino-terminal domain of Tap and enhances interaction with nucleoporins. Mol Biol Cell. 2008;19:327-38 pubmed
    ..This is the first report showing that the Tap amino terminus modulates the interaction of Tap with nucleoporins. We speculate that this mechanism has a regulatory role for RNA export independent of RNA binding. ..
  56. Mart nez Lumbreras S, Santiveri C, Mirassou Y, Zorrilla S, P rez Ca adillas J. Two singular types of CCCH tandem zinc finger in Nab2p contribute to polyadenosine RNA recognition. Structure. 2013;21:1800-11 pubmed publisher
    ..Moreover, we describe a hypothetical mechanism for controlling poly(A) tail length with specific roles for TZF12, TZF34, and Zf5-7 domains...
  57. Sasaki M, Takeda E, Takano K, Yomogida K, Katahira J, Yoneda Y. Molecular cloning and functional characterization of mouse Nxf family gene products. Genomics. 2005;85:641-53 pubmed
    ..The orthologous relationship between human and mouse Nxf genes is discussed on the basis of these data. ..
  58. Hung M, Hautbergue G, Snijders A, Dickman M, Wilson S. Arginine methylation of REF/ALY promotes efficient handover of mRNA to TAP/NXF1. Nucleic Acids Res. 2010;38:3351-61 pubmed publisher
    ..Therefore, arginine methylation fine-tunes the RNA-binding activity of REF such that the RNA-protein interaction can be readily disrupted by export factors further down the pathway. ..
  59. Kelly S, Leung S, Apponi L, Bramley A, Tran E, Chekanova J, et al. Recognition of polyadenosine RNA by the zinc finger domain of nuclear poly(A) RNA-binding protein 2 (Nab2) is required for correct mRNA 3'-end formation. J Biol Chem. 2010;285:26022-32 pubmed publisher
    ..Together, these data provide strong evidence that Nab2 binding to RNA is critical for proper control of poly(A) tail length. ..
  60. Lei H, Zhai B, Yin S, Gygi S, Reed R. Evidence that a consensus element found in naturally intronless mRNAs promotes mRNA export. Nucleic Acids Res. 2013;41:2517-25 pubmed publisher
    ..Moreover, knockdown of these factors results in nuclear retention of the intronless mRNAs. Together, these data suggest that the CAR-E promotes export of intronless mRNA by sequence-dependent recruitment of the mRNA export machinery. ..
  61. Zhou J, Pan J, Eckardt S, Leu N, McLaughlin K, Wang P. Nxf3 is expressed in Sertoli cells, but is dispensable for spermatogenesis. Mol Reprod Dev. 2011;78:241-9 pubmed publisher
    ..With its unique expression pattern in the testis, the promoter of Nxf3 can be used to drive postnatal Sertoli cell-specific expression of other proteins such as Cre recombinase. ..
  62. Yao W, Lutzmann M, Hurt E. A versatile interaction platform on the Mex67-Mtr2 receptor creates an overlap between mRNA and ribosome export. EMBO J. 2008;27:6-16 pubmed
    ..Thus, the Mex67-Mtr2 export receptor employs a versatile binding platform on its surface that could create a crosstalk between mRNA and ribosome export pathways. ..
  63. Kendirgi F, Rexer D, Alcázar Román A, Onishko H, Wente S. Interaction between the shuttling mRNA export factor Gle1 and the nucleoporin hCG1: a conserved mechanism in the export of Hsp70 mRNA. Mol Biol Cell. 2005;16:4304-15 pubmed
    ..Because this closely parallels the role of the hCG1 orthologue yNup42/Rip1, we speculate that hGle1-hCG1 function in the mRNA export mechanism is highly conserved. ..
  64. Tretyakova I, Zolotukhin A, Tan W, Bear J, Propst F, Ruthel G, et al. Nuclear export factor family protein participates in cytoplasmic mRNA trafficking. J Biol Chem. 2005;280:31981-90 pubmed
    ..We propose a model in which MAP1B tethers the NXF-associated mRNA to microtubules and facilitates their translocation along dendrites while Unrip provides a scaffold for the assembly of these transport intermediates. ..
  65. Lai D, Sakkas D, Huang Y. The fragile X mental retardation protein interacts with a distinct mRNA nuclear export factor NXF2. RNA. 2006;12:1446-9 pubmed
    ..We thus hypothesize that FMRP and NXF2 may act in concert to promote the nucleocytoplasmic transport of specific mRNAs in male germ cells and neurons. ..
  66. Culjkovic B, Topisirovic I, Skrabanek L, Ruiz Gutierrez M, Borden K. eIF4E is a central node of an RNA regulon that governs cellular proliferation. J Cell Biol. 2006;175:415-26 pubmed
    ..Finally, the growth-suppressive promyelocytic leukemia protein (PML) inhibits this RNA regulon. These data provide novel perspectives into the proliferative and oncogenic properties of eIF4E. ..
  67. Farny N, Hurt J, Silver P. Definition of global and transcript-specific mRNA export pathways in metazoans. Genes Dev. 2008;22:66-78 pubmed
    ..Our results define the global network of factors involved in Drosophila mRNA export, reveal specificity in the export requirements of different transcripts, and expose new avenues for future work in mRNA export. ..
  68. Van Impe K, Hubert T, De Corte V, Vanloo B, Boucherie C, Vandekerckhove J, et al. A new role for nuclear transport factor 2 and Ran: nuclear import of CapG. Traffic. 2008;9:695-707 pubmed publisher
    ..Thus, a ubiquitously expressed protein shuttles to the nucleus through direct association with NTF2 and Ran. The role of NTF2 may therefore not be solely confined to sustaining the Ran gradient in cells. ..
  69. Yoon S, Shin S, Liu Y, Ballif B, Woo M, Gygi S, et al. Ran-binding protein 3 phosphorylation links the Ras and PI3-kinase pathways to nucleocytoplasmic transport. Mol Cell. 2008;29:362-75 pubmed publisher
    ..Our findings highlight an important link between two major cell-fate determinants: nuclear transport and the Ras/ERK/RSK and PI3K/Akt signaling pathways. ..
  70. Lange A, Mills R, Devine S, Corbett A. A PY-NLS nuclear targeting signal is required for nuclear localization and function of the Saccharomyces cerevisiae mRNA-binding protein Hrp1. J Biol Chem. 2008;283:12926-34 pubmed publisher
    ..Finally, we apply this analysis to a bioinformatic search of the yeast proteome as a preliminary search for new potential Kap104 cargos. ..
  71. Hautbergue G, Hung M, Golovanov A, Lian L, Wilson S. Mutually exclusive interactions drive handover of mRNA from export adaptors to TAP. Proc Natl Acad Sci U S A. 2008;105:5154-9 pubmed publisher
    ..The importance of direct TAP-mRNA interactions is confirmed by the observation that a mutant form of TAP that fails to bind mRNA but retains the ability to bind REF does not function in mRNA export. ..
  72. Lee H, Cho H, Kim Y. Ectopic expression of eIF4E-transporter triggers the movement of eIF4E into P-bodies, inhibiting steady-state translation but not the pioneer round of translation. Biochem Biophys Res Commun. 2008;369:1160-5 pubmed publisher
    ..These results suggest that the pioneer round of translation differs from steady-state translation in terms of ribosome recruitment. ..
  73. Roth K, Byam J, Fang F, Butler J. Regulation of NAB2 mRNA 3'-end formation requires the core exosome and the Trf4p component of the TRAMP complex. RNA. 2009;15:1045-58 pubmed publisher
    ..These findings suggest that NAB2 mRNA 3'-end formation requires the exosome and TRAMP complex, and that competition between polyadenylation and Rrp6p-dependent degradation controls the level of this mRNA. ..
  74. Wilmes G, Guthrie C. Getting to the gate: crystallization of a Sac3(CID):Sus1:Cdc31 complex. Mol Cell. 2009;33:671-2 pubmed publisher
    ..2009) present the crystal structure of a region of TREX2, thereby providing a physical framework for how the transcription and export machineries might cooperate to "gate" genes to the nuclear pore. ..
  75. Palancade B, Zuccolo M, Loeillet S, Nicolas A, Doye V. Pml39, a novel protein of the nuclear periphery required for nuclear retention of improper messenger ribonucleoparticles. Mol Biol Cell. 2005;16:5258-68 pubmed
    ..Together, these data indicate that Pml39 is an upstream effector of the Mlps, involved in the retention of improper mRNPs in the nucleus before their export. ..
  76. Jeganathan K, Malureanu L, van Deursen J. The Rae1-Nup98 complex prevents aneuploidy by inhibiting securin degradation. Nature. 2005;438:1036-9 pubmed
    ..Together, our results suggest that Rae1 and Nup98 are temporal regulators of APC(Cdh1) that maintain euploidy by preventing unscheduled degradation of securin. ..
  77. Viphakone N, Hautbergue G, Walsh M, Chang C, Holland A, Folco E, et al. TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export. Nat Commun. 2012;3:1006 pubmed publisher
    ..Together, our data indicate that TREX provides a license for mRNA export by driving Nxf1 into a conformation capable of binding mRNA. ..
  78. Weirich C, Erzberger J, Flick J, Berger J, Thorner J, Weis K. Activation of the DExD/H-box protein Dbp5 by the nuclear-pore protein Gle1 and its coactivator InsP6 is required for mRNA export. Nat Cell Biol. 2006;8:668-76 pubmed
    ..Our results define specific functions for Gle1 and InsP6 in mRNA export and suggest that local activation of Dbp5 at the nuclear pore is critical for mRNA export. ..
  79. Cole C, Scarcelli J. Unravelling mRNA export. Nat Cell Biol. 2006;8:645-7 pubmed
  80. Lindtner S, Zolotukhin A, Uranishi H, Bear J, Kulkarni V, Smulevitch S, et al. RNA-binding motif protein 15 binds to the RNA transport element RTE and provides a direct link to the NXF1 export pathway. J Biol Chem. 2006;281:36915-28 pubmed
    ..We propose that RTE evolved as a high affinity RBM15 ligand to provide a splicing-independent link to NXF1, thereby ensuring efficient nuclear export and expression of retrotransposon transcripts. ..
  81. Acuña Sanhueza G, Faller L, George B, Koffler J, Misetic V, Flechtenmacher C, et al. Opposing function of MYBBP1A in proliferation and migration of head and neck squamous cell carcinoma cells. BMC Cancer. 2012;12:72 pubmed publisher
  82. Pettit Kneller E, Connor J, Lyles D. hnRNPs Relocalize to the cytoplasm following infection with vesicular stomatitis virus. J Virol. 2009;83:770-80 pubmed publisher
    ..These data suggest that VSV promotes hnRNPA1 relocalization in a Rae1-dependent manner for apoptotic signaling. ..
  83. Tillemans V, Leponce I, Rausin G, Dispa L, Motte P. Insights into nuclear organization in plants as revealed by the dynamic distribution of Arabidopsis SR splicing factors. Plant Cell. 2006;18:3218-34 pubmed
    ..Our findings emphasize the high mobility of Arabidopsis SR splicing factors and provide insights into the dynamic relationships between the different nuclear compartments. ..
  84. Wang W, Cui Z, Han H, Zhang Z, Wei H, Zhou Y, et al. Imaging and characterizing influenza A virus mRNA transport in living cells. Nucleic Acids Res. 2008;36:4913-28 pubmed publisher
    ..These findings characterize influenza A virus mRNA transport in living cells and suggest that influenza A virus mRNA may be exported from the nucleus by the cellular TAP/p15 pathway with NS1 protein and RNAP-II participation. ..
  85. Yamauchi T, Keough R, Gonda T, Ishii S. Ribosomal stress induces processing of Mybbp1a and its translocation from the nucleolus to the nucleoplasm. Genes Cells. 2008;13:27-39 pubmed publisher
    ..These results suggest that Mybbp1a may connect the ribosome biogenesis and the Myb-dependent transcription, which controls cell cycle progression and proliferation. ..
  86. Kuroda T, Murayama A, Katagiri N, Ohta Y, Fujita E, Masumoto H, et al. RNA content in the nucleolus alters p53 acetylation via MYBBP1A. EMBO J. 2011;30:1054-66 pubmed publisher
    ..Our observations indicated that a dynamic equilibrium between RNA generation and export regulated nucleolar RNA content. Perturbation of this balance by nucleolar stress altered the nucleolar RNA content and modulated p53 activity...
  87. Lowe A, Siegel J, Kalab P, Siu M, Weis K, LIPHARDT J. Selectivity mechanism of the nuclear pore complex characterized by single cargo tracking. Nature. 2010;467:600-3 pubmed publisher
    ..The overall selectivity of the NPC seems to arise from the cumulative action of multiple reversible substeps and a final irreversible exit step. ..
  88. von Moeller H, Basquin C, Conti E. The mRNA export protein DBP5 binds RNA and the cytoplasmic nucleoporin NUP214 in a mutually exclusive manner. Nat Struct Mol Biol. 2009;16:247-54 pubmed publisher
    ..More generally, they provide a paradigm for how binding of regulators can specifically inhibit DEAD-box proteins. ..
  89. Dong S, Li C, Zenklusen D, Singer R, Jacobson A, He F. YRA1 autoregulation requires nuclear export and cytoplasmic Edc3p-mediated degradation of its pre-mRNA. Mol Cell. 2007;25:559-73 pubmed
  90. Herrmann M, Neuberth N, Wissler J, Perez J, Gradl D, Naber A. Near-field optical study of protein transport kinetics at a single nuclear pore. Nano Lett. 2009;9:3330-6 pubmed publisher
    ..NSOM-FCS may help to understand the facilitated translocation in more detail and offers a new way to study single molecule mobility on a nanoscale...
  91. Minakhina S, Myers R, Druzhinina M, Steward R. Crosstalk between the actin cytoskeleton and Ran-mediated nuclear transport. BMC Cell Biol. 2005;6:32 pubmed
    ..We show that in chic mutants, as in Sd-RanGAP, nuclear export is impaired. Our data suggest that Profilin and the organization of the actin cytoskeleton play an important role in nuclear trafficking. ..