Genomes and Genes
escherichia coli adhesins
Summary: Thin, filamentous protein structures, including proteinaceous capsular antigens (fimbrial antigens), that mediate adhesion of E. coli to surfaces and play a role in pathogenesis. They have a high affinity for various epithelial cells.
- Barnhart M, Pinkner J, Soto G, Sauer F, Langermann S, Waksman G, et al. PapD-like chaperones provide the missing information for folding of pilin proteins. Proc Natl Acad Sci U S A. 2000;97:7709-14 pubmed..This mechanism of small periplasmic chaperone function described here deviates from classical hsp60 chaperone-assisted folding. ..
- Anderson B, Ding A, Nilsson L, Kusuma K, Tchesnokova V, Vogel V, et al. Weak rolling adhesion enhances bacterial surface colonization. J Bacteriol. 2007;189:1794-802 pubmed
- Westerlund Wikström B, Korhonen T. Molecular structure of adhesin domains in Escherichia coli fimbriae. Int J Med Microbiol. 2005;295:479-86 pubmed..The existing structures give insight into the molecular basis of the diversity in fimbrial lectins. ..
- Amabile de Campos T, Stehling E, Ferreira A, Pestana de Castro A, Brocchi M, Dias da Silveira W. Adhesion properties, fimbrial expression and PCR detection of adhesin-related genes of avian Escherichia coli strains. Vet Microbiol. 2005;106:275-85 pubmed
- Szalo I, Goffaux F, Pirson V, Pierard D, Ball H, Mainil J. Presence in bovine enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli of genes encoding for putative adhesins of human EHEC strains. Res Microbiol. 2002;153:653-8 pubmed..Overall results showed that different genes encoding for putative adhesins of human EHEC strains are present in bovine EPEC and EHEC strains, but not one of them is present in all strains. ..
- Schilling J, Mulvey M, Vincent C, Lorenz R, Hultgren S. Bacterial invasion augments epithelial cytokine responses to Escherichia coli through a lipopolysaccharide-dependent mechanism. J Immunol. 2001;166:1148-55 pubmed..These results indicate that LPS is required for bacterial invasion to enhance host responses to E. coli within the bladder. ..
- Kariyawasam S, Wilkie B, Gyles C. Resistance of broiler chickens to Escherichia coli respiratory tract infection induced by passively transferred egg-yolk antibodies. Vet Microbiol. 2004;98:273-84 pubmed..coli used for challenge. Hence vaccination of broiler breeders to induce anti-PapG and anti-IutA antibodies may provide passive protection of progeny chicks against respiratory/septicemic disease caused by APEC. ..
- Mokady D, Gophna U, Ron E. Virulence factors of septicemic Escherichia coli strains. Int J Med Microbiol. 2005;295:455-62 pubmed..The variable profile of virulence genes in septicemic E. coli strains, as well as a prevalence of mobility-related sequences point out the existence of a "mix and match" combinatorial system. ..
- Hung C, Bouckaert J, Hung D, Pinkner J, Widberg C, DeFusco A, et al. Structural basis of tropism of Escherichia coli to the bladder during urinary tract infection. Mol Microbiol. 2002;44:903-15 pubmed..coli (EHEC) possess a sequence variation within the mannose-binding pocket of FimH, suggesting a naturally occurring mechanism of attenuation in EHEC bacteria that would prevent them from being targeted to the urinary tract. ..
- Nowrouzian F, Adlerberth I, Wold A. P fimbriae, capsule and aerobactin characterize colonic resident Escherichia coli. Epidemiol Infect. 2001;126:11-8 pubmed..The results indicate that not only P fimbriae, but also, certain capsules and the ability to produce the siderophore aerobactin might contribute to persistence of E. coli in the large intestine. ..
- Choudhury D, Thompson A, Stojanoff V, Langermann S, Pinkner J, Hultgren S, et al. X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli. Science. 1999;285:1061-6 pubmed..A donor strand complementation mechanism in which the chaperone donates a strand to complete the pilin domain explains the basis for both chaperone function and pilus biogenesis. ..
- Roux A, Beloin C, Ghigo J. Combined inactivation and expression strategy to study gene function under physiological conditions: application to identification of new Escherichia coli adhesins. J Bacteriol. 2005;187:1001-13 pubmed..coli to abiotic surfaces. The potential use of the RExBAD approach to study the function of cryptic or uncharacterized genes in large-scale postgenomic functional analyses is discussed. ..
- Poggio T, La Torre J, Scodeller E. Intranasal immunization with a recombinant truncated FimH adhesin adjuvanted with CpG oligodeoxynucleotides protects mice against uropathogenic Escherichia coli challenge. Can J Microbiol. 2006;52:1093-102 pubmed..Our results indicate that a recombinant truncated FimH adhesin plus CpG oligodeoxynucleotides is a suitable immunogenic combination that can contribute to the development of a highly efficacious urinary tract infection vaccine. ..
- Jones C, Pinkner J, Roth R, Heuser J, Nicholes A, Abraham S, et al. FimH adhesin of type 1 pili is assembled into a fibrillar tip structure in the Enterobacteriaceae. Proc Natl Acad Sci U S A. 1995;92:2081-5 pubmed..The bipartite nature of the type 1 pilus was also demonstrated on pili purified from clinical isolates of members of the Enterobacteriaceae family arguing that it is a conserved feature of the type 1 pilus. ..
- Schembri M, Hasman H, Klemm P. Expression and purification of the mannose recognition domain of the FimH adhesin. FEMS Microbiol Lett. 2000;188:147-51 pubmed..Here we report that a functional form of the FimH receptor-binding domain can be readily isolated and characterised by replacing the C-terminal domain with a histidine tag. ..
- Nowrouzian F, Wold A, Adlerberth I. Escherichia coli strains belonging to phylogenetic group B2 have superior capacity to persist in the intestinal microflora of infants. J Infect Dis. 2005;191:1078-83 pubmed..03). Thus, group B2 strains appear to possess yet unidentified traits that enhance their survival in the human intestine. ..
- Thomas W, Forero M, Yakovenko O, Nilsson L, Vicini P, Sokurenko E, et al. Catch-bond model derived from allostery explains force-activated bacterial adhesion. Biophys J. 2006;90:753-64 pubmed..This strongly suggests that FimH forms allosteric catch bonds. The model advances our understanding of both catch bonds and the role of allostery in regulating protein activity. ..
- Vandemaele F, Vandekerchove D, Vereecken M, Derijcke J, Dho Moulin M, Goddeeris B. Sequence analysis demonstrates the conservation of fimH and variability of fimA throughout avian pathogenic Escherichia coli (APEC). Vet Res. 2003;34:153-63 pubmed..This study elucidated the molecular structure and the degree of conservation of FimH and FimA among various avian pathogenic E. coli strains. ..
- Justice S, Lauer S, Hultgren S, Hunstad D. Maturation of intracellular Escherichia coli communities requires SurA. Infect Immun. 2006;74:4793-800 pubmed..Our data argue that SurA is required within bladder epithelial cells for UPEC to undergo the morphological changes that underlie IBC maturation and completion of the UTI pathogenic cascade. ..
- Eto D, Jones T, Sundsbak J, Mulvey M. Integrin-mediated host cell invasion by type 1-piliated uropathogenic Escherichia coli. PLoS Pathog. 2007;3:e100 pubmed..Cumulatively, these results indicate that beta1 and alpha3 integrins are functionally important receptors for type 1 pili-expressing bacteria within the urinary tract and possibly at other sites within the host...
- Wizemann T, Adamou J, Langermann S. Adhesins as targets for vaccine development. Emerg Infect Dis. 1999;5:395-403 pubmed..With recent advances in the identification, characterization, and isolation of other adhesins, similar approaches are being explored to prevent infections, from otitis media and dental caries to pneumonia and sepsis. ..
- Johnson J, Russo T. Molecular epidemiology of extraintestinal pathogenic (uropathogenic) Escherichia coli. Int J Med Microbiol. 2005;295:383-404 pubmed..Published by Elsevier GmbH. ..
- Sheikh J, Hicks S, Dall Agnol M, Phillips A, Nataro J. Roles for Fis and YafK in biofilm formation by enteroaggregative Escherichia coli. Mol Microbiol. 2001;41:983-97 pubmed..Our data do not suggest a role for type 1 fimbriae or motility in biofilm formation. EAEC appears to form a novel biofilm, which may be mediated solely by AAF and may reflect its interactions with the intestinal mucosa. ..
- Xie B, Zhou G, Chan S, Shapiro E, Kong X, Wu X, et al. Distinct glycan structures of uroplakins Ia and Ib: structural basis for the selective binding of FimH adhesin to uroplakin Ia. J Biol Chem. 2006;281:14644-53 pubmed..Results from this study shed light on the molecular pathogenesis of urinary tract infections and may aid in the design of glyco-mimetic inhibitors for preventing and treating this disease. ..
- Plançon L, du Merle L, Le Friec S, Gounon P, Jouve M, Guignot J, et al. Recognition of the cellular beta1-chain integrin by the bacterial AfaD invasin is implicated in the internalization of afa-expressing pathogenic Escherichia coli strains. Cell Microbiol. 2003;5:681-93 pubmed..Our results suggest that beta1 integrin is a common receptor for AfaD invasins and that additional AfaD-type-specific receptors exist. ..
- Nilsson L, Thomas W, Sokurenko E, Vogel V. Elevated shear stress protects Escherichia coli cells adhering to surfaces via catch bonds from detachment by soluble inhibitors. Appl Environ Microbiol. 2006;72:3005-10 pubmed..Shear stress-enhanced reduction of bacterial detachment has major physiological and therapeutic implications and needs to be considered when developing and screening drugs. ..
- Da Re S, Le Quéré B, Ghigo J, Beloin C. Tight modulation of Escherichia coli bacterial biofilm formation through controlled expression of adhesion factors. Appl Environ Microbiol. 2007;73:3391-403 pubmed..coli or those of other bacteria capable of being genetically manipulated may be of use both for reducing and for improving the impact of biofilms in a number of industrial and medical applications. ..
- Thomas W, Trintchina E, Forero M, Vogel V, Sokurenko E. Bacterial adhesion to target cells enhanced by shear force. Cell. 2002;109:913-23 pubmed..The ability of FimH to function as a force sensor provides a molecular mechanism for discrimination between surface-exposed and soluble receptor molecules. ..
- Thomas W, Nilsson L, Forero M, Sokurenko E, Vogel V. Shear-dependent 'stick-and-roll' adhesion of type 1 fimbriated Escherichia coli. Mol Microbiol. 2004;53:1545-57 pubmed..This 'stick-and-roll' adhesion could allow type 1 fimbriated bacteria to move along mannosylated surfaces under relatively low flow conditions and to accumulate preferentially in high shear regions. ..
- Martinez J, Hultgren S. Requirement of Rho-family GTPases in the invasion of Type 1-piliated uropathogenic Escherichia coli. Cell Microbiol. 2002;4:19-28 pubmed..Taken together, these data suggest that FimH-mediated invasion is dependent on GTP-binding protein activity that involves Cdc42 and PI 3-kinase activation probably upstream of Rac1. ..
- Zhou G, Mo W, Sebbel P, Min G, Neubert T, Glockshuber R, et al. Uroplakin Ia is the urothelial receptor for uropathogenic Escherichia coli: evidence from in vitro FimH binding. J Cell Sci. 2001;114:4095-103 pubmed..coli, and that the binding of uropathogenic bacteria to uroplakin Ia may play a key role in mediating the urothelial responses to bacterial attachment. ..
- Touaibia M, Wellens A, Shiao T, Wang Q, Sirois S, Bouckaert J, et al. Mannosylated G(0) dendrimers with nanomolar affinities to Escherichia coli FimH. ChemMedChem. 2007;2:1190-201 pubmed..Tetramer 4 had a K(d) of 0.45 nM. These clusters were 1000 times more potent than mannose for their capacity to inhibit the binding of E. coli to erythrocytes in vitro. ..
- Mossman K, Mian M, Lauzon N, Gyles C, Lichty B, MacKenzie R, et al. Cutting edge: FimH adhesin of type 1 fimbriae is a novel TLR4 ligand. J Immunol. 2008;181:6702-6 pubmed..Our data suggest that TLR4 is a functional receptor for the adhesin portion of bacterial type 1 fimbria. ..
- Khan N, Kim Y, Shin S, Kim K. FimH-mediated Escherichia coli K1 invasion of human brain microvascular endothelial cells. Cell Microbiol. 2007;9:169-78 pubmed..Taken together, these findings indicate that FimH induces host cell signalling cascades that are involved in E. coli K1 invasion of HBMEC and CD48 is a putative HBMEC receptor for FimH. ..
- Weissman S, Chattopadhyay S, Aprikian P, Obata Yasuoka M, Yarova Yarovaya Y, Stapleton A, et al. Clonal analysis reveals high rate of structural mutations in fimbrial adhesins of extraintestinal pathogenic Escherichia coli. Mol Microbiol. 2006;59:975-88 pubmed..coli are under strong positive selection (Dn/Ds > 1 for fimH and papG) for functionally adaptive amino acid replacements. ..
- Hanson M, Brinton C. Identification and characterization of E. coli type-1 pilus tip adhesion protein. Nature. 1988;332:265-8 pubmed..This protein is antigenically conserved among strains with different pilin serotypes and is located at the pilus tip. ..
- Barnhart M, Sauer F, Pinkner J, Hultgren S. Chaperone-subunit-usher interactions required for donor strand exchange during bacterial pilus assembly. J Bacteriol. 2003;185:2723-30 pubmed..Furthermore, the donor strand exchange assembly mechanism between subunits was found to be necessary for subunit translocation across the outer membrane usher. ..
- Dho Moulin M, Fairbrother J. Avian pathogenic Escherichia coli (APEC). Vet Res. 1999;30:299-316 pubmed..Hence, vaccination for colibacillosis is not widely practised because of the large variety of serogroups involved in field outbreaks. ..
- Hoepelman I, Meiland R, Langermann S. [Candidate vaccine against urinary tract infections]. Ned Tijdschr Geneeskd. 2001;145:1860-2 pubmed..In animal studies and in a phase 1 study, this vaccine has proven to be both immunogenic and safe. In this era of increasing resistance to antibiotics, such a method of prevention is of high importance. ..
- Krogfelt K, Bergmans H, Klemm P. Direct evidence that the FimH protein is the mannose-specific adhesin of Escherichia coli type 1 fimbriae. Infect Immun. 1990;58:1995-8 pubmed..Furthermore, we show by receptor immunoelectron microscopy that the FimH protein is located laterally in the structure of the type 1 fimbriae. ..
- Marc D, Dho Moulin M. Analysis of the fim cluster of an avian O2 strain of Escherichia coli: serogroup-specific sites within fimA and nucleotide sequence of fimI. J Med Microbiol. 1996;44:444-52 pubmed..In addition, the sequence of the previously uncharacterised gene fimI, which is located between genes fimA and fimC, was determined. ..
- Aprikian P, Tchesnokova V, Kidd B, Yakovenko O, Yarov Yarovoy V, Trinchina E, et al. Interdomain interaction in the FimH adhesin of Escherichia coli regulates the affinity to mannose. J Biol Chem. 2007;282:23437-46 pubmed..This shift provides a mechanism for FimH-mediated shear-enhanced adhesion by enabling the adhesin to form catch bond-like interactions that are longer lived at high tensile force. ..
- Hahn E, Wild P, Hermanns U, Sebbel P, Glockshuber R, Häner M, et al. Exploring the 3D molecular architecture of Escherichia coli type 1 pili. J Mol Biol. 2002;323:845-57 pubmed
- Bouckaert J, Mackenzie J, de Paz J, Chipwaza B, Choudhury D, Zavialov A, et al. The affinity of the FimH fimbrial adhesin is receptor-driven and quasi-independent of Escherichia coli pathotypes. Mol Microbiol. 2006;61:1556-68 pubmed..Our results imply that the carbohydrate expression profile of targeted host tissues and of natural inhibitors in urine, such as Tamm-Horsfall protein, are stronger determinants of adhesion than FimH variation. ..
- Bouckaert J, Berglund J, Schembri M, De Genst E, Cools L, Wuhrer M, et al. Receptor binding studies disclose a novel class of high-affinity inhibitors of the Escherichia coli FimH adhesin. Mol Microbiol. 2005;55:441-55 pubmed..coli. We foresee that our findings will spark new ideas and initiatives for the development of UTI vaccines and anti-adhesive drugs to prevent anticipated and recurrent UTIs. ..
- Vandemaele F, Ververken C, Bleyen N, Geys J, D Hulst C, Addwebi T, et al. Immunization with the binding domain of FimH, the adhesin of type 1 fimbriae, does not protect chickens against avian pathogenic Escherichia coli. Avian Pathol. 2005;34:264-72 pubmed..No protective effects of the FimH156 could be detected, confirming the results of the first experiment. Thus, although the FimH156 induced a strong immune response, it was unable to protect chickens against APEC. ..
- Min G, Stolz M, Zhou G, Liang F, Sebbel P, Stoffler D, et al. Localization of uroplakin Ia, the urothelial receptor for bacterial adhesin FimH, on the six inner domains of the 16 nm urothelial plaque particle. J Mol Biol. 2002;317:697-706 pubmed
- Hritonenko V, Kostakioti M, Stathopoulos C. Quaternary structure of a SPATE autotransporter protein. Mol Membr Biol. 2006;23:466-74 pubmed..Implications of our findings on the mechanism of autotransporter secretion across the outer membrane are discussed. ..
- Johnson J, Brown J, Carlino U, Russo T. Colonization with and acquisition of uropathogenic Escherichia coli as revealed by polymerase chain reaction-based detection. J Infect Dis. 1998;177:1120-4 pubmed..These methods should facilitate the studies needed to clarify the relative contributions of these three pathways to the pathogenesis of urinary tract infection. ..
- Johnson J, Brown J, Ahmed P. Diversity of hemagglutination phenotypes among P-fimbriated wild-type strains of Escherichia coli in relation to papG allele repertoire. Clin Diagn Lab Immunol. 1998;5:160-70 pubmed..coli strains can be reproducibly assessed by a microtiter HA assay and that they correspond broadly to papG genotype but in a more complex and varied fashion than previously recognized. ..
- Brooks D, Cavanagh J, Jayroe D, Janzen J, Snoek R, Trust T. Involvement of the MN blood group antigen in shear-enhanced hemagglutination induced by the Escherichia coli F41 adhesin. Infect Immun. 1989;57:377-83 pubmed..Collectively, these data strongly suggest that glycophorin AM is a receptor for the F41 adhesin. ..