calmodulin binding proteins


Summary: Proteins which bind calmodulin. They are found in many tissues and have a variety of functions including F-actin cross-linking properties, inhibition of cyclic nucleotide phosphodiesterase and calcium and magnesium ATPases.

Top Publications

  1. Lin J, Li Y, Eppinga R, Wang Q, Jin J. Chapter 1: roles of caldesmon in cell motility and actin cytoskeleton remodeling. Int Rev Cell Mol Biol. 2009;274:1-68 pubmed publisher
    ..Regulation of CaD level and its activity may provide a novel strategy for gene therapy. ..
  2. Riley M, Baker P, Tribe R, Taggart M. Expression of scaffolding, signalling and contractile-filament proteins in human myometria: effects of pregnancy and labour. J Cell Mol Med. 2005;9:122-34 pubmed
  3. Mirzapoiazova T, Kolosova I, Romer L, Garcia J, Verin A. The role of caldesmon in the regulation of endothelial cytoskeleton and migration. J Cell Physiol. 2005;203:520-8 pubmed
    ..These results suggest that CaD is critically involved in the regulation of the actin cytoskeleton and migration in EC, and that p38 MAPK-mediated CaD phosphorylation may be involved in endothelial cytoskeletal remodeling. ..
  4. Eves R, Webb B, Zhou S, Mak A. Caldesmon is an integral component of podosomes in smooth muscle cells. J Cell Sci. 2006;119:1691-702 pubmed
    ..In summary, this data suggests that caldesmon may play a role in the regulation of the dynamics of podosome assembly and that Ca2+/calmodulin may be part of a regulatory mechanism in podosome formation. ..
  5. Wang C. Caldesmon and the regulation of cytoskeletal functions. Adv Exp Med Biol. 2008;644:250-72 pubmed
    ..This chapter reviews the highlights of the past findings and summarizes the current views on this protein, with emphasis of its interaction with tropomyosin. ..
  6. Li Y, Zagato L, Kuznetsova T, Tripodi G, Zerbini G, Richart T, et al. Angiotensin-converting enzyme I/D and alpha-adducin Gly460Trp polymorphisms: from angiotensin-converting enzyme activity to cardiovascular outcome. Hypertension. 2007;49:1291-7 pubmed
  7. Huh K, DeMasi J, Ogawa H, Nakatani Y, Howley P, Munger K. Association of the human papillomavirus type 16 E7 oncoprotein with the 600-kDa retinoblastoma protein-associated factor, p600. Proc Natl Acad Sci U S A. 2005;102:11492-7 pubmed
    ..Therefore, p600 is a cellular target of E7 that regulates cellular pathways that contribute to anchorage-independent growth and cellular transformation. ..
  8. Larsson C. Protein kinase C and the regulation of the actin cytoskeleton. Cell Signal. 2006;18:276-84 pubmed
    ..Their potential role in PKC effects on the cytoskeleton is discussed. ..
  9. Yenerel M, Sundell I, Weese J, Bulger M, Gilligan D. Expression of adducin genes during erythropoiesis: a novel erythroid promoter for ADD2. Exp Hematol. 2005;33:758-66 pubmed
    ..The very high levels of expression of ADD2 suggest that its promoter may be useful for directing erythroid-specific gene expression. ..

More Information


  1. Mosevitsky M. Nerve ending "signal" proteins GAP-43, MARCKS, and BASP1. Int Rev Cytol. 2005;245:245-325 pubmed
    ..Special attention is devoted to a discussion of apparent inconsistencies in results and opinions of different authors concerning specific questions about the structure of proteins and their interactions. ..
  2. Kordowska J, Hetrick T, Adam L, Wang C. Phosphorylated l-caldesmon is involved in disassembly of actin stress fibers and postmitotic spreading. Exp Cell Res. 2006;312:95-110 pubmed
    ..These findings suggest that l-CaD phosphorylation plays an important role not only in cytoskeleton remodeling during cell shape changes, but also in cell spreading and migration. ..
  3. Manunta P, Lavery G, Lanzani C, Braund P, Simonini M, Bodycote C, et al. Physiological interaction between alpha-adducin and WNK1-NEDD4L pathways on sodium-related blood pressure regulation. Hypertension. 2008;52:366-72 pubmed publisher
    ..Relatively common alleles in the ADD1, WNK1, and NEDD4L genes when present in combination may have significant effects on renal sodium handling, BP, and antihypertensive response to thiazides. ..
  4. Eppinga R, Li Y, Lin J, Mak A, Lin J. Requirement of reversible caldesmon phosphorylation at P21-activated kinase-responsive sites for lamellipodia extensions during cell migration. Cell Motil Cytoskeleton. 2006;63:543-62 pubmed
    ..We conclude that phosphorylation of caldesmon by PAK is a dynamic process required to regulate actin dynamics and membrane protrusions in wound-induced cell migration. ..
  5. Yokouchi K, Numaguchi Y, Kubota R, Ishii M, Imai H, Murakami R, et al. l-Caldesmon regulates proliferation and migration of vascular smooth muscle cells and inhibits neointimal formation after angioplasty. Arterioscler Thromb Vasc Biol. 2006;26:2231-7 pubmed
    ..Overexpression of l-CaD suppressed cell growth and survival in VSMCs and inhibited neointimal formation after experimental angioplasty, partly by regulating the cytoskeletal tension-FAK-ERK axis. ..
  6. Morita T, Mayanagi T, Yoshio T, Sobue K. Changes in the balance between caldesmon regulated by p21-activated kinases and the Arp2/3 complex govern podosome formation. J Biol Chem. 2007;282:8454-63 pubmed
    ..Taken together, we conclude that in Rous sarcoma virus-transformed cells, changes in the balance between PAK1/2-regulated caldesmon and the Arp2/3 complex govern the formation of podosomes. ..
  7. Grosheva I, Vittitow J, Goichberg P, Gabelt B, Kaufman P, Borras T, et al. Caldesmon effects on the actin cytoskeleton and cell adhesion in cultured HTM cells. Exp Eye Res. 2006;82:945-58 pubmed
    ..Inducing such changes in the contractility and actin cytoskeleton of HTM cells in glaucomatous eyes in vivo could produce a therapeutically useful increase in outflow facility. ..
  8. Gotoh H, Okumura N, Yagi T, Okumura A, Shima T, Nagai K. Fyn-induced phosphorylation of beta-adducin at tyrosine 489 and its role in their subcellular localization. Biochem Biophys Res Commun. 2006;346:600-5 pubmed
    ..These results suggest that Fyn phosphorylates and binds to beta-adducin at Y489, resulting in translocation of beta-adducin to the Fyn-enriched regions in the plasma membrane. ..
  9. Bianchi G. Genetic variations of tubular sodium reabsorption leading to "primary" hypertension: from gene polymorphism to clinical symptoms. Am J Physiol Regul Integr Comp Physiol. 2005;289:R1536-49 pubmed
    ..The clinical effect size of adducin must be established also with the contribution of pharmacogenomics with a drug that selectively interferes with the sequence of events triggered by the mutated adducin. ..
  10. Li Y, Gallant C, Malek S, Morgan K. Focal adhesion signaling is required for myometrial ERK activation and contractile phenotype switch before labor. J Cell Biochem. 2007;100:129-40 pubmed
    ..These results point to focal adhesion signaling molecules as potential targets in the modulation of the myometrial contractility and the onset of labor. ..
  11. Rabenstein R, Addy N, Caldarone B, Asaka Y, Gruenbaum L, Peters L, et al. Impaired synaptic plasticity and learning in mice lacking beta-adducin, an actin-regulating protein. J Neurosci. 2005;25:2138-45 pubmed
    ..The current results indicate that beta-adducin may play an important role in the cellular mechanisms underlying activity-dependent synaptic plasticity associated with learning and memory. ..
  12. Guo H, Wang C. Specific disruption of smooth muscle caldesmon expression in mice. Biochem Biophys Res Commun. 2005;330:1132-7 pubmed
    ..The contractility of smooth muscles isolated from the knockout animals is currently under investigation. ..
  13. Bouche N, Yellin A, Snedden W, Fromm H. Plant-specific calmodulin-binding proteins. Annu Rev Plant Biol. 2005;56:435-66 pubmed
    ..The review also addresses aspects emerging from recent structural studies of CaM interactions with target proteins relevant to plants. ..
  14. Levy M, Wang Q, Kaspi R, Parrella M, Abel S. Arabidopsis IQD1, a novel calmodulin-binding nuclear protein, stimulates glucosinolate accumulation and plant defense. Plant J. 2005;43:79-96 pubmed
    ..As IQD1 is induced by mechanical stimuli, we propose IQD1 to be novel nuclear factor that integrates intracellular Ca(2+) signals to fine-tune glucosinolate accumulation in response to biotic challenge. ..
  15. Gu Z, Kordowska J, Williams G, Wang C, Hai C. Erk1/2 MAPK and caldesmon differentially regulate podosome dynamics in A7r5 vascular smooth muscle cells. Exp Cell Res. 2007;313:849-66 pubmed
    ..These results suggest that Erk1/2 and caldesmon differentially modulate PKC-mediated formation and/or dynamics of podosomes in A7r5 vascular smooth muscle cells. ..
  16. Yoshio T, Morita T, Kimura Y, Tsujii M, Hayashi N, Sobue K. Caldesmon suppresses cancer cell invasion by regulating podosome/invadopodium formation. FEBS Lett. 2007;581:3777-82 pubmed
    ..Conversely, the depletion of caldesmon facilitated the formation of podosomes/invadopodia and cell invasion. Taken together, our results indicate that caldesmon acts as a potent repressor of cancer cell invasion. ..
  17. Huang R, Li L, Guo H, Wang C. Caldesmon binding to actin is regulated by calmodulin and phosphorylation via different mechanisms. Biochemistry. 2003;42:2513-23 pubmed
    ..Taken together, these results demonstrate that while both Ca2+/CaM and MAPK phosphorylation govern CaD's function via a conformational change, the regulatory mechanisms are different. ..
  18. Li Y, Je H, Malek S, Morgan K. Role of ERK1/2 in uterine contractility and preterm labor in rats. Am J Physiol Regul Integr Comp Physiol. 2004;287:R328-35 pubmed
    ..We suggest that the ERK pathway could possibly be used to identify targets for the development of a novel class of tocolytic agents. ..
  19. Li Y, Wessels D, Wang T, Lin J, Soll D, Lin J. Regulation of caldesmon activity by Cdc2 kinase plays an important role in maintaining membrane cortex integrity during cell division. Cell Mol Life Sci. 2003;60:198-211 pubmed
    ..These results suggest that mitosis-specific phosphorylation of CaD plays a role in inhibiting bleb formation and that the N-terminal fragment of CaD is required for cell size determination. ..
  20. Merta M, Reiterova J, Stekrova J, Rysava R, Rihova Z, Tesar V, et al. Influence of the alpha-adducin and ACE gene polymorphism on the progression of autosomal-dominant polycystic kidney disease. Kidney Blood Press Res. 2003;26:42-9 pubmed
    ..The ACE and alpha-adducin polymorphisms do not play a significant role in the progression of ADPKD to ESRF. ..
  21. Tripodi G, Florio M, Ferrandi M, Modica R, Zimdahl H, Hubner N, et al. Effect of Add1 gene transfer on blood pressure in reciprocal congenic strains of Milan rats. Biochem Biophys Res Commun. 2004;324:562-8 pubmed
    ..In contrast, SBPs of Add2 and Add3 congenic strains were not different from those of the correspondent recipient parental strain. The fine mapping of chr14 congenic segment supports the identity of blood pressure QTL with Add1 gene...
  22. Foster D, Huang R, Hatch V, Craig R, Graceffa P, Lehman W, et al. Modes of caldesmon binding to actin: sites of caldesmon contact and modulation of interactions by phosphorylation. J Biol Chem. 2004;279:53387-94 pubmed
  23. Ali G, Reddy V, Lindgren P, Jakobek J, Reddy A. Differential expression of genes encoding calmodulin-binding proteins in response to bacterial pathogens and inducers of defense responses. Plant Mol Biol. 2003;51:803-15 pubmed
    ..Our results strongly suggest that at least five bean CBPs are involved in plant defense responses. ..
  24. Ju Z, Zhang H, Sun K, Song Y, Lu H, Hui R, et al. Alpha-adducin gene polymorphism is associated with essential hypertension in Chinese: a case-control and family-based study. J Hypertens. 2003;21:1861-8 pubmed
    ..This evidence indicates that the alpha-adducin gene may be a susceptible gene to essential hypertension. ..
  25. Perruc E, Charpenteau M, Ramirez B, Jauneau A, Galaud J, Ranjeva R, et al. A novel calmodulin-binding protein functions as a negative regulator of osmotic stress tolerance in Arabidopsis thaliana seedlings. Plant J. 2004;38:410-20 pubmed
    ..Thus, the AtCaMBP25 gene functions as a negative effector of osmotic stress tolerance and likely participates in stress signal transduction pathways. ..
  26. Bianchi G, Manunta P. Adducin, renal intermediate phenotypes, and hypertension. Hypertension. 2004;44:394-5 pubmed
  27. Li Y, Lin J, Reiter R, Daniels K, Soll D, Lin J. Caldesmon mutant defective in Ca(2+)-calmodulin binding interferes with assembly of stress fibers and affects cell morphology, growth and motility. J Cell Sci. 2004;117:3593-604 pubmed
    ..Together, these results demonstrate that CaD plays a crucial role in mediating the effects of Ca(2+)-CaM on the dynamics of the actin cytoskeleton during cell migration. ..
  28. Beeks E, van der Klauw M, Kroon A, Spiering W, Fuss Lejeune M, de Leeuw P. Alpha-adducin Gly460Trp polymorphism and renal hemodynamics in essential hypertension. Hypertension. 2004;44:419-23 pubmed
    ..The present study shows that the Trp460Trp genotype is significantly associated with reduced renal plasma flow and glomerular filtration rate as compared with the wild-type variant. ..
  29. Numaguchi Y, Huang S, Polte T, Eichler G, Wang N, Ingber D. Caldesmon-dependent switching between capillary endothelial cell growth and apoptosis through modulation of cell shape and contractility. Angiogenesis. 2003;6:55-64 pubmed
  30. Reddy V, Ali G, Reddy A. Characterization of a pathogen-induced calmodulin-binding protein: mapping of four Ca2+-dependent calmodulin-binding domains. Plant Mol Biol. 2003;52:143-59 pubmed
    ..Our data suggest a role for PICBP in Ca2+-mediated defense signaling and cell-death. Furthermore, PICBP is the first identified CBP in eukaryotes with four Ca2+-dependent CaM-binding domains. ..
  31. Zheng P, Sieuwerts A, Luider T, van der Weiden M, Sillevis Smitt P, Kros J. Differential expression of splicing variants of the human caldesmon gene (CALD1) in glioma neovascularization versus normal brain microvasculature. Am J Pathol. 2004;164:2217-28 pubmed
    ..The event coexists with tight junction (TJ) breakdown of the endothelial cells in glioma microvasculature. The data reveal a novel mechanism contributing to dysfunctionality of glioma neovascularization. ..
  32. Du L, Poovaiah B. A novel family of Ca2+/calmodulin-binding proteins involved in transcriptional regulation: interaction with fsh/Ring3 class transcription activators. Plant Mol Biol. 2004;54:549-69 pubmed
  33. Leidi M, Mariotti M, Maier J. Transcriptional coactivator EDF-1 is required for PPARgamma-stimulated adipogenesis. Cell Mol Life Sci. 2009;66:2733-42 pubmed publisher
    ..These results demonstrate that EDF-1 is required for PPARgamma transcriptional activation during 3T3-L1 differentiation. ..
  34. Hachani R, Dab H, Sakly M, Vicaut E, Callebert J, Sercombe R, et al. Influence of antagonist sensory and sympathetic nerves on smooth muscle cell differentiation in hypercholesterolemic rat. Auton Neurosci. 2010;155:82-90 pubmed publisher
    ..Our findings suggest that sympathectomy induces intimal thickening and favours SMC dedifferentiation, whereas sensory denervation favours SMC differentiation. ..
  35. Hornick J, Fletcher C. Sclerosing PEComa: clinicopathologic analysis of a distinctive variant with a predilection for the retroperitoneum. Am J Surg Pathol. 2008;32:493-501 pubmed publisher
    ..Sclerosing PEComas seem to pursue an indolent clinical course, unless associated with a frankly malignant component. Long-term follow-up will be required to confirm these findings...
  36. Chretien A, Dierick J, Delaive E, Larsen M, Dieu M, Raes M, et al. Role of TGF-beta1-independent changes in protein neosynthesis, p38alphaMAPK, and cdc42 in hydrogen peroxide-induced senescence-like morphogenesis. Free Radic Biol Med. 2008;44:1732-51 pubmed publisher
    ..This study further clarified the mechanisms of senescence-like morphogenesis in addition to the previously demonstrated role of TGF-beta1 signaling pathways. ..
  37. Schelleman H, Klungel O, Witteman J, Breteler M, Hofman A, van Duijn C, et al. Diuretic-gene interaction and the risk of myocardial infarction and stroke. Pharmacogenomics J. 2007;7:346-52 pubmed
    ..51; 95% CI: 0.23-1.15), whereas no interaction on the risk of stroke was found (SI: 0.84; 95% CI: 0.46-1.56). ..
  38. Pariser H, Herradon G, Ezquerra L, Perez Pinera P, Deuel T. Pleiotrophin regulates serine phosphorylation and the cellular distribution of beta-adducin through activation of protein kinase C. Proc Natl Acad Sci U S A. 2005;102:12407-12 pubmed
  39. Sleeman K, Baron M. The polymerase (L) protein of rinderpest virus interacts with the host cell protein striatin. Virology. 2005;332:225-34 pubmed
    ..The L domain with which striatin interacts was investigated by co-immunoprecipitation and striatin was shown to interact primarily with the central conserved domain. ..
  40. Compton L, Potash D, Mundell N, Barnett J. Transforming growth factor-beta induces loss of epithelial character and smooth muscle cell differentiation in epicardial cells. Dev Dyn. 2006;235:82-93 pubmed
    ..These data demonstrate that TGFbeta stimulates loss of epithelial character and smooth muscle differentiation in epicardial cells by means of a mechanism that requires ALK5 and p160 rho kinase. ..
  41. Bolognese F, Pitarque Martì M, Lo Cicero V, Mantovani R, Maier J. Characterization of the human EDF-1 minimal promoter: involvement of NFY and Sp1 in the regulation of basal transcription. Gene. 2006;374:87-95 pubmed
    ..Deletion of Sp1/Sp3 and NF-Y consensus sequences resulted in the total loss of EDF-1 promoter activity. Our studies indicate that Sp1 and NFY binding is essential for EDF-1 promoter activity. ..
  42. Costessi L, Devescovi G, Baralle F, Muro A. Brain-specific promoter and polyadenylation sites of the beta-adducin pre-mRNA generate an unusually long 3'-UTR. Nucleic Acids Res. 2006;34:243-53 pubmed
  43. Shan X, Hu J, Cayabyab F, Krieger C. Increased phospho-adducin immunoreactivity in a murine model of amyotrophic lateral sclerosis. Neuroscience. 2005;134:833-46 pubmed
  44. Wang L, Tsuda K, Sato M, Cohen J, Katagiri F, Glazebrook J. Arabidopsis CaM binding protein CBP60g contributes to MAMP-induced SA accumulation and is involved in disease resistance against Pseudomonas syringae. PLoS Pathog. 2009;5:e1000301 pubmed publisher
    ..These studies show that CBP60g constitutes a Ca(2+) link between MAMP recognition and SA accumulation that is important for resistance to P. syringae. ..
  45. Fliegmann J, Montel E, Djulic A, Cottaz S, Driguez H, Ebel J. Catalytic properties of the bifunctional soybean beta-glucan-binding protein, a member of family 81 glycoside hydrolases. FEBS Lett. 2005;579:6647-52 pubmed
    ..The substrate specificity, the mode of action, and the stereochemistry of the catalysis have been elucidated. This defines for the first time the inverting mode of the catalytic mechanism of glycoside hydrolases belonging to family 81. ..
  46. Agassandian K, Cassell M. Co-localization of caldesmon and calponin with cortical afferents, metabotropic glutamate and neurotrophic receptors in the lateral and central nuclei of the amygdala. Brain Res. 2008;1226:39-55 pubmed publisher
    ..Overall, these findings suggest that caldesmon and calponin in the amygdala are closely associated with afferents and receptors that have been strongly implicated in associative learning. ..
  47. Kuznetsova T, Citterio L, Herbots L, Carpini S, Thijs L, Casamassima N, et al. Effects of genetic variation in adducin on left ventricular diastolic function as assessed by tissue Doppler imaging in a Flemish population. J Hypertens. 2008;26:1229-36 pubmed publisher
    ..This association was more prominent in younger subjects in whom longstanding environmental factors and ageing are less likely to mask genetic effects. ..
  48. Yamaguchi N, Suzuki M, Fukaki H, Morita Terao M, Tasaka M, Komeda Y. CRM1/BIG-mediated auxin action regulates Arabidopsis inflorescence development. Plant Cell Physiol. 2007;48:1275-90 pubmed
    ..The severity of corymb-like inflorescence in crm1/big mutants correlated with increased levels of PIN1. Our results suggest that CRM1/BIG controls the elongation of the pedicels and stem internodes through auxin action. ..
  49. Bode Lesniewska B, Frigerio S, Exner U, Abdou M, Moch H, Zimmermann D. Relevance of translocation type in myxoid liposarcoma and identification of a novel EWSR1-DDIT3 fusion. Genes Chromosomes Cancer. 2007;46:961-71 pubmed
    ..Since the total number of patients is still limited, further studies are required to verify a putative association of type I FUS/DDIT3-fusion transcripts with a prognosis of MRCL...
  50. Kwasnicka Crawford D, Carson A, Scherer S. IQCJ-SCHIP1, a novel fusion transcript encoding a calmodulin-binding IQ motif protein. Biochem Biophys Res Commun. 2006;350:890-9 pubmed
    ..We show that IQCJ-SCHIP1 mRNA is highly expressed in the brain. Protein encoded by the IQCJ-SCHIP1 gene was localized to cytoplasm and actin-rich regions and in differentiated PC12 cells was also seen in neurite extensions. ..
  51. Yagita Y, Kitagawa K, Sasaki T, Terasaki Y, Todo K, Omura Matsuoka E, et al. Rho-kinase activation in endothelial cells contributes to expansion of infarction after focal cerebral ischemia. J Neurosci Res. 2007;85:2460-9 pubmed
    ..The vascular protective effect of Rho-kinase inhibitors may be useful in the treatment of the acute phase of ischemic stroke. ..
  52. Lambert M, Velasco P, Chang L, Viola K, Fernandez S, Lacor P, et al. Monoclonal antibodies that target pathological assemblies of Abeta. J Neurochem. 2007;100:23-35 pubmed
  53. McCluggage W, Boyde A. Uterine angioleiomyomas: a report of 3 cases of a distinctive benign leiomyoma variant. Int J Surg Pathol. 2007;15:262-5 pubmed
    ..Uterine angioleiomyoma has a close morphological resemblance to the similarly named skin tumor. We review uterine smooth-muscle and other mesenchymal neoplasms in which prominent vascular channels are a feature. ..