fimbriae proteins

Summary

Summary: Proteins that are structural components of bacterial fimbriae (FIMBRIAE, BACTERIAL) or sex pili (PILI, SEX).

Top Publications

  1. Nakata M, Kimura K, Sumitomo T, Wada S, Sugauchi A, Oiki E, et al. Assembly mechanism of FCT region type 1 pili in serotype M6 Streptococcus pyogenes. J Biol Chem. 2011;286:37566-77 pubmed publisher
  2. Giltner C, Nguyen Y, Burrows L. Type IV pilin proteins: versatile molecular modules. Microbiol Mol Biol Rev. 2012;76:740-72 pubmed publisher
    ..In this review, we consider recent advances in this research area, from structural revelations to insights into diversity, posttranslational modifications, regulation, and function. ..
  3. Henche A, Ghosh A, Yu X, Jeske T, Egelman E, Albers S. Structure and function of the adhesive type IV pilus of Sulfolobus acidocaldarius. Environ Microbiol. 2012;14:3188-202 pubmed publisher
    ..Our finding of an extraordinary stability of aap pili possibly represents an adaptation to harsh environments that S.?acidocaldarius encounters. ..
  4. Phan G, Remaut H, Wang T, Allen W, Pirker K, Lebedev A, et al. Crystal structure of the FimD usher bound to its cognate FimC-FimH substrate. Nature. 2011;474:49-53 pubmed publisher
    ..The FimD-FimC-FimH structure provides unique insights into the pilus subunit incorporation cycle, and captures the first view of a protein transporter in the act of secreting its cognate substrate. ..
  5. Zakrisson J, Wiklund K, Axner O, Andersson M. The shaft of the type 1 fimbriae regulates an external force to match the FimH catch bond. Biophys J. 2013;104:2137-48 pubmed publisher
  6. Wu C, Mishra A, Yang J, Cisar J, Das A, Ton That H. Dual function of a tip fimbrillin of Actinomyces in fimbrial assembly and receptor binding. J Bacteriol. 2011;193:3197-206 pubmed publisher
    ..Furthermore, recombinant FimQ, not FimP, bound surface-associated PRPs in a dose-dependent manner. Thus, not only does FimQ function as the major adhesin of the type 1 fimbriae, it also plays an important role in fimbrial assembly...
  7. Johnson J, Murphy C, Sippy J, Johnson T, Clegg S. Type 3 fimbriae and biofilm formation are regulated by the transcriptional regulators MrkHI in Klebsiella pneumoniae. J Bacteriol. 2011;193:3453-60 pubmed publisher
    ..This gene was found to be cotranscribed with a gene predicted to encode a PilZ domain-containing protein, designated MrkH. This protein was found to bind cyclic-di-GMP (c-di-GMP) and regulate type 3 fimbrial expression. ..
  8. Harfouche C, Filippini S, Gianfaldoni C, Ruggiero P, Moschioni M, Maccari S, et al. RrgB321, a fusion protein of the three variants of the pneumococcal pilus backbone RrgB, is protective in vivo and elicits opsonic antibodies. Infect Immun. 2012;80:451-60 pubmed publisher
    ..These results suggest that a vaccine composed of RrgB321 has the potential to cover 30% or more of all pneumococcal strains and support the inclusion of this fusion protein in a multicomponent vaccine against S. pneumoniae. ..
  9. Li J, Egelman E, Craig L. Structure of the Vibrio cholerae Type IVb Pilus and stability comparison with the Neisseria gonorrhoeae type IVa pilus. J Mol Biol. 2012;418:47-64 pubmed publisher
    ..The extraordinary stability of GC pili may represent an adaptation of the Type IVa pili to harsh environments and the need to retract against external forces...

More Information

Publications62

  1. Lemkul J, Bevan D. Characterization of interactions between PilA from Pseudomonas aeruginosa strain K and a model membrane. J Phys Chem B. 2011;115:8004-8 pubmed publisher
    ..Our results provide insight into the molecular events and energetics associated with pilus assembly, thereby initiating a detailed examination of the dynamics of this process. ..
  2. Zheng C, Wu J, Xie H. Differential expression and adherence of Porphyromonas gingivalis FimA genotypes. Mol Oral Microbiol. 2011;26:388-95 pubmed publisher
    ..We show that P. gingivalis strains with genotype I and II of FimA are efficient in interaction with saliva or S. gordonii. This work highlights the important role of FimA type I and II in P. gingivalis attachment to oral surfaces. ..
  3. Isidean S, Riddle M, Savarino S, Porter C. A systematic review of ETEC epidemiology focusing on colonization factor and toxin expression. Vaccine. 2011;29:6167-78 pubmed publisher
    ..However, heterogeneity across time, population, and region, confounded by variability in CF and toxin detection methodologies, obfuscates rational estimates for valency requirements. ..
  4. Ali S, Beckett E, Bae S, Navarre W. The 5.5 protein of phage T7 inhibits H-NS through interactions with the central oligomerization domain. J Bacteriol. 2011;193:4881-92 pubmed publisher
    ..The 5.5 proteins utilize a previously undescribed mechanism of H-NS antagonism that further highlights the critical importance that higher-order oligomerization plays in H-NS-mediated gene repression...
  5. Orrskog S, Rounioja S, Spadafina T, Gallotta M, Norman M, Hentrich K, et al. Pilus adhesin RrgA interacts with complement receptor 3, thereby affecting macrophage function and systemic pneumococcal disease. MBio. 2012;4:e00535-12 pubmed publisher
    ..These findings, together with the observed RrgA-dependent increase of intracellular survivors up to 10 h following macrophage infection, suggest that RrgA-CR3-mediated phagocytosis promotes systemic pneumococcal spread from local sites...
  6. El Mortaji L, Contreras Martel C, Moschioni M, Ferlenghi I, Manzano C, Vernet T, et al. The full-length Streptococcus pneumoniae major pilin RrgB crystallizes in a fibre-like structure, which presents the D1 isopeptide bond and provides details on the mechanism of pilus polymerization. Biochem J. 2012;441:833-41 pubmed publisher
    ..In conclusion, the crystal RrgB fibre is a compelling model for deciphering the molecular details required to generate the pneumococcal pilus. ..
  7. Geibel S, Waksman G. Crystallography and electron microscopy of chaperone/usher pilus systems. Adv Exp Med Biol. 2011;715:159-74 pubmed publisher
    ..Here we review the structural biology of the chaperone-usher pathway that has helped to understand the mechanisms by which biogenesis of an important class of bacterial organelles occurs. ..
  8. Leney A, Phan G, Allen W, Verger D, Waksman G, Radford S, et al. Second order rate constants of donor-strand exchange reveal individual amino acid residues important in determining the subunit specificity of pilus biogenesis. J Am Soc Mass Spectrom. 2011;22:1214-23 pubmed publisher
    ..The ESI-MS approach developed is applicable to the measurement of rates of DSE in pilus biogenesis in general and demonstrates the scope of ESI-MS in determining biomolecular processes in molecular detail. ..
  9. Moschioni M, De Angelis G, Harfouche C, Bizzarri E, Filippini S, Mori E, et al. Immunization with the RrgB321 fusion protein protects mice against both high and low pilus-expressing Streptococcus pneumoniae populations. Vaccine. 2012;30:1349-56 pubmed publisher
  10. Reunanen J, von Ossowski I, Hendrickx A, Palva A, de Vos W. Characterization of the SpaCBA pilus fibers in the probiotic Lactobacillus rhamnosus GG. Appl Environ Microbiol. 2012;78:2337-44 pubmed publisher
    ..rhamnosus GG compared to that of nonpiliated lactobacilli...
  11. Iebba V, Conte M, Lepanto M, Di Nardo G, Santangelo F, Aloi M, et al. Microevolution in fimH gene of mucosa-associated Escherichia coli strains isolated from pediatric patients with inflammatory bowel disease. Infect Immun. 2012;80:1408-17 pubmed publisher
    ..coli B2 phylogroup. In conclusion, we found some FimH variants that seem to be more involved than others in the evolution of IBD pathogenesis. ..
  12. Cehovin A, Simpson P, McDowell M, Brown D, Noschese R, Pallett M, et al. Specific DNA recognition mediated by a type IV pilin. Proc Natl Acad Sci U S A. 2013;110:3065-70 pubmed publisher
    ..Our findings illuminate the earliest step in natural transformation, reveal an unconventional mechanism for DNA binding, and suggest that selective DNA uptake is more widespread than previously thought. ..
  13. Guiton P, Cusumano C, Kline K, Dodson K, Han Z, Janetka J, et al. Combinatorial small-molecule therapy prevents uropathogenic Escherichia coli catheter-associated urinary tract infections in mice. Antimicrob Agents Chemother. 2012;56:4738-45 pubmed publisher
    ..Thus, we establish a proof-of-principle for the development of mannosides to prevent and eventually treat these infections in the face of rising antibiotic-resistant uropathogens...
  14. Malvankar N, Lovley D. Microbial nanowires: a new paradigm for biological electron transfer and bioelectronics. ChemSusChem. 2012;5:1039-46 pubmed publisher
  15. Franks A, Glaven R, Lovley D. Real-time spatial gene expression analysis within current-producing biofilms. ChemSusChem. 2012;5:1092-8 pubmed publisher
    ..This real-time reporter approach is likely to be a useful tool in optimizing the design of technologies relying on microbe-electrode interactions. ..
  16. Vengadesan K, Ma X, Dwivedi P, Ton That H, Narayana S. A model for group B Streptococcus pilus type 1: the structure of a 35-kDa C-terminal fragment of the major pilin GBS80. J Mol Biol. 2011;407:731-43 pubmed publisher
    ..We have also identified a pilin-like motif in the minor pilin GBS52, which might aid its incorporation at the pilus base. ..
  17. Jennings M, Jen F, Roddam L, Apicella M, Edwards J. Neisseria gonorrhoeae pilin glycan contributes to CR3 activation during challenge of primary cervical epithelial cells. Cell Microbiol. 2011;13:885-96 pubmed publisher
    ..To our knowledge, these are the first data to directly demonstrate how a protein-associated bacterial glycan may contribute to pathogenesis. ..
  18. Chamot Rooke J, Mikaty G, Malosse C, Soyer M, Dumont A, Gault J, et al. Posttranslational modification of pili upon cell contact triggers N. meningitidis dissemination. Science. 2011;331:778-82 pubmed publisher
    ..In turn, this regulated detachment process allowed propagation of the bacterium to new colonization sites and also migration across the epithelium, a prerequisite for dissemination and invasive disease. ..
  19. Yun S, Ji S, Jeon H, Wang X, Lee Y, Choi B, et al. A mutational study of Cnu reveals attractive forces between Cnu and H-NS. Mol Cells. 2012;33:211-6 pubmed publisher
    ..We propose that the attractive force that governs the Cnu-H-NS interaction is an ionic bond, unlike the hydrophobic interaction that is the major attractive force in most proteins. ..
  20. Wu C, Mishra A, Reardon M, Huang I, Counts S, Das A, et al. Structural determinants of Actinomyces sortase SrtC2 required for membrane localization and assembly of type 2 fimbriae for interbacterial coaggregation and oral biofilm formation. J Bacteriol. 2012;194:2531-9 pubmed publisher
  21. Hendrickx A, Budzik J, Oh S, Schneewind O. Architects at the bacterial surface - sortases and the assembly of pili with isopeptide bonds. Nat Rev Microbiol. 2011;9:166-76 pubmed publisher
    ..Together, these isopeptide bonds endow these sortase products with adhesive properties and resistance to host proteases. ..
  22. Nallapareddy S, Sillanpaa J, Mitchell J, Singh K, Chowdhury S, Weinstock G, et al. Conservation of Ebp-type pilus genes among Enterococci and demonstration of their role in adherence of Enterococcus faecalis to human platelets. Infect Immun. 2011;79:2911-20 pubmed publisher
  23. Hartung S, Arvai A, Wood T, Kolappan S, Shin D, Craig L, et al. Ultrahigh resolution and full-length pilin structures with insights for filament assembly, pathogenic functions, and vaccine potential. J Biol Chem. 2011;286:44254-65 pubmed publisher
    ..tularensis virulence. Combined results define a unified pilin architecture, specialized subdomain roles in pilus assembly and function, and potential therapeutic targets...
  24. Nielsen H, Guiton P, Kline K, Port G, Pinkner J, Neiers F, et al. The metal ion-dependent adhesion site motif of the Enterococcus faecalis EbpA pilin mediates pilus function in catheter-associated urinary tract infection. MBio. 2012;3:e00177-12 pubmed publisher
    ..Thus, this study identified the Ebp pilus as a virulence factor in E. faecalis CAUTI and also defined the molecular basis of this function, critical knowledge for the rational development of targeted therapeutics...
  25. Spirig T, Weiner E, Clubb R. Sortase enzymes in Gram-positive bacteria. Mol Microbiol. 2011;82:1044-59 pubmed publisher
    ..We also review recent data that suggest that sortases are targeted to specific sites on the cell surface where they work with other sortases and accessory factors to properly function. ..
  26. Cahoon L, Seifert H. Focusing homologous recombination: pilin antigenic variation in the pathogenic Neisseria. Mol Microbiol. 2011;81:1136-43 pubmed publisher
    ..We will also draw parallels to selected bacterial and eukaryotic antigenic variation systems, and suggest the most pressing unanswered questions related to understanding these important processes. ..
  27. Jen F, Warren M, Schulz B, Power P, Swords W, Weiser J, et al. Dual pili post-translational modifications synergize to mediate meningococcal adherence to platelet activating factor receptor on human airway cells. PLoS Pathog. 2013;9:e1003377 pubmed publisher
  28. Tammam S, Sampaleanu L, Koo J, Sundaram P, Ayers M, Chong P, et al. Characterization of the PilN, PilO and PilP type IVa pilus subcomplex. Mol Microbiol. 2011;82:1496-514 pubmed publisher
    ..Together these data demonstrate that PilN/PilO/PilP interact directly to form a stable heterotrimeric complex, explaining the dispensability of PilP's lipid anchor for localization and function...
  29. Paterson N, Baker E. Structure of the full-length major pilin from Streptococcus pneumoniae: implications for isopeptide bond formation in gram-positive bacterial pili. PLoS ONE. 2011;6:e22095 pubmed publisher
  30. Tchesnokova V, Aprikian P, Kisiela D, Gowey S, Korotkova N, Thomas W, et al. Type 1 fimbrial adhesin FimH elicits an immune response that enhances cell adhesion of Escherichia coli. Infect Immun. 2011;79:3895-904 pubmed publisher
    ..These observations have implications for the development of adhesin-specific vaccines and may serve as a paradigm for antibody-mediated enhancement of pathogen binding. ..
  31. Gentile M, Melchiorre S, Emolo C, Moschioni M, Gianfaldoni C, Pancotto L, et al. Structural and functional characterization of the Streptococcus pneumoniae RrgB pilus backbone D1 domain. J Biol Chem. 2011;286:14588-97 pubmed publisher
    ..Finally, we present a model of the RrgB protein architecture along with the mapping of two surface-exposed linear epitopes recognized by protective antisera. ..
  32. Yang Z, Hu W, Chen K, Wang J, Lux R, Zhou Z, et al. Alanine 32 in PilA is important for PilA stability and type IV pili function in Myxococcus xanthus. Microbiology. 2011;157:1920-8 pubmed publisher
    ..Characterization of this mutant and additional single-residue variants at this position in PilA demonstrated the critical role of alanine 32 in PilA stability, TFP assembly and retraction. ..
  33. Aprikian P, Interlandi G, Kidd B, Le Trong I, Tchesnokova V, Yakovenko O, et al. The bacterial fimbrial tip acts as a mechanical force sensor. PLoS Biol. 2011;9:e1000617 pubmed publisher
    ..Comparison to other structures suggests that this property is common in unrelated bacterial and eukaryotic adhesive complexes that must function in dynamic conditions. ..
  34. Szeto T, Dessen A, Pelicic V. Structure/function analysis of Neisseria meningitidis PilW, a conserved protein that plays multiple roles in type IV pilus biology. Infect Immun. 2011;79:3028-35 pubmed publisher
    ..These findings suggest that PilW is a multifunctional protein. ..
  35. Mishra A, Devarajan B, Reardon M, Dwivedi P, Krishnan V, Cisar J, et al. Two autonomous structural modules in the fimbrial shaft adhesin FimA mediate Actinomyces interactions with streptococci and host cells during oral biofilm development. Mol Microbiol. 2011;81:1205-20 pubmed publisher
    ..Thus, FimA should serve as an excellent paradigm for the development of therapeutic strategies and elucidating the precise molecular mechanisms underlying the interactions between cellular receptors and Gram-positive fimbriae...
  36. Wang W, Li G, Chen C, Xie X, Zhuang X. Chromosome organization by a nucleoid-associated protein in live bacteria. Science. 2011;333:1445-9 pubmed publisher
    ..Deleting H-NS led to substantial chromosome reorganization. These observations demonstrate that H-NS plays a key role in global chromosome organization in bacteria. ..
  37. Advani A, Gustafsson L, Ahrén C, Mooi F, Hallander H. Appearance of Fim3 and ptxP3-Bordetella pertussis strains, in two regions of Sweden with different vaccination programs. Vaccine. 2011;29:3438-42 pubmed publisher
    ..Our results highlight the importance of strain typing to gain insight into the mechanisms of immunity-associated selection of microbial subtypes and the causes of changes in incidences of infectious diseases...
  38. Becherelli M, Manetti A, Buccato S, Viciani E, Ciucchi L, Mollica G, et al. The ancillary protein 1 of Streptococcus pyogenes FCT-1 pili mediates cell adhesion and biofilm formation through heterophilic as well as homophilic interactions. Mol Microbiol. 2012;83:1035-47 pubmed publisher
    ..Finally, AP1_M6 knockout mutants were less virulent in mice, indicating that this protein is also implicated in GAS systemic infection. ..
  39. Jen F, Jones C, Wilson J, Schulz B, Jennings M. Substrate recognition of a structure motif for phosphorylcholine post-translational modification in Neisseria meningitidis. Biochem Biophys Res Commun. 2013;431:808-14 pubmed publisher
    ..We found that a single sequence of D-A-S after the disulphide bond of pilin protein is able to form a motif for ChoP modifications and the charge residue in this motif and the local structure are essential for the substrate recognition...
  40. Shin E, Park Y, Lee K, Langridge W, Lee J. Synthesis and assembly of Porphyromonas gingivalis fimbrial protein in potato tissues. Mol Biotechnol. 2009;43:138-47 pubmed publisher
    ..gingivalis. ..
  41. Carvalho F, Barnich N, Sivignon A, Darcha C, Chan C, Stanners C, et al. Crohn's disease adherent-invasive Escherichia coli colonize and induce strong gut inflammation in transgenic mice expressing human CEACAM. J Exp Med. 2009;206:2179-89 pubmed publisher
    ..Thus, targeting AIEC adhesion to gut mucosa represents a new strategy for clinicians to prevent and/or to treat ileal CD. ..
  42. Nguyen Y, Jackson S, Aidoo F, Junop M, Burrows L. Structural characterization of novel Pseudomonas aeruginosa type IV pilins. J Mol Biol. 2010;395:491-503 pubmed publisher
    ..However, based on our high-confidence model of group III PilA(PA14), compensatory changes allow for maintenance of a similar shape. ..
  43. Kuchma S, Ballok A, Merritt J, Hammond J, Lu W, Rabinowitz J, et al. Cyclic-di-GMP-mediated repression of swarming motility by Pseudomonas aeruginosa: the pilY1 gene and its impact on surface-associated behaviors. J Bacteriol. 2010;192:2950-64 pubmed publisher
    ..Our data indicate that PilY1 participates in multiple surface behaviors of P. aeruginosa, and we propose that PilY1 may act via regulation of SadC DGC activity but independently of altering global c-di-GMP levels. ..
  44. Kang H, Middleditch M, Proft T, Baker E. Isopeptide bonds in bacterial pili and their characterization by X-ray crystallography and mass spectrometry. Biopolymers. 2009;91:1126-34 pubmed publisher
    ..Crystallographic studies of pilin subunits have also led to the unexpected discovery of internal isopeptide crosslinks formed between lysine and asparagine residues. These, too, have been confirmed by mass spectrometry. ..
  45. Perez Chaparro P, Rouillon A, Minet J, Lafaurie G, Bonnaure Mallet M. fimA genotypes and PFGE profile patterns in Porphyromonas gingivalis isolates from subjects with periodontitis. Oral Microbiol Immunol. 2009;24:423-6 pubmed publisher
    ..To compare these techniques, we genotyped P. gingivalis clinical isolates as to (i) their fimA type and (ii) their whole genome restriction profile (PFGE analysis)...
  46. Bourgogne A, Thomson L, Murray B. Bicarbonate enhances expression of the endocarditis and biofilm associated pilus locus, ebpR-ebpABC, in Enterococcus faecalis. BMC Microbiol. 2010;10:17 pubmed publisher
    ..In conclusion, E. faecalis joins the growing family of pathogens that regulates virulence gene expression in response to bicarbonate and/or CO2. ..
  47. Tchesnokova V, McVeigh A, Kidd B, Yakovenko O, Thomas W, Sokurenko E, et al. Shear-enhanced binding of intestinal colonization factor antigen I of enterotoxigenic Escherichia coli. Mol Microbiol. 2010;76:489-502 pubmed publisher
  48. Bagaitkar J, Demuth D, Daep C, Renaud D, Pierce D, Scott D. Tobacco upregulates P. gingivalis fimbrial proteins which induce TLR2 hyposensitivity. PLoS ONE. 2010;5:e9323 pubmed publisher
    ..gingivalis phenotype in a manner likely to promote P. gingivalis colonization and infection while simultaneously reducing the host response to this major mucosal pathogen. ..
  49. Le Trong I, Aprikian P, Kidd B, Thomas W, Sokurenko E, Stenkamp R. Donor strand exchange and conformational changes during E. coli fimbrial formation. J Struct Biol. 2010;172:380-8 pubmed publisher
    ..The domains undergo a significant structural change in the donor strand binding groove during fimbrial assembly, and this likely plays a role in determining the specificity of subunit-subunit interactions among the fimbrial proteins. ..
  50. Saini S, Slauch J, Aldridge P, Rao C. Role of cross talk in regulating the dynamic expression of the flagellar Salmonella pathogenicity island 1 and type 1 fimbrial genes. J Bacteriol. 2010;192:5767-77 pubmed publisher
    ..The significance of these results is that they provide the first systematic study of the effect of regulatory cross talk on the expression dynamics of flagellar, SPI1, and type 1 fimbrial genes. ..
  51. Mossman K, Mian M, Lauzon N, Gyles C, Lichty B, MacKenzie R, et al. Cutting edge: FimH adhesin of type 1 fimbriae is a novel TLR4 ligand. J Immunol. 2008;181:6702-6 pubmed
    ..Our data suggest that TLR4 is a functional receptor for the adhesin portion of bacterial type 1 fimbria. ..
  52. Ong C, Beatson S, Totsika M, Forestier C, McEwan A, Schembri M. Molecular analysis of type 3 fimbrial genes from Escherichia coli, Klebsiella and Citrobacter species. BMC Microbiol. 2010;10:183 pubmed publisher
    ..In this study, we characterised the genes encoding type 3 fimbriae from CAUTI strains of Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Citrobacter koseri and Citrobacter freundii...
  53. Sillanpaa J, Nallapareddy S, Singh K, Prakash V, Fothergill T, Ton That H, et al. Characterization of the ebp(fm) pilus-encoding operon of Enterococcus faecium and its role in biofilm formation and virulence in a murine model of urinary tract infection. Virulence. 2010;1:236-46 pubmed
    ..faecium TX82 cell surface and provide the first evidence that pili of this emerging pathogen are important for its ability to form biofilm and to cause infection in an ascending UTI model...