voltage dependent anion channel 1


Summary: Voltage-dependent anion channel 1 is the major pore-forming protein of the mitochondrial outer membrane. It also functions as a ferricyanide reductase in the PLASMA MEMBRANE.

Top Publications

  1. Chen Y, Gaczynska M, Osmulski P, Polci R, Riley D. Phosphorylation by Nek1 regulates opening and closing of voltage dependent anion channel 1. Biochem Biophys Res Commun. 2010;394:798-803 pubmed publisher
    ..Our data provide a mechanism to explain how Nek1 regulates cell death by affecting the opening and closing of VDAC1. ..
  2. Shimada H, Hirai K, Simamura E, Hatta T, Iwakiri H, Mizuki K, et al. Paraquat toxicity induced by voltage-dependent anion channel 1 acts as an NADH-dependent oxidoreductase. J Biol Chem. 2009;284:28642-9 pubmed publisher
    ..These insights into the mechanism of PQ poisoning not only demonstrated novel physiological functions of VDAC protein, but they may facilitate the development of new therapeutic approaches. ..
  3. Serrano Pozo A, William C, Ferrer I, Uro Coste E, Delisle M, Maurage C, et al. Beneficial effect of human anti-amyloid-beta active immunization on neurite morphology and tau pathology. Brain. 2010;133:1312-27 pubmed publisher
  4. Groebe K, Klemm Manns M, Schwall G, Hübenthal H, Unterluggauer H, Jansen Durr P, et al. Age-dependent posttranslational modifications of voltage-dependent anion channel 1. Exp Gerontol. 2010;45:632-7 pubmed publisher
    ..We show that glutamate-induced calcium toxicity in neurons induces changes of voltage-dependent anion channel 1 related to downstream events of mitochondrial apoptosis like poly-ADP-ribosylation. ..
  5. Poleti M, Tesch A, Crepaldi C, Souza G, Eberlin M, de Cerqueira Cesar M. Relationship between expression of voltage-dependent anion channel (VDAC) isoforms and type of hexokinase binding sites on brain mitochondria. J Mol Neurosci. 2010;41:48-54 pubmed publisher
    ..Bovine brain mitochondria had the highest VDAC2 levels. We concluded that the nature of hexokinase binding site is not determined by the expression of a single VDAC isoform. ..
  6. Kielar C, Wishart T, Palmer A, Dihanich S, Wong A, Macauley S, et al. Molecular correlates of axonal and synaptic pathology in mouse models of Batten disease. Hum Mol Genet. 2009;18:4066-80 pubmed publisher
  7. Ryan C, Souda P, Bassilian S, Ujwal R, Zhang J, Abramson J, et al. Post-translational modifications of integral membrane proteins resolved by top-down Fourier transform mass spectrometry with collisionally activated dissociation. Mol Cell Proteomics. 2010;9:791-803 pubmed publisher
  8. Kozjak Pavlovic V, Ross K, Götz M, Goosmann C, Rudel T. A tag at the carboxy terminus prevents membrane integration of VDAC1 in mammalian mitochondria. J Mol Biol. 2010;397:219-32 pubmed publisher
    ..This effect offers easy tools for studying the requirements for the membrane assembly of beta-barrel proteins, but also advises caution when interpreting the outcome of the beta-barrel protein overexpression experiments. ..
  9. Lan C, Sheng J, Fang D, Meng Q, Fan L, Huang Z. Involvement of VDAC1 and Bcl-2 family of proteins in VacA-induced cytochrome c release and apoptosis of gastric epithelial carcinoma cells. J Dig Dis. 2010;11:43-9 pubmed publisher
    ..These findings suggest that the release of cytochrome c by VacA p58 is mainly through VDAC-dependent and Bcl-2 family-dependent pathways. ..

More Information


  1. Sukumaran S, Fu N, Tin C, Wan K, Lee S, Yu V. A soluble form of the pilus protein FimA targets the VDAC-hexokinase complex at mitochondria to suppress host cell apoptosis. Mol Cell. 2010;37:768-83 pubmed publisher
    ..Together, these data thus reveal a paradigm of antiapoptosis mechanism undertaken by the enteroinvasive bacteria...
  2. Weber Lotfi F, Ibrahim N, Boesch P, Cosset A, Konstantinov Y, Lightowlers R, et al. Developing a genetic approach to investigate the mechanism of mitochondrial competence for DNA import. Biochim Biophys Acta. 2009;1787:320-7 pubmed publisher
    ..cerevisiae strains deleted for the VDAC-1 or VDAC-2 gene are severely compromised in DNA import. The prospect is now open to screen further mutant yeast strains to identify the elusive inner membrane DNA transporter. ..
  3. Guo Qing P, Yuan Y, Cai Gao Z, Hongling Y, Gonghua H, Yan T. A study of association between expression of hOGG1, VDAC1, HK-2 and cervical carcinoma. J Exp Clin Cancer Res. 2010;29:129 pubmed publisher
    ..hOGG1 and HK-2 might play a key role at the early stage of cervical cancer, and the findings of hOGG1 and HK-2 should be considered as a significant biomarker at the early stage of cervical cancer. ..
  4. Aram L, Geula S, Arbel N, Shoshan Barmatz V. VDAC1 cysteine residues: topology and function in channel activity and apoptosis. Biochem J. 2010;427:445-54 pubmed publisher
  5. Gałgańska H, Karachitos A, Baranek M, Budzińska M, Jordan J, Kmita H. Viability of Saccharomyces cerevisiae cells following exposure to H2O2 and protective effect of minocycline depend on the presence of VDAC. Eur J Pharmacol. 2010;643:42-7 pubmed publisher
    ..Moreover, the ability seems to be crucial for both cell viability and protective effect of minocycline. ..
  6. Arbel N, Shoshan Barmatz V. Voltage-dependent anion channel 1-based peptides interact with Bcl-2 to prevent antiapoptotic activity. J Biol Chem. 2010;285:6053-62 pubmed publisher
    ..Moreover, these findings suggest that interfering with the binding of Bcl-2 to mitochondria by VDAC1-based peptides may serve to potentiate the efficacy of conventional chemotherapeutic agents...
  7. Bonnet S, Paulin R, Sutendra G, Dromparis P, Roy M, Watson K, et al. Dehydroepiandrosterone reverses systemic vascular remodeling through the inhibition of the Akt/GSK3-{beta}/NFAT axis. Circulation. 2009;120:1231-40 pubmed publisher
    ..We suggest that the orally available DHEA might be an attractive candidate for the treatment of systemic vascular remodeling, including restenosis, and we propose a novel mechanism of action for this important hormone and drug. ..
  8. Baines C, Kaiser R, Sheiko T, Craigen W, Molkentin J. Voltage-dependent anion channels are dispensable for mitochondrial-dependent cell death. Nat Cell Biol. 2007;9:550-5 pubmed
    ..These results indicate that Vdacs are dispensable for both MPT and Bcl-2 family member-driven cell death. ..
  9. Malia T, Wagner G. NMR structural investigation of the mitochondrial outer membrane protein VDAC and its interaction with antiapoptotic Bcl-xL. Biochemistry. 2007;46:514-25 pubmed
    ..Our characterization of the VDAC/Bcl-xL complex offers initial structural insight into the role of antiapoptotic Bcl-xL in regulating apoptotic events in the mitochondrial outer membrane. ..
  10. Gredilla R, Garm C, Holm R, Bohr V, Stevnsner T. Differential age-related changes in mitochondrial DNA repair activities in mouse brain regions. Neurobiol Aging. 2010;31:993-1002 pubmed publisher
    ..Mitochondrial AP endonuclease activity increased in old animals in both brain regions. Our data suggest an important regional specific regulation of mitochondrial BER during aging. ..
  11. Hiller S, Wagner G. The role of solution NMR in the structure determinations of VDAC-1 and other membrane proteins. Curr Opin Struct Biol. 2009;19:396-401 pubmed publisher
    ..Numerous further structural and functional studies on many different membrane proteins show that solution NMR has become an important tool for membrane protein molecular biology. ..
  12. Martinez Caballero S, Dejean L, Kinnally M, Oh K, Mannella C, Kinnally K. Assembly of the mitochondrial apoptosis-induced channel, MAC. J Biol Chem. 2009;284:12235-45 pubmed publisher
    ..5-6 nm. The mitochondrial permeability data are inconsistent with formation of lipidic pores capable of transporting megadalton-sized macromolecules as observed with recombinant Bax in liposomes. ..
  13. Hiller S, Ibraghimov I, Wagner G, Orekhov V. Coupled decomposition of four-dimensional NOESY spectra. J Am Chem Soc. 2009;131:12970-8 pubmed publisher
  14. Zeth K, Thein M. Porins in prokaryotes and eukaryotes: common themes and variations. Biochem J. 2010;431:13-22 pubmed publisher
    ..Despite the lack of a clear evolutionary link between these protein families, their common principles and differences in assembly, architecture and function are summarized in the present review. ..
  15. Deniaud A, Liguori L, Blesneac I, Lenormand J, Pebay Peyroula E. Crystallization of the membrane protein hVDAC1 produced in cell-free system. Biochim Biophys Acta. 2010;1798:1540-6 pubmed publisher
    ..Although the diffraction of VDAC crystals is limited, the simplicity and the rapidity to set-up and optimize this technology are drastic advantages in comparison to other methods. ..
  16. Reina S, Palermo V, Guarnera A, Guarino F, Messina A, Mazzoni C, et al. Swapping of the N-terminus of VDAC1 with VDAC3 restores full activity of the channel and confers anti-aging features to the cell. FEBS Lett. 2010;584:2837-44 pubmed publisher
    ..This chimaera increased life span, indicating a more efficient bioenergetic metabolism and/or a better protection from ROS. ..
  17. Hiller S, Garces R, Malia T, Orekhov V, Colombini M, Wagner G. Solution structure of the integral human membrane protein VDAC-1 in detergent micelles. Science. 2008;321:1206-10 pubmed publisher
    ..NMR measurements revealed the binding sites of VDAC-1 for the Bcl-2 protein Bcl-x(L), for reduced beta-nicotinamide adenine dinucleotide, and for cholesterol. Bcl-x(L) interacts with the VDAC barrel laterally at strands 17 and 18. ..
  18. Shoshan Barmatz V, Zakar M, Rosenthal K, Abu Hamad S. Key regions of VDAC1 functioning in apoptosis induction and regulation by hexokinase. Biochim Biophys Acta. 2009;1787:421-30 pubmed publisher
    ..Displacing HK, serving as the 'guardian of the mitochondrion', from its binding site on VDAC1 may thus be exploited as an approach to cancer therapy. ..
  19. Emelyanov V. Mitochondrial porin VDAC 1 seems to be functional in rickettsial cells. Ann N Y Acad Sci. 2009;1166:38-48 pubmed publisher
    ..A dependence of rickettsiae upon essential protein(s), destined to organelle, is also viewed as a nature of their obligate endosymbiotic lifestyle. ..
  20. Geisler S, Holmström K, Skujat D, Fiesel F, Rothfuss O, Kahle P, et al. PINK1/Parkin-mediated mitophagy is dependent on VDAC1 and p62/SQSTM1. Nat Cell Biol. 2010;12:119-31 pubmed publisher
    ..Thus, our data provide functional links between PINK1, Parkin and the selective autophagy of mitochondria, which is implicated in the pathogenesis of Parkinson's disease. ..
  21. Raschle T, Hiller S, Yu T, Rice A, Walz T, Wagner G. Structural and functional characterization of the integral membrane protein VDAC-1 in lipid bilayer nanodiscs. J Am Chem Soc. 2009;131:17777-9 pubmed publisher
  22. Prins J, Park S, Lurie D. Decreased expression of the voltage-dependent anion channel in differentiated PC-12 and SH-SY5Y cells following low-level Pb exposure. Toxicol Sci. 2010;113:169-76 pubmed publisher
    ..These results demonstrate that exposure to 5 and 10microM Pb reduces VDAC transcription and expression and is associated with reduced cellular ATP levels. ..
  23. Tomasello F, Messina A, Lartigue L, Schembri L, Medina C, Reina S, et al. Outer membrane VDAC1 controls permeability transition of the inner mitochondrial membrane in cellulo during stress-induced apoptosis. Cell Res. 2009;19:1363-76 pubmed publisher
    ..Our data thus support a model of the PTP complex involving VDAC1 at the MOM, and indicate that VDAC1-dependent MPT is an upstream mechanism playing a causal role in oxidative stress-induced apoptosis. ..
  24. Ujwal R, Cascio D, Chaptal V, Ping P, Abramson J. Crystal packing analysis of murine VDAC1 crystals in a lipidic environment reveals novel insights on oligomerization and orientation. Channels (Austin). 2009;3:167-70 pubmed
    ..The physiological implications of a novel antiparallel arrangement are addressed that may clarify these conflicting biochemical data. ..
  25. Thinnes F. Neuroendocrine differentiation of LNCaP cells suggests: VDAC in the cell membrane is involved in the extrinsic apoptotic pathway. Mol Genet Metab. 2009;97:241-3 pubmed publisher
    ..Finally, it is insinuated to search for natural antibodies against type-1 porin, what in combination with other established markers might help in early diagnosis of prostate cancer. ..
  26. Abu Hamad S, Arbel N, Calo D, Arzoine L, Israelson A, Keinan N, et al. The VDAC1 N-terminus is essential both for apoptosis and the protective effect of anti-apoptotic proteins. J Cell Sci. 2009;122:1906-16 pubmed publisher
    ..These results show VDAC1 to be a component of the apoptosis machinery and offer new insight into the mechanism of cytochrome c release and how anti-apoptotic proteins regulate apoptosis and promote tumor cell survival. ..
  27. Cassará M, Menzel V, Hinsch K, Wrenzycki C, Hinsch E. Voltage-dependent anion channels 1 and 2 are expressed in porcine oocytes. Biosci Rep. 2009;30:193-200 pubmed publisher
  28. Ramanathan A, Schreiber S. Direct control of mitochondrial function by mTOR. Proc Natl Acad Sci U S A. 2009;106:22229-32 pubmed publisher
  29. Perevoshchikova I, Zorov S, Kotova E, Zorov D, Antonenko Y. Hexokinase inhibits flux of fluorescently labeled ATP through mitochondrial outer membrane porin. FEBS Lett. 2010;584:2397-402 pubmed publisher
    ..Therefore, the hexokinase-induced inhibition of the ATP flow mediated by VDAC was revealed in isolated mitochondria. ..
  30. Mader A, Abu Hamad S, Arbel N, Gutiérrez Aguilar M, Gutierr Asuilar M, Shoshan Barmatz V. Dominant-negative VDAC1 mutants reveal oligomeric VDAC1 to be the active unit in mitochondria-mediated apoptosis. Biochem J. 2010;429:147-55 pubmed publisher
    ..These results suggest that E72Q-VDAC1 has a dominant-negative effect and implies that VDAC1 homo-oligomerization, involving intermolecular interactions, might be involved in the apoptotic process. ..
  31. Choudhary O, Ujwal R, Kowallis W, Coalson R, Abramson J, Grabe M. The electrostatics of VDAC: implications for selectivity and gating. J Mol Biol. 2010;396:580-92 pubmed publisher
    ..Thus, we were able to rule out certain models for channel gating, but the true motion has yet to be determined. ..
  32. Israelson A, Arbel N, Da Cruz S, Ilieva H, Yamanaka K, Shoshan Barmatz V, et al. Misfolded mutant SOD1 directly inhibits VDAC1 conductance in a mouse model of inherited ALS. Neuron. 2010;67:575-87 pubmed publisher
    ..Taken together, our results establish a direct link between misfolded mutant SOD1 and mitochondrial dysfunction in this form of inherited ALS. ..
  33. Danishuddin M, Khan S, Khan A. Molecular interactions between mitochondrial membrane proteins and the C-terminal domain of PB1-F2: an in silico approach. J Mol Model. 2010;16:535-41 pubmed publisher
    ..Furthermore, two hydrogen bonds were predicted in both complexes PB1-F2/VDAC1 and PB1-F2/ANT3. This study reveals the molecular interactions required for PB1-F2-induced apoptosis and suggests a hypothetical model for future study. ..
  34. Hiller S, Malia T, Garces R, Orekhov V, Wagner G. Backbone and ILV side chain methyl group assignments of the integral human membrane protein VDAC-1. Biomol NMR Assign. 2010;4:29-32 pubmed publisher
    ..Here, we report sequence-specific NMR assignments for the isoform 1 of human VDAC reconstituted in lauryldimethylamine oxide (LDAO) detergent micelles. The assignments were deposited in the BMRB data base with accession number 16381. ..