Genomes and Genes
membrane transport proteins
Summary: Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.
- Whitehouse S, Gold V, Robson A, Allen W, Sessions R, Collinson I. Mobility of the SecA 2-helix-finger is not essential for polypeptide translocation via the SecYEG complex. J Cell Biol. 2012;199:919-29 pubmed publisher..The results also have important implications on the mechanism of energy transduction and the power stroke driving transport. Evidently, the 2HF is not a highly mobile transducing element of polypeptide translocation. ..
- Kell D, Dobson P, Bilsland E, Oliver S. The promiscuous binding of pharmaceutical drugs and their transporter-mediated uptake into cells: what we (need to) know and how we can do so. Drug Discov Today. 2013;18:218-39 pubmed publisher..This needs to be acquired in cells that contain the relevant proteins, and we highlight an experimental system for simultaneous genome-wide assessment of carrier-mediated uptake in a eukaryotic cell (yeast). ..
- Di Cosmo C, Liao X, Ye H, Ferrara A, Weiss R, Refetoff S, et al. Mct8-deficient mice have increased energy expenditure and reduced fat mass that is abrogated by normalization of serum T3 levels. Endocrinology. 2013;154:4885-95 pubmed publisher..Normalizing serum T3 level by deleting deiodinase 1 corrects body composition and the metabolic alterations caused by the MCT8 deficiency. ..
- Osman D, Patterson C, Bailey K, Fisher K, Robinson N, Rigby S, et al. The copper supply pathway to a Salmonella Cu,Zn-superoxide dismutase (SodCII) involves P(1B)-type ATPase copper efflux and periplasmic CueP. Mol Microbiol. 2013;87:466-77 pubmed publisher..Copper binding by CueP in this pathogen may confer protection of the periplasm from copper-mediated damage while sustaining vital cupro-enzyme activity...
- Mades A, Gotthardt K, Awe K, Stieler J, Döring T, Füser S, et al. Role of human sec63 in modulating the steady-state levels of multi-spanning membrane proteins. PLoS ONE. 2012;7:e49243 pubmed publisher..Together, these results suggest that Sec63 may perform a substrate-selective quantity control function during cotranslational ER import. ..
- Kastelic V, Drobnic K. A single-nucleotide polymorphism (SNP) multiplex system: the association of five SNPs with human eye and hair color in the Slovenian population and comparison using a Bayesian network and logistic regression model. Croat Med J. 2012;53:401-8 pubmed..More samples from different populations should be analyzed before this assay could be used as one of the supplemental tools in tracing unknown individuals in more complicated crime investigations. ..
- Rose P, Fröbel J, Graumann P, Muller M. Substrate-dependent assembly of the Tat translocase as observed in live Escherichia coli cells. PLoS ONE. 2013;8:e69488 pubmed publisher..Our collective results are consistent with distinct Tat translocation sites dynamically forming in vivo in response to newly synthesized Tat substrates. ..
- Harty C, Römisch K. Analysis of Sec61p and Ssh1p interactions in the ER membrane using the split-ubiquitin system. BMC Cell Biol. 2013;14:14 pubmed publisher..We conclude that the split-ubiquitin system we used here has high fidelity, but low sensitivity and is of limited use for detection of new, transient interactions with protein translocation channels in the ER membrane. ..
- Hart K, Kimura S, Mushailov V, Budimlija Z, Prinz M, Wurmbach E. Improved eye- and skin-color prediction based on 8 SNPs. Croat Med J. 2013;54:248-56 pubmed..We can conclude that the new 8-plex system for the prediction of eye and skin color substantially enhances its former version. ..
- Setoodeh A, Haghighi A, Saleh Gohari N, Ellard S, Haghighi A. Identification of a SLC19A2 nonsense mutation in Persian families with thiamine-responsive megaloblastic anemia. Gene. 2013;519:295-7 pubmed publisher..697C>T (p.Gln233*) as the cause of the disease in all families. This mutation was previously reported in a Turkish patient with TRMA and is likely to be a founder mutation in the Persian population. ..
- Déjean G, Blanvillain Baufumé S, Boulanger A, Darrasse A, Dugé de Bernonville T, Girard A, et al. The xylan utilization system of the plant pathogen Xanthomonas campestris pv campestris controls epiphytic life and reveals common features with oligotrophic bacteria and animal gut symbionts. New Phytol. 2013;198:899-915 pubmed publisher..These similarities and our results support an important role for TBDTs and xylan utilization systems for bacterial adaptation in the phyllosphere, oligotrophic environments and animal guts. ..
- Hsieh Y, Zhang H, Wang H, Yang H, Jiang C, Sui S, et al. Reconstitution of functionally efficient SecA-dependent protein-conducting channels: transformation of low-affinity SecA-liposome channels to high-affinity SecA-SecYEG-SecDF·YajC channels. Biochem Biophys Res Commun. 2013;431:388-92 pubmed publisher
- Patzewitz E, Salcedo Sora J, Wong E, Sethia S, Stocks P, Maughan S, et al. Glutathione transport: a new role for PfCRT in chloroquine resistance. Antioxid Redox Signal. 2013;19:683-95 pubmed publisher..PfCRT has a dual role in CQR, facilitating both efflux of harmful CQ from the DV and influx of beneficial GSH into the DV. ..
- Fröbel J, Rose P, Lausberg F, Blümmel A, Freudl R, Muller M. Transmembrane insertion of twin-arginine signal peptides is driven by TatC and regulated by TatB. Nat Commun. 2012;3:1311 pubmed publisher..Collectively our results suggest that TatC is an insertase for twin-arginine signal peptides and that translocation-proficient signal sequence recognition requires the concerted action of TatC and TatB. ..
- Alcock F, Baker M, Greene N, Palmer T, Wallace M, Berks B. Live cell imaging shows reversible assembly of the TatA component of the twin-arginine protein transport system. Proc Natl Acad Sci U S A. 2013;110:E3650-9 pubmed publisher..In contrast to TatA-YFP, the oligomeric states of TatB-YFP and TatC-YFP fusions are not affected by substrate or the PMF, although TatB-YFP oligomerization does require TatC. ..
- Nies D, Herzberg M. A fresh view of the cell biology of copper in enterobacteria. Mol Microbiol. 2013;87:447-54 pubmed publisher..The data presented in the publication by Osman et?al. (2013) change our view of the cell biology of copper in enterobacteria...
- Chiabrando D, Marro S, Mercurio S, Giorgi C, Petrillo S, Vinchi F, et al. The mitochondrial heme exporter FLVCR1b mediates erythroid differentiation. J Clin Invest. 2012;122:4569-79 pubmed publisher..The aberrant expression of Flvcr1 isoforms may play a role in the pathogenesis of disorders characterized by an imbalance between heme and globin synthesis. ..
- Keseler I, Mackie A, Peralta Gil M, Santos Zavaleta A, Gama Castro S, Bonavides Martínez C, et al. EcoCyc: fusing model organism databases with systems biology. Nucleic Acids Res. 2013;41:D605-12 pubmed publisher..coli biology. The utility of EcoCyc is enhanced by new tools available on the EcoCyc web site, and the development of EcoCyc as a teaching tool is increasing the impact of the knowledge collected in EcoCyc...
- Lau C, Ishida H, Liu Z, Vogel H. Solution structure of Escherichia coli FeoA and its potential role in bacterial ferrous iron transport. J Bacteriol. 2013;195:46-55 pubmed publisher..These findings, together with bioinformatics and structural analyses, suggest that FeoA may have a different role, possibly interacting with the cytoplasmic domain of the highly conserved core portion of the FeoB transmembrane region...
- Owens C, Chim N, Goulding C. Insights on how the Mycobacterium tuberculosis heme uptake pathway can be used as a drug target. Future Med Chem. 2013;5:1391-403 pubmed publisher..We propose that inhibitor design could focus on heme analogs, with the goal of blocking specific steps of this pathway. In addition, heme uptake could be hijacked as a method of importing drugs into the mycobacterial cytosol. ..
- Ferrara A, Liao X, Gil Ibáñez P, Marcinkowski T, Bernal J, Weiss R, et al. Changes in thyroid status during perinatal development of MCT8-deficient male mice. Endocrinology. 2013;154:2533-41 pubmed publisher..This report is the first to identify that the ontogenesis of TH abnormalities in Mct8-deficient mice manifests with TH excess in the perinatal period...
- Ramasamy S, Abrol R, Suloway C, Clemons W. The glove-like structure of the conserved membrane protein TatC provides insight into signal sequence recognition in twin-arginine translocation. Structure. 2013;21:777-88 pubmed publisher..Correlating the biochemical literature to these results suggests that the signal sequence binds in this pocket, leading to structural changes that facilitate higher order assemblies. ..
- Walther T, Gottselig C, Grage S, Wolf M, Vargiu A, Klein M, et al. Folding and self-assembly of the TatA translocation pore based on a charge zipper mechanism. Cell. 2013;152:316-26 pubmed publisher..Similar "charge zippers" are proposed for other membrane-associated proteins, e.g., the biofilm-inducing peptide TisB, the human antimicrobial peptide dermcidin, and the pestiviral E(RNS) protein...
- Trueman S, Mandon E, Gilmore R. A gating motif in the translocation channel sets the hydrophobicity threshold for signal sequence function. J Cell Biol. 2012;199:907-18 pubmed publisher..Bioinformatic analysis indicates that the gating motif is conserved between eubacterial and archaebacterial SecY and eukaryotic Sec61. ..
- Owens C, Chim N, Graves A, Harmston C, Iniguez A, Contreras H, et al. The Mycobacterium tuberculosis secreted protein Rv0203 transfers heme to membrane proteins MmpL3 and MmpL11. J Biol Chem. 2013;288:21714-28 pubmed publisher..This work elucidates a key step in the mycobacterial uptake of heme, and it may be useful in the development of anti-tuberculosis drugs targeting this pathway...
- Rollauer S, Tarry M, Graham J, Jääskeläinen M, Jäger F, Johnson S, et al. Structure of the TatC core of the twin-arginine protein transport system. Nature. 2012;492:210-4 pubmed publisher..This structure provides a molecular description of the core of the Tat translocation system and a framework for understanding the unique Tat transport mechanism. ..
- Arenas J, Nijland R, Rodriguez F, Bosma T, Tommassen J. Involvement of three meningococcal surface-exposed proteins, the heparin-binding protein NhbA, the ?-peptide of IgA protease and the autotransporter protease NalP, in initiation of biofilm formation. Mol Microbiol. 2013;87:254-68 pubmed publisher..We conclude that NhbA and the ?-peptide of IgA protease are implicated in biofilm formation by binding eDNA and that NalP is an important regulator of this process through the proteolysis of these surface-exposed proteins. ..
- Sirish P, Ledford H, Timofeyev V, Thai P, Ren L, Kim H, et al. Action Potential Shortening and Impairment of Cardiac Function by Ablation of Slc26a6. Circ Arrhythm Electrophysiol. 2017;10: pubmed publisher..pHi is a critical regulator of other membrane and contractile proteins. Future studies are needed to investigate possible changes in these proteins in Slc26a6-/- mice. ..
- Hu J, Akula N, Wang N. Development of a Microemulsion Formulation for Antimicrobial SecA Inhibitors. PLoS ONE. 2016;11:e0150433 pubmed publisher..Overall, we have identified 11 potent SecA inhibitors using similarity search method. We have developed a microemulsion formulation for SecA inhibitors which improved the antimicrobial activities of SecA inhibitors. ..
- Huang C, Liu M, Kao H, Chou K, Yang A, Wang Y, et al. Effect of Alzheimer's Disease Risk Variant rs3824968 at SORL1 on Regional Gray Matter Volume and Age-Related Interaction in Adult Lifespan. Sci Rep. 2016;6:23362 pubmed publisher..These findings provide evidence that SORL1 rs3824968 modulates regional GM volume and is associated with brain trajectory during the adult lifespan. ..
- Hoffmann M, PfÃ¤nder Y, Tintel M, Masepohl B. Bacterial PerO Permeases Transport Sulfate and Related Oxyanions. J Bacteriol. 2017;199: pubmed publisher..capsulatus prototype, suggesting that PerO permeases form a conserved family whose members transport sulfate and related oxyanions. ..
- PÃ©rez Varela M, Corral J, Vallejo J, Rumbo Feal S, Bou G, Aranda J, et al. Mutations in the Î²-Subunit of the RNA Polymerase Impair the Surface-Associated Motility and Virulence of Acinetobacter baumannii. Infect Immun. 2017;85: pubmed publisher..baumannii ATCC 17978 strain, as well as in the virulent clinical isolate MAR002. Taken together, our results provide strong evidence of the connection between motility and virulence in this multiresistant nosocomial pathogen. ..
- Charlton Perkins M, Sendler E, Buschbeck E, Cook T. Multifunctional glial support by Semper cells in the Drosophila retina. PLoS Genet. 2017;13:e1006782 pubmed publisher..In addition to providing a new high-throughput model to study neuron-glia interactions, the fly eye will further help elucidate glial conserved "support networks" between invertebrates and vertebrates. ..
- Li H, Wang Q, Wang R, Zhang Y, Wang X, Wang H. Global regulator SoxR is a negative regulator of efflux pump gene expression and affects antibiotic resistance and fitness in Acinetobacter baumannii. Medicine (Baltimore). 2017;96:e7188 pubmed publisher..These results reveal that the global regulator SoxR is a negative regulator of efflux pump gene expression, and contributes to antibiotic resistance and fitness in A. baumannii. ..
- Kotsampasakou E, Montanari F, Ecker G. Predicting drug-induced liver injury: The importance of data curation. Toxicology. 2017;389:139-145 pubmed publisher..Finally, the model was implemented with open-source 2D RDKit descriptors in order to be provided to the community as a Python script. ..
- Shao J, Zhang M, Wang T, Li Y, Wang C. The roles of CDR1, CDR2, and MDR1 in kaempferol-induced suppression with fluconazole-resistant Candida albicans. Pharm Biol. 2016;54:984-92 pubmed publisher..Kaempferol-induced reversion in fluconazole-resistant C. albicans might be likely due to the suppression of the expression of CDR1, CDR2 and MDR1. ..
- Li L, Hu L, Han L, Ji H, Zhu Y, Wang X, et al. Expression of turtle riboflavin-binding protein represses mitochondrial electron transport gene expression and promotes flowering in Arabidopsis. BMC Plant Biol. 2014;14:381 pubmed publisher..The repressive effect on METC gene expression is not the only way by which RfBP induces H2O2 and currently unappreciated factors may also function under RfBP+ background. ..
- Verma R, Prabh N, Sankararamakrishnan R. New subfamilies of major intrinsic proteins in fungi suggest novel transport properties in fungal channels: implications for the host-fungal interactions. BMC Evol Biol. 2014;14:173 pubmed publisher..They can be attractive targets for developing anti-fungal drugs. The evolutionary pattern shared with their plant counterparts indicates possible involvement of new fungal MIPs in plant-fungi symbiosis and host-pathogen interactions. ..
- Nguyen L, Ma D, Shui G, Wong P, Cazenave Gassiot A, Zhang X, et al. Mfsd2a is a transporter for the essential omega-3 fatty acid docosahexaenoic acid. Nature. 2014;509:503-6 pubmed publisher..Our findings reveal an unexpected essential physiological role of plasma-derived LPCs in brain growth and function...
- Usami Y, Uemura S, Mochizuki T, Morita A, Shishido F, Inokuchi J, et al. Functional mapping and implications of substrate specificity of the yeast high-affinity leucine permease Bap2. Biochim Biophys Acta. 2014;1838:1719-29 pubmed publisher..This result suggests that the substrate partition efficiency to the buried Bap2 binding pocket is the primary determinant of substrate specificity rather than structural amino acid side chain recognition. ..
- Fribourg P, Chami M, Sorzano C, Gubellini F, Marabini R, Marco S, et al. 3D cryo-electron reconstruction of BmrA, a bacterial multidrug ABC transporter in an inward-facing conformation and in a lipidic environment. J Mol Biol. 2014;426:2059-69 pubmed publisher..Overall, the data are consistent with a mechanism of drug transport involving large conformational changes of BmrA and show that a bacterial ABC exporter can adopt at least two open inward conformations in lipid membrane...
- Canani R, Terrin G, Elce A, Pezzella V, Heinz Erian P, Pedrolli A, et al. Genotype-dependency of butyrate efficacy in children with congenital chloride diarrhea. Orphanet J Rare Dis. 2013;8:194 pubmed publisher..Australian New Zealand Clinical trial Registry ACTRN12613000450718. ..